CN106901683A - A kind of fluorescent endoscopic imgaing system - Google Patents
A kind of fluorescent endoscopic imgaing system Download PDFInfo
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- CN106901683A CN106901683A CN201710312538.XA CN201710312538A CN106901683A CN 106901683 A CN106901683 A CN 106901683A CN 201710312538 A CN201710312538 A CN 201710312538A CN 106901683 A CN106901683 A CN 106901683A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00165—Optical arrangements with light-conductive means, e.g. fibre optics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00186—Optical arrangements with imaging filters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
- A61B1/043—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances for fluorescence imaging
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
- A61B1/07—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements using light-conductive means, e.g. optical fibres
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/273—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the upper alimentary canal, e.g. oesophagoscopes, gastroscopes
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Abstract
The present invention relates to a kind of fluorescent endoscopic imgaing system multispectral imaging can not be carried out to solve the problems, such as endoscopic imaging system of the prior art.During using fluorescent endoscopic imgaing system of the invention, image-carrying fiber bundle is placed in the region to be detected of hollow tissue, the exciting light light wave according to different fluorescence probes adjusts suitable exciting light optical filter, dichroscope optical filter and launching light optical filter and enters corresponding optical path.The light source light optical filter that is excited can be made to be filtered into narrowband excitation light, the second light path reflexed to by dichroscope optical filter again afterwards and the fluorescence probe in region to be detected is excited.The fluorescence signal that fluorescence probe is launched after being excited by image acquisition and processing module after dichroscope optical filter and launching light optical filter by being gathered.So the present invention only needs to be conveniently realized by adjusting multispectral light splitting harvester the multispectral imaging of different fluorescence probes.
Description
Technical field
The present invention relates to a kind of fluorescent endoscopic imgaing system.
Background technology
Digestive system tumor is one of most common tumour in world wide.Early discovery, early treatment, improve the diagnosis water of early cancer
It is flat to have profound significance for improving survival, mitigating social economical burden.Current international research shows, endoscopy
It is the most effective approach for finding digestive system tumor.But existing endoscopic technic to there is recall rate low, rate of missed diagnosis is high etc. many asks
Topic.To solve existing issue, molecular imaging is to we provide new thinking.Molecular imaging is to intravital bioprocess
Studied in cell and molecular level, while combined with specific molecular being capable of achieving real-time, quantitative imaging using targeted probes.Will
The digestive endoscopy molecular imaging that molecular imaging technology is combined with digestive endoscopy, as realize tumor in digestive tract early diagnosis
Effective way.Current achievement in research has shown the good development and application prospect in this field.
Conventional molecular imaging equipment has following several at present：
(1) AF imaging device
Auto-fluorescence imaging (AFI) system excites blue light (ripple with xenon light through formation after blue-green rotating filtering piece
390-470nm long) and green glow (wavelength 540-560nm) direct irradiation gastrointestinal tract mucosa, except the blue light of reflection (is inhaled by barrier filter
Wavelength is received in 500-630nm) stop outer, reflection green glow and autofluorescence are caught through barrier filter by CCD, through photoelectric conversion and
Shown over the display after image reconstruction.
But AF imaging device has the disadvantages that：As long as molecular structure changes in theory, AF is just
Feature can occur to sexually revise, the optical contrast of AFI images does not embody tumour-specific optical contrast, be one and combine multiple
The result of molecular changes.Because AFI does not use selectively targeted contrast agent, thus false positive rate is higher, for inflammation with it is swollen
The differentiation of knurl acquires a certain degree of difficulty.Simultaneously because AF signal intensity is very faint, it is vulnerable to the influence of exciting light aliasing.
(2) Raman spectrum imaging
Raman spectrum imaging (RSI) is based on inelastic optical scattering phenomenon, there is provided detailed chemical information.Using Raman light
Spectrum, clinical early diagnosis is made by detecting the pernicious chemical differences and normal structure between.But of early diagnosis
Important limitation is the inefficiencies of intrinsic Raman scattering, because its dtr signal, the time for exposure is long, sensitivity is not enough and penetrates
Depth is limited, has seriously limited the technology and has been converted to clinic.
(3) confocal microscopic image
Confocal microscopic image (CLI) its principle, similar to laser confocal microscope, can make endoscopic knot of tissue
Structure amplifies 1000 times, so that clinician carries out HD in real time while endoscopic views to patient turning into
May.And Jiao's scope of copolymerization at present is only capable of providing the exciting light of 488nm, in multispectral fluorescence imaging side, mask has a clear superiority.
Fluorescein sodium needs intravenous injection as the fluorescence contrast agent of copolymerization Jiao's scope when using, though there is document to show fluorescein both at home and abroad
Sodium can be used safely, but the risk of intravenous injection compares surface sprinkling and wants high many, and security is difficult to ensure that.
(4) copolymerization Jiao scope
Copolymerization Jiao's scope is as good as with Laser Scanning Confocal Microscope in principle, using the imaging mode of " spot scan ", but this
Although kind of an imaging mode can provide high-resolution picture rich in detail, image taking speed is had a greatly reduced quality, and fluorescence is by detector
Detection, need in computer systems be converted into electrical image, later stage artificial image selection time and effort consuming.
(5) high-resolution fluorescence imaging
High-resolution fluorescence imaging (HRME), is sprayed at structural fluorescent contrast agent and is imaged by exciting.At present, often
Fluorescent contrast agent acid trypaflavine can be combined and dyeed with nucleus and intracytoplasmic DNA, RNA, by wavelength 445nm
Excite light irradiation after, the fluorescence of wavelength 515nm can be launched.But HRME can only carry out single spectrum imaging at present, nothing is gone back
Method combines various fluorescence probes carries out multispectral imaging, while lack image analysis software to carry out quantifying and qualitative point to image
Analysis.
The content of the invention
It is an object of the invention to provide a kind of fluorescent endoscopic imgaing system solving based endoscopic imaging system of the prior art
System can not carry out the problem of multispectral imaging.
To achieve the above object, a kind of fluorescent endoscopic imgaing system of the invention uses following technical scheme：
A kind of fluorescent endoscopic imgaing system, including illuminating light source module, multispectral light splitting harvester, image acquisition and processing
Module, mechano-electronic control module and for inserting in tissue to be detected to excite the image transmission optical fibre of fluorescence probe and conducting image
Beam, the multispectral light splitting harvester is arranged in camera bellows and specifically includes exciter filter switch, dichroscope filters
Switch and launching light optical-filter switcher, each switch includes can be along the runner of the pivot axis of itself, runner
Along its be provided at circumferentially spaced it is multiple away from its pivot center apart from the identical optical port installed for respective filter, also set on runner
It is equipped with for driving the runner along the actuator of the pivot axis of itself, the actuator is by mechano-electronic control module
Control；Light path between illuminating light source module, exciter filter switch and dichroscope optical filtering switch is formed for that will shine
Mingguang City source is filtered into the first light path of narrowband excitation light, and the light path between dichroscope optical filtering switch and image-carrying fiber bundle is formed
Second light path, dichroscope optical filtering switch, the light path shape between launching light optical-filter switcher and image acquisition and processing module
Into the 3rd light path for organization chart picture to be detected to be presented；Second light path is in opposite direction with the 3rd light path, the first light path with
Second light path and the 3rd light path are vertical.
Enlarging objective module is provided between dichroscope optical filtering switch and image-carrying fiber bundle in second light path,
The enlarging objective module includes enlarging objective runner and for drive amplification object lens runner around its own pivot axis
Enlarging objective runner actuator, multiple optical ports installed for enlarging objective are provided at circumferentially spaced on enlarging objective runner along it,
Distance of multiple optical ports away from its pivot center is identical.
Optical alignment coupler is provided between enlarging objective module and image-carrying fiber bundle in second light path.
Hot spot adjuster is provided with first light path between illuminating light source module and exciter filter switch.
The image-carrying fiber bundle includes many image transmission optical fibre monofilament and is wrapped in the biography of bundles of image transmission optical fibre monofilament periphery
As optical fiber outer jacket pipe.
Described image acquisition processing module includes transmitting the camera of image and for phase for receiving image-carrying fiber bundle
The image data processing module that the image that machine is received is processed, described image data processing module includes being used for original glimmering
Light image carries out denoising and is filtered with removing the Gauss of the grid image that image transmission optical fibre monofilament interval is caused in Raw fluorescence image
Ripple module, image data processing module also includes being integrated with algorithm of histogram equalization improving contrast and the life of fluoroscopic image
Into the correction module of correction fluoroscopic image.
During described image data processing module is also included for showing the correction fluoroscopic image, calculating correction fluoroscopic image
Quantitative parameter and by gained image and related data carry out classify storage server.
The camera is the CCD camera with image information conversion unit, and image information conversion unit is used to believe image
Breath is converted into data message.
Beneficial effects of the present invention are as follows：During using fluorescent endoscopic imgaing system of the invention, image-carrying fiber bundle is placed in
The region to be detected of hollow tissue, the exciting light light wave adjustment exciter filter switch according to different fluorescence probes makes properly
Exciting light optical filter be filtered into narrowband excitation light with by light source into the first light path, narrowband excitation light is by dichroscope optical filter
Dichroscope optical filter reflection in switch and go to second light path vertical with the first light path, and along the second optical line terminal
Image-carrying fiber bundle excite the fluorescence probe in region to be detected.The fluorescence signal that fluorescence probe is launched after being excited passes through the second light
Road and enter threeth light path reverse with the second light path, according to excite fluorescence adjust transmitting mating plate switch make suitable launching light
Optical filter enters tertiary circuit, the emitted smooth optical filter of fluorescence signal gathered by image acquisition and processing module after filtering again and
The fluoroscopic image in region to be detected is presented.The exciting light reflected by region to be detected but can be completely anti-by dichroscope optical filter
Penetrate without entering the 3rd light path, the final fluoroscopic image for collecting is not interfered with naturally also.So the present invention only needs to regulation
Multispectral light splitting harvester makes suitable optical filter different fluorescence probes can just be excited into corresponding light path and is gathered
Fluoroscopic image after accordingly fluorescence probe is excited, has conveniently realized the multispectral imaging of different fluorescence probes.And incident light
The form that could enter the second light path after being reflected through dichroscope optical filter is needed more to be that of avoiding other narrow spectral coverage light of interference
The final diagnosis carried out according to fluoroscopic image is adversely affected into image-carrying fiber bundle.
Brief description of the drawings
Fig. 1 is a kind of structural representation of one embodiment of fluorescent endoscopic imgaing system of the invention；
Fig. 2 is the cross-sectional view of image-carrying fiber bundle in Fig. 1；
Fig. 3 is the cross section structure schematic diagram of image-carrying fiber bundle in Fig. 1；
Fig. 4 is the flow chart that image acquisition and processing storage is carried out using the fluorescent endoscopic imgaing system in Fig. 1.
Specific embodiment
A kind of structure of fluorescent endoscopic imgaing system of the invention as shown in Figures 1 to 3, including illuminating light source module, light more
Spectrum light splitting harvester, objective lens module, image acquisition and processing module and mechano-electronic control module.
Multispectral light splitting harvester includes exciter filter switch I, dichroscope optical-filter switcher II and transmitting
Light optical-filter switcher III.Above three switch by micro-step motor control, the control signal of micro-step motor by
Mechano-electronic control module sends.
Light path between illuminating light source module, exciter filter switch and dichroscope optical filtering switch is formed for inciting somebody to action
Lighting source is filtered into the first light path of narrowband excitation light.Light path shape between dichroscope optical filtering switch and image-carrying fiber bundle
Into for exciting the fluorescence probe in tissue to be detected Raw fluorescence image to be presented or for injecting and treating for broad spectrum light source
Detect in organizing that the second light path of wide field-of-view image is presented.Dichroscope optical filtering switch, launching light optical-filter switcher and
Light path between image acquisition and processing module forms the 3rd light path for organization chart picture to be detected to be presented.Second light path and the
Three light paths it is in opposite direction, the first light path is vertical with the second light path and the 3rd light path
Wherein mechano-electronic control module 22 is that may be programmed mechanical control device, overall control exciting light optical filter controller 2,
Dichroscope optical filter wheel controller 11 and launching light optical filter controller 14 carry out rotation switching.
Illuminating light source module includes monochromatic tunable light source 1, light-conductive optic fibre 18 and hot spot adjuster 19, can be according to the need of user
The wide spectrum collimated ray for producing different-energy is adjusted, the spot size of light source incidence is adjusted by hot spot adjuster 19, shone
Mingguang City's line enters multispectral light splitting harvester by light-conductive optic fibre 18.
In order to ensure a kind of photophobism of fluorescent endoscopic imgaing system of the invention, multispectral light splitting harvester is by camera bellows
20 parcels.The motion of multispectral light splitting harvester then passes through data transfer line traffic control by mechano-electronic control module, it is to avoid
Each rotating wheel needs to open the operation of camera bellows 20, imaging system is had multispectral acquisition of information function.The case of camera bellows 20
The side opening passed through for corresponding optical fiber, power line and data wire is offered on body.
Exciter filter switch I includes that exciting light optical filter controller 2, exciting light optical filter 3, exciting light optical filter turn
Wheel 4, is provided with multiple optical ports for loading exciting light optical filter 3 on exciting light optical filter runner 4, one of optical port is not pacified
Fill exciting light optical filter and form standby eating dishes without rice or wine.Exciting light optical filter controller 2 drives exciting light optical filter runner 4 along its turn
Shaft line rotates to switch the exciting light optical filter 4 in the first light path.User can be adjusted according to the fluorescence probe for being used
Exciting light optical filter controller 2 makes suitable exciting light optical filter 4 enter the first light path, and selected exciting light optical filter 4 is filtered
The wide spectrum white light in hot spot is injected, and specific narrow-band spectrum is transmitted backward through exciting light optical filter 4 and shape
Into the narrowband excitation light that can excite fluorescence probe, while not introducing the narrow spectral coverage emergent light of other spectral coverage light interference again.In selection
During exciting light optical filter 4, Ying Yineng fully excites a length of selection gist of the light wave of used fluorescence probe.Wherein exciting light filter
Mating plate 3 is minor diameter bandpass interference filter, and its band logical spectral coverage should match with the absorption spectra of fluorescence probe used.
Dichroscope optical-filter switcher II includes dichroscope optical filter wheel controller 11, dichroscope optical filter
12nd, dichroscope runner 13.Multiple optical ports for loading dichroscope optical filter 12 are provided with dichroscope runner 13, its
In an optical port dichroscope optical filter 12 is not installed and standby eating dishes without rice or wine is formed.Dichroscope optical filter wheel controller 11 is controlled
Dichroscope runner 13 processed makes matched with the narrowband excitation light by being projected in exciter filter 3 two along its pivot axis
Enter light path to Look mirror optical filter 12, narrowband excitation light is fully reflective rear into light path next stage by dichroscope optical filter 12
Through object lens switch.Through the narrowband excitation light after object lens switch image-carrying fiber bundle is coupled to through optical alignment coupler 9
10 near-end.Wherein optical alignment coupler 9 is focus lens group, plays a part of to collimate and assemble light beam, and it will be by arrowband
Exciting light is gathered into the light beam close with the diameter of image-carrying fiber bundle 10, and impinges perpendicularly on the image-carrying fiber bundle in subordinate's light path
In 10.Image-carrying fiber bundle 10 is flexible detector, thus its distal end can smoothly stretch into inside of human body cavity tissue and be close to treat
Observation object, and narrowband excitation light is conducted into inside of human body cavity tissue, and existed with circular light facular model uniform irradiation
Detection zone.So narrowband excitation light can be excited observed object as image-carrying fiber bundle 10 is sent to distal end by its near-end
Send out and launch fluorescence signal.Observed object is stimulated and sends fluorescence signal, at the same observed object can also reflecting part it is narrow
Exciting light is formed with exciting light, visible ray is reflected and is formed reflected light.
Wherein dichroscope optical filter 12 is the logical dichroscope optical filter 12 of minor diameter long wave, its cutoff wavelength should with it is used
The absorption spectra of fluorescence probe matches.Narrowband excitation light is reflexed on objective lens module by dichroscope optical filter 12, while to be checked
The fluorescence (wavelength than exciting light is long) for surveying tissue stimulated emission is directly entered camera through dichroscope optical filter 12.And to
Back reflection or by object under test scatter exciting light by the secondary reflection of dichroscope optical filter 12, prevent it from entering into camera shape
Into spurious signal.
Object lens switch includes enlarging objective 5, object lens runner 6, object lens mounting hole 7 and object lens wheel controller 8, and object lens turn
Multiple enlarging objectives 5 are mounted with wheel 6.User can control object lens runner 6 to turn along its pivot center by object lens wheel controller 8
Move and the enlarging objective 5 of different amplification is accessed into light path.Reflected light, exciting light and the stimulated emission sent by object under test
Fluorescence signal collected by image-carrying fiber bundle 10 after, by enlarging objective 5 and form the image of amplification.Object lens wheel controller 8
By may be programmed micro-step motor control to switch the enlarging objective of different amplification.The selection of enlarging objective multiplication factor should
Diameter with image-carrying fiber bundle is adapted.Multiple optical ports for loading enlarging objective 5 are provided with object lens runner 6, its
In an optical port enlarging objective 5 is not installed and standby eating dishes without rice or wine is formed.
Wherein enlarging objective 5 is that, with the externally threaded limited remote flat-field achromatic objective lens of RMS, multiplication factor is respectively 4X,
10X, 20X, 40X, parfocalization between each object lens can be amplified to fluoroscopic image, and amplification is formed in enlarging objective conjugate point
Real image, and project in the detection chip of CCD camera 17.The multiplication factor computational methods of enlarging objective 5 are：M=Lmin/d。
M is multiplication factor, L in formulaminIt is the bond length of rectangle CCD detection chip, d is the diameter of image-carrying fiber bundle.
It is resent to after dichroscope optical-filter switcher II by the spectral signal after enlarging objective and is divided into several roads.
The exciting light and reflected light of Tissue reflectance to be detected cannot pass through dichroscope optical filter 12 but be reflected off.Fluorescence signal energy
Through dichroscope optical filter 12 and incide launching light optical-filter switcher III.Launching light optical-filter switcher III includes transmitting
Light optical filter controller 14, launching light optical filter 15, launching light optical filter runner 16, are provided with launching light optical filter runner 16
Multiple is used to load the optical port of launching light optical filter 15, and one of optical port does not install launching light optical filter 15 and forms standby sky
Mouthful.Control launching light optical filter runner 16 to rotate around it axis by launching light optical filter controller 14 to rotate, you can by difference
Launching light optical filter 15 be transferred in light path.Suitable launching light optical filter 15 is accessed into the 3rd light path, fluorescence signal is launched
Light optical-filter switcher III is gathered after filtering by image acquisition and processing module, you can show region to be detected on the display device
Raw fluorescence image.Fluorescent light beam is projected on the target surface of CCD camera 17, and CCD camera 17 is converted to the optical signal of image
Electric signal is input into server, and server is acquired and is displayed on fluorescent screen to image, it is possible to image is processed, is deposited
Store up and carry out file management.
The light shaft coaxle of the optical axis direction of launching light optical filter 15 and parallel enlarging objective 5, so by launching light optical filter
The direction of 15 fluorescence signals for projecting is in opposite direction with the narrowband excitation light for injecting optical alignment coupler 9.
17 pairs of Raw fluorescence images after launching light optical filter 15 is filtered of CCD camera are acquired.Wherein camera is
Charge-coupled image sensor (CCD) camera or CMOS complementary metal-oxide-semiconductor pipe (CMOS) camera, it is weak for receiving image intensity
Optical signal, and by sample conversion be digital picture, it is effectively imaged the faint fluorescence signal of light intensity, obtains raw fluorescence profiles
Picture.
Image acquisition and processing module includes CCD camera 17 and image real time transfer control module 21.Image real time transfer mould
Block includes gaussian filtering module, is integrated with the correction module and server of algorithm of histogram equalization.Image real time transfer is controlled
Module 21 is as follows to the method that Raw fluorescence image is processed：The first step, is calculated using the gaussian filtering in Gauss big waves module
Method carries out denoising, the grid image that image transmission optical fibre interval is caused in removal Raw fluorescence image to Raw fluorescence image；The
Two steps, the contrast of fluoroscopic image, generation correction fluoroscopic image are improved by the algorithm of histogram equalization in correction module；The
Three steps, real-time synchronization shows described correction fluoroscopic image in the server, and calculates the cell check figure in correction fluoroscopic image
Amount, long term voyage, the caryoplasm when quantitative parameter such as nuclear separation, classification storage is carried out by gained image and related data.
Wherein launching light optical filter 15 is minor diameter bandpass interference filter, the hair of its band logical spectral coverage covering fluorescer used
Spectral peak is penetrated, and it is not overlap with the band logical spectral coverage of exciting light optical filter used.Thus the emitted smooth optical filter 15 of fluorescence signal it
Afterwards, non-fluorescence signal can be filtered out.The spectrum picture that non-fluorescence signal is Chong Die with fluorescence signal and influence is finally gathered is avoided,
The inaccurate erroneous judgement that may cause inspection result of spectrum picture collection.
Image-carrying fiber bundle 10 is used to be transmitted to exciting light the detection zone of inside of human body hollow organ, and is collected from detection zone
The optical signallings such as exciting light, fluorescence interested and be transmitted to external imaging.Image-carrying fiber bundle 10 is specifically comprising a branch of flexible, small
External diameter, high-resolution, optical transport Penetration ration image transmission optical fibre monofilament 24 high and it is enclosed within the bundles of periphery of image transmission optical fibre monofilament 24
Image transmission optical fibre outer tube 23.The design of image-carrying fiber bundle 10 smoothly can reach the skies such as esophagus, stomach through the opening such as human mouth
Heart organ, outgoing exciting light is conducted into inside of human body cavity tissue, and with circular light facular model uniform irradiation in detection
Region.Optical fiber outer jacket pipe 23 is made using medical grade polypropylene acid amides or polytetrafluoroethylmaterial material.
Described Optic transmission fiber 18 is silica fibre, is about 100cm, diameter 2.5mm, is equipped with SMA905 output ports.
The wide spectrum light that broad spectrum light source 1 is produced is conducted by Optic transmission fiber 18, and the hot spot regulation for passing through fiber distal end
Device 19 forms the collimated ray of special diameter.
Wherein broad spectrum light source 1 can be high power xenon lamp, halogen lamp light source or mercury lamp light source, and its outgoing spectrum segment covering can
See light near infrared wavelength region.
The method of present invention display tissue picture to be detected is as follows：Observed object is produced after narrowband excitation light irradiation glimmering
Optical signal, fluorescence signal is amplified after being received by image-carrying fiber bundle 10 by being exaggerated object lens 5 after image-carrying fiber bundle, glimmering after amplification
Light light beam is projected on the target surface of CCD camera by dichroscope optical filter runner 13 and launching light optical filter 15.CCD camera
The optical signal of image is converted into the server in electric signal input picture acquisition processing module, server is acquired to image
And show on a display screen, it is possible to processed image, stored and carried out file management.
Flow chart such as Fig. 4 institutes of image acquisition and processing storage are carried out using a kind of fluorescent endoscopic imgaing system of the invention
Show, comprise the following steps：
1st, opening device power supply, including CCD camera 17 power supply, the power supply and computer power of broad spectrum light source 1；
2nd, in the distal end of image-carrying fiber bundle 10 insertion tissue cavity and it will be made to be close to object under test, now can be by
CCD camera collects Raw fluorescence image and wide field-of-view image；
3rd, the image of optical signalling is converted into data signal and is input into computer by CCD camera 17；
4th, denoising is carried out using Gaussian filter algorithm to Raw fluorescence image, in removal Raw fluorescence image between optical fiber
Every the grid image for causing, then by the contrast of algorithm of histogram equalization raising fluoroscopic image, generation corrects fluoroscopic image,
And real-time synchronization shows described correction fluoroscopic image over the display, and the cell check figure in fluorescence associated image can be calculated
Amount, long term voyage, the caryoplasm when quantitative parameter such as nuclear separation；
The quantitative parameters such as the 4th, computer high-ranking officers positive image and nucleus amount, long term voyage, nucleocytoplasmic ratio, nuclear separation are displayed in aobvious
Show on device；
5th, repeat step 2-4, obtains a number of fluoroscopic image and related quantitative parameter；
6th, classification folder is set up, the image of collection and related quantitative parameter are stored in file；
7；Cut-out device power supply (DPS) closes computer.
In other embodiments, image card input computer can also be converted into data signal with by optical signalling.
The VEGF antibody two that EGFR antibody and Alexa Fluor680 are marked is marked with by FITC with nude mice model of colon cancer
Kind fluorescence probe is carried out as a example by multispectral imaging, and the side of multispectral imaging is carried out using a kind of fluorescent endoscopic imgaing system of the invention
Method is as follows：
According to two kinds of spectroscopic properties of fluorescence probe of FITC, Alexa Fluor680 for being used, suitable two are selected
Set exciting light optical filter 488nm and 645nm, two sets of dichroscope optical filters 490nm and 690nm, two sets of launching light optical filters
The combination of 525nm and 710nm.
Broad spectrum light source 1, camera 17 and computer are opened, the distal end of image-carrying fiber bundle is aligned and is close to detected part.
First FITC mark EGFR antibody is carried out being excited into picture, exciting light optical filter runner 4 is switched respectively, dichroscope turns
Wheel 13 and launching light optical filter runner 16 make one group of exciting light optical filter 3, dichroscope optical filter 12 and launching light optical filter 15
Into light path, excite light irradiation to detect thing with narrow spectral coverage, CCD camera 17 is shot first group of Raw fluorescence image.
Carry out being excited into picture in the VEGF antibody for marking Alexa Fluor680, exciting light optical filter runner is switched respectively
4, dichroscope runner 13 and launching light optical filter runner 16 make another group of exciting light optical filter 3, dichroscope optical filter 12 and
Launching light optical filter 15 enters light path, excites light irradiation to detect thing with narrow spectral coverage, CCD camera 17 is shot second group of Raw fluorescence
Image.
Intrinsic interval when being imaged due to image-carrying fiber bundle 10 between image transmission optical fibre monofilament 24 therein can be produced in imaging
Raw lattice, has a strong impact on picture quality and the interpretation to image information, even more cannot carry out picture number to gained image
According to treatment and quantitative analysis.So needing to use image real time transfer control module 21 to process Raw fluorescence image.
Image real time transfer control module 21 reads two groups of Raw fluorescence images, and two groups of Raw fluorescence images are used respectively
Gaussian filter algorithm carries out denoising, and optical fiber is spaced the grid image for causing in removal Raw fluorescence image, then by Nogata
Figure equalization algorithm improves the contrast of fluoroscopic image, generation correction fluoroscopic image, and real-time synchronization display institute over the display
The correction fluoroscopic image stated.
In the present embodiment, described Gaussian filter algorithm and algorithm of histogram equalization specifically includes following steps：
Two groups of Raw fluorescence images are read respectively, with (- 1)x+yBeing multiplied by input picture carries out coordinate transform, using discrete Fu
In leaf transformation calculate image DFT, be multiplied by F (μ, ν) with filter function H (μ, ν) and calculate anti-DFT, obtain result multiplied by with (-
1)x+yObtain the image after gaussian filtering.
The two dimensional form of gaussian filtering is as follows：
D (μ, ν) is blur radius r, wherein r in formula2=μ2+ν2；σ is the standard deviation of normal distribution.
When carrying out image procossing by algorithm of histogram equalization, specific mapping method is as follows：
Wherein n is the summation of pixel in image, nkIt is the number of pixels of current gray level level, L is possible gray scale in image
Level sum.
It is that can obtain image intensity value to map and calculate the cell in fluorescence associated image by image intensity value by above formula
Nuclear volume, long term voyage, the caryoplasm when quantitative parameter such as nuclear separation, and gained image and related data can be carried out classification storage.
The fluorescence endoscope imaging system of the present embodiment can be switched in light path different optical filters, to meet different spies
For the requirement of filtering apparatus imaging, so as to realize to various fluorescence probe fast imagings.Further, since each using Electronic control
The motor of runner, need not open camera bellows during switching, can be switched with quick electric, it is to avoid the interference of external stray light is reduced simultaneously
The loss of optical signalling in optical system.And due to having carried out image procossing, the quality of fluoroscopic image is improve, and can be right
Image carries out quantitative analysis.
Claims (8)
1. a kind of fluorescent endoscopic imgaing system, it is characterised in that：Including illuminating light source module, multispectral light splitting harvester, figure
As acquisition processing module, mechano-electronic control module and for inserting in tissue to be detected to excite fluorescence probe and conducting image
Image-carrying fiber bundle, the multispectral light splitting harvester is arranged in camera bellows and specifically includes exciter filter switch, two
To Look mirror optical filtering switch and launching light optical-filter switcher, each switch includes can turning along the pivot axis of itself
Wheel, on runner along its be provided at circumferentially spaced it is multiple away from its pivot center apart from the identical optical port installed for respective filter,
It is additionally provided with runner for driving the runner along the actuator of the pivot axis of itself, the actuator is by mechanical electric
Sub- control module control；Light path shape between illuminating light source module, exciter filter switch and dichroscope optical filtering switch
Into the first light path for lighting source to be filtered into narrowband excitation light, between dichroscope optical filtering switch and image-carrying fiber bundle
Light path formed the second light path, dichroscope optical filtering switch, launching light optical-filter switcher and image acquisition and processing module it
Between light path form the 3rd light path for organization chart picture to be detected to be presented；Second light path is in opposite direction with the 3rd light path,
First light path is vertical with the second light path and the 3rd light path.
2. a kind of fluorescent endoscopic imgaing system according to claim 1, it is characterised in that：In second light path in two to
Enlarging objective module is provided between Look mirror optical filtering switch and image-carrying fiber bundle, the enlarging objective module includes enlarging objective
Runner and for drive amplification object lens runner around its own pivot axis enlarging objective runner actuator, enlarging objective turn
On wheel multiple optical ports installed for enlarging objective, distance phase of multiple optical ports away from its pivot center are provided at circumferentially spaced along it
Together.
3. a kind of fluorescent endoscopic imgaing system according to claim 2, it is characterised in that：In amplification in second light path
Optical alignment coupler is provided between objective lens module and image-carrying fiber bundle.
4. a kind of fluorescent endoscopic imgaing system according to claim 1, it is characterised in that：In illumination in first light path
Hot spot adjuster is provided between light source module and exciter filter switch.
5. a kind of fluorescent endoscopic imgaing system according to any one of Claims 1 to 4, it is characterised in that：The biography picture
Fibre bundle includes many image transmission optical fibre monofilament and is wrapped in the image transmission optical fibre outer tube of bundles of image transmission optical fibre monofilament periphery.
6. a kind of fluorescent endoscopic imgaing system according to claim 5, it is characterised in that：Described image acquisition processing module
Including transmitting the image that the camera of image and the image for being received to camera are processed for receiving image-carrying fiber bundle
Data processing module, described image data processing module includes original to remove for carrying out denoising to Raw fluorescence image
The gaussian filtering module of the grid image that image transmission optical fibre monofilament interval is caused in fluoroscopic image, image data processing module also includes
Algorithm of histogram equalization is integrated with to improve the contrast of fluoroscopic image and generate the correction module of correction fluoroscopic image.
7. a kind of fluorescent endoscopic imgaing system according to claim 6, it is characterised in that：Described image data processing module
Also include for showing the correction fluoroscopic image, calculating quantitative parameter in correction fluoroscopic image and by gained image and correlation
Data carry out the server of classification storage.
8. a kind of fluorescent endoscopic imgaing system according to claim 6, it is characterised in that：The camera is with image letter
The CCD camera of conversion unit is ceased, image information conversion unit is used to for image information to be converted into data message.
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