CN102697573B - Device for trauma stress experiment - Google Patents
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- CN102697573B CN102697573B CN201210142232.1A CN201210142232A CN102697573B CN 102697573 B CN102697573 B CN 102697573B CN 201210142232 A CN201210142232 A CN 201210142232A CN 102697573 B CN102697573 B CN 102697573B
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Abstract
The invention relates to a device for a trauma stress experiment. The device for the trauma stress experiment comprises a box body and a control system. Slots are arranged on the inner lateral side of the box body, two ends of a longitudinal plate and two ends of a transverse plate are respectively in inserting connection with the slots, and the longitudinal plate and the transverse plate are intersected and matched. A fence and a through air are arranged on the inner side of a base plate of the box body, and a shake generator and a gas generator which are electrically connected with the control system are fixed on the outer side of the base plate. A collecting plate is arranged at the bottom of the box body, a loudspeaker is installed on one side wall of the box body, and a current stimulating drive is installed on the other side wall. A noise drive, a camera and a light stimulating source are installed on the top plate. The noise drive is electrically connected with the loudspeaker, and a current generator is electrically connected with the fence. The control system comprises a computer, a single chip microcomputer, a programmable logic device, a photoelectric isolating circuit, the current stimulating drive, a light source drive and the noise drive. The device for the trauma stress experiment can provide various traumatic stress environments, is conveniently suitable for experimental subjects with different sizes, simultaneously performs one stress mode or various stress modes, and can perform real-time analysis or ex-post analysis.
Description
Technical field
The present invention relates to a kind of experimental apparatus, particularly a kind of device for trauma stress experiment.
Background technology
The non-specific general reaction that body occurs when being subject to various internal and external environment factor and stimulating stress be referred to.Cause stress stressor comprise physiologic factor, environmental factors and psychology, sociocultural factor, body carrys out self regulation to maintain the normal performance of the stable of environment and function by physiology and behavior cognitive reaction.But when stress not enough, excessively or overtime, will have a negative impact to body function.In recent years along with the generation of the outburst disaster event such as " 911 ", " SARS ", tsunami, earthquake, the war in Iraq, Wenchuan earthquake, major traffic accidents, the mental disorder that various trauma stress causes remains high.Current research confirmed already, excessive stress, particularly chronic stress can bring out or increase the weight of the neuropsychiatric disease such as many such as recurrent major depression and chronic stress and posttraumatic stress disorder (PTSD).Be exposed to for a long time in stressful situation and the crucial core of brain can be caused to roll into a ball such as atrophy of hippocampal, and and then bring out or increase the weight of some neuropsychiatric disorders that stress be correlated with.Increasing research shows stress to be a kind of important Control factors to brain structure and function, and therefore application experiment animal is necessary carrying out research at all levels very much.
Previously about the related experiment mainly direct wound that causes from limbs or adopt the incompatible simulation of multiple stress group trauma stress in various degree of Animal stress, such as combined stress pattern, Restraint Stress, under water stress, fatigue stress and single stimulation stress isotypes, these trauma stress patterns are when model copy, experimenter constantly directly contacts animal, very easily cause the anxiety of laboratory animal and affect experimental result, causing secondary stress; And judge at model, normally stress judge by means of the different behavioristics's testing tool separated later, lack ageing.
Simultaneously due to subjective judgment or hand-kept experimental data that experimental result is experimenter, lack effective measuring and analysis appraisal procedure, make these methods have time-consuming, interference is large, objectivity is poor, quantification degree is low, by defects such as artificial subjective factor interference are large.Particularly in drug research, need to observe medicine to impacts such as brain learning memory, cognitive function and emotions, and drug mechanism, efficacy of medicine observing, new drug research etc. need to meet the various behavioristics's training stimulating requirement and animal build specification, require that the basic physiological data demand of laboratory animal is accurately careful.At present, for avoiding experimental error excessive, reduce unnecessary accordingly stress, be the different modelling of adaptation and experimental design requirement, and different animal specifications need to buy different instrument, the special specification instrument of analogy mice, rat, rabbit, and existing instrument design is only for single animal training or making, cannot batch duplicating model, cause experimental work personnel to need the at substantial quality time, work and conventional efficient low.
Summary of the invention
In order to overcome the above-mentioned deficiency of prior art, the invention provides a kind of device for trauma stress experiment.It can be adapted to the experimental subject of different size easily, and what provide various wounds stress environment, realizes one or more stimulus modelities of Stress model and carries out simultaneously, and can in real time or postmortem analysis.
Technical scheme of the present invention is:
For the device of stress tests, this device comprises casing, control system, described casing is electrically connected with control system, wherein, box inside face is provided with the slot for installing compoboard, stringer board in compoboard and transverse slat are that decussation agrees with, stringer board, the two ends of transverse slat respectively with the slot grafting in box inside face, the fence by electric current and several passages are provided with inside the base plate of casing, the outside of base plate is mounted with the vibration machine and gas generator that are electrically connected with control system, the bottom of casing is provided with the drawing and pulling type collecting board for collecting animal excrements, one sidewall of casing installs loudspeaker, another sidewall of casing installs galvanism driver, mounting of roof plate has the noise driver be electrically connected with control system, photographic head, photostimulation source, noise driver is electrically connected with loudspeaker, one electrode of galvanism driver is electrically connected with fence,
Control system comprises computer, single-chip microcomputer, programmable logic device, photoelectric isolating circuit, galvanism driver, light source drive, noise driver, wherein, computer is used for video data and image and sends instruction to single-chip microcomputer, and controls the switch of photographic head and accept the video signal that photographic head provides; Single-chip microcomputer is connected with computer by RS232 serial ports, accepts the various instructions that computer sends, and performs corresponding stimulation according to its instruction; Single-chip Controlling PLD performs selects galvanism waveform, the instruction of control and drive system; PLD provides different stimulus waveforms, for galvanism driver, light source drive, noise driver provide control signal for galvanism; Galvanism driver connects different electrodes respectively, and band moving electrode does different stimulations; The analog signal isolating of the digital controlled signal of programmable logic device (CPLD) and single-chip microcomputer and current driver, light source drive, noise driver is opened by Phototube Coupling, reduces to interfere with each other; Power module provides power supply for control system.
Compoboard is sound insulation, light tight medical organic plates.
Described casing is rectangle, and its forward and backward medial surface arranges several slots longitudinally running through its medial surface equidistantly, and the intermediate symmetry of left and right medial surface is provided with the slot that longitudinally runs through medial surface.
Described slot is the projection protruding from the medial surface being positioned at this slot.
The end face of described compoboard is provided with the depression corresponding with slot.
The stringer board of described compoboard is provided with the groove under shed.
Photostimulation source is high-powered LED lamp.
Described photographic head is connected with computer by USB interface.
4 electrodes that galvanism driver connects, wherein first Electrode connection fence, producing constant current stimulates, and makes fence produce constant current, constant voltage stimulates; Second Electrode connection hairbrush produces tactual stimulation; 3rd Electrode connection vibrator produces vibrations to stimulate, and the 4th Electrode connection gas generator, produces micro-tapping.
Galvanism driver can also be done as required stress tests different, connect different electrodes respectively, band moving electrode does different stimulations, as done the experiment etc. of the emergent stimulation such as optical, electrical, fiery.
The present invention makes full use of the high-tech such as modern biotechnology sensing, electronics, computer media, in acousto-optic-electric shielding with in the laboratory of strict Temperature and Humidity Control, to Animal stress and the behavior in process stress carry out long-time noiseless monitoring analysis, whole record and rough estimates process are completed by computer controlled automatic completely, overcome current animal model preparation process behavior acquisition method to shortcomings such as " secondary interference stress " that animal produces.Adopt the behavior analysis system of image analysis, can carry out more comprehensively animal behavior activity, meticulous, accurate, objective appraisal.Adopt modern mathematical tool to analyze experimental data, obtain brand-new characteristics index.To the collaborative research of different side parameter can more complete reflection objectively stress relation between ambient source and stress, combine the mensuration of the index such as biochemical, find its inherent mechanism, preventing and treating for stress in clinical and wartime provides more scientific theoretical foundation.Meanwhile, several little training chests of apparatus of the present invention, can combine according to the size of animal figure, applied range, and can carry out by many animals simultaneously, improve conventional efficient.Compoboard described in apparatus of the present invention is sound insulation, opaque medical organic plates, has the advantage of being convenient to process.
The development of computer technology and be applied as behavioristics and detect and bring wide prospect, and the quantification that the mathematical tool such as fuzzy mathematics, neutral net is behavioral indexes provides powerful guarantee, make it no longer to be confined to traditional only to the simple statistics of direct observed result, and likely excavate novel more can the characteristic index of concentrated expression laboratory animal behavioristics change.Applicant fully to consider in experimentation current stress parameter, give the Selecting parameter of wide region, experiment proves, adopting apparatus of the present invention can greatly improve stress the experimental applications of intelligence.
Test with common behavior in the market and compare, native system is based upon on area of computer aided image and other automatic technology bases, and arrange according to the environment peculiarity and timeliness of trauma stress, the present invention has the following advantages:
1. real-time objective is strong, obtaining information amount is large, index incubation period and the errors number of main use can be provided, and utilize image procossing, pattern recognition, the technology such as infrared also can extract and comprise laboratory animal time and distance, motion and the index such as settling time, curve plotting figure, can directly video monitoring, analysis, reduce the interference in observation process, the stability of effective guarantee animal model and effectiveness, scientific degree is high.
2. automatization, high, the objective standardization of intelligence degree are good, simple to operate, and experimenter does not need long-term praxiology research experience, has advantage simple to operate, and experimenter directly observes animal activity situation when testing by video.
3. system configuration is cheap, popular higher, may be used for neuroscience experimentation field.
4. can the behavioral indexes of the according to circumstances multiple quantification of synchronous recording, such as gather the position of one or more animal different time in one or more region, speed, the time of staying, movement locus, the indexs such as move distance, the data obtained is careful, accurate;
5. pair image data is carried out online or is analysed in depth afterwards afterwards, easily finds more novel more convictive behavioristics New Set (experiment parameter);
6. easily set up detection, the criterion of trauma stress, get rid of the interference of artificial subjective factor;
7. physiological parameter can be combined with behavioral indexes and carry out synchronous recording, analysis, this is also one of development trend of modern praxiology research.
This device is verified through zoopery, it can be used for that list or various wounds stress animal model are set up, the research field such as the pharmacology of different model and physiological Study in learning and memory and neural field, special is being analyze under the on-line analysis and line of laboratory animal, avoid the error usually occurred in hand-kept, be also beneficial to data compilation thereafter and analysis.The record of automatization and rough estimates, for we providing mass data, to the rational parameter of evaluation and selection of laboratory animal motion, are also the emphasis that this experiment is paid close attention to.In special time period, the accumulated counts of the rate travel of laboratory animal is as index, and can reflect its motion conditions preferably, the present invention has experimental result good stability, and experimental implementation is flexible, saves the advantage of manpower, funds.
The present invention according to the needs of trauma stress experiment, can also add various sensor, the data information acquisitions such as such as muscle tone, electrocardio, Skin Resistance, such as can lead physiological acquisition system or activity remote sensing etc. more arrange integration with the existing collection of laboratory.
Accompanying drawing explanation
Fig. 1 is this apparatus structure schematic diagram;
Fig. 2 is Control system architecture figure;
Fig. 3 is control system workflow diagram;
Fig. 4 A is this device box structural representation;
Fig. 4 B is the structural representation of stringer board;
Fig. 5 A is the original picture of experimental animal rat when accepting trauma stress;
The contoured image of Fig. 5 B and the experimental animal rat synchronous process when accepting trauma stress;
Fig. 6 is the instant barycenter trajectory figure of trauma stress experimental rat;
Fig. 7 is the instant grey scale centre of gravity scattergram of trauma stress experimental rat;
Fig. 8 is that system is to the schematic diagram of each movement rate of experimental rat according to time variations;
Fig. 9 is experimental rat each rate travel counting statistics rectangular histogram in the unit time;
Figure 10 is the time dependence change schematic diagram of experimental rat sports level M value;
Figure 11 is experimental rat sports level value time dependence change schematic diagram.
In figure, 1 is casing, and 2 is top board, and 3 is collecting board, and 4 is transverse slat, and 5 is stringer board, and 6 is base plate, and 7 is passage, and 8 is fence, and 9 is groove, and 10 is LED, and 11 is noise driver, and 12 is photographic head, and 13 is slot, and 14 is computer, and 15 is control system.
Detailed description of the invention
The present embodiment main devices is as follows:
Single-chip microcomputer adopts macrocrystalline series monolithic STC89C52;
CPLD adopts altera corp EPM7128SLC84-15;
Photostimulation source adopts high-power LED lamp pearl;
Loudspeaker are 3 watts of conventional 2 cun of bright basin Full-frequency trumpets;
Noise driver adopts power amplifier driver, as power amplifier and audio amplifier etc., carries out power amplification to the audio signal of computer export, drives sound horn.The chip adopted is the category D amplifier chip PAM8303D that Power Analog Microelectronics company of the U.S. produces.This external noise Drive Module also arranges 4 Omron relay G3VM-201G1, for controlling the switch of four casing audio signals.The control signal that single-chip microcomputer exports exports the switch of control four relays via CPLD.
The i-e031 infrared pick-up head that infrared camera adopts Shenzhen Jia Shina numeral science and technology company limited to produce, 1,200 ten thousand pixels, 640*480 resolution;
Power module, uses 2 Switching Power Supplies: upper hamming latitude Switching Power Supply SW-480W, exports 110V, input voltage---AC220V ± 10%; The JMD35-A switch power source output voltage 5V(2A that the extra large scientific and technological group of letter produces) ± 12V(1A)
On-off circuit employs the relay G3VM-201G1 that Japanese Omron Corp produces, and single-chip microcomputer exports control signal via CPLD, the break-make of control relay.
Gas generator adopts gas to export as 200g/cm
2~ 500g/cm
2air pump;
Vibrating actuator adopts frequency of vibration to be 100Hz, and input power is the electromagnetic shaker of 10 ~ 15W;
Casing is made up of lighttight resin material, size 40cm × 20cm × 15cm;
Fence is made up of stainless steel tube, diameter 5mm, interval 10mm.
See Fig. 1 to Fig. 4, the device for stress tests comprises casing, control system 15, and described casing 1 is electrically connected with control system.Wherein, box inside face is provided with the slot 13 for installing compoboard, stringer board 5 in compoboard agrees with in decussation with transverse slat 4, stringer board, the two ends of transverse slat respectively with the slot grafting in box inside face, the fence 8 by electric current and several passages 7 are provided with inside the base plate of casing, the outside of base plate 6 is mounted with the vibration machine and gas generator (not indicating in figure) that are electrically connected with control system, the bottom of casing is provided with the drawing and pulling type collecting board 3 for collecting animal excrements, one sidewall of casing installs loudspeaker (not indicating in figure), another sidewall of casing installs current feedback circuit (not indicating in figure), top board 2 is provided with the noise driver 11 be electrically connected with control system, photographic head 12, photostimulation source (LED 10), loudspeaker are electrically connected with noise driver 11, and an electrode of galvanism driver is electrically connected with fence.
Describedly stress to be made up of sound insulation, light-proof material by casing, top board 2 installs photostimulation source, photographic head, noise driver, described photostimulation source can be LED 10, case sidewall installs loudspeaker, several rustless steel fence by electric current are provided with at the bottom of case, the diameter 5mm of rustless steel fence described in the present embodiment, the interval 10mm between each rustless steel fence, bottom joins a drawing and pulling type excreta collection plate 3 again; The forward and backward sidewall of casing is equidistant is provided with several slots 13 longitudinally running through its sidewall, and the intermediate symmetry of left and right medial surface is provided with the slot that longitudinally runs through medial surface, compoboard is combined with casing slot lubricate, firm.Housing interior volume is divided into several little training chests by this device stringer board, transverse slat, and as 4,6,8,10 etc., the present embodiment is 4.Slot is the projection protruding from the medial surface being positioned at this slot, the groove that the both ends of the surface of compoboard are corresponding with slot respectively.Described compoboard is sound insulation, opaque medical organic plates (resin material), and there is a groove that Open Side Down 9 centre of stringer board, for the agreeing with of transverse slat, compoboard is combined convenient, firmly with casing.Install galvanism driver at case rear wall, box top center bulb mounting and infrared photography head unit, be equipped with drawing and pulling type excreta collection plate at the bottom of case, this collecting board is divided into multiple zonule (corresponding each little training chest) according to sequence number.
Compoboard is different according to the body size of animal, be plugged in slot, be combined into be applicable to different animals build specification and different number animal can multiple little training chest simultaneously, the size of little training chest is different and arrange according to the figure of animal.Described little training chest is applicable to the training of common small sized laboratory animal, as: be applicable to mice or be applicable to rat or be applicable to Cavia porcellus or be applicable to the little training chest of rabbit etc.Compoboard requires adopt sound insulation and have certain robustness material, as light tight medical organic plates (resin material) etc., effectively can avoid the mutual interference of many animals when respective little training chest training and struggle damaging, ensure the accuracy of measured data.
Control system comprises computer 14, single-chip microcomputer, programmable logic device, photoelectric isolating circuit, galvanism driver, light source drive, noise driver.Control system controls the switch of photographic head on the one hand and accepts its video signal exported, and date processing, calculates various parameter; Various stimulus modelity instruction is sent to single-chip microcomputer on the other hand by RS232 serial ports; Computer is used for computer and is used for video data and video image and sends instruction to single-chip microcomputer, and controls the switch of photographic head and accept the video signal that photographic head provides; Single-chip microcomputer is connected with computer by RS232 serial ports, accepts the various instructions that computer sends, and performs corresponding stimulation according to its instruction.The program finished writing in advance is placed in single-chip microcomputer, the various instructions read compare by single-chip microcomputer, find the type (e.g., Sound stimulat, electricity irritation, vibrations stimulation etc.) of instruction, completeer backward programmable logic device exports selects galvanism waveform or control signal; Programmable logic device provides different stimulus waveforms, for galvanism driver, light source drive, noise driver provide control signal for galvanism; The analog signal isolating of the digital controlled signal of programmable logic device CPLD and single-chip microcomputer and current driver, light source drive, noise driver is opened by Phototube Coupling, reduces to interfere with each other; Power module provides power supply for control system, and power module has relay protection circuit, can provide ± 5V, ± 12V, 110V voltage (± 5V, ± 12V, ± 110V).See Fig. 2, galvanism driver directly produces the work of stimulus signal drive electrode, and producing constant current stimulation, micro-tapping, tactual stimulation and vibrations stimulates.Electrode 1 produces constant current to stimulate, and makes fence produce constant current, constant voltage stimulates; Electrode 2 connects hairbrush and produces tactual stimulation, and electrode 2 is with electric brush to do tactual stimulation; Electrode 3 connects electromagnetic shaker (the present embodiment adopts frequency of vibration to be 100Hz, and input power is the vibrator of 10 ~ 15W) and produces vibrations stimulation, and electrode 4 connects gas generator, and (the present embodiment adopts gas to export as 200g/cm
2~ 500g/cm
2air pump), produce micro-tapping.Light source drive is made up of LED switch circuit (the relay G3VM-201G1 that on-off circuit uses Japanese Omron Corp to produce, single-chip microcomputer exports control signal via CPLD, the break-make of control relay), can control the disconnection of four bulbs respectively and close.Noise driver controls the opening and closing of power amplifier and audio amplifier by on-off circuit, noise driver is also connected with audio interface, for loudspeaker provide audio signal, noise driver provides background white noise by loudspeaker and sharply frightens Sound stimulat, and audio interface is connected with computer.4 infrared cameras connect computer by USB interface, the video signal that the switch of computer control photographic head and reception photographic head provide.
Described control system interface hommization, operating instruction is detailed, directive function enriches, and mainly gathers and analyze the data of casing thecamera head, carries out video camera tracking technique, achieve the automatization of experimentation, the image processing means such as binaryzation, comb filtering, image sharpening are utilized to Information Monitoring simultaneously, the original image collected is converted into contour images, using profile diagram as record and storing mode, not only data volume greatly reduces, and also can be further statistical disposition and lays a solid foundation.Observe according to us, the contour images of laboratory animal, substantially intactly can reflect laboratory animal Morphological Features at that time, meet requirement of experiment.The focus point of laboratory animal of this system by calculating simultaneously, the movement rate of direct experiment with computing animal and record its event trace, have good real-time, data statistics is also relatively simple and reliable.Comprise by testing the data analysis that can carry out at present: quiescent time, movement time, movement locus figure, liveness analytical curve, freezing total degree, each freezing time point, each freezing persistent period, freezing total time, the freezing time accounts for the percentage ratio of total time, mobility is correlated with: total experimental period, total distance (cm), over-all velocity (cm/s), the time in each stage, distance and average speed, the many indexs such as animal movement type.
Table 1. is for the basic configuration of trauma stress experimental provision
Time by experimenter sets experiment formally, but not laboratory animal enters that time of trauma stress casing starts, concrete operation step is as follows:
1. laboratory animal is put into cage, adapt to 30 minutes, any stimulation is not carried out to laboratory animal.
2. start to carry out trauma stress or measurement, first select trauma stress type.
A) if select galvanism, first set current intensity, current square wave frequency, stimulation time, after setting, point starts, constant current then inside circuit board stimulates driver will export the current square wave of setting intensity frequency to fence, simultaneous computer starts experiments of measuring animal quiescent time, movement time, movement locus figure, liveness analytical curve, freezing total degree, each freezing time point, each freezing persistent period, freezing total time, the freezing time accounts for the percentage ratio of total time, mobility is correlated with: total experimental period, total distance (cm), over-all velocity (cm/s), the time in each stage, distance and average speed, the many indexs (parameter is all identical below) such as animal movement type, and it is dynamic by image window observation experiment animal, the time one of setting arrives, driver stops output current square wave, computer stops timing.
B) if selective light stimulates, first set light intensity and stimulation time, then beginning is put, light source drive is opened switch and is lighted LED bulb in cage, and simultaneous computer starts experiments of measuring animal parameters, and shows with curve, and it is dynamic by image window observation experiment animal, the time one of setting arrives, and light source generator closes LED bulb, and computer stops timing.
C) if select noise to stimulate, first select the noise (noise of several selection can be had) stimulated, setting volume and stimulation time, point starts, then the loudspeaker in cage will release noise, and simultaneous computer starts experiments of measuring animal parameters, and it is dynamic by image window observation experiment animal, the time one of setting arrives, and loudspeaker stop, and stops timing.
D) stimulate if select to blow, select the source of the gas stimulated, strong and the stimulation time of setting gas, click and start, then tracheal strips has air release, and simultaneous computer starts experiments of measuring animal parameters, and it is dynamic by image window observation experiment animal, the time one of setting arrives, and air feed stops, and computer stops timing.
E) if select vibrations to stimulate, select vibrating mode, setting vibroseis intensity and stimulation time, click and start, then casing vibrations, simultaneous computer starts experiments of measuring animal parameters, and dynamic by image window observation experiment animal, the time one of setting arrives, and vibrations stop, and stops timing.
F) if select tactual stimulation, select haptic mode, setting sense of touch intensity and stimulation time, click and start, hairbrush rotates, and simultaneous computer starts experiments of measuring animal parameters, and it is dynamic by image window observation experiment animal, the time one of setting arrives, and hairbrush rotates and stops, and computer stops timing.
Different demarcation strips is partitioned into different experiments animal space, can select different measurement types, and starts to measure simultaneously, also can separately measure.
3rd step: measure and terminate, data are preserved automatically, and get back to calculating owner interface, can continue to measure, also can exit.
The a certain stimulation of trauma stress is decided by intensity, frequency and stimulation time, by experimenter experimentally wound degree required by animal difference and set.
Embodiment
SD rat is male, 9, and body weight (180 ± 35) g, is provided by this institute animal experimental center.By this system realize putting under house arrest adding electric shock manufacture trauma stress obstacle rat model (note: operational measure parameter by experimenter by software operation Interface Control, but not laboratory animal enters test box determines).
Apply this system operation procedures as follows: select electricity irritation at component computer interface, setting circuit current intensity, current square wave frequency, stimulation time, constant current: 8mA, time-histories: 10s, time delay: 0, string number: 100, sets after after parameter, point starts, constant current then inside circuit board stimulates driver will export the current square wave of setting intensity frequency to fence, and observe rat by image window dynamic, the time one of setting arrives, driver stops output current square wave, and software stops timing.By SD rat the morning 8:00-12:00 and afternoon 2:00-6:00 put into System cabine, carry out electric shock for three days on end.In modeling process, carry out of software support system to the image of experimental rat gathers, processes and record: rat original picture (see Fig. 5 A), through process such as image binaryzation, comb filtering, edge extractings, complete contoured (see Fig. 5 B).Contoured image is conducive to extracting rat center of gravity to record its movement locus change (see figure 6) and pace of change (see figure 7).
This system can realize rough estimates laboratory animal movement rate rectangular histogram: this experiment setting by movement rate by 0,1,2,3,5,8,13,21,34,55,89, more than 144() pixels/0.5s carries out cumulative numeration respectively and percentage ratio is added up.The mass activity region of laboratory animal in metabolic cage is 320 × 240pixel, selects above-mentioned rate of displacement can intactly comprise laboratory animal motor behavior, and is conducive to carrying out labor, see Fig. 8 and Fig. 9 to different motion speed.
Table 2 trauma stress obstacle rat motor horizontal M value result of calculation
(note: x: meansigma methods, s: standard deviation)
In different time sections: in the time period of 5min, 10min, 15min, 20min, 25min, 30min, 40min, 60min, 1.5h, 2h, 4h, 6h and 8h, 9 wound experiment stress rats (are numbered n1 ... 7, x, s) mean motion speed.We think this value can this time period laboratory animal of concentrated expression preferably suffer stress after the sports level of dissimilar activity (be mainly divided into: static, move, stroll, wash and dress), see Figure 10, Figure 11, table 2.
Three. carrying out preliminary classification according to movement rate to laboratory animal motor behavior: the real-time performance of control experiment animal, according to the rate variation situation under different motion situation shown by monitoring system, is four kinds by its kinestate rough segmentation to study further:
1. static (Freeze): 0pixel/0.5s; (non-displacement, remains static substantially, comprises sleep, and rest also comprises catatonic type attitude and fixes)
2. combing (combing): 1pixel/0.5s; (without walking, but have head, limbs, body movable, main manifestations is combing activity, and simple feed and drinking-water activity often also comprise wherein)
3. stroll (walking): 2-8pixel/0.5s; (walking, seeks with looking for food and strolls character with general)
4. escape (fast walking): 13-144pixel/0.5s; (walking fast, under having more the situation of the uneasy quick escape caused now)
These four kinds of compositions are in a certain special time period, and the percentage ratio of shared whole movement rate is different.We are according to the variation tendency of sports level value M, and select the type of sports percentage ratio of 25 minutes to study, in this time period, laboratory animal sports level tends towards stability, can think laboratory animal enter foreign environment after the investigatory reaction phase.Experimentally the difference of animal various above-mentioned motion percentage within a certain set time, can describe more accurately its kinestate, be conducive to further research.This device also achieves simultaneously to be added up respective regions and time, number of times.
Claims (8)
1. the device for trauma stress experiment, it is characterized in that: this device comprises casing, control system, described casing is electrically connected with control system, wherein, box inside face is provided with the slot for installing compoboard, stringer board in compoboard and transverse slat are that decussation agrees with, stringer board, the two ends of transverse slat respectively with the slot grafting in box inside face, the fence by electric current and several passages are provided with inside the base plate of casing, the outside of base plate is mounted with the vibration machine and gas generator that are electrically connected with control system, the bottom of casing is provided with the drawing and pulling type collecting board for collecting animal excrements, one sidewall of casing installs loudspeaker, another sidewall of casing installs galvanism driver, mounting of roof plate has the noise driver be electrically connected with control system, photographic head, photostimulation source, noise driver is electrically connected with loudspeaker, one electrode of galvanism driver is electrically connected with fence, compoboard is sound insulation, light tight medical organic plates,
Control system comprises computer, single-chip microcomputer, programmable logic device, photoelectric isolating circuit, galvanism driver, light source drive, noise driver, wherein, computer is used for video data and image and sends instruction to single-chip microcomputer, and controls the switch of photographic head and accept the video signal that photographic head provides; Single-chip microcomputer is connected with computer by RS232 serial ports, accepts the various instructions that computer sends, and performs corresponding stimulation according to its instruction; Single-chip Controlling PLD performs selects galvanism waveform, the instruction of control and drive system; PLD provides different stimulus waveforms, for galvanism driver, light source drive, noise driver provide control signal for galvanism; Galvanism driver connects different electrodes respectively, and band moving electrode does different stimulations; The analog signal isolating of the digital controlled signal of programmable logic device (CPLD) and single-chip microcomputer and current driver, light source drive, noise driver is opened by Phototube Coupling, reduces to interfere with each other; Power module provides power supply for control system.
2. the device for trauma stress experiment according to claim 1, it is characterized in that: described casing is rectangle, its forward and backward medial surface arranges several slots longitudinally running through its medial surface equidistantly, and the intermediate symmetry of left and right medial surface is provided with the slot that longitudinally runs through medial surface.
3. the device for trauma stress experiment according to claim 1, is characterized in that: described slot is the projection protruding from the medial surface being positioned at this slot.
4. the device for trauma stress experiment according to claim 1, is characterized in that: the end face of described compoboard is provided with the depression corresponding with slot.
5. the device for trauma stress experiment according to claim 1, is characterized in that: be provided with the groove under shed in the stringer board of described compoboard.
6. the device for trauma stress experiment according to claim 1, is characterized in that: photostimulation source is high-powered LED lamp.
7. the device for trauma stress experiment according to claim 1, is characterized in that: described photographic head is connected with computer by USB interface.
8. the device for trauma stress experiment according to claim 1, is characterized in that: 4 electrodes that galvanism driver connects, and wherein first electrode produces constant current stimulates, and makes fence produce constant current, constant voltage stimulates; Second Electrode connection hairbrush produces tactual stimulation; 3rd Electrode connection vibrator produces vibrations to stimulate, and the 4th Electrode connection gas generator, produces micro-tapping.
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| CN201210142232.1A CN102697573B (en) | 2012-05-09 | 2012-05-09 | Device for trauma stress experiment |
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| CN201210142232.1A CN102697573B (en) | 2012-05-09 | 2012-05-09 | Device for trauma stress experiment |
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| CN103040523A (en) * | 2012-12-07 | 2013-04-17 | 中国人民解放军第四军医大学 | Animal noise stimulation system |
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| CN109718481A (en) * | 2017-10-31 | 2019-05-07 | 深圳先进技术研究院 | Ultrasonic neuromodulation system, method and the storage medium for treating addictive disorders |
| CN109044271B (en) * | 2018-06-25 | 2021-06-11 | 江南大学 | Sleep recording bin and recording method |
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| CN110251266B (en) * | 2019-06-25 | 2021-06-04 | 中国人民解放军陆军军医大学 | Rat noise and carbon monoxide integrated combined exposure contamination experimental device |
| CN111202606A (en) | 2020-02-24 | 2020-05-29 | 中国人民解放军陆军军医大学 | Multi-condition multi-simulation-environment universal accurate animal injury platform |
| CN115721437B (en) * | 2022-11-16 | 2023-09-12 | 武汉科技大学 | Device and method for modeling chronic fatigue syndrome composite factors of mice |
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