CN110140659B - Breeding and planting method for tissue culture of bitter ropes - Google Patents
Breeding and planting method for tissue culture of bitter ropes Download PDFInfo
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- CN110140659B CN110140659B CN201910600827.9A CN201910600827A CN110140659B CN 110140659 B CN110140659 B CN 110140659B CN 201910600827 A CN201910600827 A CN 201910600827A CN 110140659 B CN110140659 B CN 110140659B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a breeding and planting method for tape tissue culture, which comprises the following steps of 1) recovering the overground part of tape, and removing leaves to obtain a sterile material; 2) inoculating the sterile material into 1/2MS + IAA 0.2mg/L culture medium; 3) cutting off stems of the aseptic materials with buds growing in the step 2), and inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5 mg/L; 4) cutting the differentiated cluster buds in the step 3) into stem sections with terminal buds or 1-2 axillary buds, inoculating the stem sections on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture, and obtaining rooting test-tube plantlets; 5) the rooting test-tube plantlet is transplanted to a growth substrate, the seedling growing method is simple to operate and low in cost, and a technical foundation is laid for rapid propagation of the bitter ropes.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine planting, in particular to a breeding and planting method for tissue culture of a bitter rope.
Background
Tape (dreg sine) alias: milk pulp vine, caulis akebiae, white pulp vine, white pulp grass, Tongguan powder, Chinese tulip, Chinese south mountain vine and wild steeping Tong; belongs to the plant of south mountain cane of asclepiadaceae, which is a special plant of China. The cultivation method is distributed in Guangxi, Shaanxi, Guizhou, Sichuan, Zhejiang, Yunnan, Hubei, Jiangsu, Gansu and other places in continental China, grows in an area with an altitude of 500-3,000 meters, generally grows in sparse forests or shrubs in mountainous regions, and is not artificially introduced and cultivated at present.
Climbing wood vines; the stem has a skin hole; the young branches have brown villi. The leaf paper is in an oval heart shape or a nearly round shape, the base part is in a heart shape, the length is 5-11 cm, the width is 4-6 cm, the leaf surface is short and soft, the leaf surface is old and gradually unhaired, and the leaf back is villi; about 5 lateral veins on each side; the petiole is 1.5-4 cm long, villous, and has clumpy glands at the top. The umbellate parasporal inflorescence is axillary, and the number of flowers is as many as 20; the sepal is oval to oval, and the base of the inner surface is provided with glands; the corolla is in spoke shape, the diameter is 1.6 cm, the outer surface is white, the inner surface is purple red, the corolla is oval, the length is 6-7 mm, the width is 4-6 mm, the top end is blunt and slightly concave, and the peripheral hair is arranged.
The internal form of the assistant corolla splits and has swollen flesh and sharp internal angle at the end; the top of the anther is provided with a membrane; the pollen block is long round and upright; the ovary has no hair, the carpel is separated from the skin, the stigma is conical, the base is pentagonal, and the top end is 2 fissures. The whole-length fruit from the Gu is needle-shaped, 5-6 cm long, about 1.2 cm in diameter, corrugated on the outer fruit skin, short and soft; the seeds are flat, oval and long, 9 mm long, 5 mm wide and 2cm long at the top. The flowering period is 4-8 months, and the fruit period is 7-10 months.
Disclosure of Invention
The invention provides a breeding and planting method for tissue culture of a bitter rope.
The scheme of the invention is as follows:
a breeding and planting method for tissue culture of a bitter rope comprises the following steps:
1) the method comprises the following steps of (1) recovering the overground part of a tape, removing leaves, cutting off the upper half part with more growing points, carrying out oscillation washing for 18-22 min by using sterile water, carrying out oscillation washing for 2-3 min by using 0.05% detergent, rinsing the tape to be free of foam by using sterile water, adding 70-75% of ethanol for 10-20 seconds for sterilization, carrying out three times of sterilization by using sterile water, adding 0.05% of mercuric chloride solution for oscillation sterilization for 1-3 min, adding sterile water with the same volume, continuing oscillation sterilization for 12-15 min, and carrying out five times of sterilization by using sterile water to obtain a sterile material;
2) inoculating the sterile material into a culture medium 1/2MS + IAA 0.2mg/L, adding sucrose 15g/L, adjusting the pH to 5.8-6.0, illuminating at 2500lx at 22-24 ℃ for 12 h/d; obtaining a grown sterile material;
3) cutting off stems of the aseptic materials with buds growing in the step 2), inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5mg/L, and carrying out bud differentiation culture under the illumination condition to obtain differentiated cluster buds;
4) cutting the differentiated cluster buds in the step 3) into stem sections with terminal buds or 1-2 axillary buds, inoculating the stem sections on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture, and obtaining rooting test-tube plantlets;
5) transplanting the rooting test-tube plantlets to a growth substrate, culturing for thirty days under the conditions that the temperature is 20-27 ℃, the humidity is 75-90% and no direct light exists, transplanting the obtained test-tube plantlets to a hillside-shaped nursery garden at the end of four months and in the last ten days of May, and building frames when the test-tube plantlets of the nursery garden grow to 28-32 cm.
As a preferable technical scheme, the growth substrate comprises furnace slag, humus, plant ash, sterilized chicken manure fermented for more than half a year, activated carbon and cane sugar.
As a preferable technical scheme, the feeding ratio of the furnace ash, the humus, the plant ash, the sterilized chicken manure fermented for more than half a year, the activated carbon and the cane sugar is 1:1:1:0.5:0.02:0.3, and the materials are subjected to high-pressure high-temperature sterilization treatment and deinsectization treatment and then are uniformly stirred at normal temperature.
And as a preferred technical scheme, when the rooting test-tube plantlet in the step 5) grows for more than 3cm, spraying nutrient water every 7-10 days, and keeping ventilation for 2-5 hours.
As a preferred technical scheme, the sterilized chicken manure, the monopotassium phosphate, the biological chitin and the water are fermented for more than half a year.
As a preferred technical scheme, the feed ratio of the sterilized chicken manure fermented for more than half a year, the monopotassium phosphate, the biological chitin to the water is 1: 0.05: 2.2:10.
Preferably, the growth substrate in step 5) is in a state that the growth substrate can be grabbed by hands, can be kneaded into a cluster, and can be loosened after the hands are loosened.
As a preferable technical scheme, the water content of the soil in the nursery garden in the step 5) is 14-18%.
Due to the adoption of the technical scheme, the breeding and planting method for the tissue culture of the bitter ropes comprises the steps of 1) recovering the overground part of the bitter ropes, removing leaves, cutting off the upper half part with more growing points, carrying out oscillation washing for 18-22 min by using sterile water, carrying out oscillation washing for 2-3 min by using 0.05% detergent, rinsing the bitter ropes by using the sterile water until no foam exists, then adding 70-75% of ethanol for 10-20 seconds for sterilization, carrying out three times of sterile washing, adding 0.05% of mercuric chloride solution for oscillation sterilization for 1-3 min, adding the sterile water with the same volume, continuing oscillation sterilization for 12-15 min, and carrying out five times of sterile washing to obtain the sterile material; 2) inoculating the sterile material into a culture medium 1/2MS + IAA 0.2mg/L, adding sucrose 15g/L, adjusting the pH to 5.8-6.0, illuminating at 2500lx at 22-24 ℃ for 12 h/d; obtaining a grown sterile material; 3) cutting off stems of the aseptic materials with buds growing in the step 2), inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5mg/L, and carrying out bud differentiation culture under the illumination condition to obtain differentiated cluster buds; 4) cutting the differentiated cluster buds in the step 3) into stem sections with terminal buds or 1-2 axillary buds, inoculating the stem sections on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture, and obtaining rooting test-tube plantlets; 5) transplanting the rooting test-tube plantlets to a growth substrate, culturing for thirty days under the conditions that the temperature is 20-27 ℃, the humidity is 75-90% and no direct light exists, transplanting the obtained test-tube plantlets to a hillside-shaped nursery garden at the end of four months and in the last ten days of May, and building frames when the test-tube plantlets of the nursery garden grow to 28-32 cm.
The invention has the advantages that:
the invention carries out tissue culture through the bitter ropes, which shows that the tissue culture of the bitter ropes is realized, the rooting rate induced by the method reaches 100 percent, the survival rate of seedlings reaches 99 percent, the occurrence rate of invalid seedlings is extremely low, the seedlings can grow normally and vigorously, the plant bodies are thicker, and the survival rate after transplantation also reaches 99 percent.
Detailed Description
In order to make up for the above deficiencies, the invention provides a breeding and planting method for tissue culture of a bitter rope to solve the problems in the background technology.
A breeding and planting method for tissue culture of a bitter rope comprises the following steps:
1) the method comprises the following steps of (1) recovering the overground part of a tape, removing leaves, cutting off the upper half part with more growing points, carrying out oscillation washing for 18-22 min by using sterile water, carrying out oscillation washing for 2-3 min by using 0.05% detergent, rinsing the tape to be free of foam by using sterile water, adding 70-75% of ethanol for 10-20 seconds for sterilization, carrying out three times of sterilization by using sterile water, adding 0.05% of mercuric chloride solution for oscillation sterilization for 1-3 min, adding sterile water with the same volume, continuing oscillation sterilization for 12-15 min, and carrying out five times of sterilization by using sterile water to obtain a sterile material;
2) inoculating the sterile material into a culture medium 1/2MS + IAA 0.2mg/L, adding sucrose 15g/L, adjusting the pH to 5.8-6.0, illuminating at 2500lx at 22-24 ℃ for 12 h/d; obtaining a grown sterile material;
3) cutting off stems of the aseptic materials with buds growing in the step 2), inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5mg/L, and carrying out bud differentiation culture under the illumination condition to obtain differentiated cluster buds;
4) cutting the differentiated cluster buds in the step 3) into stem sections with terminal buds or 1-2 axillary buds, inoculating the stem sections on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture, and obtaining rooting test-tube plantlets;
5) transplanting the rooting test-tube plantlets to a growth substrate, culturing for thirty days under the conditions that the temperature is 20-27 ℃, the humidity is 75-90% and no direct light exists, transplanting the obtained test-tube plantlets to a hillside-shaped nursery garden at the end of four months and in the last ten days of May, and building frames when the test-tube plantlets of the nursery garden grow to 28-32 cm.
The growth substrate comprises furnace ash, humus, plant ash, sterilized chicken manure fermented for more than half a year, activated carbon and sucrose.
The feeding ratio of the furnace ash, humus soil, plant ash, sterilized chicken manure fermented for more than half a year, active carbon and cane sugar is 1:1:1:0.5:0.02:0.3, and the raw materials are subjected to high-pressure high-temperature sterilization treatment and deinsectization treatment and then are uniformly stirred at normal temperature.
And 5) spraying nutrient water every 7-10 days when the rooting test-tube plantlet grows for more than 3cm, and keeping ventilation for 2-5 hours.
The sterilized chicken manure fermented for more than half a year, monopotassium phosphate, biological chitin and water.
The feed ratio of the sterilized chicken manure fermented for more than half a year, the monopotassium phosphate, the biological chitin to the water is 1: 0.05: 2.2:10.
The growth substrate in the step 5) is kept in a state that the growth substrate can be grabbed by hands, can be kneaded into a cluster and can be dispersed after the hands are loosened.
The water content of the soil in the nursery garden in the step 5) is 14-18%.
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1:
a breeding and planting method for tissue culture of a bitter rope comprises the following steps:
1) the overground part of the tape is picked back, the leaves are removed, the upper half part with more growing points is cut off, then the vibrating washing is carried out for 18min by using sterile water, then the vibrating washing is carried out for 2min by using 0.05 percent of detergent, then the rinsing is carried out to no foam by using the sterile water, then 70 percent of ethanol is added for 10 seconds for sterilization, the three times of the sterilizing washing are carried out by using the sterile water, 0.05 percent of mercuric chloride solution is added for vibrating sterilization for 1min, then the sterile water with the same volume is added, the vibrating sterilization is continued for 12min, and then the washing is carried out for five times by using the sterile water, thus obtaining the sterile material;
2) inoculating the sterile material into 1/2MS + IAA 0.2mg/L culture medium, adding sucrose 15g/L, pH5.8, irradiating with 2500lx light at 22 deg.C for 12 h/d; obtaining a grown sterile material;
3) cutting off stems of the aseptic materials with buds growing in the step 2), inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5mg/L, and carrying out bud differentiation culture under the illumination condition to obtain differentiated cluster buds;
4) cutting the differentiated cluster buds in the step 3) into stem segments with terminal buds or 1 axillary bud, inoculating the stem segments on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture to obtain rooted test-tube plantlets;
5) transplanting the rooted test-tube plantlets onto a growth substrate, culturing at 20 ℃ and 75% humidity without direct light, culturing for thirty days, transplanting the obtained test-tube plantlets into a hillside nursery at the end of the fourth month and the last ten days of May, and building frames when the test-tube plantlets of the nursery grow to 28 cm.
The growth substrate comprises furnace ash, humus, plant ash, sterilized chicken manure fermented for more than half a year, activated carbon and sucrose.
The feeding ratio of the furnace ash, humus soil, plant ash, sterilized chicken manure fermented for more than half a year, active carbon and cane sugar is 1:1:1:0.5:0.02:0.3, and the raw materials are subjected to high-pressure high-temperature sterilization treatment and deinsectization treatment and then are uniformly stirred at normal temperature.
And 5) spraying nutrient water every 7 days when the rooting test-tube plantlet grows for more than 3cm, and keeping ventilation for 2 hours.
The sterilized chicken manure fermented for more than half a year, monopotassium phosphate, biological chitin and water.
The feed ratio of the sterilized chicken manure fermented for more than half a year, the monopotassium phosphate, the biological chitin to the water is 1: 0.05: 2.2:10.
The growth substrate in the step 5) is kept in a state that the growth substrate can be grabbed by hands, can be kneaded into a cluster and can be dispersed after the hands are loosened.
The water content of the soil in the nursery in the step 5) is 14%.
Example 2:
a breeding and planting method for tissue culture of a bitter rope comprises the following steps:
1) the overground part of the tape is picked back, the leaves are removed, the upper half part with more growing points is cut off, then the vibrating washing is carried out for 22min by using sterile water, then the vibrating washing is carried out for 3min by using 0.05 percent of detergent, then the rinsing is carried out by using the sterile water until no foam exists, then 75 percent of ethanol is added for 20 seconds for sterilization, the three times of the sterilizing washing are carried out by using the sterile water, 0.05 percent of mercuric chloride solution is added for vibrating sterilization for 3min, the equal volume of the sterile water is added into the solution, the vibrating sterilization is continued for 15min, and then the sterilizing water is washed for five times by using the sterile water, thus obtaining the sterile material;
2) inoculating the sterile material into 1/2MS + IAA 0.2mg/L culture medium, adding sucrose 15g/L, pH6.0, irradiating with 2500lx light at 24 deg.C for 12 h/d; obtaining a grown sterile material;
3) cutting off stems of the aseptic materials with buds growing in the step 2), inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5mg/L, and carrying out bud differentiation culture under the illumination condition to obtain differentiated cluster buds;
4) cutting the differentiated cluster buds in the step 3) into stem sections with terminal buds or 2 axillary buds, inoculating the stem sections on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture to obtain rooting test-tube plantlets;
5) transplanting the rooted test-tube plantlets onto a growth substrate, culturing at 27 ℃ and 90% humidity without direct light, culturing for thirty days, transplanting the obtained test-tube plantlets into a hilly nursery garden at the end of the fourth month and the last ten days of May, and building frames when the test-tube plantlets of the nursery garden grow to 32 cm.
The growth substrate comprises furnace ash, humus, plant ash, sterilized chicken manure fermented for more than half a year, activated carbon and sucrose.
The feeding ratio of the furnace ash, humus soil, plant ash, sterilized chicken manure fermented for more than half a year, active carbon and cane sugar is 1:1:1:0.5:0.02:0.3, and the raw materials are subjected to high-pressure high-temperature sterilization treatment and deinsectization treatment and then are uniformly stirred at normal temperature.
And 5) spraying nutrient water every 10 days when the rooting test-tube plantlet grows for more than 3cm, and keeping ventilation for 2-5 h.
The sterilized chicken manure fermented for more than half a year, monopotassium phosphate, biological chitin and water.
The feed ratio of the sterilized chicken manure fermented for more than half a year, the monopotassium phosphate, the biological chitin to the water is 1: 0.05: 2.2:10.
The growth substrate in the step 5) is kept in a state that the growth substrate can be grabbed by hands, can be kneaded into a cluster and can be dispersed after the hands are loosened.
The water content of the soil in the nursery in the step 5) is 18%.
Example 3:
a breeding and planting method for tissue culture of a bitter rope comprises the following steps:
1) the overground part of the tape is picked back, the leaves are removed, the upper half part with more growing points is cut off, then the vibrating washing is carried out for 20min by using sterile water, then the vibrating washing is carried out for 3min by using 0.05 percent of detergent, then the rinsing is carried out to no foam by using the sterile water, then 73 percent of ethanol is added for 15 seconds for sterilization, the three times of the sterilizing washing are carried out by using the sterile water, 0.05 percent of mercuric chloride solution is added for vibrating sterilization for 2min, then the sterile water with the same volume is added, the vibrating sterilization is continued for 14min, and then the washing is carried out for five times by using the sterile water, thus obtaining the sterile material;
2) inoculating the sterile material into 1/2MS + IAA 0.2mg/L culture medium, adding sucrose 15g/L, pH6.0, irradiating with 2500lx light at 22 deg.C for 12 h/d; obtaining a grown sterile material;
3) cutting off stems of the aseptic materials with buds growing in the step 2), inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5mg/L, and carrying out bud differentiation culture under the illumination condition to obtain differentiated cluster buds;
4) cutting the differentiated cluster buds in the step 3) into stem sections with terminal buds or 2 axillary buds, inoculating the stem sections on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture to obtain rooting test-tube plantlets;
5) transplanting the rooted test-tube plantlet to a growth substrate, culturing at 24 ℃ and 80% humidity without direct light, culturing for thirty days, transplanting the obtained test-tube plantlet to a hillside-shaped nursery garden at the end of the fourth month and the last ten days of May, and building frames when the test-tube plantlet of the nursery garden grows to 30 cm.
The growth substrate comprises furnace ash, humus, plant ash, sterilized chicken manure fermented for more than half a year, activated carbon and sucrose.
The feeding ratio of the furnace ash, humus soil, plant ash, sterilized chicken manure fermented for more than half a year, active carbon and cane sugar is 1:1:1:0.5:0.02:0.3, and the raw materials are subjected to high-pressure high-temperature sterilization treatment and deinsectization treatment and then are uniformly stirred at normal temperature.
And 5) spraying nutrient water every 8 days when the rooting test-tube plantlet grows for more than 3cm, and keeping ventilation for 3 hours.
The sterilized chicken manure fermented for more than half a year, monopotassium phosphate, biological chitin and water.
The feed ratio of the sterilized chicken manure fermented for more than half a year, the monopotassium phosphate, the biological chitin to the water is 1: 0.05: 2.2:10.
The growth substrate in the step 5) is kept in a state that the growth substrate can be grabbed by hands, can be kneaded into a cluster and can be dispersed after the hands are loosened.
The water content of the soil in the nursery in the step 5) is 16%.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A breeding and planting method for tissue culture of a bitter rope is characterized by comprising the following steps:
1) the method comprises the following steps of (1) recovering the overground part of a tape, removing leaves, cutting off the upper half part with more growing points, carrying out oscillation washing for 18-22 min by using sterile water, carrying out oscillation washing for 2-3 min by using 0.05% detergent, rinsing the tape to be free of foam by using the sterile water, adding 70-75% of ethanol for 10-20 seconds for sterilization, carrying out three times of sterilization by using the sterile water, adding 0.05% of mercuric chloride solution for oscillation sterilization for 1-3 min, adding the sterile water with the same volume, continuing oscillation sterilization for 12-15 min, and carrying out five times of sterilization by using the sterile water to obtain a sterile material;
2) inoculating the sterile material into a culture medium 1/2MS + IAA 0.2mg/L, adding sucrose 15g/L, adjusting the pH to 5.8-6.0, illuminating at 2500lx at 22-24 ℃ for 12 h/d; obtaining a grown sterile material;
3) cutting off stems of the aseptic materials with buds growing in the step 2), inoculating the stems in a culture medium MS + BA 0.5mg/L + NAA 0.1mg/L + GA 0.5mg/L, and carrying out bud differentiation culture under the illumination condition to obtain differentiated cluster buds;
4) cutting the differentiated cluster buds in the step 3) into stem sections with terminal buds or 1-2 axillary buds, inoculating the stem sections on a culture medium 1/3MS + IAA 0.5mg/L for rooting culture, and obtaining rooting test-tube plantlets;
5) transplanting the rooting test-tube plantlets to a growth substrate, culturing for thirty days under the conditions that the temperature is 20-27 ℃, the humidity is 75-90% and no direct light exists, transplanting the obtained test-tube plantlets to a hillside-shaped nursery garden at the end of four months and in the last ten days of May, and building frames when the test-tube plantlets of the nursery garden grow to 28-32 cm.
2. The method for cultivating and breeding bitter rope tissue according to claim 1, wherein the method comprises the following steps: the growth substrate comprises furnace ash, humus, plant ash, sterilized chicken manure fermented for more than half a year, activated carbon and sucrose.
3. The method for cultivating and breeding bitter rope tissue according to claim 2, wherein the method comprises the following steps: the feeding ratio of the furnace ash, humus soil, plant ash, sterilized chicken manure fermented for more than half a year, active carbon and cane sugar is 1:1:1:0.5:0.02:0.3, and the raw materials are subjected to high-pressure high-temperature sterilization treatment and deinsectization treatment and then are uniformly stirred at normal temperature.
4. The method for cultivating and breeding bitter rope tissue according to claim 1, wherein the method comprises the following steps: and 5) spraying nutrient water every 7-10 days when the rooting test-tube plantlet grows for more than 3cm, and keeping ventilation for 2-5 hours.
5. The method for cultivating and breeding bitter rope tissue according to claim 1, wherein the method comprises the following steps: the growth substrate in the step 5) is kept in a state that the growth substrate can be grabbed by hands, can be kneaded into a cluster and can be dispersed after the hands are loosened.
6. The method for cultivating and breeding bitter rope tissue according to claim 1, wherein the method comprises the following steps: the water content of the soil in the nursery garden in the step 5) is 14-18%.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270947A (en) * | 2013-03-08 | 2013-09-04 | 浙江省农业科学院 | Duvalia angustiloba tissue culturing method |
| CN104719151A (en) * | 2015-02-27 | 2015-06-24 | 中国医学科学院药用植物研究所云南分所 | Rapid propagation method of dregea sinensis hemsl |
| PH22018000302U1 (en) * | 2018-04-12 | 2018-10-26 | Abra State Institute Of Sciences And Tech Main Campus | PROCESS OF PRODUCING SOIL MEDIA FOR STEM PROPAGATION OF AMPUPUYAT (Dregea volubilis) |
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- 2019-07-04 CN CN201910600827.9A patent/CN110140659B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270947A (en) * | 2013-03-08 | 2013-09-04 | 浙江省农业科学院 | Duvalia angustiloba tissue culturing method |
| CN104719151A (en) * | 2015-02-27 | 2015-06-24 | 中国医学科学院药用植物研究所云南分所 | Rapid propagation method of dregea sinensis hemsl |
| PH22018000302U1 (en) * | 2018-04-12 | 2018-10-26 | Abra State Institute Of Sciences And Tech Main Campus | PROCESS OF PRODUCING SOIL MEDIA FOR STEM PROPAGATION OF AMPUPUYAT (Dregea volubilis) |
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