CN110292001A - A kind of cultural method improving Tilapia mossambica immunity - Google Patents
A kind of cultural method improving Tilapia mossambica immunity Download PDFInfo
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- CN110292001A CN110292001A CN201910402012.XA CN201910402012A CN110292001A CN 110292001 A CN110292001 A CN 110292001A CN 201910402012 A CN201910402012 A CN 201910402012A CN 110292001 A CN110292001 A CN 110292001A
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- 241000276701 Oreochromis mossambicus Species 0.000 title claims abstract description 77
- 230000036039 immunity Effects 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 80
- 238000002156 mixing Methods 0.000 claims abstract description 32
- 239000012535 impurity Substances 0.000 claims abstract description 24
- 235000011194 food seasoning agent Nutrition 0.000 claims abstract description 14
- 230000007480 spreading Effects 0.000 claims abstract description 14
- 238000003892 spreading Methods 0.000 claims abstract description 14
- 239000003814 drug Substances 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 34
- 238000001914 filtration Methods 0.000 claims description 28
- 238000011049 filling Methods 0.000 claims description 15
- 239000000654 additive Substances 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 13
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 13
- 238000011534 incubation Methods 0.000 claims description 12
- 230000008054 signal transmission Effects 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 244000063299 Bacillus subtilis Species 0.000 claims description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 11
- 238000007726 management method Methods 0.000 claims description 11
- 235000013557 nattō Nutrition 0.000 claims description 11
- 244000057717 Streptococcus lactis Species 0.000 claims description 10
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 239000002131 composite material Substances 0.000 claims description 10
- 230000010355 oscillation Effects 0.000 claims description 10
- 241000235342 Saccharomycetes Species 0.000 claims description 9
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 9
- 239000011707 mineral Substances 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 235000013717 Houttuynia Nutrition 0.000 claims description 8
- 240000000691 Houttuynia cordata Species 0.000 claims description 8
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 8
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 8
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 8
- 241000108664 Nitrobacteria Species 0.000 claims description 7
- 238000007667 floating Methods 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 7
- 239000013535 sea water Substances 0.000 claims description 7
- 230000002421 anti-septic effect Effects 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 241000252230 Ctenopharyngodon idella Species 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 241000252234 Hypophthalmichthys nobilis Species 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 244000269722 Thea sinensis Species 0.000 claims description 3
- 241000276707 Tilapia Species 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 239000006260 foam Substances 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 241000235575 Mortierella Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000006071 cream Substances 0.000 claims 1
- 239000010813 municipal solid waste Substances 0.000 claims 1
- 244000144977 poultry Species 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
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- 241000251468 Actinopterygii Species 0.000 description 4
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- 229940079593 drug Drugs 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000907999 Mortierella alpina Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
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- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000003911 water pollution Methods 0.000 description 2
- 208000003322 Coinfection Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/80—Feeding devices
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/045—Filters for aquaria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23N—MACHINES OR APPARATUS FOR TREATING HARVESTED FRUIT, VEGETABLES OR FLOWER BULBS IN BULK, NOT OTHERWISE PROVIDED FOR; PEELING VEGETABLES OR FRUIT IN BULK; APPARATUS FOR PREPARING ANIMAL FEEDING- STUFFS
- A23N17/00—Apparatus specially adapted for preparing animal feeding-stuffs
- A23N17/007—Apparatus specially adapted for preparing animal feeding-stuffs for mixing feeding-stuff components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
- B01D35/02—Filters adapted for location in special places, e.g. pipe-lines, pumps, stop-cocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/05—Stirrers
- B01F27/11—Stirrers characterised by the configuration of the stirrers
- B01F27/19—Stirrers with two or more mixing elements mounted in sequence on the same axis
- B01F27/191—Stirrers with two or more mixing elements mounted in sequence on the same axis with similar elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D21/00—Measuring or testing not otherwise provided for
- G01D21/02—Measuring two or more variables by means not covered by a single other subclass
-
- G—PHYSICS
- G08—SIGNALLING
- G08C—TRANSMISSION SYSTEMS FOR MEASURED VALUES, CONTROL OR SIMILAR SIGNALS
- G08C17/00—Arrangements for transmitting signals characterised by the use of a wireless electrical link
- G08C17/02—Arrangements for transmitting signals characterised by the use of a wireless electrical link using a radio link
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/60—Fishing; Aquaculture; Aquafarming
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- Life Sciences & Earth Sciences (AREA)
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- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Polymers & Plastics (AREA)
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- Zoology (AREA)
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- Physics & Mathematics (AREA)
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- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
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- Fodder In General (AREA)
Abstract
The invention belongs to Tilapia mossambica cultural technique fields, disclose a kind of cultural method for improving Tilapia mossambica immunity, including the Chinese medicine and nutriment that improve immunity are mixed, fermented, feed after mixing, fermentation is put into feed mixing chamber, indoor agitator arm is mixed by feed, feed is stirred；Feed mixing chamber is put into water body, carries out driving movement to whole by the implementation fan of rear end；By being filtered inside filter device, by water it is intracorporal kill and devour, excrement stays in filter cartridge, circulation removal of impurities is carried out to water body；Feed is mixed indoor feed by seasoning spreading box to be sprinkled into water body, feeding is carried out to Tilapia mossambica, to guarantee that Tilapia mossambica can eat with the fresh feed for improving immunity.The present invention is by feeding and removal of impurities together as one, while facilitating poultry raiser to carry out feeding to Tilapia mossambica, can also handle at any time the removal of impurities that water body carries out, purify water body environment, can be improved the immunity of Tilapia mossambica.
Description
Technical field
The invention belongs to Tilapia mossambica cultural technique field more particularly to a kind of cultural methods for improving Tilapia mossambica immunity.
Background technique
It currently, Tilapia mossambica is the main breed variety of world's aquatic products industry, while being also the freshwater aquiculture of emphasis scientific research culture
Fish, and it is known as one of the main source of the following animal protein.The meat flavour of Tilapia mossambica is delicious, fine and tender taste, containing more
Kind unsaturated fatty acid and protein abundant, are favored by people.It is rich in water but since water pollution was more serious in recent years
The problems such as nutrient laden, is more prominent, and water body environment virus increases, and has seriously affected the growth of Tilapia mossambica, has increased the hair of Tilapia mossambica
Sick rate causes disease in Tilapia mossambica breeding process in recent years to take place frequently.Simultaneously as Tilapia mossambica disease, which takes place frequently, to be caused in breeding process
Drug investment increasingly increases, and the drug of these investments may be enriched with food, further be passed to by food chain edible
The mankind of Tilapia mossambica may generate serious harm to human health.
By fish culture, numerous species are successfully introduced new region.In fish culture prepared by benefit-additives
If temperature is excessive in journey, it is easy to cause additive preparation failure；It will cause harm simultaneously.
In conclusion problem of the existing technology is:
It is more prominent the problems such as eutrophication in water but since water pollution was more serious in recent years, water body environment virus
Increase, has seriously affected the growth of Tilapia mossambica, increased the disease incidence of Tilapia mossambica, led to disease in Tilapia mossambica breeding process in recent years
Disease takes place frequently.Drug investment in breeding process is caused increasingly to increase simultaneously as Tilapia mossambica disease takes place frequently, and the drug of these investments
It may be enriched with food, the mankind of edible Tilapia mossambica are further passed to by food chain, human health may generated tight
It endangers again.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of cultural methods for improving Tilapia mossambica immunity.
The invention is realized in this way a kind of cultural method for improving Tilapia mossambica immunity, the raising Tilapia mossambica are immune
Described in the cultural method of power improve Tilapia mossambica immunity cultural method include:
The first step carries out sterilization of cleaning up the pond to cultivating pool before putting Tilapia mossambica in a suitable place to breed；Cultivating pool is drained into pool water first, cruelly
It shines 8 days；Secondly a small amount of pool water of injection, and be uniformly sprinkled into pool water and beat subsequent tea bran foam；After impregnating 5 days, pool water is drained, 3
Clean water is refilled after it, to cultivating tilapia；
Second step makes cordate houttuynia floating bed, and applies fertilizer into water body, cultivates plankton bait；By the mineral spring of 500mL
Water bottle is cut, and 1/2-the 2/3 of bottom of bottle bottom end is left and taken, and is uniformly perforated in bottom, the mineral water bottle having openning hole is inserted into square
In the latticed brandreth of shape, using hollow pvc pipe closed at both ends, it is fixed below brandreth, carries out buoyancy support；By 12cm and band
There is axillary-bud or top-bud cordate houttuynia seedling to be inserted into mineral water bottle, floating bed is neatly distributed in pond surrounding after the completion of cuttage；
Third step is embathed Tilapia mossambica 5-10 minutes using the saline solution of 3%-5% before launching；
4th step is launched appropriate bighead, Silver Carp, grass carp and Tilapia mossambica into water body and is raised together；
5th step improves Tilapia mossambica immunity by daily management.
Further, the 5th step, the method by daily management raising Tilapia mossambica immunity further comprises: benefit-additives
It is added with daily feed 1:2000 by weight；Benefit-additives are by weight by 2 parts of bafillus natto, bacillus subtilis
2 parts, 2 parts of saccharomycete, 2 parts of streptococcus lactis, 1 part of lactobacillus acidophilus, 2 parts of saccharomyces cerevisiae and 0.5 part of nitrobacteria composition；More than
Bacterium is all from commercially available.
By bafillus natto, bacillus subtilis, saccharomycete, streptococcus lactis, lactobacillus acidophilus, saccharomyces cerevisiae, nitre
Change bacterium to carry out being inoculated in slant medium respectively；
Strain is inoculated in respectively in the conical flask for filling fluid nutrient medium, culture medium is prepared with antiseptic sea water, and pH is
7.5；Conical flask is put into shaking table oscillation, and temperature is 30 DEG C, vibrates 10 hours；It is counted using blood counting chamber, strain number in bacterium solution
Amount respectively reaches 108A/mL；
Mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium and is fermented.
Bafillus natto cultivates 20h at 30 DEG C, and bacillus subtilis cultivates 35h at 30 DEG C, at 30 DEG C of saccharomycete
Culture 40h obtains a ring activated spawn；Streptococcus lactis cultivation temperature is 30 DEG C, incubation time 10h；Saccharomyces cerevisiae culture temperature
Degree is 30 DEG C, incubation time 12h；Mortierella alpina cultivation temperature is 30 DEG C, incubation time 10h；Nitrobacteria culture temperature
Degree is 30 DEG C, incubation time 15h；
When being fermented in fermentor, strain is inoculated in respectively in the conical flask for filling fluid nutrient medium, culture medium is used
Antiseptic sea water is prepared, pH6.5；
It is put into shaking table oscillation, temperature is 30 DEG C, and oscillation for 24 hours, makes strain quantity respectively reach 108A/mL presses each strain
The mass percent of the strain mixes；
Mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium, composite bacteria agent medium pH 6.5,
Inoculum concentration is 0.5%, cultivates 2 days at 30 DEG C, bacterium number in fermentation liquid is made to reach 3~5 × 108A/mL, obtains benefit-additives.
Further, the daily management method of the 5th step specifically includes:
(1) Chinese medicine for improving immunity and nutriment mixed, fermented, the feed after mixing, fermentation is put into
In feed mixing chamber, indoor agitator arm is mixed by feed, feed is stirred；
(2) feed mixing chamber is put into water body, driving movement is carried out to whole by the implementation fan of rear end, by pushing away for two sides
Row fan maintains whole balance；
(3) water flow is drawn into inside filter device by pulsating pump and is filtered, by water it is intracorporal kill and devour, excrement stays in
In filter cartridge, the water after removal of impurities is discharged into water body again by filtering rear cover, carries out circulation removal of impurities to water body；
(4) feed is mixed indoor feed by seasoning spreading box to be sprinkled into water body, feeding is carried out to Tilapia mossambica, guarantees Rofe
Fish can eat with the fresh feed for improving immunity.
Another object of the present invention is to provide a kind of cultivation apparatus for improving Tilapia mossambica immunity, the raising Tilapia mossambicas
The cultivation apparatus of immunity is provided with shell, and feed mixing chamber is offered among enclosure interior, and housing rear end is fanned embedded with implementation,
Housing forward end be it is coniform, control room is offered inside housing forward end, single-chip microcontroller, GPRS have been screwed inside control room
Signal transmission device, GPS locator；
Shell lower end center weldment has connecting rod, and connecting rod lower end is welded with filter device, there is bolt inside filter device
Filtering and impurity removing device, filtering and impurity removing device are connected to by pulsating pump with the water inlet pipe of filter device front end, filtering and impurity removing device be welded on
The filter cartridge of filter device rear end is connected to；
Enclosure interior is fixed with battery on the right side of feed mixing chamber, and battery and single-chip microcontroller fan driving electricity with implementation
Machine, GPRS signal transmission device, GPS locator, pulsating pump electrical connection.
Further, feed mixing chamber interior lower end is equipped with rotating electric machine by screw, and rotating electric machine upper end is welded with more
A agitator arm, rotating electric machine and battery, monolithic mechatronics, feed mixing chamber upper end offers feed opening, feed opening card
Equipped with feeding cover, sealing rubber ring is enclosed on the outside of feeding cover.
Further, housing rear end two sides are embedded with steering fan, and the steering fan position of two sides is symmetrical, steering fan driving electricity
Machine is electrically connected with single-chip microcontroller, battery.
Further, control room front end two sides are embedded with ultrasonic sensor, ultrasonic sensor and single-chip microcontroller, battery
Electrical connection.
Further, shell lower end two sides are embedded with seasoning spreading box, and seasoning spreading box upper end offers discharge funnel, discharge funnel upper end
Opening is connected to feed mixing chamber, and discharge roller, the discharge electricity of discharge roller and side are embedded at discharge funnel lower ending opening
Arbor connection, discharge motor are electrically connected with single-chip microcontroller, battery.
Further, filter cartridge is cylindrical closed vessel, filtering rear cover has been bolted on rear side of filter cartridge, after filtering
Lid filter opening diameter is less than the diameter of general sundries, and filtering rear cover front end is filled with sponge, is filled with active carbon particle in filter cartridge.
Advantages of the present invention and good effect are as follows: the present invention carries out intelligent control to whole driving device by single-chip microcontroller,
Preliminary purification can be carried out to water body environment by filtering and impurity removing device, improve the cleanliness of water body environment；Agitator arm can
Uniform stirring is carried out to the feed for improving immunity to launch feed by seasoning spreading box under the premise of removal of impurities, it can be true
It protects Tilapia mossambica and eats the fresh feed just launched, can be connect with external control centre by GPRS signal transmission device, realization pair
The long-range control of single unit system, can be positioned by GPS locator, avoid water surface area too big, can not determining device
Location can detect barrier in water by ultrasonic sensor, prevent that touching is caused to damage, influence to make
With.
The present invention is by feeding and removal of impurities together as one, also can be with while facilitating poultry raiser to carry out feeding to Tilapia mossambica
When to water body carry out removal of impurities handle, purified water body environment, can be improved the immunity of Tilapia mossambica.
The present invention is disinfected before putting Tilapia mossambica in a suitable place to breed to putting pond in a suitable place to breed, drastically reduce noxious material with
Virulence factor improves water quality；Cordate houttuynia floating bed has been built in the cultivating pool for putting Tilapia mossambica in a suitable place to breed, can significantly improve Rofe
The immunity and growth performance of fish absorb, nitrogen, phosphorus and organic matter superfluous in assimilation pond, improve water quality；Put Rofe in a suitable place to breed simultaneously
It is sterilized before fish, avoids bacterium secondary infection caused by damaging because of launch process；Big head, grass carp and Rofe simultaneously
Fish mixed breeding reasonably can reduce waste using resource, improve income.
The present invention further comprises by the method that daily management improves Tilapia mossambica immunity: benefit-additives and daily feeding
Expect the addition of 1:2000 by weight；Benefit-additives are by weight by 2 parts of bafillus natto, 2 parts of bacillus subtilis, yeast
2 parts of bacterium, 2 parts of streptococcus lactis, 1 part of lactobacillus acidophilus, 2 parts of saccharomyces cerevisiae and 0.5 part of nitrobacteria composition；The above bacterium is all from
It is commercially available.
By bafillus natto, bacillus subtilis, saccharomycete, streptococcus lactis, lactobacillus acidophilus, saccharomyces cerevisiae, nitre
Change bacterium to carry out being inoculated in slant medium respectively；
Strain is inoculated in respectively in the conical flask for filling fluid nutrient medium, culture medium is prepared with antiseptic sea water, and pH is
7.5；Conical flask is put into shaking table oscillation, and temperature is 30 DEG C, vibrates 10 hours；It is counted using blood counting chamber, strain number in bacterium solution
Amount respectively reaches 108A/mL；Mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium and is fermented.
Obtain benefit-additives.It can be guaranteed for the raising of Tilapia mossambica immunity.
Detailed description of the invention
Fig. 1 is the cultural method flow chart provided in an embodiment of the present invention for improving Tilapia mossambica immunity.
Fig. 2 is the daily management method flow diagram provided in an embodiment of the present invention for improving Tilapia mossambica immunity.
Fig. 3 is the cultivation apparatus structural schematic diagram provided in an embodiment of the present invention for improving Tilapia mossambica immunity；
Fig. 4 is feed mixing chamber structural schematic diagram provided in an embodiment of the present invention；
Fig. 5 is single chip circuit figure provided in an embodiment of the present invention；
Fig. 6 is GPRS signal transmission device circuit diagram provided in an embodiment of the present invention；
In figure: 1, shell；2, feed mixing chamber；3, control room；4, carry out fan；5, single-chip microcontroller；6, GPRS signal transmission device；
7, GPS locator；8, connecting rod；9, filter device；10, filtering and impurity removing device；11, pulsating pump；12, water inlet pipe；13, filter cartridge；
14, battery；15, rotating electric machine；16, agitator arm；17, steering fan；18, ultrasonic sensor；19, seasoning spreading box；20, discharge
Funnel；21, discharge roller；22, discharge motor；23, rear cover is filtered；24, feeding cover.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
Structure of the invention is explained in detail with reference to the accompanying drawing.
As shown in Figure 1, the cultural method provided in an embodiment of the present invention for improving Tilapia mossambica immunity includes:
S101: sterilization of cleaning up the pond is carried out to cultivating pool before putting Tilapia mossambica in a suitable place to breed；
S102: production cordate houttuynia floating bed, and apply fertilizer into water body, cultivate plankton bait；
S103: Tilapia mossambica is embathed 5-10 minutes or so using the saline solution of 3%-5% before launching；
S104: appropriate bighead, Silver Carp, grass carp and Tilapia mossambica are launched into water body and is raised together；
S105: Tilapia mossambica immunity is further increased by daily management.
In step S101, sterilization provided in an embodiment of the present invention of cleaning up the pond is specifically included:
Cultivating pool is drained into pool water first, is exposed to the sun 8 days；Secondly a small amount of pool water of injection, and be uniformly sprinkled into and beat into pool water
Subsequent tea bran foam；After impregnating 5 days, pool water is drained, clean water is refilled after 3 days, to cultivating tilapia.
In step S102, cordate houttuynia floating bed production method provided in an embodiment of the present invention is specifically included:
The mineral water bottle of 500mL is cut, 1/2-the 2/3 of bottom of bottle bottom end is left and taken, uniformly perforates, will beat in bottom
In the mineral water bottle insertion square net shape brandreth in good hole, using hollow pvc pipe closed at both ends, it is fixed below brandreth,
Carry out buoyancy support；It is inserted into mineral water bottle by 12cm or so and with axillary-bud or top-bud cordate houttuynia seedling, will be floated after the completion of cuttage
Bed is neatly distributed in pond surrounding.
As shown in Fig. 2, the daily management method provided in an embodiment of the present invention for improving Tilapia mossambica immunity specifically includes:
The Chinese medicine for improving immunity and nutriment are mixed, are fermented by S201, and the feed after mixing, fermentation is put
Enter in feed mixing chamber, indoor agitator arm is mixed by feed, feed is stirred；
Feed mixing chamber is put into water body by S202, driving movement is carried out to whole by the implementation fan of rear end, by two sides
Carry out the balance that fan remains whole；
Water flow is drawn into inside filter device by pulsating pump and is filtered by S203, by water it is intracorporal kill and devour, excrement stays
In filter cartridge, the water after removal of impurities is discharged into water body again by filtering rear cover, carries out circulation removal of impurities to water body；
Feed is mixed indoor feed by seasoning spreading box and is sprinkled into water body by S204, feeding is carried out to Tilapia mossambica, to protect
Card Tilapia mossambica can eat with the fresh feed for improving immunity.
The present invention further comprises by the method that daily management improves Tilapia mossambica immunity: benefit-additives and daily feeding
Expect the addition of 1:2000 by weight；Benefit-additives are by weight by 2 parts of bafillus natto, 2 parts of bacillus subtilis, yeast
2 parts of bacterium, 2 parts of streptococcus lactis, 1 part of lactobacillus acidophilus, 2 parts of saccharomyces cerevisiae and 0.5 part of nitrobacteria composition；The above bacterium is all from
It is commercially available.
By bafillus natto, bacillus subtilis, saccharomycete, streptococcus lactis, lactobacillus acidophilus, saccharomyces cerevisiae, nitre
Change bacterium to carry out being inoculated in slant medium respectively；
Strain is inoculated in respectively in the conical flask for filling fluid nutrient medium, culture medium is prepared with antiseptic sea water, and pH is
7.5；Conical flask is put into shaking table oscillation, and temperature is 30 DEG C, vibrates 10 hours；It is counted using blood counting chamber, strain number in bacterium solution
Amount respectively reaches 108A/mL；
Mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium and is fermented.
Bafillus natto cultivates 20h at 30 DEG C, and bacillus subtilis cultivates 35h at 30 DEG C, at 30 DEG C of saccharomycete
Culture 40h obtains a ring activated spawn；Streptococcus lactis cultivation temperature is 30 DEG C, incubation time 10h；Saccharomyces cerevisiae culture temperature
Degree is 30 DEG C, incubation time 12h；Mortierella alpina cultivation temperature is 30 DEG C, incubation time 10h；Nitrobacteria culture temperature
Degree is 30 DEG C, incubation time 15h；
When being fermented in fermentor, strain is inoculated in respectively in the conical flask for filling fluid nutrient medium, culture medium is used
Antiseptic sea water is prepared, pH6.5；
It is put into shaking table oscillation, temperature is 30 DEG C, and oscillation for 24 hours, makes strain quantity respectively reach 108A/mL presses each strain
The mass percent of the strain mixes；
Mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium, composite bacteria agent medium pH 6.5,
Inoculum concentration is 0.5%, cultivates 2 days at 30 DEG C, bacterium number in fermentation liquid is made to reach 3~5 × 108A/mL, obtains benefit-additives.
As shown in Figures 3 to 6, the cultivation apparatus provided in an embodiment of the present invention for improving Tilapia mossambica immunity includes: shell
1, feed mixing chamber 2, control room 3, implementation fan 4, single-chip microcontroller 5, GPRS signal transmission device 6, GPS locator 7, connecting rod 8, filtering
Device 9, filtering and impurity removing device 10, pulsating pump 11, water inlet pipe 12, filter cartridge 13, battery 14, rotating electric machine 15, agitator arm 16,
Steering fan 17, ultrasonic sensor 18, seasoning spreading box 19, discharge funnel 20, discharge roller 21, discharge motor 22, filtering rear cover 23,
Feeding cover 24.
1 bosom of shell offers feed mixing chamber 2, and 1 rear end of shell is circular cone embedded with fan 4,1 front end of shell is carried out
Shape offers control room 3 inside 1 front end of shell, single-chip microcontroller 5, GPRS signal transmission device has been screwed inside control room 3
6, GPS locator 7；1 lower end center weldment of shell has connecting rod 8, and 8 lower end of connecting rod is welded with filter device 9, filter device 9
Inside bolt has filtering and impurity removing device 10, and filtering and impurity removing device 10 is connected to by pulsating pump 11 with the water inlet pipe 12 of 9 front end of filter device,
Filtering and impurity removing device 10 is connected to the filter cartridge 13 for being welded on 9 rear end of filter device；It is solid on the right side of feed mixing chamber 2 inside shell 1
Surely have a battery 14, battery 14 and single-chip microcontroller 5 with carry out 4 driving motors of fan, GPRS signal transmission device 6, GPS locator 7,
Pulsating pump 11 is electrically connected.
Further, 2 interior lower end of feed mixing chamber is equipped with rotating electric machine 15, the welding of 15 upper end of rotating electric machine by screw
There are multiple agitator arms 16, rotating electric machine 15 is electrically connected with battery 14, single-chip microcontroller 5, and 2 upper end of feed mixing chamber offers charging
Mouthful, feed opening is arranged with feeding cover 24, is enclosed with sealing rubber ring on the outside of feeding cover 24.
Further, 1 rear end sides of shell are embedded with steering fan 17, and 17 position of steering fan of two sides is symmetrical, steering fan
17 driving motors are electrically connected with single-chip microcontroller 5, battery 14.
Further, 3 front end two sides of control room are embedded with ultrasonic sensor 18, ultrasonic sensor 18 and single-chip microcontroller 5,
Battery 14 is electrically connected.
Further, 1 lower end two sides of shell are embedded with seasoning spreading box 19, and 19 upper end of seasoning spreading box offers discharge funnel 20, discharge
20 upper end opening of funnel is connected to feed mixing chamber 2, and discharge roller 21, discharge roller are embedded at 20 lower ending opening of discharge funnel
21 are electrically connected with 22 axis connection of discharge motor of side, discharge motor 22 with single-chip microcontroller 5, battery 14.
Further, filter cartridge 13 is cylindrical closed vessel, and filtering rear cover 23 has been bolted on rear side of filter cartridge 13,
The diameter that 23 filter opening diameter of rear cover is less than general sundries is filtered, filtering 23 front end of rear cover is filled with sponge, filling in filter cartridge 13
Active charcoal particle.
Preferably, GPS locator 7 uses SIM28M, ultrasonic sensor 18 uses GH311.
The present invention can send control instruction to single-chip microcontroller 5 by GPRS signal transmission device 6 by external control terminal, real
Intelligent control now is carried out to whole driving device, feeding cover 24 is opened when use, and that pre-coordination is added into feed mixing chamber 2 is good
The Chinese medicine and mixture of nutrients that can be improved Tilapia mossambica immunity, close feeding cover 24, pass through single-chip microcontroller 5 control rotation
Motor 15 rotates, and agitator arm 16 is driven to carry out Stirring；
The control of single-chip microcontroller 5 carries out fan 4 and is moved single unit system drive under water, is drawn into water flow by pulsating pump 11
Filtering and impurity removing device 10 can carry out preliminary purification to water body environment, improve the cleanliness of water body environment；
Single-chip microcontroller 5 controls discharge motor 22 and rotates, and 2 materials inside of feed mixing chamber is taken in the rotation of discharge roller 21 out of, leads to
It crosses seasoning spreading box to launch feed, it can be ensured that Tilapia mossambica eats the fresh feed just launched, and passes through GPRS signal transmission device 6
It can be connect with external control centre, realize the long-range control to single unit system, can be positioned by GPS locator 7, and
Location information is transmitted to controlling terminal, avoids water surface area too big, can not the location of determining device, pass through ultrasonic wave
Sensor 18 can detect barrier in water, prevent from causing touching to damage, influence using.
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (10)
1. a kind of cultural method for improving Tilapia mossambica immunity, which is characterized in that the cultivation side for improving Tilapia mossambica immunity
Described in method improve Tilapia mossambica immunity cultural method include:
The first step carries out sterilization of cleaning up the pond to cultivating pool before putting Tilapia mossambica in a suitable place to breed；Cultivating pool is drained into pool water first, is exposed to the sun 8
It；Secondly a small amount of pool water of injection, and be uniformly sprinkled into pool water and beat subsequent tea bran foam；After impregnating 5 days, pool water is drained, after 3 days
Clean water is refilled, to cultivating tilapia；
Second step makes cordate houttuynia floating bed, and applies fertilizer into water body, cultivates plankton bait；By the mineral water bottle of 500mL
It is cut, leaves and takes 1/2-the 2/3 of bottom of bottle bottom end, uniformly perforated in bottom, the mineral water bottle having openning hole is inserted into square net
In trellis brandreth, using hollow pvc pipe closed at both ends, it is fixed below brandreth, carries out buoyancy support；By 12cm and have armpit
Bud or terminal bud cordate houttuynia seedling are inserted into mineral water bottle, and floating bed is neatly distributed in pond surrounding after the completion of cuttage；
Third step is embathed Tilapia mossambica 5-10 minutes using the saline solution of 3%-5% before launching；
4th step is launched appropriate bighead, Silver Carp, grass carp and Tilapia mossambica into water body and is raised together；
5th step improves Tilapia mossambica immunity by daily management.
2. improving the cultural method of Tilapia mossambica immunity as described in claim 1, which is characterized in that the 5th step, by daily
The method that management improves Tilapia mossambica immunity further comprises: benefit-additives and daily feed 1:2000 by weight are added；
Benefit-additives by weight by 2 parts of bafillus natto, 2 parts of bacillus subtilis, 2 parts of saccharomycete, 2 parts of streptococcus lactis,
1 part of lactobacillus acidophilus, 2 parts of saccharomyces cerevisiae and 0.5 part of nitrobacteria composition；
Bafillus natto, bacillus subtilis, saccharomycete, streptococcus lactis, lactobacillus acidophilus, saccharomyces cerevisiae, nitrification is thin
Bacterium carries out being inoculated in slant medium respectively；
Strain is inoculated in respectively in the conical flask for filling fluid nutrient medium, culture medium is prepared with antiseptic sea water, pH 7.5；Cone
Shape bottle is put into shaking table oscillation, and temperature is 30 DEG C, vibrates 10 hours；It is counted using blood counting chamber, strain quantity is distinguished in bacterium solution
Reach 108A/mL；
Mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium and is fermented.
3. such as the cultural method for the raising Tilapia mossambica immunity that claim 2 is stated, which is characterized in that bafillus natto is at 30 DEG C
Lower culture 20h, bacillus subtilis cultivate 35h at 30 DEG C, and culture 40h obtains a ring activated spawn at 30 DEG C of saccharomycete；Cream
Acid streptococci cultivation temperature is 30 DEG C, incubation time 10h；Saccharomyces cerevisiae cultivation temperature is 30 DEG C, incubation time 12h；It is high
Mountain Mortierella cultivation temperature is 30 DEG C, incubation time 10h；Nitrobacteria cultivation temperature is 30 DEG C, incubation time 15h；
When being fermented in fermentor, strain is inoculated in respectively in the conical flask for filling fluid nutrient medium, culture medium is with sterile
Seawater is prepared, pH6.5；
It is put into shaking table oscillation, temperature is 30 DEG C, and oscillation for 24 hours, makes strain quantity respectively reach 108A/mL, by each strain by described
The mass percent of strain mixes；
Mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium, composite bacteria agent medium pH 6.5, is inoculated with
Amount is 0.5%, cultivates 2 days at 30 DEG C, bacterium number in fermentation liquid is made to reach 3~5 × 108A/mL, obtains benefit-additives.
4. as described in claim 1 improve Tilapia mossambica immunity cultural method, which is characterized in that the 5th step it is daily
Management method specifically includes:
(1) Chinese medicine for improving immunity and nutriment mixed, fermented, the feed after mixing, fermentation is put into feed
In mixing chamber, indoor agitator arm is mixed by feed, feed is stirred；
(2) feed mixing chamber is put into water body, carries out driving movement to whole by the implementation fan of rear end, is fanned by the implementation of two sides
Maintain whole balance；
(3) water flow is drawn into inside filter device by pulsating pump and is filtered, by water it is intracorporal kill and devour, excrement stays in filtering
In box, the water after removal of impurities is discharged into water body again by filtering rear cover, carries out circulation removal of impurities to water body；
(4) feed is mixed indoor feed by seasoning spreading box to be sprinkled into water body, feeding is carried out to Tilapia mossambica, guarantees Tilapia mossambica energy
It enough eats with the fresh feed for improving immunity.
5. a kind of raising Tilapia mossambica immunity for realizing the cultural method described in claim 1 for improving Tilapia mossambica immunity
Cultivation apparatus, which is characterized in that it is described improve Tilapia mossambica immunity cultivation apparatus be provided with shell, opened among enclosure interior
Equipped with feed mixing chamber, housing rear end embedded with carrying out fan, housing forward end be it is coniform, control is offered inside housing forward end
Room has been screwed single-chip microcontroller, GPRS signal transmission device, GPS locator inside control room；
Shell lower end center weldment has connecting rod, and connecting rod lower end is welded with filter device, and bolt has filtering inside filter device
Trash separator, filtering and impurity removing device are connected to by pulsating pump with the water inlet pipe of filter device front end, filtering and impurity removing device be welded on filtering
The filter cartridge of device rear end is connected to；
Enclosure interior is fixed with battery on the right side of feed mixing chamber, battery and single-chip microcontroller with carry out fan driving motor,
GPRS signal transmission device, GPS locator, pulsating pump electrical connection.
6. improving the cultivation apparatus of Tilapia mossambica immunity as claimed in claim 5, which is characterized in that subordinate in feed mixing chamber
End is equipped with rotating electric machine by screw, and rotating electric machine upper end is welded with multiple agitator arms, rotating electric machine and battery, monolithic
Mechatronics, feed mixing chamber upper end offer feed opening, and feed opening is arranged with feeding cover, and sealing rubber is enclosed on the outside of feeding cover
Cushion rubber.
7. improving the cultivation apparatus of Tilapia mossambica immunity as claimed in claim 5, which is characterized in that housing rear end two sides are embedded in
There is steering fan, the steering fan position of two sides is symmetrical, and steering fan driving motor is electrically connected with single-chip microcontroller, battery.
8. improving the cultivation apparatus of Tilapia mossambica immunity as claimed in claim 5, which is characterized in that control room front end two sides are equal
Embedded with ultrasonic sensor, ultrasonic sensor is electrically connected with single-chip microcontroller, battery.
9. improving the cultivation apparatus of Tilapia mossambica immunity as claimed in claim 5, which is characterized in that shell lower end two sides are embedded in
There is seasoning spreading box, seasoning spreading box upper end offers discharge funnel, and discharge funnel upper end opening is connected to feed mixing chamber, under discharge funnel
Discharge roller, the discharge motor axis connection of discharge roller and side, discharge motor and single-chip microcontroller, battery are embedded at end opening
Electrical connection.
10. improving the cultivation apparatus of Tilapia mossambica immunity as claimed in claim 5, which is characterized in that filter cartridge is cylinder
Closed container, filter cartridge rear side have been bolted filtering rear cover, and filtering rear cover filter opening diameter is less than the diameter of sundries, filtering
Rear cover front end is filled with sponge, is filled with active carbon particle in filter cartridge.
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