CN110092847A - A method of extracting Polysaccharides in Bamboo Leaves - Google Patents
A method of extracting Polysaccharides in Bamboo Leaves Download PDFInfo
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
A method of extracting Polysaccharides in Bamboo Leaves, it is first extracted or is extracted with organic solvent and remove fat-soluble coloring matter in dry bamboo leaf powder, secondly water is added, cellulase and hemicellulase is added in a mild condition again, then enzymatic hydrolysis mixed liquor is smashed to pieces with tissue mashing machine, be finally separated by solid-liquid separation the clear solution for obtaining high Polysaccharides in Bamboo Leaves content.Polysaccharides in Bamboo Leaves is extracted using method of the invention, compared with high temperature water leaching, there is saving heat energy and improve the advantages such as efficiency;With ultrasonic wave and microwave-assisted extraction and supercritical fluid process extraction phase ratio, there are the advantages such as low equipment requirement, simple process and efficiency height;Compared with digesting Assisted Extraction, with the advantage that cellulase and hemicellulose enzyme dosage are low and extraction efficiency is high.
Description
Technical field
The present invention relates to a kind of extracting method of water-soluble Polysaccharides in Bamboo Leaves, in particular to a kind of cellulase and hemicellulose
The method of water-soluble polysaccharide in the enzyme assisted extraction leaf of bamboo, belongs to polysaccharide functional component extractive technique field in natural products.
Background technique
China possesses more than 500 bamboos, and cultivated area is more than 5,000,000 hectares, is mainly distributed on Hainan, Yunnan, Guangdong, wide
The torrid zone such as west, Fujian, Hunan, Hubei, Zhejiang, Jiangsu, Jiangxi and Sichuan or subtropical zone province.Because having good heat-clearing solution
Malicious effect, the leaf of bamboo have long edible medicinal history.Modern study also shows that Polysaccharides in Bamboo Leaves has anticancer activity, antioxidation
And the effects of immunological regulation, therefore extracting and developing about Polysaccharides in Bamboo Leaves and purifying research increase year by year.Because obtained more
Sugared content is low, purity of polysaccharide is not high and extraction mixture comprehensive performance difference etc., so as the byproduct during bamboo processing,
The leaf of bamboo is that major part is not fully utilized as waste still.
The extracting method of Polysaccharides in Bamboo Leaves predominantly has solvent extraction method, microwave and ultrasound secondary solvent extraction, enzyme to urge
Change edman degradation Edman and supercritical fluid extraction etc..Solvent extraction method extracts the with a long history of Polysaccharides in Bamboo Leaves, traditional boiling water decoction
It is exactly a kind of solvent extraction method of classics, advantage includes that equipment requirement is lower, process flow is simple, cost consumption is few, but because more
Sugared recovery rate is low and it is made to lose industrialization value.For the recovery rate for improving Polysaccharides in Bamboo Leaves, optimize extracting technology condition and increase
Extracting times are effective methods, but also reduce the efficiency of extraction simultaneously.Microwave and ultrasound secondary solvent extraction
The extraction efficiency that Polysaccharides in Bamboo Leaves can be greatly improved is ruptured using the microwave assisted plant cell of high-frequency electromagnetic wave technology and is improved
The extraction efficiency of Polysaccharides in Bamboo Leaves improves middle dissolution of the Polysaccharides in Bamboo Leaves in solvent using the ultrasonic technology for generating cavitation
Rate.Microwave and ultrasound Assisted Extraction technology extraction time is short, extracting efficiency is high, extraction solvent is few and leaching liquor in polysaccharide
Content is high and reduces subsequent concentrated cost, but ultrasonic wave and microwave technology all have high equipment cost and possible leakage
Microwave and ultrasound protects the deficiency of difficult aspect and is rarely used in the polysaccharide in the assisted extraction leaf of bamboo in the industry.Enzymatic is decomposed
Or the tissue such as dispersion leaf of bamboo cell wall, ensure the content that Polysaccharides in Bamboo Leaves in leaching liquor is improved under the conditions of relatively mild, difficult point
Optimization, substrate and the extract of the screening and preferred, enzyme cost high, enzymatic hydrolysis most suitable process conditions that are enzyme class are to enzyme
Active inhibition and the efficiently holding etc. of enzymatic activity.It is the Co 2 supercritical fluid technology of entrainer using water or ethyl alcohol
Supercritical fluid technique can increase substantially extraction efficiency and extract the physiologically active of polysaccharide, but the device is complicated, operation
The reasons such as pressure is big and operating cost is high limit its extensive industrialization.Though the efficiency of homogenate extraction is high, it there is no and efficiently mention
Take the report of Polysaccharides in Bamboo Leaves;The extraction liquid measure that extrusion directly squeezes fresh leaf of bamboo acquisition is very little, polyoses content therein
Less.The total starches content that above-mentioned all methods extract the leaf of bamboo is low, mainly by soluble polysaccharide molecular weight in the leaf of bamboo greatly and
Caused by content is low, furthermore the factors such as high leaf of bamboo content of cellulose and glycoprotein also hinder the extraction of leaf of bamboo soluble polysaccharide.
Summary of the invention
It is an object of the invention to solve high temperature water logging leaf of bamboo soluble polysaccharide, to extract energy consumption high, ultrasonic wave and microwave-assisted
The equipment and complex process of leaf of bamboo soluble polysaccharide are extracted, water or ethyl alcohol are that the supercritical fluid technique extraction leaf of bamboo of entrainer can
The deficiencies of the device is complicated for soluble polysaccharide, operating pressure is big and operating cost is high;Solve high temperature flooding, ultrasonic wave auxiliary mentions
It takes, the problem that microwave radiation exaraction is low with conventional enzyme assisted extraction leaf of bamboo soluble polysaccharide recovery rate, and provides a kind of water-soluble
The highly effective extraction method of property Polysaccharides in Bamboo Leaves.
The present invention solves the technical solution that above-mentioned technical problem uses are as follows:
A method of extracting Polysaccharides in Bamboo Leaves, comprising the following steps:
The fresh leaf of bamboo is first dried, dried or dried by step 1, and then crushing is the bamboo leaf powder not less than 50 mesh, then uses petroleum
The extraction of the organic solvents such as ether, hexamethylene, n-hexane or ethyl alcohol or extrct remove the dark ingredient such as lutein, chlorophyll and polyphenol,
Organic solvent is evaporated off;Wherein extraction removes the process conditions of dark ingredient are as follows: with 10 ~ 20 times of quality of petroleum of bamboo leaf powder
Ether, hexamethylene, n-hexane or ethyl alcohol, extracted under 30 DEG C to organic solvent boiling temperature 1 ~ 3 removal lutein, chlorophyll and
The dark color ingredient such as polyphenol;Wherein extraction process removes the process conditions of dark ingredient are as follows: with 20 ~ 30 times of quality of petroleum of bamboo leaf powder
The organic solvents such as ether, hexamethylene, n-hexane or ethyl alcohol extract removal lutein, chlorophyll and more under organic solvent boiling temperature
The dark color ingredient such as phenol.
The mass ratio of step 2, the leaf of bamboo residue for the above-mentioned removal organic solvent that is soaked in water, water and bamboo leaf powder is (8 ~ 20):
1, the temperature of immersion is 20 ~ 40 DEG C, and the time of immersion is 0.5h ~ 2h.
Step 3,0.05% ~ 0.3% enzymatic activity that bamboo leaf powder quality is added in the bamboo leaf powder that water impregnates are not less than
0.05% ~ 0.2% enzymatic activity of 5000u/g cellulase and bamboo leaf powder quality is not less than 5000u/g hemicellulase and stirs
Uniformly.
Step 4, the bamboo leaf powder mixed liquor that cellulase and hemicellulase is added digest 2 h ~ 10 at 30 DEG C ~ 45 DEG C
h。
Step 5, with tissue mashing machine or homogenate crusher machine enzymolysis liquid leaf of bamboo cell.
Step 6 is separated step 5 leaf of bamboo residue with liquid with the methods of filtering or centrifugation, is obtained containing soluble Polysaccharides in Bamboo Leaves
It is faint yellow to yellow solution.
Step 7 measures the faint yellow soluble polysaccharide into yellow solution with sulfuric acid-phynol method using glucose as reference substance
Content, and calculate the soluble polysaccharide content in dry bamboo leaf powder, the i.e. recovery rate of soluble polysaccharide in bamboo leaf powder.
The present invention have be not limited to it is following the utility model has the advantages that
(1) efficiency that the present invention extracts soluble polysaccharide in the leaf of bamboo is significantly higher than conventional high-temperature water leaching, ultrasonic wave auxiliary
Water leaching, microwave-assisted water leaching, enzymatic hydrolysis Assisted Extraction technique and water or ethyl alcohol are the carbon dioxide of entrainer
Supercritical fluid extraction technique;
(2) cost that the present invention extracts soluble polysaccharide in the leaf of bamboo is substantially less than high temperature water leaching, ultrasonic wave auxiliary water logging
Mention that technique, microwave-assisted water leaching, carbon dioxide that enzymatic hydrolysis Assisted Extraction technique and water or ethyl alcohol are entrainer are super to be faced
Boundary's fluid extraction technique;
(3) low energy consumption for the technique higher temperatures water leaching of the invention for extracting soluble polysaccharide in the leaf of bamboo, and polysaccharide extract rate is high;
(4) present invention extract the leaf of bamboo in soluble polysaccharide equipment compared with Ultrasonic Wave-Assisted Extraction, it is microwave-assisted extraction and it is overcritical
The equipment of fluid extraction is simple;
(5) present invention extracts the technique of soluble polysaccharide in the leaf of bamboo compared with Ultrasonic Wave-Assisted Extraction technique, microwave-assisted extracting technology
With supercritical fluid extraction simple process.
Specific embodiment
The present invention is further explained in the light of specific embodiments, but is not limited in any way the present invention
System, based on present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.
Embodiment 1
Summer Yixing moso bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, 40
1 h, centrifuge separation are extracted with 200 g hexamethylenes at DEG C;It is extracted and is centrifugated at 40 DEG C in two times with 320g hexamethylene again
Solid residue;The cyclohexane solution for being centrifugated acquisition three times is collected, evaporation recycling cyclohexane solvent simultaneously obtains lutein, Ye Lv
The dark mixtures such as element and polyphenol.Drying extracts the leaf of bamboo residue for removing dark matter through hexamethylene, 200g water is added, at 30 DEG C
1h is impregnated under lower stirring;Adding cellulase that 0.05 g enzymatic activity is 10000 u/g and 0.03 g enzymatic activity is 18000
The hemicellulase of u/g continues 3 h of stirring enzymatic hydrolysis at 35 DEG C, and centrifuge separation obtains after smashing accumulative 3min to pieces with tissue mashing machine
Weak yellow liquid is obtained, with soluble Polysaccharides in Bamboo Leaves content in Phenol-sulphate acid method measurement liquid, calculates mentioning for leaf of bamboo soluble polysaccharide
Taking rate is 6.6 %.
Embodiment 2
The winter Ningbo side bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20 g fine powders are weighed, are used
320 g n-hexanes extract 24 h, the solid was filtered residue;Extract is collected, evaporation recycling n-hexane solvent obtains lutein, leaf
The yellow mixture of green element and polyphenol.The leaf of bamboo residue extracted through n-hexane is dried, 320g water is added, is slowly stirred at 50 DEG C
1 h of lower immersion;Add the cellulase that 0.05g enzymatic activity is 8000u/g and the half fiber that 0.04 g enzymatic activity is 8000 u/g
Plain enzyme is tieed up, continuation digests 3 h at 45 DEG C, and centrifuge separation obtains weak yellow liquid after smashing accumulative 3min to pieces with tissue mashing machine,
With soluble Polysaccharides in Bamboo Leaves content in Phenol-sulphate acid method measurement liquid, calculate that the soluble polysaccharide of the dry square bamboo leaf of bamboo in winter Ningbo mentions
Taking rate is 6.4%.
Embodiment 3
The summer Wuxi moso bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, 70
3 h, centrifuge separation are extracted with 360 g dehydrated alcohols at DEG C, then extraction centrifugation divides at 70 DEG C in two times with 400 g dehydrated alcohols
From solid residue;The ethanol solution being centrifugally separating to obtain three times is collected, evaporation recycling anhydrous ethanol solvent simultaneously obtains leaf
The mixture of flavine, chlorophyll and polyphenol.The leaf of bamboo residue extracted through 3 dehydrated alcohols is dried, 320g water is added, at 35 DEG C
Stirring is lower to impregnate 1 h;Adding cellulase that 0.04 g enzymatic activity is 18000 u/g and 0.03 g enzymatic activity is 18000 u/
The hemicellulase of g, 8 h of stirring enzymatic hydrolysis at 35 DEG C, centrifuge separation obtains yellowish after smashing accumulative 3 min to pieces with tissue mashing machine
Color liquid calculates that the solubility of the Summer Dry moso bamboo leaf of bamboo is more with soluble Polysaccharides in Bamboo Leaves content in Phenol-sulphate acid method measurement liquid
The recovery rate of sugar is 4.8%.
Embodiment 4
The red shell bamboo leaf of bamboo of the report for Ningbo in Spring of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed,
1 h, centrifuge separation are extracted with 260 g petroleum ethers at 50 DEG C;It is extracted at 50 DEG C with 220g petroleum ether again and is centrifugated to obtain solid
Residue;The petroleum ether alkane solution for being centrifugated acquisition twice is collected, evaporation recycling petroleum ether solvent simultaneously obtains lutein, chlorophyll
With the dark mixtures such as polyphenol.Drying extracts the leaf of bamboo residue for removing dark matter through petroleum ether, and 260g water is added, slow at 30 DEG C
Slow stirring is lower to impregnate 50 min;It adds cellulase that 0.06 g enzymatic activity is 10000 u/g and 0.03 g enzymatic activity is
The hemicellulase of 18000 u/g continues 6 h of stirring enzymatic hydrolysis at 40 DEG C, is centrifuged after smashing accumulative 3min to pieces with tissue mashing machine
Separation obtains weak yellow liquid, with soluble Polysaccharides in Bamboo Leaves content in Phenol-sulphate acid method measurement liquid, calculates that the leaf of bamboo is soluble more
The recovery rate of sugar is 6.3 %.
Embodiment 5
The winter Ningbo Huang shell bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, is used
280 g dehydrated alcohols extract 24 h, evaporation extract recycling alcohol solvent and to obtain lutein, chlorophyll and polyphenol etc. dark mixed
Close object.Drying extracts the leaf of bamboo residue for removing dark matter through dehydrated alcohol, and 340 g water are added, and impregnates under stirring at 40 DEG C
1.5h;Add the cellulase that 0.02 g enzymatic activity is 18000 u/g and the hemicellulose that 0.04 g enzymatic activity is 18000 u/g
Plain enzyme continues 6 h of stirring enzymatic hydrolysis at 40 DEG C, and centrifuge separation obtains weak yellow liquid after being homogenized 5 min with refiner, uses sulfuric acid
Phynol method measures soluble Polysaccharides in Bamboo Leaves content in liquid, calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 6.0 %.
Embodiment 6
The summer Ningbo eel bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, with 200
G dehydrated alcohol extracts 24 h, evaporation extract recycling alcohol solvent and the dark mixing such as obtain lutein, chlorophyll and polyphenol
Object.Drying extracts the leaf of bamboo residue for removing dark matter through dehydrated alcohol, and 280 g water are added, and impregnates 1h under stirring at 35 DEG C;
The cellulase that 0.02 g enzymatic activity is 18000 u/g and the hemicellulase that 0.04 g enzymatic activity is 18000 u/g are added,
Continue 7 h of stirring enzymatic hydrolysis at 45 DEG C, centrifuge separation obtains weak yellow liquid after being homogenized 5 min with refiner, uses phenol sulfuric acid
Method measures soluble Polysaccharides in Bamboo Leaves content in liquid, calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 7.7 %.
Embodiment 7
The winter Ningbo bitter bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, is used
300g dehydrated alcohol extracts 24 h, evaporation extract recycling alcohol solvent and to obtain lutein, chlorophyll and polyphenol etc. dark mixed
Close object.Drying extracts the leaf of bamboo residue for removing dark matter through dehydrated alcohol, and 200 g water are added, and impregnates under stirring at 40 DEG C
0.5h;Add the cellulase that 0.02 g enzymatic activity is 18000 u/g and the hemicellulose that 0.03 g enzymatic activity is 18000 u/g
Plain enzyme continues 9 h of stirring enzymatic hydrolysis at 45 DEG C, and centrifuge separation obtains weak yellow liquid after being homogenized 5 min with refiner, uses sulfuric acid
Phynol method measures soluble Polysaccharides in Bamboo Leaves content in liquid, calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 6.4 %.
Embodiment 8
The winter Ningbo thunder bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, with 260
G dehydrated alcohol extracts 24 h, evaporation extract recycling alcohol solvent and the dark mixing such as obtain lutein, chlorophyll and polyphenol
Object.Drying extracts the leaf of bamboo residue for removing dark matter through dehydrated alcohol, and 200 g water are added, and impregnates under stirring at 40 DEG C
1.6h;Add the cellulase that 0.04 g enzymatic activity is 18000 u/g and the hemicellulose that 0.03 g enzymatic activity is 18000 u/g
Plain enzyme continues 4 h of stirring enzymatic hydrolysis at 45 DEG C, and centrifuge separation obtains weak yellow liquid after being homogenized 5 min with refiner, uses sulfuric acid
Phynol method measures soluble Polysaccharides in Bamboo Leaves content in liquid, calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 5.5 %.
Embodiment 9
The autumn Ningbo dragon bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, with 220
G dehydrated alcohol extracts 24 h, evaporation extract recycling alcohol solvent and the dark mixing such as obtain lutein, chlorophyll and polyphenol
Object.Drying extracts the leaf of bamboo residue for removing dark matter through dehydrated alcohol, and 220 g water are added, and impregnates 2 under stirring at 30 DEG C
h;Add the cellulase that 0.02 g enzymatic activity is 18000 u/g and the hemicellulose that 0.02 g enzymatic activity is 18000 u/g
Enzyme continues 9 h of stirring enzymatic hydrolysis at 45 DEG C, and centrifuge separation obtains weak yellow liquid after being homogenized 5 min with refiner, with sulfuric acid benzene
Phenol method measures soluble Polysaccharides in Bamboo Leaves content in liquid, calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 5.3 %.
Embodiment 10
The summer Ningbo moso bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, with 220
G dehydrated alcohol extracts 24 h, evaporation extract recycling alcohol solvent and the dark mixing such as obtain lutein, chlorophyll and polyphenol
Object.Drying extracts the leaf of bamboo residue for removing dark matter through dehydrated alcohol, and 320 g water are added, and impregnates 1h under stirring at 40 DEG C;
The cellulase that 0.02 g enzymatic activity is 18000 u/g and the hemicellulase that 0.02g enzymatic activity is 18000 u/g are added,
Continue 4 h of stirring enzymatic hydrolysis at 40 DEG C, centrifuge separation obtains weak yellow liquid after being homogenized 5 min with refiner, uses phenol sulfuric acid
Method measures soluble Polysaccharides in Bamboo Leaves content in liquid, calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 4.7 %.
Comparative example 1
Summer Yixing moso bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, 40
1 h, centrifuge separation are extracted with 200 g hexamethylenes at DEG C;It is extracted and is centrifugated at 40 DEG C in two times with 320g hexamethylene again
Solid residue;The cyclohexane solution for being centrifugated acquisition three times is collected, evaporation recycling cyclohexane solvent simultaneously obtains lutein, Ye Lv
The dark mixtures such as element and polyphenol.Drying extracts the leaf of bamboo residue for removing dark matter through hexamethylene, and 400g water is added, heats back
24 h of stream extraction, are centrifugated after cooling and obtain the deep yellow color liquid containing soluble Polysaccharides in Bamboo Leaves, measure liquid with Phenol-sulphate acid method
Middle solubility Polysaccharides in Bamboo Leaves content calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 1.7 %.
Comparative example 2
Summer Yixing moso bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, 40
1 h, centrifuge separation are extracted with 200 g hexamethylenes at DEG C;It is extracted and is centrifugated at 40 DEG C in two times with 320g hexamethylene again
Solid residue;The cyclohexane solution for being centrifugated acquisition three times is collected, evaporation recycling cyclohexane solvent simultaneously obtains lutein, Ye Lv
The dark mixtures such as element and polyphenol.Drying extracts the leaf of bamboo residue for removing dark matter through hexamethylene, 300g water is added, at 30 DEG C
The interval that lower is 350 W with power and frequency is 20 KHz uses Ultrasonic Wave-Assisted Extraction Polysaccharides in Bamboo Leaves, uses ultrasonic-leaching
Cumulative time be 4 h.Filtering obtains the pale yellow filtrate containing soluble polysaccharide, with soluble bamboo in Phenol-sulphate acid method measurement filtrate
Leaf polyose content calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 3.4 %.
Comparative example 3
Summer Yixing moso bamboo leaf of bamboo of drying is crushed, filters out fine powder with 50 the polished standard screen.20g fine powder is weighed, 40
1 h, centrifuge separation are extracted with 200 g hexamethylenes at DEG C;It is extracted and is centrifugated at 40 DEG C in two times with 320g hexamethylene again
Solid residue;The cyclohexane solution for being centrifugated acquisition three times is collected, evaporation recycling cyclohexane solvent simultaneously obtains lutein, Ye Lv
The dark mixtures such as element and polyphenol.Drying through hexamethylene extract remove dark matter leaf of bamboo residue, be added 300g water, then plus
Heat reflux 24 h of extraction, after smashing accumulative 3 min to pieces with tissue mashing machine, centrifuge separation, measurement centrifuge separation obtains can in liquid
Dissolubility Polysaccharides in Bamboo Leaves content calculates that the recovery rate of leaf of bamboo soluble polysaccharide is 2.9 %.
Claims (8)
1. a kind of method for extracting Polysaccharides in Bamboo Leaves, which comprises the following steps:
(1) first the fresh leaf of bamboo is dried, dried or dried, then crushed and obtain bamboo leaf powder, by obtained bamboo leaf powder with organic molten
Agent extraction or extracting remove colored component therein;
(2) coloured with having been removed in 10 times ~ 20 times of bamboo leaf powder quality of water dispersion and soaking step (1) at 20 DEG C ~ 40 DEG C
0.5 h of bamboo leaf powder residue ~ 2h of ingredient;
(3) cellulase of the % of 0.05 % ~ 0.3 of bamboo leaf powder quality and the hemicellulase of the % of 0.05 % ~ 0.2 is added, then
The h of 2 h ~ 10 is digested at 30 DEG C ~ 45 DEG C;
(4) enzymolysis liquid leaf of bamboo cell is crushed with Mechanical Method;
(5) mixed liquor after smudge cells is separated by solid-liquid separation, is obtained faint yellow to yellow solution containing soluble Polysaccharides in Bamboo Leaves.
2. a kind of method for extracting Polysaccharides in Bamboo Leaves according to claim 1, it is characterised in that: organic described in (1) step
Solvent is petroleum ether, hexamethylene, n-hexane or ethyl alcohol.
3. a kind of method for extracting Polysaccharides in Bamboo Leaves according to claim 1, it is characterised in that: extraction described in (1) step
Process conditions are as follows: extracting times are 1 ~ 3 time, and the mass ratio of the organic solvent extracted every time and initial bamboo leaf powder is (10 ~ 20):
1, temperature is 30 DEG C to organic solvent boiling point.
4. a kind of method for extracting Polysaccharides in Bamboo Leaves according to claim 1, it is characterised in that: extracting described in (1) step
Process conditions are as follows: the mass ratio of organic solvent and bamboo leaf powder is (20 ~ 30): 1, the temperature of extracting is the boiling point of solvent, extracting
Time 10 h ~ 48 h.
5. a kind of method for extracting Polysaccharides in Bamboo Leaves according to claim 1, it is characterised in that: cellulose described in (3) step
The enzymatic activity of enzyme is not less than 5000 u/g, and the enzymatic activity of the hemicellulase is not less than 5000 u/g.
6. a kind of method for extracting Polysaccharides in Bamboo Leaves according to claim 1, it is characterised in that: enzymatic hydrolysis described in (3) step
Condition are as follows: reaction temperature be 36 DEG C ~ 42 DEG C, the reaction time be the h of 2 h ~ 4.
7. a kind of method for extracting Polysaccharides in Bamboo Leaves according to claim 1, it is characterised in that: machinery described in (4) step
The method that method is crushed enzymolysis liquid leaf of bamboo cell is historrhexis's method or homogenate method.
8. a kind of method for extracting Polysaccharides in Bamboo Leaves according to claim 1, it is characterised in that: solid-liquid described in (5) step
Isolated method is filtering or centrifugation.
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