CN110214568A - A kind of grafting seedlings-growing method of cassava - Google Patents
A kind of grafting seedlings-growing method of cassava Download PDFInfo
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- CN110214568A CN110214568A CN201910664452.2A CN201910664452A CN110214568A CN 110214568 A CN110214568 A CN 110214568A CN 201910664452 A CN201910664452 A CN 201910664452A CN 110214568 A CN110214568 A CN 110214568A
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- grafting
- scion
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- cassava
- marrow
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of grafting seedlings-growing method of cassava, include the following steps: (1) Root-stock selection, (2) scion selection, (3) grafting, (4) managing after grafting, (5) nursery, (6) field management.The present invention is grafted using stock marrow space, simplifies grafting work flow, effectively breaks " Space Time " limitation in tradition grafting, can be carried out seedling stem grafting operation indoors completely, be improved grafting operating efficiency；Grafting seedling stem seedling management is carried out under the conditions of controllable light, epidemic disaster indoors, improves the efficiency of management.
Description
Technical field
The present invention relates to technical field of crop cultivation, and in particular to a kind of grafting seedlings-growing method of cassava.
Background technique
Grafting, is one of artificial propagation and Genetic improvement method of crop；Cassava is important the torrid zone/subtropical zone starch and makees
Object can be improved cassava adaptability, resistance and yield by the new strain of cassava that grafting obtains and be greatly improved, and be a kind of
Practical new technology.Cassava stock and the graft union tissue of scion further break up, and gradually healing survives tissue, are formed new
Plant；Increase carbohydrate anabolism, development using its powerful Photosynthetic Efficiency, growth is vigorous, and tree crown, branches and leaves distribution will
More rationally, be conducive to the formation of storage root, expand the accumulation with starch.
Existing cassava graft technology mainly carries out scion grafting in field and bud connects, and has certain limitation on working space
Property；If number of patent application is a kind of engrafting method of stem soft hollow plant of CN200710049751.2, following skill is disclosed
Art feature: " selection thickness, maturity almost the same scion and stock, are whittled into gradient for the grafting end of scion and stock respectively
Identical inclined-plane, with an insertion scion center marrow of label, stock center marrow is inserted into other end, makes the oblique of scion and stock
Face tightly coincide, and tightly wraps up securely graft union with bandage, seals the upper end fracture of scion ", the program has two:
1. stock material needs plantation or last year perennial root plant ahead of time, is managed to materials and bring more resource consumptions；2. with small
The fixed scion of bamboo stick and stock material, operating process difficulty are big, relatively complicated.
Therefore, how cassava seed stems grafted, simplify techniqueflow, improve grafting operating efficiency, guarantee higher survive
Rate is current urgent problem.
Summary of the invention
The purpose of the present invention is to provide a kind of grafting seedlings-growing methods of cassava, can effectively break cassava graft technology operation
" Space Time " limits in process, realizes that cassava seed stems grafting, nursery plantation application simplify techniqueflow, graft material facilitated to supervise
It surveys, management, improve survival rate and working efficiency, grafting seedling stem, seedling can be produced in batch, be conducive to grinding for cassava new germ plasm
The high yield and high quality of hair and promotion cassava plant development, upgrading synergy.
To solve the above problems, the present invention adopts the following technical scheme that:
A kind of grafting seedlings-growing method of cassava, includes the following steps:
(1) Root-stock selection: stock chooses seedling stem aging, intact without wound, bud eye, surface sterilization, after drying, with disinfection knife
Tool cuts 10-15cm, and both ends notch is smooth, smooth, digs out the sea of marrow from stock upper end incision with disinfecting forceps, small iron hook
Silk floss tissue is sure not to damage tissue on the inside of marrow, forms scion insertion space, for use；
(2) scion selects: seedling stem aging, intact without wound, bud eye is chosen in scion, and scion diameter is slightly than stock marrow tissue
The big 2-3mm of diameter is emptied, surface sterilization after drying, cuts 7-10cm with disinfection cutter, both ends notch is smooth, smooth, with disinfection
Cutter exposes smooth xylem, tries not to injure xylem, for use in scion lower end band girdle, removal epidermal tissue 2-3cm；
(3) graft: the scion wound that step (2) obtains dips the processing of plant exogenous hormone mixed liquor, is inserted into step
Suddenly the stock marrow space position without spongy tissue that (1) obtains, slightly firmly compresses, and scion is closely tied with stock junction
It closes；With grafting dedicated film bandage will in succession wound dressing it is secured, sealed from scion upper cut-out, will from top to bottom graft seedling stem
It is sealed with film bandage, only stays stock inferior end notch；
(4) managing after grafting: the material that step (3) grafts is placed in 24.0 DEG C of temperature, relative humidity 40.0%-
In 50.0% environment for 24 hours；
(5) nursery: nursery is carried out using the graft material that step (4) obtain, 3/4 cuttage of stock part is entered into sand bed, temperature
24.0 DEG C -27.0 DEG C of degree, 90.0% or more relative humidity, shading are ventilated；After 10-14 days, the germination feelings of scion are checked
Condition, and film bandage is needled in time, auxiliary emergence；After 25-30 days, grafting part organization healing situation is checked, solve membrane removal in time and tie up
Band guarantees the good development of regeneration strain；
(6) field management: the new branches and leaves of scion are formed completely, and the root system of stock is sturdy, flourishing, can be planted regeneration at this time
Strain transplanting field, carries out out cave, upright plantation, and growing season management is identical as conventional seedling Cultivate administration.
Further, in step (1), the depth for digging out marrow spongy tissue is 2-3cm.
Further, in step (1) or step (2), the surface sterilization uses 50% thiophanate methyl wettable powder
700 times of liquid carry out.
Further, in step (3), the plant exogenous hormone mixed liquor by following quality-volumetric concentration substance group
At: 6-BA 5.0mg/ml and IBA 100.0mg/L.
The present invention has the advantages that compared with prior art
(1) it is grafted using stock marrow space, simplifies grafting work flow, effectively break " Space Time " in tradition grafting
Limitation can carry out seedling stem grafting operation indoors completely, improve grafting operating efficiency；Controllable light, epidemic disaster item indoors
Grafting seedling stem seedling management is carried out under part, improves the efficiency of management.
(2) using cassava stock marrow space graft, reduce the mechanical wounding to stock and scion, improve stock with
The binding force of scion, it is more effective to accelerate grafting part organization healing time (3- in advance in conjunction with the use that plant exogenous hormone mixes
5 days) and improve survival rate 15%-30%；
(3) this cassava graft technology can produce cassava grafting seedling stem in batches, form seedling breeding scale, it is new to be conducive to cassava
The research and development of germplasm and high yield and high quality, the upgrading synergy of promotion cassava plant development.
Specific embodiment
Below with reference to embodiment and test, the invention will be further described.
Embodiment 1
A kind of grafting seedlings-growing method of cassava, includes the following steps:
(1) stock chooses seedling stem aging, intact without wound, bud eye, with 50% thiophanate methyl wettable powder, 700 times of liquid
Surface sterilization is carried out, after drying, cuts 10-15cm (band 5-7 bud eye) with disinfection cutter, both ends notch is smooth, smooth, with disinfection
Tweezers, small iron hook dig out the spongy tissue of marrow from stock upper end incision, and depth is about 2-3cm, are sure not to damage on the inside of marrow
Tissue forms scion insertion space, for use；
(2) seedling stem aging, intact without wound, bud eye is chosen in scion, and scion diameter slightly empties diameter than stock marrow tissue
Big 2-3mm carries out surface sterilization with 50% thiophanate methyl wettable powder, 700 times of liquid, after drying, cuts 7- with disinfection cutter
10cm (band 3-5 bud eye), both ends notch is smooth, smooth, with disinfection cutter in scion lower end band girdle, removal epidermal tissue 2-3cm,
Expose smooth xylem, tries not to injure xylem, for use；
(3) scion wound dips plant exogenous hormone mixed liquor (plant exogenous hormone mixed liquor is dense by following quality-volume
The material composition of degree: 6-BA 5.0mg/ml and IBA 100.0mg/L) processing, it is inserted into stock marrow (no spongy tissue) spatial portion
Position, slightly firmly compresses, and scion is combined closely with stock junction；With grafting dedicated film bandage will in succession wound dressing it is secured,
It is sealed from scion upper cut-out, grafting seedling stem is sealed with film bandage from top to bottom, only stays stock inferior end notch；
(4) material grafted is placed in 24.0 DEG C of temperature, in the environment of relative humidity 40.0%-50.0% for 24 hours；
(5) nursery processing is carried out using the graft material that step (4) obtain, 3/4 cuttage of stock part is entered into sand bed, temperature
24.0 DEG C -27.0 DEG C, 90.0% or more relative humidity is shaded, ventilated；After 10-14 days, the germination of scion is checked,
And film bandage is needled in time, auxiliary emergence；After 25-30 days, checks grafting part organization healing situation, solves membrane removal bandage in time,
Guarantee the good development of regeneration strain；
(6) the new branches and leaves of scion are formed completely, and the root system of stock is sturdy, flourishing, regeneration plant can be transplanted field at this time
Between, cave, upright plantation are carried out out, growing season management is identical as conventional seedling Cultivate administration.
In order to illustrate technical effect of the invention, applicant carried out planting experiment below and comparative tests.
One, planting experiment
Following different Cassava Cultivars are grafted in the method for the embodiment of the present invention 1, are done with the seedling stem of wild cassava
Scion, each kind graft 30 plant, count survival rate after 45 days, the results are shown in Table 1:
Table 1: the cassava of different cultivars grafts situation
| Kind | SC205 | SC201 | SC124 | GR891 | GR911 | GR4 | GR5 |
| Graft survival rate (%) | 93.3% | 100% | 93.3% | 96.7% | 100% | 96.7% | 96.7% |
Two, comparative test
Using SC205 as test material, following control group is set and test group carries out test, counts survival rate after 45 days, tries
Test that the results are shown in Table 2.
Control group: with reference to a kind of engrafting method of stem soft hollow plant of CN200710049751.2, by embodiment 1
Step (1)-(3) replacement are as follows:
(1) spray aging, without the intact wild cassava of wound, bud eye, the work of clip 5~6cm of length, 2 bud eyes of band are chosen
Scion is whittled into inclined-plane in one end base portion far from bud eye；
(2) stock essentially identical with scion thickness, maturity is selected, stock stem is (40~50cm) in place
Top is cut off, shear plane is whittled into inclined-plane identical with scion；
(3) scion wound dips plant exogenous hormone mixed liquor (plant exogenous hormone mixed liquor is dense by following quality-volume
The material composition of degree: 6-BA 5.0mg/ml and IBA 100.0mg/L) processing, with an insertion scion center marrow of label, separately
One insertion stock center marrow after so that the inclined-plane of scion and stock is tightly coincide, is tightly wrapped up graft union securely with bandage,
Seal the fracture of scion upper end.
In addition to the above steps, remaining step is identical with the embodiment of the present invention 1.
Test group: doing scion with the seedling stem of wild cassava, using method identical with the embodiment of the present invention 1.
Table 2: the grafting survival situation of different disposal
| Group | The graft union time (day) | Graft survival rate (%) |
| Control group | 30 | 63.3 |
| Test group | 26 | 93.3 |
As shown in Table 2, compared with prior art, the present invention more effectively accelerates the grafting part organization healing time and mentions
The high survival rate of grafting.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (4)
1. a kind of grafting seedlings-growing method of cassava, which comprises the steps of:
(1) Root-stock selection: stock chooses seedling stem aging, intact without wound, bud eye, and surface sterilization after drying, is cut with disinfection cutter
10-15cm is taken, both ends notch is smooth, smooth, digs out the sponge group of marrow from stock upper end incision with disinfecting forceps, small iron hook
It knits, is sure not to damage tissue on the inside of marrow, forms scion insertion space, for use；
(2) scion selects: seedling stem aging, intact without wound, bud eye is chosen in scion, and scion diameter is slightly emptied than stock marrow tissue
The big 2-3mm of diameter, surface sterilization after drying, cut 7-10cm with disinfection cutter, and both ends notch is smooth, smooth, with disinfection cutter
In scion lower end, band girdle, removal epidermal tissue 2-3cm, expose smooth xylem, try not to injure xylem, for use；
(3) graft: the scion wound that step (2) obtains dips the processing of plant exogenous hormone mixed liquor, is inserted into step (1)
The obtained stock marrow space position without spongy tissue, slightly firmly compresses, and scion is combined closely with stock junction；With transferring
Connect dedicated film bandage will in succession wound dressing it is secured, seal from scion upper cut-out, from top to bottom tie up with film grafting seedling stem
Band is sealed, and stock inferior end notch is only stayed；
(4) managing after grafting: the material that step (3) grafts is placed in 24.0 DEG C of temperature, relative humidity 40.0%-50.0%
Environment in for 24 hours；
(5) nursery: nursery is carried out using the graft material that step (4) obtain, 3/4 cuttage of stock part is entered into sand bed, temperature
24.0 DEG C -27.0 DEG C, 90.0% or more relative humidity is shaded, ventilated；After 10-14 days, the germination of scion is checked,
And film bandage is needled in time, auxiliary emergence；After 25-30 days, checks grafting part organization healing situation, solves membrane removal bandage in time,
Guarantee the good development of regeneration strain；
(6) field management: the new branches and leaves of scion are formed completely, and the root system of stock is sturdy, flourishing, can be moved regeneration plant at this time
Field is planted, cave, upright plantation are carried out out, growing season management is identical as conventional seedling Cultivate administration.
2. a kind of grafting seedlings-growing method of cassava according to claim 1, which is characterized in that described to dig out in step (1)
The depth of marrow spongy tissue is 2-3cm.
3. a kind of grafting seedlings-growing method of cassava according to claim 1, which is characterized in that step (1) or step (2)
In, the surface sterilization is carried out using 50% thiophanate methyl wettable powder, 700 times of liquid.
4. a kind of grafting seedlings-growing method of cassava according to claim 1, which is characterized in that in step (3), the plant
Exogenous hormone mixed liquor by following quality-volumetric concentration material composition: 6-BA 5.0mg/ml and IBA 100.0mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910664452.2A CN110214568B (en) | 2019-07-23 | 2019-07-23 | Cassava grafting seedling method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910664452.2A CN110214568B (en) | 2019-07-23 | 2019-07-23 | Cassava grafting seedling method |
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| CN110214568A true CN110214568A (en) | 2019-09-10 |
| CN110214568B CN110214568B (en) | 2022-03-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113853964A (en) * | 2021-10-08 | 2021-12-31 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Macadamia nut breeding method combining grafting and mutagenesis |
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|---|---|---|---|---|
| CN101103670A (en) * | 2007-08-07 | 2008-01-16 | 广西亚热带作物研究所 | Cultivating method for promoting cassava production |
| CN101103682A (en) * | 2007-08-07 | 2008-01-16 | 广西亚热带作物研究所 | Method for grafting stalk soft hollow plant |
| CN102150565A (en) * | 2011-04-14 | 2011-08-17 | 华侨大学 | Medulla combined distant grafting method |
| CN104160879A (en) * | 2014-09-18 | 2014-11-26 | 中国热带农业科学院热带作物品种资源研究所 | Cassava tender stem greenhouse grafting method |
| CN106134780A (en) * | 2015-04-21 | 2016-11-23 | 高仁丽 | A kind of engrafting method of cane soft hollow plant |
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2019
- 2019-07-23 CN CN201910664452.2A patent/CN110214568B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103670A (en) * | 2007-08-07 | 2008-01-16 | 广西亚热带作物研究所 | Cultivating method for promoting cassava production |
| CN101103682A (en) * | 2007-08-07 | 2008-01-16 | 广西亚热带作物研究所 | Method for grafting stalk soft hollow plant |
| CN102150565A (en) * | 2011-04-14 | 2011-08-17 | 华侨大学 | Medulla combined distant grafting method |
| CN104160879A (en) * | 2014-09-18 | 2014-11-26 | 中国热带农业科学院热带作物品种资源研究所 | Cassava tender stem greenhouse grafting method |
| CN106134780A (en) * | 2015-04-21 | 2016-11-23 | 高仁丽 | A kind of engrafting method of cane soft hollow plant |
Non-Patent Citations (2)
| Title |
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| 刘文瑞等: ""板栗嫁接苗培育及栽培技术"", 《陕西林业科技》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113853964A (en) * | 2021-10-08 | 2021-12-31 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Macadamia nut breeding method combining grafting and mutagenesis |
| CN113853964B (en) * | 2021-10-08 | 2023-02-28 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Macadamia nut breeding method combining grafting and mutagenesis |
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