CN110151891A - A kind of feed addictive improving stock cock reproductive performance - Google Patents

A kind of feed addictive improving stock cock reproductive performance Download PDF

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Publication number
CN110151891A
CN110151891A CN201910510638.2A CN201910510638A CN110151891A CN 110151891 A CN110151891 A CN 110151891A CN 201910510638 A CN201910510638 A CN 201910510638A CN 110151891 A CN110151891 A CN 110151891A
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additive
stock cock
improve
specially
cock
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吴立波
王军
王伟利
牛淑玲
宫永胜
于曦
张志宝
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Jilin Blue Fusion Biotechnology Co Ltd
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Jilin Blue Fusion Biotechnology Co Ltd
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Priority to CN201910510638.2A priority Critical patent/CN110151891A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/41Crassulaceae (Stonecrop family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/54Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • AHUMAN NECESSITIES
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/57Magnoliaceae (Magnolia family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/65Paeoniaceae (Peony family), e.g. Chinese peony
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/71Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/79Schisandraceae (Schisandra family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/80Scrophulariaceae (Figwort family)
    • A61K36/804Rehmannia
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/884Alismataceae (Water-plantain family)
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/894Dioscoreaceae (Yam family)
    • A61K36/8945Dioscorea, e.g. yam, Chinese yam or water yam
    • AHUMAN NECESSITIES
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    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis

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Abstract

The invention discloses a kind of additives for improving stock cock reproductive performance.The effective component of the additive includes Rehmannia glutinosa, Fructus Corni, Chinese yam, cortex moutan, Poria cocos, rhizoma alismatis, root of kirilow rhodiola, fructus schisandrae, Herba Epimedii and cortex cinnamomi.Concrete content are as follows: Rehmannia glutinosa: 19~23%, Fructus Corni: 10~12%, Chinese yam: 10~12%, cortex moutan: 8~10%, Poria cocos: 8~10%, rhizoma alismatis: 8~10%, root of kirilow rhodiola: 8~10%, fructus schisandrae: 8~10%, Herba Epimedii: 5~7%, cortex cinnamomi: 5~7%.The adaptability that can reinforce body is added in diet according to the amount ratio 14g:1kg of additive and animal and fowl fodder, improves anti-stress ability, improves stock cock semen quality, cock can be helped to alleviate the sperm as caused by high frequency semen collection rare.Stock cock hatching criteria is improved, and then improves its reproductive performance.

Description

A kind of feed addictive improving stock cock reproductive performance
Technical field
The invention belongs to feed additive fields, are related to a kind of feed addictive for improving stock cock reproductive performance.
Background technique
In kind of chicken house, male and female chicken carries out cultivating by a certain percentage and select and remain, and the semen quality of stock cock directly affects The rate of fertilization of hatching egg and the quality of chick, that is, directly affect the economic benefit for planting chicken house.Modern intensive breeding way is same When be stressor again, can make chicken group generate stress, cause body void to lose retardance, kidney yang deficiency, endocrine disturbance, nutriment enters It does not apply, some stock cocks is caused can not adopt sperm or semen quality difference and be eliminated.In order to solve this problem, a large amount of artificial Feed addictive of synthesis such as hormone preparation, antibiotic etc. generally uses in production, but this kind of additive easily in vivo residual, It develops drug resistance, causes environmental pollution, bring adverse effect to the health of the mankind.Chinese herbal medicine have its natural sex, multifunctionality, Less toxic side effect, low medicine be residual, unique advantage without drug resistance, and human body immune function can be enhanced, improve breeding performonce fo animals, have A little Chinese herbal medicines are also containing sterols, can improve the reproductive performance of livestock and poultry, be to substitute synthetics and steroids Ideal feed addictive.
Summary of the invention
The object of the present invention is to provide a kind of feed addictives for improving stock cock reproductive performance.
Feed addictive provided by the invention, effective component include Rehmannia glutinosa, Fructus Corni, Chinese yam, cortex moutan, Poria cocos, Rhizoma alismatis, root of kirilow rhodiola, fructus schisandrae, Herba Epimedii and cortex cinnamomi.
Specifically, the percentage composition difference that each component accounts for the additive gross mass is as follows in the additive:
Rehmannia glutinosa: 19~23%,
Fructus Corni: 10~12%,
Chinese yam: 10~12%,
Cortex moutan: 8~10%,
Poria cocos: 8~10%,
Rhizoma alismatis: 8~10%,
Root of kirilow rhodiola: 8~10%,
Fructus schisandrae: 8~10%,
Herba Epimedii: 5~7%,
Cortex cinnamomi: 5~7%.
Specifically, the additive is grouped as by each group of following each mass percentage:
Rehmannia glutinosa 21%, Fructus Corni 11%, Chinese yam 11%, cortex moutan 9%, Poria cocos 9%, rhizoma alismatis 9%, root of kirilow rhodiola 9%, north Schisandra chinensis 9%, longspur epimedium 6%, cortex cinnamomi 6%.
The method for preparing additive that aforementioned present invention provides, includes the following steps:
Aforementioned each component is mixed according to the proportion, is ground into powder, obtains the additive.
In addition, application and the additive of the additive of aforementioned present invention offer in animal and fowl fodder are as animal and fowl fodder Additive in application, also belong to protection scope of the present invention.Wherein, the amount ratio of the additive and animal and fowl fodder is 12 ~16g:1kg, specially 14g:1kg;
The livestock and poultry are chicken, specially stock cock.
The application for the additive that aforementioned present invention provides, for the application in the reproductive performance for improving stock cock.Specially It is following 1) to any one in 2):
1) in the application for improving stock cock semen quality;
2) in the application for improving stock cock hatching criteria.
In addition, using above-mentioned additive provided by the invention to be any one in the product with following function of effective component Kind, also belong to protection scope of the present invention:
3) stock cock semen quality is improved;
4) stock cock hatching criteria is improved.
Wherein, the amount ratio of the additive and animal and fowl fodder is 12~16g:1kg, specially 14g:1kg;
The livestock and poultry are chicken, specially stock cock.
It is described 1) in, improve stock cock semen quality be specially improve stock cock volume of semen, improve stock cock sperm concentration, At least one of improve stock cock sperm motility and reduce stock cock rate of teratosperm;
It is described 2) in, improving stock cock hatching criteria is specially to improve fertility rate of hatching egg, improve and hatchability of fertile eggs and mention At least one of high chick rate;
It is described 3) be specially improve stock cock volume of semen, improve stock cock sperm concentration, improve stock cock sperm motility and Reduce at least one of stock cock rate of teratosperm;
It is described 4) be specially improve stock cock hatching criteria be specially improve fertility rate of hatching egg, improve hatchability of fertile eggs and Improve at least one of chick rate.
Rehmannia glutinosa, Fructus Corni, fructus schisandrae, Herba Epimedii in feed addictive of the invention can tonify Qi of the kidney;Cortex cinnamomi exists Feed palatability can be improved while warm kidney yang-tonifying;Chinese yam, Poria cocos invigorating the spleen excreting dampness;It is evil that rhizoma alismatis lets out water in kidney;Cortex moutan clearing liver gallbladder Xiang Huo;Root of kirilow rhodiola anoxia and fatigue-resisting, reinforces the adaptability of body, improves anti-stress ability, can help cock alleviate due to Sperm caused by high-frequency semen collection is rare.The combination of ten kinds of Chinese herbal medicines can be improved the semen quality and fertile egg of stock cock Hatching criteria, on the whole improve stock cock reproductive performance.
Specific embodiment
The present invention is further elaborated combined with specific embodiments below, but the present invention is not limited to following embodiments.Institute State method is conventional method unless otherwise instructed.The raw material can obtain unless otherwise instructed from public commercial source.Under It states content described in embodiment unless otherwise instructed, is mass percentage.
In following embodiments, Chinese herbal medicine used is purchased from Anguo City brightness hair prepared slices of Chinese crude drugs processing Co., Ltd.
Embodiment 1 supplements influence of the additive to stock cock reproductive performance in feed
Test material
Feed addictive ingredient is as follows, Rehmannia glutinosa 21%, Fructus Corni 11%, Chinese yam 11%, cortex moutan 9%, Poria cocos 9%, Rhizoma alismatis 9%, root of kirilow rhodiola 9%, fructus schisandrae 9%, longspur epimedium 6%, cortex cinnamomi 6%.
Experimental design and animal
37 week old yellow-feather broiler stock cocks 50 similar in health, weight, the meat hen of AA+ 250 are selected in this test.With Machine is divided into 2 groups, every group of 25 cocks and 125 hens, if 5 repetitions, 5 stock cocks of each repetition and 25 hens.Test It is carried out in same henhouse, keeps feeding environment consistent.1.4% feed addition is additionally added in test group basal diet Agent, control group normally feed basal diet, and experimental period is 60 days.
Test diet
Basal diet is provided by Long Sheng feed corporation,Ltd, Jilin Province.Diet composition and trophic level are shown in Table 1.
1 diet of table composition and trophic level
Feeding management
In June, 2015, test are carried out in the bright and sharp farm in Changchun, the raising of test chicken single cage.During test, test chicken is free It drinks water, feeds 3 times (6:00,12:00,18:00) daily, few feed frequently adds, and feeds amount showed a small surplus in hopper as degree.Timing in house Ventilation keeps cleaning in house, controls temperature at 18-20 DEG C.
Index determining
(1) semen quality
Volume of semen: sperm is collected with the smart cup of graduated collection, is directly read on collecting smart cup, measured value is accurate to 0.01ml。
Sperm concentration: it is counted with red-cell count plate.
Sperm motility: diluting 400 times for 0.9% sodium chloride of the sperm of acquisition, shake mixing 30 seconds, then will dilution Drop is on glass slide, covered, the movement feelings of 500 sperms under 400 times of the microscopically observation 5 different visuals field Condition records ratio shared by the sperm count of straight ahead movement.Sperm viability scoring is carried out with 10 grades of point systems.
Rate of teratosperm: taking the sperm of 200 times of dilution on glass slide, spontaneously dry after smearing, then imperial with 0.5% alcohol Gallbladder purple dyes 3min, washes away extra dyestuff, dries rear microscopy, and contained defective sperm number in 100 sperms of casual inspection calculates Abnormal rate.
(2) hatching criteria
Fertility rate of hatching egg: when entering to incubate hatching of breeding eggs to 10d, according to egg, infertile egg is rejected, calculates fertility rate of hatching egg=fertilization kind Egg number/enter incubation number × 100%.
Hatchability of fertile eggs: it when hatching 21d is incubated out, calculates hatchability of fertile eggs=go out and incubate baby chick number/and enters the fertilization kind incubated Egg number × 100%.
Chick rate: it when hatching 21d is incubated out, calculates chick rate=the young number of health/and goes out and incubate baby chick number × 100%.
Data statistics and analysis
All data of this test using SPSS16.0 Software of Data Statistics carry out one-way analysis of variance, with LSD method into Row Multiple range test, test data are indicated with " average value ± standard error ".
As a result
As shown in Table 2, compared with the control group, in feed supplement additive it is extremely significant improve stock cock volume of semen (P < 0.01) stock cock sperm concentration (P < 0.05), is significantly improved, and is improved sperm motility and reduces becoming for rate of teratosperm Gesture.Improve the semen quality of stock cock.
As shown in Table 3, compared with the control group, in feed supplement additive improve extremely significantly fertility rate of hatching egg (P < 0.01), and it is improved the trend of hatchability of fertile eggs and chick rate, thus additive has significantly the hatching criteria of stock cock Improvement result.
Influence of the table 2 by supplement additive to stock cock semen quality
Note:a,bDifferent letters of going together indicate significant difference (P < 0.05),A,BGo together it is different letter indicate difference it is extremely significant (P < 0.01)。
Influence of the table 3 by supplement additive to stock cock hatching criteria
Note:a,bDifferent letters of going together indicate significant difference (P < 0.05),A,BGo together it is different letter indicate difference it is extremely significant (P < 0.01)。
Embodiment 2 supplements influence of the additive to stock cock reproductive performance in feed
Test material
Additive component is as follows, Rehmannia glutinosa 21%, Fructus Corni 11%, Chinese yam 11%, cortex moutan 9%, Poria cocos 9%, rhizoma alismatis 9%, root of kirilow rhodiola 9%, fructus schisandrae 9%, longspur epimedium 6%, cortex cinnamomi 6%.
Experimental design and animal
Test is designed using single-factor, selects egg stock cock 72 of 37 week old, is randomly divided into 4 groups, and I group is control Group, feed basal diet, II, III, IV group added on the basis of basal diet respectively 1.2%, 1.4% and 1.6% additive, Every group of 4 repetitions, 6 stock cocks of each repetition are female with corresponding hen by 1 male 5.Experimental period 28 days.
Test diet
Basal diet is provided by Long Sheng feed corporation,Ltd, Jilin Province.Diet composition and trophic level are shown in Table 4.
4 diet of table composition and trophic level
Feeding management
In April, 2015, test are carried out in the bright and sharp farm in Changchun, the raising of test chicken single cage.During test, test chicken is free It drinks water, feeds 3 times (6:00,12:00,18:00) daily, few feed frequently adds, and feeds amount showed a small surplus in hopper as degree.Timing in house Ventilation keeps cleaning in house, controls temperature at 18-20 DEG C.
Index determining
It acquires stock cock sperm respectively in pre-feeding period end and the 7th, 14,21 and 28 day experimental period and collects hatching egg.It refers specifically to It is as follows to mark measuring method:
(1) semen quality
Volume of semen: sperm is collected with the smart cup of graduated collection, is directly read on collecting smart cup, measured value is accurate to 0.01ml。
Sperm concentration: it is counted with red-cell count plate.
Sperm motility: diluting 400 times for 0.9% sodium chloride of the sperm of acquisition, shake mixing 30 seconds, then will dilution Drop is on glass slide, covered, the movement feelings of 500 sperms under 400 times of the microscopically observation 5 different visuals field Condition records ratio shared by the sperm count of straight ahead movement.Sperm viability scoring is carried out with 10 grades of point systems.
Rate of teratosperm: taking the sperm of 200 times of dilution on glass slide, spontaneously dry after smearing, then imperial with 0.5% alcohol Gallbladder purple dyes 3min, washes away extra dyestuff, dries rear microscopy, and contained defective sperm number in 100 sperms of casual inspection calculates Abnormal rate.
(2) hatching criteria
Fertility rate of hatching egg: when entering to incubate hatching of breeding eggs to 10d, according to egg, infertile egg is rejected, calculates fertility rate of hatching egg=fertilization kind Egg number/enter incubation number × 100%.
Hatchability of fertile eggs: it when hatching 21d is incubated out, calculates hatchability of fertile eggs=go out and incubate baby chick number/and enters the fertilization kind incubated Egg number × 100%.
Chick rate: it when hatching 21d is incubated out, calculates chick rate=the young number of health/and goes out and incubate baby chick number × 100%.
Data statistics and analysis
All data of this test using SPSS16.0 Software of Data Statistics carry out one-way analysis of variance, with LSD method into Row Multiple range test, test data are indicated with average value ± standard error.
As a result
As shown in Table 5, compared with the control group, additive is supplemented in feed, to the sperm motility and teratospermia of stock cock Rate does not make significant difference simultaneously.However, significantly improving the volume of semen and essence of stock cock with the increase of additive level in feed Sub- density (P < 0.05), improves the semen quality of stock cock.
As shown in Table 6, compared with the control group, with the increase of additive level in feed, significantly improve hatching egg by Smart rate and hatchability of fertile eggs (P < 0.05), improve the hatching criteria of stock cock.
Influence of the table 5 by supplement additive to stock cock semen quality
Note:a,bDifferent letters of going together indicate significant difference (P < 0.05).
Influence of the table 6 by supplement additive to stock cock hatching criteria
Note:a,bDifferent letters of going together indicate significant difference (P < 0.05).
And consider the double factor of effect (influence to semen quality and hatching criteria) and cost, additive and feed Adding proportion with 14g:1kg is best.
Analysis
By the result of above-mentioned two test it is found that semen quality and the hatching of stock cock can be improved by supplementing additive in feed Performance, to improve the reproductive performance of stock cock.
Conclusion
Test result shows that the 14g additive is supplemented in 1kg animal and fowl fodder can improve the semen quality of stock cock and incubate Change performance, to improve the reproductive performance of stock cock.

Claims (10)

1. a kind of additive, effective component includes Rehmannia glutinosa, Fructus Corni, Chinese yam, cortex moutan, Poria cocos, rhizoma alismatis, root of kirilow rhodiola, north Schisandra chinensis, Herba Epimedii and cortex cinnamomi.
2. additive according to claim 1, it is characterised in that: in the additive, effective component by the Rehmannia glutinosa, Fructus Corni, Chinese yam, cortex moutan, Poria cocos, rhizoma alismatis, root of kirilow rhodiola, fructus schisandrae, Herba Epimedii and cortex cinnamomi composition.
3. additive according to claim 1 or 2, it is characterised in that: in the additive, each component accounts for the additive The percentage composition difference of gross mass is as follows:
Rehmannia glutinosa: 19~23%,
Fructus Corni: 10~12%,
Chinese yam: 10~12%,
Cortex moutan: 8~10%,
Poria cocos: 8~10%,
Rhizoma alismatis: 8~10%,
Root of kirilow rhodiola: 8~10%,
Fructus schisandrae: 8~10%,
Herba Epimedii: 5~7%,
Cortex cinnamomi: 5~7%.
4. additive according to claim 3, it is characterised in that: the additive is by following each mass percentage Each group is grouped as:
Rehmannia glutinosa 21%, Fructus Corni 11%, Chinese yam 11%, cortex moutan 9%, Poria cocos 9%, rhizoma alismatis 9%, root of kirilow rhodiola 9%, Chinese Magnoliavine Son 9%, longspur epimedium 6%, cortex cinnamomi 6%.
5. a kind of method for preparing additive described in claim 3 includes the following steps: each group described in claim 3 Divide and mix according to the proportion, obtains the additive.
6. application of the additive described in claim 3 in animal and fowl fodder.
7. additive described in claim 3 is as the application in additive for farm animal feed.
8. application according to claim 6 or 7, it is characterised in that: the amount ratio of the additive and animal and fowl fodder is 12 ~16g:1kg, specially 14g:1kg;
The livestock and poultry are chicken, specially stock cock.
It is specially following 1) in 2) for the reproductive performance for improving stock cock 9. the application of additive described in claim 3 Any one:
1) application in stock cock semen quality is being improved;
2) application in stock cock hatching criteria is being improved;
Stock cock reproductive performance product is improved by having for effective component of additive described in claim 3, is specially had such as Any one in the product of lower function:
3) stock cock semen quality is improved;
4) stock cock hatching criteria is improved.
10. application according to claim 9 or product, it is characterised in that: the amount ratio of the additive and animal and fowl fodder For 12~16g:1kg, specially 14g:1kg;
The livestock and poultry are chicken, specially stock cock;
It is described 1) in, improve stock cock semen quality be specially improve stock cock volume of semen, improve stock cock sperm concentration, improve At least one of stock cock sperm motility and reduction stock cock rate of teratosperm;
It is described 2) in, improving stock cock hatching criteria is specially to improve fertility rate of hatching egg, improve hatchability of fertile eggs and improve strong At least one of young rate;
3) described is specially to improve stock cock volume of semen, improve stock cock sperm concentration, improve stock cock sperm motility and reduction At least one of stock cock rate of teratosperm;
4) described is specially to improve stock cock hatching criteria to be specially raising fertility rate of hatching egg, improve hatchability of fertile eggs and raising At least one of chick rate.
CN201910510638.2A 2019-06-13 2019-06-13 A kind of feed addictive improving stock cock reproductive performance Pending CN110151891A (en)

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Application publication date: 20190823