CN110174386A - 一种检测精胺的主客体传感对及其应用 - Google Patents
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Abstract
本发明公开了一种在生理环境中检测精胺的传感对探针，是包括主体磺化杯[4]芳烃和客体6,7,9,14a‑四氢吡啶并[1,2‑a]吡啶并[1'2'3'4']咪唑并[2,1‑d]吡嗪‑5,14‑二鎓(PMP)的传感对，如下所示:
Description
【技术领域】
本发明属于主客体荧光探针领域，特别是一种检测精胺的主客体传感对及其应用。
【背景技术】
精胺作为一种小型阳离子多胺，多存在于细菌，植物，动物组织和体液中。相关研究表明，精胺和亚精胺与细胞分裂、生长、存活过程存在密切联系。实验发现抑制或消耗动物细胞中的多胺可致使RNA翻译和胚胎发育发生停滞，终止细胞周期，抑制细胞分化。而且，在肿瘤细胞中，多胺与多胺合成酶的含量会异常升高，机体内多胺稳态的紊乱与癌症的发生发展密切相关。所以，测定血液或尿液中的精胺可以在临床上作为一种恶性肿瘤诊断指标。用于检测精胺的传统分析技术包括免疫测定，高效液相色谱法(HPLC)，离子交换液相色谱法和光谱法。然而，它们通常需要进行一些复杂，繁琐和耗时的预处理。而在之前已经报道过的精胺检测传感对中，所用到的荧光染料光泽精在遇到氯离子时会发生荧光淬灭的现象，这就使得该传感对不能在pbs等生理缓冲溶液中进行检测，限制了其在生理条件下的应用。所以，发展一种简单，有效和快速的检测精胺的方法仍然是非常必要的。
【发明内容】
本发明的目的是针对上述技术分析和存在问题，开发了一种简单而新颖的传感对探针，克服了其他精胺检测传感对在生理环境无法应用的缺点，能在更贴近生物环境的体系中超灵敏快速的检测精胺。
本发明的技术方案：
一种检测精胺的探针，其特征在于，在贴近生理环境的缓冲溶液pbs中，利用荧光竞争滴定的方法，利用精胺和大环主体之间的强络合作用，置换出荧光剂使其发光。
(1)荧光剂结构：
(2)主体结构：
上述化合物的具体名称，荧光剂：6,7,9,14a-四氢吡啶并[1,2-a]吡啶并[1'2'3'4']咪唑并[2,1-d]吡嗪-5,14-二鎓(PMP)，主体：磺化杯[4]芳烃
其制备方法为：
(1)吡啶-2-甲醛和溴代乙基氨基氢溴酸盐发生亲核加成反应生成PMP。
(2)以对叔丁基苯酚为原料，和甲醛发生分子间缩合反应得到对叔丁基杯[4]苯酚。对叔丁基苯酚在三氯化铝的催化下发生傅-克反应逆反应得到杯[4]芳烃，之后发生磺化反应生成磺化杯[4]芳烃SC4A。
上述两种化合物的具体制备方法均为现有技术，在此不再赘述，本发明所述的两种化合物均采用以上合成路线。
根据本发明的第二方面，本发明提供包含上述两种化合物的传感对，所述传感对用于检测精胺。
本传感对探针采用荧光竞争滴定方法检测精胺。
进一步具体的，本传感对探针对精胺的检测是在磷酸盐缓冲溶液(PBS)和水中。
本发明所述的传感对可以通过简单的荧光检测的方法检测精胺，在生理环境中实现超灵敏快速的定量检测精胺。该探针运用SC4A和PMP组成传感对，运用荧光竞争滴定的方法检测生理缓冲中的精胺。
【附图说明】
图1为不同浓度的荧光染料PMP的相对荧光强度。
图2为不同pH条件下荧光染料PMP的相对荧光强度。
图3为不同浓度Cl-条件下荧光染料PMP的相对荧光强度
图4为传感对探针溶液中加入不同浓度精胺的荧光光谱。
图5为精胺的浓度和463nm处的荧光强度之间的非线性拟合。
【具体实施方式】
实施例1
PMP的相对荧光强度和其浓度之间的关系：
用微量注射器配制好的不同浓度的PMP溶液，分别检测其相对荧光强度，得到染料荧光强度随染料浓度的变化曲线，如图1.
PMP的相对荧光强度和体系pH之间的关系：
首先，配置不同pH的pbs缓冲溶液，在pH为7.2的pbs缓冲溶液中分别加入盐酸溶液和氢氧化钠溶液，利用pH计监测调节其pH分别为3-13。之后，在不同pH溶液中测试PMP的相对荧光强度，如图2，该图证明染料PMP在生理环境的pH下有着良好的荧光性质。
实施例2：
运用荧光滴定的方法，以SC4A和PMP作为传感对，将SC4A和PMP应用于在PBS中超灵敏选择性定量检测精胺浓度。建立了定量检测生理环境精胺浓度的体系，其具体实施步骤如下：在25℃温度下，将SC4A、精胺和PMP溶解到水中配成1mM的母液待用。将SC4A和染料PMP以用10mmol/L pH为7.4的PBS缓冲溶液分别稀释到1μmol/L,2μmol/L。得到待滴定的标准溶液。所述待滴定的标准溶液的体积为3mL，此时测得的标准溶液的荧光强度为基准荧光强度；然后向待滴定的标准溶液中逐渐滴加精胺储备液，可以看到荧光信号的恢复。通过这种荧光竞争滴定的方法，可以测得相对荧光强度随精胺浓度变化的竞争曲线，如图3、4。该图证明，在接近生理环境的PBS溶液中精胺和SC4A有6×104M-1的键合常数，证实了该体系在超灵敏定量检测精胺浓度的可行性。
Claims (3)
1.一种可以在生理环境中检测精胺的传感对，其特点在于：克服了传统荧光染料光泽精在氯离子存在下荧光淬灭的缺陷，能在PBS缓冲溶液中以及生理环境中检测精胺，其结构如下:
2.权利要求书1所述的传感对在检测样品中精胺的应用。
3.如权利要求2所述的应用，其特征在于：所述含精胺样品是生物细胞或含精胺的溶液。
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| CN112608256A (zh) * | 2020-12-03 | 2021-04-06 | 中山蔚蓝医疗器械有限公司 | 一种aie探针及其制备方法和应用 |
| CN115266671A (zh) * | 2022-08-02 | 2022-11-01 | 四川农业大学 | 一种用于食品新鲜度检测的三发射传感探针 |
| JP2023151151A (ja) * | 2022-03-31 | 2023-10-16 | 邦明 長峯 | ポリアミンの検出方法、ポリアミンの検出試薬及びアプタマ |
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