CN110201047A - The preparation method of one breeder mistake boisiana extract - Google Patents
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Abstract
The present invention provides the preparation method that a breeder loses boisiana extract, and this method is to extract 70% alcohol reflux of fevervine medicinal material, recycles ethyl alcohol and is concentrated into no alcohol taste.Preparation method simple and stable of the present invention, preparation cost is low, and preparation efficiency is high；The fevervine extract for treating infantile malnutrition effect of preparation is good, and chicken has been pushed to lose boisiana extract in the development of clinical application and medicine preparation.
Description
Technical field
The present invention relates to drug extractive technique, an especially breeder loses the preparation method of boisiana extract.
Background technique
Chicken loses rattan, Chinese medicine name.For the herb of madder wort fevervine, summer harvests herb, dries.Its sweet in flavor, slight bitter,
It is mild-natured.With expelling wind and removing dampness, the effect of analgesic antidotal, promoting digestion and removing indigestion, activating blood circulation and reducing swelling.For rheumatalgia, traumatic injury, outside
Wound property pain, liver and gallbladder and stomach and intestine colic pain, indigestion, infantile malnutrition, bronchitis；It is used for dermatitis, eczema and sore swollen toxin outside.
Infantile malnutrition refers generally to due to improper feeding or a variety of sickness influences, gas-liquid consumption wound impaired so as to cause taste and
A kind of chronic disease formed.Clinic is with body syntexis, lusterless complexion, hair are withered, apathetic or irritated diet is extremely
Main feature is the common frdquently encountered disease of paediatrics, its disease incidence is in rise year by year trend in recent years.For infantile malnutrition, doctor trained in Western medicine at present
There is no specificity therapeutic method, is mostly adjusted with probiotics preparation.Fevervine is Rubiaceae herbaceous perennial vine plant, is
A kind of civil widely used Chinese traditional herbs in China, can be clinically used for treatment gastrointestinal tract disease, retention of food and drink, stagnation of the circulation of vital energy stomach duct and abdomen is swollen
Bitterly, indigestion etc. has been widely used in treating the clinical treatment of infantile malnutrition, significant in efficacy.
Fevervine treats the good effects of infantile malnutrition, because of a kind of fevervine treatment infantile malnutrition active ingredient of the invention
Optimum extracting method is of great significance to its clinical application and medicine preparation development.
Summary of the invention
The object of the present invention is to provide the preparation methods that a breeder loses boisiana extract.Preparation method of the present invention is simply steady
Fixed, preparation cost is low, and preparation efficiency is high；The fevervine extract for treating infantile malnutrition effect of preparation is good, has pushed chicken to lose rattan and has mentioned
Take object in the development of clinical application and medicine preparation.
Technical solution of the present invention: a breeder loses the preparation method of boisiana extract, and this method is to use fevervine medicinal material
70% alcohol reflux extracts, and recycles ethyl alcohol and is concentrated into no alcohol taste.
A breeder above-mentioned loses in the preparation method of boisiana extract, and the method is to take fevervine medicinal material, adds 6-10 times to measure
70% ethyl alcohol, refluxing extraction 2-5 times, first time 1-3h, remaining each 1h every time, filtration recycle ethyl alcohol and are concentrated into no alcohol taste.
A breeder above-mentioned loses in the preparation method of boisiana extract, and the method is to take fevervine medicinal material, adds 7-9 times to measure
70% ethyl alcohol, refluxing extraction 2-4 times, first time 1-2h, remaining each 1h every time, filtration recycle ethyl alcohol and are concentrated into no alcohol taste.
A breeder above-mentioned loses in the preparation method of boisiana extract, and the method is to take fevervine medicinal material, adds 8 times of amounts 70%
Ethyl alcohol, refluxing extraction 3 times, first time 1.5h, remaining each 1h every time, filtration recycles ethyl alcohol and is concentrated into no alcohol taste.
To verify effect of the invention, inventor has carried out following experiment:
Experimental example:
1 materials and methods
The preparation of each extract of 1.1 fevervines
The fevervine place of production: Sichuan
Fevervine water decoction extract: weighing fevervine medicinal material 500g, and 10 times of amount water is added to decoct three times, and 1.5 hours for the first time,
Each 1 hour of second and third time, filtration, merging filtrate are concentrated into about 167mL.
Fevervine water decoction-alcohol sedimentation extract: weighing fevervine medicinal material 500g, adds 10 times of amount water to decoct three times, first time 1.5
Hour, each 1 hour of second and third time filters, and merging filtrate is concentrated into about 350mL, adds appropriate amount of ethanol, makes ethanol content
70%, 24 hours are stood, filtration recycles ethyl alcohol, is concentrated into about 167mL.
Fevervine alcohol reflux extract: weighing fevervine medicinal material 500g, adds 8 times of 70% ethyl alcohol of amount, and refluxing extraction 3 times,
One time 1.5 hours, each 1 hour of second and third time, filtration recycled ethyl alcohol and is concentrated into no alcohol taste, is dissolved in water to about 167mL.
The every 1mL of above-mentioned three kinds of extracts is equivalent to 3.0g medicinal material.
1.2 experimental animal
KM mouse, SPF grades, 10.0~13.0g, 2~3 weeks, 120, half male and half female is tested by Chongqing Institute of Chinese Medicine
Institute of Botany provides.Production licence number: SCXK (Chongqing) 2017-0003.Zoopery is in Chongqing Institute of Chinese Medicine Chinese medicine
Pharmacology three-level laboratory carries out.
1.3 drugs and reagent
"Xingpi Yanger Granule" (specification: 2g/ bags, manufacturer: Jianxing Medical Industry Co Ltd, Guizhou produces, batch number:
20170929)；Hungry plain (Ghrelin) (specification: 48T, manufacturer: Elabscience Biotechnology Co.,
Ltd, lot number: E89FID4JNY)；Motilin (Motilin) (specification: 48T, manufacturer: Elabscience
Biotechnology Co., Ltd, lot number: Y14GR64HB)；Other reagents are that domestic analysis is pure.
1.4 key instrument
1.6 R centrifuge of Legend MACH (Thermo Scientific)；(Shanghai China, the section experiment of ST-360 microplate reader
System Co., Ltd)；ST-36W board-washing machine (Shanghai Kehua Experimental System Co., Ltd.)；RE-2000 A Rotary Evaporators (Shanghai
Virtuous laboratory apparatus Co., Ltd)；UW4200s electronic balance (Shimadzu International Trading Company Ltd)；(Shimadzu is international by Auw220 D
Trade Co., Ltd)；CLW-1020 water purification machine (Chongqing Gan Lai Instrument Ltd.).
2 experimental methods
2.1 groupings and administration
After mouse adaptive feeding one day, 12 groups are randomly divided into, every group 10, half male and half female is real for apositia mouse
It tests and is tested with intemperance of taking food mice with spleen deficiency.Two experiments are 6 groups, respectively Normal group, model control group, be amusing feeding youngster
Particle group, fevervine water decoction extract group, fevervine water decoction-alcohol sedimentation extract group and fevervine alcohol reflux extract group.It is normal right
Isometric purified water is given according to group, model control group, remaining each group gives corresponding drug, daily stomach-filling 1 time respectively.
The influence of 2.2 pairs of apositia mouse
In addition to Normal group, remaining each group bibliography [9-11] method modeling 14d (uses fish floss, milk powder, corn
Powder, soybean powder, white sugar, fresh hen egg, fresh fat meat are stirred evenly according to the ratio of 1:1:1:2:1:1.8:2, by moulding from molding jig
Type, after being dried with baking oven, 4 DEG C of refrigerations are raised for animal pattern)；14d is administered, weighs 2 times weekly.Fasting before last dose
It can't help water 14 hours, Yu Ci superficial temporal veins take blood 0.5mL/, and only 3000r/min is centrifuged 10min, separates serum, -20 DEG C freeze
It is to be measured.Serum Ghrelin detection is carried out using enzyme-linked immunization.Taking after blood each group to give relative medicine, (all drugs contain
10% carbon dust, 0.2% Arabic gum), cervical dislocation is put to death after 30 minutes, dissection, is removed stomach and is sprayed door to rectum tail portion, measures pylorus
To pigment front end distance and pylorus to ileocecus distance, calculate alvine pushing rate=pigment promote length/small intestine overall length ×
100%.
The influence of 2.3 pairs of intemperance of taking food mice with spleen deficiency
In addition to Normal group, and remaining each group bibliography method (stomach-filling 100% refined lard 0.4mL/10g, daily 1
It is secondary, violet cabbage is freely eaten) [12-14] modeling 10d；7d, the during which appearance sign of close observation mouse, and Weighing body is administered
Weight.It is deprived of food but not water before last dose 14 hours, superficial temporal veins take blood 0.5mL/ only before being administered in next day, 3000r/min centrifugation
10min, separate serum, -20 DEG C freeze it is to be measured.MT in serum L detection is carried out using enzyme-linked immunization.Each group is the same as " 2.2 " after taking blood
Measure alvine pushing rate；Spleen, thymus gland are removed, filter paper wipes excess blood away, and weighing calculates organ index=organ weights (g)/body
Weight (g) * 100%.
2.4 statistics and analysis
Experimental result is with mean+SDIt indicates.Using one-way analysis of variance, P < 0.05 is conspicuousness
Difference.Data processing is analyzed using SPSS13.0 editions softwares.
3 results and analysis
The influence of 3.1 pairs of apositia mouse weights
Compared with Normal group, model control group weight is substantially reduced (P < 0.01).
Compared with model group, administration only alcohol reflux extract group mouse weight increase (P < 0.05) in D7 days, remaining each group is not
See and significantly change, is administered D14 days, in addition to water decoction extract, remaining mouse weight dramatically increases (P < 0.01).It the results are shown in Table 1.
Table 1 to apositia mouse weight influence (N=10)
Note: compared with Normal group##P<0.01；Compared with model group*P < 0.05,**P<0.01。
Note:Compare with control,##P<0.01；Compare with model,*P<0.05,**P<0.01.
The influence of 3.2 pairs of apositia mouse alvine pushing rates and serum Ghrelin content
Compared with Normal group, model control group alvine pushing rate and serum Ghrelin content reduce (P < 0.01).
Compared with model group, each administration group alvine pushing rate significantly increases (P < 0.01), and serum Ghrelin content removes decocting
Remaining outer administration mouse of extract is significantly raised (P < 0.01).It the results are shown in Table 2
Table 2 to apositia mouse alvine pushing rate, serum Ghrelin content influence (N=10)
Note: compared with Normal group##P<0.01；Compared with model group,**P<0.01。
Note:Compare with control,##P<0.01；Compare with model,**P<0.01.
The influence of 3.3 pairs of intemperance of taking food mice with spleen deficiency general status
In addition to Normal group, it is dry and hard that excrement occurred at modeling 2~3 days mostly in remaining each group mouse, tiny；4th~5 day
There is thin, weight loss；It is shapeless to occur excrement at the 6th~7 day, hair is alarmmed in serious person loose stool, and chilly is tired sleeping in groups；7th
Occur crissum filth, weakness of limbs, at shape of crawling, movement, slow in reacting, hogback at~9 days；Under mouse weight is obvious within 10th day
Drop prompts modeling success.After giving drug therapy, each administration group mouse is gradually recovered in the 4th day general status of administration, shows as
Mobility increases, and reaction is quick, and fur, spontaneous activity are gradually brought to normally；Model control group is not affected significantly.
The influence of 3.4 pairs of intemperance of taking food mice with spleen deficiency weight
Compared with Normal group, model control group mouse weight is substantially reduced (P < 0.01).Compared with model control group,
Each extract of fevervine and "Xingpi Yanger Granule" can obviously increase mouse weight (P < 0.01~0.05).It the results are shown in Table 3.
Table 3 to intemperance of taking food mice with spleen deficiency weight influence (N=10)
Note: compared with Normal group,##P<0.01；Compared with model group,*P < 0.05,**P<0.01。
Note:Compare with control,##P<0.01；Compare with model,*P < 0.05,**P<0.01.
The influence of 3.5 pairs of intemperance of taking food mice with spleen deficiency thymus gland, spleen, thymus index and index and spleen index
Compared with Normal group, model control group mouse thymus, spleen weight, thymus index and index and spleen index reduce
(P < 0.01~0.05).
Compared with model control group, "Xingpi Yanger Granule", alcohol reflux extract can increase mouse thymus, spleen weight,
Thymus index, index and spleen index (P < 0.01~0.05), water decoction-alcohol sedimentation extract only increase mouse thymus weight (P < 0.05), decocting
Extract has no statistical difference.It the results are shown in Table 4.
Table 4 to intemperance of taking food mice with spleen deficiency thymus gland, spleen, thymus index and index and spleen index influence (N=10)
Note: compared with Normal group##P<0.01；Compared with model group,*P < 0.05,**P<0.01。
Note:Compare with control,##P<0.01；Compare with model,*P<0.05,**P<0.01.
The influence of 3.6 pairs of intemperance of taking food mice with spleen deficiency alvine pushing rates and MT in serum L content
Compared with Normal group, model control group alvine pushing rate and MT in serum L content are substantially reduced (P < 0.01).
Compared with model control group, each extract alvine pushing rate of fevervine is significantly raised (P < 0.01), and MT in serum L contains
Only "Xingpi Yanger Granule", water decoction-alcohol sedimentation extract, alcohol reflux extract significantly increase (P < 0.01) to amount, and water decoction extract is without statistics
Learn difference.It the results are shown in Table 5.
Table 5 to intemperance of taking food mice with spleen deficiency alvine pushing rate and MT in serum L content influence (N=10)
Note: compared with Normal group,##P<0.01；Compared with model group,**P<0.01。
Note:Compare with control##P<0.01；Compare with model,**P<0.01.
4 discuss
Traditional medicine thinks: although the infantile malnutrition cause of disease is had nothing in common with each other, mainly in taste, pathogenesis is spleen fortune for sick position
Stomach receives malfunction, and the later period often results in weakness of the spleen and the stomach.Traditional Chinese medicine is treated infantile malnutrition and is particular about so that " reason taste change stagnant, QI invigorating
Blood, born fluid " is that principle carries out diagnosis and treatment based on an overall analysis of the illness and the patient's condition [16].Fevervine is civil in terms for the treatment of infantile malnutrition to be had been widely used, and is treated
Effect is definite, its medicinal material is widely distributed in addition, has the advantage of " effect favorable rates is honest and clean " in terms for the treatment of infantile malnutrition, has good city
Field prospect.This research using three kinds of extracts of fevervine as research object, using cause of disease simulation carry out apositia model and
Model of spleen deficiency is established, and by observation the weight of animals, the state of mind, organ weights and index come evaluation model success or not, and is seen
Examine influence of the fevervine to the gastrointestinal hormones such as alvine pushing rate and Ghrelin, MTL level.
Modern experimental research shows that: in addition to apparent spleen deficiency symptom, body weight increase slowly, the organ indexs such as spleen, thymus gland
Reduction, digestive system form and exception of function etc. are also the main performance of syndrome of spleen deficiency.Intemperance of taking food model of spleen deficiency modeling process
In, mouse gradually appears the symptoms such as semiliquid stool, burnout are moved less, chilly flocks together, coat is fluffy/withered, and body weight increase is slow, spleen weight amount
Substantially reduced, spleen index is obviously reduced, and alvine pushing rate is substantially reduced；Apositia model mice body weight increase is slow, alvine pushing rate
It is substantially reduced, shows two kinds of model modeling successes.Alcohol reflux extract group mouse weight, thymus gland, spleen weight dramatically increase (P <
0.01), thymus index, index and spleen index increase (P < 0.05)；Water decoction-alcohol sedimentation extract group mouse weight, thymic weight increase (P
<0.05)；Only intemperance of taking food model of spleen deficiency the weight of animals has certain influence (P < 0.05) to water decoction extract group mouse；Each extract
Group can significantly improve model mice alvine pushing rate, and three's effect is suitable.
Motilin (MTL) is a kind of braingut petide hormone, and physiological action is mainly the power for influencing stomach and enteron aisle, induces stomach
Strong contraction and the apparent segmentation movement of small intestine reinforce the movement of ileum and colon, play cleaning to gastrointestinal cavity content.It can also
The secretion for stimulating pepsin, pancreatic juice, facilitates food digestion.MTL content can largely reflect gastrointestinal tract in blood
Movement and functional status.Ghrelin be scientist's discovery in recent years it is a kind of the endocrine growth hormone cinogenic agent of stomach by
The endogenic ligand of body, it can whet the appetite, and adjust energy balance, and concentration increase can cause foraging behavior.Apositia model
Mouse Ghrelin level and model of spleen deficiency mouse MTL level are significantly lower than Normal group.Alcohol reflux extract group, decocting
Alcohol precipitation extract group mice serum MTL, Ghrelin content obviously increases (P < 0.01), and water decoction extract group has no significant effect, table
Bright fevervine refluxing extraction object, water decoction-alcohol sedimentation extract can improve animal functions of intestines and stomach, promote appetite.
Compared with prior art, the study result show that, fevervine extract is dynamic to apositia model and spleen deficiency
Object model has apparent therapeutic effect, shows as the various spleen deficiency symptoms for being obviously improved model of spleen deficiency mouse, promotes spleen, thymus gland
The increase of equal organ weights；Mice with spleen deficiency, apositia mouse weight is promoted to increase, and the alimentary canal of animal pattern can be obviously improved
Function.Wherein, the drug effect of the extract for treating infantile malnutrition of fevervine Alcohol refluxing method preparation is the most significant, is optimum extraction work
Skill.
In conclusion preparation method simple and stable of the present invention, preparation cost is low, and preparation efficiency is high；The fevervine of preparation mentions
It takes object treatment infantile malnutrition effect good, chicken has been pushed to lose boisiana extract in the development of clinical application and medicine preparation.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated, but is not intended as the foundation limited the present invention.
Embodiment 1.One breeder loses the preparation method of boisiana extract, and this method is to take fevervine medicinal material, adds 8 times of 70% second of amount
Alcohol, refluxing extraction 3 times, first time 1.5h, remaining each 1h every time, filtration recycles ethyl alcohol and is concentrated into no alcohol taste.
Embodiment 2.One breeder loses the preparation method of boisiana extract, and this method is to take fevervine medicinal material, adds 6 times of 70% second of amount
Alcohol, refluxing extraction 2 times, first time 1h, second of 1h, filtration recycles ethyl alcohol and is concentrated into no alcohol taste.
Embodiment 3.One breeder loses the preparation method of boisiana extract, and this method is to take fevervine medicinal material, adds 7 times of 70% second of amount
Alcohol, refluxing extraction 3 times, first time 2h, remaining each 1h every time, filtration recycles ethyl alcohol and is concentrated into no alcohol taste.
4 one breeder of embodiment loses the preparation method of boisiana extract, and this method is to take fevervine medicinal material, adds 9 times of 70% second of amount
Alcohol, refluxing extraction 4 times, first time 2h, remaining each 1h every time, filtration recycles ethyl alcohol and is concentrated into no alcohol taste.
Claims (4)
1. the preparation method of breeder mistake boisiana extract, it is characterised in that: this method is to return 70% ethyl alcohol of fevervine medicinal material
Stream extracts, and recycles ethyl alcohol and is concentrated into no alcohol taste.
2. the preparation method that a breeder according to claim 1 loses boisiana extract, it is characterised in that: the method is to take chicken
It swears rattan medicinal material, adds 6-10 times to measure 70% ethyl alcohol, refluxing extraction 2-5 times, first time 1-3h, remaining each 1h every time, filtration, recycling second
Alcohol is simultaneously concentrated into no alcohol taste.
3. the preparation method that a breeder according to claim 2 loses boisiana extract, it is characterised in that: the method is to take chicken
It swears rattan medicinal material, adds 7-9 times to measure 70% ethyl alcohol, refluxing extraction 2-4 times, first time 1-2h, remaining each 1h every time, filtration, recycling second
Alcohol is simultaneously concentrated into no alcohol taste.
4. the preparation method that a breeder according to claim 3 loses boisiana extract, it is characterised in that: the method is to take chicken
It swears rattan medicinal material, adds 8 times of 70% ethyl alcohol of amount, refluxing extraction 3 times, first time 1.5h, remaining each 1h every time, filtration, recycling ethyl alcohol is simultaneously
It is concentrated into no alcohol taste.
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2019
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| CN101991592A (en) * | 2009-08-14 | 2011-03-30 | 上海颐华生物医药有限公司 | Application of paederoside to preparation of analgesic and/or anti-inflammatory medicaments |
| CN104474068A (en) * | 2014-11-06 | 2015-04-01 | 宁波大昌药业有限公司 | Paederia scandens extractive and application thereof |
| CN105381388A (en) * | 2015-12-21 | 2016-03-09 | 李建敏 | Traditional Chinese medicine composition for treating stomach discomfort and preparing method thereof |
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| Title |
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| 王绍军等: "鸡矢藤提取物对糖尿病模型小鼠的保护作用", 《中国实验方剂学杂志》 * |
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