CN113598186A - Bactericidal composition and application thereof - Google Patents
Bactericidal composition and application thereof Download PDFInfo
- Publication number
- CN113598186A CN113598186A CN202111087102.8A CN202111087102A CN113598186A CN 113598186 A CN113598186 A CN 113598186A CN 202111087102 A CN202111087102 A CN 202111087102A CN 113598186 A CN113598186 A CN 113598186A
- Authority
- CN
- China
- Prior art keywords
- meptyldinocap
- bactericidal composition
- amine
- late blight
- triflumizole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 75
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 51
- NIOPZPCMRQGZCE-WEVVVXLNSA-N 2,4-dinitro-6-(octan-2-yl)phenyl (E)-but-2-enoate Chemical compound CCCCCCC(C)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1OC(=O)\C=C\C NIOPZPCMRQGZCE-WEVVVXLNSA-N 0.000 claims abstract description 50
- 239000005806 Meptyldinocap Substances 0.000 claims abstract description 50
- 239000003094 microcapsule Substances 0.000 claims abstract description 25
- -1 triflumizole amine Chemical class 0.000 claims abstract description 22
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- 239000003899 bactericide agent Substances 0.000 description 4
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- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 2
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- ZVXKYWHJBYIYNI-UHFFFAOYSA-N 1h-pyrazole-4-carboxamide Chemical class NC(=O)C=1C=NNC=1 ZVXKYWHJBYIYNI-UHFFFAOYSA-N 0.000 description 1
- XCGBHLLWJZOLEM-UHFFFAOYSA-N 3-(difluoromethyl)-N-(7-fluoro-1,1,3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1-methyl-1H-pyrazole-4-carboxamide Chemical compound CC1CC(C)(C)C(C(=CC=2)F)=C1C=2NC(=O)C1=CN(C)N=C1C(F)F XCGBHLLWJZOLEM-UHFFFAOYSA-N 0.000 description 1
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- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
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- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
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- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
- A01N25/28—Microcapsules or nanocapsules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of pesticides, and particularly relates to a bactericidal composition and application thereof. The sterilization composition comprises the effective components of the triflumizole amine and the meptyldinocap, wherein the mass ratio of the triflumizole amine to the meptyldinocap is (6-10) to (13-17). The invention also discloses application of the bactericidal composition containing the triflumizole amine and the meptyldinocap in preventing and treating diseases in crops. According to the invention, the triflumizole amine and the meptyldinocap are compounded to generate a synergistic effect, and the two are prepared into the microcapsule suspending agent taking the melamine formaldehyde resin as an encapsulating material, so that the formed bactericidal composition has a good bactericidal effect, and particularly has a good prevention and treatment effect on the cherry late blight. Compared with single agents of the triflumizole amine and the meptyldinocap, the bactericidal composition is low in dosage and cost, and meanwhile, the side effect of the bactericidal composition is low, and the safety, the yield and the like of crops cannot be affected.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a bactericidal composition and application thereof.
Background
In recent years, low grade flagellate fungal diseases represented by late blight have been developed in large scale, resulting in serious deterioration in crop quality and yield. The cherry tomato late blight is a disease caused by Phytophthora infestans (Phytophthora infestans) and occurring in cherry tomatoes. Occurring throughout the growing period of the fruit, the adult stage is more susceptible, the leaves, stems and fruit may be damaged, and the leaves and green fruit are most severely damaged. The infected leaves are mostly the lower parts, dark green irregular water immersion lesions appear at the edges or the leaf tips of the leaves, the lesions are enlarged and browned, the areas of the lesions are large, and the outlines of the lesions are not obvious. Finally, the entire diseased leaf is hung on the petiole. Sparse white mold can be seen at the leaf back disease and junction under high humidity; the pathogens can extend to the stem, the disease on the stem becomes longer, dark brown, slightly concave, or shrinks, and the edges sometimes become sparse. Mildew, which is easily caused by the disease of the diseased part, fruit stem and fruit stem, is the same as that of the stem. Lesions on green fruits are initially oily and dark green and then turn dark brown to brown. The pathological changes are irregular cloud-shaped, the diseased fruits are hard and uneven, the fruits generally do not fall off, and the upper part is long when the humidity is high. A small amount of white mold, quickly rotten. Seedlings may also become infected. In severe cases, the entire plant will wither or collapse.
At present, the activity of the mainstream efficient bactericide such as pyraclostrobin is poor, and metalaxyl-M and the like generate more serious resistance due to longer service time. Therefore, selecting effective chemical components with high activity on diseases, combining different medicaments by applying the prior art, and then preventing and treating the diseases is an effective way for exploring and developing a new plant protection technology. However, the effect of a combination of different agents is not certain, and it may produce a synergistic effect or an antagonistic effect. And the effect of combinations of different agents on different diseases is not determined. Therefore, the field still searches for a bactericide which can effectively kill pathogenic bacteria and prevent and control the cherry tomato late blight.
The pesticide microcapsule suspending agent is a pesticide formulation which is prepared by forming a micro container with a core-shell structure by using a synthetic or natural polymer material, coating a pesticide in the micro container and suspending the pesticide in water. The microcapsule suspending agent has the characteristics of being beneficial to the chemical stability of the effective ingredients of the pesticide. The choice of the encapsulating material is a key material for the shell formation of the microcapsule suspension, and is determined to play a role in avoiding the rapid volatilization and escape of the more volatile medicament. Avoiding the damage of the photosensitive medicament by light and slowing down the hydrolysis speed of the medicament easy to hydrolyze. Greatly reduces the poisoning accidents caused by pesticides with higher toxicity, and has extremely high safety and environmental protection. The melamine formaldehyde resin is a polymer obtained by the reaction of melamine and formaldehyde, is stable at high temperature, and has self-extinguishing property, arc resistance and good mechanical property. At present, the microcapsule suspension is mainly applied to the construction, and the microcapsule suspension is not applied to a capsule material and has stable performance.
Disclosure of Invention
In view of the above, the technical problem to be solved by the present invention is to provide a bactericidal composition for effectively preventing and treating late blight and its application.
The sterilization composition provided by the invention consists of the triflumizole amine and the meptyldinocap.
The thiabendazole amine is used for preventing and treating high-grade fungi such as basidiomycetes in the prior art, and the meptyldinocap is used for preventing and treating high-grade fungi such as ascomycetes in the prior art. According to the sterilization composition, the triflumizole amine and the meptyldinocap are compounded, so that the formed sterilization composition generates a synergistic effect, not only can have a good sterilization effect, but also can be used for preventing and treating phytophthora. Meanwhile, the bactericidal composition has low side effect and cannot influence the safety, the yield and the like of crops.
In the invention, the mass ratio of the triflumizole amine to the meptyldinocap is (2-32) to (60-1). In the embodiment of the invention, the mass ratio of the fluoroandenazole bacteria amine to the meptyldinocap is (6-10) to (13-17).
Researches show that the penflufen and the meptyldinocap can obtain good synergistic synergy within the mass ratio of (6-10) to (13-17), and the synergistic synergy coefficient SR is 1.50-5.54.
The mass ratio of the fluoroandenazole amine to the meptyldinocap is (8-10): (13-15), so that a better synergistic effect can be obtained, and the synergistic coefficient SR is 3.87-5.54.
In the embodiment of the invention, the mass ratio of the triflumizole amine to the meptyldinocap is 6:17, 7:16, 8:15, 9:14 or 10: 13. Wherein, when the ratio of the thiabendazole: when the meptyldinocap is 8:15, the synergistic effect is highest, the synergistic coefficient is 5.54, and LC50Is 0.7526mg/L, LC90It was 3.7501 mg/L.
The bactericidal composition provided by the invention shows a good phytophthora resistance effect in an indoor bioassay test, and shows a good late blight resistance effect in a field experiment.
The bactericidal composition disclosed by the invention is applied to inhibition of phytophthora.
Phytophthora is a non-fungal plant pathogen belonging to the species of oophyta, and in the present invention, the fungicidal composition has an inhibitory effect on Phytophthora. Specifically, phytophthora which may be inhibited by the bactericidal composition of the present invention includes phytophthora capsici, phytophthora rubberans, phytophthora clusteri, phytophthora mellonella, phytophthora infestans, phytophthora cinnamomi, phytophthora ramie, phytophthora citri reticulata, phytophthora nicotianae, phytophthora palmae, phytophthora tarda, phytophthora infestans, phytophthora citrulli, phytophthora cyperi, phytophthora pratensis, phytophthora infestans, phytophthora humicola, phytophthora megalosa, rice field phytophthora infestans, phytophthora calla, phytophthora camphora, phytophthora drechslendersonii, phytophthora cactoruloides, phytophthora cryptheca and phytophthora cowpea.
In the embodiment of the invention, the antibacterial effect of the bactericidal composition is researched by taking Phytophthora infestans (Phytophthora infestans) as an experimental object. Therefore, the invention provides the application of the antibacterial composition in inhibiting phytophthora infestans.
The inhibition of the invention comprises: inhibiting mycelium formation, destroying mycelium structure, inhibiting phytophthora proliferation and/or killing phytophthora. The inhibiting proliferation comprises: killing oospores, inhibiting oospore formation, inhibiting sporangial formation, inhibiting spore release, and/or killing spores.
The bactericidal composition disclosed by the invention is applied to prevention and treatment of late blight of crops.
Late blight, also known as spot blight and leaf blight, occurs commonly in vegetables. The bactericidal composition can prevent and treat late blight caused by phytophthora. Specifically, the late blight possibly having the prevention and treatment effect of the bactericidal composition comprises the following components: tomato late blight, cherry tomato late blight, potato late blight, grape late blight and/or pepper late blight.
In the embodiment of the invention, the control effect of the bactericidal composition bought from phenol is researched by taking cherry tomatoes with late blight as experimental subjects. Therefore, the invention provides the application of the bactericidal composition in preventing and treating the cherry tomato late blight.
The prevention and treatment in the present invention includes prevention and/or treatment. The prevention comprises the step of applying a medicament containing the bactericidal composition to prevent late blight from occurring before the plant diseases occur. The treatment comprises applying the medicine containing the bactericidal composition after the plant is attacked so as to inhibit the development of the late blight and/or eliminate the late blight.
The invention also provides a medicine for preventing and treating late blight, which comprises the bactericidal composition and auxiliary materials acceptable in pesticides.
In the medicine provided by the invention, the mass fraction of the triflumizole amine is 2-32%, and the mass fraction of the meptyldinocap is 1-60%.
In some embodiments, the mass fraction of the bactericidal composition in the medicine for preventing and treating late blight is 19-27%.
Specifically, the mass fraction of the triflumizole amine is 6%, and the mass fraction of the meptyldinocap is 17%. Or the mass fraction of the triflumizole amine is 7 percent, and the mass fraction of the meptyldinocap is 16 percent. Or the mass fraction of the triflumizole amine is 8 percent, and the mass fraction of the meptyldinocap is 15 percent. Or the mass fraction of the triflumizole amine is 9 percent, and the mass fraction of the meptyldinocap is 14 percent.
In the present invention, the drug may be a solid preparation or a liquid preparation. Specifically, the pesticide composition can be missible oil, a suspending agent, wettable powder, a dispersed oil suspending agent, water dispersible granules, a microemulsion, powder, granules, an aqueous solution or poison bait. In the embodiment of the invention, the bactericidal composition is prepared into a suspending agent to verify the bactericidal activity of the bactericidal composition.
In the suspending agent, the auxiliary materials acceptable in the pesticide comprise water, a dispersing agent, a wetting agent, a thickening agent, a preservative and an antifreeze agent. In the embodiment of the invention, the suspending agent is a microcapsule suspending agent, and the auxiliary material further comprises an encapsulating material. In some embodiments, the pesticide comprises 19% to 27% of the bactericidal composition of the present invention, 3% to 9% of a dispersant, 2% to 7% of a wetting agent, 0.1% to 0.7% of a thickener, 1% to 4% of a preservative, 1% to 7% of an antifreeze, 0% to 10% of an encapsulating material, and the balance water. Wherein the dispersant includes but is not limited to sodium lignosulfonate, the wetting agent includes but is not limited to isomeric lauryl polyoxyethylene ether, sodium lauryl sulfate, the thickener includes but is not limited to xanthan gum, the preservative includes but is not limited to sodium benzoate, the antifreeze includes but is not limited to ethylene glycol, and the encapsulating material includes but is not limited to urea formaldehyde resin. The encapsulating material is selected from at least one of polymeric phenol-formaldehyde resin, urea-formaldehyde resin, melamine-formaldehyde resin and calcium alginate.
The microcapsule suspending agent has the advantages of long lasting period, reduced drug consumption, labor and time saving, avoidance of phytotoxicity, improvement of product safety, elimination of peculiar smell and isolation from adverse environment.
In some embodiments, the medicament comprises: 8% of fluoroandenazole bacterium amine, 15% of meptyldinocap, 5% of polyvinyl alcohol-resorcinol polymerized phenolic resin, 4.5% of sodium lignosulfonate, 1.5% of isomeric dodecyl alcohol polyoxyethylene ether, 0.2% of xanthan gum, 1.5% of sodium benzoate, 5% of ethylene glycol and the balance of water.
In some embodiments, the medicament comprises: 8% of fluroindolizidine, 15% of meptyldinocap, 7% of urea-formaldehyde resin, 5% of sodium lignosulfonate, 3% of isomeric dodecyl polyoxyethylene ether, 0.2% of xanthan gum, 1.8% of sodium benzoate, 4% of ethylene glycol and the balance of water.
In some embodiments, the medicament comprises: 8% of fluoroandenazole bacterium amine, 15% of meptyldinocap, 8% of sodium lignosulfonate, 2% of sodium dodecyl sulfate, 8% of urea-formaldehyde resin, 2.7% of isomeric dodecyl alcohol polyoxyethylene ether, 0.3% of xanthan gum, 1.6% of sodium benzoate, 4% of ethylene glycol and the balance of water.
In some embodiments, the medicament comprises: 8% of fluoroandenazole bacterium amine, 15% of meptyldinocap, 7.2% of sodium lignosulfonate, 2.4% of sodium dodecyl sulfate, 10% of calcium alginate, 2.9% of isomeric dodecyl alcohol polyoxyethylene ether, 0.37% of xanthan gum, 1.63% of sodium benzoate, 5% of ethylene glycol and the balance of water.
The invention also provides a method for preventing and treating late blight, which is to apply the medicament.
With the suspending agents described in the examples, the mode of application described in the present invention is spray application. The applied preparation dosage is 5-20 mL/mu, and 30kg of water is used per mu. Specifically, the application adopts a secondary dilution method, and 30kg of water is used for diluting every 5-20 mL of preparation. The diluted solution is applied once in the early stage or the initial stage of late blight, and is applied once again 7-10 days after application, and the application amount of the two times is 30 kg/mu.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the triflumizole amine and the meptyldinocap are compounded, so that the two can generate a synergistic effect, and the formed sterilization composition has a good sterilization effect, and particularly has a good prevention and treatment effect on the cherry tomato late blight. The microcapsule suspension prepared from the composition has the characteristics of long lasting period, low dosage, environmental friendliness, no peculiar smell and high safety.
Detailed Description
The invention provides a bactericidal composition and application thereof, and a person skilled in the art can use the content for reference and appropriately improve the process parameters to realize the bactericidal composition. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The test materials adopted by the invention are all common commercial products and can be purchased in the market. Wherein:
the thiabendazole amine (fluindapyr) belongs to pyrazole-4-amide compounds, and is a succinate dehydrogenase inhibitor (SDHI) bactericide, which interferes tricarboxylic acid cycle by inhibiting activity of pathogenic bacteria succinate dehydrogenase to finally cause death of pathogenic bacteria, and is mainly applied to prevention and treatment of high-class fungi such as basidiomycetes.
The mechanism of action of Meptyldinocap is uncoupling mitochondrial oxidative phosphorylation, so that pathogenic bacteria mitochondrial membrane generates a gap, thereby causing H+Directly enter the mitochondria to destroy the inside and outside H of the membrane+The normal gradient concentration of the ATP causes the normal acceleration of the respiration in cells, the ATP production is stopped, and finally the metabolism of pathogenic bacteria is stopped and then the pathogenic bacteria die. Mainly plays a role in protection and treatment in a contact killing way, and has better effect on fungi such as ascomycetes and the like of powdery mildew.
The invention is further illustrated by the following examples:
example 1
The embodiment of the invention provides a sterilization composition, which comprises the effective components of penflufen and meptyldinocap, wherein the mass ratio of the penflufen to the meptyldinocap is 6: 17.
Example 2
The embodiment of the invention provides a sterilization composition, which comprises the effective components of penflufen and meptyldinocap, wherein the mass ratio of the penflufen to the meptyldinocap is 7: 16.
Example 3
The embodiment of the invention provides a sterilization composition, which comprises the effective components of penflufen and meptyldinocap, wherein the mass ratio of the penflufen to the meptyldinocap is 8: 15.
Example 4
The embodiment of the invention provides a sterilization composition, which comprises the effective components of penflufen and meptyldinocap, wherein the mass ratio of the penflufen to the meptyldinocap is 9: 14.
Example 5
The embodiment of the invention provides a sterilization composition, which comprises the effective components of penflufen and meptyldinocap, wherein the mass ratio of the penflufen to the meptyldinocap is 10: 13.
Experimental example 1 composition bioactivity test
According to indoor bioassay test criteria NY/T1156.12-2008 and NY/T1156.6-2006 of pesticides, the optimal mixing mass ratio of two bactericides is screened by measuring the effect of the mixture of the thiabendazole and the meptyldinocap and different mass ratios of the thiabendazole and the meptyldinocap on the cherry tomato late blight.
Phytophthora infestans (Phytophthora infestans) is provided by the separation and preservation of the Anhui Fengle agro-chemical technology center; the test agents are a triflumizole amine raw drug and a meptyldinocap raw drug; fluindacamide: the mixing proportion of the meptyldinocap is as follows: 6: 17; 7: 16; 8: 15; 9: 14; 10: 13; the composition was formulated into a liquid medicine by dissolving in methanol at the formulation concentrations shown in table 1:
TABLE 1 dosage ratio of each substance of the bactericidal composition
Under aseptic operation conditions, quantitatively adding a pre-melted sterile culture medium into an aseptic conical flask according to test treatment, quantitatively sucking liquid medicines from low concentration to high concentration in sequence, respectively adding into the conical flasks, and sufficiently shaking. Then pouring the mixture into 4 culture dishes with the diameter of 9cm in equal amount to prepare a drug-containing flat plate with corresponding concentration, after the sporangia are generated, washing the sporangia with sterile water, filtering the sporangia with double-layer gauze, placing the sporangia at a low temperature of 4 ℃ in the dark for 3 hours to release zoospores, and directly preparing the zoospores into a spore solution for use. Inoculating with spore suspension 24h before experimental treatment, continuously treating with light/dark 12h every day after inoculation, and culturing at room temperature and relative humidity of 90% for 7 d. Seven treatments are set for the test, the treatment of 0.1% tween 80 aqueous solution containing the same amount of acetone solution is used as a blank control, the liquid medicine is uniformly sprayed on the leaf surfaces until the leaf surfaces are completely wet, the liquid medicine is naturally dried for standby, and the treatment is repeated for 4 times each time.
After the blank control reaches 50%, investigating the disease condition of each treatment in a grading way, calculating the control effect of each treatment concentration according to a formula (1) according to the investigation result, wherein the unit is percentage (%), and reserving two digits after decimal point in the calculation result:
in the formula:
i, preventing and treating effect;
D0-a placebo disease index;
Dt-agent treatment disease index.
Statistical analysis:
performing regression analysis according to the log of each drug concentration and corresponding disease index, and calculating EC of each drug50、EC90And 95% confidence limits.
And calculating the synergy coefficient (SR) of the mixture according to the Wadley method, and evaluating the combined action type of the mixture. The synergistic effect of the mixed medicaments is evaluated according to the synergistic coefficient (SR), namely, the SR < 0.5 is antagonistic action, the SR < 0.5 and less than or equal to 1.5 are additive action, and the SR > 1.5 is synergistic action. The synergistic coefficient (SR) is calculated according to the following formulas (2) and (3):
in the formula:
X1mixture EC50Theoretical values in milligrams per liter (mg/L);
PA-percentage of a in the admixture;
PB-percentage of B in the admixture;
A-EC of A in the mixture50Values in milligrams per liter (mg/L);
B-EC of B in the mixture50Values are in milligrams per liter (mg/L).
In the formula:
SR-synergistic coefficient of the mixture;
X1mixture EC50Theoretical values in milligrams per liter (mg/L);
X2mixture EC50Measured in milligrams per liter (mg/L).
The test results are shown in the following table 2:
TABLE 2 test results
According to the results, the single agents of the flurazole and the meptyldinocap and the mixing proportion thereof have obvious bactericidal effect on the cherry tomato late blight. The penflufen and the meptyldinocap are mixed to show synergistic effect under test mixture ratio, wherein the synergistic effect is highest when the ratio of the penflufen to the meptyldinocap is 8:15, the synergistic coefficient is 5.54, and LC (liquid chromatography) is adopted50Is 0.7526mg/L, LC90It was 3.7501 mg/L.
Example 6
In order to further improve the drug effect, the bactericidal composition with the ratio of the fluroindolizumab to the meptyldinocap of 8:15 is prepared into the microcapsule suspending agent, the specific preparation process is that 8% of fluroindolizumab and 15% of meptyldinocap are pumped into a preparation kettle, corresponding encapsulating materials are added under the condition of high-speed shearing and stirring, after a period of time of stirring, sodium lignosulfonate, isomeric dodecyl polyoxyethylene ether, xanthan gum, sodium benzoate and ethylene glycol are sequentially added, the viscosity, the antibacterial performance and the like of the preparation are regulated and controlled, finally, the water is added to 100%, and the microcapsule suspending agent is prepared after stirring for a proper time, wherein the preparation processes of examples 7-9 are the same as those of example 6.
The ingredients are as follows in table 3:
TABLE 3 the ratio of the components in the bactericidal composition
| Name of ingredient | Content (%) |
| Fluindazole amine | 8 |
| Nitrobenzoyl ester | 15 |
| Polyvinyl alcohol-resorcinol polymeric phenolic resin | 5 |
| Lignosulfonic acid sodium salt | 4.5 |
| Isomeric dodecyl alcohol polyoxyethylene ether | 1.5 |
| Xanthan gum | 0.2 |
| Sodium benzoate | 1.5 |
| Ethylene glycol | 5 |
| Water (W) | The amount to make up 100% |
Example 7
In order to further improve the drug effect, the bactericidal composition with the ratio of the flurazole to the meptyldinocap being 8:15 is prepared into a microcapsule suspending agent, and the components are as follows in percentage by mass:
TABLE 4 composition of bactericidal composition
Example 8
In order to further improve the drug effect, the bactericidal composition with the ratio of the flurazole to the meptyldinocap of 8:15 is prepared into a microcapsule suspending agent, and the components are as follows in percentage by mass:
TABLE 5 composition of the composition
| Name of ingredient | Content (%) |
| Fluindazole amine | 8 |
| Nitrobenzoyl ester | 15 |
| Lignosulfonic acid sodium salt | 8 |
| Sodium dodecyl sulfate | 2 |
| Melamine formaldehyde resin | 8 |
| Isomeric dodecyl alcohol polyoxyethylene ether | 2.7 |
| Xanthan gum | 0.3 |
| Sodium benzoate | 1.6 |
| Ethylene glycol | 4 |
| Water (W) | The amount to make up 100% |
Example 9
In order to further improve the drug effect, the bactericidal composition with the ratio of the flurazole to the meptyldinocap of 8:15 is prepared into a microcapsule suspending agent, and the components are as follows in percentage by mass:
TABLE 6 composition of bactericidal composition
Experimental example 2 test of physicochemical Properties of microcapsule suspension
The encapsulation efficiency refers to the rate of the active ingredients encapsulated by the polymer material, and the active ingredients free from the microcapsules generally do not have the effects of reducing the odor, stabilizing the active ingredients, prolonging the effect duration and the like of the microcapsule granules, so the encapsulation efficiency is an important index of the microcapsule suspending agent.
The method for measuring the encapsulation efficiency comprises the steps of weighing 1g (accurate to 0.0002g) of the microcapsule suspension preparation prepared in the embodiment 6-9, adding the weighed microcapsule suspension preparation into a centrifugal tube with a plug, adding 9ml of petroleum ether or methanol and the like, uniformly mixing, centrifuging by a high-speed centrifuge (1200 r/min,5min), sucking supernatant, extracting for 3 times, combining extract liquor, fixing the volume by using the petroleum ether or the methanol and the like, and measuring the mass analysis of the effective components (a). And (b) breaking the capsule of the precipitate by using an ultrasonic oscillator for ultrasonic treatment (200Hz,5min), fixing the volume, measuring the mass of the effective components, analyzing (b), and calculating the encapsulation efficiency.
The encapsulation efficiency calculation formula (%) is a/(a + b). times.100%
TABLE 7 encapsulation efficiency assay results for microcapsule suspension
| Item | Fluindazole amine encapsulation efficiency (%) | Nitrophenyl ester encapsulation efficiency (%) |
| Example 6 | 88.5 | 87.2 |
| Example 7 | 84.6 | 85.8 |
| Example 8 | 95.7 | 95.9 |
| Example 9 | 86.5 | 85.3 |
| Example 10 | 82.6 | 81.4 |
From the above results, the encapsulation efficiency of the microcapsule suspension prepared in examples 6 to 9 can reach more than 80%, and the encapsulation efficiency of the microcapsule suspension prepared in example 8 exceeds 95%, and the stability of the dosage form is the highest.
Experimental example 3 field drug efficacy test
In the field efficacy test I, the bactericidal composition prepared in the application example 8 is used for preventing and controlling cherry tomato late blight in the field.
1. The test method comprises the following steps:
the test agents are shown in table 8 below:
TABLE 8 Scale for each drug test
The experimental plot is arranged in Fedong county of Hefei city, Anhui province, and the sowing condition is as follows: the tomatoes are sown in 3 and 25 days in 2020, and are transplanted in 4 and 25 days in 2020, the row spacing is about 50 cm, the plant spacing is about 30 cm, 3000 plants are transplanted per mu, and the growth of the tomatoes in the field is uniform. Equal amount of clear water is sprayed in the blank control area, the dosage of each cell is calculated in the medicament treatment area according to the medicament dosage set in the test, a secondary dilution method is adopted, 30kg of water is used in each mu of land, and each group of medicaments (the treatment volume is 1-5 mL, 10mL, 20mL, 50mL and 60mL in sequence) in the table 6 are all diluted by 40kg of water. The diluted solution is applied once in the early stage or the initial stage of the tomato late blight, and is applied once 7 to 10 days after the application, the application amount of the two applications is 30 kg/mu, and the group 6 is given with the same amount of water. Uniformly spraying the water from low concentration to high concentration one by one at a constant speed without re-spraying or missing spraying. The sprayers are washed by clean water before application in different medicament treatment rooms. The application time and the crop growth period are as follows: the first medication is 6 months and 24 days in 2020, and tomatoes are in the fruiting period; the second dose is 7/4/2020, and 10 days after the first dose, cherry tomato is in fruiting period.
And (5) investigating the drug effect for 2 times.
First investigation: performing the first investigation of the incidence of the cherry tomato late blight leaf after the second administration (7/2/7/2020);
and (4) second investigation: investigating the leaf morbidity and fruit morbidity of the cherry tomato late blight 14 days after the second medicine (7 months and 16 days in 2020);
2 results of the test
The control effect of different medicament treatments on the cherry tomato late blight is shown in the following table 9.
TABLE 9 prevention and treatment effects of different drug treatments on cherry tomato late blight
According to the table, the results of the control effect survey show that the control effect of the preparation with the dosage of 30 ml/mu is the best in the example treatment 8, the control effect of the preparation after the second time is 81.02% in 14 days, and the drug effect of the lower, middle and high three doses is obviously better than that of the two control medicaments under the condition that the dosage is obviously lower than that of the control medicaments. According to field observation, the tested medicament and the control medicament are safe to cherry tomatoes of test crops, and no phytotoxicity symptoms (such as dwarfing, chlorosis, deformity and the like) are found.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that it is obvious to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.
Claims (10)
1. The bactericidal composition is characterized by consisting of the fluoroandenconazole amine and the meptyldinocap, wherein the mass ratio of the fluoroandenconazole amine to the meptyldinocap is (6-10) to (13-17).
2. The bactericidal composition of claim 1, wherein the mass ratio of the triflumizole amine to the meptyldinocap is 6:17, 7:16, 8:15, 9:14 or 10: 13.
3. Use of the fungicidal composition of claim 1 or 2 for the inhibition of phytophthora.
4. Use according to claim 3, wherein the mould is Phytophthora infestans.
5. The use of the fungicidal composition according to claim 1 or 2 for controlling late blight of crops.
6. The use of claim 5, wherein the late blight is cherry tomato late blight.
7. A medicament for preventing and treating late blight, which comprises the bactericidal composition of claim 1 or 2 and an auxiliary acceptable in pesticides.
8. The drug according to claim 7, which is in the form of microcapsule suspension, wherein the microcapsule suspension comprises the following components in percentage by mass:
19-27% of the bactericidal composition of claim 1 or 2, 3-9% of a dispersant, 2-7% of a wetting agent, 0.1-0.7% of a thickening agent, 1-4% of a preservative, 1-7% of an antifreeze agent, 5-10% of an encapsulating material, and the balance of water.
9. The drug of claim 8, wherein the encapsulating material is at least one selected from the group consisting of a polymeric phenol-formaldehyde resin, a urea-formaldehyde resin, a melamine-formaldehyde resin, and calcium alginate.
10. A method for controlling late blight, characterized by administering the agent according to any one of claims 7 to 9.
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| EP3412150A1 (en) * | 2017-06-06 | 2018-12-12 | Basf Se | Mixtures of meptyldinocap with sdhi fungicides |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP3412150A1 (en) * | 2017-06-06 | 2018-12-12 | Basf Se | Mixtures of meptyldinocap with sdhi fungicides |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669669A (en) * | 2022-11-17 | 2023-02-03 | 江苏七洲绿色化工股份有限公司 | Composition containing indoxacarb and tebuconazole as well as preparation method and application thereof |
| CN115669669B (en) * | 2022-11-17 | 2024-04-23 | 江苏七洲绿色化工股份有限公司 | Composition containing penconazole and tebuconazole and preparation method and application thereof |
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