CN110251266B

CN110251266B - Rat noise and carbon monoxide integrated combined exposure contamination experimental device

Info

Publication number: CN110251266B
Application number: CN201910555153.5A
Authority: CN
Inventors: 刘畅; 曹佳; 孙磊; 敖琳; 周妮娅; 陈卿
Original assignee: Army Medical University
Current assignee: Army Medical University
Priority date: 2019-06-25
Filing date: 2019-06-25
Publication date: 2021-06-04
Anticipated expiration: 2039-06-25
Also published as: CN110251266A

Abstract

To the defect that current device can't satisfy carbon monoxide and noise and expose the contamination simultaneously, this application provides a rat noise and carbon monoxide integration joint exposure contamination experimental apparatus. The noise generation chamber provided with the Bluetooth sound box and the gas mixing chamber are assembled and then embedded into the combined exposure cabin, a rat is placed in the mouth-nose contamination cup through a door body, and carbon monoxide is introduced into the gas mixing chamber through an air inlet; noise enters the joint exposure cabin through the sound transmission hole; because the oronasal toxicant cup is communicated with the combined exposure cabin, rats in the oronasal toxicant cup can be simultaneously subjected to the combined exposure of carbon monoxide and noise. The device can meet the experimental requirement of carbon monoxide and noise exposure at the same time. In addition, the mouth-nose contamination cup can limit the movement of the rat, so that the real situation that the movement of cabin operating personnel is limited can be simulated, and the distances between the ear-nose of the rat and a noise source and a gas pollution source are basically consistent, thereby improving the scientificity of an experimental result.

Description

Rat noise and carbon monoxide integrated combined exposure contamination experimental device

Technical Field

The invention relates to the field of experimental devices, in particular to a rat noise and carbon monoxide integrated exposure contamination experimental device.

Background

With the deep progress of the economic, urban and industrial processes, the life style and living environment of human beings are also greatly changed, and a large number of environmental harmful factors such as carbon monoxide and noise generated in industrial production, transportation and urban construction seriously affect the health of human beings. According to statistics, the average traffic noise intensity of 40% of residents in European Union countries per day is about 55dB, and the average traffic noise intensity of some developing countries even reaches 75-80 dB; the average traffic noise in most cities in China is mostly 76-81 dB, and the industrial noise intensity is mostly 85-95 dB. Carbon monoxide comes from industrial and mining enterprises, transportation, heating boilers, fireworks and crackers, and the like, wherein the emission of automobile waste gas is the most main source of carbon monoxide. In addition, the concentration of carbon monoxide in local areas is also increased by disastrous events such as fires, pit explosions, and the like. In some working environments, such as military working environments of heavy diesel locomotives, heavy trucks, tanks, armored cars and the like, the pollution condition of carbon monoxide and noise is more prominent, and the physical and psychological health of drivers and passengers is seriously threatened.

The world health organization indicates that noise can not only cause damage to the auditory system such as noise deafness, but also cause various health problems in the body such as cardiovascular diseases, metabolic diseases, sleep disorders, and the like. Carbon monoxide causes the pathological changes of multiple organs mainly caused by the functional impairment of the central nervous system, especially the serious impairment of the cardiovascular system, and the sequelae with larger harm are delayed encephalopathy, and the symptoms have higher disability rate. Carbon monoxide poisoning is investigated as the most common cause of accidental death in many developed and developing countries, where the mortality rate of acute poisoning carbon monoxide poisoning may exceed 50%. At present, the health damage effect and mechanism of single factors such as carbon monoxide or noise to occupational personnel are relatively clear, but when two harmful factors act on the human body simultaneously, the damage effect and the specific mechanism are not clear. And the combined pollution of carbon monoxide and noise in some special professional workplaces is very common, and the combined exposure of high-concentration/strength carbon monoxide and noise in military operation environments such as heavy diesel locomotives, heavy trucks, tanks, armored cars and the like is very serious. Therefore, the research on the carbon monoxide and noise combined exposure contamination and mechanism is carried out aiming at the special operation places, and the method has important significance for effectively preventing occupational harmful factor damage and enhancing operation efficiency. The construction of the carbon monoxide and noise combined exposure contamination experimental device is an important basis and precondition for developing research on damage effects, mechanisms and protective measures.

Through the research on the prior art, the applicant finds that carbon monoxide and noise belong to two harmful factors with different physical properties, and an integrated combined exposure contamination device is difficult to design, so that no relevant literature report is found at present. The existing experimental equipment can only realize the exposure and contamination of carbon monoxide or noise alone, and cannot be directly used for the combined exposure simulation contamination experiment of two factors.

Disclosure of Invention

The invention provides a rat noise and carbon monoxide integrated combined exposure contamination experimental device aiming at the defect that the existing device can not meet the requirement of simultaneous exposure contamination of carbon monoxide and noise.

The application provides a rat noise and carbon monoxide integration joint exposure contamination experimental apparatus, includes: the device comprises a gas mixing chamber, a noise generation chamber, a combined exposure cabin and muzzle toxicant exposure cups, wherein the gas mixing chamber is arranged at the upper part of the combined exposure cabin, and the muzzle toxicant exposure cups are distributed at the periphery of the combined exposure cabin;

the gas mixing chamber is provided with a gas inlet and a gas outlet, the bottom of the gas mixing chamber is detachably provided with a noise generation chamber, the bottom of the noise generation chamber is provided with a sound transmission hole, the gas mixing chamber and the noise generation chamber are respectively communicated with the combined exposure cabin through the gas outlet and the sound transmission hole, and the combined exposure cabin is provided with a gas outlet;

one end of the oronasal contamination cup is provided with a detachable door body, the other end of the oronasal contamination cup is provided with an opening, and the oronasal contamination cup is communicated with the combined exposure cabin through the opening.

Preferably, the air inlet includes carbon monoxide import and air intlet, the carbon monoxide import with air intlet is located respectively the roof and the lateral wall of gas mixing room, the gas outlet is located the bottom of gas mixing room.

Preferably, the gas mixing chamber further comprises a mixing fan for stirring the mixed gas, and the mixing fan is arranged inside the gas mixing chamber and is positioned between the gas inlet and the gas outlet.

Preferably, the side wall of the combined exposure cabin is provided with a through hole, and the oronasal contamination cup penetrates through the through hole to be communicated with the combined exposure cabin in a sealing way; the mouth and nose contamination cup comprises a cylindrical cup body, a cylindrical cavity and a conical cavity, wherein the cylindrical cup body, the cylindrical cavity and the conical cavity are sequentially connected with the door body, the door body is positioned on the outer side of the through hole, the conical cavity is positioned on the inner side of the through hole, and the cylindrical cavity is connected with the cylindrical cup body in a nested manner.

Preferably, the opening is a strip-shaped opening arranged on the conical cavity, and the size of the strip-shaped opening is adapted to the ear of a rat.

Preferably, the bottom of the oronasal contamination cup is detachably connected with a feces storage groove.

Preferably, the cylindrical cup body is internally provided with an excrement storage groove and a strip-shaped channel which extends along the axial direction of the excrement storage groove.

Preferably, the side wall of the joint exposure cabin is also provided with a monitoring port.

Preferably, the device further comprises a rubber plug matched with the exhaust port.

Preferably, the combined exposure cabin is a cylinder, the gas mixing chamber and the noise generation chamber are both positioned at the center of the combined exposure cabin, and the oronasal contamination cups are uniformly distributed on the periphery of the combined exposure cabin.

When the device is used, the noise generation chamber provided with the Bluetooth sound box and the gas mixing chamber are assembled and then are placed in the combined exposure cabin, a rat is placed in the oronasal contamination cup through the door body, carbon monoxide gas is introduced through the gas inlet of the gas mixing chamber, enters the combined exposure cabin through the gas outlet and is discharged through the gas outlet; the sound played by the Bluetooth sound box enters the joint exposure cabin through the sound transmission hole; because the mouth-nose contamination cup is communicated with the joint exposure cabin, rats in the mouth-nose contamination cup can be simultaneously subjected to joint exposure of carbon monoxide gas and noise. The device can meet the experimental requirement of carbon monoxide and noise exposure at the same time. In addition, the device can limit the moving range of the rat through the mouth-nose contamination cup, so that the distance between the rat and a noise source and the distance between the rat and a gas pollution source are basically consistent, and the controllability and the accuracy of an experimental result are improved.

Drawings

For a clearer explanation of the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a rat noise and carbon monoxide integrated exposure contamination experiment apparatus according to an embodiment of the present invention;

fig. 2 is a top view of a rat noise and carbon monoxide integrated exposure contamination experimental apparatus provided in an embodiment of the present invention;

FIG. 3 is a bottom view of a rat noise and CO integrated exposure contamination experimental apparatus according to an embodiment of the present invention;

FIG. 4 is a front view of an experimental apparatus for rat noise and carbon monoxide integrated exposure contamination provided in an embodiment of the present invention;

FIG. 5 is an exploded view of a rat noise and CO integrated exposure contamination experimental apparatus according to an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a combined exposure cabin provided by an embodiment of the invention;

FIG. 7 is a schematic structural diagram of a noise generation chamber according to an embodiment of the present invention;

FIG. 8 is a schematic structural view of a gas mixing chamber according to an embodiment of the present invention;

FIG. 9 is a schematic, exploded view of a noise generation chamber according to an embodiment of the present invention;

FIG. 10 is a schematic structural view of a oronasal toxicant cup according to an embodiment of the invention;

FIG. 11 is a schematic exploded view of a oronasal toxicant cup according to an embodiment of the invention;

fig. 12 is a schematic structural view of a rubber stopper according to an embodiment of the present invention;

shown in the figure:

the device comprises a gas mixing chamber-1, a noise generation chamber-2, a combined exposure cabin-3, a telescopic oronasal toxicant exposure cup-4, an air inlet-5, a carbon monoxide inlet-51, an air inlet-52, an opening-6, a mixing fan-7, an air outlet-8, a sound transmission hole-9, a monitoring hole-10, a through hole-11, an air outlet-12, a conical cavity-13, a strip-shaped window-14, a cylindrical cavity-15, a cylindrical cup body-16, a door body-17, a strip-shaped channel-18, an excrement storage groove-19 and a rubber plug-20.

Detailed Description

In order to make those skilled in the art better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making creative efforts based on the embodiments of the present invention, shall fall within the protection scope of the present invention.

Aiming at the defect that the existing device can not meet the requirement of simultaneous exposure of carbon monoxide and noise, the rat noise and carbon monoxide integrated exposure contamination experimental device is provided.

Referring to fig. 1 to 5, a schematic structural diagram, a top view, a bottom view, a front view and an exploded view of a rat noise and carbon monoxide integrated combined exposure contamination experimental apparatus according to an embodiment of the present invention are respectively shown. As can be seen from fig. 1-5, the apparatus comprises: the device comprises a gas mixing chamber 1, a noise generating chamber 2, a combined exposure cabin 3 and an oronasal contamination cup 4, wherein the gas mixing chamber 1, the noise generating chamber 2 and the oronasal contamination cup 4 are communicated with the combined exposure cabin 3, and the gas mixing chamber 1 and the noise generating chamber 2 respectively provide a carbon monoxide gas source and a noise source for a rat placed in the oronasal contamination cup 4, so that the rat can be simultaneously subjected to combined exposure of carbon monoxide gas and noise.

Wherein, the gas mixing chamber 1 is arranged at the upper part of the combined exposure cabin 3, the noise generation chamber 2 is detachably connected at the bottom of the gas mixing chamber 1, and the nose and mouth contamination cups 4 are distributed at the periphery of the combined exposure cabin 3. Referring to fig. 6, a schematic structural diagram of a combined exposure cabin according to an embodiment of the present invention is shown. As can be seen from fig. 6, in the present embodiment, the upper surface and the side surface of the joint exposure chamber 3 are both provided with through holes 11, and the shape and the size of the through holes 11 are respectively matched with the gas mixing chamber 1 and the oronasal contamination cup 4. When the device is used, the gas mixing chamber 1 and the oronasal toxicant exposure cup 4 are installed in the joint exposure cabin 3 through the through holes, and after the experiment is finished, the gas mixing chamber 1 and the oronasal toxicant exposure cup 4 can be detached from the through holes.

The gas mixing chamber 1 is also provided with a gas inlet 5 and a gas outlet 8, in the embodiment, the gas inlet 5 is positioned above the joint exposure cabin 3, so that the gas inlet 5 can be conveniently connected with each gas inlet pipeline; the air outlet 8 extends to the inside of the joint exposure chamber 3 so as to communicate with the joint exposure chamber 3.

Referring to fig. 7, a schematic structural diagram of a noise generation chamber according to an embodiment of the invention is shown. As can be seen from fig. 7, the bluetooth speaker can be placed inside the noise generation chamber 2, and parameters such as the intensity, frequency, duration, etc. of the noise played by the bluetooth speaker can be adjusted through the external device. During the experiment, a noise dosimeter (not shown in the figure) is arranged below the mouth and nose contamination cup 4 on the inner wall of the joint exposure cabin 3 to monitor the noise intensity and the like in real time, so that the noise related parameters can be conveniently adjusted. The bottom of the noise generating chamber 2 is provided with a plurality of sound transmission holes 9, and the noise generating chamber 2 can be communicated with the joint exposure cabin 3 through the sound transmission holes 9. In this embodiment, the noise generation chamber 2 can be connected with the gas mixing chamber 1 in a nested manner, so that the noise generation chamber is stable and is not easy to drop.

One end of the oronasal contamination cup 4 is provided with a detachable door body 17, the door body 17 is used for placing or taking out a rat used for an experiment, and the door body 17 is always kept in a closed state in the experiment process so as to prevent carbon monoxide in the joint exposure cabin 3 from escaping. In this embodiment, the other end of the oronasal contamination cup 4 is provided with an opening 6, and the oronasal contamination cup 4 can be communicated with the combined exposure cabin 3 through the opening 6.

In addition, in order to keep the concentration of carbon monoxide in the experimental process, the combined exposure cabin 3 is also provided with a plurality of exhaust ports 12, carbon monoxide enters from the air inlet 5, and is exhausted from the exhaust ports 12 along with air, carbon dioxide and other gases after the respiration of a rat, so that the gases in the combined exposure cabin 3 can be in a dynamic balance state, the concentration of the carbon monoxide in the combined exposure cabin 3 is ensured to be kept in a stable state, and the reasonability and the accuracy of the experiment are enhanced.

When the device is used, the noise generation chamber 2 provided with the Bluetooth sound box and the gas mixing chamber 1 are assembled and then are placed into the combined exposure cabin 3, a rat is placed into the mouth and nose contamination cup 4 through the door body 17, carbon monoxide gas is introduced through the gas inlet 5 of the gas mixing chamber 1, enters the combined exposure cabin 3 through the gas outlet 8 and is discharged through the gas outlet 12; the sound played by the Bluetooth sound box enters the joint exposure cabin 3 through the sound transmission hole 9; since the oronasal toxicant exposure cup 4 is communicated with the joint exposure cabin 3, rats in the oronasal toxicant exposure cup 4 can be simultaneously subjected to the joint action of carbon monoxide gas and noise. The device can meet the experimental requirement of carbon monoxide and noise exposure at the same time.

In addition, the existing experimental device for simulating carbon monoxide contamination generally arranges rats in a contamination cabin, the rats can concentrate on the corners with small concentration, and the movement of the rats can cause the uneven concentration distribution of the carbon monoxide in the contamination cabin, thereby causing large experimental error; similar problems exist in the conventional rat noise exposure device, and the rat is piled up at a corner to cause large difference of noise intensity contacted by different rats. Only place a rat in every oronasal contamination cup 4 in this device, can prevent effectively that the rat from pricking the heap in the cabin, through the length of adjusting oronasal contamination cup 4, restrict the home range of rat, can make the rat and the distance of noise source, gas pollution source unanimous basically simultaneously to improve the controllability and the accuracy of experimental result.

To further improve the homogeneity of the carbon monoxide gas in the combined exposure chamber 3, the preferred embodiment of the present application pre-mixes the carbon monoxide gas in the gas blending chamber 1. Specifically, please refer to fig. 8 and fig. 9, which are a schematic structural diagram of a gas mixing chamber according to an embodiment of the present invention and a schematic disassembly diagram of a noise generation chamber according to an embodiment of the present invention. As shown in fig. 8 and 9, the air inlet 5 includes a carbon monoxide inlet 51 and an air inlet 52, the carbon monoxide inlet 51 and the air inlet 52 are respectively located on the top wall and the side wall of the gas blending chamber 1, and the air outlet 8 is located at the bottom of the gas blending chamber 1. The carbon monoxide inlet 51 and the air inlet 52 may be provided in other shapes such as a circle, a rectangle, etc.

Preferably, the gas mixing chamber 1 may be provided with a carbon monoxide inlet 51 on the top wall and two air inlets 52 symmetrically on the side wall. The air inlets 52 are arranged to be two, so that the concentration of the carbon monoxide can be quickly and uniformly diluted and adjusted, and the accuracy of the experiment can be improved. Gas mixing chamber 1 still includes mixing fan 7 that is used for stirring mist, mixing fan 7 sets up the inside of gas mixing chamber 1, and is located the air inlet 5 with between the gas outlet 8.

The principle of carbon monoxide gas mixing is as follows: air and carbon monoxide gas are introduced through mutually vertical gas inlets, and the gas impacts with each other to realize the primary mixing of the mixed gas; realize the mixing again of mist under the wind-force stirring effect of mixing fan 7, in addition, under the effect of mixing fan 7, the final mixing of mist is realized to the striking effect of mist and 1 roof of gas mixing room, guarantees to get into the concentration of jointly exposing every local carbon monoxide in the cabin 3 unanimously, improves the homogeneity that carbon monoxide infects the poison in the rat experiment.

During the experiment, carbon monoxide gas is rapidly accumulated and diffused in the gas blending chamber 1 through the carbon monoxide inlet 51; high-pressure pure dry air is introduced into the gas mixing chamber 1 through the air inlet 52, carbon monoxide and air are fully mixed by adjusting the flow rate of the air and the rotating speed of the mixing fan 7, and the air enters the combined exposure cabin 3 through the air outlet 8, so that carbon monoxide inhalation contamination is carried out on rats. Meanwhile, the toxicity evaluation and other experiments can be carried out on carbon monoxide with different concentrations and noise exposure with different intensities by adjusting the volume of the Bluetooth sound box and setting the noise exposure intensity; after the experiment is finished, air is rapidly discharged into the gas blending chamber 1 and the combined exposure cabin 3, so that carbon monoxide gas is rapidly discharged through the exhaust port 12 of the combined exposure cabin 3.

In another embodiment, the rat may also introduce air into the carbon monoxide inlet 51 and two different harmful gases into the two air inlets 52, so as to establish a rat model of combined exposure of two harmful gases and noise.

Referring to fig. 10, a schematic structural view of a oronasal contamination cup according to an embodiment of the present invention is shown. As shown in figure 10, the oronasal contamination cup 4 comprises a cylindrical cup body 16, a cylindrical cavity 15 and a conical cavity 13 which are sequentially connected with a door body 17, wherein the door body 17 is positioned at the outer side of the through hole 11, and the conical cavity 13 is positioned at the inner side of the through hole 11. In the preferred embodiment, the cylindrical cavity 15 is nested with the cylindrical cup 16, the cylindrical cavity 15 can move telescopically in the cylindrical cup 16, the moving range of the rat can be further limited, and the exposing doses of carbon monoxide and noise of the rat can be basically consistent by adjusting the length of the oronasal contamination cup 4 and the position of the oronasal contamination cup in the combined exposing cabin 3.

In order to further enhance the uniformity of the exposure dosage of carbon monoxide gas and noise, the combined exposure cabin 3 is a cylinder, the gas mixing chamber 1 and the noise generation chamber 2 are both positioned at the central position of the combined exposure cabin 3, and the nose and mouth toxicant exposure cups 4 are uniformly distributed on the periphery of the combined exposure cabin 3.

In this embodiment, the conical cavity 13 may be a conical cavity tapering towards the interior of the joint exposure chamber 3, and the size of the conical cavity 13 is adapted to the shape of the mouth and nose of the rat. By the arrangement, the rat can be infected by inhaling through the mouth and the nose, so that secondary damage caused by licking hair after the rat is infected by virus is effectively avoided; and can make rat continuously and uniformly inhale carbon monoxide to contaminate. Of course, the conical cavity 13, the cylindrical cavity 15 and the lower surface of the cylindrical cup 16 can also be designed to be flat to improve the stability of the rat in the oronasal toxicant cup 4.

Fig. 11 is a schematic illustration showing a split view of a oronasal contamination cup according to an embodiment of the present invention. As can be seen from fig. 11, in the present embodiment, the opening 6 of the oronasal contamination cup 4 communicated with the joint exposure cabin 3 is a strip-shaped opening 14 arranged on the conical cavity 13, and the size of the strip-shaped opening 14 is adapted to the ear of the rat. The arrangement of the strip-shaped opening 14 can enable the heat of the body of a rat to be discharged in time, and prevent the animal from being damaged due to unsmooth discharge of the body heat in the animal poisoning process caused by the undersize space of the mouth-nose poisoning cup 4. Meanwhile, the strip-shaped opening 14 can avoid shielding animal ears, and the accuracy and the scientificity of the experiment are further improved. In this embodiment, the door 17 is a sealing cover adapted to the cylindrical cup 16, so that it is convenient to take and place animals, and it is ensured that carbon monoxide in the combined exposure chamber 3 cannot escape from the nose and mouth contamination cup 4.

When the number of the arranged oronasal toxicant exposure cups 4 is more than the number required in the experiment, the idle oronasal toxicant exposure cups 4 can be placed on the side wall of the combined exposure cabin 3, the combined exposure cabin 3 can be sealed without additionally designing a conical plug, carbon monoxide is prevented from escaping from the through hole 11 on the side wall of the combined exposure cabin 3, and the stability of the concentration of the carbon monoxide in the combined exposure cabin 3 is ensured.

In order to collect and clean animal excrement, the bottom of the oronasal toxicant exposure cup 4 is detachably connected with an excrement storage groove 19. In this embodiment, the cylindrical cup 16 has a feces storage groove 19 and a strip passage 18 extending axially along the feces storage groove 19. When an animal is tested, the excrement and urine of the animal can be discharged into the excrement storage groove 19 through the strip-shaped channel 18, and the excrement storage groove 19 can be drawn out after the test is finished, so that the insides of the oronasal contamination cup 4 and the combined exposure cabin 3 are kept clean; and the excrement storage groove 9 is simple to take and place, convenient to wash and time-saving and labor-saving.

In other embodiments of the present application, the feces storage groove 19 may be provided outside the oronasal toxicant cup 4. The bottom of the mouth-nose poison cup 4 is provided with a slot, and the excrement storage groove 19 is connected with the outside of the mouth-nose poison cup 4 in a clamping manner. The oronasal contamination cup 4 is usually a cylinder structure matched with the through hole 11, excrement produced by animals can slide to the bottom of the oronasal contamination cup 4 through the side wall with radian of the oronasal contamination cup 4 and enter the excrement storage groove 19 through the bottom slot, and only the excrement storage groove 19 and the oronasal contamination cup 4 need to be separated when the excrement is cleaned.

Referring to fig. 1, 2, 4 and 6, in the embodiment of the present application, a monitoring port 10 may be further disposed on a side wall of the combined exposure cabin 3, so as to conveniently connect a temperature monitoring sensor, a humidity monitoring sensor, a carbon monoxide monitoring sensor, and the like to the monitoring port 10, and monitor and control the temperature, humidity and concentration of carbon monoxide at different positions. The monitor port 10 may have a circular shape or a rectangular shape, and the like, and it is preferable that the monitor port 10 has a circular shape and that a plurality of monitor ports 10 are provided outside the joint exposure chamber 3.

Further, a matched rubber plug may be disposed at the position of the monitoring port 10, and other devices such as a sealing door may also be disposed. The simple structure of rubber buffer, convenient processing is used, consequently, preferentially chooses for use and sets up the rubber buffer at the passway mouth of monitoring mouth 10 to prevent that carbon monoxide gas from overflowing from monitoring mouth 10, guarantee to jointly expose the stability of carbon monoxide in cabin 3 (can be preferred set up to monitoring the passageway, inside opening sets up directly over the animal head).

The bottom of the joint exposure cabin 3 is provided with an exhaust port 12, a rubber plug can be arranged at the position of the exhaust port 12, and other devices such as a sealing door can also be arranged, wherein the rubber plug is preferably arranged at the position of the exhaust port 12. Fig. 12 is a schematic structural view of a rubber stopper according to an embodiment of the present invention. In the experimental process, if the concentration of the carbon monoxide gas in the joint exposure cabin 3 can not reach the preset concentration all the time, the plurality of exhaust ports 12 can be properly plugged in a mode of capping rubber plugs, so that the concentration of the carbon monoxide gas in the joint exposure cabin 3 can quickly reach the preset concentration.

The gas mixing chamber 1, the noise generation chamber 2, the combined exposure cabin 3 and the telescopic oronasal contamination cup 4 are all made of transparent materials, so that an experimenter can observe the contamination condition of the animal in the oronasal contamination cup 4 in the experiment process.

Of course, the above description is not limited to the above examples, and the undescribed technical features of the present invention can be implemented by or using the prior art, and will not be described herein again; the above embodiments are merely for illustrating the technical solutions of the present invention and not for limiting the present invention, and the present invention has been described in detail with reference to the preferred embodiments, and those skilled in the art should understand that changes, modifications, additions or substitutions which are made by those skilled in the art within the spirit of the present invention are also within the scope of the claims of the present invention.

Claims

1. The utility model provides a rat noise and carbon monoxide integration joint exposure contamination experimental apparatus which characterized in that includes: a gas mixing chamber (1), a noise generating chamber (2), a combined exposure cabin (3) and a mouth and nose contamination cup (4), wherein,

the gas mixing chamber (1) is arranged at the upper part of the combined exposure cabin (3), and the mouth and nose toxicant exposure cups (4) are distributed at the periphery of the combined exposure cabin (3);

the air mixing chamber (1) is provided with an air inlet (5) and an air outlet (8), the bottom of the air mixing chamber (1) is detachably provided with a noise generation chamber (2), the bottom of the noise generation chamber (2) is provided with a sound transmission hole (9), the air mixing chamber (1) and the noise generation chamber (2) are communicated with the joint exposure cabin (3) through the air outlet (8) and the sound transmission hole (9) respectively, and the joint exposure cabin (3) is provided with an air outlet (12);

one end of the oronasal toxicant exposure cup (4) is provided with a detachable door body (17), the other end of the oronasal toxicant exposure cup is provided with an opening (6), and the oronasal toxicant exposure cup (4) is communicated with the combined exposure cabin (3) through the opening (6);

the gas inlet (5) comprises a carbon monoxide inlet (51) and an air inlet (52), the top wall of the gas mixing chamber (1) is provided with the carbon monoxide inlet (51), the side wall of the gas mixing chamber is symmetrically provided with the two air inlets (52), and the gas outlet (8) is positioned at the bottom of the gas mixing chamber (1);

the gas blending chamber (1) further comprises a blending fan (7) for stirring mixed gas, and the blending fan (7) is arranged inside the gas blending chamber (1) and is positioned between the gas inlet (5) and the gas outlet (8);

the carbon monoxide airflow entering the gas blending chamber (1) through the carbon monoxide inlet (51) is vertical to the air airflow entering the gas blending chamber (1) through the air inlet (52), the air airflows on the two sides of the gas blending chamber (1) are horizontal and coaxial, and vertical upward vortex airflows are formed through a bottom blending fan (7);

the side wall of the combined exposure cabin (3) is provided with a through hole (11), and the oronasal contamination cup (4) penetrates through the through hole (11) to be communicated with the combined exposure cabin (3) in a sealing way;

the oronasal contamination cup (4) comprises a cylindrical cup body (16), a cylindrical cavity body (15) and a conical cavity body (13), wherein the cylindrical cup body (16), the cylindrical cavity body (15) and the conical cavity body (13) are sequentially connected with the door body (17), the door body (17) is positioned on the outer side of the through hole (11), the conical cavity body (13) is positioned on the inner side of the through hole (11), and the cylindrical cavity body (15) and the cylindrical cup body (16) are connected in a nested manner;

opening (6) for set up in bar opening (14) on conical cavity (13), the size of bar opening (14) suits with the ear of rat.

2. The rat noise and carbon monoxide integrated exposure contamination experiment device according to claim 1, wherein a feces storage groove (19) is detachably connected to the bottom of the oronasal contamination cup (4).

3. The rat noise and carbon monoxide integrated exposure contamination experiment device according to claim 1, wherein a feces storage groove (19) and a strip-shaped channel (18) axially extending along the feces storage groove (19) are formed in the cylindrical cup body (16).

4. The rat noise and carbon monoxide integrated combined exposure contamination experiment device according to claim 1, wherein a monitoring port (10) is further arranged on the side wall of the combined exposure cabin (3).

5. The rat noise and carbon monoxide integrated joint exposure contamination experiment device according to claim 1, further comprising a rubber stopper (20) matched with the exhaust port (12).

6. The rat noise and carbon monoxide integrated combined exposure contamination experiment device according to claim 1, wherein the combined exposure cabin (3) is a cylinder, the gas mixing chamber (1) and the noise generation chamber (2) are both located at the center of the combined exposure cabin (3), and the oronasal contamination cups (4) are uniformly distributed on the periphery of the combined exposure cabin (3).