CN101085051A - Method for fermenting and preparing fleece flower root - Google Patents
Method for fermenting and preparing fleece flower root Download PDFInfo
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- CN101085051A CN101085051A CN 200710065990 CN200710065990A CN101085051A CN 101085051 A CN101085051 A CN 101085051A CN 200710065990 CN200710065990 CN 200710065990 CN 200710065990 A CN200710065990 A CN 200710065990A CN 101085051 A CN101085051 A CN 101085051A
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- polygoni multiflori
- radix polygoni
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Abstract
The invention relates to a method for processing Polygonum multiflorum by utilizing fungi fermentation. The inventive method comprises the following steps: slicing or pulverizing Polygonum multiflorum, putting in a container, adding water for fully humidification or preparing into pulp, innoculating strain of cultured Mucor or Rhizopus oryzae, and culturing at 15-37deg.C for 3-7 days. Technical matters for solving in the invention is to degrade nocuous anthraquinone in Polygonum multiflorum through microorganism fermentation method and effectively reserve stilbene glucoside, thereby in effective reservation of pharmacological action of Polygonum multiflorum removing noxious constituent of Polygonum multiflorum and improving medicinal value of Polygonum multiflorum.
Description
Affiliated technical field: the invention belongs to Chinese medicine processing and biotransformation field
Technical background
Normally a kind of mixture of Chinese medicine had both contained effective ingredient, also contained toxic component.The process of preparing Chinese medicine is the important means that alleviates herbal toxic effect.Traditional Chinese medicine processing method mainly be steam by adding various adjuvant, boil, physics or chemical method such as stir-fry.These traditional concocting methods do not have selectivity to the destruction of toxic component, and promptly effective ingredient and toxic component are usually simultaneously destroyed, even toxic component is more stable, and effective ingredient is easier to be destroyed, and then influence drug effect.Along with the quickening of internationalization of tcm process, popularization has in the world caused bad influence to the toxic and side effects of Chinese medicine to Chinese medicine.Specificity ground degraded toxic component wherein in the very complicated composition of Chinese medicine how, the composition of remaining valid simultaneously is unaffected, and the use value that improves Chinese medicine is had crucial meaning.
Microorganism can produce abundant enzyme system, utilizes the enzyme specificity ground of microorganisms the toxic component in the Chinese medicine is transformed targetedly or to be degraded, and is the important means [Wang Xinghong etc., Chinese herbal medicine, 2001,32 (3): 267-268] of realization this purpose.Some enzymes such as cellulase, pectase also usually are used to handle Chinese medicine, increase the extraction ratio of certain useful component, but do not relate to degraded or detoxification to toxic component.Also the someone promotes some composition of Chinese medicine to increase or increased activity by inoculating specific bacterial strain.Employing fermentation methods such as Dai Wansheng are handled Radix Et Rhizoma Rhei, and the dissociated anthraquinone constituents content in the fermentation Radix Et Rhizoma Rhei has increased by 6 times (Yunnan Chinese medicine magazine, 2005 years 01 phases).He Chen etc. concoct the thrombus dissolving effect (CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2005 the 5th phases) that Flos Carthami has strengthened Flos Carthami with fermentation of bacillus.Though fermentation method is also arranged in traditional concocting method, mainly be the physiological activity that utilizes medical material itself, as Fructus Hordei Germinatus etc.Or utilize air or the contained microorganism of medical material itself on medical material, to grow, formed some new property of medicine, as Massa Medicata Fermentata, Pinellia leaven etc.But these methods all are with the drug effect that improves medical material or to change the property of medicine be target, do not relate to the degraded of specific toxic component.
Radix Polygoni Multiflori is the dry root of polygonum multiflorum thunb (Polygonum multiflorum Thunb.), the title that four big tonic herbs are arranged, be one of China's large Chinese crude drug commonly used and main exit medical material, have effects such as the body's immunity of adjusting, blood fat reducing, atherosclerosis, promotion adrenal cortex function, its effective ingredient is the stilbene glycosides compound, and the anthraquinone analog compound that contains in the Radix Polygoni Multiflori is the main toxic component of its diarrhea inducing.Often there is the Radix Polygoni Multiflori Preparata concocted time not enough clinically, causes stomachache, diarrhoea case to take place.Multiflower knotweed tuber prepared core is to remove its cathartic side effect, thereby strengthens the function of enriching yin and nourishing kidney.The technology that conventional removal Radix Polygoni Multiflori rushes down side effect down is very complicated, the concocting method in successive dynasties steams, kind [Li Yikui surplus water from washing rice system, processed with wine, parch etc. 30, Jiang Mingying. pharmacology of Chinese materia medica (the 1st edition). Beijing: China Traditional Chinese Medicine Publishing House, 1992:198.], the record that Compendium of Material Medica has multiflower knotweed tuber prepared " it is to use that nine steamings nine are shone ".Modern age Radix Polygoni Multiflori concocting method in also increased methods such as Radix Rehmanniae Preparata juice steams, the Semen sojae atricolor Rhizoma Zingiberis Recens boils.Chinese Pharmacopoeia version in 2005 is recorded black juice steam, steamed two kinds of steaming methods.Modern study is the result show, the Radix Polygoni Multiflori sheet will steam 32 hours ability and remove discharge function under the normal pressure, and under the high pressure steaming (120 ℃), sheet steams 4 hours, and piece steams the discharge function of 6 hours ability removal Radix Polygoni Multiflori.Radix Polygoni Multiflori after steaming is except that total sugar, free type anthraquinone component increase, and combined anthraquinone composition, phospholipid, stilbene glucoside content then obviously descend, and have had a strong impact on the drug effect of Radix Polygoni Multiflori.
The invention provides a kind of Radix Polygoni Multiflori effective ingredient that neither influences, the wherein concocting method of toxic component anthraquinone analog compound of degrading again simultaneously specificity.
Summary of the invention
The objective of the invention is to overcome the defective of prior art, provide a kind of, keep the method for effective ingredient stilbene glucoside in the Radix Polygoni Multiflori simultaneously fully by anthraquinone analog compound in the microbial fermentation approach specificity ground degraded Radix Polygoni Multiflori.
Fermentation of the present invention is concocted the method for Radix Polygoni Multiflori medical material and is made up of following step:
One, the Radix Polygoni Multiflori medicinal liquid of 5%-15% will be mixed with after the dried Radix Polygoni Multiflori pulverizing; Or Radix Polygoni Multiflori thinly sliced, admix the Testa Tritici of 5%-30%, add moisture and make it fully moistening; Or breaking into slurry after fresh Radix Polygoni Multiflori added entry, 121 ℃ of sterilizations 15-150 minute connect bacterium, fermentation after the cooling.
Two, the bacterial strain that is connect is Rhizopus oryzae, Mucor.Cultivated 3-7 days under 15-37 ℃ of condition the inoculation back.
Three, the Radix Polygoni Multiflori medical material after fermentation finishes can be directly as medicinal, and perhaps dry back is preserved standby.
The purpose that adds moisture in step 1 is to make Radix Polygoni Multiflori fully moistening, is convenient to growth of microorganism and transforms, and addition is generally more than 1 times of Radix Polygoni Multiflori dry weight, perhaps is made into the slurry that contains the 5-10% Radix Polygoni Multiflori powder.The purpose that adds Testa Tritici is to make medical material more loose, more helps the growth of bacterium.
Radix Polygoni Multiflori medical material of the present invention can be fresh Radix Polygoni Multiflori root, direct slicing or break into slurry; Also can be exsiccant Radix Polygoni Multiflori medical material, directly break into the powdered thing that to cross the 30-200 mesh sieve, add medical material weight 1-1.5 water doubly, make solid culture and use; Also can add medical material weight 5-7 water doubly, make liquid culture; Thinly slice shape after also wettable.Described rhizopus, Mucor can directly be adopted commercially available distillers yeast kind or homemade solid kind or liquid strain, and its consumption is 0.01%-10%.
Rhizopus of the present invention, mucormycosis contain the enzyme or the ability of the anthraquinone analog compound in the specificity ground degraded Radix Polygoni Multiflori, make that anthraquinone analog compound content reduces significantly in the Radix Polygoni Multiflori, rather than as traditional multiflower knotweed tuber prepared method, total anthraquinone content does not change, and just combined anthraquinone is converted into free type anthraquinone.This two classes fungus does not contain the enzyme of the stilbene glucoside of degrading, and can farthest keep the effective ingredient stilbene glucoside in the Radix Polygoni Multiflori, and the Radix Polygoni Multiflori after the processing can cause that not as traditional concocting method stilbene glucoside destroys significantly.
The technical problem to be solved in the present invention is to pass through microbial fermentation, with the anthraquinone class diarrhea inducing composition degraded that contains in the Radix Polygoni Multiflori, keep effective ingredient stilbene glucoside in the Radix Polygoni Multiflori simultaneously, reached the drug effect that has both kept Radix Polygoni Multiflori, simultaneously, remove the toxic component of Radix Polygoni Multiflori again, improved the medical value of Radix Polygoni Multiflori.
This technology is made up of following feature: Radix Polygoni Multiflori is in the fermentation concocting process, and fine powder or slurry must be thinly sliced or break into to Radix Polygoni Multiflori, and the microorganism of being convenient to be inoculated fully acts on; The Mucor or the Rhizopus oryzae strain of necessary seeded with pure must be cultivated certain hour (3-7 days) after the inoculation.
Beneficial effect is: by the specificity degraded of bacterial strains such as Rhizopus oryzae, Mucor to anthraquinone component, reduced the discharge function of Radix Polygoni Multiflori.Simultaneously, such bacterial strain does not have destruction to Radix Polygoni Multiflori effective ingredient stilbene glucoside, has farthest kept the drug effect of Radix Polygoni Multiflori.
For a better understanding of the present invention, describe with experimental data below:
Radix Polygoni Multiflori powder is broken into the powder that to cross 60 mesh sieves, be mixed with 300 milliliters of 5% Radix Polygoni Multiflori powder solution, pack in 3 500 milliliters the vial, every bottled amount is 100 milliliters, 121 ℃ of autoclaving 15min, Rhizopus oryzae (Rhizopus oryzae) or Mucor (Mucor.sp) strain are inserted in the Radix Polygoni Multiflori powder fermentation medium, and 28 ℃ of 300rpm cultivated 3 days.After the fermentation ends Radix Polygoni Multiflori fermentation liquid packed in the enamel tray, in 65 ℃ of forced air dryings or microwave drying, collect dry thing, through the high pressure liquid chromatograph assay determination, general anthraquinone amount in the fermented product drops to the 30-50% of crude drug, and stilbene glucoside content remains unchanged.The Radix Polygoni Multiflori of fermentation our experiments show that, the zest of animal intestinal is obviously reduced.
Specific embodiment:
1, Radix Polygoni Multiflori powder is broken into the powder that to cross 80 mesh sieves, be mixed with 300 milliliters of solution that contain 5% Radix Polygoni Multiflori powder, pack in 3 500 milliliters the vial, every bottled amount is 100 milliliters, 121 ℃ of autoclaving 15min, Rhizopus oryzae (Rhizopus oryzae) or Mucor (Mucor.sp) strain are inserted in the Radix Polygoni Multiflori powder fermentation medium, and 28 ℃ of 300rpm cultivated 3 days.After the fermentation ends Radix Polygoni Multiflori fermentation liquid packed in the enamel tray,, collect dry thing, promptly can be used as after qualified after testing medicinal in 65 ℃ of forced air dryings or microwave drying.
2,1 kilogram of fresh Radix Polygoni Multiflori undercut is become the thick thin slice of 1mm, in the enamel tray of packing into, cover 2 layers of gauze, the 121 ℃ of autoclaving 20min of autoclave that pack into insert strain on the Radix Polygoni Multiflori thin slice, 28 ℃ aerobic culture 4-5 days.After the fermentation ends with Radix Polygoni Multiflori pieces in 65 ℃ of forced air dryings, can use after qualified after testing.
3, Radix Polygoni Multiflori is pulverized, allocated into 20% Testa Tritici, add the water of Radix Polygoni Multiflori weight 120%, mix thoroughly, 100 ℃ of normal-pressure sterilizations are 2 hours in the tray of packing into, the wheat bran strain of Rhizopus oryzae or Mucor is admixed in the Radix Polygoni Multiflori powder culture medium, 28 ℃ aerobic culture 4-6 days.The Radix Polygoni Multiflori fermented product in 65 ℃ of forced air dryings, is collected dry thing, and sieving gets final product.
Claims (4)
1, a kind of new method of fermenting and preparing fleece flower root is characterized in that this method with fresh Radix Polygoni Multiflori section or pulping, maybe beats powder with dried Radix Polygoni Multiflori, adds water, and by behind a certain amount of inoculation strain, a period of time of fermenting is at a certain temperature formed.
2, fermenting and preparing fleece flower root method according to claim 1 is characterized in that the strain that connects is Mucor, Rhizopus oryzae.
3, the method for fermenting and preparing fleece flower root according to claim 1 and 2, strain weight (or volume) percentage ratio that it is characterized in that inserting Radix Polygoni Multiflori is 0.01-10%.
4,, it is characterized in that described temperature is 15-37 ℃, incubation time 3-7 days according to claim 1 or 2 or 3 described fermenting and preparing fleece flower root methods.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200710065990 CN101085051A (en) | 2007-06-26 | 2007-06-26 | Method for fermenting and preparing fleece flower root |
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| CN 200710065990 CN101085051A (en) | 2007-06-26 | 2007-06-26 | Method for fermenting and preparing fleece flower root |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186816A (en) * | 2014-07-04 | 2014-12-10 | 贵州七合生物科技有限公司 | Radix polygoni multiflori tea substitute and production method thereof |
| CN107041903A (en) * | 2016-02-05 | 2017-08-15 | 中国中医科学院中药研究所 | Prepared fleece flower root Habitat producing processes integrated new method |
| CN108096362A (en) * | 2018-01-16 | 2018-06-01 | 重庆顶尚生物制品有限责任公司 | A kind of black hair Chinese medicine preparation and method for fermenting, extracting and preparing |
-
2007
- 2007-06-26 CN CN 200710065990 patent/CN101085051A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186816A (en) * | 2014-07-04 | 2014-12-10 | 贵州七合生物科技有限公司 | Radix polygoni multiflori tea substitute and production method thereof |
| CN107041903A (en) * | 2016-02-05 | 2017-08-15 | 中国中医科学院中药研究所 | Prepared fleece flower root Habitat producing processes integrated new method |
| CN107041903B (en) * | 2016-02-05 | 2021-03-09 | 中国中医科学院中药研究所 | Integrated new method for processing and concocting radix Polygoni Multiflori Preparata in producing area |
| CN108096362A (en) * | 2018-01-16 | 2018-06-01 | 重庆顶尚生物制品有限责任公司 | A kind of black hair Chinese medicine preparation and method for fermenting, extracting and preparing |
| CN108096362B (en) * | 2018-01-16 | 2020-09-18 | 重庆顶尚生物制品有限责任公司 | Hair-blacking traditional Chinese medicine preparation and fermentation extraction preparation method |
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Open date: 20071212 |