CN102697573A - Device for trauma stress experiment - Google Patents
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- CN102697573A CN102697573A CN2012101422321A CN201210142232A CN102697573A CN 102697573 A CN102697573 A CN 102697573A CN 2012101422321 A CN2012101422321 A CN 2012101422321A CN 201210142232 A CN201210142232 A CN 201210142232A CN 102697573 A CN102697573 A CN 102697573A
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Abstract
The invention relates to a device for a trauma stress experiment. The device for the trauma stress experiment comprises a box body and a control system. Slots are arranged on the inner lateral side of the box body, two ends of a longitudinal plate and two ends of a transverse plate are respectively in inserting connection with the slots, and the longitudinal plate and the transverse plate are intersected and matched. A fence and a through air are arranged on the inner side of a base plate of the box body, and a shake generator and a gas generator which are electrically connected with the control system are fixed on the outer side of the base plate. A collecting plate is arranged at the bottom of the box body, a loudspeaker is installed on one side wall of the box body, and a current stimulating drive is installed on the other side wall. A noise drive, a camera and a light stimulating source are installed on the top plate. The noise drive is electrically connected with the loudspeaker, and a current generator is electrically connected with the fence. The control system comprises a computer, a single chip microcomputer, a programmable logic device, a photoelectric isolating circuit, the current stimulating drive, a light source drive and the noise drive. The device for the trauma stress experiment can provide various traumatic stress environments, is conveniently suitable for experimental subjects with different sizes, simultaneously performs one stress mode or various stress modes, and can perform real-time analysis or ex-post analysis.
Description
Technical field
The present invention relates to a kind of experimental apparatus, particularly a kind of device that is used for the trauma stress experiment.
Background technology
Stress be meant that body is receiving the non-specific general reaction that is occurred when various internal and external environment factors stimulate.Cause stress stressor comprise physiologic factor, environmental factors and psychology, sociocultural factor, body comes the normal performance of self regulation with the stable and function of environment in keeping through physiology and behavior cognitive reaction.But when stress be not enough, excessively or overtime, will have a negative impact to body function.Along with the generation of sudden disaster events such as " 911 ", " SARS ", tsunami, earthquake, the war in Iraq, Wenchuan earthquake, major traffic accidents, the mental disorder that various trauma stress cause is high in recent years.Current research confirmed already, over-drastic stress, particularly chronic stress can bring out or increase the weight of many such as recurrent major depression and chronic stress and posttraumatic stress disorder neuropsychiatric diseases such as (PTSD).Long term exposure can cause the crucial nuclear of brain group such as atrophy of hippocampal in stressful situation, and and then brings out or increase the weight of the neuropsychiatric disorders that some stress be correlated with.Increasing research shows to be a kind of important regulation and control factor to brain structure and function, so the application experiment animal is necessary carrying out research at all levels very much.
Previously about animal stress related experiment mainly be the wound that directly causes, or adopt the incompatible simulation of multiple stress group trauma stress in various degree from limbs; Such as the combined stress pattern; Restraint Stress, under water stress, fatigue stress and single stimulation stress isotype, these trauma stress patterns are when model copy, the experimenter constantly directly contacts animal; Very easily cause the anxiety of laboratory animal and influence experimental result, cause the secondary stress; And judge at model, normally stress come later to judge, lack ageing by means of different behavioristics's testing tool of separating.
Simultaneously because experimental result is experimenter's subjective judgment or hand-kept experimental data; Lack and effectively measure and analyzing evaluation method; Make these methods have time-consuming, disturb big, objectivity is poor, the quantification degree is low, receives artificial subjective factor to disturb defectives such as big.Particularly aspect drug research; Need to observe medicine to influences such as brain learning memory, cognitive function and emotions; And drug mechanism, efficacy of medicine observing, new drug research etc. need meet behavioristics's training of various stimulation requirements and animal build specification, require the basic physiological data demand of laboratory animal careful accurately.At present, excessive for avoiding experimental error, minimizing is accordingly unnecessary stress; Be different modellings of adaptation and experimental design requirement, and the different animal specification need be bought different instruments, the special specification instrument of analogy mice, rat, rabbit; And existing instrument design is only to single animal training or making; Can't the batch duplicating model, cause the experimental work personnel to need the labor quality time, work and conventional efficient are low.
Summary of the invention
In order to overcome the above-mentioned deficiency of prior art, the present invention provides a kind of device that is used for the trauma stress experiment.It can be adapted to the experimental subjecies of different sizes easily, and what multiple wound was provided stress environment, and realization stress be carried out by one or more stimulus modelities of pattern simultaneously, and can be in real time or postmortem analysis.
Technical scheme of the present invention is:
Be used for the device that stress test, this device comprises casing, control system, and said casing is electrically connected with control system; Wherein, The box inside face is provided with the slot that is used for the installation and combination plate, and stringer board in the compoboard and transverse slat are decussation to be agreed with, and pegs graft with the slot of box inside face respectively in the two ends of stringer board, transverse slat; The base plate inboard of casing is provided with can be through the fence and several passages of electric current; The outside of base plate is mounted with vibration machine and the gas generator that is electrically connected with control system, and the bottom of casing is provided with the drawing and pulling type collecting board that is used to collect animal excrements, and a sidewall of casing is installed loudspeaker; Another sidewall of casing is installed the galvanism driver, and top board is equipped with noise driver, photographic head, the photostimulation source that is electrically connected with control system; The noise driver is electrically connected with loudspeaker; One electrode of galvanism driver is electrically connected with fence;
Control system comprises computer, single-chip microcomputer, programmable logic device, photoelectric isolating circuit, galvanism driver, light source drive, noise driver; Wherein, Computer is used for video data and image and sends instruction to single-chip microcomputer, and the switch of control photographic head and accept the video signal that photographic head provides; Single-chip microcomputer is connected with computer through the RS232 serial ports, accepts the various instructions that computer sends, and carries out corresponding the stimulation according to its instruction; The Single-chip Controlling PLD is carried out and is selected the galvanism waveform, the instruction of control driver; PLD is that galvanism provides different stimulus waveforms, for galvanism driver, light source drive, noise driver provide control signal; The galvanism driver connects different electrodes respectively, drives electrode and does different stimulations; Photoelectricity is isolated the analogue signal of the digital controlled signal of programmable logic device (CPLD) and single-chip microcomputer and current driver, light source drive, noise driver is kept apart, and reduces to interfere with each other; Power module is that control system provides power supply.
Compoboard is sound insulation, light tight medical organic plates.
Said casing is a rectangle, and its forward and backward medial surface is provided with the slot that several vertically run through its medial surface equidistantly, the centre of left and right medial surface be symmetrically arranged with one vertically run through medial surface slot.
Said slot is the convexity that protrudes in the medial surface that is positioned at this slot.
The end face of said compoboard is provided with the depression corresponding with slot.
The stringer board of said compoboard is provided with the groove under shed.
The photostimulation source is a high-powered LED lamp.
Said photographic head is connected with computer through USB interface.
4 electrodes that the galvanism driver connects, wherein first electrode connects fence, and producing constant current stimulates, and fence generation constant current, constant voltage are stimulated; Second electrode connects hairbrush and produces tactual stimulation; The 3rd electrode connects vibrator and produces the vibrations stimulation, and the 4th electrode connects gas generator, and producing gas stimulates.
What the galvanism driver can also be done as required stress test difference, connects different electrodes respectively, drives electrode and does different stimulations, as do the experiment etc. of emergent stimulations such as light, electricity, fire.
The present invention makes full use of high-tech such as modern bio-sensing, electronics, computer media; In the laboratory of acousto-optic electric screen and strict Temperature and Humidity Control; To animal stress and behavior in stress process carry out long-time noiseless monitoring analysis; Whole record and rough estimates process are accomplished by computer controlled automatic fully, have overcome current animal model and have prepared the shortcomings such as " secondary interference stress " that process behavior acquisition method produces animal.Adopt behavioristics's analytical system of image analysis, can carry out more comprehensive, meticulous, accurate, objective appraisal the animal behavior activity.Adopt modern mathematical tool that experimental data is analyzed, obtain brand-new characteristic index.To the collaborative research of different side parameter can more complete reflection objectively stress ambient source and stress between relation, ins conjunction with the mensuration of indexs such as biochemistry, find its inherent mechanism, for clinical with wartime stress prevent and treat the more theoretical foundation of science be provided.Simultaneously, the little training chest of several of apparatus of the present invention can make up according to animal figure's size, applied range, and can carry out simultaneously by many animals, improved conventional efficient.The described compoboard of apparatus of the present invention is sound insulation, opaque medical organic plates, and the advantage of being convenient to process is arranged.
Development of computer be applied as behavioristics and detect and to bring wide prospect; And mathematical tools such as fuzzy mathematics, neutral net are the quantification of behavioristics's index powerful guarantee is provided; Make it no longer to be confined to traditional, and might excavate the novel characteristic index that more can concentrated expression laboratory animal behavioristics changes only to direct observation result's simple statistics.The applicant in experimentation, fully considered current stress parameter, the parameter that gives wide region is selected, experiment showed, adopt apparatus of the present invention can greatly improve stress intelligence experimental applications.
With in the market common behavior test relatively, native system is to be based upon on area of computer aided image and other automatic technology bases, and is provided with according to the environment peculiarity and timeliness of trauma stress, the present invention has the following advantages:
Real-time objective property strong, obtain and contain much information; The index incubation period and the errors number of main use can be provided; And utilize Flame Image Process, pattern recognition, technology such as infrared also can extract to comprise indexs such as laboratory animal time and distance, motion and tranquillization time, curve plotting figure, directly video monitoring, analysis reduces the interference in the observation process; Effectively guarantee the stability and the effectiveness of animal modeling, scientific degree is high.
2. automatization, intelligent degree are high, objective standardization is good, simple to operate, and the experimenter does not need secular behavioristics research experience, and advantage simple to operate is arranged, and the experimenter can pass through video direct observation animal activity situation when experiment.
3. system configuration is cheap, and is popular higher, can be used for neuroscience experimentation field.
4. behavioristics's index of the multiple quantification of synchronous recording according to circumstances, such as the position of gathering one or more animals different time in one or more zones, speed, the time of staying, movement locus, indexs such as move distance, the gained data are careful, accurate;
5. carry out online or back in-depth analysis afterwards to image data, find novel more convictive behavioristics New Set (experiment parameter) easily;
6. set up detection, the criterion of trauma stress easily, get rid of the interference of artificial subjective factor;
7. can physiological parameter and behavioristics's index be combined and carry out synchronous recording, analysis, this also is one of development trend of modern behavioristics research.
This device is through the zoopery checking; It can be used for the research fields such as pharmacology and physiological Study of different models in single or the foundation of multiple trauma stress animal model, learning and memory and the neural field; Especially be analysis under the on-line analysis of laboratory animal and line; Avoid the error that usually occurs in the hand-kept, also be beneficial to thereafter data compilation and analysis.The record of automatization and rough estimates provide mass data for us, to the assessment and the selection reasonable parameter of laboratory animal motion, also are the emphasis that this experiment is paid close attention to.As index, can reflect its motion conditions with the accumulated counts of the rate travel of laboratory animal in the special time period preferably, the present invention has the experimental result good stability, and experimental implementation is flexible, saves the advantage of manpower, funds.
The present invention can also add various pick offs according to the needs of trauma stress experiment, such as data information acquisitions such as muscle tone, electrocardio, Skin Resistances, can such as leading physiology acquisition system or activity remote sensing etc. integration be set with breadboard existing collection more.
Description of drawings
Fig. 1 is this apparatus structure sketch map;
Fig. 2 is the control system structure chart;
Fig. 3 is the control system workflow diagram;
Fig. 4 A is this device box structural representation;
Fig. 4 B is the structural representation of stringer board;
Fig. 5 A is the original picture of experimental animal rat when accepting trauma stress;
The contoured image of Fig. 5 B and the experimental animal rat Synchronous Processing when accepting trauma stress;
Fig. 6 is the instant barycenter trajectory figure of trauma stress experimental rat;
Fig. 7 is the instant grey scale centre of gravity scattergram of trauma stress experimental rat;
Fig. 8 sketch map that to be system change according to the time each movement rate of experimental rat;
Fig. 9 is each rate travel counting statistics rectangular histogram of experimental rat in the unit interval;
Figure 10 is that the time dependence of experimental rat sports level M value changes sketch map;
Figure 11 is that experimental rat sports level value time dependence changes sketch map.
Among the figure, 1 is casing, and 2 is top board, and 3 is collecting board, and 4 is transverse slat, and 5 is stringer board, and 6 is base plate, and 7 is passage, and 8 is fence, and 9 is groove, and 10 is the LED lamp, and 11 is the noise driver, and 12 is photographic head, and 13 is slot, and 14 is computer, and 15 is control system.
The specific embodiment
The present embodiment main devices is following:
Single-chip microcomputer adopts macrocrystalline series monolithic STC89C52;
CPLD adopts the EPM7128SLC84-15 of altera corp;
The high-power LED lamp pearl is adopted in the photostimulation source;
Loudspeaker are 3 watts 2 cun bright basin full range loudspeaker commonly used;
The noise driver adopts the power amplifier driver, like power amplifier and audio amplifier etc., the audio signal of computer output is carried out power amplification, drives sound horn.The chip that adopts is the D class power amplifier chip PAM8303D that U.S. Power Analog Microelectronics company produces.This external noise Drive Module also is provided with 4 Omron relay G3VM-201G1, is used to control the switch of four casing audio signals.The control signal of single-chip microcomputer output is via the switch of four relays of CPLD output control.
The i-e031 infrared pick-up head that infrared camera adopts Shenzhen Jia Shina numeral science and technology company limited to produce, 1,200 ten thousand pixels, 640*480 resolution;
Power module uses 2 Switching Power Supplies: go up hamming latitude Switching Power Supply SW-480W, output 110V, input voltage---AC220V ± 10%; JMD35-A switch power source output voltage 5V (2A) ± 12V (1A) that the extra large scientific and technological group of letter produces
The relay G3VM-201G1 that on-off circuit has used Japanese Omron Corp to produce, single-chip microcomputer is exported control signal via CPLD, the break-make of control relay.
Gas generator adopts gas to be output as 200g/cm
2~ 500g/cm
2Air pump;
It is 100Hz that vibrating actuator adopts frequency of vibration, and input power is the electromagnetic shaker of 10～15W;
Casing is to be processed by lighttight resin material, size 40cm * 20cm * 15cm;
Fence is processed by stainless steel tube, diameter 5mm, 10mm at interval.
Referring to Fig. 1 to Fig. 4, the device that is used for testing comprises casing, control system 15, and said casing 1 is electrically connected with control system.Wherein, The box inside face is provided with the slot 13 that is used for the installation and combination plate; Stringer board 5 in the compoboard is decussation with transverse slat 4 to be agreed with; Peg graft with the slot of box inside face respectively in the two ends of stringer board, transverse slat, the base plate inboard of casing is provided with can be through the fence 8 and several passages 7 of electric current, and the outside of base plate 6 is mounted with vibration machine and the gas generator (not indicating among the figure) that is electrically connected with control system; The bottom of casing is provided with the drawing and pulling type collecting board 3 that is used to collect animal excrements; One sidewall of casing is installed loudspeaker (not indicating among the figure), and another sidewall of casing is installed current feedback circuit (not indicating among the figure), and top board 2 is equipped with noise driver 11, photographic head 12, photostimulation source (the LED lamp 10) that is electrically connected with control system; Loudspeaker are electrically connected with noise driver 11, and an electrode of galvanism driver is electrically connected with fence.
Saidly stress constitute by sound insulation, light-proof material by casing; Top board 2 is installed photostimulation source, photographic head, noise driver, and said photostimulation source can be LED lamp 10, and the case sidewall is installed loudspeaker; Being provided with at the bottom of the case can be through several rustless steel fence of electric current; The diameter 5mm of the said rustless steel fence of present embodiment, the interval 10mm between each rustless steel fence, the bottom is joined a drawing and pulling type excreta collection plate 3 again; Equidistant several slots that vertically runs through its sidewall 13 that is provided with of the forward and backward sidewall of casing, the centre of left and right medial surface be symmetrically arranged with one vertically run through medial surface slot, it is lubricated, firmly that compoboard is combined with the casing slot.This device is slit into several little training chests with stringer board, transverse slat with the box house spatial, and as 4,6,8,10 etc., present embodiment is 4.Slot is the convexity that protrudes in the medial surface that is positioned at this slot, the both ends of the surface of compoboard respectively with the slot corresponding groove.Said compoboard is sound insulation, opaque medical organic plates (resin material), and the groove 9 that the centre of stringer board has Open Side Down is used for and the agreeing with of transverse slat, and it is convenient, firmly that compoboard is combined with casing.At the case rear wall galvanism driver is installed, bulb and infrared photography head unit are installed in the center, box top, are equipped with drawing and pulling type excreta collection plate at the bottom of the case, and this collecting board is divided into a plurality of zonules (corresponding each little training chest) according to sequence number.
The bodily form of compoboard according to animal varied in size, be plugged in the slot, be combined into a plurality of little training chests simultaneously that are applicable to different animals build specification and different number animals, the size of little training chest is according to different setting of figure of animal.Said little training chest is applicable to the training of common small sized laboratory animal, as: be applicable to mice or be applicable to rat or be applicable to Cavia porcellus or be applicable to the little training chest of rabbit etc.Compoboard requires to adopt sound insulation and has certain robustness material; Like light tight medical organic plates (resin material) etc.; Can effectively avoid the phase mutual interference and struggle breaking-up etc. of many animals when little training chest is separately trained, guarantee the accuracy of measuring data.
Control system comprises computer 14, single-chip microcomputer, programmable logic device, photoelectric isolating circuit, galvanism driver, light source drive, noise driver.Control system is controlled the switch and the video signal of accepting its output of photographic head on the one hand, and date processing, calculates various parameters; Send various stimulus modelity instructions through the RS232 serial ports to single-chip microcomputer on the other hand; Computer is used for computer and is used for video data and video image and sends instruction to single-chip microcomputer, and the switch of control photographic head with accept the video signal that photographic head provides; Single-chip microcomputer is connected with computer through the RS232 serial ports, accepts the various instructions that computer sends, and carries out corresponding the stimulation according to its instruction.The program of finishing writing in advance is presented in the single-chip microcomputer; The various instructions that single-chip microcomputer will read compare; The type of searching instruction (as, sound stimulation, electricity irritation, vibrations stimulation etc.), relatively intacter back is exported to programmable logic device and is selected galvanism waveform or control signal; Programmable logic device is that galvanism provides different stimulus waveforms, for galvanism driver, light source drive, noise driver provide control signal; Photoelectricity is isolated the analogue signal of the digital controlled signal of programmable logic device CPLD and single-chip microcomputer and current driver, light source drive, noise driver is kept apart, and reduces to interfere with each other; Power module is that control system provides power supply, and power module has relay protection circuit, can provide ± 5V, and ± 12V, 110V voltage (± 5V, ± 12V, ± 110V).Referring to Fig. 2, the galvanism driver directly produces the work of stimulus signal drive electrode, and producing constant current stimulation, gas stimulation, tactual stimulation and vibrations stimulates.Electrode 1 produces constant current to stimulate, and makes fence produce constant current, constant voltage stimulation; Electrode 2 connects hairbrush and produces tactual stimulation, and electrode 2 drives hairbrush and does tactual stimulation; Electrode 3 connects electromagnetic shaker (it is 100Hz that present embodiment adopts frequency of vibration, and input power is the vibrator of 10～15W) and produces the vibrations stimulation, and electrode 4 connects gas generator, and (present embodiment adopts gas to be output as 200g/cm
2～500g/cm
2Air pump), producing gas stimulates.Light source drive is made up of LED on-off circuit (the relay G3VM-201G1 that on-off circuit uses Japanese Omron Corp to produce, single-chip microcomputer is exported control signal via CPLD, the break-make of control relay), can control the disconnection and the closure of four bulbs respectively.The noise driver is by the opening and closing of on-off circuit control power amplifier and audio amplifier; The noise driver also is connected with COBBAIF; For loudspeaker provide audio signal, the noise driver provides background white noise and sharp-pointed scaring sound to stimulate through loudspeaker, and COBBAIF is connected with computer.4 infrared cameras connect computer through USB interface, the video signal that the switch of computer control photographic head and reception photographic head provide.
The interface hommization of said control system, operating instruction is detailed, and directive function is abundant; Mainly be the data of gathering and analyze the transmission of casing photographic head, carry out the video camera tracking technique, realized the automatization of experimentation; Simultaneously Information Monitoring is utilized image processing means such as binaryzation, comb filtering, image sharpening, the original image that collects is converted into contour images, with profile diagram as the record and storing mode; Not only data volume greatly reduces, and also can be further statistical disposition and lays a solid foundation.Observe according to us, the contour images of laboratory animal can intactly reflect laboratory animal form characteristics at that time basically, satisfies requirement of experiment.This system is through the focus point of the laboratory animal that calculates simultaneously, and directly the movement rate of experiment with computing animal and its event trace of record have good real-time performance, and data statistics is also simple and reliable relatively.Comprise through testing the data analysis that can carry out at present: quiescent time; Movement time; Movement locus figure; The liveness analytical curve; The freezing total degree; Each freezing time point; Each freezing persistent period; Freezing total time; The freezing time accounts for the percentage ratio of total time; Mobility is relevant: total experimental period; Total distance (cm); Over-all velocity (cm/s); The time in each stage; Distance and average speed; Many indexs such as animal movement type.
Table 1. is used for the basic configuration of trauma stress experimental provision
Set the time that experiment formally begins by the experimenter, but not laboratory animal gets into that the time of trauma stress casing begins, the concrete operations step is following:
1. put laboratory animal into cage, adapt to 30 minutes, laboratory animal is not carried out any stimulation.
2. begin to carry out trauma stress or measure elder generation's selection trauma stress type.
A) if select galvanism; Set current intensity earlier; The electric current square wave frequency; Stimulation time, after configuring, the some beginning; Then the constant current of the circuit board the inside electric current square wave that stimulates driver to export to set the intensity frequency is to fence; It is relevant that simultaneous computer begins experiments of measuring animal quiescent time, movement time, movement locus figure, liveness analytical curve, freezing total degree, each freezing time point, each freezing persistent period, freezing total time, the freezing time accounts for percentage ratio, the mobility of total time: many indexs (following parameter is all identical) such as total experimental period, total distance (cm), over-all velocity (cm/s), time, distance and the average speed in each stage, animal movement type, and it is dynamic to pass through image window observation experiment animal, the time one of setting arrives; Driver stops the output current square wave, and computer stops timing.
B), set light intensity and stimulation time earlier, then beginning if selective light stimulates; Light source drive is opened switch and is lighted the LED bulb in the cage, and simultaneous computer begins experiments of measuring animal parameter, and with curve display; And can be dynamic through image window observation experiment animal; The time of setting one arrives, and light source generator is closed the LED bulb, and computer stops timing.
C) if select noise to stimulate, select the noise (several kinds of noises of selecting can be arranged) of stimulation earlier, set volume and stimulation time; The point beginning, then the loudspeaker in the cage will be emitted noise, and simultaneous computer begins experiments of measuring animal parameter; And can be dynamic through image window observation experiment animal; The time of setting one arrives, and loudspeaker stop, and stop timing.
D) if selecting to blow stimulates, select the source of the gas of stimulation, set the strong and stimulation time of gas; Click beginning, then in the trachea gas release is arranged, simultaneous computer begins experiments of measuring animal parameter; And can be dynamic through image window observation experiment animal; The time of setting one arrives, and air feed stops, and computer stops timing.
E) if select vibrations to stimulate, select vibrating mode, set vibroseis intensity and stimulation time, click beginning; Then casing vibrations, simultaneous computer begins experiments of measuring animal parameter, and can be dynamic through image window observation experiment animal; The time of setting one arrives, and vibrations stop, and stop timing.
F) if select tactual stimulation, select the sense of touch pattern, set sense of touch intensity and stimulation time; Click beginning, hairbrush rotates, and simultaneous computer begins experiments of measuring animal parameter; And can be dynamic through image window observation experiment animal; The time of setting one arrives, and hairbrush rotates and stops, and computer stops timing.
Different demarcation strips is partitioned into different experiments animal space, can select different measurement types, and begins simultaneously to measure, and also can separately measure.
The 3rd step: measure and finish, data are preserved automatically, and get back to computer master interface, can continue to measure, and also can withdraw from.
The a certain stimulation of trauma stress is to be decided by intensity, frequency and stimulation time, is set according to the difference of wound degree that laboratory animal requires by the experimenter.
Embodiment
The SD rat is male, and 9, body weight (180 ± 35) g is provided by this institute zoopery center.Realize putting under house arrest through this system and adding electric shock and make trauma stress obstacle rat model (annotate: the operational measure parameter, is determined but not laboratory animal gets into test box through the software operation Interface Control by the experimenter).
It is following to use this system's operating procedure: select electricity irritation at the component computer interface, set electric current current intensity, electric current square wave frequency, stimulation time; Constant current: 8mA, time-histories: 10s, time-delay: 0, string number: 100; After configuring after the parameter, some beginning, then the constant current of the circuit board the inside electric current square wave that stimulates driver to export to set the intensity frequency can be dynamic through image window observation rat to fence; The time of setting one arrives, and driver stops the output current square wave, and software stops timing.With the SD rat the morning 8:00-12:00 and afternoon 2:00-6:00 put into System cabine, carry out electric shock for three days on end.In the modeling process, software support system is to the gathering of the image of experimental rat, processing and record: rat original picture (referring to Fig. 5 A), through processing such as image binaryzation, comb filtering, edge extractings, accomplish contoured (referring to Fig. 5 B).The contoured image helps extracting the rat center of gravity and changes (see figure 6) and pace of change (see figure 7) to write down its movement locus.
This system can realize rough estimates laboratory animal movement rate rectangular histogram: this experiment set with movement rate by 0,1,2,3,5,8,13,21,34,55,89,144 (more than) add up respectively numeration and percentage ratio of pixels/0.5s adds up.The mass activity zone of laboratory animal in metabolic cage is 320 * 240pixel, selects for use above-mentioned rate of displacement can intactly comprise the laboratory animal motor behavior, and helps different motion speed is carried out labor, referring to Fig. 8 and Fig. 9.
The horizontal M value of table 2 trauma stress obstacle rat motor result of calculation
(annotate: x: meansigma methods, s: standard deviation)
At different time sections: 5min, 10min, 15min, 20min, 25min, 30min, 40min, 60min, 1.5h, 2h, 4h, 9 wound experiment stress rats (are numbered n1 in the time period of 6h and 8h ... 7, x, mean motion speed s).We think the concentrated expression preferably of this value should the time period laboratory animal suffer stress after the sports level of dissimilar activities (mainly be divided into: static, move, stroll, wash and dress), see Figure 10, Figure 11, table 2.
Three. according to movement rate the laboratory animal motor behavior is carried out preliminary classification: the real-time performance of control experiment animal according to the rate variation situation that monitoring system showed under the different motion situation, is four kinds so that further study with its kinestate rough segmentation:
1. static (Freeze): 0pixel/0.5s; (non-displacement remains static basically, comprises sleep, has a rest to comprise that also the catatonic type attitude fixes)
2. combing (combing): 1pixel/0.5s; (do not have walking, but have head, limbs, body movable, mainly show as the combing activity, take food merely and the drinking-water activity often also comprises wherein)
3. stroll (walking): 2-8pixel/0.5s; (walking, having looks for food seeks and the general character of strolling)
4. escape (fast walking): 13-144pixel/0.5s; (walking fast has more under the situation of the present uneasy quick escape that causes)
These four kinds of compositions are in a certain special time period, and the percentage ratio of shared whole movement rate has nothing in common with each other.We are according to the variation tendency of sports level value M, select 25 minutes type of sports percentage ratio to study, and in this time period, the laboratory animal sports level tends towards stability, and can think the investigatory reaction phase after laboratory animal gets into foreign environment.According to the difference of laboratory animal shared percentage ratio of various above-mentioned motions in a certain set time, can describe more accurately its kinestate, help further research.This device has also been realized respective regions and time, number of times are added up simultaneously.
Claims (9)
1. one kind is used for the device that trauma stress is tested, and it is characterized in that: this device comprises casing, control system, and said casing is electrically connected with control system; Wherein, The box inside face is provided with the slot that is used for the installation and combination plate, and stringer board in the compoboard and transverse slat are decussation to be agreed with, and pegs graft with the slot of box inside face respectively in the two ends of stringer board, transverse slat; The base plate inboard of casing is provided with can be through the fence and several passages of electric current; The outside of base plate is mounted with vibration machine and the gas generator that is electrically connected with control system, and the bottom of casing is provided with the drawing and pulling type collecting board that is used to collect animal excrements, and a sidewall of casing is installed loudspeaker; Another sidewall of casing is installed the galvanism driver, and top board is equipped with noise driver, photographic head, the photostimulation source that is electrically connected with control system; The noise driver is electrically connected with loudspeaker; One electrode of galvanism driver is electrically connected with fence;
Control system comprises computer, single-chip microcomputer, programmable logic device, photoelectric isolating circuit, galvanism driver, light source drive, noise driver; Wherein, Computer is used for video data and image and sends instruction to single-chip microcomputer, and the switch of control photographic head and accept the video signal that photographic head provides; Single-chip microcomputer is connected with computer through the RS232 serial ports, accepts the various instructions that computer sends, and carries out corresponding the stimulation according to its instruction; The Single-chip Controlling PLD is carried out and is selected the galvanism waveform, the instruction of control driver; PLD is that galvanism provides different stimulus waveforms, for galvanism driver, light source drive, noise driver provide control signal; The galvanism driver connects different electrodes respectively, drives electrode and does different stimulations; Photoelectricity is isolated the analogue signal of the digital controlled signal of programmable logic device (CPLD) and single-chip microcomputer and current driver, light source drive, noise driver is kept apart, and reduces to interfere with each other; Power module is that control system provides power supply.
2. the device that is used for the trauma stress experiment according to claim 1, it is characterized in that: compoboard is sound insulation, light tight medical organic plates.
3. the device that is used for the trauma stress experiment according to claim 1; It is characterized in that: said casing is a rectangle; Its forward and backward medial surface is provided with the slot that several vertically run through its medial surface equidistantly, the centre of left and right medial surface be symmetrically arranged with one vertically run through medial surface slot.
4. the device that is used for the trauma stress experiment according to claim 1, it is characterized in that: said slot is the convexity that protrudes in the medial surface that is positioned at this slot.
5. the device that is used for the trauma stress experiment according to claim 1, it is characterized in that: the end face of said compoboard is provided with the depression corresponding with slot.
6. the device that is used for the trauma stress experiment according to claim 1 is characterized in that: be provided with the groove under shed in the stringer board of said compoboard.
7. the device that is used for the trauma stress experiment according to claim 1, it is characterized in that: the photostimulation source is a high-powered LED lamp.
8. the device that is used for the trauma stress experiment according to claim 1 is characterized in that: said photographic head is connected with computer through USB interface.
9. the device that is used for the trauma stress experiment according to claim 1 is characterized in that: 4 electrodes that the galvanism driver connects, and wherein first electrode produces constant current stimulates, and fence generation constant current, constant voltage are stimulated; Second electrode connects hairbrush and produces tactual stimulation; The 3rd electrode connects vibrator and produces the vibrations stimulation, and the 4th electrode connects gas generator, and producing gas stimulates.
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| CN201210142232.1A CN102697573B (en) | 2012-05-09 | 2012-05-09 | Device for trauma stress experiment |
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| CN109718481A (en) * | 2017-10-31 | 2019-05-07 | 深圳先进技术研究院 | Ultrasonic neuromodulation system, method and the storage medium for treating addictive disorders |
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