CN110178849A - Purposes of the psoralen in prevention and treatment phytobacterial disease - Google Patents
Purposes of the psoralen in prevention and treatment phytobacterial disease Download PDFInfo
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- CN110178849A CN110178849A CN201910436654.1A CN201910436654A CN110178849A CN 110178849 A CN110178849 A CN 110178849A CN 201910436654 A CN201910436654 A CN 201910436654A CN 110178849 A CN110178849 A CN 110178849A
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- Prior art keywords
- psoralen
- purposes
- prevention
- treatment
- phytobacterial
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
Abstract
Purposes of the psoralen in prevention and treatment phytobacterial disease, belongs to botanical fungicide technical field, discloses purposes of the psoralen in prevention and treatment phytobacterial disease.The compound can effectively inhibit the growth of the Xanthomonas campestris such as rice leaf spot bacteria, xanthomonas oryzae pv. oryzicola and brassicaceous vegetable black rot, which can be used for phytobacterial disease caused by preventing and treating Xanthomonas campestris as the botanical fungicide that active constituent is processed into.The generation that can delay drug resistance is used alternatingly using the compound and other fungicide.
Description
Technical field
The invention belongs to botanical fungicide technical fields, and in particular to psoralen is in prevention and treatment phytobacterial disease
Purposes.
Background technique
Phytobacterial disease is a kind of important plant disease, once morbidity can cause huge loss to agricultural production.
Phytobacterial disease pesticide control is seriously deficient at present, and common pesticide control is due to being used for a long time existing resistance problems,
Therefore it is badly in need of developing novel pesticide control to be used alternatingly.
Psoralen be derived from legumes psoraleae (Psoralea corylifoliaL. a kind of) furans of fruit
Coumarin kind compound has bioactivity abundant.In the document and patent that can be retrieved at present, " psoralen is as agriculture
The purposes of industry fungicide or insecticide " (number of patent application: 201110050088.4) discloses psoralen prevention and treatment plant epiphyte
The purposes of disease, " bactericidal composition containing psoralen and octyl trichlamide " (number of patent application: 201810340235.3), " contain
Have the bactericidal composition of psoralen and dimoxystrobin " (number of patent application: 201810340236.8) and " one kind contains psoralen
With the bactericidal composition of coumoxystrobin " (number of patent application: 201810340242.3) etc. multiple patents are also disclosed containing Psoralen
The composition of rouge element can be used for preventing and treating fungal diseases of plants, but there has been no psoralens for preventing and treating phytobacterial disease at present
Report.
Summary of the invention
Against the above deficiency, the purposes the present invention provides psoralen in prevention and treatment phytobacterial disease.
The present invention is achieved by the following technical solutions.
The psoralen is (purity > 98%) isolated and purified from psoralea corylifolia fruit, can be used as active constituent
Any dosage form of pesticide permission is processed into insecticides adjuvant.
The dosage form includes pulvis, wettable powder, soluble powder, granula, aqua, missible oil, aqueous emulsion, micro emulsion
Agent, water dispersible granules, suspending agent, fumicants, sustained release agent, seed coat agent or finish.
The purposes, which refers to, can be used for preventing and treating rice leaf spot bacteria, xanthomonas oryzae pv. oryzicola and Cruciferae vegetable
Bacteriosis caused by the Xanthomonas campestris such as dish black rot.
The present invention has shown that psoralen to rice leaf spot bacteria, xanthomonas oryzae pv. oryzicola and cruciate flower by research
The Xanthomonas campestris such as section black rot of cabbage bacterium have significant bacteriostatic activity, can be developed into pesticide control and are used alternatingly, thus
Alleviate the generation of drug resistance.
Detailed description of the invention:
The chemical structural formula of Fig. 1 psoralen
Specific embodiment
The specific embodiment of the invention is described in further detail below, but the contents of the present invention be not restricted to it is following
The content that embodiment includes.
Embodiment 1:
Psoralen is weighed, with the auxiliary materials such as lignin, xanthan gum, ethylene glycol, organosilicon ketone defoaming agent, sodium benzoate according to normal
The suspending agent that effective constituent concentration is 20% is made in rule preparation process.
Embodiment 2:
Psoralen is weighed, with the customary adjuvants such as diatomite, neopelex, lignin according to conventional fabrication process system
The wettable powder for being 25% at effective component concentration.
Embodiment 3:
Psoralen is weighed, effective component is made according to conventional fabrication process with customary adjuvants such as kaolin, sodium lignin sulfonates
The water dispersible granules that concentration is 50%.
Embodiment 4:
Psoralen is to the active measurement of plant pathogenetic bacteria:
Measure object include rice leaf spot bacteria (Xanthomonas oryzae pv. oryzae), paddy bacterial streak
Germ (Xanthomonas oryzae pv. oryzicola), brassicaceous vegetable black rot (Xanthomonas campestris pv. campestris) and bacterial spot of tomato bacterium (Pseudomonas syringae pv.tomato).
Psoralen is dissolved in DMSO, is configured to the mother liquor that concentration is 10000 μ g/mL, and dilute using DMSO progress gradient
It is stand-by after releasing.Each concentration psoralen solution or isometric DMSO are separately added into NB culture solution, drug containing culture solution is made, used
Spectrophotometer is by the bacterial cultures OD of preculture to logarithmic growth phase600Value is adjusted to 0.02, and is added in the ratio of 1:200
In drug containing culture solution, 3 repetitions of each processing.28 DEG C of 175rpm, which shake training, keeps control long to logarithmic growth phase, uses spectrophotometer
Measure OD600And calculate inhibiting rate.Inhibiting rate (%)=(control OD600Value ﹣ agent-feeding treatment OD600Value)/control OD600Value × 100.Root
The EC of psoralen is calculated according to the inhibiting rate of various concentration50Value.
Test result: psoralen is as shown in table 1 to plant pathogenetic bacteria activity.As can be seen from the results in the table that Psoralen
Rouge element to the Xanthomonas campestris such as rice leaf spot bacteria, xanthomonas oryzae pv. oryzicola and brassicaceous vegetable black rot have compared with
Good bacteriostatic activity, but it is inactive in tested range to pseudomonads such as bacterial spot of tomato bacterium.
Bacteriostatic activity of 1 psoralen of table to plant pathogenetic bacteria
| Pathogen | EC50(μ g/mL) |
| Rice leaf spot bacteria | 4.67±0.61 |
| Xanthomonas oryzae pv. oryzicola | 8.73±2.17 |
| Brassicaceous vegetable black rot | 9.41±0.39 |
| Bacterial spot of tomato bacterium | > 50 |
Embodiment 5:
The 20% psoralen suspending agent prepared by embodiment 1 is to the control efficiency of bacterial blight of rice:
It is sprayed on by 1000 times, 500 times and 250 times 20% psoralen suspending agents of dilution proportion using clear water as blank control
' IR24 ' rice of greenhouse general planting.Artificial infection is carried out using the bacteria suspension of pathogenetic bacteria on the day before medication, 7 after medication
It carries out second and sprays, and after the onset of compareing sufficiently, investigates incidence and calculates preventive effect.
Test result shows: in the case where handling for amount of reagent, each drug concentrations have no adverse effects to crop, and growth is normal.
The suspending agent prepared by embodiment 1 is good to bacterial blight of rice control efficiency.250 times of dilutions prevent bacterial blight of rice
Control 60% or more effect, and 1000 times of dilutions to the preventive effect of bacterial blight of rice 30% or more.
Claims (4)
1. purposes of the psoralen in prevention and treatment phytobacterial disease.
2. purposes described in accordance with the claim 1, it is characterised in that psoralen is as active constituent and insecticides adjuvant processing system
Any dosage form allowed at pesticide.
3. dosage form according to claim 2, it is characterised in that the dosage form includes pulvis, wettable powder, solubility
Pulvis, granula, aqua, missible oil, aqueous emulsion, microemulsion, water dispersible granules, suspending agent, fumicants, sustained release agent, seed coat agent or finish.
4. purposes described in accordance with the claim 1, it is characterised in that the psoralen can be used for inhibiting bacterial blight of rice
The growth of the Xanthomonas campestris such as bacterium, xanthomonas oryzae pv. oryzicola and brassicaceous vegetable black rot；The psoralen is made
It can be used for phytobacterial disease caused by preventing and treating Xanthomonas campestris for the botanical fungicide that active constituent is processed into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910436654.1A CN110178849B (en) | 2019-05-24 | 2019-05-24 | Application of psoralen in preventing and treating plant bacterial diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910436654.1A CN110178849B (en) | 2019-05-24 | 2019-05-24 | Application of psoralen in preventing and treating plant bacterial diseases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110178849A true CN110178849A (en) | 2019-08-30 |
| CN110178849B CN110178849B (en) | 2021-03-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910436654.1A Active CN110178849B (en) | 2019-05-24 | 2019-05-24 | Application of psoralen in preventing and treating plant bacterial diseases |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843127A (en) * | 2006-05-16 | 2006-10-11 | 沈阳化工学院 | Plant source bacteriocide for agriculture |
| CN101219932A (en) * | 2008-01-25 | 2008-07-16 | 沈阳化工学院 | Bakuchiol for preventing and controlling plant disease |
| CN102067850A (en) * | 2011-03-02 | 2011-05-25 | 江苏省中国科学院植物研究所 | Application of psoralen as agricultural disinfectant or pesticide |
| CN107743970A (en) * | 2017-12-06 | 2018-03-02 | 惠州市支持网知识产权运营有限公司 | Bactericidal composition containing psoralen and gold alloy hydride |
| CN107950550A (en) * | 2017-12-11 | 2018-04-24 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of bactericidal composition |
| CN108477187A (en) * | 2018-04-16 | 2018-09-04 | 杜桂香 | A kind of bactericidal composition containing psoralen and coumoxystrobin |
| CN108633893A (en) * | 2018-04-16 | 2018-10-12 | 张友兰 | Bactericidal composition containing psoralen and dimoxystrobin |
| CN108633892A (en) * | 2018-04-16 | 2018-10-12 | 张月婵 | Bactericidal composition containing psoralen and octyl trichlamide |
| CN108633894A (en) * | 2018-04-16 | 2018-10-12 | 赖振明 | A kind of bactericidal composition containing psoralen and Nuo Wo mycins |
| CN109303074A (en) * | 2018-11-06 | 2019-02-05 | 于海峰 | The mix preparation of controlling plant diseases and its application |
-
2019
- 2019-05-24 CN CN201910436654.1A patent/CN110178849B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843127A (en) * | 2006-05-16 | 2006-10-11 | 沈阳化工学院 | Plant source bacteriocide for agriculture |
| CN101219932A (en) * | 2008-01-25 | 2008-07-16 | 沈阳化工学院 | Bakuchiol for preventing and controlling plant disease |
| CN102067850A (en) * | 2011-03-02 | 2011-05-25 | 江苏省中国科学院植物研究所 | Application of psoralen as agricultural disinfectant or pesticide |
| CN107743970A (en) * | 2017-12-06 | 2018-03-02 | 惠州市支持网知识产权运营有限公司 | Bactericidal composition containing psoralen and gold alloy hydride |
| CN107950550A (en) * | 2017-12-11 | 2018-04-24 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of bactericidal composition |
| CN108477187A (en) * | 2018-04-16 | 2018-09-04 | 杜桂香 | A kind of bactericidal composition containing psoralen and coumoxystrobin |
| CN108633893A (en) * | 2018-04-16 | 2018-10-12 | 张友兰 | Bactericidal composition containing psoralen and dimoxystrobin |
| CN108633892A (en) * | 2018-04-16 | 2018-10-12 | 张月婵 | Bactericidal composition containing psoralen and octyl trichlamide |
| CN108633894A (en) * | 2018-04-16 | 2018-10-12 | 赖振明 | A kind of bactericidal composition containing psoralen and Nuo Wo mycins |
| CN109303074A (en) * | 2018-11-06 | 2019-02-05 | 于海峰 | The mix preparation of controlling plant diseases and its application |
Non-Patent Citations (2)
| Title |
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| 关丽杰: "《补骨脂抑菌活性成分及其制剂研究》", 《沈阳农业大学》 * |
| 胡丽丽等: "无花果叶中抑菌活性成分―补骨脂素的研究 ", 《青岛农业大学学报(自然科学版)》 * |
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| CN110178849B (en) | 2021-03-23 |
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