CN1103665A - Chinese herb medicine extraction by enzymolysis - Google Patents
Chinese herb medicine extraction by enzymolysis Download PDFInfo
- Publication number
- CN1103665A CN1103665A CN 93120161 CN93120161A CN1103665A CN 1103665 A CN1103665 A CN 1103665A CN 93120161 CN93120161 CN 93120161 CN 93120161 A CN93120161 A CN 93120161A CN 1103665 A CN1103665 A CN 1103665A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- extraction
- enzyme
- amylase
- polygalacturonase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
In the present invention, different enzyme combination and usage is adopted based on the medicinal property of specific Chinese medicines. By using specific catalysis and mild reaction condition, effective components of Chinese medicine are extracted. The present invention is suitable for both single and compound Chinese medicines.
Description
The present invention relates to and in the producing of Chinese medicine, use zymin, force catalystic, fermentative to extract middle pharmaceutically active ingredient, for the biological extraction of Chinese medicine preparation provides a novel process.
Chinese materia medica is an important wealth of world medicine treasure-house.Chinese medicine preparation is of long standing and well established, and soup starts from (B.C. 1766) before the Shang dynasty.The modern Chinese herbal medicine preparation has one to overlap leach extraction method comparatively complete, system, as decocting method, percolation, reflux extraction etc.The purified method comprises water extraction and alcohol precipitation method, ethanol extract from water precipitation, liquid-liquid extraction method, acid-base method, salting-out process, dialysis method, sieve method, polymeric amide absorption method, ion exchange method, crystallization process etc.Xiao Helong has invented (publication number CN1064888A. application number 92102469.Y. volume issue 8-40) edible mushrooms and herbal medicine mixed fermentation brewing process.
The objective of the invention is to: utilize characteristics such as strong, gentle action condition of biological enzyme formulation catalytic rate height, specificity and nontoxicity, a large amount of herbal medicine is carried out serial enzymes such as catalyzed degradation, synthetic, transfer and turn usefulness into, improve the extraction yield and the utilization ratio of Chinese medicine with this, promote the development of Chinese drugs agentia production technology.
The protein with katalysis that is produced by cell is called enzyme.According to the type of various enzyme institute catalyzed reaction, enzyme can be divided into six big classes, and wherein: the hydrolase major part belongs to extracellular enzyme, it is the widest to distribute in vivo, quantity is maximum, and industrial production and application are also the most extensive, as cellulase, polygalacturonase, amylase, proteolytic enzyme, nuclease etc.Because the composition of some biologically active generally is low molecular compound or can be dissociated into the ionic material at the aqueous solution in the Chinese medicine extract, so all can use lytic enzyme to general Chinese medicine.According to cellulase-→ polygalacturonase-→ amylase-→ proteolytic enzyme-→ order of nuclease, then can be with Mierocrystalline cellulose firm in structure in the root of Chinese medicine (draft, xylophyta), stem, leaf, flower, fruit, the seed, degradeds step by step such as the pectin of height fatization and pectic acid, starch, albumen, nucleic acid finally generate all kinds of low molecular compounds.Otherwise Chinese medicine has the polysaccharide, protein, tannin of therapeutic action etc., then mainly uses cellulase and polygalacturonase, and as need nucleic acid degradation product Nucleotide, nucleosides and phosphoric acid etc., then emphasis uses proteolytic enzyme and nuclease.The effect of a kind of enzyme of single use is unsatisfactory, must the logotype plurality of enzymes.As substrate, then must understand and study the structure of matter and the characteristic of this Chinese medicine with specific Chinese medicine, could select then with histaminase to its catalyzed degradation.
The present invention compares the advantage that is had with prior art and is: can use the enzyme of various combination to carry out catalyzed degradation according to the characteristic of Chinese medicine and be polymer or low molecular compound; Because the mild action condition of enzyme, the thermo-labile and volatile component of part is kept in degradation process; Nontoxic, the no strong acid of Chinese drugs agentia through enzyme extraction, highly basic; Extraction yield and utilization ratio are all above more than 30% of conventional method.
Embodiment:
Example 1: Enzymatic Extraction Flos Lonicerae chlorogenic acid
One, 7 times of distilled water to Japanese Honeysuckle are added in the dried Japanese Honeysuckle fragmentation that fresh nothing is gone mouldy, and contain in the stainless steel fermentor tank.
Two, add 3 ‰ cellulases, 2 ‰ polygalacturonases, 32 ℃-45 ℃ of PH5.5～6.5 temperature, fermentation time 120 minutes.Aerobic stirs.Filter, filtrate is stand-by.
Three, adding distil water is equivalent to for the first time 70% in the filter residue, adds a-amylase, beta-amylase each 2 ‰, PH5.5～6.5,60 ℃-70 ℃ of temperature, 80 minutes time.Aerobic stirs.Filter, filtrate is stand-by.
Four, filter residue again adding distil water be equivalent to primaryly 70%, add neutral protease 3 ‰, PH7～7.5,38 ℃-45 ℃ of temperature, 60 minutes time.Aerobic stirs.Filter, filtrate is stand-by.
Five, above-mentioned three filtrates are mixed, and vacuum decompression concentrates, 60 ℃ of temperature.
Six, ethanol sedimentation, ethanol content 60%, 72 hours time, normal temperature.Filter residue precipitation twice, the smart filter of gained solution.Vacuum decompression reclaims ethanol.Can.Pasteurization.
Example 2: Poria fungus nucleome Enzymatic Extraction Pachymose
One, the Poria fungus nucleome that fresh nothing is gone mouldy is broken into 20 orders.Add 5 times of distilled water, contain in the stainless steel fermentor tank to the Poria fungus nucleome.
Two, add 5 ‰ cellulases, PH5.5～6.5,32 ℃-45 ℃ of temperature, fermentation time 120 minutes.Aerobic stirs.Filter, filtrate is stand-by.
Three, add 3 ‰ polygalacturonases, PH5.0-6.0,28 ℃-40 ℃ of temperature, 80 minutes time, aerobic stirs.Filter, filtrate is stand-by.
Four, add 3 ‰ cellulases, the same binomial of fermentation process.
Five, above-mentioned three filtrates are mixed, and vacuum decompression concentrates, 60 ℃ of temperature.
Six, freezing precipitation, 3 ℃ of temperature, 24 hours time.Smart filter.Can.Pasteurization.
Claims (2)
1, with the height katalysis of enzyme, specificity, gentle action condition and nontoxicity selectively, integrated use in traditional Chinese medicine extraction, extract the effective component of cmm of simple prescription and compound.
2,, integrated use described selectively according to claim 1 is characterized in that general series of Chinese medicine Enzymatic Extraction and order: cellulase-→ polygalacturonase-→ amylase-→ proteolytic enzyme-→ nuclease.Chinese medicine macromolecular cpd Enzymatic Extraction is mainly used cellulase, polygalacturonase, amylase.Nucleic acid degradation product Enzymatic Extraction emphasis uses proteolytic enzyme and nuclease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93120161A CN1058293C (en) | 1993-12-08 | 1993-12-08 | Chinese herb medicine extraction by enzymolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93120161A CN1058293C (en) | 1993-12-08 | 1993-12-08 | Chinese herb medicine extraction by enzymolysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1103665A true CN1103665A (en) | 1995-06-14 |
| CN1058293C CN1058293C (en) | 2000-11-08 |
Family
ID=4993188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93120161A Expired - Fee Related CN1058293C (en) | 1993-12-08 | 1993-12-08 | Chinese herb medicine extraction by enzymolysis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1058293C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103330924A (en) * | 2013-06-07 | 2013-10-02 | 朱民生 | Biological extraction method of traditional Chinese medicine activin |
| CN103598325A (en) * | 2013-11-13 | 2014-02-26 | 王玉清 | Health-care poria cocos yoghourt and preparation method thereof |
| CN103621906A (en) * | 2013-11-12 | 2014-03-12 | 汪盛明 | Chufa and leonurus pseudomacranthus rice crust |
| CN104257767A (en) * | 2014-09-19 | 2015-01-07 | 西藏藏真堂藏药产业有限公司 | Method for extracting active ingredients of rheum australe and application thereof |
| CN107412275A (en) * | 2017-05-11 | 2017-12-01 | 张新 | The preparation method of a variety of biology enzyme micro mist fermentation preparations |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1053361A (en) * | 1990-01-17 | 1991-07-31 | 大连生物化学制药厂 | Thromboembolism preventing blood fat reducing buccal tablet and production method thereof |
-
1993
- 1993-12-08 CN CN93120161A patent/CN1058293C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103330924A (en) * | 2013-06-07 | 2013-10-02 | 朱民生 | Biological extraction method of traditional Chinese medicine activin |
| CN103330924B (en) * | 2013-06-07 | 2015-07-29 | 朱民生 | A kind of biological extraction process of active Chinese drug component element |
| CN103621906A (en) * | 2013-11-12 | 2014-03-12 | 汪盛明 | Chufa and leonurus pseudomacranthus rice crust |
| CN103598325A (en) * | 2013-11-13 | 2014-02-26 | 王玉清 | Health-care poria cocos yoghourt and preparation method thereof |
| CN104257767A (en) * | 2014-09-19 | 2015-01-07 | 西藏藏真堂藏药产业有限公司 | Method for extracting active ingredients of rheum australe and application thereof |
| CN107412275A (en) * | 2017-05-11 | 2017-12-01 | 张新 | The preparation method of a variety of biology enzyme micro mist fermentation preparations |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1058293C (en) | 2000-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105273934B (en) | A kind of fermented type Opuntia ficus wine and its production method | |
| CN103719880B (en) | Preparation method of high-activity purple sweet potato dietary fiber | |
| CN104013657B (en) | A kind of American ginseng medicine extracts after saponin(e microbial fermentation extracting method again | |
| CN101412703B (en) | Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid | |
| CN105559089A (en) | Liver-protecting and toxin-expelling fruit and vegetable enzyme and preparation method thereof | |
| CN101085958A (en) | Tartary buckwheat oxidation resistance health care wine | |
| CN101171956A (en) | Method for extracting polyoses from bagasse | |
| CN101649286A (en) | Method for preparing hawthorn fruit vinegar | |
| CN102304501A (en) | Complex enzyme preparation and application and method thereof for extracting plant polysaccharides by using same | |
| CN102344872B (en) | Method for preparing sweet potato yellow wine containing anthocyanidin | |
| CN107184966A (en) | A kind of pharmaceutical composition for preventing and treating diabetes and its complication | |
| CN113398159A (en) | Lithocarpus litseifolius extract particles and preparation method and application thereof | |
| CN1058293C (en) | Chinese herb medicine extraction by enzymolysis | |
| CN102492666A (en) | Enzyme preparation, application and method for extracting pueraria flavonid by enzyme preparation thereof | |
| CN109010505A (en) | A kind of biological enzyme digestion method of fructus lycii | |
| CN1884571B (en) | Process for extracting polysaccharide from lycoris plant | |
| CN1416843A (en) | Enzyme prepn method of preparing Chinese medicine | |
| CN107259260A (en) | Chinese wolfberry fruit vinegar beverage that a kind of fall fire helps digestion and preparation method thereof | |
| CN114668797B (en) | Plant fermentation liquid for resisting oxidation, reducing blood fat and protecting liver as well as preparation method and application thereof | |
| CN115820783A (en) | Preparation method for obtaining multiple triterpenoids by fermenting rosa roxburghii tratt | |
| CN1718708A (en) | Sisat hemp stem health care dry wine and its preparation method | |
| CN111602813B (en) | Preparation method of sunflower disc fermentation stock solution for health food and product thereof | |
| CN112877157A (en) | Preparation method of radix puerariae wine | |
| CN1028715C (en) | Process for forced extraction of mushroom juice with enzyme preparation | |
| CN101085051A (en) | Method for fermenting and preparing fleece flower root |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |