CN110178849B - Application of psoralen in preventing and treating plant bacterial diseases - Google Patents
Application of psoralen in preventing and treating plant bacterial diseases Download PDFInfo
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- CN110178849B CN110178849B CN201910436654.1A CN201910436654A CN110178849B CN 110178849 B CN110178849 B CN 110178849B CN 201910436654 A CN201910436654 A CN 201910436654A CN 110178849 B CN110178849 B CN 110178849B
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- psoralen
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- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 title claims abstract description 62
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 208000035143 Bacterial infection Diseases 0.000 title claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims abstract description 18
- 241000196324 Embryophyta Species 0.000 claims abstract description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 14
- 235000009566 rice Nutrition 0.000 claims abstract description 14
- 235000013311 vegetables Nutrition 0.000 claims abstract description 7
- 241000589634 Xanthomonas Species 0.000 claims abstract description 6
- 241000209094 Oryza Species 0.000 claims description 13
- 241001272684 Xanthomonas campestris pv. oryzae Species 0.000 claims description 5
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 8
- 239000003899 bactericide agent Substances 0.000 abstract description 5
- 150000001875 compounds Chemical class 0.000 abstract description 4
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000375 suspending agent Substances 0.000 description 4
- 241000227653 Lycopersicon Species 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 244000000005 bacterial plant pathogen Species 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000004562 water dispersible granule Substances 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000005762 Dimoxystrobin Substances 0.000 description 1
- 241000228457 Leptosphaeria maculans Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589626 Pseudomonas syringae pv. tomato Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000589649 Xanthomonas campestris pv. campestris Species 0.000 description 1
- 241000626572 Xanthomonas oryzae pv. oryzicola Species 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- CWVRPJSBNHNJSI-XQNSMLJCSA-N coumoxystrobin Chemical compound C1=C2OC(=O)C(CCCC)=C(C)C2=CC=C1OCC1=CC=CC=C1\C(=C/OC)C(=O)OC CWVRPJSBNHNJSI-XQNSMLJCSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- WXUZAHCNPWONDH-DYTRJAOYSA-N dimoxystrobin Chemical compound CNC(=O)C(=N\OC)\C1=CC=CC=C1COC1=CC(C)=CC=C1C WXUZAHCNPWONDH-DYTRJAOYSA-N 0.000 description 1
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- OXWFFWJKUNMMSO-UHFFFAOYSA-N n-octyl-n'-[2-(octylamino)ethyl]ethane-1,2-diamine Chemical compound CCCCCCCCNCCNCCNCCCCCCCC OXWFFWJKUNMMSO-UHFFFAOYSA-N 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
An application of psoralen in preventing and treating plant bacterial diseases belongs to the technical field of plant-derived bactericides and discloses an application of psoralen in preventing and treating plant bacterial diseases. The compound can effectively inhibit the growth of xanthomonas species such as rice bacterial streak germ, cruciferous vegetable black rot germ and the like, and the plant source bactericide prepared by processing the compound as an active ingredient can be used for preventing and treating plant bacterial diseases caused by the xanthomonas species. The compound can be used alternately with other bactericides to delay the generation of drug resistance.
Description
Technical Field
The invention belongs to the technical field of plant-derived bactericides, and particularly relates to application of psoralen in preventing and treating plant bacterial diseases.
Background
Plant bacteriosis is an important plant disease, and once the disease occurs, the disease causes great loss to agricultural production. At present, the prevention and control medicament for plant bacterial diseases is seriously deficient, and the common prevention and control medicament has the problem of drug resistance after long-term use, so that the development of a novel prevention and control medicament for alternative use is urgently needed.
The psoralen is derived from fructus Psoraleae (fructus Psoraleae) of LeguminosaePsoralea corylifoliaL.) furocoumarins compound of fruit, has abundant bioactivity. In the available literature and patents, "the use of psoralen as an agricultural bactericide or insecticide" (patent application No. 201110050088.4) discloses the use of psoralen for preventing and treating plant fungal diseases, "a bactericidal composition containing psoralen and Xinjunan" (patent application No. 201810340235.3), "a bactericidal composition containing psoralen and dimoxystrobin" (patent application No. 201810340236.8) and "a bactericidal composition containing psoralen and coumoxystrobin" (patent application No. 201810340242.3) also disclose that the composition containing psoralen can be used for preventing and treating plant fungal diseases, but no report that psoralen is used for preventing and treating plant bacterial diseases is available at present.
Disclosure of Invention
In view of the above disadvantages, the present invention provides the use of psoralen in the prevention and treatment of plant bacterial diseases.
The invention is realized by the following technical scheme.
The psoralen is obtained by separating and purifying fructus Psoraleae (purity is more than 98%), and can be used as active ingredient and pesticide adjuvant to make into any dosage form allowable for pesticide.
The dosage forms comprise powder, wettable powder, soluble powder, granules, aqueous solution, missible oil, aqueous emulsion, microemulsion, water dispersible granules, suspending agent, smoke agent, sustained release agent, seed coating agent or oil agent.
The application refers to the prevention and treatment of bacterial diseases caused by xanthomonas such as rice bacterial streak germ, rice bacterial streak germ and cruciferous vegetable black rot germ.
The invention proves that psoralen has significant bacteriostatic activity on xanthomonas such as rice bacterial streak germ, rice bacterial streak germ and cruciferous vegetable black rot germ through research, and can be developed into a control medicament for alternate use, thereby relieving the generation of drug resistance.
Description of the drawings:
FIG. 1 chemical structural formula of psoralen
Detailed Description
The following examples further illustrate the present invention in detail, but the present invention is not limited to the contents contained in the following examples.
Example 1:
psoralen is weighed and mixed with auxiliary materials such as lignin, xanthan gum, glycol, organic silicone defoamer, sodium benzoate and the like to prepare the suspending agent with the effective component concentration of 20% according to the conventional preparation process.
Example 2:
psoralen is weighed and mixed with routine auxiliary materials such as diatomite, sodium dodecyl benzene sulfonate, lignin and the like to prepare wettable powder with the concentration of effective components of 25 percent according to a routine preparation process.
Example 3:
psoralen is weighed and prepared into water dispersible granules with the effective component concentration of 50% with conventional auxiliary materials such as kaolin, sodium lignin sulfonate and the like according to a conventional preparation process.
Example 4:
determination of psoralen Activity against plant pathogenic bacteria:
the assay object comprises rice bacterial blight bacterium (Xanthomonas oryzae pv. oryzae) Bacterial leaf streak disease of rice (1)Xanthomonas oryzae pv. oryzicola）、Leaf mold of cruciferous vegetables (A. fructicola (B.))Xanthomonas campestris pv. campestris) And bacterial leaf spot of tomato (C)Pseudomonas syringae pv. tomato）。
Psoralen is dissolved in DMSO to prepare a mother solution with the concentration of 10000 mug/mL, and gradient dilution is carried out by using DMSO for standby. Adding psoralen solution or DMSO with equal volume into NB culture solution to obtain drug-containing culture solution, and pre-culturing to logarithmic phase with spectrophotometer to obtain bacterial culture OD600The value was adjusted to 0.02 and added to the drug-containing medium at a ratio of 1:200, with 3 replicates per treatment. The control was grown to logarithmic growth phase by shaking at 175rpm at 28 ℃ and OD was measured with a spectrophotometer600And the inhibition rate was calculated. Inhibition (%) = (control OD)600value-Add medicine and handle OD600Value)/control OD600The value is 100. Calculating the EC of psoralen according to the inhibition rate of different concentrations50The value is obtained.
And (3) test results: psoralen activity against plant pathogenic bacteria is shown in table 1. As can be seen from the results in the table, psoralen has good bacteriostatic activity on xanthomonas such as rice bacterial blight germ, rice bacterial streak germ and cruciferous vegetable black rot germ, but has no activity on pseudomonas such as tomato bacterial spot germ in a tested range.
TABLE 1 bacteriostatic Activity of psoralen against plant pathogenic bacteria
| Pathogenic bacteria | EC50（μg/mL） |
| Bacterial blight of rice | 4.67±0.61 |
| Bacterial leaf streak germ of rice | 8.73±2.17 |
| Leptosphaeria maculans of cruciferous vegetables | 9.41±0.39 |
| Bacterial leaf spot of tomato | > 50 |
Example 5:
the control effect of the 20% psoralen suspension prepared according to example 1 on bacterial blight of rice was as follows:
diluting 20% psoralen suspending agent at 1000 times, 500 times and 250 times, and spraying to conventional greenhouse-planted 'IR 24' rice with clear water as blank control. The bacterial suspension of pathogenic bacteria is used for artificial inoculation one day before the medicine is taken, the medicine is sprayed for the second time 7 days after the medicine is taken, and after the control is fully developed, the disease condition is investigated and the control effect is calculated.
The test result shows that: under the treatment of the test agent, the medicament with each concentration has no adverse effect on crops and grows normally. The suspending agent prepared according to the example 1 has good control effect on the bacterial blight of rice. The 250 times of diluent has the control effect on the bacterial leaf blight of rice more than 60 percent, and the 1000 times of diluent has the control effect on the bacterial leaf blight of rice more than 30 percent.
Claims (2)
1. The application of psoralen in preventing and treating plant bacterial diseases caused by xanthomonas is provided.
2. Use according to claim 1, characterized in that the psoralen is useful for inhibiting the growth of bacterial blight of rice, bacterial leaf spot of rice and black rot of cruciferous vegetables.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910436654.1A CN110178849B (en) | 2019-05-24 | 2019-05-24 | Application of psoralen in preventing and treating plant bacterial diseases |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910436654.1A CN110178849B (en) | 2019-05-24 | 2019-05-24 | Application of psoralen in preventing and treating plant bacterial diseases |
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| Publication Number | Publication Date |
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| CN110178849A CN110178849A (en) | 2019-08-30 |
| CN110178849B true CN110178849B (en) | 2021-03-23 |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843127A (en) * | 2006-05-16 | 2006-10-11 | 沈阳化工学院 | Plant source bacteriocide for agriculture |
| CN101219932A (en) * | 2008-01-25 | 2008-07-16 | 沈阳化工学院 | Bakuchiol for preventing and controlling plant disease |
| CN102067850A (en) * | 2011-03-02 | 2011-05-25 | 江苏省中国科学院植物研究所 | Application of psoralen as agricultural disinfectant or pesticide |
| CN107743970A (en) * | 2017-12-06 | 2018-03-02 | 惠州市支持网知识产权运营有限公司 | Bactericidal composition containing psoralen and gold alloy hydride |
| CN107950550A (en) * | 2017-12-11 | 2018-04-24 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of bactericidal composition |
| CN108477187A (en) * | 2018-04-16 | 2018-09-04 | 杜桂香 | A kind of bactericidal composition containing psoralen and coumoxystrobin |
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-
2019
- 2019-05-24 CN CN201910436654.1A patent/CN110178849B/en active Active
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