CN110354317B

CN110354317B - Preparation method of embedding liquid and preparation method of water-soluble human body lubricating liquid

Info

Publication number: CN110354317B
Application number: CN201910779659.4A
Authority: CN
Inventors: 叶剑生; 桂茂林
Original assignee: Foshan Pingchuang Medical Technology Co ltd
Current assignee: Foshan Pingchuang Medical Technology Co ltd
Priority date: 2019-08-22
Filing date: 2019-08-22
Publication date: 2022-02-11
Anticipated expiration: 2039-08-22
Also published as: CN110354317A

Abstract

A preparation method of embedding liquid and preparation method of water-soluble human body lubricating fluid, the preparation method of its embedding liquid is through step S1-S9, prepare and carry the Chinese medicinal extract embedding liquid, can make the product embed more fully and steadily and reduce the loss of active ingredient, has lengthened the life cycle of the product, compared with general Chinese medicinal preparation, this method can lengthen about 1.5 years' expiry date; through the steps S10-S12, the high-temperature high-pressure high-speed homogeneous carbomer is adopted, so that the product is more uniform and fine when in use, the original effect of the product lubricity is improved, and the absorption of the effective components of the traditional Chinese medicine is promoted to further promote the effect of the medicine.

Description

Preparation method of embedding liquid and preparation method of water-soluble human body lubricating liquid

Technical Field

The invention relates to the technical field of water-soluble human body lubricating liquid, in particular to a preparation method of embedding liquid and a preparation method of water-soluble human body lubricating liquid.

Background

The human body lubricating liquid is a product with a lubricating function and made of medicinal materials, and can be used for leading an inspection instrument into a natural cavity of a human body in the field of medical inspection, such as gastroscopy, rectal examination and the like. In other fields such as lubrication in sexual life using sex toys and lubrication in sexual life. The traditional human body lubricating fluid is prepared by adopting oily or water-soluble chemical substances according to a certain formula. The water soluble lubricating liquid is used in human body and can be diluted by human body secretion liquid and discharged to body, and its performance is superior to that of oil soluble lubricating liquid. However, the water-soluble lubricating fluid is difficult to mask some defects existing when the components of the water-soluble lubricating fluid are artificially synthesized chemical substances when the water-soluble lubricating fluid achieves excellent lubricating property and water solubility. Such as irritation and sensitization of the product to the body contact parts; the product has extremely limited repairing effect on mucous membrane damage and red swelling caused by accidental friction during the introduction of the device.

Disclosure of Invention

The invention aims to provide a preparation method of embedding liquid, which can prepare the embedding liquid with traditional Chinese medicine extracts.

The invention also provides a preparation method of the water-soluble human body lubricating liquid, and the preparation method uses the embedding liquid with the traditional Chinese medicine extract.

In order to achieve the purpose, the invention adopts the following technical scheme:

a preparation method of embedding liquid comprises the following steps:

s1, adding water into honeysuckle and angelica to obtain aromatic water a by a steam distillation method;

s2, mixing the residue filtered in the step S1 with rhubarb and dark plum, adding water, and cooking;

s3, filtering with a screen to obtain filtrate b and dregs, and decocting the dregs with water; filtering the cooked mixture by using a screen to obtain filtrate c;

s4, combining the filtrate b and the filtrate c to obtain a total filtrate, precipitating the total filtrate by absolute ethyl alcohol and filtering the precipitate by a screen; obtaining final filtrate c;

s5, slowly adding aromatic water a into the final filtrate c under stirring to obtain Chinese medicinal extract X, and sealing at 5 + -2 deg.C for storage;

s6, mixing hydroxyethyl fiber and polyvinylpyrrolidone, dissolving in water, and stirring at high pressure and temperature of 100-120 ℃ for reaction;

s7, controlling the reaction temperature of the hydroxyethyl fibers and the polyvinylpyrrolidone to be 30-50 ℃, and enabling the dispersion liquid to be in a uniform state;

s8, putting the traditional Chinese medicine extract X in the step S5 into the dispersion liquid in the step S7, and stirring and mixing at high pressure and 30-50 ℃;

s9, cooling to a probing point temperature below 10 ℃ after stirring is stopped, and hermetically storing in a high-pressure environment to obtain an embedding solution of the traditional Chinese medicine extract.

More specifically, in step S1, 1 part of honeysuckle and 1 part of angelica are added with 7 to 11 times of water in a mixing amount to obtain aromatic water a by steam distillation;

the step S2 is specifically that the residue left after filtration in the step S1 is mixed with 1 part of rhubarb and 1 part of dark plum, and then water with the amount of 7-11 times of the mixed amount is added for cooking.

In addition, the screen meshes used in the steps S3 and S4 are 280-320 meshes.

A method for preparing water-soluble human body lubricating fluid comprises the following steps: a preparation method of embedding liquid and a mixing method of transparent thin gel;

a method for producing the embedding liquid according to any one of claims 1 to 3;

the mixing method of the transparent thin gel comprises the following steps:

s10, mixing glycerol and carbomer with water, stirring at high pressure and temperature of 100-120 ℃ until the mixture is homogenized and dispersed;

s11, adding triethanolamine under the same pressure and temperature, and stirring until the mixture is transparent; then cooling to a detection temperature of 30-50 ℃ under the condition of constant pressure to obtain transparent dilute gel;

s12, mixing the Chinese medicine extract embedding liquid prepared in the step S9 and the transparent dilute gel prepared in the step S11; stirring and adding water under high pressure, normal temperature and vacuum state; stirring to obtain water-soluble human body lubricating liquid.

Further, the water-soluble human body lubricant comprises: 10-40% of traditional Chinese medicine extract X, 0.1-0.2% of hydroxyethyl fiber, 0.3-0.5% of polyvinylpyrrolidone, 0.10-0.15% of carbomer, 0.1-0.2% of triethanolamine, 15-25% of glycerol and the balance of water.

More specifically, in the step S12, the embedding solution of the herbal extract prepared in the step S9 is mixed with the lucent thin gel prepared in the step S11; stirring and adding water under high pressure, normal temperature and vacuum state; stirring and then ultrasonically oscillating to obtain the water-soluble human body lubricating liquid.

Further, the preparation method is prepared by a kettle-in-kettle structure of a reaction kettle;

the kettle-in-kettle structure of the reaction kettle comprises: the device comprises an outer reaction kettle, an inner kettle feeder and a reaction processor;

at least two inner reaction kettles are arranged in the outer reaction kettle; the inner reaction kettle is provided with an inner kettle feeding hole and an inner kettle discharging hole, and the inner kettle discharging hole is positioned at the bottom of the inner reaction kettle; the inner kettle feeder is arranged at the feeding hole of the inner kettle and communicated with the reaction cavity of the inner reaction kettle and the outside of the outer reaction kettle, and the discharging hole of the inner kettle is communicated with the reaction cavity of the inner reaction kettle and the reaction cavity of the outer reaction kettle; the inner kettle feeder and the inner kettle discharge port are respectively provided with an inner kettle switch; the reaction processor is arranged in the outer reaction kettle and/or the inner reaction kettle and is used for adjusting the reaction environment of the outer reaction kettle and/or the inner reaction kettle.

Further illustratively, the reaction processor comprises: a high temperature heater;

the heating end of the high-temperature heater is in contact with the outer reaction kettle and/or the inner reaction kettle, and the high-temperature heater is used for heating the inner part of the outer reaction kettle and/or the inner reaction kettle.

Further illustratively, the reaction processor comprises: a cryogenic liquid flow channel;

the low-temperature liquid flow channel surrounds the outer side of the outer reaction kettle and/or the inner reaction kettle and is used for introducing low-temperature liquid to lower the temperature of the outer reaction kettle and/or the inner reaction kettle.

Further illustratively, the reaction processor comprises: a high-speed wall breaking device;

the high-speed broken wall device includes: a wall breaking driver and a wall breaking cutter;

the stiff end of broken wall driver install in the outer reation kettle and/or in the interior reation kettle, the broken wall driver is used for driving the rotation of broken wall cutter.

The invention has the beneficial effects that:

the invention adopts the extraction technology for purifying the effective components, unique embedding of the traditional Chinese medicine extract, dispersing the embedding liquid, mixing and filling the traditional Chinese medicine extract at medium temperature, and low-temperature stabilization, so that the product can be embedded more fully and stably, the loss of the effective components can be reduced, the service life of the product can be prolonged, and compared with a common traditional Chinese medicine extract preparation, the method can prolong the effective period by about 1.5 years.

Drawings

FIG. 1 is a schematic diagram of the tank structure in a reaction tank;

FIG. 2 is a schematic diagram of the construction of an internal kettle feeder;

wherein:

the device comprises an outer reaction kettle 1, an inner reaction kettle 2, an inner kettle feeder 3, an outer kettle feeder 7 and a low-temperature liquid flow channel 9;

an outer kettle feed inlet 11, an outer kettle discharge outlet 12 and an outer kettle switch 13;

an inner kettle feed inlet 21, an inner kettle discharge outlet 22 and an inner kettle switch 23;

a high-temperature heater 41, an ultrasonic device 43 and a high-speed wall breaking device 44;

a feeding shell 32, a feeding screw rod 33, a motor mounting seat 31 and a feeding motor 34;

a wall breaking driver 441 and a wall breaking cutter 442.

Detailed Description

The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.

A preparation method of embedding liquid comprises the following steps:

The Chinese medicine extract is fully and stably filled in the three-dimensional grid of the high polymer material;

furthermore, the embedding liquid is used for embedding the traditional Chinese medicine extract, has a microcapsule structure, and ensures that the active ingredients of the traditional Chinese medicine extract, namely the honeysuckle, the angelica, the rhubarb and the dark plum, are better protected and more stable, and can play a role of slow release and increase the action time when in use. The product adopts a classic formula for astringing, promoting granulation and removing necrotic tissue, and adopts a unique process to stably and fully embed the traditional Chinese medicine extract in a chemically synthesized high polymer material; compared with the water-soluble human body lubricant prepared by common chemical synthetic substances, the produced water-soluble human body lubricant has the advantages that the stimulation and sensitization probability is reduced by 80 percent; the repairing effect on the already caused injury and red swelling can reach 100 percent. Meanwhile, the product has the faint scent of the traditional Chinese medicine aromatic water, and the sensory effect of a user is improved. The rhubarb, the angelica and the ethanol for purification in the traditional Chinese medicine formula can play the role of antibiosis; other chemical preservatives or bacteriostatic agents are not needed in the production process of the product. Can also greatly reduce the toxicological effect of the product, and simultaneously has the bacteriostatic effect to prevent infection in the using process.

Meanwhile, the product process adopts an extraction technology for purifying the effective components. In addition, the unique traditional Chinese medicine extract is embedded, the embedding liquid is dispersed, and the traditional Chinese medicine extract is filled in the mixture at the medium temperature, so that the stability is realized at the low temperature. Can make the product more fully and stably embedded, reduce the loss of effective components and prolong the service life of the product, and compared with the common traditional Chinese medicine extraction preparation, the method can prolong the effective period by about 1.5 years.

The efficacy of the herbal extract; the extract adopts honeysuckle as a monarch drug to play a role in clearing away heat and toxic materials and removing bacteria, and the rhubarb and the angelica are ministerial drugs to play a role in promoting blood circulation and removing meridian obstruction as well as resisting bacteria and diminishing inflammation; the dark plum is used as an adjuvant to play a role in astringing and stopping bleeding. The combined use of the monarch, the minister, the assistant and the guide of the traditional Chinese medicine can reduce the stimulation and sensitization of the product and accelerate the repair of the damaged part.

The hydroxyethyl cellulose has good film forming property, the dispersion liquid of the aqueous solution of the hydroxyethyl cellulose can form a stable three-dimensional network structure, the active ingredients of the traditional Chinese medicine extract can be embedded, and meanwhile, the hydroxyethyl cellulose has good lubricity.

The polyvinylpyrrolidone (preferably polyvinylpyrrolidone K30 in the scheme) has good affinity and compatibility with human body, and can be used as a carrier of the traditional Chinese medicine extract together with the hydroxyethyl fiber, so that the traditional Chinese medicine extract can be promoted to contact and absorb with skin, and the traditional Chinese medicine extract can quickly exert the drug effect to play the roles of reducing irritation and allergy and promoting the repair of damaged parts.

In addition, the screen meshes used in the steps S3 and S4 are 280-320 meshes.

Furthermore, the mesh number of the screen is required to be controlled within 280 meshes and 320 meshes, otherwise, the product cannot be matched with the viscosity of the product to form a shaped dilute liquid.

the preparation method of the embedding liquid is the preparation method of the embedding liquid;

the mixing method of the transparent thin gel comprises the following steps:

the environment of the reaction kettle of the carbomer dispersion is 12Mpa, and the carbomer dispersion is uniformly dispersed at 15000 r/min and 120 ℃ for 5min, so that carbomer molecules can be crushed and refined in a short time, the carbomer molecules are uniformly mixed with glycerol, and triethanolamine is added to form the gel with ultrahigh delicate feeling. The reduction to 40 deg.C is intended to reduce the carbomer gel to a suitable temperature for convenient mixing with the embedding solution of the herbal extract. The carbomer gel is added to a certain amount, so that the viscosity of the carbomer gel is increased, the carbomer gel is a thin liquid with a certain excipient, the carbomer gel is convenient to use and easy to apply, and is fresh and non-sticky when used. The high-temperature high-pressure high-speed homogeneous carbomer is more uniform and fine when used, the original effect of the lubricity of the product is improved, and meanwhile, the absorption of the effective components of the traditional Chinese medicine is promoted, and the effect of the medicine is further promoted.

Still further illustratively, the reaction processor comprises: an ultrasonic device 43; the ultrasonic device 43 is installed in the outer reaction vessel 1. The purpose of ultrasonic vibration is to further increase the stability and uniformity of the product and simultaneously remove air bubbles caused during the filling process of the product.

the kettle-in-kettle structure of the reaction kettle comprises: an outer reaction kettle 1, an inner reaction kettle 2, an inner kettle feeder 3 and a reaction processor;

at least two inner reaction kettles 2 are arranged in the outer reaction kettle 1; the inner reaction kettle 2 is provided with an inner kettle feeding hole 21 and an inner kettle discharging hole 22, and the inner kettle discharging hole 22 is positioned at the bottom of the inner reaction kettle 2; the inner kettle feeder 3 is arranged at the inner kettle feed inlet 21 and communicated with the reaction cavity of the inner reaction kettle 2 and the outside of the outer reaction kettle 1, and the inner kettle discharge outlet 22 is communicated with the reaction cavity of the inner reaction kettle 2 and the reaction cavity of the outer reaction kettle 1; the inner kettle feeder 3 and the inner kettle discharge port 22 are respectively provided with an inner kettle switch 23; the reaction processor is arranged in the outer reaction kettle 1 and/or the inner reaction kettle 2 and is used for adjusting the reaction environment of the outer reaction kettle 1 and/or the inner reaction kettle 2.

Stated further, the internal tank feeder 3 includes: a feeding shell 32, a feeding screw rod 33, a motor mounting seat 31 and a feeding motor 34; the output end of the feeding shell 32 is communicated with the feeding hole 21 of the inner kettle, and the feeding screw rod 33 is arranged in the feeding shell 32; the motor mounting seat 31 is mounted on the outer reaction kettle 1; the feeding motor 34 is mounted on the motor mounting seat 31, and the feeding motor 34 is used for driving the feeding screw rod 33 to rotate. A feeding screw rod 33 is arranged in the feeding shell 32, when the feeding motor 34 is started, the feeding motor 34 drives the feeding screw rod 33 to rotate, materials can be added at the input end of the feeding shell 32 at the moment, and the materials can enter the inner reaction kettle 2 from the output end of the feeding shell 32 along the feeding shell 32 under the action of the feeding screw rod 33; the feeding screw rod 33 rotates to feed the material to the lower part and provides a shearing force to prevent the material from being agglomerated and fed to the inner wall of the feeding shell 32 and well disperse the material; after the materials are input, the inner kettle switch 23 and the feeding motor 34 are only required to be closed; here, one end of the feeding screw 33 may be rotatably mounted on the motor mounting seat 31 through a bearing, so that the feeding screw 33 is fixed in position and stability is improved.

Further illustratively, the reaction processor comprises: a high-temperature heater 41;

the heating end of the high-temperature heater is in contact with the outer reaction kettle 1 and/or the inner reaction kettle 2, and the high-temperature heater is used for heating the inner part of the outer reaction kettle and/or the inner reaction kettle.

Further illustratively, the reaction processor comprises: a cryogenic liquid flow channel 9;

Further illustratively, the reaction processor comprises: a high speed wall breaking device 44;

the high-speed wall breaking device 44 comprises: a wall breaking driver 441 and a wall breaking cutter 442;

the fixed end of the wall-breaking driver 441 is installed in the outer reaction kettle 1 and/or the inner reaction kettle 2, and the wall-breaking driver 441 is used for driving the wall-breaking cutter 442 to rotate.

To be more specific, the wall-breaking driver 441 can drive the wall-breaking cutter 442 to rotate, so that the materials at the bottom of the outer reaction vessel 1 and/or the bottom of the inner reaction vessel 2 can be dispersed at a high speed, the mixing degree can be improved, and the materials can be prevented from being coagulated into blocks. In particular, in the case of a chain extension reaction of a polymer, if the shearing force for stirring is insufficient, the polymer is likely to form a lump, which affects the reaction.

Further, the method further comprises the following steps: an outer kettle feeder 7;

the outer reaction kettle 1 is provided with an outer kettle feeding hole 11 and an outer kettle discharging hole 12; the outer kettle feeder 7 is arranged at the outer kettle feeding hole 11; the outer kettle feeder 7 and the outer kettle discharge port 12 are respectively provided with an outer kettle switch 13.

S1, adding 9 times of water into 1 part of honeysuckle and 1 part of angelica, and obtaining aromatic water a by a steam distillation method;

s2, mixing the residue obtained after filtration in the step S1 with 1 part of rhubarb and 1 part of dark plum, adding 9 times of water in the mixing amount, and cooking for 60 min;

s3, filtering with a 300-mesh screen to obtain filtrate b and dregs, and decocting the dregs in 5 times of water for 30 min; filtering with a 300-mesh screen to obtain filtrate c;

s4, combining the filtrate b and the filtrate c to obtain a total filtrate, precipitating the total filtrate by 60 percent of absolute ethyl alcohol and filtering the precipitate by a 300-mesh screen; obtaining final filtrate c;

s5, stirring at 300r/min, slowly adding aromatic water a into the final filtrate c to obtain Chinese medicinal extract X, sealing at 5 + -2 deg.C, and storing;

s6, dissolving 0.15% hydroxyethyl fiber with 10 ten thousand viscosity and 0.35% polyvinylpyrrolidone K30 in water, keeping 10Mpa in a reaction kettle A in a kettle-in-kettle structure, and keeping 120 ℃ for 3min at 12000 r/min;

s7, injecting low-temperature circulating cooling water into a low-temperature liquid flow channel of the inner reaction kettle A, and cooling to the temperature of a detection point of 40 ℃ to enable the dispersion liquid to be in a uniform state;

s8, injecting 25% of Chinese medicinal extract X into the inner reaction kettle A, and stirring the dispersion in a stirring tank at a stirring speed of 3000 r/min under a pressure of 10MPa at 40 deg.C, and reversing every 5min for 30 min;

s9, after stirring is stopped, high-pressure low-temperature circulating cooling water is adopted in the interlayer, and the interlayer is cooled to the probing point temperature of 5 ℃ and sealed for 1 h; the Chinese medicine extract is fully and stably filled in the three-dimensional grid of the high polymer material; obtaining an embedding solution of the traditional Chinese medicine extract;

s10, putting 20% glycerol, 0.2% carbomer and water into an inner reaction kettle B while embedding important extracts, keeping the pressure at 12Mpa, and keeping the temperature at 15000 r/min for 5min at 120 ℃ for homogenizing and dispersing;

s11, adding triethanolamine at the same pressure and temperature, and stirring at 2000r/min for 5min to obtain transparent dilute gel; then keeping the pressure, injecting high-pressure low-temperature circulating cooling water into the interlayer, and cooling the interlayer to a detection temperature of 40 ℃;

s12, allowing the prepared traditional Chinese medicine extract embedding liquid in the inner reaction kettle A and the prepared transparent dilute gel in the inner reaction kettle B to enter the outer reaction kettle through the inner kettle switch by pressure difference; stirring at 1300r/min and normal temperature of 10Mpa under vacuum state and adding the rest water; stirring for 30min, filling and sealing to obtain water-soluble human body lubricating liquid, and ultrasonically shaking for 2min to obtain the final water-soluble human body lubricating liquid product.

Inoculation culture and antibacterial experiment of Escherichia coli

Preparation of the culture Medium

The main antibiosis of the experiment is to adopt anti-colibacillus, then firstly, the culture medium is taken, the used culture medium is beef extract peptone solid culture medium, and the components are as follows: 3g of beef extract, 10g of peptone, 5g of NaCl, 18g of agar, 1000mL of water and pH 7.4-7.6.

The method comprises the following specific configuration steps:

1. medicine weighing device

First, 15g of sodium chloride was weighed and poured into a 1000ml large beaker. And weighing beef extract which is paste with high adhesiveness, putting the beef extract in a watch glass, weighing, dissolving the beef extract in 10g of hot water prepared in advance, and pouring the beef extract into a large beaker containing sodium chloride. Peptone is a pale yellow meat meal which is very hygroscopic and needs to be weighed quickly, and then poured into the large beaker. Agar 18g was weighed and placed in a small beaker until use. The preparation work is completed.

2. Dissolving by heating

Pouring a small amount of water into the prepared beaker, placing the beaker on an asbestos net, heating the beaker with soft fire, stirring the beaker with a glass rod, supplementing the water to 1000ml after the medicine is completely dissolved, then placing the prepared agar into a large beaker, continuously heating and melting the agar, continuously stirring the agar with the glass rod during the process to prevent the agar from being burnt or overflowing, and finally supplementing the missing water.

3. Adjusting the pH

The pH value of the culture medium is detected, the pH value of the culture medium prepared by our team is 6.7, 1mol/l NaOH needs to be added, 10 drops are added in total, and 1mol/l HCl 3 drops are added in a dropwise manner, so that the pH value is 7.4.

4. Making tampons

The test tube is made of common cotton and made of cotton wool. The shape, size and tightness of the cotton plug are proper, the periphery of the cotton plug is tightly attached to the pipe wall, and the cotton plug can play a role in preventing the invasion of mixed bacteria and facilitating ventilation without gaps. The tampon may be inserted into the test tube orifice or neck opening for an overall length of about 3/5 to prevent the tampon from falling out.

5. Dispensing

The prepared culture medium is divided into 5 test tubes. The funnel can be used for subpackaging so as to prevent the culture medium from being stained on the pipe orifice or the bottle mouth to cause pollution.

The subpackage amount is that the beef peptone medium is about l/5 of the height of the test tube.

6. Tampon application

The prepared cotton plugs are respectively put on test tubes filled with culture medium.

7. Wrapping up

Because the culture medium is separately packed in the test tubes, 5 test tubes are put together, and then a layer of kraft paper is coated outside the cotton plug and tied by a rope. The medium name, group, date are then noted with a marker.

8. Sterilization

And (3) placing the labeled and bound test tubes into a steam type pressure cooker for sterilization, wherein the temperature can generally reach about 160 ℃, and the sterilization time is 2 hours.

9. Preservation of

And (5) placing the sterilized test tubes into a refrigerator for storage.

Inoculation and culture of Escherichia coli

The entire process of inoculation is completed on a sterile operating table.

1. Placing the sterilized test tube culture medium to be used and the culture medium with formed bacterial colony on a sterile experiment operating platform, and then igniting an alcohol lamp to burn the test tube culture medium and the culture medium.

2. The hands are wiped by alcohol, and the test bed is also wiped clean, so that the possible bacteria are removed, and the real sterile operation is realized.

3. The inoculating loop was burned on an alcohol burner until the inoculating loop burned red.

4. The bacterial strain test tube with grown bacterial colony is set beside the alcohol lamp, the piston is turned on, the alcohol lamp is burnt for some time, the inoculating loop is used to take out some bacterial strain from the culture medium, and the piston is plugged beside the alcohol lamp and set aside.

5. The prepared culture medium to be used is placed beside an alcohol lamp, a cotton plug is opened, an inoculating loop is placed in the prepared culture medium to inoculate the strain by a streaking method, and then the cotton plug is placed aside beside the alcohol lamp.

6. Repeating the steps, and completely inoculating 5 test tubes.

7. Finally, 5 inoculated test tubes are wrapped by newspaper again and cultured in an incubator at 37 ℃ for 3 days.

Second, antibacterial experiment

1. The medium was poured into a triangular flask, 150 ml. 100ml of water was then poured into the flask.

2. The above medium, ten empty petri dishes and a triangular flask containing 100ml of water were sterilized in an autoclave medium for 2 hours.

3. Cutting cotton fabric dyed with Chinese herbal medicine extract into small blocks with diameter of 1.5cm, placing in a culture dish, and sterilizing in a drying oven at 95 deg.C for 2 hr.

4. And uniformly pouring the culture medium in the sterilized culture medium in the triangular flask into ten sterilized culture dishes, and storing in a refrigerator.

5. The strain was inoculated on a sterile table in the center of the petri dish and streaked.

6-1, dripping a little sterile water on the cotton fabric sterilized before, and then using sterilized tweezers to spread the cotton fabric in the oven on two sides of the inoculated culture dish in parallel. And repeating the steps, after all the cotton fabrics are paved, putting all the culture dishes into an incubator with constant temperature of 37 ℃ for 3 days, and observing the growth condition of the cotton fabrics.

The extracting solution adsorbed by the fabric slowly diffuses to the periphery, so that a clear bacteriostatic zone appears around the fabric. The size of the inhibition zone represents the antibacterial capacity of the extracting solution, and under the condition of different medicament components of the same strain, the larger the inhibition zone is, the antibacterial capacity is improved. The inhibition range of the inhibition zone is expressed by the diameter.

6-2, dipping the extracting solution, coating the extracting solution on culture dishes to form an equal-size inhibition zone, putting all the culture dishes in an incubator with a constant temperature of 37 ℃ for culturing for 3 days, and observing the growth condition of strains in the inhibition zone; the time required for the appearance of the strain in the circle was recorded.

Example A:

s1, adding 9 times of water into the honeysuckle and the angelica to obtain aromatic water a by a steam distillation method; the addition of honeysuckle and angelica is shown in table 1;

s2, mixing the residue obtained after filtration in the step S1 with rhubarb and dark plum, adding 9 times of water, and cooking for 60min, wherein the addition condition of rhubarb and dark plum is shown in Table 1;

s6, dissolving 0.15% hydroxyethyl fiber with 10 ten thousand viscosity and 0.35% polyvinylpyrrolidone K30 in water, keeping 10Mpa, and keeping 120 ℃ for 3min at 12000 r/min;

s7, controlling the reaction temperature of the hydroxyethyl fibers and polyvinylpyrrolidone K30 at 40 ℃, and enabling the dispersion liquid to be in a uniform state;

s8, placing 25% of the Chinese medicinal extract X into the dispersion liquid obtained in the step S7, stirring at 3000 r/min under 10MPa at 40 deg.C, and reversing every 5min for 30 min;

s9, cooling to the probing point temperature of 5 ℃ after stirring is stopped, and sealing and keeping for 1h under the environment of 10 Mpa; obtaining an embedding solution of the traditional Chinese medicine extract;

s10, mixing 20% glycerol and 0.2% carbomer with water, keeping at 12Mpa, and keeping at 15000 r/min and 120 deg.C for 5min for homogenizing and dispersing;

s11, adding triethanolamine at the same pressure and temperature, and stirring at 2000r/min for 5min until the mixture is bright; then keeping the pressure, cooling to the detection temperature of 40 ℃ to obtain transparent thin gel;

s12, mixing the Chinese medicine extract embedding liquid prepared in the step S9 and the transparent dilute gel prepared in the step S11; stirring at 1300r/min at normal temperature and 10Mpa under vacuum, and adding water; stirring for 30min, filling and sealing to obtain water-soluble human body lubricating liquid, and ultrasonic oscillating for 2min to obtain water-soluble human body lubricating liquid finished product.

TABLE 1 Effect of different Chinese herbs on human body lubricating fluids

6-1 step of an antibacterial experiment is carried out on the table 1, and the influence of each component on the size of a bacteriostatic zone is recorded, as shown in table 2;

TABLE 2 influence of Chinese herbs on the size of zone of inhibition

Description of the drawings:

1. comparing example a2 with example a 1; example A2 showed 1 more parts of rhubarb than example A1, whereas example A2 showed a 0.2cm larger zone diameter, 28.6% more than example A1; the rhubarb is mainly explained as a ministerial drug, can promote the heat-clearing and detoxifying effects of the honeysuckle, and has the functions of promoting blood circulation, removing obstruction in channels, resisting bacteria and diminishing inflammation, thereby improving the final antibacterial effect of the product.

2. Comparing example A3 with example a 1; example A3 shows 1 more part of Dang Gui than example A1, while example A3 shows 28.6 more parts than example A1 with a zone of inhibition 0.2cm in diameter; it shows that the final antibacterial effect of the product is improved after the Chinese angelica is added on the basis of the honeysuckle.

3. Comparative examples a4, A3 and a 2; example A4 use radix Et rhizoma Rhei and radix Angelicae sinensis together with flos Lonicerae; whereas example A2 used only a single rhubarb, example A3 used only a single angelica. Through the experiment, after the rhubarb and the angelica are used simultaneously in the example A4, the bacteriostatic effect is better than that of the examples A3 and A2, specifically, the diameter of the bacteriostatic circle is 1.2cm larger than that of any two of the examples A3 and A2, which shows that when the honeysuckle, the rhubarb and the angelica are used simultaneously, the effect is better than that of any one of the examples A3 and A2, and the three have synergistic effect.

4. Comparative examples a1 and a 5; example a5 has 1 more angelica than example a1, but in terms of performance, the diameter of the zone of inhibition is not increased, i.e. there is no promoting effect on the inhibition, which indicates that adding a single angelica to the honeysuckle does not promote the inhibition effect of the product;

examples a4, a5 and a 6; after 1 part of angelica is added into the example A4, the example A6 is obtained, the bacteriostatic zone of the example A6 is close to the diameter of cotton fabric and is 1.5cm, and the diameter of the example A is larger than that of any example of the examples A1-A5, namely the example A6 is explained to have the best bacteriostatic effect, so that the bacteriostatic effect is the best after the honeysuckle, the rhubarb, the angelica and the dark plum are used in combination, and the bacteriostatic effect is better than that of a monarch drug, a ministerial drug or an assistant and guide drug which are used alone.

Example B

Example B1

Step S1-S5, the traditional Chinese medicine extract X is not needed, so the step is omitted;

s8, adding 25% distilled water into the dispersion liquid obtained in the step S7, stirring at 3000 r/min under 10MPa at 40 ℃, reversing every 5min, and continuously stirring for 30 min;

s9, cooling to the probing point temperature of 5 ℃ after stirring is stopped, and sealing and keeping for 1h under the environment of 10 Mpa; the embedding solution of example B1 was obtained;

s12, mixing the distilled water embedding liquid prepared in the step S9 and the transparent dilute gel prepared in the step S11; stirring at 1300r/min at normal temperature and 10Mpa under vacuum, and adding water; stirring for 30min, filling and sealing to obtain water-soluble human body lubricating liquid, and performing ultrasonic oscillation for 2min to obtain the human body lubricating liquid finished product of the embodiment B1.

Example B2

s6, dissolving polyvinylpyrrolidone K30 (0.15% + 0.35%) in water, keeping 10Mpa, and keeping 120 ℃ for 3min at 12000 r/min;

s7, controlling the container temperature of polyvinylpyrrolidone K30 at 40 ℃ and enabling the dispersion liquid to be in a uniform state;

s9, cooling to the probing point temperature of 5 ℃ after stirring is stopped, and sealing and keeping for 1h under the environment of 10 Mpa; to obtain a semi-encapsulated solution of the herbal extract of example B2;

s10, mixing 20% glycerol, 0.2% carbomer and water, keeping at 12Mpa, stirring at 15000 r/min, and keeping at 120 deg.C for 5min for homogenizing and dispersing;

s12, mixing the semi-embedding liquid of the traditional Chinese medicine extract prepared in the step S9 with the transparent dilute gel prepared in the step S11; stirring at 1300r/min at normal temperature and 10Mpa under vacuum, and adding water; stirring for 30min, filling and sealing to obtain water-soluble human body lubricating liquid, and performing ultrasonic oscillation for 2min to obtain the human body lubricating liquid finished product of the embodiment B2.

Example B3

s12, mixing the Chinese medicine extract embedding liquid prepared in the step S9 and the transparent dilute gel prepared in the step S11; stirring at 1300r/min at normal temperature and 10Mpa under vacuum, and adding water; stirring for 30min, filling and sealing to obtain water-soluble human body lubricating liquid, and performing ultrasonic oscillation for 2min to obtain the water-soluble human body lubricating liquid finished product of the embodiment B3.

Based on the effects of the different processes of the above examples B1-B3, the 6-1 step and the 6-2 step of the above antibacterial test were performed, and the effects on the zone of inhibition between the different steps were recorded. As in table 3.

TABLE 3 influence of examples B1-B3 on the zone of inhibition

Description of the drawings:

example B1 is not prepared by using the Chinese herbal medicine extract X, which is only the hydroxyethyl fiber and polyvinylpyrrolidone K30 embedded in distilled water, and has no bacteriostatic function, so the bacteriostatic action is low, the bacteriostatic duration is low, and the bacterial strain is considered to be still in the growth process within 7h, namely, the bacteriostatic action of example B1.

Example B2 the diameter of the zone of inhibition is 1.0cm, because of using traditional Chinese medicine extract X; however, since hydroxyethyl fiber is not used, the product has no three-dimensional net structure, and the traditional Chinese medicine extract X cannot be bound well, so that the traditional Chinese medicine extract X cannot be continuously inhibited after the inhibition zone reaches 1.0cm, namely, the strain appears in the inhibition zone after 38 h.

In the embodiment B3, the three-dimensional network structure formed by the hydroxyethyl fibers is utilized to bind the traditional Chinese medicine extract X and lock the position of the traditional Chinese medicine extract X, so that the traditional Chinese medicine extract X has a lasting inhibition effect on the strains, and the strains do not appear in the inhibition zone until 81 hours later; meanwhile, when attention needs to be paid, each hydroxyethyl fiber does not have a locking effect on the traditional Chinese medicine extract X, the molecular structure of the traditional Chinese medicine extract X needs to be considered, and the slow release effect is affected because the traditional Chinese medicine extract cannot be well fixed on the net structure due to the structural difference between the net structure and the traditional Chinese medicine extract; the scheme can stabilize the traditional Chinese medicine extract X on the net structure of the hydroxyethyl fiber, so that the traditional Chinese medicine extract can be stabilized on the net structure provided by the hydroxyethyl fiber.

Example C:

s6, dissolving 0.50% hydroxyethyl fiber with 10 ten thousand viscosity in water, keeping 10Mpa, and keeping 120 ℃ for 3min at 12000 r/min;

s7, controlling the reaction temperature of the hydroxyethyl fibers at 40 ℃, and enabling the dispersion liquid to be in a uniform state;

Selecting 30 patients with hand abrasion, wherein the patients are 20-30 years old, 18 men and 12 women; the abrasion condition of the patient is different positions on the arm, belongs to mild and moderate abrasion, and is clinically manifested as local red swelling, including sports abrasion, workpiece abrasion, stumbling abrasion and the like;

the 30 patients are averagely and randomly divided into 1 blank group and 2 treatment groups, each group comprises 10 treatment groups, and the treatment groups comprise a treatment group I and a treatment group II;

blank group: only using clear water for cleaning, and treating every 12h of clear water, wherein the patient recovers naturally;

treatment group (i): the composition is applied to affected parts uniformly in example C, and the composition is changed 1 time every 12 h;

treatment group two: the preparation is applied to affected parts uniformly according to example B3, and the dosage is changed 1 time every 12 h.

Counting the treatment effect of the blank groups and the first to the second groups after 7 days; and strictly control the diet, work and rest and activities of the patients during the treatment process; the traditional Chinese medicine and the western medicine cannot be taken, and the physical therapy cannot be accepted; do not allow too strenuous activity.

The design makes a judgment standard grade of treatment effect after 7 days for a patient: obtained as in Table 4

And 3, level: clinical symptoms disappear, pain and red swelling on the affected part are completely dissipated, the activity function is recovered, and no pain is felt by pressing.

And 2, stage: the pain of the affected part is relieved, the affected part is completely scabbed, and the patient can normally move, but the patient feels slight pain when pressed.

Level 1: the pain of the affected part is relieved, the affected part is scabbed, but part of the edge position is red and swollen, and the affected part still feels pain when not pressed.

TABLE 4 Effect of polyvinylpyrrolidone on mar

	Blank group	Treatment group (1)	Treatment group 2
				Standard grade	Level 1	Stage 2	Grade 3

Description of the drawings:

1. the blank group was treated with distilled water alone without drug treatment, which corresponds to natural repair, so after 7 days, the bruising was still in natural repair.

2. Comparing the treatment group I with the treatment group II; in the preparation process of the treatment group, hydroxyethyl fibers and polyvinylpyrrolidone are not matched to prepare an embedding solution, the treatment effect is only grade 2, and slight pain is still caused after 7 days and when the patient is pressed; in the second treatment group, hydroxyethyl fibers and polyvinylpyrrolidone are matched to prepare embedding liquid, the standard grade of the embedding liquid is 3 grade, the affected part can be completely repaired within 7 days, and the effect is better than that of the first treatment group.

Compared with the treatment group I, the treatment group II has good affinity and compatibility with the human body due to the polyvinylpyrrolidone, can promote the contact and absorption of the traditional Chinese medicine extract and the skin, and enables the traditional Chinese medicine extract to quickly exert the drug effect to play the roles of reducing irritability and promoting the repair of the damaged part.

Example D:

s3, filtering with a 300-mesh screen to obtain filtrate b and dregs, and decocting the dregs in 5 times of water for 30 min; filtering with a 280-mesh screen to obtain filtrate c;

s4, combining the filtrate b and the filtrate c to obtain a total filtrate, precipitating the total filtrate by 60 percent of absolute ethyl alcohol and filtering the precipitate by a 320-mesh screen; obtaining final filtrate c;

s6, dissolving 0.15% hydroxyethyl fiber with 13 ten thousand viscosity and 0.35% polyvinylpyrrolidone K30 in water, keeping 10Mpa, and keeping 120 ℃ for 3min at 12000 r/min;

The technical principle of the present invention is described above in connection with specific embodiments. The description is made for the purpose of illustrating the principles of the invention and should not be construed in any way as limiting the scope of the invention. Based on the explanations herein, those skilled in the art will be able to conceive of other embodiments of the present invention without inventive effort, which would fall within the scope of the present invention.

Claims

1. The application of the embedding liquid in preparing the water-soluble human body lubricating liquid is characterized in that the preparation method of the embedding liquid comprises the following steps:

s9, cooling to a probing point temperature below 10 ℃ after stirring is stopped, and hermetically storing in a high-pressure environment to obtain an embedding solution of the traditional Chinese medicine extract;

the mixing method of the transparent thin gel comprises the following steps:

2. The use of the embedding fluid in the preparation of water-soluble human body lubricant according to claim 1, wherein the step S1 is specifically to obtain aromatic water a by steam distillation with 1 part of honeysuckle and 1 part of angelica sinensis added with 7-11 times of water in the mixing amount;

3. The use of an embedding fluid in the preparation of water-soluble human body lubricant according to claim 1, wherein the mesh number of the screen used in the steps S3 and S4 is 280-320 meshes.

4. Use of an embedding fluid according to claim 1 for the preparation of a water-soluble human body lubricant comprising: 10-40% of traditional Chinese medicine extract X, 0.1-0.2% of hydroxyethyl fiber, 0.3-0.5% of polyvinylpyrrolidone, 0.10-0.15% of carbomer, 0.1-0.2% of triethanolamine, 15-25% of glycerol and the balance of water.

5. The use of an embedding fluid in the preparation of water-soluble human body lubricating fluid according to claim 1, wherein the step S12 is specifically to mix the Chinese medicinal extract embedding fluid prepared in step S9 with the clear thin gel prepared in step S11; stirring and adding water under high pressure, normal temperature and vacuum state; stirring and then ultrasonically oscillating to obtain the water-soluble human body lubricating liquid.

6. The use of an embedding fluid in the preparation of a water-soluble human body lubricating fluid according to claim 1, wherein the embedding fluid is prepared by a kettle-in-kettle structure in a reaction kettle;

7. The use of an embedding fluid in the preparation of a water-soluble human body lubricant according to claim 6, wherein the reaction processor comprises: a high temperature heater;

8. The use of an embedding fluid in the preparation of a water-soluble human body lubricant according to claim 6, wherein the reaction processor comprises: a cryogenic liquid flow channel;

9. The use of an embedding fluid in the preparation of a water-soluble human body lubricant according to claim 6, wherein the reaction processor comprises: a high-speed wall breaking device;