CN110118774B - Water plankton detection device and method - Google Patents
Water plankton detection device and method Download PDFInfo
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- CN110118774B CN110118774B CN201910553491.5A CN201910553491A CN110118774B CN 110118774 B CN110118774 B CN 110118774B CN 201910553491 A CN201910553491 A CN 201910553491A CN 110118774 B CN110118774 B CN 110118774B
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 328
- 238000001514 detection method Methods 0.000 title claims abstract description 150
- 238000000034 method Methods 0.000 title claims description 28
- 238000007667 floating Methods 0.000 claims abstract description 34
- 238000005070 sampling Methods 0.000 claims abstract description 19
- 230000002572 peristaltic effect Effects 0.000 claims description 43
- 230000008878 coupling Effects 0.000 claims description 39
- 238000010168 coupling process Methods 0.000 claims description 39
- 238000005859 coupling reaction Methods 0.000 claims description 39
- 239000013307 optical fiber Substances 0.000 claims description 39
- 230000001105 regulatory effect Effects 0.000 claims description 34
- 230000001276 controlling effect Effects 0.000 claims description 31
- 238000007599 discharging Methods 0.000 claims description 7
- 238000003384 imaging method Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 239000002352 surface water Substances 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0118—Apparatus with remote processing
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hydrology & Water Resources (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a water surface plankton detection device. The detection device comprises a sampling system and a detection system; the sampling system comprises a water taking head, a self-priming water pump and a transfer device; the water taking head comprises a floating pipe and a water taking pipe; the floating pipe and the water intake pipe are annular hollow hoses, and the water intake pipe is fixedly adhered to the outer side of the floating pipe; the outer side of the water intake pipe is provided with a plurality of water inlets and an interface; the interface is connected with a water inlet pipe of the self-priming water pump; the transfer device is connected with a water outlet pipe of the self-priming water pump; the transfer device is connected with the detection system. Through setting up the water intaking head, under the control of inhaling the water pump through the inlet opening direct suction surface of water's water sample, in carrying the shifter, carry detecting to detecting system by the shifter again, ensure that the water sample is not destroyed, reduce the damage to plankton in the water sample.
Description
Technical Field
The invention relates to the technical field of plankton detection, in particular to a device and a method for detecting water plankton.
Background
At present, an underwater camera used in the detection of the plankton on the water surface is only suitable for imaging large-scale aquatic organisms, and for plankton with the body size of micrometer to millimeter magnitude, microscopic amplification is generally required for imaging detection, but the microscope has small depth and is not suitable for imaging detection of plankton swimming in a 3-dimensional space of a water body.
In abroad, detection of plankton in water body by digital holographic technology has been reported. Because the digital hologram has digital focusing characteristic, a hologram is shot and recorded, and the reconstruction image of an object in a plane with different distances from the image sensor can be obtained by setting different reconstruction distances during reconstruction, the method is particularly suitable for imaging detection of plankton swimming in a 3-dimensional space of a water body.
However, under natural conditions, there is a large fluctuation in the water surface, and the fluctuation in the water surface causes a strong irregular refraction phenomenon when the light beam passes through the water surface, so that digital holographic imaging detection cannot be directly performed. Therefore, the existing method adopted in the detection of the plankton on the water surface firstly utilizes a sampling net to collect the plankton, and then the plankton is brought back to a laboratory for detection, and the method inevitably causes certain damage to the plankton, thereby influencing the authenticity of the detection result.
Disclosure of Invention
The invention aims to provide a device and a method for detecting plankton on the water surface, which can reduce the damage to plankton.
In order to achieve the above object, the present invention provides the following solutions:
a water plankton detection device, comprising a sampling system and a detection system;
the sampling system comprises a water taking head, a self-priming water pump and a transfer device;
the water taking head comprises a floating pipe and a water taking pipe;
the floating pipe and the water intake pipe are annular hollow hoses, and the water intake pipe is fixedly stuck to the outer side of the floating pipe; the floating pipe is used for floating the water taking head on the water surface under the action of buoyancy;
the outer side of the water intake pipe is provided with a plurality of water inlets and an interface;
the interface is connected with a water inlet pipe of the self-priming water pump;
the transfer device is connected with a water outlet pipe of the self-priming water pump; the self-priming water pump is used for controlling the water taking head to absorb a water sample on the water surface through the water inlet hole and conveying the water sample into the transfer device;
the transfer device is connected with the detection system and is used for separating bubbles in the water sample and conveying the separated water sample to the detection device for detection.
Optionally, the shifter is provided with a water inlet, an air outlet and a water outlet;
the water inlet and the air outlet are both arranged at the top of the shifter;
the water outlet is arranged at the bottom of the shifter;
the water inlet is connected with a water outlet pipe of the self-priming water pump;
the exhaust port is connected with the first return pipe and is used for exhausting bubbles in the water sample and exhausting the water sample in the transfer device;
the water outlet is connected with the detection system through a hose.
Optionally, the detection system comprises a detection cell and a peristaltic pump;
the detection pool is of a closed square structure;
the side surface of the detection tank is provided with a sample inlet and a sample outlet;
the sample inlet is connected with the water outlet;
the sample outlet is connected with the input end of the peristaltic pump;
the output end of the peristaltic pump is connected with a second return pipe;
the peristaltic pump is used for controlling the water sample in the transfer device to be transferred into the detection tank for detection, and the water sample in the detection tank is circulated into the water through the second return pipe.
Optionally, the detection system further comprises a digital camera and an optical fiber coupling output laser;
the digital camera and the optical fiber coupling output laser are arranged oppositely, so that laser beams emitted by the optical fiber coupling output laser are normally incident on an image sensor of the digital camera through a detection pool;
the digital camera is used for shooting and recording digital holograms.
Optionally, the detection device further comprises a power supply system and a switch circuit;
the power supply system comprises a battery, a first voltage regulating module and a second voltage regulating module;
the switching circuit comprises a first switching circuit, a second switching circuit and a third switching circuit;
the battery is respectively connected with one end of the first switch circuit, one end of the second switch circuit and one end of the third switch circuit;
the other end of the first switch circuit is connected with the optical fiber coupling output laser through the first voltage regulating module and connected with the digital camera through the second voltage regulating module; the first voltage regulating module is used for converting the battery voltage into the voltage required by the optical fiber coupling output laser; the second voltage regulating module is used for converting the battery voltage into the voltage required by the digital camera;
the other end of the second switch circuit is connected with the peristaltic pump;
the other end of the third switch circuit is connected with the self-priming pump.
Optionally, the switch of the first switch circuit is a click switch;
the switches of the second switch circuit and the third switch circuit are delay switches.
Optionally, the detection device further comprises a remote control signal receiving module and a remote controller;
the remote control signal receiving module is respectively connected with the control end of the first switch circuit, the control end of the second switch circuit, the control end of the third switch circuit and the shutter switch of the digital camera;
the remote controller is used for sending a remote control signal to the remote control signal receiving module;
the remote control signal receiving module is used for controlling corresponding circuit actions according to the remote control signals.
A method for detecting plankton on water surface, which is applied to a device for detecting plankton on water surface, the device comprises: a sampling system and a detection system;
the sampling system comprises a water taking head, a self-priming water pump and a transfer device;
the water taking head comprises a floating pipe and a water taking pipe;
the floating pipe and the water intake pipe are annular hollow hoses, and the water intake pipe is fixedly stuck to the outer side of the floating pipe; the outer side of the water intake pipe is provided with a plurality of water inlets and an interface;
the interface is connected with a water inlet pipe of the self-priming water pump; the transfer device is connected with a water outlet pipe of the self-priming water pump; the transfer device is connected with the detection system;
the detection system comprises a detection pool, a peristaltic pump, a digital camera and an optical fiber coupling output laser;
the side surface of the detection tank is provided with a sample inlet and a sample outlet; the sample inlet is connected with the water outlet; the sample outlet is connected with the input end of the peristaltic pump; the output end of the peristaltic pump is connected with a second return pipe;
the digital camera and the optical fiber coupling output laser are arranged oppositely, so that laser beams emitted by the optical fiber coupling output laser are normally incident on an image sensor of the digital camera through a detection pool;
the detection device further comprises a power supply system, a switch circuit, a remote control signal receiving module and a remote controller;
the power supply system comprises a battery, a first voltage regulating module and a second voltage regulating module;
the switching circuit comprises a first switching circuit, a second switching circuit and a third switching circuit;
the battery is respectively connected with one end of the first switch circuit, one end of the second switch circuit and one end of the third switch circuit;
the other end of the first switch circuit is connected with the optical fiber coupling output laser through the first voltage regulating module and connected with the digital camera through the second voltage regulating module;
the other end of the second switch circuit is connected with the peristaltic pump;
the other end of the third switch circuit is connected with the self-priming pump;
the switch of the first switch circuit is a inching switch;
the switches of the second switch circuit and the third switch circuit are delay switches;
the remote control signal receiving module is respectively connected with the control end of the first switch circuit, the control end of the second switch circuit, the control end of the third switch circuit and the shutter switch of the digital camera;
the method comprises the following steps:
the third switch circuit is controlled to be turned on, the water sampling head is controlled to absorb the water sample on the water surface through the self-priming pump, and the water sample is conveyed into the transfer device;
controlling the first switch circuit to be turned on to start the digital camera and the optical fiber coupling output laser;
controlling a second switch circuit to be switched on, controlling the water sample in the shifter to be shifted into a detection pool through a peristaltic pump, and circularly discharging the water sample in the detection pool;
controlling the digital camera to shoot and record a digital hologram;
repeating the steps of controlling the second switch circuit to be turned on, transferring the water sample in the transfer device to a detection pool through peristaltic pump control, circularly discharging the water sample in the detection pool until the step of controlling the digital camera to shoot and record a digital hologram until the water sample in the transfer device is completely detected;
and controlling the first switch circuit to be closed, so that the digital camera and the optical fiber coupling output laser are powered off.
Optionally, the third switch circuit is controlled to be turned on, the water sample on the water surface is sucked by the self-priming pump and the water sample is conveyed into the transfer device, and the method specifically comprises the following steps:
setting the delay time of automatic closing after the third switch circuit is turned on as the ratio of the volume of the transfer device to the flow rate of the self-priming water pump;
and sending an on control instruction to the third switch circuit.
Optionally, the second switch circuit is controlled to be turned on, the peristaltic pump is used for controlling the water sample in the shifter to be shifted into the detection tank, and the water sample in the detection tank is circularly discharged, and the method specifically comprises the following steps:
setting the delay time of automatic closing after the second switch circuit is switched on as the ratio of the volume of the detection pool to the flow rate of the peristaltic pump;
and sending an on control instruction to the second switch circuit.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects:
the water taking head is arranged, and the water taking head comprises a floating pipe and a water taking pipe; the floating pipe and the water intake pipe are annular hollow hoses, and the water intake pipe is fixedly stuck to the outer side of the floating pipe; a plurality of water inlets and an interface are arranged on the outer side of the water intake pipe; the interface is connected with a water inlet pipe of the self-priming water pump; can directly absorb the water sample of surface of water through the inlet opening under the control of inhaling the water pump, carry in the transfer ware, carry detecting system to detect by the transfer ware again, ensure that the water sample is not destroyed, reduce the damage to plankton in the water sample.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block diagram of a water surface plankton detection device according to an embodiment of the present invention;
FIG. 2 is a diagram of a water intake head according to an embodiment of the present invention;
FIG. 3 is a flowchart of a method for detecting plankton on water surface according to an embodiment of the present invention;
reference numerals illustrate:
the device comprises a battery, a first switch circuit, a first voltage regulating module, a second voltage regulating module, a 5-optical fiber coupling output laser, a second switch circuit, a remote control signal receiving module, an 8-exhaust port, a third switch circuit, a 10-water intake head, a self-priming water pump, a 12-water intake pipe, a 13-water outlet, a 14-detection pool, a 15-digital camera, a 16-peristaltic pump, a 17-second backflow pipe, a 18-shifter, a 19-water intake port, a 20-first backflow pipe, a 21-water inlet hole, a 22-floatation pipe, a 23-water intake pipe and a 24-interface.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a device and a method for detecting plankton on the water surface, which can reduce the damage to plankton.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
FIG. 1 is a block diagram of a water surface plankton detection device according to an embodiment of the present invention, as shown in FIG. 1, a water surface plankton detection device, where the detection device includes a sampling system and a detection system;
the sampling system comprises a water intake head 10, a self-priming water pump 11 and a shifter 18;
fig. 2 is a diagram of a water intake head according to an embodiment of the present invention, as shown in fig. 2, the water intake head 10 includes a floating pipe 22 and a water intake pipe 23;
the floating pipe 22 and the water intake pipe 23 are annular hollow hoses, and the water intake pipe 23 is fixedly adhered to the outer side of the floating pipe 22; the floating pipe 22 is used for floating the water taking head 10 on the water surface under the action of buoyancy;
the outside of the water intake pipe 23 is provided with a plurality of water inlets 21 and an interface 24;
the interface 24 is connected with the water inlet pipe 12 of the self-priming water pump 11.
In this embodiment, the floating pipe 22 and the water intake pipe 23 are hoses with diameters of 1cm, and the diameter of the water inlet 21 is set to 2-3 mm. Because the floating pipe 22 and the water intake pipe 23 are softer, when the water intake head 10 is placed on the water surface, the water intake pipe can undulate along with the water surface, so that the water intake hole 21 on the outer side of the water intake pipe 23 is just positioned on the water surface, and only the water sample on the water surface is ensured to be pumped during pumping.
The shifter 18 is connected with a water outlet pipe of the self-priming water pump 11; the self-priming water pump 11 is used for controlling the water taking head 10 to absorb water sample on the water surface through the water inlet hole 21 and conveying the water sample into the transfer device 18;
the transfer device 18 is connected with the detection system and is used for separating bubbles in the water sample and conveying the separated water sample to the detection device for detection.
The diverter 18 is provided with a water inlet 19, an air outlet 8 and a water outlet 13;
the water inlet 19 and the air outlet 8 are arranged at the top of the shifter 18;
the water outlet 13 is arranged at the bottom of the shifter 18;
the water inlet 19 is connected with a water outlet pipe of the self-priming water pump 11;
the exhaust port 8 is connected with a first return pipe 20 and is used for exhausting bubbles in the water sample and exhausting the water sample in the transfer device 18;
the water outlet 13 is connected with the detection system through a hose.
In this embodiment, the transfer device 18 is a sealed container with a volume of 200-500 mL. The air bubbles generated in the water suction process of the water suction head 10 are discharged through the transfer device 18, and then the water sample is detected, so that the detection authenticity and detection accuracy can be effectively improved.
The detection system includes a detection cell 14 and a peristaltic pump 16;
the detection cell 14 is of a closed square structure;
the side surface of the detection tank 14 is provided with a sample inlet and a sample outlet;
the sample inlet is connected with the water outlet 13;
the sample outlet is connected with the input end of the peristaltic pump 16;
the output end of the peristaltic pump 16 is connected with a second return pipe 17;
the peristaltic pump 16 is used for controlling the water sample in the transfer device 18 to be transferred into the detection tank 14 for detection, and circulating the water sample in the detection tank 14 into the water through the second return pipe 17.
The detection system also comprises a digital camera 15 and an optical fiber coupling output laser 5;
the digital camera 15 and the optical fiber coupling output laser 5 are arranged oppositely, so that the laser beam emitted by the optical fiber coupling output laser 5 is normally incident on the image sensor of the digital camera 15 through the detection pool 14;
the digital camera 15 is used for shooting and recording digital holograms.
In this embodiment, the relative positions of the optical fiber coupling output laser 5 and the digital camera 15 shown in fig. 1 are set, that is, the optical fiber coupling output laser 5 is disposed right above the detection cell 14, so as to emit laser light to irradiate the water sample in the detection cell 14; the digital camera 15 is arranged directly below the detection cell 14 for recording digital holograms.
In this embodiment, the digital camera 15 is a digital camera without an imaging lens, and the divergent laser beam emitted by the optical fiber coupling output laser 5 must be irradiated onto the image sensor of the digital camera through the detection cell 14, and the upper and lower surfaces of the detection cell 14 are made of flat optical transparent materials, so that the laser beam is not affected, and the detection accuracy is ensured. When in installation, the detection pool 14 is close to the digital camera 15, so that the digital hologram shot by the digital camera 15 is more accurate.
In this embodiment, the size of the transparent parts on the upper and lower bottom surfaces of the detection cell 14 is also set to be the same as the size of the image sensor of the digital camera 15. The images recorded on the digital camera 15 are called digital holograms and the images of plankton at different positions in the detection cell 14 can be obtained by using the on-axis digital hologram reconstruction algorithm.
In this embodiment, the thickness of the detection cell 14 is set to d, the fiber coupling output laser 5 is set to emit a laser beam through one fiber, and the distance L from the fiber to the digital camera 15 is far greater than the thickness d of the detection cell 14, so as to obtain a volume of the detected water sample corresponding to a single digital hologram as s×d. For example, taking an APS-framed image sensor (1.5 cm wide and 2.5cm long) as the detection cell 14 thickness d=5 cm, the volume of the water sample detected by a single hologram is 1.5cm×2.5cm×5cm.
The detection device further comprises a power supply system and a switch circuit;
the power supply system comprises a battery 1, a first voltage regulating module 3 and a second voltage regulating module 4;
the switching circuit comprises a first switching circuit 2, a second switching circuit 6 and a third switching circuit 9;
the battery 1 is respectively connected with one end of the first switch circuit 2, one end of the second switch circuit 6 and one end of the third switch circuit 9;
the other end of the first switch circuit 2 is connected with the optical fiber coupling output laser 5 through the first voltage regulating module 3 and is connected with the digital camera 15 through the second voltage regulating module 4; the first voltage regulating module 3 is used for converting the voltage of the battery 1 into the voltage required by the optical fiber coupling-out laser 5; the second voltage regulating module 4 is used for converting the voltage of the battery 1 into the voltage required by the digital camera 15;
the other end of the second switch circuit 6 is connected with the peristaltic pump 16;
the other end of the third switch circuit 9 is connected with the self-priming pump 11.
In this embodiment, the power supply voltage of the battery 1 is set to be 12V for the self-priming pump 11 and the peristaltic pump 16, the voltage required by the optical fiber coupling output laser 5 is usually 3V for the semiconductor laser, and the voltage of the battery 1 is converted into the voltage required by the optical fiber coupling output laser 5 through the first voltage regulating module 3; meanwhile, the voltage required by the digital camera 15 in the present invention is 7.8V, and the voltage of the battery 1 is converted into the voltage required by the digital camera 15 through the second voltage regulating module 4.
The switch of the first switch circuit 2 is a inching switch;
the switches of the second switching circuit 6 and the third switching circuit 9 are delay switches.
In this embodiment, the delay time of automatic closing after the third switch circuit 9 is turned on is set to be the ratio of the volume of the transfer device 18 to the flow rate of the self-priming pump 11; the delay time of the automatic closing after the second switch circuit 6 is turned on is set to be the ratio of the volume of the detection cell 14 to the flow rate of the peristaltic pump 16.
The detection device also comprises a remote control signal receiving module 7 and a remote controller; the remote control signal receiving module 7 and the remote controller adopted in the embodiment can be realized by adopting a common device capable of realizing remote control, and the remote controller sends control information to the remote control signal receiving module 7 in a wireless control mode.
The remote control signal receiving module 7 is respectively connected with the control end of the first switch circuit 2, the control end of the second switch circuit 6, the control end of the third switch circuit 9 and the shutter switch of the digital camera 15;
the remote controller is used for sending a remote control signal to the remote control signal receiving module 7;
the remote control signal receiving module 7 is used for controlling corresponding circuit actions according to the remote control signals.
The water surface plankton detection device in the implementation uses a remote control unmanned ship as a carrier and is used for detecting different positions of a water area.
When the biological device is applied, the self-priming water pump 11 pumps the surface water of the detection water area into the shifter 18, and the generated bubbles float upwards in the shifter 18 and are discharged through the air outlet 8 at the top of the shifter 18, and meanwhile, the bubbles cannot reach the position of the water outlet 13, so that the bubbles cannot enter the detection tank 14, and the purpose of removing the bubbles is achieved. Excess water after the diverter 18 is filled can also be drained through the vent 8 via the first return line 20. Peristaltic pump 16 is operated to draw the water sample from the diverter 18 into the test cell 14. After the digital hologram is obtained by shooting by the digital camera 15, the digital hologram is input into a computer for digital hologram reconstruction, so that images of plankton at different positions in the detection pool 14 can be obtained, and the detection of plankton on the water surface of the detection water area is realized.
Fig. 3 is a flowchart of a method for detecting water surface plankton according to an embodiment of the present invention, and as shown in fig. 3, the present invention further provides a method for detecting water surface plankton, which is applied to a device for detecting water surface plankton, where the device includes: a sampling system and a detection system;
the sampling system comprises a water intake head 10, a self-priming water pump 11 and a shifter 18;
the water intake head 10 comprises a floating pipe 22 and a water intake pipe 23;
the floating pipe 22 and the water intake pipe 23 are annular hollow hoses, and the water intake pipe 23 is fixedly adhered to the outer side of the floating pipe 22; the outside of the water intake pipe 23 is provided with a plurality of water inlets 21 and an interface 24;
the interface 24 is connected with the water inlet pipe 12 of the self-priming water pump 11; the shifter 18 is connected with a water outlet pipe of the self-priming water pump 11; the translator 18 is connected to the detection system;
the detection system comprises a detection pool 14, a peristaltic pump 16, a digital camera 15 and an optical fiber coupling output laser 5;
the side surface of the detection tank 14 is provided with a sample inlet and a sample outlet; the sample inlet is connected with the water outlet 13; the sample outlet is connected with the input end of the peristaltic pump 16; the output end of the peristaltic pump 16 is connected with a second return pipe 17;
the digital camera 15 and the optical fiber coupling output laser 5 are arranged oppositely, so that the laser beam emitted by the optical fiber coupling output laser 5 is normally incident on the image sensor of the digital camera 15 through the detection pool 14;
the detection device further comprises a power supply system, a switch circuit, a remote control signal receiving module 7 and a remote controller;
the power supply system comprises a battery 1, a first voltage regulating module 3 and a second voltage regulating module 4;
the switching circuit comprises a first switching circuit 2, a second switching circuit 6 and a third switching circuit 9;
the battery 1 is respectively connected with one end of the first switch circuit 2, one end of the second switch circuit 6 and one end of the third switch circuit 9;
the other end of the first switch circuit 2 is connected with the optical fiber coupling output laser 5 through the first voltage regulating module 3 and is connected with the digital camera 15 through the second voltage regulating module 4;
the other end of the second switch circuit 6 is connected with the peristaltic pump 16;
the other end of the third switch circuit 9 is connected with the self-priming pump 11;
the switch of the first switch circuit 2 is a inching switch;
the switches of the second switch circuit 6 and the third switch circuit 9 are delay switches;
the remote control signal receiving module 7 is respectively connected with the control end of the first switch circuit 2, the control end of the second switch circuit 6, the control end of the third switch circuit 9 and the shutter switch of the digital camera 15;
the method comprises the following steps:
the third switch circuit 9 is controlled to be turned on, the water intake head 10 is controlled to absorb the water sample on the water surface through the self-priming pump 11, and the water sample is conveyed into the transfer device 18;
the first switch circuit 2 is controlled to be turned on, so that the digital camera 15 and the optical fiber coupling output laser 5 are started;
controlling the second switch circuit 6 to be turned on, controlling the water sample in the shifter 18 to be shifted into the detection tank 14 through the peristaltic pump 16, and circularly discharging the water sample in the detection tank 14;
controlling the digital camera 15 to shoot and record a digital hologram;
repeating the steps of controlling the second switch circuit 6 to be turned on, controlling the water sample in the shifter 18 to be shifted into the detection tank 14 through the peristaltic pump 16, circularly discharging the water sample in the detection tank 14 until the step of controlling the digital camera 15 to shoot and record a digital hologram until the whole detection of the water sample in the shifter 18 is completed;
and controlling the first switch circuit 2 to be closed, so that the digital camera 15 and the optical fiber coupling output laser 5 are shut down.
The third switch circuit 9 is controlled to be turned on, the water intake head 10 is controlled to absorb the water sample on the water surface through the self-priming water pump 11, and the water sample is conveyed into the transfer device 18, and the method specifically comprises the following steps:
setting the delay time of automatic closing after the third switch circuit 9 is turned on as the ratio of the volume of the shifter 18 to the flow rate of the self-priming water pump 11;
an on control instruction is issued to the third switch circuit 9.
The control of the second switch circuit 6 is conducted, the peristaltic pump 16 controls the water sample in the shifter 18 to be shifted into the detection tank 14, and the water sample in the detection tank 14 is circularly discharged, and the control method specifically comprises the following steps:
setting the delay time of the automatic closing after the second switch circuit 6 is turned on as the ratio of the volume of the detection cell 14 to the flow rate of the peristaltic pump 16;
an on control command is issued to the second switching circuit 6.
According to the specific embodiment provided by the invention, the water surface plankton detection device and method also have the following technical effects:
the water taking head 10 provided by the invention is designed into an annular structure, the inner side of the water taking head is provided with a sealed floating pipe 22, and the outer side of the water taking head is provided with a water taking pipe 23. The outside of the water intake pipe 23 is provided with a row of water intake holes 21 and a water intake interface 24. Because the floating pipe 22 and the water intake pipe 23 are made of soft materials, when the water intake head 10 is placed on the water surface, the water intake hole 21 on the outer side of the water intake pipe 23 is just positioned at the water surface along with the fluctuation of the water surface, and only the water sample on the water surface is ensured to be pumped during pumping. Has higher detection efficiency on plankton living on the surface of water.
The present invention is also provided with a diverter 18 for removing bubbles generated when the self-priming pump 11 pumps surface water. The transfer device 18 is provided with a water inlet, an air outlet 8 and a water outlet 13, wherein the water inlet 19 and the air outlet 8 are arranged at the top of the container, the water outlet 13 is arranged at the bottom of the container, and the air outlet 8 is used for discharging bubbles and redundant water sample. The water sample from which the bubbles are removed enters the detection tank 14 from the water outlet 13 of the shifter 18 through the water pipe for digital holographic imaging detection, and the imaging process has no interference of a filter screen, thereby being beneficial to the subsequent image recognition and improving the detection accuracy.
The invention combines the coaxial digital holographic imaging technology with the remote control technology, utilizes the remote control signal to control the switch of the self-priming pump 11 and the peristaltic pump 16 in the sampling system, and then utilizes the remote control signal to control the switch of the laser and the digital camera in the digital holographic imaging system after extracting the water sample, simultaneously, the shutter of the remote control digital camera records the digital hologram, and the digital hologram is directly recorded after the field sampling, and the detection of the plankton on the water surface can be realized only by outputting the recorded digital hologram to a computer for digital holographic reconstruction. The water sample is not taken back to a laboratory, so that the whole water sample detection process is more intelligent, and the detection labor intensity is reduced.
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present invention and the core ideas thereof; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (10)
1. A water plankton detection device is characterized in that,
the detection device comprises a sampling system and a detection system;
the sampling system comprises a water taking head, a self-priming water pump and a transfer device;
the water taking head comprises a floating pipe and a water taking pipe;
the floating pipe and the water intake pipe are annular hollow hoses, and the water intake pipe is fixedly stuck to the outer side of the floating pipe; the floating pipe is used for floating the water taking head on the water surface under the action of buoyancy;
the outer side of the water intake pipe is provided with a plurality of water inlets and an interface;
the interface is connected with a water inlet pipe of the self-priming water pump;
the transfer device is connected with a water outlet pipe of the self-priming water pump; the self-priming water pump is used for controlling the water taking head to absorb a water sample on the water surface through the water inlet hole and conveying the water sample into the transfer device;
the transfer device is connected with the detection system and is used for separating bubbles in the water sample and conveying the separated water sample to the detection device for detection.
2. A water surface plankton detection device according to claim 1, wherein,
the shifter is provided with a water inlet, an air outlet and a water outlet;
the water inlet and the air outlet are both arranged at the top of the shifter;
the water outlet is arranged at the bottom of the shifter;
the water inlet is connected with a water outlet pipe of the self-priming water pump;
the exhaust port is connected with the first return pipe and is used for exhausting bubbles in the water sample and exhausting the water sample in the transfer device;
the water outlet is connected with the detection system through a hose.
3. A water surface plankton detection device according to claim 2, wherein,
the detection system comprises a detection cell and a peristaltic pump;
the detection pool is of a closed square structure;
the side surface of the detection tank is provided with a sample inlet and a sample outlet;
the sample inlet is connected with the water outlet;
the sample outlet is connected with the input end of the peristaltic pump;
the output end of the peristaltic pump is connected with a second return pipe;
the peristaltic pump is used for controlling the water sample in the transfer device to be transferred into the detection tank for detection, and the water sample in the detection tank is circulated into the water through the second return pipe.
4. A water surface plankton detection device according to claim 3, wherein,
the detection system also comprises a digital camera and an optical fiber coupling output laser;
the digital camera and the optical fiber coupling output laser are arranged oppositely, so that laser beams emitted by the optical fiber coupling output laser are normally incident on an image sensor of the digital camera through a detection pool;
the digital camera is used for shooting and recording digital holograms.
5. A water surface plankton detection device according to claim 4, wherein,
the detection device also comprises a power supply system and a switch circuit;
the power supply system comprises a battery, a first voltage regulating module and a second voltage regulating module;
the switching circuit comprises a first switching circuit, a second switching circuit and a third switching circuit;
the battery is respectively connected with one end of the first switch circuit, one end of the second switch circuit and one end of the third switch circuit;
the other end of the first switch circuit is connected with the optical fiber coupling output laser through the first voltage regulating module and connected with the digital camera through the second voltage regulating module; the first voltage regulating module is used for converting the battery voltage into the voltage required by the optical fiber coupling output laser; the second voltage regulating module is used for converting the battery voltage into the voltage required by the digital camera;
the other end of the second switch circuit is connected with the peristaltic pump;
the other end of the third switch circuit is connected with the self-priming pump.
6. A water surface plankton detection device according to claim 5, wherein,
the switch of the first switch circuit is a inching switch;
the switches of the second switch circuit and the third switch circuit are delay switches.
7. A water surface plankton detection device according to claim 5, wherein,
the detection device also comprises a remote control signal receiving module and a remote controller;
the remote control signal receiving module is respectively connected with the control end of the first switch circuit, the control end of the second switch circuit, the control end of the third switch circuit and the shutter switch of the digital camera;
the remote controller is used for sending a remote control signal to the remote control signal receiving module;
the remote control signal receiving module is used for controlling corresponding circuit actions according to the remote control signals.
8. A method for detecting plankton on a water surface, which is applied to a device for detecting plankton on a water surface, the device comprising: a sampling system and a detection system;
the sampling system comprises a water taking head, a self-priming water pump and a transfer device;
the water taking head comprises a floating pipe and a water taking pipe;
the floating pipe and the water intake pipe are annular hollow hoses, and the water intake pipe is fixedly stuck to the outer side of the floating pipe; the outer side of the water intake pipe is provided with a plurality of water inlets and an interface;
the interface is connected with a water inlet pipe of the self-priming water pump; the transfer device is connected with a water outlet pipe of the self-priming water pump; the transfer device is connected with the detection system;
the detection system comprises a detection pool, a peristaltic pump, a digital camera and an optical fiber coupling output laser;
the side surface of the detection tank is provided with a sample inlet and a sample outlet; the sample inlet is connected with the water outlet; the sample outlet is connected with the input end of the peristaltic pump; the output end of the peristaltic pump is connected with a second return pipe;
the digital camera and the optical fiber coupling output laser are arranged oppositely, so that laser beams emitted by the optical fiber coupling output laser are normally incident on an image sensor of the digital camera through a detection pool;
the detection device also comprises a power supply system, a switch circuit, a remote control signal receiving module and a remote controller;
the power supply system comprises a battery, a first voltage regulating module and a second voltage regulating module;
the switching circuit comprises a first switching circuit, a second switching circuit and a third switching circuit;
the battery is respectively connected with one end of the first switch circuit, one end of the second switch circuit and one end of the third switch circuit;
the other end of the first switch circuit is connected with the optical fiber coupling output laser through the first voltage regulating module and connected with the digital camera through the second voltage regulating module;
the other end of the second switch circuit is connected with the peristaltic pump;
the other end of the third switch circuit is connected with the self-priming pump;
the switch of the first switch circuit is a inching switch;
the switches of the second switch circuit and the third switch circuit are delay switches;
the remote control signal receiving module is respectively connected with the control end of the first switch circuit, the control end of the second switch circuit, the control end of the third switch circuit and the shutter switch of the digital camera;
the method comprises the following steps:
the third switch circuit is controlled to be turned on, the water sampling head is controlled to absorb the water sample on the water surface through the self-priming pump, and the water sample is conveyed into the transfer device;
controlling the first switch circuit to be turned on to start the digital camera and the optical fiber coupling output laser;
controlling a second switch circuit to be switched on, controlling the water sample in the shifter to be shifted into a detection pool through a peristaltic pump, and circularly discharging the water sample in the detection pool;
controlling the digital camera to shoot and record a digital hologram;
repeating the steps of controlling the second switch circuit to be turned on, transferring the water sample in the transfer device to a detection pool through peristaltic pump control, circularly discharging the water sample in the detection pool until the step of controlling the digital camera to shoot and record a digital hologram until the water sample in the transfer device is completely detected;
and controlling the first switch circuit to be closed, so that the digital camera and the optical fiber coupling output laser are powered off.
9. A method for detecting plankton on water according to claim 8, wherein,
the third switch circuit is controlled to be connected, the water sample on the water surface is sucked by the self-priming pump control water suction head, and the water sample is conveyed into the transfer device, and the method specifically comprises the following steps:
setting the delay time of automatic closing after the third switch circuit is turned on as the ratio of the volume of the transfer device to the flow rate of the self-priming water pump;
and sending an on control instruction to the third switch circuit.
10. A method for detecting plankton on water according to claim 8, wherein,
the control second switch circuit is connected, the water sample in the shifter is shifted into the detection pool through peristaltic pump control, and the water sample in the detection pool is circularly discharged, and the control second switch circuit specifically comprises:
setting the delay time of automatic closing after the second switch circuit is switched on as the ratio of the volume of the detection pool to the flow rate of the peristaltic pump;
and sending an on control instruction to the second switch circuit.
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