CN207613757U - Fluorescent endoscopic imgaing system - Google Patents
Fluorescent endoscopic imgaing system Download PDFInfo
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- CN207613757U CN207613757U CN201720494696.7U CN201720494696U CN207613757U CN 207613757 U CN207613757 U CN 207613757U CN 201720494696 U CN201720494696 U CN 201720494696U CN 207613757 U CN207613757 U CN 207613757U
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Abstract
The utility model is related to a kind of fluorescent endoscopic imgaing systems to solve the problems, such as that endoscopic imaging system in the prior art cannot carry out multispectral imaging.Using the utility model fluorescent endoscopic imgaing system when, image-carrying fiber bundle is placed in the area to be tested of hollow tissue, adjusting suitable exciting light optical filter, dichroscope optical filter and transmitting light optical filter according to the exciting light light wave of different fluorescence probes enters corresponding optical path.The light source light optical filter that is excited can be made to be filtered into narrowband excitation light, the second light path is reflexed to by dichroscope optical filter again later and excites the fluorescence probe of area to be tested.The fluorescence signal that fluorescence probe emits after being excited is acquired after dichroscope optical filter and transmitting light optical filter by image acquisition and processing module.So the utility model only needs to adjust multispectral light splitting harvester and can conveniently realize the multispectral imaging of different fluorescence probes.
Description
Technical field
The utility model is related to a kind of fluorescent endoscopic imgaing systems.
Background technology
Digestive system tumor is one of most common tumour in world wide.Early discovery, early treatment improve the diagnosis water of early cancer
It is flat to have profound significance for improving survival, mitigating social economical burden.International research shows endoscopy at present
It is the most effective approach for finding digestive system tumor.However that there are recall rates is low for existing endoscopic technic, rate of missed diagnosis height etc. is many to ask
Topic.To solve existing issue, molecular imaging is to we provide new thinking.Molecular imaging is to intravital bioprocess
It is studied in cell and molecular level, while being combined with specific molecular using targeted probes and real-time, quantitative imaging can be achieved.It will
The digestive endoscopy molecular imaging that molecular imaging technology is combined with digestive endoscopy becomes and realizes tumor in digestive tract early diagnosis
Effective way.Current achievement in research has shown the good development and application foreground in this field.
Currently used molecular imaging equipment has following several：
(1) autofluorescence imaging device
Auto-fluorescence imaging (AFI) system is with xenon light through formation excitation blue light (wave after blue-green rotating filtering piece
Long 390-470nm) and green light (wavelength 540-560nm) direct irradiation gastrointestinal tract mucosa, except the blue light of reflection (is inhaled by barrier filter
Wavelength is received in 500-630nm) blocking is outer, reflect green light and autofluorescence captured through barrier filter by CCD, through photoelectric conversion and
It is shown over the display after image reconstruction.
But autofluorescence imaging device has the disadvantages that：As long as theoretically molecular structure changes, autofluorescence
Feature can occur to sexually revise, the optical contrast of AFI images does not embody tumour-specific optical contrast, be one combine it is multiple
The result of molecular changes.Since AFI does not use selectively targeted contrast agent, thus false positive rate is higher, for inflammation and swells
The differentiation of tumor acquires a certain degree of difficulty.Simultaneously because autofluorescence signal strength is very faint, it is vulnerable to the influence of exciting light aliasing.
(2) Raman spectrum imaging
Raman spectrum imaging (RSI) is to be based on inelastic optical scattering phenomenon, provides detailed chemical information.Utilize Raman light
Spectrum makes clinical early diagnosis by detecting pernicious chemical differences between normal structure.However one of early diagnosis
Important limitation is the inefficiencies of intrinsic Raman scattering, because its dtr signal, the time for exposure is long, sensitivity is insufficient and penetrates
Depth is limited, has seriously limited the technology and has been converted to clinic.
(3) confocal microscopic image
Confocal microscopic image (CLI) its principle is similar to laser confocal microscope, can make endoscopic knot of tissue
Structure amplifies 1000 times, becomes so that clinician carries out patient while endoscopic views histopathological diagnosis in real time
It may.And be copolymerized burnt scope at present and be only capable of providing the exciting light of 488nm, in multispectral fluorescence imaging side, mask has a clear superiority.
Fluorescence contrast agent of the fluorescein sodium as the burnt scope of copolymerization, when use, need to be injected intravenously, though there is document to show fluorescein both at home and abroad
Sodium can be used safely, but the risk being injected intravenously compares surface sprinkling and wants high more, and safety is difficult to ensure.
(4) it is copolymerized burnt scope
The burnt scope of copolymerization is no different with Laser Scanning Confocal Microscope in principle, is all made of the imaging mode of " spot scan ", however this
Although kind of an imaging mode can provide high-resolution clear image, image taking speed is had a greatly reduced quality, and fluorescence is by detector
Detection, need to be converted into electrical image in computer systems, and later stage artificial image selects time and effort consuming.
(5) high-resolution fluorescence imaging
High-resolution fluorescence imaging (HRME) is imaged by excitation is sprayed at structural fluorescent contrast agent.Currently, often
Fluorescent contrast agent acid trypaflavine can with nucleus and intracytoplasmic DNA, RNA in conjunction with and dye, by wavelength 445nm
Exciting light irradiation after, the fluorescence of wavelength 515nm can be launched.However HRME can only carry out single spectrum imaging at present, go back nothing
Method combines a variety of fluorescence probes and carries out multispectral imaging, while lacking image analysis software and carrying out quantitative and qualitative point to image
Analysis.
Utility model content
The purpose of this utility model is to provide fluorescent endoscopic imgaing systems to solve based endoscopic imaging system in the prior art
The problem of system cannot carry out multispectral imaging.
To achieve the above object, the fluorescent endoscopic imgaing system of the utility model uses following technical scheme：
Fluorescent endoscopic imgaing system, including illuminating light source module, multispectral light splitting harvester, image acquisition and processing mould
Block, mechano-electronic control module and for being placed in tissue to be detected to excite the image transmission optical fibre of fluorescence probe and conducting image
Beam, the multispectral light splitting harvester is set in camera bellows and specifically includes exciter filter switch, dichroscope filters
Switch and transmitting light optical-filter switcher, each switch includes can be on the runner, runner of the pivot axis of itself
It is provided at circumferentially spaced and multiple also sets in the identical optical port installed for respective filter, runner away from its pivot center along it
It is equipped with for driving the runner along the actuator of the pivot axis of itself, the actuator is by mechano-electronic control module
Control；Light path between illuminating light source module, exciter filter switch and dichroscope optical filtering switch is formed for that will shine
Mingguang City source is filtered into the first light path of narrowband excitation light, and the light path between dichroscope optical filtering switch and image-carrying fiber bundle is formed
Second light path, the light path shape between dichroscope optical filtering switch, transmitting light optical-filter switcher and image acquisition and processing module
At the third light path of organization chart picture to be detected for rendering；The direction of second light path and third light path on the contrary, the first light path with
Second light path and third light path are vertical.
Enlarging objective module is provided between dichroscope optical filtering switch and image-carrying fiber bundle in second light path,
The enlarging objective module include enlarging objective runner and for drive amplification object lens runner around its own pivot axis
Enlarging objective runner actuator, it is provided at circumferentially spaced multiple optical ports installed for enlarging objective to enlarging objective runner upper edge,
Distance of multiple optical ports away from its pivot center is identical.
Optical alignment coupler is provided between enlarging objective module and image-carrying fiber bundle in second light path.
Hot spot adjuster is provided in first light path between illuminating light source module and exciter filter switch.
The image-carrying fiber bundle includes more image transmission optical fibre monofilament and the biography for being wrapped in bundles of image transmission optical fibre monofilament periphery
As optical fiber outer jacket pipe.
Described image acquisition processing module includes camera for receiving the transmitted image of image-carrying fiber bundle and for phase
The image data processing module that the image that machine is received is handled, described image data processing module include for original glimmering
Light image is carried out denoising and is filtered with the Gauss for removing grid image caused by image transmission optical fibre monofilament interval in Raw fluorescence image
Wave module, image data processing module further include being integrated with algorithm of histogram equalization with the contrast for improving fluorescent image and life
At the correction module of correction fluorescent image.
Described image data processing module further includes for showing the correction fluorescent image, calculating in correction fluorescent image
Quantitative parameter and gained image and related data are carried out to the server of classification storage.
The camera is the CCD camera with image information conversion unit, and image information conversion unit is for believing image
Breath is converted into data information.
The beneficial effects of the utility model are as follows：Using the utility model fluorescent endoscopic imgaing system when, will pass as light
Fine beam is placed in the area to be tested of hollow tissue, and exciter filter switching is adjusted according to the exciting light light wave of different fluorescence probes
Device makes suitable exciting light optical filter enter the first light path so that light source is filtered into narrowband excitation light, and narrowband excitation light is by dichroic
Dichroscope optical filter in mirror optical-filter switcher is reflected and is gone in the second light path vertical with the first light path, and along second
The fluorescence probe of the image-carrying fiber bundle excitation area to be tested of optical line terminal.The fluorescence signal that fluorescence probe emits after being excited is saturating
It crosses the second light path and enters the third light path reversed with the second light path, made properly according to excitation fluorescence adjustment transmitting mating plate switch
Transmitting light optical filter enter tertiary circuit, the emitted smooth optical filter of fluorescence signal filter again after by image acquisition and processing module
Acquire and present the fluorescent image of area to be tested.It can but be filtered by dichroscope by the exciting light that area to be tested is reflected
Piece is fully reflective without entering third light path, does not interfere with final collected fluorescent image naturally also.So this practicality is new
Type only need to adjust multispectral light splitting harvester make suitable optical filter enter corresponding light path can excite it is different glimmering
Light probe simultaneously acquires the fluorescent image after corresponding fluorescence probe is excited, conveniently realized different fluorescence probes it is multispectral at
Picture.And incident light needs the form that could enter the second light path after dichroscope optical filter reflects more to be that of avoiding other do
Narrow spectral coverage light is disturbed to enter image-carrying fiber bundle and adversely affect the final diagnosis carried out according to fluorescent image.
Description of the drawings
Fig. 1 is the structural schematic diagram of one embodiment of the fluorescent endoscopic imgaing system of the utility model；
Fig. 2 is the cross-sectional view of image-carrying fiber bundle in Fig. 1；
Fig. 3 is the cross section structure schematic diagram of image-carrying fiber bundle in Fig. 1；
Fig. 4 is the flow chart that image acquisition and processing storage is carried out using the fluorescent endoscopic imgaing system in Fig. 1.
Specific implementation mode
The structure of the fluorescent endoscopic imgaing system of the utility model is as shown in Figures 1 to 3, including illuminating light source module, mostly light
Spectrum light splitting harvester, objective lens module, image acquisition and processing module and mechano-electronic control module.
Multispectral light splitting harvester includes exciter filter switch I, dichroscope optical-filter switcher II and transmitting
Light optical-filter switcher III.Above three switch is controlled by micro-step motor, the control signal of micro-step motor by
Mechano-electronic control module is sent out.
Light path formation between illuminating light source module, exciter filter switch and dichroscope optical filtering switch is used for will
Lighting source is filtered into the first light path of narrowband excitation light.Light path shape between dichroscope optical filtering switch and image-carrying fiber bundle
It is waited at for exciting the fluorescence probe in tissue to be detected so that Raw fluorescence image is presented or for being injected for broad spectrum light source
It detects in tissue so that the second light path of wide field-of-view image is presented.Dichroscope optical filtering switch, transmitting light optical-filter switcher and
Light path between image acquisition and processing module forms the third light path of organization chart picture to be detected for rendering.Second light path and the
The direction of three light paths is on the contrary, the first light path and the second light path and third light path are vertical
Wherein mechano-electronic control module 22 is that may be programmed mechanical control device, overall control exciting light optical filter controller 2,
Dichroscope optical filter wheel controller 11 and transmitting light optical filter controller 14 carry out rotation switching.
Illuminating light source module includes monochromatic tunable light source 1, light-conductive optic fibre 18 and hot spot adjuster 19, can be according to the need of user
The wide spectrum collimated ray for generating different-energy is adjusted, the spot size of light source incidence is adjusted by hot spot adjuster 19, is shone
Mingguang City's line enters multispectral light splitting harvester by light-conductive optic fibre 18.
In order to ensure the utility model fluorescent endoscopic imgaing system photophobism, multispectral light splitting harvester is by camera bellows
20 packages.The movement of multispectral light splitting harvester avoids then by mechano-electronic control module by data transmission line traffic control
Each rotating wheel needs to open the operation of camera bellows 20, and imaging system is made to have the function of multispectral acquisition of information.The case of camera bellows 20
The side opening passed through for corresponding optical fiber, power cord and data line is offered on body.
Exciter filter switch I includes exciting light optical filter controller 2, exciting light optical filter 3, exciting light optical filter turn
4 are taken turns, multiple optical ports for loading exciting light optical filter 3 are provided on exciting light optical filter runner 4, one of optical port is not pacified
It fills exciting light optical filter and forms spare eat dishes without rice or wine.Exciting light optical filter controller 2 drives exciting light optical filter runner 4 along its turn
Shaft line rotation is to switch the exciting light optical filter 4 being located in the first light path.User can be adjusted according to used fluorescence probe
Exciting light optical filter controller 2 makes suitable exciting light optical filter 4 enter the first light path, the filtering of selected exciting light optical filter 4
The wide spectrum white light in hot spot is injected, and specific narrow-band spectrum is enable to transmit simultaneously shape backward through exciting light optical filter 4
At the narrowband excitation light that can excite fluorescence probe, while the narrow spectral coverage emergent light of other spectral coverage light interference is not introduced again.It is selecting
When exciting light optical filter 4, Ying Yineng fully excites a length of selection gist of light wave of used fluorescence probe.Wherein exciting light is filtered
Mating plate 3 is minor diameter bandpass interference filter, and band logical spectral coverage should match with the absorption spectra of fluorescence probe used.
Dichroscope optical-filter switcher II includes dichroscope optical filter wheel controller 11, dichroscope optical filter
12, dichroscope runner 13.Multiple optical ports for loading dichroscope optical filter 12 are provided on dichroscope runner 13,
In an optical port dichroscope optical filter 12 is not installed and forms spare eat dishes without rice or wine.Dichroscope optical filter wheel controller 11 is controlled
Dichroscope runner 13 processed makes and the narrowband excitation light matched two by being projected in exciter filter 3 along its pivot axis
Enter light path to Look mirror optical filter 12, narrowband excitation light by dichroscope optical filter 12 it is fully reflective after into light path next stage and
Across object lens switch.Narrowband excitation light after object lens switch is coupled to image-carrying fiber bundle through optical alignment coupler 9
10 proximal end.Wherein optical alignment coupler 9 is focus lens group, plays the role of collimation and aggregation light beam, will be by narrowband
Exciting light is gathered into the light beam close with the diameter of image-carrying fiber bundle 10, and impinges perpendicularly on the image-carrying fiber bundle in subordinate's light path
In 10.Image-carrying fiber bundle 10 is flexible detector, thus its distal end can smoothly be stretched into inside of human body cavity tissue and be close to wait for
Object is observed, and narrowband excitation light is conducted into inside of human body cavity tissue, and is existed with circular light facular model uniform irradiation
Detection zone.So narrowband excitation light can be such that observed object is excited as image-carrying fiber bundle 10 is sent to distal end by its proximal end
It sends out and emits fluorescence signal.Observed object is stimulated and sends out fluorescence signal, at the same observed object can also reflective portion it is narrow
Exciting light is formed with exciting light, is reflected visible light and is formed reflected light.
Wherein dichroscope optical filter 12 is that minor diameter long wave leads to dichroscope optical filter 12, cutoff wavelength should with it is used
The absorption spectra of fluorescence probe matches.Narrowband excitation light is reflected by dichroscope optical filter 12 on objective lens module, while to be checked
The fluorescence of survey tissue stimulated emission is (longer than the wavelength of exciting light) to be directly entered camera through dichroscope optical filter 12.And to
Back reflection or by object under test scattering exciting light by 12 secondary reflection of dichroscope optical filter, prevent it from entering camera shape
At spurious signal.
Object lens switch includes enlarging objective 5, object lens runner 6, object lens mounting hole 7 and object lens wheel controller 8, and object lens turn
It is mounted with multiple enlarging objectives 5 on wheel 6.User can control object lens runner 6 by object lens wheel controller 8 and turn along its pivot center
It moves and the enlarging objective of different amplification 5 is accessed into light path.Reflected light, exciting light and the stimulated emission sent out by object under test
Fluorescence signal collected by image-carrying fiber bundle 10 after, by enlarging objective 5 and form the image of amplification.Object lens wheel controller 8
By programmable micro-step motor control to switch the enlarging objective of different amplification.The selection of enlarging objective amplification factor is answered
It is adapted with the diameter of image-carrying fiber bundle.Multiple optical ports for loading enlarging objective 5 are provided on object lens runner 6,
In an optical port enlarging objective 5 is not installed and forms spare eat dishes without rice or wine.
Wherein enlarging objective 5 is with the externally threaded limited remote flat-field achromatic objective lens of RMS, and amplification factor is respectively 4X,
10X, 20X, 40X, parfocalization between each object lens, can be amplified fluorescent image, and amplification is formed in enlarging objective conjugate point
Real image, and project in the detection chip of CCD camera 17.5 amplification factor computational methods of enlarging objective are：M=Lmin/d。
M is amplification factor, L in formulaminFor the bond length of rectangle CCD detection chip, d is the diameter of image-carrying fiber bundle.
Spectral signal after enlarging objective is divided into several roads after being resent to dichroscope optical-filter switcher II.
The exciting light and reflected light of Tissue reflectance to be detected can not penetrate dichroscope optical filter 12 but be reflected off.Fluorescence signal energy
Through dichroscope optical filter 12 and it is incident on transmitting light optical-filter switcher III.It includes transmitting to emit light optical-filter switcher III
Light optical filter controller 14, transmitting light optical filter 15, transmitting light optical filter runner 16, emit and are provided on light optical filter runner 16
Multiple optical ports for loading transmitting light optical filter 15, one of optical port do not install transmitting light optical filter 15 and form spare sky
Mouthful.Axis rotation is rotated around it by emitting the control transmitting light optical filter of light optical filter controller 14 runner 16, you can will be different
Transmitting light optical filter 15 be transferred in light path.Light optical filter 15 will suitably be emitted and access third light path, fluorescence signal is launched
Light optical-filter switcher III is acquired after filtering by image acquisition and processing module, you can shows area to be tested on the display device
Raw fluorescence image.Fluorescent light beam projects on the target surface of CCD camera 17, and CCD camera 17 is converted to the optical signal of image
Electric signal inputs server, and server is acquired image and is shown on fluorescent screen, and can be handled image, be deposited
Storage and progress file management.
The light shaft coaxle for emitting the optical axis direction and parallel enlarging objective 5 of light optical filter 15, so by transmitting light optical filter
The direction of 15 fluorescence signals projected is opposite with the direction of narrowband excitation light of optical alignment coupler 9 is injected.
CCD camera 17 by emitting 15 filtered Raw fluorescence image of light optical filter to being acquired.Wherein camera is
Charge coupling device (CCD) camera or CMOS complementary metal-oxide-semiconductor pipe (CMOS) camera, it is weak for receiving image intensity
Optical signal, and be digital picture by sample conversion, be effectively imaged the faint fluorescence signal of light intensity, obtain raw fluorescence profiles
Picture.
Image acquisition and processing module includes CCD camera 17 and image real time transfer control module 21.Image real time transfer mould
Block includes gaussian filtering module, is integrated with the correction module and server of algorithm of histogram equalization.Image real time transfer controls
The method that module 21 handles Raw fluorescence image is as follows：The first step is calculated using Gauss big waves mould gaussian filtering in the block
Method carries out denoising to Raw fluorescence image, removes grid image caused by image transmission optical fibre interval in Raw fluorescence image；The
Two steps improve the contrast of fluorescent image by straightening die algorithm of histogram equalization in the block, generate correction fluorescent image；The
Three steps, real-time synchronization shows the correction fluorescent image in the server, and calculates the cell check figure in correction fluorescent image
Gained image and related data are carried out classification storage by amount, long term voyage, caryoplasm when quantitative parameters such as nuclear separation.
Wherein transmitting light optical filter 15 is minor diameter bandpass interference filter, the hair of band logical spectral coverage covering fluorescer used
Spectral peak is penetrated, and not be overlapped with the band logical spectral coverage of exciting light optical filter used.Thus the emitted smooth optical filter of fluorescence signal 15 it
Afterwards, non-fluorescence signal can be filtered out.The spectrum picture that non-fluorescence signal is Chong Die with fluorescence signal and influence finally acquires is avoided,
Spectrum picture acquisition inaccuracy may cause the erroneous judgement of inspection result.
Image-carrying fiber bundle 10 is used to exciting light being transmitted to the detection zone of inside of human body hollow organ, and is collected from detection zone
The optical signallings such as interested exciting light, fluorescence and be transmitted to external imaging.Image-carrying fiber bundle 10 includes specifically a branch of flexible, small
The high image transmission optical fibre monofilament 24 of outer diameter, high-resolution, optical transport Penetration ration and it is sleeved on the periphery of bundles of image transmission optical fibre monofilament 24
Image transmission optical fibre outer tube 23.The design of image-carrying fiber bundle 10 smoothly can reach the skies such as esophagus, stomach through openings such as human mouths
Outgoing exciting light is conducted into inside of human body cavity tissue, and is being detected with circular light facular model uniform irradiation by heart organ
Region.Optical fiber outer jacket pipe 23 is made of medical grade polypropylene amide or polytetrafluoroethylene material.
The Optic transmission fiber 18 is silica fibre, is about 100cm, diameter 2.5mm, is equipped with SMA905 output ports.
The wide spectrum light that broad spectrum light source 1 generates is conducted by Optic transmission fiber 18, and is adjusted by the hot spot of fiber distal end
Device 19 forms the collimated ray of special diameter.
Wherein broad spectrum light source 1 can be high power xenon lamp, halogen lamp light source or mercury lamp light source, and outgoing spectrum segment covering can
It is light-exposed to arrive near infrared wavelength region.
The utility model shows that the method for tissue picture to be detected is as follows：Observed object produces after being irradiated by narrowband excitation light
Raw fluorescence signal, fluorescence signal is amplified after being received by image-carrying fiber bundle 10 by being amplified object lens 5 after image-carrying fiber bundle, after amplification
Fluorescent light beam by dichroscope optical filter runner 13 and transmitting light optical filter 15, project on the target surface of CCD camera.CCD
The optical signal of image is converted to the server in electric signal input picture acquisition processing module by camera, and server carries out image
It acquires and shows on a display screen, and can be handled image, stored and be carried out file management.
Flow chart such as Fig. 4 institutes of image acquisition and processing storage are carried out using the fluorescent endoscopic imgaing system of the utility model
Show, includes the following steps：
1, opening device power supply includes the power supply and computer power of the power supply of CCD camera 17, broad spectrum light source 1；
2, the distal end of image-carrying fiber bundle 10 is inserted into tissue cavity and it is made to be close to object under test, it at this time can be by
CCD camera collects Raw fluorescence image and wide field-of-view image；
3, CCD camera 17 converts the image of optical signalling to digital signal and inputs computer；
4, denoising is carried out using Gaussian filter algorithm to Raw fluorescence image, removed in Raw fluorescence image between optical fiber
The grid image caused by, then by the contrast of algorithm of histogram equalization raising fluorescent image, correction fluorescent image is generated,
And real-time synchronization shows the correction fluorescent image over the display, and the cell check figure in fluorescence associated image can be calculated
Amount, long term voyage, caryoplasm when quantitative parameters such as nuclear separation；
4, computer high-ranking officers positive image and nucleus amount, long term voyage, nucleocytoplasmic ratio, the quantitative parameters such as nuclear separation are shown in aobvious
Show on device；
5, step 2-4 is repeated, a certain number of fluorescent images and related quantitative parameter are obtained；
6, classification folder is established, the image of acquisition and related quantitative parameter are stored in file；
7；It cuts off equipment power supply and closes computer.
In other embodiments, image card can also be inputted to computer to convert optical signalling to digital signal.
With the colon cancer in nude mice model VEGF antibody two for being marked EGFR antibody and Alexa Fluor680 labels by FITC
For kind fluorescence probe carries out multispectral imaging, the fluorescent endoscopic imgaing system of the utility model is used to carry out the side of multispectral imaging
Method is as follows：
According to used FITC, the spectroscopic properties of two kinds of fluorescence probes of Alexa Fluor680, suitable two are selected
Exciting light optical filter 488nm and 645nm are covered, two sets of dichroscope optical filters 490nm and 690nm, two sets emit light optical filters
The combination of 525nm and 710nm.
Broad spectrum light source 1, camera 17 and computer are opened, the distal end of image-carrying fiber bundle is aligned and is close to detected part.
Excitation imaging first is carried out to FITC label EGFR antibody, switches exciting light optical filter runner 4 respectively, dichroscope turns
Wheel 13 and transmitting light optical filter runner 16 make one group of exciting light optical filter 3, dichroscope optical filter 12 and transmitting light optical filter 15
Into light path, detection object is irradiated with narrow spectral coverage exciting light, CCD camera 17 is made to shoot first group of Raw fluorescence image.
Excitation imaging is carried out in the VEGF antibody marked to Alexa Fluor680, switches exciting light optical filter runner respectively
4, dichroscope runner 13 and transmitting light optical filter runner 16 make another group of exciting light optical filter 3, dichroscope optical filter 12 and
Emit light optical filter 15 and enter light path, irradiates detection object with narrow spectral coverage exciting light, CCD camera 17 is made to shoot second group of Raw fluorescence
Image.
Intrinsic interval when being imaged due to image-carrying fiber bundle 10 between image transmission optical fibre monofilament 24 therein can be produced in imaging
Raw lattice, seriously affects picture quality and the interpretation to image information, even more can not carry out picture number to gained image
According to processing and quantitative analysis.So needing to handle Raw fluorescence image using image real time transfer control module 21.
Image real time transfer control module 21 reads two groups of Raw fluorescence images, uses respectively two groups of Raw fluorescence images
Gaussian filter algorithm carries out denoising, removes grid image caused by optical fiber interval in Raw fluorescence image, then pass through histogram
Figure equalization algorithm improves the contrast of fluorescent image, generates correction fluorescent image, and real-time synchronization shows institute over the display
The correction fluorescent image stated.
In the present embodiment, the Gaussian filter algorithm and algorithm of histogram equalization specifically include following steps：
Two groups of Raw fluorescence images are read respectively, with (- 1)x+yIt is multiplied by input picture to be coordinately transformed, utilizes discrete Fu
In leaf transformation calculate the DFT of image, be multiplied by F (μ, ν) with filter function H (μ, ν) and calculate anti-DFT, obtain result multiplied by with (-
1)x+yObtain the image after gaussian filtering.
The two dimensional form of gaussian filtering is as follows：
D (μ, ν) is blur radius r, wherein r in formula2=μ2+ν2；σ is the standard deviation of normal distribution.
When carrying out image procossing by algorithm of histogram equalization, specific mapping method is as follows：
Wherein n is the summation of pixel in image, nkIt is the number of pixels of current gray level grade, L is possible gray scale in image
Grade sum.
Gray value of image mapping can be obtained by above formula and the cell in fluorescence associated image is calculated by gray value of image
Nuclear volume, long term voyage, caryoplasm when quantitative parameters such as nuclear separation, and can gained image and related data be subjected to classification storage.
The fluorescence endoscope imaging system of the present embodiment can switch to different optical filters in light path, to meet different spies
For the requirement of filtering apparatus imaging, to realize to a variety of fluorescence probe fast imagings.In addition, due to each using Electronic control
The motor of runner can be switched with quick electric without opening camera bellows when switching, avoid the interference of external stray light while reduce
The loss of optical signalling in optical system.And due to having carried out image procossing, the quality of fluorescent image is improved, and can be right
Image carries out quantitative analysis.
Claims (8)
1. fluorescent endoscopic imgaing system, it is characterised in that：It is adopted including illuminating light source module, multispectral light splitting harvester, image
Collect processing module, mechano-electronic control module and for being placed in tissue to be detected to excite the biography of fluorescence probe and conducting image
As fiber optic bundle, the multispectral light splitting harvester is set in camera bellows and specifically includes exciter filter switch, dichroic
Mirror optical filtering switch and transmitting light optical-filter switcher, each switch include can along the pivot axis of itself runner,
Runner upper edge its be provided at circumferentially spaced it is multiple away from its pivot center apart from the identical optical port installed for respective filter, runner
On be additionally provided with for driving the runner along the actuator of the pivot axis of itself, the actuator is by mechano-electronic control
Molding block controls；Light path between illuminating light source module, exciter filter switch and dichroscope optical filtering switch, which is formed, to be used
In the first light path that lighting source is filtered into narrowband excitation light, the light between dichroscope optical filtering switch and image-carrying fiber bundle
Road forms the second light path, between dichroscope optical filtering switch, transmitting light optical-filter switcher and image acquisition and processing module
Light path forms the third light path of organization chart picture to be detected for rendering；The direction of second light path and third light path is on the contrary, first
Light path and the second light path and third light path are vertical.
2. fluorescent endoscopic imgaing system according to claim 1, it is characterised in that：In dichroscope in second light path
Enlarging objective module is provided between optical filtering switch and image-carrying fiber bundle, the enlarging objective module includes enlarging objective runner
And for drive amplification object lens runner on the enlarging objective runner actuator of its own pivot axis, enlarging objective runner
Multiple optical ports installed for enlarging objective are provided at circumferentially spaced along it, distance of multiple optical ports away from its pivot center is identical.
3. fluorescent endoscopic imgaing system according to claim 2, it is characterised in that：In enlarging objective in second light path
Optical alignment coupler is provided between module and image-carrying fiber bundle.
4. fluorescent endoscopic imgaing system according to claim 1, it is characterised in that：In lighting source in first light path
Hot spot adjuster is provided between module and exciter filter switch.
5. fluorescent endoscopic imgaing system according to any one of claims 1 to 4, it is characterised in that：The image transmission optical fibre
Beam includes more image transmission optical fibre monofilament and the image transmission optical fibre outer tube for being wrapped in bundles of image transmission optical fibre monofilament periphery.
6. fluorescent endoscopic imgaing system according to claim 5, it is characterised in that：Described image acquisition processing module includes
The image data that camera for receiving the transmitted image of image-carrying fiber bundle and the image for being received to camera are handled
Processing module, described image data processing module include for carrying out denoising to Raw fluorescence image to remove Raw fluorescence
The gaussian filtering module of grid image caused by image transmission optical fibre monofilament interval in image, image data processing module further includes integrating
There is algorithm of histogram equalization to improve the contrast of fluorescent image and generate the correction module of correction fluorescent image.
7. fluorescent endoscopic imgaing system according to claim 6, it is characterised in that：Described image data processing module also wraps
It includes for showing the correction fluorescent image, calculating quantitative parameter in correction fluorescent image and by gained image and related data
Carry out the server of classification storage.
8. fluorescent endoscopic imgaing system according to claim 6, it is characterised in that：The camera is to turn with image information
Change the CCD camera of unit, image information conversion unit is used to convert image information to data information.
Priority Applications (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110115557A (en) * | 2019-05-17 | 2019-08-13 | 中科光电(北京)科学技术有限公司 | A kind of EO-1 hyperion based endoscopic imaging device and imaging method |
| CN111122924A (en) * | 2018-10-31 | 2020-05-08 | 致茂电子(苏州)有限公司 | Probe alignment apparatus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111122924A (en) * | 2018-10-31 | 2020-05-08 | 致茂电子(苏州)有限公司 | Probe alignment apparatus |
| CN111122924B (en) * | 2018-10-31 | 2022-05-17 | 致茂电子(苏州)有限公司 | Probe alignment apparatus |
| CN110115557A (en) * | 2019-05-17 | 2019-08-13 | 中科光电(北京)科学技术有限公司 | A kind of EO-1 hyperion based endoscopic imaging device and imaging method |
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