CN110141530A - A kind of converted starch composition and its application - Google Patents

A kind of converted starch composition and its application Download PDF

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Publication number
CN110141530A
CN110141530A CN201910425429.8A CN201910425429A CN110141530A CN 110141530 A CN110141530 A CN 110141530A CN 201910425429 A CN201910425429 A CN 201910425429A CN 110141530 A CN110141530 A CN 110141530A
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converted starch
starch composition
starch
converted
cordate houttuynia
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张晋滔
张晋源
徐远平
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Huazhong Agricultural University
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • A61K8/732Starch; Amylose; Amylopectin; Derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • A61K8/735Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/10Anti-acne agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/008Preparations for oily skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/594Mixtures of polymers

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Abstract

The invention discloses a kind of converted starch compositions, are mixed by cornstarch, cordate houttuynia starch and tapioca according to 1~3:1~5:1~8 ratio, the converted starch composition obtained after denaturing step;And converted starch composition is the high amylose starch that molecular weight is 100000~999999, purity is 92~100%, and converted starch composition is applied in cosmetics.The present invention is mixed with to obtain converted starch composition according to special ratios by cornstarch, cordate houttuynia starch and tapioca, not only has certain anti-inflammatory, antibiotic property, has more higher system stability;The present invention applies converted starch composition in preparing cosmetics, in conjunction with soluble polysaccharide, Flos Nelumbinis extract and grape seed extract, it is able to achieve synergistic oil-control, anti-acne effect, and the stability of system is higher, so that the overall security of anti-acne lotion is higher.

Description

A kind of converted starch composition and its application
Technical field
The present invention relates to starch technical field, in particular to a kind of converted starch composition and its application.
Background technique
Starch is the carbohydrate that plant is formed through photosynthesis, it is from a wealth of sources, cheap, degradation after still with The form of carbon dioxide and water returns to the Nature, it is considered to be the material for the natural reproducible not polluted, in non-edible field Extensive research and development is obtained.It is melted since starch has not dissolving in poor cold water, shear resistant, poor water resistance and lack The disadvantages of mobility, it is modified next to need to carry out it Chemical Physics so that it is difficult to use separately as a kind of high molecular material Enhance certain functions or forms new physicochemical characteristic.Currently, as new converted starch raw material can widen answering for converted starch With field, the development of converted starch is pushed.In addition, starch also has more research in the application of cosmetic field, but will form sediment Powder carry out denaturation be reused in it is less in cosmetics, especially in the cosmetics of anti-acne class.Pass through in existing product for resolving poxes The breeding for inhibiting bacterium eliminates its stimulation and its destruction to tissue, or passes through laser irradiation, quick anti-acne, still It is too big to the stimulation of skin, it cannot fundamentally solve the problems, such as anti-acne or even the big chemical reagent of irritation to impaired skin There is bigger damage.If the raw material that converted starch is produced as anti-acne lotion, itself has thickening, functional work With so that cosmetic product have more stable architecture, indirectly in reduction system preservative use so that product Safer, therefore, studying application of the converted starch in cosmetics has great meaning.
A kind of converted starch composition, the practical problem urgently handled in practical application are specific there are also not proposing very much Solution.
Summary of the invention
In view of the deficiencies of the prior art, on the one hand the purpose of the present invention is to provide a kind of converted starch composition.
In order to achieve the above object, the present invention adopts the following technical scheme:
A kind of converted starch composition, by cornstarch, cordate houttuynia starch and tapioca according to 1~3:1~5:1~8 Ratio mixing, the converted starch composition obtained after denaturing step;And converted starch composition is high amylose Starch, the molecular weight of the high amylose starch are 100000~999999, and purity is 92~100%.
Preferably, the cordate houttuynia starch the preparation method comprises the following steps: the snow-white cordate houttuynia rhizome of selected color, removes root, puts Entering in beater and be beaten, the sodium chloride solution that the concentration of 1~3 times of amount is 5% is added, filtering takes filtrate, 1~3h is stood, Supernatant is detected with silver nitrate, shows non-sodium chloride, centrifugation obtains starch, then be dried in vacuo at 45~50 DEG C, water content control System is below 15%.
Preferably, the denaturing step are as follows: proportionally mix the cornstarch, cordate houttuynia starch and tapioca It closes, is modulated into slurries, the ion being sufficiently swollen is added to 6.0~7.0 in slurries by the salt acid for adjusting pH of addition 0.01~0.03% It in sol solution, stirs evenly to form mixed liquor, mixed liquor is placed in baking oven and is dried, until moisture content is down to 10% hereinafter, so After carry out it is xeothermic reaction obtain converted starch composition.
Preferably, the content of the ion glue accounts for the 3~5% of the converted starch composition.
Preferably, the ion glue is carragheen or guar gum.
Preferably, the mixing time is 20~30min.
Preferably, the temperature of the xeothermic reaction is 45~65 DEG C.
On the other hand, the present invention also provides a kind of converted starch compositions to prepare the application in cosmetics.
Preferably, the cosmetics are anti-acne lotion.
A kind of anti-acne lotion, the ingredient including following mass percent: 2~10% converted starches as described in claim 1 Composition, 0.2~1% soluble polysaccharide, 0.1~0.9% Flos Nelumbinis extract, 0.1~0.5% grape seed extract, 4~16% jiaos Shark alkane, 8~15% coixenolides, 1~5% ethylhexyl palmitate, 2~10% butanediols, 2~15% propylene glycol, 2~10% Collagen, 0.2~2.0% Sodium Hyaluronate, 1~3% glycerol, 0.001~0.003% carbomer, 0.1~0.3% 3 second Hydramine, 0.01~0.03% essence, deionized water surplus.
Compared with prior art, advantageous effects obtained by the present invention are:
1, the present invention is mixed with according to special ratios and is denaturalized by cornstarch, cordate houttuynia starch and tapioca Starch composites not only have certain anti-inflammatory, antibiotic property, have more higher system stability.
2, the present invention converted starch composition is applied in preparing cosmetics, in conjunction with soluble polysaccharide, Flos Nelumbinis extract and Grape seed extract is able to achieve the oil-control for coordinating synergy, anti-acne effect, and the stability of system is higher, so that anti-acne The overall security of liquid is higher.
Specific embodiment
Specific embodiments of the present invention will be further explained below.It should be noted that for these implementations The explanation of mode is used to help understand the present invention, but and does not constitute a limitation of the invention.In addition, invention described below Technical characteristic involved in each embodiment can be combined with each other as long as they do not conflict with each other.
Embodiment 1
A kind of converted starch composition is mixed by cornstarch, cordate houttuynia starch and tapioca according to the ratio of 1:1:1, After the cornstarch, cordate houttuynia starch and tapioca are proportionally mixed, slurries are modulated into, 0.01% hydrochloric acid is added PH to 6.0 is adjusted, slurries are added to 3% carragheen or guar gum for accounting for the converted starch composition being sufficiently swollen, are stirred Mix 20min, form mixed liquor, mixed liquor is placed in baking oven and is dried, until moisture content be down to 10% hereinafter, after in temperature be 45 Xeothermic reaction obtains converted starch composition at DEG C.
And converted starch composition is high amylose starch, the molecular weight of the high amylose starch is 100000~999999, purity 92.5%.
Wherein, the preparation of the cordate houttuynia starch are as follows: the snow-white rhizome of selected color removes root, be put into beater into The sodium chloride solution that the concentration of 1 times of amount is 5% is added in row mashing, and filtering takes filtrate, stands 1h, detects supernatant with silver nitrate Liquid shows non-sodium chloride, and centrifugation obtains starch, is dried in vacuo at 45 DEG C, and water content controls below 15%.
The converted starch composition of above-mentioned preparation is applied in preparing cosmetics, cosmetics described in the present embodiment are to dispel Acne lotion.
A kind of anti-acne lotion includes the ingredient of following mass percent: 2% converted starch composition described above, 0.2% Soluble polysaccharide, 0.1% Flos Nelumbinis extract, 0.1% grape seed extract, 4% saualane, 8% coixenolide, 1% palmitinic acid second The own ester of base, 2% butanediol, 2% propylene glycol, 2% collagen, 0.2% Sodium Hyaluronate, 1% glycerol, 0.001% carbomer, 0.1% triethanolamine, 0.01% essence, 77.289% deionized water.
Embodiment 2
A kind of converted starch composition is mixed by cornstarch, cordate houttuynia starch and tapioca according to the ratio of 3:1:8, After the cornstarch, cordate houttuynia starch and tapioca are proportionally mixed, slurries are modulated into, 0.03% hydrochloric acid is added PH to 7.0 is adjusted, slurries are added to 5% carragheen or guar gum for accounting for the converted starch composition being sufficiently swollen, are stirred Mix 30min, form mixed liquor, mixed liquor is placed in baking oven and is dried, until moisture content be down to 10% hereinafter, after in temperature be 65 Xeothermic reaction obtains converted starch composition at DEG C.
And converted starch composition is high amylose starch, the molecular weight of the high amylose starch is 100000~999999, purity 93.1%.
Wherein, the preparation of the cordate houttuynia starch are as follows: the snow-white rhizome of selected color removes root, be put into beater into The sodium chloride solution that the concentration of 3 times of amounts is 5% is added in row mashing, and filtering takes filtrate, stands 3h, detects supernatant with silver nitrate Liquid shows non-sodium chloride, and centrifugation obtains starch, is dried in vacuo at 50 DEG C, and water content controls below 15%.
The converted starch composition of above-mentioned preparation is applied in preparing cosmetics, cosmetics described in the present embodiment are to dispel Acne lotion.
A kind of anti-acne lotion includes the ingredient of following mass percent: 10% converted starch composition described above, 1% white Art polysaccharide, 0.1% Flos Nelumbinis extract, 0.5% grape seed extract, 10% saualane, 10% coixenolide, 5% palmitinic acid second The own ester of base, 8% butanediol, 6% propylene glycol, 4% collagen, 2.0% Sodium Hyaluronate, 3% glycerol, 0.003% carbomer, 0.1% triethanolamine, 0.01% essence, 40.26% deionized water.
Embodiment 3
A kind of converted starch composition is mixed by cornstarch, cordate houttuynia starch and tapioca according to the ratio of 3:5:1, After the cornstarch, cordate houttuynia starch and tapioca are proportionally mixed, slurries are modulated into, 0.02% hydrochloric acid is added PH to 7.0 is adjusted, slurries are added to 4% carragheen or guar gum for accounting for the converted starch composition being sufficiently swollen, are stirred Mix 25min, form mixed liquor, mixed liquor is placed in baking oven and is dried, until moisture content be down to 10% hereinafter, after in temperature be 50 Xeothermic reaction obtains converted starch composition at DEG C.
And converted starch composition is high amylose starch, the molecular weight of the high amylose starch is 100000~999999, purity 95.1%.
Wherein, the preparation of the cordate houttuynia starch are as follows: the snow-white rhizome of selected color removes root, be put into beater into The sodium chloride solution that the concentration of 2 times of amounts is 5% is added in row mashing, and filtering takes filtrate, stands 3h, detects supernatant with silver nitrate Liquid shows non-sodium chloride, and centrifugation obtains starch, is dried in vacuo at 50 DEG C, and water content controls below 15%.
The converted starch composition of above-mentioned preparation is applied in preparing cosmetics, cosmetics described in the present embodiment are to dispel Acne lotion.
A kind of anti-acne lotion includes the ingredient of following mass percent: 10% converted starch composition described above, 0.5% Soluble polysaccharide, 0.4% Flos Nelumbinis extract, 0.5% grape seed extract, 12% saualane, 12% coixenolide, 3% palmitinic acid Ethylhexyl, 8% butanediol, 12% propylene glycol, 8% collagen, 1.2% Sodium Hyaluronate, 2% glycerol, 0.002% card wave Nurse, 0.2% triethanolamine, 0.02% essence, 30.16% deionized water.
Embodiment 4
A kind of converted starch composition is mixed by cornstarch, cordate houttuynia starch and tapioca according to the ratio of 3:3:4, After the cornstarch, cordate houttuynia starch and tapioca are proportionally mixed, slurries are modulated into, 0.03% hydrochloric acid is added PH to 6.5 is adjusted, slurries are added to 4% carragheen or guar gum for accounting for the converted starch composition being sufficiently swollen, are stirred Mix 25min, form mixed liquor, mixed liquor is placed in baking oven and is dried, until moisture content be down to 10% hereinafter, after in temperature be 60 Xeothermic reaction obtains converted starch composition at DEG C.
And converted starch composition is high amylose starch, the molecular weight of the high amylose starch is 100000~999999, purity 95.6%.
Wherein, the preparation of the cordate houttuynia starch are as follows: the snow-white rhizome of selected color removes root, be put into beater into The sodium chloride solution that the concentration of 3 times of amounts is 5% is added in row mashing, and filtering takes filtrate, stands 3h, detects supernatant with silver nitrate Liquid shows non-sodium chloride, and centrifugation obtains starch, is dried in vacuo at 50 DEG C, and water content controls below 15%.
The converted starch composition of above-mentioned preparation is applied in preparing cosmetics, cosmetics described in the present embodiment are to dispel Acne lotion.
A kind of anti-acne lotion includes the ingredient of following mass percent: 8% converted starch composition described above, 0.2% Soluble polysaccharide, 0.1% Flos Nelumbinis extract, 0.4% grape seed extract, 8% saualane, 12% coixenolide, 4% palmitinic acid second The own ester of base, 8% butanediol, 10% propylene glycol, 8% collagen, 1.2% Sodium Hyaluronate, 2% glycerol, 0.002% card wave Nurse, 0.2% triethanolamine, 0.01% essence, 37.888% deionized water.
Comparative example 1
A kind of converted starch composition is mixed by cornstarch, tapioca according to the ratio of 3:1, and the corn is formed sediment After powder, tapioca proportionally mix, slurries are modulated into, 0.02% salt acid for adjusting pH is added to 7.0, slurries addition is filled Divide 4% carragheen or guar gum for accounting for the converted starch composition of swelling, stir 25min, forms mixed liquor, will mix Liquid is placed in baking oven and dries, until moisture content be down to 10% hereinafter, after temperature be 50 DEG C at it is xeothermic reaction i.e. obtain denaturation shallow lake Compound powder.
And converted starch composition is high amylose starch, the molecular weight of the high amylose starch is 100000~999999, purity 96.5%.
The converted starch composition of above-mentioned preparation is applied in preparing cosmetics, cosmetics described in the present embodiment are to dispel Acne lotion.
A kind of anti-acne lotion includes the ingredient of following mass percent: 10% converted starch composition described above, 0.5% Soluble polysaccharide, 0.4% Flos Nelumbinis extract, 0.5% grape seed extract, 12% saualane, 12% coixenolide, 3% palmitinic acid Ethylhexyl, 8% butanediol, 12% propylene glycol, 8% collagen, 1.2% Sodium Hyaluronate, 2% glycerol, 0.002% card wave Nurse, 0.2% triethanolamine, 0.02% essence, 30.16% deionized water.
Comparative example 2
A kind of converted starch composition is mixed by cornstarch, cordate houttuynia starch and tapioca according to the ratio of 3:5:1, After the cornstarch, cordate houttuynia starch and tapioca are proportionally mixed, slurries are modulated into, starch composition is dried to obtain Object, wherein the preparation of the cordate houttuynia starch are as follows: the snow-white rhizome of selected color removes root, is put into beater and beaten The sodium chloride solution that the concentration of 2 times of amounts is 5% is added in slurry, and filtering takes filtrate, stands 3h, detects supernatant with silver nitrate, shows Show non-sodium chloride, be centrifuged, obtain starch, be dried in vacuo at 50 DEG C, water content controls below 15%.
The starch composites of above-mentioned preparation are applied in preparing cosmetics, cosmetics described in the present embodiment are anti-acne Liquid.
A kind of anti-acne lotion includes the ingredient of following mass percent: 10% above-mentioned starch composites, 0.5% Rhizoma Atractylodis Macrocephalae are more Sugar, 0.4% Flos Nelumbinis extract, 0.5% grape seed extract, 12% saualane, 12% coixenolide, 3% palm acid ethyl oneself Ester, 8% butanediol, 12% propylene glycol, 8% collagen, 1.2% Sodium Hyaluronate, 2% glycerol, 0.002% carbomer, 0.2% triethanolamine, 0.02% essence, 30.16% deionized water.
Comparative example 3
A kind of converted starch composition is mixed by cornstarch, cordate houttuynia starch and tapioca according to the ratio of 3:5:1, After the cornstarch, cordate houttuynia starch and tapioca are proportionally mixed, slurries are modulated into, 0.02% hydrochloric acid is added PH to 7.0 is adjusted, slurries are added to 4% carragheen or guar gum for accounting for the converted starch composition being sufficiently swollen, are stirred Mix 25min, form mixed liquor, mixed liquor is placed in baking oven and is dried, until moisture content be down to 10% hereinafter, after in temperature be 50 Xeothermic reaction obtains converted starch composition at DEG C.
And converted starch composition is high amylose starch, the molecular weight of the high amylose starch is 100000~999999, purity 94.8%.
Wherein, the preparation of the cordate houttuynia starch are as follows: the snow-white rhizome of selected color removes root, be put into beater into The sodium chloride solution that the concentration of 2 times of amounts is 5% is added in row mashing, and filtering takes filtrate, stands 3h, detects supernatant with silver nitrate Liquid shows non-sodium chloride, and centrifugation obtains starch, is dried in vacuo at 50 DEG C, and water content controls below 15%.
The converted starch composition of above-mentioned preparation is applied in preparing cosmetics, cosmetics described in the present embodiment are to dispel Acne lotion.
A kind of anti-acne lotion includes the ingredient of following mass percent: 0.5% soluble polysaccharide, 0.4% Flos Nelumbinis extract, 0.5% grape seed extract, 12% saualane, 12% coixenolide, 3% ethylhexyl palmitate, 8% butanediol, 12% the third Glycol, 8% collagen, 1.2% Sodium Hyaluronate, 2% glycerol, 0.002% carbomer, 0.2% triethanolamine, 0.02% perfume Essence, 40.16% deionized water.
Comparative example 4
A kind of converted starch composition is mixed by cornstarch, cordate houttuynia starch and tapioca according to the ratio of 8:1:15 It closes, after the cornstarch, cordate houttuynia starch and tapioca are proportionally mixed, is modulated into slurries, is added 0.05% 5% carragheen or Guar for accounting for the converted starch composition being sufficiently swollen is added to 7.0 in slurries by salt acid for adjusting pH Glue, stir 30min, formed mixed liquor, mixed liquor is placed in baking oven and is dried, until moisture content be down to 10% hereinafter, after in temperature Degree is that xeothermic reaction obtains converted starch composition at 65 DEG C.
And converted starch composition is high amylose starch, the molecular weight of the high amylose starch is 100000~999999, purity 92.5%.
Wherein, the preparation of the cordate houttuynia starch are as follows: the snow-white rhizome of selected color removes root, be put into beater into The sodium chloride solution that the concentration of 5 times of amounts is 5% is added in row mashing, and filtering takes filtrate, stands 1~3h, is detected with silver nitrate Clear liquid shows non-sodium chloride, and centrifugation obtains starch, is dried in vacuo at 50 DEG C, and water content controls below 15%.
The converted starch composition of above-mentioned preparation is applied in preparing cosmetics, cosmetics described in the present embodiment are to dispel Acne lotion.
A kind of anti-acne lotion includes the ingredient of following mass percent: 15% converted starch composition described above, 1% white Art polysaccharide, 0.9% Flos Nelumbinis extract, 0.5% grape seed extract, 16% saualane, 15% coixenolide, 1% palmitinic acid second The own ester of base, 2% butanediol, 2% propylene glycol, 2% collagen, 0.2% Sodium Hyaluronate, 1% glycerol, 0.001% carbomer, 0.1% triethanolamine, 0.01% essence, 43.289% deionized water.
Anti-inflammatory efficacy verifying
Main material: mouse macrophage RAW264.7 is (thin from Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Born of the same parents resource center), anti-acne lotion, the mouse prostate element E2 (PGE2) of the anti-acne lotion of Examples 1 to 4 and comparative example 1~4 ELISA detection kit, murine tumor necrosis factor-α (TNF-α) ELISA detection kit, lipopolysaccharides (LPS).
Method: (1) influence grown with mtt assay detection anti-acne lotion to RAW264.7 cell adjusts RAW264.7 cell Density is inoculated in 96 porocyte culture plates, every 1000 μ L of hole to 5x104/mL, is placed in 37 DEG C, in 5%CO2 cell incubator Culture is for 24 hours.The culture medium in cell plates is sucked out after culture, it is 500 200 μ of μ g/mL biology that concentration is separately added into processing group Isometric serum free medium is added in the L dilution of lotion containing anti-acne, blank control group, continues to cultivate for 24 hours, every group of 3 multiple holes. After for 24 hours, the MTT solution of every group of 20 μ L of addition continues after cultivating 4h, takes out 96 orifice plates, liquid in hole is sucked out, is added 150 μ L's Absorbance (A) value is measured at 490nm after DMSO, concussion 5min, calculates the survival rate of cell.Survival rate=medicine group average A-value/ Blank control group average A-value.
(2) influence of PGE2, respectively control group (serum-free training are discharged to lipopolysaccharides (LPS) stimulation RAW264.7 cell Support base), Examples 1 to 4 distinguishes administration group (LPS of the anti-acne lotion of 100 μ g/mL and 1 μ g/mL), 1~4 group of (100 μ of comparative example The LPS of the comparative example of g/mL and 1 μ g/mL), cell culture and method and (1) together, collect cell conditioned medium after LPS stimulates 18h Liquid requires to carry out PGE2 detection according to ELISA kit specification, calculates inhibiting rate, be denoted as inhibiting rate 1.
(3) influence of TNF-a, respectively control group (serum free medium) are discharged to LPS stimulation RAW264.7 cell, it is real It applies example 1~4 and distinguishes administration group (LPS of the anti-acne lotion of 100 μ g/mL and 1 μ g/mL), (100 μ g/mL's dispels Examples 1 to 4 group The LPS of acne lotion and 1 μ g/mL), LPS collects cell conditioned medium after stimulating 18h, carries out by the operation requirement of ELISA kit specification TNF-a detection, calculates inhibiting rate, is denoted as inhibiting rate 2, the results are shown in Table 1.
Table 1
By the data analysis of survival rate in table 1 it is found that the anti-acne lotion acellular poison side effect of preparation, ingredient is mildly, no Stimulation;It analyzes from the data of inhibiting rate 1 and inhibiting rate 2 it is found that illustrating to apply converted starch composition and is preparing anti-acne It in liquid, can effectively inhibit the release of the cellular inflammation factor, assign the good anti-inflammatory efficacy of cosmetics.
Bacteriostasis efficacy verifying
For trying strain: staphylococcus aureus, Escherichia coli, propionibacterium acnes
Culture medium and condition of culture: ordinary nutrient agar culture medium (37 DEG C, 48h culture, for staphylococcus aureus, Escherichia coli culture), cooked meat medium (be used for propionibacterium acnes culture).
Test method: referring to National Standard of the People's Republic of China GB15797-2002, " Disposable Sanitary Accessory is defended Appendix C 4 " stripping property resists (suppression) bacterium product bacteriostasis property test method " in raw standard ".
Operating procedure:
(1) test organisms for 24 hours washed down with 0.03mol/LPBS by culture, is made 106Cfu/mL bacteria suspension.
(2) above-mentioned bacteria suspension 0.1mL even spread mixing is added in each 1g of the anti-acne lotion of Example 3 and comparative example 3, opens After beginning timing 20min, 9mL0.03mol/LPBS is added and mixes well, after making appropriate dilution, takes wherein 2-3 dilution, respectively It takes 1mL to be placed in two parallel plates, adds corresponding culture medium difference Hybrid NC machine tool.Make colony count.The above test repeats 3 It is secondary, its average is taken, while setting blank control.
It calculates: X=(A-B)/A × 100%
In formula: X --- bacteriostasis rate, %
A --- control sample averagely recycles bacterium colony;
B --- test specimen averagely recycles bacterium colony.
Bacteriostasis efficacy evaluation: efficacy assessments divide three-level: it is effective, effective and invalid, such as the following table 2.
Table 2
According to above-mentioned test method, the bacteriostatic test of 20min is carried out, the results are shown in Table 3.
Table 3
By the data analysis of table 3 it is found that converted starch composition of the invention is applied in preparation anti-acne lotion with aobvious The fungistatic effect of work prevents microbial reproduction and secretion harmful substance, causes the generation and deterioration of cellular inflammation.
In other test, the anti-acne lotion that will be prepared in embodiment 3 and comparative example 3 is heated to 45 DEG C, shakes 2h Afterwards, it places, observes the stability of anti-acne emulsion system, the anti-acne lotion obtained in comparative example 3 is placing 12d, occurs light Micro- lamination, and the product integral system that embodiment 3 is prepared is stablized, and illustrates converted starch composition in cosmetics It plays a role in system.
Above the embodiments of the present invention are described in detail, but the present invention is not limited to described embodiments.It is right For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out more Kind change, modification, replacement and modification, still fall in protection scope of the present invention.

Claims (10)

1. a kind of converted starch composition, it is characterised in that: by cornstarch, cordate houttuynia starch and tapioca according to 1~3:1 The ratio of~5:1~8 mixes, the converted starch composition obtained after denaturing step;And converted starch composition is high score Son amount amylose, the molecular weight of the high amylose starch are 100000~999999, and purity is 92~100%.
2. converted starch composition according to claim 1, it is characterised in that: the preparation method of the cordate houttuynia starch Are as follows: the snow-white cordate houttuynia rhizome of selected color removes root, is put into beater and is beaten, and the concentration that 1~3 times of amount is added is 5% sodium chloride solution, filtering take filtrate, stand 1~3h, detect supernatant with silver nitrate, show non-sodium chloride, and centrifugation obtains It is dried in vacuo to starch, then at 45~50 DEG C, water content controls below 15%.
3. converted starch composition according to claim 1, it is characterised in that: the denaturing step are as follows: by the corn Starch, cordate houttuynia starch and tapioca proportionally mix, and are modulated into slurries, and 0.01~0.03% salt acid for adjusting pH is added To 6.0~7.0, slurries are added in the ion sol solution being sufficiently swollen, stirs evenly to form mixed liquor, mixed liquor is placed in baking It is dried in case, until moisture content, which is down to 10%, obtains converted starch composition hereinafter, then carrying out xeothermic reaction.
4. converted starch composition according to claim 3, it is characterised in that: the content of the ion glue accounts for the denaturation The 3~5% of starch composites.
5. converted starch composition according to claim 3, it is characterised in that: the ion glue is carragheen or Guar Glue.
6. converted starch composition according to claim 3, it is characterised in that: the mixing time is 20~30min.
7. converted starch composition according to claim 3, it is characterised in that: the temperature of the xeothermic reaction is 45~65 ℃。
8. a kind of converted starch composition as claimed in any one of claims 1 to 3 is preparing the application in cosmetics.
9. application according to claim 8, it is characterised in that: the cosmetics are anti-acne lotion.
10. a kind of anti-acne lotion, it is characterised in that: the ingredient including following mass percent: 2~10% such as claim 1 institutes Converted starch composition, 0.2~1% soluble polysaccharide, 0.1~0.9% Flos Nelumbinis extract, 0.1~0.5% grape pip is stated to extract Object, 4~16% saualanes, 8~15% coixenolides, 1~5% ethylhexyl palmitate, 2~10% butanediols, 2~15% the third Glycol, 2~10% collagens, 0.2~2.0% Sodium Hyaluronate, 1~3% glycerol, 0.001~0.003% carbomer, 0.1 ~0.3% triethanolamine, 0.01~0.03% essence, deionized water surplus.
CN201910425429.8A 2019-05-21 2019-05-21 A kind of converted starch composition and its application Withdrawn CN110141530A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113214544A (en) * 2020-11-26 2021-08-06 吴秀连 Concentrated alkali wet method modified starch composition and application

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113214544A (en) * 2020-11-26 2021-08-06 吴秀连 Concentrated alkali wet method modified starch composition and application

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