CN110308549A - A kind of whole slide multi-pass high throughput pathological section scanner - Google Patents
A kind of whole slide multi-pass high throughput pathological section scanner Download PDFInfo
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- 230000001575 pathological effect Effects 0.000 title claims abstract description 23
- 230000003287 optical effect Effects 0.000 claims abstract description 85
- 238000003384 imaging method Methods 0.000 claims abstract description 60
- 229910052736 halogen Inorganic materials 0.000 claims abstract description 53
- 229910052721 tungsten Inorganic materials 0.000 claims abstract description 53
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- 150000002367 halogens Chemical class 0.000 claims abstract description 52
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000011521 glass Substances 0.000 claims abstract description 42
- 230000007170 pathology Effects 0.000 claims abstract description 14
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
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- 201000010099 disease Diseases 0.000 description 2
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- 238000000701 chemical imaging Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002059 diagnostic imaging Methods 0.000 description 1
- 230000003118 histopathologic effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000010801 machine learning Methods 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
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- G—PHYSICS
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- G02B21/00—Microscopes
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- G—PHYSICS
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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Abstract
The invention discloses a kind of whole slide multi-pass high throughput pathological section scanners, it includes by two sets of halogen tungsten lamp light sources, optically focused microscope group, motorized subject table, object lens, zoom lens, the symmetric doublet optical path of corner cube mirror composition, glass slide pallet, magnetic, glass slide, imaging len, filter unit, Double-face rotating reflecting mirror, glass slide pallet entrance, objective table x motor, objective table y motor, LED area array light source, whole slide imaging lens group, whole slide imaging detector, gray scale scanning imaging detector, chromoscan camera, camera connecting bracket, rotate mirror driver, zoom controller, filter unit controller, acquisition and control unit, power supply, system shell, optical path shell and interface.The high-throughput acquisition for realizing pathology sample colour and multiband image data is imaged by double light path simultaneously by the present invention, can provide pathology sample various dimensions image data source.
Description
Technical field
It is detection target that the present invention relates to a kind of pathological section scanning imaging instrument, especially one kind with pathology sample, is carried out
The scanner of whole slide multi-pass high throughput pathological section imaging.
Background technique
Although various medical imaging technologies are used for the auxiliary diagnosis of disease, group with the development of information technology
Knit " goldstandard " that pathological analysis is still the various diseases diagnosis and guiding treatment including tumour.Traditional pathology is examined
It is disconnected based on the observation of pathologist microscope, there are time-consuming and laborious, the problems such as being easy missing inspection.In recent years as information technology is special
The development of artificial intelligence technology, using the intelligent identification Methods such as machine learning to pathological images carry out automatic identification and diagnosis at
For a kind of new trend.But artificial intelligence technology is applied to Pathologic Diagnosis, it is necessary first to obtain a large amount of pathology sample
Full slide image data.Existing full slide pathological images acquisition system can be scanned into the pathology sample of whole slide
Picture, but when facing great amount of samples, however it remains slow problem, and obtaining is conventional color image, it is more difficult to it is real
The Rapid Accumulation of existing big data quantity.Therefore design a kind of new full slide multi-pass high throughput pathological section image scanning instrument for
Realize that the intelligence of pathological diagnosis has great importance.
Summary of the invention
A kind of full slide multi-pass high pass provided the purpose of the present invention is the deficiency for existing pathology imaging technique
Measure pathological section scanner.The pathological section scanner is carried out simultaneously using different zones of multiple object lens to same pathology slide
Colored and multi-spectral imaging, plans scanning mode according to the profile of whole slide, to improve whole pathology slide colour automatically
With the scanning speed of multiband image data, a large amount of pathological section various dimensions image data is obtained, improves pathological section multidimensional
The automation and intelligence of imaging are spent, the workload of data acquisition is reduced, is quickly provided for artificial intelligent recognition analysis and diagnosis
Mass data source, to assist pathologist to provide more accurate pathological diagnosis as a result, mitigating pathologist burden.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of whole slide multi-pass high throughput pathological section scanner, it include the first halogen tungsten lamp light source, the second halogen tungsten lamp light source,
First optically focused microscope group, second condenser lens group, motorized subject table, the first object lens, the second object lens, the first zoom lens, the second zoom
Lens, the first corner cube mirror, the second corner cube mirror, glass slide pallet, magnetic, glass slide, imaging len, filter unit,
Double-face rotating reflecting mirror, glass slide pallet entrance, objective table x motor, objective table y motor, LED area array light source, the imaging of whole slide
Lens group, whole slide imaging detector, gray scale scanning imaging detector, chromoscan camera, camera connecting bracket, revolving mirror drive
Dynamic device, zoom controller, filter unit controller, acquisition and control unit, power supply, system shell, optical path shell, interface, clothes
Business device, database, client, glass slide clamp.First halogen tungsten lamp light source, the second halogen tungsten lamp light source are placed under motorized subject table
End, is fixed on system shell pedestal, and the first halogen tungsten lamp light source front end is equipped with the first optically focused microscope group, the second halogen tungsten lamp light source front end
Equipped with second condenser lens group；Optical path shell lower end is equipped with the first object lens, the second object lens, is terminated with the first zoom after the first object lens
Lens are terminated with the first corner cube mirror after the first zoom lens, the second zoom lens, the second zoom are terminated with after the second object lens
Lens rear is connected to the second corner cube mirror；First object lens, the second object lens, the first zoom lens, the second zoom lens, first are directly
Corner reflector, the second corner cube mirror are symmetrically fixed on optical path shell, symmetry axis and the first corner cube mirror, the second right angle
Reflecting mirror intersection point middle position is equipped with Double-face rotating reflecting mirror；Double-face rotating reflecting mirror upper end is equipped with filter unit, filters
There is imaging len in unit upper end, and imaging len upper end is equipped with gray scale scanning imaging detector；Double-face rotating reflecting mirror lower end peace
Equipped with chromoscan camera；The LED area array light source is placed in motorized subject table lower end, is fixed on system shell pedestal, is located at
First halogen tungsten lamp light source side；Whole slide imaging lens group is fixed on optical path shell by camera connecting bracket, whole slide at
As being terminated with whole slide imaging detector in lens group；Motorized subject table is placed in the first object lens, the second object lens and the first tungsten halogen lamp
Between light source, the second halogen tungsten lamp light source, motorized subject table edge is equipped with objective table x motor, objective table y motor, motorized subject table
Quadrangle is equipped with magnetic, and glass slide pallet is placed on magnetic, and glass slide is placed on glass slide pallet and is fixed by glass slide clamp, is
Glass slide pallet entrance is provided on system shell；System shell bottom is fixed with power supply, and power supply output is connected respectively to LED area array light
The power input in source, the first halogen tungsten lamp light source power input, the second halogen tungsten lamp light source power input, acquisition and control are single
The power input of member；Acquisition and control unit are fixed on system shell, and the stage motor of acquisition and control unit controls
Output end is connected respectively to the control signal of objective table x motor, objective table y motor；The revolving mirror control of acquisition and control unit
Output end processed is connected to the control signal of rotation mirror driver, and the control output end for rotating mirror driver is connected to Double-face rotating
In the control signal of reflecting mirror；The Zoom control output end of acquisition and control unit is connected to the control input of zoom controller
End, the control output end of zoom controller is connected respectively to the control signal of the first zoom lens, the second zoom lens；Acquisition
The control signal of filter unit controller is connected to the control output end of control unit, the control of filter unit controller is defeated
Outlet is connected to the control signal of filter unit；The output end of whole slide imaging detector is connected to acquisition and control unit
Data input pin；The output end of gray scale scanning imaging detector is connected to the data input pin of acquisition and control unit；Colour is swept
The output end for retouching camera is connected to the data input pin of acquisition and control unit；The input/output signal line of acquisition and control unit
It is connected on the interface of system shell；Scanner is in use, server can be connected on the interface of system shell, server
Equipped with database, client is connected on server.
The filter unit can be optical filter runner, prism, grating, liquid crystal tunable filter or the adjustable optical filtering of acousto-optic
Device.
First halogen tungsten lamp light source, the first optically focused microscope group, the first object lens, the first zoom lens, the first corner cube mirror
Form the first optical path, the second halogen tungsten lamp light source, second condenser lens group, the second object lens, the second zoom lens, the second corner cube mirror
The second optical path is formed, two-way optical path can exchange direction by Double-face rotating reflecting mirror.
Two first optical path, the second optical path optical paths can also increase to four optical paths.
The scanner can delimit two scanning areas automatically according to sample areas aspect ratio on glass slide and select the side x
To or one of two kinds of scanning modes in the direction y, to improve the efficiency of scanning.
The present invention realizes the automatic whole slide multi-mode high throughput scanning of histopathologic slide, can obtain pathology simultaneously
The multiband image data and full color data of tissue.In data acquisition, obtained first using whole slide imaging detector
The image data of whole slide is divided into two of the direction x or the direction y according to the chamfered shape of pathology sample in slide
Sample is moved under objective table by region, acquisition and control unit control motorized subject table, two the first object lens, the second object lens objects
Mirror respectively corresponds the initial position in two regions, and acquisition and control unit control the first zoom lens, the second zoom lens carry out
Imaging samples focusing, the color image of the current field of the first optical path is acquired by chromoscan camera；Acquisition and control unit
Control control filter unit filter to the second optical path and acquires single band image using gray scale scanning imaging detector, carries out
It is continuous to filter and obtain multiband sample image data after acquiring；Acquisition and control unit control Double-face rotating are anti-after the completion of acquisition
It penetrates mirror to be rotated by 90 °, two-way optical path is exchanged, the color image of the current field of the second optical path is acquired by chromoscan camera,
The multiband sample image data of the first optical path is acquired by filter unit and gray scale scanning imaging detector；Acquisition and control are single
Sample is moved to next visual field along the direction x or the direction y and carries out repeating the above step by first further control motorized subject table
Rapid acquisition, it can the pathology sample image data of quick obtaining multiband and full color are simultaneously deposited into server, client
End with access server downloading data and can carry out the diagnostic analysis of pathological images data.
Detailed description of the invention
Fig. 1 is structure of the invention block diagram；
Fig. 2 is that inventive sample acquisition optical path switches schematic diagram；
Fig. 3 is connection system use state diagram of the present invention；
Fig. 4 is sample of the present invention x scan pattern schematic diagram；
Fig. 5 is sample of the present invention y scan pattern schematic diagram.
Specific embodiment
With reference to the accompanying drawings and embodiments, illustrate structure feature of the invention, technical performance and effect.
Refering to fig. 1-5, the present invention includes the first halogen tungsten lamp light source 101, the second halogen tungsten lamp light source 201, the first optically focused microscope group
102, second condenser lens group 202, motorized subject table 3, the first object lens 103, the second object lens 203, the first zoom lens 104, second
Zoom lens 204, the first corner cube mirror 105, the second corner cube mirror 205, glass slide pallet 4, magnetic 5, glass slide 6, at
As lens 701, filter unit 702, Double-face rotating reflecting mirror 8, glass slide pallet entrance 9, objective table x motor 10, objective table y electricity
Machine 11, LED area array light source 12, whole slide imaging lens group 13, whole slide imaging detector 14, gray scale scanning imaging detector
15, chromoscan camera 16, camera connecting bracket 17, rotation mirror driver 18, zoom controller 19, filter unit controller
20, acquisition and control unit 21, power supply 22, system shell 23, optical path shell 24, interface 25, server 26, database 27, visitor
Family end 28, glass slide clamp 29.First halogen tungsten lamp light source 101, the second halogen tungsten lamp light source 201 are placed in 3 lower end of motorized subject table, Gu
It is scheduled on 23 pedestal of system shell, 101 front end of the first halogen tungsten lamp light source is equipped with the first optically focused microscope group 102, the second halogen tungsten lamp light source
201 front ends are equipped with second condenser lens group 202；24 lower end of optical path shell is equipped with the first object lens 103, the second object lens 203, the first object
It is terminated with the first zoom lens 104 after mirror 103, the first corner cube mirror 105, the second object are terminated with after the first zoom lens 104
It is terminated with the second zoom lens 204 after mirror 203, the second corner cube mirror 205 is terminated with after the second zoom lens 204；First object
Mirror 103, the second object lens 203, the first zoom lens 104, the second zoom lens 204, the first corner cube mirror 105, the second right angle
Reflecting mirror 205 is symmetrically fixed on optical path shell 24, is respectively formed the first optical path 106 and the second optical path 107；First optical path 106
It is equipped with the title axis of the second optical path 107 and the first corner cube mirror 105,205 intersection point middle position of the second corner cube mirror double
Face rotating mirror 8, Double-face rotating reflecting mirror 8 realize the exchange to the first optical path and the second optical path direction by rotation；It is two-sided
8 upper end of rotating mirror is equipped with filter unit 702, and there are imaging len 701,701 upper end of imaging len in 702 upper end of filter unit
Gray scale scanning imaging detector 15 is installed；8 lower end of Double-face rotating reflecting mirror is equipped with chromoscan camera 16；The face LED
Array light source 12 is placed in 3 lower end of motorized subject table, is fixed on 23 pedestal of system shell, is located at 101 side of the first halogen tungsten lamp light source；
Whole slide imaging lens group 13 is fixed on optical path shell 24 by camera connecting bracket 17, whole 13 upper end of slide imaging lens group
It is connected to whole slide imaging detector 14；Motorized subject table 3 is placed in the first object lens 103, the second object lens 203 and the first halogen tungsten lamp light source
101, between the second halogen tungsten lamp light source 201,3 edge of motorized subject table is equipped with objective table x motor 10, objective table y motor 11, is used for
Control the movement in the direction objective table x and the direction y；3 four jiaos of motorized subject table be equipped with magnetic 5, glass slide pallet 4 be placed in magnetic 5 it
On, it is fixed in motorized subject table 3；Glass slide 6 is placed on glass slide pallet 4 and is fixed by glass slide clamp 29, outside system
Glass slide pallet entrance 9 is provided on shell 23, for being put into glass slide pallet 4；23 bottom of system shell is fixed with power supply 22, power supply
22 outputs are connected respectively to power input, 101 power input of the first halogen tungsten lamp light source, the second halogen of LED area array light source 12
201 power input of tungsten light source, acquisition and control unit 21 power input；Acquisition and control unit 21 are fixed on system
On shell 23, the stage motor control output end of acquisition and control unit 21 is connected respectively to objective table x motor 10, objective table
The control signal of y motor 11；The revolving mirror control output end of acquisition and control unit 21 is connected to rotation mirror driver 18
The control output end of control signal, rotation mirror driver 18 is connected in the control signal of Double-face rotating reflecting mirror 8；Acquisition
The control signal of zoom controller 19, the control of zoom controller 19 are connected to the Zoom control output end of control unit 21
Output end is connected respectively to the control signal of the first zoom lens 104, the second zoom lens 204；Acquisition and control unit 21
Optical filtering control output end be connected to the control signal of filter unit controller 20, the control output of filter unit controller 20
End is connected to the control signal of filter unit 702；The output end of whole slide imaging detector 14 is connected to acquisition and control is single
The data input pin of member 21；The output end of gray scale scanning imaging detector 15 is connected to acquisition and the data input of control unit 21
End；The output end of chromoscan camera 16 is connected to the data input pin of acquisition and control unit 21；Acquisition and control unit 21
Input/output signal line be connected on the interface 25 of system shell 23；Server 26 is connected to the interface 25 of system shell 23
On, server 26 is equipped with database 27；Client 28 is connected on server 26.
The filter unit 21 can be optical filter runner, prism, grating, liquid crystal tunable filter or the adjustable filter of acousto-optic
Light device.
As shown in Figure 1, Figure 2, first halogen tungsten lamp light source 101, the first optically focused microscope group 102, the first object lens 103, the first zoom
Lens 104, the first corner cube mirror 105 form the first optical path 106, the second halogen tungsten lamp light source 201, second condenser lens group 202, the
Two object lens 203, the second zoom lens 204, the second corner cube mirror 205 form the second optical path 107, the first optical path 106 and second
Optical path 107 carries out optical path exchange by Double-face rotating reflecting mirror 8.
Such as Fig. 4, Fig. 5, the scanner can delimit two scanning areas according to sample areas aspect ratio on glass slide 6 automatically
Domain simultaneously selects the direction x or two kinds of the direction y scanning mode, to improve the efficiency of scanning.
Embodiment
Refering to Fig. 3, in the present embodiment, the first halogen tungsten lamp light source 101, the second halogen tungsten lamp light source 201 are placed in motorized subject table 3
Lower end is fixed on 23 pedestal of system shell, and 101 front end of the first halogen tungsten lamp light source is equipped with the first optically focused microscope group 102, the second halogen tungsten
201 front end of lamp source is equipped with second condenser lens group 202, realizes the spotlighting to pathological section sample；24 lower end of optical path shell
First object lens 103, the second object lens 203 are installed, are terminated with the first zoom lens 104, the first zoom lens after the first object lens 103
It is terminated with the first corner cube mirror 105 after 104, the second zoom lens 204, the second zoom lens are terminated with after the second object lens 203
The second corner cube mirror 205 is terminated with after 204；First object lens 103, the second object lens 203, the first zoom lens 104, the second zoom
Lens 204, the first corner cube mirror 105, the second corner cube mirror 205 are symmetrically fixed on optical path shell 24, are respectively formed
One optical path and the second optical path；The title axis and the first corner cube mirror 105, the second corner cube mirror of first optical path and the second optical path
205 intersection point middle positions are equipped with Double-face rotating reflecting mirror 8, Double-face rotating reflecting mirror 8 realized by rotation to the first optical path and
The exchange of second optical path direction；8 upper end of Double-face rotating reflecting mirror is equipped with filter unit 702, realizes and carries out to the light in optical path
Filtering selects single band light to reach imaging len 701, and there are imaging len 701,701 upper end of imaging len in 702 upper end of filter unit
Gray scale scanning imaging detector 15 is installed, single-range image data is acquired；8 lower end of Double-face rotating reflecting mirror is installed by chromatic colour
Scanning camera 16, for acquiring the color catalog image of another way；The LED area array light source 12 is placed in 3 lower end of motorized subject table,
It is fixed on 23 pedestal of system shell, is located at 101 side of the first halogen tungsten lamp light source, shone when for whole slide to be imaged
It is bright；Whole slide imaging lens group 13 is fixed on optical path shell 24 by camera connecting bracket 17, whole slide imaging lens group 13
On be terminated with whole slide imaging detector 14, for acquiring whole slide image data, identify the profile of sample on slide；It is electronic
Objective table 3 is placed between the first object lens 103, the second object lens 203 and the first halogen tungsten lamp light source 101, the second halogen tungsten lamp light source 201,
3 edge of motorized subject table is equipped with objective table x motor 10, objective table y motor 11, for controlling the shifting in the direction objective table x and the direction y
It is dynamic；3 four jiaos of motorized subject table are equipped with magnetic 5, and glass slide pallet 4 is placed on magnetic 5, is fixed in motorized subject table 3；
Glass slide 6 is placed on glass slide pallet 4 and is fixed by glass slide clamp 29, is provided with glass slide pallet entrance 9 on system shell 23, uses
In being put into glass slide pallet 4；23 bottom of system shell is fixed with power supply 22, and the output of power supply 22 is connected respectively to LED area array light source
12 power input, 101 power input of the first halogen tungsten lamp light source, 201 power input of the second halogen tungsten lamp light source, acquisition and
The power input of control unit 21, for providing power supply to each functional unit；Acquisition and control unit 21 are fixed on system
On shell 23, the stage motor control output end of acquisition and control unit 21 is connected respectively to objective table x motor 10, objective table
The control signal of y motor 11, for controlling objective table in the movement in the direction x and the direction y；The rotation of acquisition and control unit 21
Mirror control output end is connected to the control signal of rotation mirror driver 18, and the control output end of rotation mirror driver 18 is connected to
In the control signal of Double-face rotating reflecting mirror 8, the rotation control of Double-face rotating reflecting mirror 8 is realized；Acquisition and control unit 21
Zoom control output end be connected to the control signal of zoom controller 19, the control output end of zoom controller 19 connects respectively
It is connected to the control signal of the first zoom lens 104, the second zoom lens 204, for realizing the auto-focusing of object lens imaging；It adopts
The optical filtering control output end of collection and control unit 21 is connected to the control signal of filter unit controller 20, filter unit control
The control output end of device 20 is connected to the control signal of filter unit 702, for carrying out single band optical filtering to optical path；Whole slide
The output end of imaging detector 14 is connected to the data input pin of acquisition and control unit 21, for acquiring the image of whole slide
Data；The output end of gray scale scanning imaging detector 15 is connected to the data input pin of acquisition and control unit 21, for acquiring
Single band image data；The output end of chromoscan camera 16 is connected to the data input pin of acquisition and control unit 21, is used for
The full color image data of collecting sample；The input/output signal line of acquisition and control unit 21 is connected to connecing for system shell 23
On mouth 25；Server 26 is connected on the interface 25 of system shell 23, and server 26 is equipped with database 27, is adopted for storing sample
Collect information and image data stores information；Client 28 is connected on server 26, on management and reading service device 26
Image data.
The filter unit 21 can be optical filter runner, prism, grating, liquid crystal tunable filter or the adjustable filter of acousto-optic
Light device.
First halogen tungsten lamp light source 101, the first optically focused microscope group 102, the first object lens 103, the first zoom lens 104,
One corner cube mirror 105 formed the first optical path 106, the second halogen tungsten lamp light source 201, second condenser lens group 202, the second object lens 203,
Second zoom lens 204, the second corner cube mirror 205 form the second optical path 107, and the first optical path 106 and the second optical path 107 pass through
Double-face rotating reflecting mirror 8 carries out optical path exchange.
The scanner can delimit two scanning areas automatically according to sample areas aspect ratio on glass slide 6 and select x
Direction or two kinds of the direction y scanning mode, to improve the efficiency of scanning.
When specifically carrying out pathological section scanning, acquisition and control unit 21 control LED area array light source 12 and light, control electricity
Sample is moved to whole 13 lower section of slide imaging lens group by dynamic object stage 3, acquires whole slide using whole slide imaging detector 14
Image, and identify the edge contour of sample；Acquisition and control unit 21 are determined according to sample chamfered shape uses Fig. 4 or figure
Scanning mode shown in 5；Acquisition and control unit 21 control motorized subject table 3 and sample are moved to the first object lens 103 and second
The initial sweep position in 203 lower two regions of object lens, and LED area array light source 12 is closed, light the first halogen tungsten lamp light source 101,
Two halogen tungsten lamp light sources 201, acquisition and control unit 21 control the first zoom lens 104, the second zoom lens 204 to the two of sample
A optical path is focused respectively, and in the light path imaging of such as Fig. 1, the sample of the first optical path 106 is acquired using chromoscan camera 16
This color image data, acquisition and control unit 21 control filter unit 702 and filter and use gray scale scanning imaging detection
The single band image of 15 the second optical path of continuous acquisition of device, 107 different wave length, finally obtains the multi-band image number of the second optical path 107
According to；Acquisition and control unit 21 control Double-face rotating reflecting mirror 8 and are rotated by 90 °, and the first optical path 106 and the second optical path 107 are switched
For as shown in Fig. 2, acquiring the sample color image data of the second optical path 107 using chromoscan camera 16, acquisition and control are single
Member 21 controls filter unit 702 and filter and using 15 the first optical path of continuous acquisition of gray scale scanning imaging detector, 106 difference
The single band image of wavelength finally obtains the multi-band image data of the first optical path 106；The cromogram of two optical paths is completed in acquisition
After picture and multi-band image, acquisition and control unit 21 control motorized subject table 3 and glass slide 6 are moved to next visual field, weight
The color image and multi-band image data of two optical paths of second mining collection are simultaneously stored into server 26.
Pathology slide is divided into two regions while carrying out color image and multiband by the present invention by double light design
The high-throughput acquisition of image, collection process automatically steps, auto-focusing, can with rapid, high volume obtain the various dimensions of pathological section
Image data provides the support of data for exploitation artificial intelligence pathological diagnosis new algorithm, to improve the effect of Pathologic Diagnosis
Rate and objectivity.
Claims (4)
1. a kind of whole slide multi-pass high throughput pathological section scanner, which is characterized in that slide is divided into pair by the scanner
Claim two regions, carries out the colour and more waves in two regions simultaneously to pathology sample by rotary double-face reflecting mirror using double light path
Section image scanning improves the speed of whole slide imaging；The scanner includes the first halogen tungsten lamp light source (101), the second halogen tungsten light
Source (201), the first optically focused microscope group (102), second condenser lens group (202), motorized subject table (3), the first object lens (103), second
Object lens (203), the first zoom lens (104), the second zoom lens (204), the first corner cube mirror (105), the second right angle are anti-
Penetrate mirror (205), glass slide pallet (4), magnetic (5), glass slide (6), imaging len (701), filter unit (702), two-sided rotation
Turn reflecting mirror (8), glass slide pallet entrance (9), objective table x motor (10), objective table y motor (11), LED area array light source
(12), whole slide imaging lens group (13), whole slide imaging detector (14), gray scale scanning imaging detector (15), colour are swept
Retouch camera (16), camera connecting bracket (17), rotation mirror driver (18), zoom controller (19), filter unit controller
(20), acquisition and control unit (21), power supply (22), system shell (23), optical path shell (24) and interface (25)；
First halogen tungsten lamp light source (101), the first optically focused microscope group (102), the first object lens (103), the first zoom lens
(104), the first corner cube mirror (105) forms the first optical path (106), the second halogen tungsten lamp light source (201), second condenser lens group
(202), the second object lens (203), the second zoom lens (204), the second corner cube mirror (205) form the second optical path (107), and two
Road optical path carries out the exchange in direction by Double-face rotating reflecting mirror (8), realize double light path colour and multi-band image synchronize adopt
Collection；Wherein, the first halogen tungsten lamp light source (101), the second halogen tungsten lamp light source (201) are placed in motorized subject table (3) lower end, are fixed on and are
It unites on shell (23) pedestal, the first halogen tungsten lamp light source (101) front end is equipped with the first optically focused microscope group (102), the second halogen tungsten lamp light source
(201) front end is equipped with second condenser lens group (202)；Optical path shell (24) lower end is equipped with the first object lens (103), the second object lens
(203), it is terminated with the first zoom lens (104) after the first object lens (103), it is straight that first is terminated with after the first zoom lens (104)
Corner reflector (105) is terminated with the second zoom lens (204) after the second object lens (203), and the second zoom lens (204) terminate afterwards
There are the second corner cube mirror (205)；First object lens (103), the second object lens (203), the first zoom lens (104), the second zoom
Lens (204), the first corner cube mirror (105), the second corner cube mirror (205) are symmetrically fixed on optical path shell (24), point
The first optical path (106) and the second optical path (107) are not formed；The symmetry axis and first of first optical path (106) and the second optical path (107)
Corner cube mirror (105), the second corner cube mirror (205) intersection point middle position are equipped with Double-face rotating reflecting mirror (8), two-sided rotation
Turn the exchange of reflecting mirror (8) by rotation realization to the first optical path and the second optical path direction；Double-face rotating reflecting mirror (8) upper end peace
Equipped with filter unit (702), filter unit (702) upper end has imaging len (701), and imaging len (701) upper end is equipped with ash
It spends scanning imagery detector (15)；Double-face rotating reflecting mirror (8) lower end is equipped with chromoscan camera (16)；
The LED area array light source (12) is placed in motorized subject table (3) lower end, is fixed on system shell (23) pedestal, is located at the
One halogen tungsten lamp light source (101) side；
The whole slide imaging lens group (13) is fixed on optical path shell (24) by camera connecting bracket (17), whole slide at
As being terminated with whole slide imaging detector (14) on lens group (13)；
The motorized subject table (3) is placed in the first object lens (103), the second object lens (203) and the first halogen tungsten lamp light source (101), the
Between two halogen tungsten lamp light sources (201), motorized subject table (3) edge is equipped with objective table x motor (10), objective table y motor (11)；Electricity
Dynamic object stage (3) quadrangle is equipped with magnetic (5), and glass slide pallet (4) is placed on magnetic (5), is fixed in motorized subject table
(3) on；Glass slide (6) is placed on glass slide pallet (4) and is fixed by glass slide clamp (29), and system shell is provided with load glass on (23)
Tablet tray entrance (9), for being put into glass slide pallet (4)；System shell (23) bottom is fixed with power supply (22), and power supply (22) is defeated
It is connected respectively to power input, the first halogen tungsten lamp light source (101) power input, the second halogen of LED area array light source (12) out
Tungsten light source (201) power input, acquisition and control unit (21) power input；
The acquisition and control unit (21) are fixed on system shell (23), the stage motor of acquisition and control unit (21)
Control output end is connected respectively to the control signal of objective table x motor (10), objective table y motor (11)；Acquisition and control are single
The revolving mirror control output end of first (21) is connected to the control signal of rotation mirror driver (18), rotation mirror driver (18)
Control output end is connected in the control signal of Double-face rotating reflecting mirror (8)；The Zoom control of acquisition and control unit (21)
Output end is connected to the control signal of zoom controller (19), and the control output end of zoom controller (19) is connected respectively to
The control signal of one zoom lens (104), the second zoom lens (204)；The control output end of acquisition and control unit (21)
It is connected to the control signal of filter unit controller (20), the control output end of filter unit controller (20) is connected to optical filtering
The control signal of unit (702)；The output end of whole slide imaging detector (14) is connected to acquisition and control unit (21)
Data input pin；The output end of gray scale scanning imaging detector (15) is connected to acquisition and the data input of control unit (21)
End；The output end of chromoscan camera (16) is connected to the data input pin of acquisition and control unit (21)；Acquisition and control are single
The input/output signal line of first (21) is connected on the interface (25) of system shell (23).
2. whole slide multi-pass high throughput pathological section scanner according to claim 1, which is characterized in that the optical filtering
Unit (702) is optical filter runner, prism, grating, liquid crystal tunable filter or acousto-optic turnable filter.
3. whole slide multi-pass high throughput pathological section scanner according to claim 1, which is characterized in that the two-way
Tetra- Lu Guanglu of optical path Huo Wei.
4. whole slide multi-pass high throughput pathological section scanner according to claim 1, which is characterized in that being capable of basis
Sample areas aspect ratio delimit two scanning areas automatically and the direction x or the direction y is selected to scan on glass slide (6), and raising is swept
Retouch efficiency.
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