CN110133772A - Aperture device and gene sequencer - Google Patents
Aperture device and gene sequencer Download PDFInfo
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- CN110133772A CN110133772A CN201910557509.9A CN201910557509A CN110133772A CN 110133772 A CN110133772 A CN 110133772A CN 201910557509 A CN201910557509 A CN 201910557509A CN 110133772 A CN110133772 A CN 110133772A
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 44
- 239000000758 substrate Substances 0.000 claims abstract description 39
- 239000012780 transparent material Substances 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/005—Diaphragms
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Abstract
The application provides a kind of aperture device and gene sequencer, is related to gene sequencing technology field.Wherein, the aperture device on substrate by being arranged the first fixation hole, the second fixation hole, first through hole and the second through-hole, while the reflected beams are blocked in realization, certain position adjustable range is provided, to improve aperture device to the adaptability of different light paths；The gene sequencer stops by using the aperture device to by the first light beam and the second light beam of capillary array, to prevent first light beam and the second light beam from reversely injecting laser after capillary array, laser damage or laser beam quality is caused to reduce.
Description
Technical Field
The application relates to the technical field of gene sequencing, in particular to a diaphragm device and a gene sequencer.
Background
In the light path of the gene sequencer, laser beams emitted by a laser form two beams of laser after passing through a beam splitter, and the two beams of laser are reflected and simultaneously irradiated to a sample to be detected from two opposite directions to excite fluorescence. Since the two laser beams have opposite directions, the two laser beams may be reversely emitted into the laser after passing through the sample to be detected, which may cause damage to the laser or deterioration of the quality of the laser beam.
On this basis, it is of great importance for the skilled person to develop a solution that overcomes the above-mentioned problems.
Disclosure of Invention
In view of the above, the present application provides an aperture device and a gene sequencer to solve the above problems in the prior art.
In order to achieve the above purpose, the preferred embodiment of the present application adopts the following technical solutions:
in a first aspect, an embodiment of the present application provides a diaphragm apparatus, which is applied to a gene sequencer;
the diaphragm device comprises a substrate made of non-transparent materials, wherein the substrate comprises a first through hole, a second through hole, a first fixing hole and a second fixing hole; wherein,
the first fixing hole and the second fixing hole are kidney-shaped holes;
the first fixing hole and the second fixing hole are arranged in parallel from one end of the substrate along a first direction of the substrate;
the first through hole and the second through hole are the same in shape and size, and are arranged in a staggered mode in a first direction of the substrate and a second direction perpendicular to the first direction.
Optionally, in this embodiment of the present application, the surface of the substrate is processed by matte processing.
Optionally, in this embodiment of the application, the shape of the first through hole and the second through hole includes any one of a circle, an ellipse, and a rectangle.
In a second aspect, an embodiment of the present application provides a gene sequencer, where the gene sequencer includes a laser, a capillary array, a first diaphragm device, and a second diaphragm device that is the same as the first diaphragm device; wherein,
the first diaphragm device and the second diaphragm device comprise substrates made of non-transparent materials, and the substrates comprise a first through hole, a second through hole, a first fixing hole and a second fixing hole;
the first fixing hole and the second fixing hole are kidney-shaped holes;
the first fixing hole and the second fixing hole are arranged in parallel from one end of the substrate along a first direction of the substrate;
the first through hole and the second through hole are the same in shape and size, and are arranged in a staggered mode in a first direction of the substrate and a second direction perpendicular to the first direction;
a first light beam generated by the laser irradiates the capillary array after passing through the first diaphragm device, and the first light beam is shielded by the second diaphragm device after passing through the capillary array;
and a second light beam generated by the laser irradiates the capillary array after passing through the second diaphragm device, and is shielded by the first diaphragm device after passing through the capillary array.
Optionally, in this embodiment, the shape and size of the first through hole and the second through hole are the same as the spot shape and size of the first light beam and the second light beam.
Optionally, in an embodiment of the present application, a surface of the substrate is formed by matte processing;
the first through hole and the second through hole have any one of a circular shape, an oval shape and a rectangular shape.
Optionally, in an embodiment of the present application, the gene sequencer comprises an optical splitter;
and splitting the laser emitted by the laser by the beam splitter to obtain the first light beam and the second light beam.
Optionally, in an embodiment of the present application, the gene sequencer further includes a first mirror, a second mirror, a first focusing lens, and a second focusing lens;
the first light beam passes through a first diaphragm device after being reflected by the first reflector, and is focused to the capillary array through the first focusing lens after passing through the first diaphragm device;
the second light beam passes through a second diaphragm device after being reflected by the second reflecting mirror, and is focused to the capillary array through the second focusing lens after passing through the second diaphragm device;
the first light beam is shielded by the second diaphragm device after passing through the capillary array, and the second light beam is shielded by the first diaphragm device after passing through the capillary array.
Optionally, in another embodiment of the present application, the gene sequencer further includes a first mirror, a second mirror, a first focusing lens, and a second focusing lens;
the first light beam passes through a first diaphragm device, is reflected to the first focusing lens through the first reflecting mirror, and is focused to the capillary array through the first focusing lens;
the second light beam passes through a second diaphragm device, is reflected to the second focusing lens by the second reflecting mirror and is focused to the capillary tube array by the second focusing lens;
the first light beam passes through the capillary array, is reflected to the second diaphragm device by the second reflector and is shielded by the second diaphragm device;
and the second light beam passes through the capillary array, is reflected to the first diaphragm device by the second reflector and is shielded by the first diaphragm device.
Optionally, in another embodiment of the present application, the gene sequencer includes a first laser, a second laser, a first mirror, a second mirror, a first focusing lens, and a second focusing lens; wherein,
the first laser is used for generating the first light beam, and the second laser is used for generating the second light beam;
the first light beam passes through a first diaphragm device, is reflected to the first focusing lens through the first reflecting mirror, and is focused to the capillary array through the first focusing lens;
the second light beam passes through a second diaphragm device, is reflected to the second focusing lens by the second reflecting mirror and is focused to the capillary tube array by the second focusing lens;
the first light beam is shielded by the second diaphragm device after passing through the capillary array, and the second light beam is shielded by the first diaphragm device after passing through the capillary array.
Compared with the prior art, the method has the following beneficial effects:
the diaphragm device that this application embodiment provided, through first fixed orifices and the second fixed orifices that sets up waist type and the first through-hole and the second through-hole of dislocation on the base plate, can provide certain position control scope when realizing sheltering from the reflected beam to improve the adaptability of diaphragm device to different light paths.
For the gene sequencer provided by the embodiment of the application, the diaphragm device is adopted to block the first light beam and the second light beam which pass through the capillary array, so that the first light beam and the second light beam can be prevented from reversely injecting into a laser after passing through a sample to be detected, and the laser is prevented from being damaged or the quality of the laser beam is reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.
Fig. 1 is a schematic structural diagram of an aperture device provided in an embodiment of the present application;
FIG. 2 is a schematic diagram of a first structure of a gene sequencer according to an embodiment of the present disclosure;
FIG. 3 is an enlarged partial schematic view of FIG. 2;
FIG. 4 is a schematic diagram of a second configuration of a gene sequencer provided in an embodiment of the present application;
FIG. 5 is a schematic diagram of a third structure of the gene sequencer provided in the embodiments of the present application.
Icon: 10-a substrate; 11-a first via; 12-a second via; 13-a first fixation hole; 14-a second fixation hole; 21-a first diaphragm arrangement; 22-a second diaphragm arrangement; 23-a capillary array; 24-a first light beam; 25-a second light beam; 26-a first mirror; 27-a second mirror; 28-a first focusing lens; 29-second focusing lens.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present application, it should be noted that the terms "first", "second", etc. are named only for distinguishing different features of the present application, and the description is simplified, but does not indicate or imply relative importance, and thus, should not be construed as limiting the present application.
Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.
In a gene sequencer, a laser is usually used as an excitation light source to detect a capillary array, different fluorescence emitted by the laser represents different base information, and the different fluorescence emitted by the laser can be converted into a DNA sequence by synchronous imaging on a CCD (Charge coupled device) camera, so that the purpose of DNA sequencing is achieved.
Because the capillary has certain width, if the capillary is irradiated by laser from only one direction, the fluorescent intensity generated by the capillary is inconsistent because the illumination intensity of one side is stronger and the illumination intensity of the other side is weaker, and the signal processing in the later period is influenced. Therefore, in order to ensure the accuracy of the detection result in the prior art, two laser beams are generally used to irradiate the capillary array from opposite directions simultaneously.
However, in practical applications, the two laser beams have small deviation after passing through the capillary array, and a situation that a laser beam is reversely incident to a laser along the optical path of the other laser beam, so that the quality of the laser beam is reduced, and even the laser is damaged occurs.
Based on the above problem, the present application provides an aperture device, which can be applied to a gene sequencer to realize blocking another light beam from reversely emitting back to a laser beam while emitting the light beam.
Specifically, in the embodiment of the present application, the aperture device includes a substrate made of a non-transparent material, such as aluminum, iron, or the like, so that the laser beam is shielded by the substrate after being irradiated onto the substrate.
Referring to fig. 1, the substrate 10 is provided with a first through hole 11 and a second through hole 12 through which a laser beam passes, and a first fixing hole 13 and a second fixing hole 14 for fixing the substrate 10.
In a possible embodiment, the first fixing hole 13 and the second fixing hole 14 are each designed as a kidney-shaped hole, and the first fixing hole 13 and the second fixing hole 14 are arranged in parallel from one end of the base plate 10 along a first direction of the base plate 10, so that the base plate 10 can be adjusted to a certain extent along a length extension direction of the first fixing hole 13 and the second fixing hole 14 during fixing.
With reference to fig. 1, the first through hole 11 and the second through hole 12 may have the same shape and size, and the first through hole 11 and the second through hole 12 are disposed in a staggered manner in a first direction of the substrate 10 and a second direction perpendicular to the first direction.
The first through hole 11 and the second through hole 12 are designed to be the same in shape and size, and can be switched to adapt to laser beams with different heights in the application process; the first through hole 11 and the second through hole 12 are arranged in a staggered manner, so that a light beam can pass through while another light beam is blocked.
Specifically, the first fixing hole 13 and the second fixing hole 14, and the first through hole 11 and the second through hole 12 which are arranged in a staggered manner can be used for adjusting in the first direction and the second direction, so that the applicability of the diaphragm device to different application scenes is improved.
In other words, in the present embodiment, the relative position of the diaphragm device and the laser beam can be adjusted by the first fixing hole 13 and the second fixing hole 14, and meanwhile, the laser beams with different heights can be accommodated by the first through hole 11 and the second through hole 12 which are located at different heights.
Further, in another possible embodiment, the surface of the substrate 10 may be matte-treated, so as to avoid the blocked light beam from reflecting on the substrate 10.
In the embodiment of the present application, the shapes of the first through hole 11 and the second through hole 12 may be set according to the shape and the size of the light spot. For example, in one possible design, the first through-hole 11 and the second through-hole 12 may be designed to be circular, and in another possible design, the first through-hole 11 and the second through-hole 12 may also be designed to be oval or rectangular.
It should be further noted that, in the embodiment of the present application, the diaphragm device may include one or more of the above-mentioned base plates 10, and the base plates 10 may include, but are not limited to, the above-mentioned first through hole 11, second through hole 12, first fixing hole 13, and second fixing hole 14. Also, the shape of the first fixing hole 13 and the second fixing hole 14 may be, but is not limited to, a kidney-shaped hole; the first through hole 11 and the second through hole 12 may have other shapes than the above-described circular, elliptical, and rectangular shapes.
Referring to fig. 2 and fig. 3, a schematic structural diagram of a gene sequencer is provided for an embodiment of the present application, wherein fig. 3 is a partially enlarged schematic diagram of fig. 2. The gene sequencer comprises a laser, a capillary array 23, a first diaphragm device 21 and a second diaphragm device 22 which is the same as the first diaphragm device 21.
In the embodiment of the present application, the structures of the first aperture device 21 and the second aperture device 22 may be the same as those of the aperture devices provided in any of the above-described embodiments.
The first light beam 24 generated by the laser is irradiated to the capillary array 23 after passing through the first aperture device 21, and the first light beam 24 is blocked by the second aperture device 22 after passing through the capillary array 23 (as shown in fig. 3). Meanwhile, a second light beam 25 generated by the laser is irradiated to the capillary array 23 after passing through the second diaphragm device 22, and the second light beam 25 is blocked by the first diaphragm device 21 after passing through the capillary array 23.
Specifically, by adding the first aperture device 21 and the second aperture device 22 to the gene sequencer, the first light beam 24 and the second light beam 25 can be blocked after passing through the capillary array 23, so that the laser can be prevented from being damaged due to backward incidence of the first light beam and the second light beam into the laser.
In the embodiment of the present application, the shape and size of the first through hole 11 and the second through hole 12 may be the same as the spot shape and size of the first light beam 24 and the second light beam 25, so as to ensure that the first through hole 11 and the second through hole 12 just can pass through one of the light beams and block the other light beam. For example, when the spot shapes of the first and second light beams 24 and 25 are circular, the shapes of the first and second through holes 11 and 12 may be designed to be circular; when the spot shapes of the first and second light beams 24 and 25 are rectangular, the shapes of the first and second through holes 11 and 12 may also be rectangular.
It should be noted that in the embodiment of the present application, the first light beam 24 and the second light beam 25 may be generated by the same laser or different lasers.
For example, in one possible embodiment, the gene sequencer includes a first laser for generating the first beam 24 and a second laser for generating the second beam 25.
Alternatively, in another possible embodiment, the gene sequencer may comprise only one laser, and the laser emitted by the laser may be split by a beam splitter to obtain the first beam 24 and the second beam 25.
In the present embodiments, the gene sequencer may include, but is not limited to, the components described above. In one possible embodiment, as shown in fig. 4, the gene sequencer may further include a first mirror 26, a second mirror 27, a first focusing lens 28 and a second focusing lens 29.
The first mirror 26 and the second mirror 27 are used for reflecting the first light beam 24 and the second light beam 25, respectively, so as to change the incidence directions of the first light beam 24 and the second light beam 25 to simultaneously irradiate the capillary array 23 from approximately opposite directions.
In the embodiment of the present application, the first mirror 26 and the second mirror 27 can be angularly adjusted within a certain range, and when the paths of the first beam 24 and the second beam 25 are completely overlapped, so that the beam reflected back to the laser cannot be blocked, the angles of the first mirror 26 and the second mirror 27 can be adjusted to relatively deviate the first beam 24 and the second beam 25, so that one beam can pass through the first through hole 11 or the second through hole 12, and the other beam is blocked by the substrate 10.
Moreover, in the embodiment of the present application, it is also possible to select an appropriate through hole to pass through the first light beam 24 and the second light beam 25 by adjusting the relative positions of the substrate 10 and the first light beam 24 and the second light beam 25. In other words, in the embodiment of the present application, the first light beam 24 may pass through the substrate 10 through the first through hole 11, or may pass through the substrate 10 through the second through hole 12, and the second light beam 25 is the same, and will not be described herein again.
With continued reference to fig. 4, in this embodiment, the first light beam 24 passes through the first aperture device 21 after being reflected by the first mirror 26, and is focused to the capillary array 23 through the first focusing lens 28 after passing through the first aperture device 21; the second light beam 25 passes through the second diaphragm device 22 after being reflected by the second reflecting mirror 27, and is focused to the capillary array 23 through the second focusing lens 29 after passing through the second diaphragm device 22; the first light beam 24 is blocked by the second diaphragm device 22 after passing through the capillary array 23, and the second light beam 25 is blocked by the first diaphragm device 21 after passing through the capillary array 23.
Alternatively, referring to fig. 5, in another possible embodiment, the first light beam 24 passes through the first aperture device 21 after being redirected by the first mirror 26, and is focused on the capillary array 23 by the first focusing lens 28 after passing through the first aperture device 21; the second light beam 25 passes through the second diaphragm device 22, is reflected to the second focusing lens 29 by the second reflecting mirror 27, and is focused to the capillary array 23 by the second focusing lens 29; the first light beam 24 passes through the capillary array 23, is reflected to the second diaphragm device 22 by the second reflecting mirror 27, and is blocked by the second diaphragm device 22; the second light beam 25 passes through the capillary array 23, is reflected by the second reflector 27 to the first aperture device 21, and is blocked by the first aperture device 21.
It should be understood that the devices disclosed in the embodiments of the present application may be implemented in other ways. The above-described embodiments of the apparatus are merely illustrative, and for example, in one possible embodiment, the gene sequencer may include other mirrors besides the first mirror 26 and the second mirror 27 to change the propagation direction of the light beam multiple times, so as to adapt the structure of the gene sequencer and fully utilize the useful space of the gene sequencer. In the embodiment of the present invention, the first aperture device 21 and the second aperture device 22 may be installed at other positions than the above-described embodiments.
In summary, the embodiments of the present application provide a flat diaphragm device and a gene sequencer. The diaphragm device is provided with the waist-shaped first fixing hole and the waist-shaped second fixing hole as well as the staggered first through hole and the staggered second through hole on the substrate, so that a reflected light beam is shielded, a certain position adjusting range is provided, and the adaptability of the diaphragm device to different light paths is improved. The gene sequencer blocks the first light beam and the second light beam which pass through the capillary array by adopting the diaphragm device, so that the first light beam and the second light beam are prevented from reversely injecting into the laser after passing through a sample to be detected, and the laser is prevented from being damaged or the quality of the laser beam is reduced.
The above-mentioned embodiments are only specific embodiments of the present application, and are used for illustrating the technical solutions of the present application, but not limiting the same, and the scope of the present application is not limited thereto, and although the present application is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: any person skilled in the art can modify or easily conceive the technical solutions described in the foregoing embodiments or equivalent substitutes for some technical features within the technical scope disclosed in the present application; such modifications, changes or substitutions do not depart from the spirit and scope of the exemplary embodiments of the present application, and are intended to be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (10)
1. A diaphragm device is characterized by being applied to a gene sequencer;
the diaphragm device comprises a substrate made of non-transparent materials, wherein the substrate comprises a first through hole, a second through hole, a first fixing hole and a second fixing hole; wherein,
the first fixing hole and the second fixing hole are kidney-shaped holes;
the first fixing hole and the second fixing hole are arranged in parallel from one end of the substrate along a first direction of the substrate;
the first through hole and the second through hole are the same in shape and size, and are arranged in a staggered mode in a first direction of the substrate and a second direction perpendicular to the first direction.
2. An optical diaphragm apparatus according to claim 1, wherein the surface of the substrate is matte-treated.
3. The diaphragm apparatus according to claim 1 or 2, wherein the shape of said first through hole and said second through hole includes any one of a circle, an ellipse, and a rectangle.
4. A gene sequencer is characterized by comprising a laser, a capillary array, a first diaphragm device and a second diaphragm device which is the same as the first diaphragm device; wherein,
the first diaphragm device and the second diaphragm device comprise substrates made of non-transparent materials, and the substrates comprise a first through hole, a second through hole, a first fixing hole and a second fixing hole;
the first fixing hole and the second fixing hole are kidney-shaped holes;
the first fixing hole and the second fixing hole are arranged in parallel from one end of the substrate along a first direction of the substrate;
the first through hole and the second through hole are the same in shape and size, and are arranged in a staggered mode in a first direction of the substrate and a second direction perpendicular to the first direction;
a first light beam generated by the laser irradiates the capillary array after passing through the first diaphragm device, and the first light beam is shielded by the second diaphragm device after passing through the capillary array;
and a second light beam generated by the laser irradiates the capillary array after passing through the second diaphragm device, and is shielded by the first diaphragm device after passing through the capillary array.
5. The gene sequencer of claim 4, wherein the first via and the second via have a shape and a size that is the same as a spot shape and a size of the first beam and the second beam.
6. The gene sequencer of claim 4 or 5, wherein the surface of the substrate is matte-treated;
the first through hole and the second through hole have any one of a circular shape, an oval shape and a rectangular shape.
7. The gene sequencer of claim 6, wherein the gene sequencer comprises an optical splitter;
and splitting the laser emitted by the laser by the beam splitter to obtain the first light beam and the second light beam.
8. The gene sequencer of claim 7, further comprising a first mirror, a second mirror, a first focusing lens, and a second focusing lens;
the first light beam passes through a first diaphragm device after being reflected by the first reflector, and is focused to the capillary array through the first focusing lens after passing through the first diaphragm device;
the second light beam passes through a second diaphragm device after being reflected by the second reflecting mirror, and is focused to the capillary array through the second focusing lens after passing through the second diaphragm device;
the first light beam is shielded by the second diaphragm device after passing through the capillary array, and the second light beam is shielded by the first diaphragm device after passing through the capillary array.
9. The gene sequencer of claim 7, further comprising a first mirror, a second mirror, a first focusing lens, and a second focusing lens;
the first light beam passes through a first diaphragm device, is reflected to the first focusing lens through the first reflecting mirror, and is focused to the capillary array through the first focusing lens;
the second light beam passes through a second diaphragm device, is reflected to the second focusing lens by the second reflecting mirror and is focused to the capillary tube array by the second focusing lens;
the first light beam passes through the capillary array, is reflected to the second diaphragm device by the second reflector and is shielded by the second diaphragm device;
and the second light beam passes through the capillary array, is reflected to the first diaphragm device by the second reflector and is shielded by the first diaphragm device.
10. The gene sequencer according to claim 4 or 5, wherein the gene sequencer comprises a first laser, a second laser, a first mirror, a second mirror, a first focusing lens, and a second focusing lens; wherein,
the first laser is used for generating the first light beam, and the second laser is used for generating the second light beam;
the first light beam passes through a first diaphragm device, is reflected to the first focusing lens through the first reflecting mirror, and is focused to the capillary array through the first focusing lens;
the second light beam passes through a second diaphragm device, is reflected to the second focusing lens by the second reflecting mirror and is focused to the capillary tube array by the second focusing lens;
the first light beam is shielded by the second diaphragm device after passing through the capillary array, and the second light beam is shielded by the first diaphragm device after passing through the capillary array.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910557509.9A CN110133772B (en) | 2019-06-25 | 2019-06-25 | Diaphragm device and gene sequencer |
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| CN201910557509.9A CN110133772B (en) | 2019-06-25 | 2019-06-25 | Diaphragm device and gene sequencer |
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| CN110133772B CN110133772B (en) | 2024-06-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022095025A1 (en) * | 2020-11-09 | 2022-05-12 | 南京溯远基因科技有限公司 | Diaphragm adjustment structure, diaphragm device and gene sequencing apparatus |
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