CN102147523B - Biological digital microscope with double ccd (charge coupled device) light sensitive elements and photographic image processing method thereof - Google Patents
Biological digital microscope with double ccd (charge coupled device) light sensitive elements and photographic image processing method thereof Download PDFInfo
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Abstract
The invention discloses a biological digital microscope with double ccd (charge coupled device) light sensitive elements and a photographic image processing method thereof, wherein a colour ccd light sensitive element and a black and white ccd light sensitive element are used for collecting image information; the black and white ccd light sensitive element collects a gray scale and integrating optical density information of a specimen cell nucleus; the colour ccd light sensitive element collects a colour characteristic signal of a specimen image; the three-dimensional split joint of a specimen picture is performed by the gray scale of a specimen cell nucleus, the integrating optical density information and colour characteristic signal of a specimen image, so as to form a multi-focal plane holographic three-dimensional image; simultaneously, a film reading conclusion is output according to the integrating optical density information of a cell nucleus; finally a graphics context report of the specimen to be examined is output; therefore the biological digital microscope disclosed by the invention can perform conventional morphological observation for the specimen, as well as can perform digital photographing for the dyed DNA (deoxyribonucleic acid) for forming a multi-focal plane holographic three-dimensional image which is convenient for observing all the information of the cell in a multilayer and multi-focal plane mode; and the accuracy of early diagnosis is greatly improved.
Description
Technical field
The present invention relates to a kind of digital microscope, especially biological digital microscope of a kind of pair of ccd photo-sensitive cell and picked-up image processing method thereof are mainly used in the histotomy of microscopy through HE-Feulgen dyeing.
Background technology
Especially pathology development of medical development mainly relies on the amplification of optical microscope, thinks that doctor and scientific research personnel examine hardware foundation is provided the tissue specimen microcosmic of running business into particular one.In pathological research field and daily medical practice process, all there is growing demand for the application of processing, recorded and stored or the filing system of complex data and to the possibility of the mutual consulting of check result.
At present, state-of-the-art microscope just increases a utilizing camera interface simply outside eyepiece, play a part images acquired and throw on display.Nearly all medical pathologies microscopic system all is that the ccd photo-sensitive cell of only using a colour or black and white carries out work; Black and white ccd photo-sensitive cell has high sensitivity; Weak noise; Characteristics such as quantum efficiency be fit to the measurement of science data such as gray-scale value, integral optical density especially under low light conditions the analysis of the scientific analysis of micro-image such as dna have accurately characteristics such as quick; Colored ccd photo-sensitive cell be based on G the synthetic coloured image of three primary colors principle; If through colored ccd extract black white image only take G a passage in the triple channel handle; So can cause losing of view data; Therefore carry out gray analysis at the especially micro-pathological image of scientific research, its result often will distortion.Colored ccd possesses very desirable advantage again for the resolution and the isoparametric measurement of region area of color simultaneously.Because colored ccd is according to R, G, B three primary colors principle original image also; Its information judgement accurately is based on the differentiation of different colours; Consequently will lose 66% data message if distinguish half-tone information, but same black and white ccd can accomplish the accurate identification of gray scale can not distinguish the significant difference of different colours.Therefore, if having only a kind of ccd of form can not satisfy the needs of the full detail of micro-image collection.Carry out the collection of sample image information and reach and use the precision that black and white ccd photo-sensitive cell carries out gray analysis even the image processing software in later stage has how powerful all can not accomplish to use colored ccd photo-sensitive cell, perhaps utilize black and white ccd photo-sensitive cell to carry out the collection of sample image information and reach and use the sensitivity that colored ccd different colours is distinguished.Simultaneously, because pathological diagnosis is to be based upon under doctor's the prerequisite of experience, so-called pathological diagnosis goldstandard also is to be based upon under the prerequisite of individual experience, and it does not possess objective and standard.The long in addition also weary and inefficiency of microscopically work.In addition, medical institutions lack a large amount of doctors of pathology department also cause social medical resource especially the doctor of pathology department resource there is a serious shortage in the supply.
Summary of the invention
The present invention is directed to the deficiency of prior art; Provide a kind of pair of ccd photo-sensitive cell biological digital microscope, it carries out colored ccd photo-sensitive cell in same sample zone and black and white ccd photo-sensitive cell is taken pictures simultaneously, obtains 2 and is respectively black and white and colored image; Analysis through nuclear gray scale, integral optical density in the black white image obtains the DNA data characteristics in the nucleus; And set up sample central point to be checked, in conjunction with the color character in the cromogram, accomplish the splicing of complete sample.The present invention is effectively with the advantages of colored ccd photo-sensitive cell and black and white ccd photo-sensitive cell photographs; Therefore, the present invention not only can carry out normal observation, i.e. morphological observation to sample; Can also carry out digital photographing to DNA simultaneously through dyeing; Form many focal planes hologram three-dimensional image, be convenient to the full detail of multi-level, many focal planes ground observation of cell, improved the accuracy of early diagnosis widely.
For realizing above technical purpose, the present invention will take following technical scheme:
A kind of pair of biological digital microscope of ccd photo-sensitive cell; Comprise the sample image information harvester that frame and rack-mounted respectively micro-platform, MCU control chip, lens barrel, object lens are changed seat, are used to scan the bar code scanner of coded message on the microslide and are used to gather the microslide image information; Sample to be checked is undertaken in microslide; Said object lens conversion seat is gone up object lens is installed; Said object lens conversion seat, lens barrel, bar code scanner and sample image information harvester lay respectively at micro-platform top, and object lens conversion seat is connected with the lens barrel light path, and the light path of said object lens conversion exports the installation spectro-prism; Said lens barrel is the binocular lens barrel; Said sample image information harvester comprises black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell, and black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell are installed in respectively on the binocular lens barrel, and simultaneously black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell are respectively and be provided with an optical filtering between the Amici prism; Said MCU control chip comprises image information acquisition module, image information analysis and processing module and image information output module; The sample image color characteristic signal that sample nucleus gray scale, integral optical density information and the colored ccd photo-sensitive cell that image information acquisition module reception black and white ccd photo-sensitive cell is gathered gathered; The sample image color characteristic signal that the sample nucleus gray scale that the image information analysis and processing module is gathered according to black and white ccd photo-sensitive cell, integral optical density information and colored ccd photo-sensitive cell are gathered; Carry out the three-dimensional splicing of sample picture; Obtain many focal planes hologram three-dimensional image of sample to be checked, simultaneously read the sheet conclusion according to what integral optical density information and sample image color characteristic signal were made sample to be checked; The image information output module is according to the splice many focal planes hologram three-dimensional image that and read the sheet conclusion, the output picture and text report.
Another technical purpose of the present invention provides the picked-up image processing method of the biological digital microscope of a kind of above-mentioned pair of ccd photo-sensitive cell; May further comprise the steps: (1) image information collection adopts black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell respectively the image information collection to be carried out in the same sample zone of sample to be checked simultaneously; Black and white ccd photo-sensitive cell collection nuclear half-tone information of this sample to be checked and integral optical density information, colored ccd photo-sensitive cell is gathered the image color characteristic signal of this sample to be checked; (2) image information analyzing and processing A, according to R/G/B three primary colors principle, the image color characteristic signal that colored ccd photo-sensitive cell is gathered, the coloured image of the synthetic sample to be checked of splicing is to confirm the color character of this sample coloured image to be checked; The nucleus half-tone information and the integral optical density information of being gathered according to black and white ccd photo-sensitive cell are simultaneously set up the central point of sample to be checked, to obtain the nucleus DNA data characteristics of sample to be checked; B, the coloured image color character of the sample to be checked that is obtained in the A step and nucleus DNA data characteristics are combined; Accomplish the 3 D stereo splicing of sample to be checked; Obtain many focal planes hologram three-dimensional image of sample to be checked, simultaneously read the sheet conclusion according to what the nuclear integrated optical density information that is obtained was made sample to be checked; (3) image information output appends to the sheet conclusion of readding of the sample to be checked that obtains in the step (2) in corresponding many focal planes hologram three-dimensional image, exports the picture and text report of sample to be checked.
Further; In the B step of said step (2); The sheet conclusion of readding of making sample to be checked is specially: each institutional framework related information in the nuclear integrated optical density information of the sample to be checked that is obtained and the institutional framework standard information database is compared; Said institutional framework standard information database is set up each respective organization structon database according to the difference of institutional framework; And the related information of each institutional framework comprises nuclear integrated optical density information corresponding with it, sample image color characteristic information and institutional framework judgement conclusion in described each institutional framework subdata base; Said institutional framework judges that conclusion draws according to the nuclear integrated optical density information and the sample image color characteristic information of respective organization structure, if some institutional framework T of respective organization structon database in nuclear integrated optical density information, sample image color characteristic information and the institutional framework standard information database of sample to be checked
1Integral optical density information, sample image color characteristic information similarity greater than 90%, then automatically with institutional framework T
1Institutional framework judge that conclusion appends in many focal planes hologram three-dimensional image of sample to be checked, to generate the picture and text report of sample to be checked; If the nuclear integrated optical density information of sample to be checked and any the institutional framework T in the institutional framework standard information database
iThe similarity of integral optical density information less than 90%, perhaps any institutional framework T in the sample image color characteristic information of sample to be checked and the institutional framework standard information database
iThe similarity of sample image color characteristic information less than 90%; The capital automatically filters out this sample to be checked; Read sheet to carry out manual work, and manual work is read in many focal planes hologram three-dimensional image that the sheet conclusion appends to sample to be checked, to generate the picture and text report of sample to be checked; Simultaneously the sheet conclusion is read in nuclear integrated optical density information, sample image color characteristic information and the manual work of sample to be checked and store the corresponding organization structure subdata base together into, so that institutional framework standard information database is expanded/upgrades.
According to above technical scheme, can realize following beneficial effect:
The present invention adopts colored ccd photo-sensitive cell and black and white ccd photo-sensitive cell that the image information of sample is gathered simultaneously; The image information analysis and processing module of MCU control chip according to G the three primary colors principle; With the sample colour picture signal of being gathered; The synthetic sample coloured image of splicing is to confirm the color character of this coloured image; Simultaneously; Gray scale, the integral optical density information of the sample black white image that this image information analysis and processing module is gathered according to black and white ccd photo-sensitive cell are set up the sample central point, to obtain the data characteristics of sample; Color character in conjunction with aforementioned sample coloured image; Accomplish the splicing of sample 3 D stereo, simultaneously the optical density information of black and white picture is attached in the middle of the parameter of each cell, reach a conclusion through pathology expert's diagnosis; Dividing subset in view of the above, thus this process is set up database and is improved the purpose that software is able to intelligent screening repeatedly.Therefore: the present invention not only can carry out normal observation to sample; It is morphological observation; Can also carry out digital photographing to DNA simultaneously through dyeing; Form many focal planes hologram three-dimensional image, be convenient to the full detail of multi-level, many focal planes ground observation of cell, improved the accuracy of early diagnosis widely.The present invention is simple in structure, and is easy and simple to handle, and the microscopy degree of accuracy is high, has great guarantee for microscope inspection result's accuracy.
Description of drawings
Fig. 1 is a structural representation sketch of the present invention;
Fig. 2 is the process flow diagram of picked-up image processing method of the present invention;
Fig. 3 is the process flow diagram of image information analysis and processing module of the present invention;
Wherein: frame 1 micro-platform 2 slides are sheet device 3 object lens conversion seat 41 object lens 42 spectro-prisms 5 lens barrels 61 colored ccd photo-sensitive cell 62 black and white ccd photo-sensitive cells 63 up and down.
Embodiment
In order to make the public can fully understand technical spirit of the present invention and beneficial effect; To combine the accompanying drawing specific embodiments of the invention to describe in detail below; But the applicant is not the restriction to technical scheme to the description of embodiment, anyly makes form but not the variation of essence all should be regarded as protection scope of the present invention according to the present invention design.
As depicted in figs. 1 and 2; The of the present invention pair of biological digital microscope of ccd photo-sensitive cell; Comprise frame 1 and be installed in the sample image information harvester that micro-platform 2, MCU control chip, lens barrel 61, object lens on the frame 1 are changed seat 41, are used to scan the bar code scanner of coded message on the microslide and are used to gather the microslide image information respectively; Sample to be checked places on the microslide; On the said object lens conversion seat 41 object lens 42 are installed; Said object lens conversion seat 41, lens barrel 61, bar code scanner and sample image information harvester lay respectively at micro-platform 2 tops, and object lens conversion seat 41 is connected with lens barrel 61 light paths, and the light path of said object lens conversion seat 41 exports installation spectro-prism 5; Said lens barrel is the binocular lens barrel; Said sample image information harvester comprises black and white ccd photo-sensitive cell 63 and colored ccd photo-sensitive cell 62, and black and white ccd photo-sensitive cell 63 and colored ccd photo-sensitive cell 62 are installed in respectively on the binocular lens barrel, and simultaneously black and white ccd photo-sensitive cell 63 and colored ccd photo-sensitive cell 62 are respectively and be provided with an optical filtering between the Amici prism; Hence one can see that; The present invention adopts black and white ccd photo-sensitive cell 63 and colored ccd photo-sensitive cell 62 simultaneously the photography target of the same visual field to be made a video recording respectively; Therefore, there is not parallax in the data of the microslide that obtains, have greatly guaranteed the accuracy of image, text and data output; Said MCU control chip comprises image information acquisition module, image information analysis and processing module and image information output module; The sample image color characteristic signal that sample nucleus gray scale, integral optical density information and the colored ccd photo-sensitive cell 62 that image information acquisition module reception black and white ccd photo-sensitive cell 63 is gathered gathered; The sample image color characteristic signal that the sample nucleus gray scale that the image information analysis and processing module is gathered according to black and white ccd photo-sensitive cell 63, integral optical density information and colored ccd photo-sensitive cell 62 are gathered; Carry out the three-dimensional splicing of sample picture; Obtain many focal planes hologram three-dimensional image of sample to be checked, the image information output module is according to splice many focal planes hologram three-dimensional image of and nuclear integrated optical density information output picture and text report.Sample to be checked of the present invention is the histotomy through HE-Feulgen dyeing.The present invention is equipped with the led light source by MCU control chip control light intensity below the perform region; Behind this led light source process kohler's illumination system projects light beam; See through the sample to be checked on the microslide; After the light-splitting processing of the first time of object lens processing and amplifying, Amici prism, get into filter glass successively, shadow is projected respectively on colored ccd photo-sensitive cell 62 and the black and white ccd photo-sensitive cell 63; Take pictures with black and white ccd photo-sensitive cell 63 same sample zone is carried out simultaneously colored ccd photo-sensitive cell 62; Obtain 2 and be respectively black and white and colored image,, get final product the picture and text report of printout sample to be checked after the image information analysis and processing module of MCU control chip is handled.Wherein, microslide through Y three-dimensional move and make sample zone and thickness project to respectively by different level on colored ccd photo-sensitive cell 62 and the black and white ccd photo-sensitive cell 63.
Specifically; The picked-up image processing method of the according to the invention pair of biological digital microscope of ccd photo-sensitive cell; May further comprise the steps: (1) image information collection adopts black and white ccd photo-sensitive cell 63 and colored ccd photo-sensitive cell 62 respectively the image information collection to be carried out in the same sample zone of sample to be checked simultaneously; Black and white ccd photo-sensitive cell 63 is gathered nuclear half-tone information of this sample to be checked and integral optical density information, and colored ccd photo-sensitive cell 62 is gathered the image color characteristic signal of this sample to be checked; (2) image information analyzing and processing A, according to R/G/B three primary colors principle, the image color characteristic signal that colored ccd photo-sensitive cell 62 is gathered, the coloured image of the synthetic sample to be checked of splicing is to confirm the color character of this sample coloured image to be checked; The nuclear half-tone information of this sample to be checked and the integral optical density information of being gathered according to black and white ccd photo-sensitive cell 63 are simultaneously set up the central point of sample to be checked, to obtain the nucleus DNA data characteristics of sample to be checked; B, the coloured image color character of the sample to be checked that is obtained in the A step and nucleus DNA data characteristics are combined; Accomplish the 3 D stereo splicing of sample to be checked; Obtain many focal planes hologram three-dimensional image of sample to be checked, make the judgement conclusion of sample to be checked simultaneously according to the nuclear integrated optical density information that is obtained; (3) image information output is exported the picture and text report of sample to be checked according to the many focal planes hologram three-dimensional image and the judgement conclusion of the sample to be checked that obtains in the step (2).In addition; For realizing intelligent screening; Improve and read sheet efficient; In the B step of step according to the invention (2); The judgement conclusion of making sample to be checked is specially: each institutional framework related information in the nuclear integrated optical density information of the sample to be checked that is obtained and the institutional framework standard information database is compared; Said institutional framework standard information database is set up each respective organization structon database according to the difference of institutional framework; And the related information of each institutional framework comprises nuclear integrated optical density information corresponding with it, sample image color characteristic information and institutional framework judgement conclusion in described each institutional framework subdata base, and said institutional framework judges that conclusion draws according to the nuclear integrated optical density information and the sample image color characteristic information of respective organization structure, if some institutional framework T of respective organization structon database in nuclear integrated optical density information, sample image color characteristic information and the institutional framework standard information database of sample to be checked
1Integral optical density information, sample image color characteristic information similarity greater than 90%, then automatically with institutional framework T
1Institutional framework judge that conclusion appends in many focal planes hologram three-dimensional image of sample to be checked, to generate the picture and text report of sample to be checked; If the nuclear integrated optical density information of sample to be checked or any the institutional framework T in sample image color characteristic information and the institutional framework standard information database
iIntegral optical density information or sample image color characteristic information similarity all less than 90%; Then automatically filter out this sample to be checked; That is to say, as the nuclear integrated optical density information of sample to be checked and any the institutional framework T in the institutional framework standard information database
iThe similarity of integral optical density information less than 90%, perhaps as the sample image color characteristic information of sample to be checked and any the institutional framework T in the institutional framework standard information database
iThe similarity of sample image color characteristic information less than 90% o'clock; The capital automatically filters out sample to be checked; Read sheet to carry out manual work, and manual work is read in many focal planes hologram three-dimensional image that the sheet conclusion appends to sample to be checked, to generate the picture and text report of sample to be checked; Simultaneously the sheet conclusion is read in nuclear integrated optical density information, sample image color characteristic information and the manual work of sample to be checked and store the corresponding organization structure subdata base together into, so that institutional framework standard information database is expanded/upgrades.
Hence one can see that: what 1, black and white ccd photo-sensitive cell 63 was taken is nuclear picture in the dyeing histotomy; What be that its photographic subjects is directed against is the nucleus of each institutional framework; Half-tone information can be oriented nuclear position; Thereby can locate the central point of each institutional framework, and integral optical density information can quantitative exactly nuclear dna content; What colored ccd photo-sensitive cell 62 was taken is the peripheral complete image of each institutional framework in the dyeing histotomy, thereby the structure that can distinguish histotomy is formed; 2, the present invention preestablishes institutional framework standard information database in the MCU control chip; Then during microscopy; Earlier the integral optical density information of black and white ccd photo-sensitive cell 63 collections and the corresponding integral optical density information of each institutional framework in the institutional framework standard information database are compared; If satisfy certain condition; Then the judgement conclusion of pairing institutional framework in the institutional framework standard information database is exported automatically, with as sample to be checked read the sheet conclusion, thereby export the picture and text report of sample to be checked; If do not satisfy this condition; Then automatically sample to be checked is sifted out; Carry out manual work and read sheet, export the picture and text report of sample to be checked then, in addition; The nuclear integrated optical density information stores of also sheet conclusion and corresponding sample to be checked are read in manual work is to the institutional framework subdata base, so that institutional framework standard information database is expanded/upgrades.The present invention is auxiliary through institutional framework standard information database, automatically screens sample to be checked, has greatly practiced thrift manual work and has read the sheet cost.
The present invention carries out colored ccd photo-sensitive cell 62 and takes pictures simultaneously with black and white ccd photo-sensitive cell 63 in same sample zone; Obtain 2 and be respectively black and white and colored image; Analysis through nuclear gray scale, integral optical density in the black white image obtain in the nucleus data characteristics; And set up central point; In conjunction with the color character in the cromogram, (sample needs the photo more than 400 to accomplish, because adopt the ccd of 1/2 inch target surface to carry out 200-400 amplification system doubly to sample to reach on the target surface of ccd to accomplish complete sample; Therefore actual each quantity of information of opening on photo is 1/2 inch 200-400/1 on sample plane) splicing; Through being based upon nuclear central point parameter colour picture is carried out 3 D stereo splicing, simultaneously the cell optical density information of black and white picture is attached in the middle of the parameter of each cell, reach a conclusion through pathology expert's diagnosis; Dividing subset in view of the above, thus this process is set up database and is improved the purpose that software is able to intelligent screening repeatedly.
In sum, utilize colored ccd photo-sensitive cell 62 and black and white ccd photo-sensitive cell 63 to carry out the collection of sample image information simultaneously, accomplish the splicing of sample 3 D stereo, with the diagnosis of output sample.The present invention not only can carry out normal observation to sample; Be morphological observation, can also carry out digital photographing to DNA simultaneously, form many focal planes hologram three-dimensional image through dyeing; Be convenient to the full detail of multi-level, many focal planes ground observation of cell, improved the accuracy of early diagnosis widely.
Claims (3)
1. biological digital microscope of two ccd photo-sensitive cells; Comprise the sample image information harvester that frame and rack-mounted respectively micro-platform, MCU control chip, lens barrel, object lens are changed seat, are used to scan the bar code scanner of coded message on the microslide and are used to gather the microslide image information; Sample to be checked is undertaken in microslide; Said object lens conversion seat is gone up object lens is installed; Said object lens conversion seat, lens barrel, bar code scanner and sample image information harvester lay respectively at micro-platform top; And object lens conversion seat is connected with the lens barrel light path, it is characterized in that, spectro-prism is installed in the light path outlet of said object lens conversion seat; Said lens barrel is the binocular lens barrel; Said sample image information harvester comprises black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell, and black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell are installed in respectively on the binocular lens barrel, and simultaneously black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell are respectively and be provided with an optical filtering between the Amici prism; Said MCU control chip comprises image information acquisition module, image information analysis and processing module and image information output module; The sample image color characteristic signal that sample nucleus gray scale, integral optical density information and the colored ccd photo-sensitive cell that image information acquisition module reception black and white ccd photo-sensitive cell is gathered gathered; The sample image color characteristic signal that the sample nucleus gray scale that the image information analysis and processing module is gathered according to black and white ccd photo-sensitive cell, integral optical density information and colored ccd photo-sensitive cell are gathered; Carry out the three-dimensional splicing of sample picture; Obtain many focal planes hologram three-dimensional image of sample to be checked, simultaneously read the sheet conclusion according to what integral optical density information and sample image color characteristic signal were made sample to be checked; The image information output module is according to the splice many focal planes hologram three-dimensional image that and read the sheet conclusion, the output picture and text report.
2. the picked-up image processing method of the biological digital microscope of said pair of ccd photo-sensitive cell of a claim 1; It is characterized in that; May further comprise the steps: (1) image information collection: adopt black and white ccd photo-sensitive cell and colored ccd photo-sensitive cell respectively the image information collection to be carried out in the same sample zone of sample to be checked simultaneously; Black and white ccd photo-sensitive cell collection nuclear half-tone information of this sample to be checked and integral optical density information, colored ccd photo-sensitive cell is gathered the image color characteristic signal of this sample to be checked; (2) image information analyzing and processing: A, according to R/G/B three primary colors principle, the image color characteristic signal that colored ccd photo-sensitive cell is gathered, the coloured image of the synthetic sample to be checked of splicing is to confirm the color character of this sample coloured image to be checked; The nucleus half-tone information and the integral optical density information of being gathered according to black and white ccd photo-sensitive cell are simultaneously set up the central point of sample to be checked, to obtain the nucleus DNA data characteristics of sample to be checked; B, the coloured image color character of the sample to be checked that is obtained in the A step and nucleus DNA data characteristics are combined; Accomplish the 3 D stereo splicing of sample to be checked; Obtain many focal planes hologram three-dimensional image of sample to be checked; Simultaneously, read the sheet conclusion according to what the nuclear integrated optical density information that is obtained and sample image color characteristic signal were made sample to be checked; (3) image information output: the sheet conclusion of readding of the sample to be checked that obtains in the step (2) is appended in corresponding many focal planes hologram three-dimensional image, export the picture and text report of sample to be checked.
3. according to the picked-up image processing method of the biological digital microscope of the said pair of ccd photo-sensitive cell of claim 2; It is characterized in that; In the B step of said step (2); The sheet conclusion of readding of making sample to be checked is specially: each institutional framework related information in the nuclear integrated optical density information of the sample to be checked that is obtained and the institutional framework standard information database is compared; Said institutional framework standard information database is set up each respective organization structon database according to the difference of institutional framework; And the related information of each institutional framework comprises nuclear integrated optical density information corresponding with it, sample image color characteristic information and institutional framework judgement conclusion in described each institutional framework subdata base; Said institutional framework judges that conclusion draws according to the nuclear integrated optical density information and the sample image color characteristic information of respective organization structure, if some institutional framework T of respective organization structon database in nuclear integrated optical density information, sample image color characteristic information and the institutional framework standard information database of sample to be checked
1Integral optical density information, sample image color characteristic information similarity greater than 90%, then automatically with institutional framework T
1Institutional framework judge that conclusion appends in many focal planes hologram three-dimensional image of sample to be checked, to generate the picture and text report of sample to be checked; If the nuclear integrated optical density information of sample to be checked and any the institutional framework T in the institutional framework standard information database
iThe similarity of integral optical density information less than 90%, perhaps any institutional framework T in the sample image color characteristic information of sample to be checked and the institutional framework standard information database
iThe similarity of sample image color characteristic information less than 90%; The capital automatically filters out this sample to be checked; Read sheet to carry out manual work, and manual work is read in many focal planes hologram three-dimensional image that the sheet conclusion appends to sample to be checked, to generate the picture and text report of sample to be checked; Simultaneously the sheet conclusion is read in nuclear integrated optical density information, sample image color characteristic information and the manual work of sample to be checked and store the corresponding organization structure subdata base together into, institutional framework standard information database is expanded or upgrade.
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