CN110257308A - A kind of cultural method of organic selenium-rich nostoc - Google Patents
A kind of cultural method of organic selenium-rich nostoc Download PDFInfo
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Abstract
The present invention relates to a kind of cultural methods of organic selenium-rich nostoc, including Cordyceps militaris spawn 1) is trained selenium-enriched cordceps militaris fructification using selenium-rich solid medium；2) enzymatic hydrolysis is starched using the bacterium that colloid mill wears into the selenium-enriched cordceps militaris fructification, obtains the selenium-rich stoste for being rich in seleno-amino acids；3) the nostoc domestication of logarithmic growth phase is trained to the nostoc frond for adapting to selenium-rich culturing using selenium-enriched liquid culture medium；4) Fiber differentiation adapts to the nostoc frond of selenium-rich culturing, obtains the nostoc of organic selenium-rich.The seleno-amino acids that the present invention is obtained using the vegetable active selenium that enzymatic hydrolysis and activated carbon adsorption processing are converted through multi-stage biological effectively improve the edible safety of selenium-rich nostoc as the selenium source of culture selenium-rich nostoc.The phase is under the effect of the co-induction of nutrition and light quality after incubation, enhance the photosynthetic capacity of nostoc, promote the synthesis of nostoc protein, improve nostoc to the utilization rates of seleno-amino acids in selenium-enriched liquid culture medium, to improve the Se content in nostoc and obtain the nostoc of organic selenium-rich.
Description
Technical field
The present invention relates to the culture technology field of nostoc, specially a kind of cultural method of organic selenium-rich nostoc.
Background technique
Nostoc is spherical nostoc (Nostoc sphaeoides K ü tzing) in Nostoc (Nostoc Vauch)
Be commonly called as, it belongs to Cyanophyta (Cyanophyta), Cyanophyceae (Cyanophyceae), beads Cutleriales (Nostocales), read
Zhu Zao section (Nostocaceae).Nostoc is also known as green bird's nest, precious, is the good merchantable brand of food and medicament dual-purpose.The total egg of its dry matter
Bai Hanliang is 28.38%~52%, total reducing sugar 24.19%.Containing 18 kinds of amino acid, including 8 kinds of amino acid needed by human；It is rich in
VC、VEAnd B family vitamin and the high edible algae of a variety of mineral elements, especially calcium content.Protein content in nostoc
Height, the rich amino acids of constitutive protein matter provide material base for selenium-rich culturing, and nostoc is that a kind of production plant is living
The splendid algae of property selenium.
Selenium is nutrient necessary to a kind of animal body, and selenium can not synthesize in human body, so to meet human body to selenium
Demand, it is necessary to daily Selenium Supplement.By World Health Organization's requirement, daily Minimum requirements amount is 50 microgram selenium in human body diet,
And nutritional supplementation is advisable in 50~250 microgram selenium.578.3~2017 intake of WST, the reference of Chinese residents diet nutrient are taken the photograph
Enter amount third portion: in microelement, for all ages and classes and physiological status, it is specified that recommendation daily selenium intake.Science is examined
It examines and shows that selenium can also have antagonism with other some heavy metal elements, so body both can be enhanced in edible selenium-enriched food
Matter achievees the purpose that prevent disease, can also prevent human body heavy metal element and be poisoned.
Selenium is divided into two kinds in the existing way of nature: inorganic selenium and vegetable active selenium.Inorganic selenium refers generally to sodium selenite
And sodium selenate, it is obtained from the byproduct of metal mineral reserves；Inorganic selenium has biggish toxicity, and is not easy to be absorbed, be not suitable for people and
Animal uses.Vegetable active selenium is that selenium is combined by bioconversion and amino acid, is existed generally in the form of selenomethionine.
Currently, the vegetable active selenium that gains public acceptance to be human and animal allow using selenium source.
The existing patent No.: CN201811410255 and the patent No.: the patent of CN201811414608 discloses selenium-rich Ge Xian
In rice culture technique, used selenium source is sodium selenite, on October 27th, 2017, international cancer research institution, the World Health Organization
The carcinogenic substance inventory edit of announcement refers to, and in 3 class carcinogenic substance inventories, nostoc is rich as algae for selenium and selenium compound
Collection ability is strong.It can be seen that the selenium of the selenium-rich nostoc of prior art culture should exist simultaneously selenium compound and vegetable active selenium.Cause
This, nostoc absorbs and unconverted when remaining in the intracorporal inorganic selenium of algae and can directly cause with edible selenium-rich nostoc selenium-supply
Safety.
In order to reduce security risk problem when the above edible selenium-rich nostoc selenium-supply, the present invention is converted with edible fungus
Selenomethionine is the selenium source for cultivating selenium-rich nostoc, and indirect addition inorganic selenium (sodium selenite or sodium selenate), greatly
Reduce the toxicity of selenium in nostoc.
Summary of the invention
The purpose of the present invention is to provide a kind of cultural method of organic selenium-rich nostoc, solve to mention in above-mentioned background technique
Inorganic selenium culture selenium-rich nostoc is directlyed adopt out and is existed to eat safety issue when selenium-rich nostoc selenium-supply.
To achieve the above object, the invention provides the following technical scheme:
A kind of cultural method of organic selenium-rich nostoc, the specific steps are as follows:
Cordyceps militaris spawn is trained selenium-enriched cordceps militaris fructification using selenium-rich solid medium by step 1)；
The selenium-rich solid medium includes the raw material of following components by weight percent: 20~25 parts of selenium-enriched protein grass, glucose 2~
4 parts, 1~2 part of brown sugar, 5~8 parts of water soluble starch, 1~2 part of compound amino acid, 1~2 part of chlorella powder, 1~2 part of earthworm powder,
10~20 parts of nostoc breakage frond, 20~30 parts of wheat, 0.001~0.005 part of salt, 10~20 parts of pp plastic pellet, soil
20~30 parts of leachate, 100~200 parts of water；
The condition of culture includes: dark training and optical culture, dark 18~20 DEG C of temperature of training, and the period 3~4 days, the light of optical culture
Dark than being 6h:18h, light intensity is 500~1000lux, 19~23 DEG C of temperature, is cultivated 50~60 days；
Step 2) enzymatic hydrolysis is starched using the bacterium that colloid mill wears into the selenium-enriched cordceps militaris fructification, is obtained and is rich in seleno amino
The selenium-rich stoste of acid；
The nostoc domestication of logarithmic growth phase is trained using selenium-enriched liquid culture medium and adapts to selenium-rich culturing by step 3)
Nostoc frond；
The selenium-enriched liquid culture medium of the domestication culture includes the raw material of following composition by weight: selenium-rich stoste 30~50
Part, 0.05~1 part of magnesium sulfate, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, ferrous sulfate 0.01~
0.025 part, 0.02~0.05 part of potassium chloride, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
The domestication condition of culture includes: illumination 10000~15000lux of light intensity, and 20~30 DEG C of temperature, ventilation culture is all
Phase 20~30 days；
Step 4) Fiber differentiation adapts to the nostoc frond of selenium-rich culturing, obtains the nostoc of organic selenium-rich；
Selenium-enriched liquid culture medium used in the Fiber differentiation includes the raw material of following components by weight percent: selenium-rich stoste 30~50
Part, 0.05~0.08 part of urea, 0.5~1 part of magnesium sulfate, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, sulphur
It is sour 0.01~0.025 part ferrous, 0.02~0.05 part of potassium chloride, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
The inducing culturing condition includes: that wavelength is 620~645nm monochromatic light, 5000~6000lux of intensity of illumination, temperature
20~25 DEG C of degree is ventilated cultivation cycle 30~40 days.
Above-mentioned cultural method step 1) specifically comprises the following steps:
1) the raw material selenium-rich solid medium including following components by weight percent: 20~25 parts of selenium-enriched protein grass, glucose 2 is prepared
~4 parts, 1~2 part of brown sugar, 5~8 parts of water soluble starch, 1~2 part of compound amino acid, 1~2 part of chlorella powder, earthworm powder 1~2
Part, 10~20 parts of nostoc breakage frond, 20~30 parts of wheat, 0.001~0.005 part of salt, 10~20 parts of pp plastic pellet, soil
20~30 parts of earth leachate, 100~200 parts of water.
2) sterilize selenium-rich solid medium: solid culture being based on 121 DEG C of sterilizing 30min and is cooled to room temperature.
3) inoculation and culture: Cordyceps militaris spawn is connected on selenium-rich solid medium, secretly trains 3~4 at 18~20 DEG C of temperature
It switchs in Light To Dark Ratio be 6h:18h, 500~1000lux of light intensity, and light is trained 50~60 days at 19~23 DEG C of temperature.
4) it harvests: harvesting the fruiting bodies of cordyceps militaris from 0.5~1cm or more of culture medium to get selenium-enriched cordceps militaris fructification.
Above-mentioned cultural method step 2) specifically comprises the following steps:
1) defibrination: using colloid mill by step 1) the selenium-enriched cordceps militaris fructification, bacterium is worn by solid-liquid ratio 15-30g/L
Slurry.
2) it digests: bacterium slurry being transferred in fermentor, the papain of 2000~5000U/g is added, adjusts pH=6~7,
Temperature is set as 55~65 DEG C, and air agitation hydrolyzes 3~6h.
3) enzyme deactivation: 100~121 DEG C are set by fermentation jar temperature, 15~30min is handled, by unreacted Papain
Enzyme-deactivating and obtain enzymolysis liquid.
4) it adsorbs: adding 10~20g/L of active carbon of 2~3mm of partial size in enzymolysis liquid, adsorb 3~5h of free selenium and decoloration.
5) be centrifuged: the enzymolysis liquid after absorption is centrifuged 10~15min through 6000~8000r/min, and supernatant is taken to be rich in
The selenium-rich stoste of seleno-amino acids.
Above-mentioned cultural method step 3) specifically comprises the following steps:
1) the domestication selenium-enriched liquid culture medium including following components by weight percent raw material: 30~50 parts of selenium-rich stoste, sulfuric acid is prepared
0.5~1 part of magnesium, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, 0.01~0.025 part of ferrous sulfate, chlorination
0.02~0.05 part of potassium, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water.
2) domestication culture: press 10~15g/L inoculum concentration, connect the nostoc frond of logarithmic growth phase, light intensity be 10000~
15000lux under conditions of 20~30 DEG C, ventilation domestication culture 20~30 days, obtains the nostoc frond for adapting to selenium-rich culturing.
Above-mentioned cultural method step 4) specifically comprises the following steps:
1) the induction selenium-enriched liquid culture medium including following composition by weight raw material: 30~50 parts of selenium-rich stoste, urea is prepared
0.05~0.08 part, 0.05~1 part of magnesium sulfate, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, ferrous sulfate
0.01~0.025 part, 0.02~0.05 part of potassium chloride, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water.
2) by the inoculum concentration of 10~15g/L, the nostoc frond of selenium-rich culturing photo-induction culture: will be adapted to described in step 3)
Be connected in selenium-rich induced medium, be placed in wavelength be 620~645nm monochromatic light under, 5000~6000lux of light intensity, temperature 20~
At 25 DEG C, ventilation culture 30~40 days.
3) harvest drying: harvesting nostoc is cleaned repeatedly with clear water, and drying is in 50 DEG C of baking oven to get organic selenium-rich
Nostoc dry product.
In above-mentioned cultural method step 1),
Preferably, selenium-rich solid medium includes the raw material of following composition by weight: selenium-enriched protein grass 24 parts, 3 parts of glucose,
1.5 parts of brown sugar, water soluble starch 6,1.8 parts of compound amino acid, 2 parts of chlorella powder, 2 parts of earthworm powder, nostoc breakage frond 20
Part, 20 parts of wheat, 0.0045 part of salt, 12 parts of pp material, 25 parts of soil extract, 200 parts of water.
Preferably, it is 19 DEG C that the condition of culture, which includes: dark training temperature, and the period is 4 days, and the Light To Dark Ratio of light training is 6h:
18h, light intensity 800lux, temperature are 20 DEG C, and cultivation cycle is 60 days.
Preferably, the partial size of the pp plastic pellet for improving selenium-rich solid medium gas permeability is 3mm；
Generally pp plastic pellet is available there is the material of improvement to replace culture medium gas permeability.
In above-mentioned cultural method step 2),
Preferably, the solid-liquid ratio of grinding bacterium slurry is 20g/L.
Preferably, the additive amount of papain used in the enzymatic hydrolysis is 3000U/g, and temperature is 60 DEG C, and when hydrolysis is a length of
5h。
Preferably, the enzyme-removal temperature is 105 DEG C, handling duration 20min.
Preferably, it is 2.5mm that activated carbon grain used in the absorption, which is partial size, additive amount 15g/L, and when absorption is a length of
4h。
In above-mentioned cultural method step 3),
Preferably, domestication culture selenium-enriched liquid culture medium used includes the raw material of following composition by weight: 45 parts of selenium-rich stoste,
0.8 part of magnesium sulfate, 0.15 part of calcium nitrate, 0.025 part of sodium glycero-phosphate, 0.025 part of ferrous sulfate, 0.035 part of potassium chloride, chlorination
0.03 part of sodium, 1500 parts of water.
Preferably, the nostoc sphere diameter 3mm or so, inoculum concentration 12g/L of connect logarithmic growth phase are cultivated in domestication.
Preferably, it is 12000lux that domestication condition of culture, which includes illumination light intensity, and temperature is 25 DEG C, and ventilation cultivation cycle is 25
It.
In cultural method step 4) described above,
Preferably, the fluid nutrient medium of selenium-rich used in Fiber differentiation includes the raw material of following composition by weight: selenium-rich stoste 48
Part, 0.8 part of urea, 0.08 part of magnesium sulfate, 0.15 part of calcium nitrate, 0.025 part of sodium glycero-phosphate, 0.02 part of ferrous sulfate, chlorination
0.03 part of potassium, 0.03 part of sodium chloride, 1800 parts of water；
Preferably, it is 645nm feux rouges that the light of Fiber differentiation, which is wavelength, and light intensity 6000lux, temperature is 22 DEG C, ventilation culture
Period is 40 days；
Generally increase the urea for promoting the synthesis of nostoc protein in selenium-enriched liquid culture medium, it can be with Ge Xian can be promoted
The raw material substitution of rice synthetic proteins matter.
The invention also includes above-mentioned solid mediums or aforesaid liquid culture medium on organic selenium-rich nostoc cultural method
Application.
Soil extraction can be prepared by art methods in the present invention.
Raw materials used can be purchased from market in the present invention and obtain.
Cordyceps militaris spawn used is the strain in Changde Yan Di, also known as Shen Nong, a legendary ruler Mu Yuan agricultural development Co., Ltd strain library in the present invention.
The nostoc frond of logarithmic growth phase used in the present invention is raw for Changde Yan Di, also known as Shen Nong, a legendary ruler Mu Yuan agricultural development Co., Ltd
The nostoc sphaeroides of production.Compared with prior art, the present invention have it is following the utility model has the advantages that
The seleno that the present invention is obtained using the vegetable active selenium that enzymatic hydrolysis and activated carbon adsorption processing are converted through multi-stage biological
Selenium source of the amino acid as culture selenium-rich nostoc reduces containing for the selenium that dissociates in the selenium-enriched liquid culture medium for cultivating nostoc
Amount is not only that culture selenium-rich nostoc provides safer selenium source, also provides nutrition abundant for nostoc growth.Pass through nutrition
With the co-induction of light quality, nostoc is improved to the utilization rate of seleno-amino acids in selenium-enriched liquid culture medium, is improved in nostoc
Se content and obtain the nostoc of organic selenium-rich, using the multi-stage conversion effect of biology and enzymolysis and extraction technology, can be used
The seleno-amino acids of nostoc synthetic proteins matter avoid directly using inorganic selenium, effectively improve the edible peace of selenium-rich nostoc
Quan Xing.
Advantage of the invention:
1, cultivating the selenium source of selenium-rich nostoc is azolla filliculoidas and the indirect selenium source that Cordyceps militaris converts twice；
2, the selenium source for cultivating selenium-rich nostoc is the seleno-amino acids of the enzymolysis and extraction from selenium-enriched cordceps militaris fructification；
3, the pp plastic pellet being added in selenium-enriched cordceps militaris culture medium, is effectively improved culture medium gas permeability, prevents between wheat
Stickiness, improve base in gas exchange, promote mycelia growth, improve fruiting bodies of cordyceps militaris per unit area yield；
4, urea and benefit are acted on the co-induction of 645nm feux rouges light quality, enhance the photosynthetic capacity of nostoc, improve Ge Xian
Rice to the utilization rates of seleno-amino acids in selenium-rich culturing base, promote nostoc synthetic proteins matter and improve Se content in nostoc and
Culture obtains selenium-rich nostoc.
Specific embodiment
Combined with specific embodiments below, technical scheme in the embodiment of the invention is clearly and completely described, shows
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, all other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1: the culture of organic selenium-rich nostoc
A kind of cultural method of organic selenium-rich nostoc, the specific steps are as follows:
Cordyceps militaris spawn is trained selenium-enriched cordceps militaris fructification using selenium-rich solid medium by step 1), specifically include as
Lower step:
1) the raw material selenium-rich solid medium including following components by weight percent: selenium-enriched protein grass 24 parts, 3 parts of glucose is prepared,
1.5 parts of brown sugar, 6 parts of water soluble starch, 1.8 parts of compound amino acid, 2 parts of chlorella powder, 2 parts of earthworm powder, nostoc breakage frond
20 parts, 20 parts of wheat, 0.0045 part of salt, 12 parts of pp plastic pellet, 25 parts of soil extract, 200 parts of water.
2) sterilize selenium-rich solid medium: solid culture being based on 121 DEG C of sterilizing 30min and is cooled to room temperature.
3) inoculation and culture: Cordyceps militaris spawn is connected on selenium-rich solid medium, is secretly trained 4 days, is switched at 19 DEG C of temperature
It is 6h:18h, light intensity 800lux in Light To Dark Ratio, light is trained 60 days at 20 DEG C of temperature.
4) it harvests: harvesting the fruiting bodies of cordyceps militaris from culture medium 0.5cm or more to get selenium-enriched cordceps militaris fructification.
Step 2): enzymatic hydrolysis is starched using the bacterium that colloid mill wears into the selenium-enriched cordceps militaris fructification, is obtained and is rich in seleno ammonia
The selenium-rich stoste of base acid, specifically comprises the following steps:
1) defibrination: using colloid mill by step 1) the selenium-enriched cordceps militaris fructification, bacterium is worn by solid-liquid ratio 15-30g/L
Slurry.
2) it digests: bacterium slurry being transferred in fermentor, the papain of 3000U/g is added, adjusts pH=6~7, temperature is set
It is 60 DEG C, air agitation hydrolyzes 5h.
3) enzyme deactivation: 105 DEG C of processing 20min are set by fermentation jar temperature, unreacted Papain enzyme-deactivating is obtained
Obtain enzymolysis liquid.
4) it adsorbs: adding the active carbon 15g/L of partial size 2.5mm in enzymolysis liquid, adsorb free selenium and decoloration 4.5h.
5) be centrifuged: the enzymolysis liquid after absorption is centrifuged 15min through 6000r/min, and supernatant is taken to obtain rich in seleno-amino acids
Selenium-rich stoste.
Step 3): nostoc domestication of the fluid nutrient medium by logarithmic growth phase is configured into adaptation selenium-rich using selenium-rich stoste
The nostoc frond of culture, specifically comprises the following steps:
1) the domestication selenium-enriched liquid culture medium including following components by weight percent raw material: 45 parts of selenium-rich stoste, magnesium sulfate 0.8 is prepared
Part, 0.15 part of calcium nitrate, 0.025 part of sodium glycero-phosphate, 0.025 part of ferrous sulfate, 0.035 part of potassium chloride, 0.03 part of sodium chloride,
1500 parts of water.
2) domestication culture: press 12g/L inoculum concentration, connect the nostoc frond of logarithmic growth phase, light intensity be 12000lux, 25
Under conditions of DEG C, ventilation domestication culture 25 days, the nostoc frond for adapting to selenium-rich culturing is obtained.
Step 4): Fiber differentiation adapts to the nostoc frond of selenium-rich culturing, obtains the nostoc of organic selenium-rich, specifically includes
Following steps:
1) the induction selenium-enriched liquid culture medium including following composition by weight raw material: 48 parts of selenium-rich stoste, urea 0.08 is prepared
Part, 0.08 part of magnesium sulfate, 0.15 part of calcium nitrate, 0.025 part of sodium glycero-phosphate, 0.02 part of ferrous sulfate, 0.03 part of potassium chloride, chlorine
Change 0.03 part of sodium, 1800 parts of water.
2) photo-induction culture: by the inoculum concentration of 15g/L, the nostoc frond that selenium-rich culturing is adapted to described in step 3) is connected to
In selenium-rich induced medium, being placed in wavelength is under 645nm monochromatic light, light intensity 6000lux, at 22 DEG C of temperature, ventilation culture 40 days.
3) harvest drying: harvesting nostoc is cleaned repeatedly with clear water, and drying is in 50 DEG C of baking oven to get organic selenium-rich
Nostoc dry product.Selenium-rich nostoc obtained by being cultivated by embodiment 1, through detecting, nostoc selenium-rich effect such as the following table 1:
Se content and Se form testing result in 1 selenium-rich nostoc of table
| Detection project | Testing result |
| SeCys2 | 20.2% |
| MeSeys | 0.0% |
| Se4+ | 6.7% |
| SeMet | 73.1% |
| Se6+ | 0.0% |
| SeEt seleno ethionine | 0.0% |
| Total selenium/(mg/kg) | 2.03 |
| The total selenium of supernatant/(mg/kg) | 1.61 |
| Recovery rate | 79.6% |
Observe table testing result, show in the selenium-rich nostoc cultivated of the present invention, total Se content up to 2.03mg/kg, with
Cys, Met of constitutive protein matter are combined in nostoc, and ratio only accounts for 6.7%, i.e., in nostoc up to the free selenium of 93.3%, 4 valence
Selenium mainly exists in the form of selenomethionine.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being understood that.
Claims (9)
1. a kind of cultural method of organic selenium-rich nostoc, which comprises the following steps:
Cordyceps militaris spawn is trained selenium-enriched cordceps militaris fructification using selenium-rich solid medium by step 1)；
The selenium-rich solid medium includes the raw material of following composition by weight: 20~25 parts of selenium-enriched protein grass, 2~4 parts of glucose,
1~2 part of brown sugar, 5~8 parts of water soluble starch, 1~2 part of compound amino acid, 1~2 part of chlorella powder, 1~2 part of earthworm powder, Ge Xian
Damaged 10~20 parts of the frond of rice, 20~30 parts of wheat, 0.001~0.005 part of salt, 10~20 parts of pp plastic pellet, soil leaches
20~30 parts of liquid, 100~200 parts of water；
The condition of culture includes: dark training and optical culture, dark 18~20 DEG C of temperature of training, and the period 3~4 days, the Light To Dark Ratio of optical culture
For 6h:18h, light intensity is 500~1000lux, 19~23 DEG C of temperature, is cultivated 50~60 days；
Step 2) enzymatic hydrolysis is starched using the bacterium that colloid mill wears into the selenium-enriched cordceps militaris fructification, is obtained rich in seleno-amino acids
Selenium-rich stoste；
The nostoc domestication of logarithmic growth phase is trained the Ge Xian for adapting to selenium-rich culturing using selenium-enriched liquid culture medium by step 3)
Rice frond；
The selenium-enriched liquid culture medium of the domestication culture includes the raw material of following composition by weight: 30~50 parts of selenium-rich stoste, sulphur
0.05~1 part of sour magnesium, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, 0.01~0.025 part of ferrous sulfate,
0.02~0.05 part of potassium chloride, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
The domestication condition of culture includes: illumination 10000~15000lux of light intensity, and 20~30 DEG C of temperature, cultivation cycle 20 of ventilating
~30 days；
Step 4) Fiber differentiation adapts to the nostoc frond of selenium-rich culturing, obtains the nostoc of organic selenium-rich；
Selenium-enriched liquid culture medium used in the Fiber differentiation includes the raw material of following composition by weight: 30~50 parts of selenium-rich stoste, urine
0.05~0.08 part of element, 0.5~1 part of magnesium sulfate, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, sulfuric acid are sub-
0.01~0.025 part of iron, 0.02~0.05 part of potassium chloride, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
The inducing culturing condition includes: that wavelength is 620~645nm monochromatic light, 5000~6000lux of intensity of illumination, temperature 20
It~25 DEG C, ventilates cultivation cycle 30~40 days.
2. a kind of cultural method of organic selenium-rich nostoc according to claim 1, which is characterized in that step 1) is specifically wrapped
Include following steps:
1) preparation includes the selenium-rich solid medium of the raw material of following composition by weight: 20~25 parts of selenium-enriched protein grass, glucose 2~
4 parts, 1~2 part of brown sugar, water soluble starch 5~8,1~2 part of compound amino acid, 1~2 part of chlorella powder, 1~2 part of earthworm powder, Pueraria lobota
Celestial 10~20 parts of rice breakage frond, 20~30 parts of wheat, 0.001~0.005 part of salt, improve the material 10~20 of gas permeability
Part, 20~30 parts of soil extract, 100~200 parts of water；
2) sterilize selenium-rich solid medium: solid culture being based on 121 DEG C of sterilizing 30min and is cooled to room temperature；
3) inoculation and culture: Cordyceps militaris spawn is connected on selenium-rich solid medium, is secretly trained at 18~20 DEG C of temperature 3~4 days, is turned
To be 6h:18h, 500~1000lux of light intensity in Light To Dark Ratio, light is trained 50~60 days at 19~23 DEG C of temperature；
4) it harvests: harvesting the fruiting bodies of cordyceps militaris from 0.5~1cm or more of culture medium to get selenium-enriched cordceps militaris fructification.
3. a kind of cultural method of organic selenium-rich nostoc according to claim 1, which is characterized in that step 2) is specifically wrapped
Include following steps:
1) defibrination: using colloid mill by step 1) the selenium-enriched cordceps militaris fructification, bacterium slurry is worn by solid-liquid ratio 15-30g/L；
2) it digests: bacterium slurry being transferred in fermentor, the papain of 2000~5000U/g is added, adjusts pH=6~7, temperature
55~65 DEG C are set as, air agitation hydrolyzes 3~6h；
3) enzyme deactivation: 100~121 DEG C are set by fermentation jar temperature, 15~30min is handled, unreacted papain is gone out
It lives and obtains enzymolysis liquid；
4) it adsorbs: adding 10~20g/L of active carbon of 2~3mm of partial size in enzymolysis liquid, adsorb 3~5h of free selenium and decoloration；
5) be centrifuged: the enzymolysis liquid after absorption is centrifuged 10~15min through 6000~8000r/min, and supernatant is taken to obtain rich in seleno
The selenium-rich stoste of amino acid.
4. a kind of cultural method of organic selenium-rich nostoc according to claim 1, which is characterized in that step 3) is specifically wrapped
Include following steps:
1) the domestication selenium-enriched liquid culture medium of the raw material including following composition by weight: 30~50 parts of selenium-rich stoste, magnesium sulfate is prepared
0.5~1 part, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, 0.01~0.025 part of ferrous sulfate, potassium chloride
0.02~0.05 part, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
2) domestication culture: press 10~15g/L inoculum concentration, connect the nostoc frond of logarithmic growth phase, light intensity be 10000~
15000lux under conditions of 20~30 DEG C, ventilation domestication culture 20~30 days, obtains the nostoc frond for adapting to selenium-rich culturing.
5. a kind of cultural method of organic selenium-rich nostoc according to claim 1, which is characterized in that step 4) is specifically wrapped
Include following steps:
1) the induction selenium-enriched liquid culture medium of the raw material including following composition by weight: 30~50 parts of selenium-rich stoste, urea is prepared
0.05~0.08 part, 0.05~1 part of magnesium sulfate, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, ferrous sulfate
0.01~0.025 part,
0.02~0.05 part of potassium chloride, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
2) photo-induction culture: by the inoculum concentration of 10~15g/L, the nostoc frond that selenium-rich culturing is adapted to described in step 3) is connected to
In selenium-rich induced medium, being placed in wavelength is 20~25 DEG C of 5000~6000lux of light intensity, temperature under 620~645nm monochromatic light
Under, ventilation culture 30~40 days；
3) harvest drying: harvesting nostoc is cleaned repeatedly with clear water, and drying is in 50 DEG C of baking oven to get organic selenium-rich Ge Xian
Meter Gan Pin.
6. a kind of cultural method of organic selenium-rich nostoc according to claim 1 or 2, which is characterized in that the culture
The selenium-rich solid medium of selenium-enriched cordceps militaris, the raw material including following composition by weight: 20~25 parts of selenium-enriched protein grass, glucose 2
~4 parts, 1~2 part of brown sugar, water soluble starch 5~8,1~2 part of compound amino acid, 1~2 part of chlorella powder, 1~2 part of earthworm powder,
10~20 parts of nostoc breakage frond, 20~30 parts of wheat, 0.001~0.005 part of salt, 10~20 parts of pp plastic pellet, soil
20~30 parts of leachate, 100~200 parts of water；
Preferably, the selenium-rich solid medium, the raw material including following composition by weight: selenium-enriched protein grass 24 parts, glucose 3
Part, 1.5 parts of brown sugar, water soluble starch 6,1.8 parts of compound amino acid, 2 parts of chlorella powder, 2 parts of earthworm powder, nostoc breakage frond
20 parts, 20 parts of wheat, 0.0045 part of salt, 12 parts of pp plastic pellet, 25 parts of soil extract, 200 parts of water；
The condition of culture includes: dark training and optical culture, dark 18~20 DEG C of temperature of training, and the period 3~4 days, the Light To Dark Ratio of optical culture
For 6h:18h, light intensity is 500~1000lux, 19~23 DEG C of temperature, is cultivated 50~60 days；
Preferably, the condition of culture includes: dark training and optical culture, dark 19 DEG C of temperature of training, and the period 4 days, the Light To Dark Ratio of optical culture
For 6h:18h, light intensity 800lux, 20 DEG C of temperature, cultivate 60 days.
7. a kind of cultural method of organic selenium-rich nostoc according to claim 1 or 4, which is characterized in that the domestication
Culture selenium-enriched liquid culture medium includes the raw material of following composition by weight: 30~50 parts of selenium-rich stoste, 0.5~1 part of magnesium sulfate, nitre
0.1~0.2 part of sour calcium, 0.01~0.03 part of sodium glycero-phosphate, 0.01~0.025 part of ferrous sulfate, potassium chloride 0.02~0.05
Part, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
Preferably, the raw material including following composition by weight: domestication selenium-enriched liquid culture medium includes the raw material of following weight group component:
45 parts of selenium-rich stoste, 0.8 part of magnesium sulfate, 0.15 part of calcium nitrate, 0.025 part of sodium glycero-phosphate, 0.025 part of ferrous sulfate, chlorination
0.035 part of potassium, 0.03 part of sodium chloride, 1500 parts of water；
Domestication condition of culture include: by 10~15g/L inoculum concentration connect nostoc domestication culture, light intensity be 10000~
15000lux, under conditions of 20~30 DEG C, ventilation domestication culture 20~30 days；
Preferably, domestication condition of culture includes: inoculum concentration 12g/L, and the nostoc sphere diameter of logarithmic growth phase is 3mm or so, light intensity
For 12000lux, 25 DEG C of temperature, ventilation domestication cultivation cycle is 25 days.
8. a kind of cultural method of organic selenium-rich nostoc according to claim 1 or 5, which is characterized in that the induction
Selenium-enriched liquid culture medium includes the raw material of following parts by weight: 30~50 parts of selenium-rich stoste, 0.05~0.08 part of urea, magnesium sulfate
0.05~1 part, 0.1~0.2 part of calcium nitrate, 0.01~0.03 part of sodium glycero-phosphate, 0.01~0.025 part of ferrous sulfate, chlorination
0.02~0.05 part of potassium, 0.02~0.05 part of sodium chloride, 1000~2000 parts of water；
Preferably, the raw material including following composition by weight: 48 parts of selenium-rich stoste, 0.08 part of urea, 0.8 part of magnesium sulfate, calcium nitrate
0.15 part, 0.025 part of sodium glycero-phosphate, 0.02 part of ferrous sulfate, 0.03 part of potassium chloride, 0.03 part of sodium chloride, 1800 parts of water；
The photo-induction condition of culture includes: the inoculum concentration by 10~15g/L, meets the nostoc frond for adapting to selenium-rich culturing, Yu Bo
Under a length of 620~645nm monochromatic light, 5000~6000lux of light intensity, at 20~25 DEG C of temperature, ventilate Fiber differentiation 30~40 days.
Preferably, photoinduction condition of culture includes: inoculum concentration 15g/L, the feux rouges of a length of 645nm of light wave, light intensity 6000lux,
It 20 DEG C of temperature, ventilates cultivation cycle 40 days.
9. selenium-enriched liquid culture medium described in selenium-rich solid medium or claim 7 described in claim 6 is in organic selenium-rich Ge Xian
Application in rice culture.
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