CN102872201B - Method for extracting general flavones from folium mori - Google Patents
Method for extracting general flavones from folium mori Download PDFInfo
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- CN102872201B CN102872201B CN201210335925.2A CN201210335925A CN102872201B CN 102872201 B CN102872201 B CN 102872201B CN 201210335925 A CN201210335925 A CN 201210335925A CN 102872201 B CN102872201 B CN 102872201B
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Abstract
The invention discloses a method for extracting general flavones from folium mori, which comprises the following steps of: washing, drying and crushing the fresh folium mori, and screening the folium mori by a screen of 160 meshes to obtain the folium mori powder; and then uniformly mixing the folium mori powder and the purified water according to a certain ratio, extracting the mixed solution in a microwave extracting device, filtrating the mixed solution after the mixed solution is extracted after a while, performing constant volume, and detecting the content of the general flavones in the folium mori. According to the invention, the water as a solvent is combined with a microwave ultrasonic method to extract the general flavones for the first time. The method for extracting general flavones from folium mori provided by the invention has the advantages of fastness, high efficiency, high extraction ratio and environmental-friendly performance, and reduces the influence on the detection results caused by extracting the impurities such as chlorophyll through an organic solvent, thereby being more beneficial for the subsequent separation and purification work.
Description
Technical field
The present invention relates to a kind of natural plants effective active component extracting method, be specifically related to a kind of method of extracting total flavones from Folium Mori.
Background technology
Folium Mori have another name called " Herba adianti myriosori ", for Morus (
morus alba L) blade of plant, be one of conventional Chinese herbal medicine.Folium Mori total flavonoids is the main active in Folium Mori.Flavones in mulberry leaves constituents mainly comprises rutin, Quercetin, isoquercitin and dihydro Rhizoma Kaempferiae etc.In Folium Mori (dry weight), being about 2.53% left and right containing Flavonoids, is higher a kind of of all plant stem-leaf flavonoid contents.Modern pharmacology studies have shown that, flavones in mulberry leaves has the effects such as blood sugar lowering, antiinflammatory, antioxidation and blood fat reducing.
The extraction of Folium Mori total flavonoids is mainly to take alcoholic solution as solvent at present, and extraction method has ultrasonic auxiliary law, water bath reflux method, extraction and supercritical CO
2extraction method etc.There is long, the problem such as efficiency is low of extraction time in these methods, cannot meet the demand of people to the large production of modernization to some extent.Microwave-assisted extracts (Microwave-Assisted Extraction, MAE) and utilizes microwave heating to carry out the extraction process of accelerated solvent to target extract in solid sample.Its mechanism of action is that microwave radiation can make temperature in plant cell raise suddenly, makes its internal pressure surpass cell wall expansion ability and cause cell rupture, makes intracellular matter be delivered to around dissolved in solvent and extraction ratio is improved greatly.Water is to absorb the best medium of microwave, and water, as a kind of polar solvent, can effectively absorb microwave energy, promotes the swelling of cell wall to break, and improves the extraction efficiency of effective ingredients in plant.
And in prior art, do not using water as extracting the method for solvent in conjunction with microwave-assisted extraction.
Summary of the invention
In order to solve the above-mentioned technical problem existing in prior art, the object of the invention is to shorten the extraction time of Folium Mori total flavonoids, reduces environmental pollution and production cost, and a kind of method of extracting total flavones from Folium Mori of more efficient, environmental protection is provided.
In order to achieve the above object, the technical solution used in the present invention is as follows:
A method of extracting total flavones from Folium Mori, comprises the steps:
(1) new fresh mulberry leaf is cleaned, is dried, is pulverized rear 160 orders of crossing and obtain powder of Folium Mori;
(2) in powder of Folium Mori, add a certain amount of pure water, after mix homogeneously, sealing, put into Microwave Extraction Apparatus and carry out microwave-assisted extraction, extract once;
(3), after having extracted, by extracting liquid filtering, measure total flavones extraction ratio.
Further, after the middle Folium Mori of step (1) are clean, in 50 ℃ of oven dryings, make dried Folium Mori water content below 5%.
Further, in step (2), microwave power is 500W, extraction time 2～6min, and extracting temperature is 90～100 ℃.
Further, the ratio of the middle powder of Folium Mori of step (2) and pure water is at 1:25～1:30g/mL.
The Folium Mori extracting solution that adopts method of the present invention to obtain, the content of measuring total flavones with " sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry " reaches 2.22% left and right.
The advantage applies that the present invention has exists:
The present invention using water as extract solvent in conjunction with microwave-assisted extract Folium Mori total flavonoids compare with other method have fast, efficient, economize that solvent, selectivity are strong, advantages of environment protection.
The present invention is usingd water as extracting solvent, not only environmental protection, and greatly reduce the production cost that extracts Folium Mori total flavonoids; Under the effect of microwave, the cell wall rupture of Folium Mori, causes the abundant stripping of flavones ingredient to be diffused in solvent, thereby has improved extraction ratio; Microwave can destroy chlorophyllous structure simultaneously, avoids the impact of chlorophyll color when measuring light absorption value.
The invention provides the extracting method of total flavones in above-mentioned Folium Mori, can in preparing food, medicine, apply, reach the object that makes full use of Folium Mori resource.
Accompanying drawing explanation
Fig. 1 is for adopting the block diagram of the total flavones extraction ratio of Different Extraction Method.
The specific embodiment
Embodiment 1
The new fresh mulberry leaf of plucking is rinsed well, after naturally drying, put into baking oven, 50 ℃ are dried 12h, Folium Mori water content are down to below 5%.Dried Folium Mori are put into high speed disintegrator and pulverize, then cross 160 orders (sieve) and obtain powder of Folium Mori.Get 0.2g powder of Folium Mori and put into microwave extracting pipe, according to the ratio of 1:25g/mL, add pure water, then after sealing, put into Microwave Extraction Apparatus and extract, power is 500W, and extracting temperature is 95 ℃, and extraction time is 4min.After extraction completes, by extracting liquid filtering to 10mL volumetric flask, standardize solution, adopts " sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry " to measure extracting solution light absorption value at 510nm, and the extraction rate reached of calculating Folium Mori total flavonoids arrives 1.703%(with respect to the weight of powder of Folium Mori).
Embodiment 2
The new fresh mulberry leaf of plucking is rinsed well, after naturally drying, put into baking oven, 50 ℃ are dried 12h, Folium Mori water content are down to below 5%.Dried Folium Mori are put into high speed disintegrator and pulverize, then cross 160 orders and obtain powder of Folium Mori.Get 0.2g powder of Folium Mori and put into microwave extracting pipe, according to the ratio of 1:30g/mL, add pure water, then after sealing, put into Microwave Extraction Apparatus and extract, power is 500W, and extracting temperature is 95 ℃, and extraction time is 6min.After extraction completes, by extracting liquid filtering, to 10mL volumetric flask, standardize solution, adopts " sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry " to measure extracting solution light absorption value at 510nm, calculates the extraction rate reached to 2.018% of Folium Mori total flavonoids.
Embodiment 3
The new fresh mulberry leaf of plucking is rinsed well, after naturally drying, put into baking oven, 50 ℃ are dried 12h, Folium Mori water content are down to below 5%.Dried Folium Mori are put into high speed disintegrator and pulverize, then cross 160 orders and obtain powder of Folium Mori.Get 0.2g powder of Folium Mori and put into microwave extracting pipe, according to the ratio of 1:30g/mL, add pure water, then after sealing, put into Microwave Extraction Apparatus and extract, power is 500W, and extracting temperature is 100 ℃, and extraction time is 4min.After extraction completes, by extracting liquid filtering, to 10mL volumetric flask, standardize solution, adopts " sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry " to measure extracting solution light absorption value at 510nm, calculates the extraction rate reached to 1.902% of Folium Mori total flavonoids.
Embodiment 4
The new fresh mulberry leaf of plucking is rinsed well, after naturally drying, put into baking oven, 50 ℃ are dried 12h, Folium Mori water content are down to below 5%.Dried Folium Mori are put into high speed disintegrator and pulverize, then cross 160 orders and obtain powder of Folium Mori.Get 0.2g powder of Folium Mori and put into microwave extracting pipe, according to the ratio of 1:25g/mL, add pure water, then after sealing, put into Microwave Extraction Apparatus and extract, power is 500W, and extracting temperature is 90 ℃, and extraction time is 6min.After extraction completes, by extracting liquid filtering, to 10mL volumetric flask, standardize solution, adopts " sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry " to measure extracting solution light absorption value at 510nm, calculates the extraction rate reached to 1.828% of Folium Mori total flavonoids.
Embodiment 5
The new fresh mulberry leaf of plucking is rinsed well, after naturally drying, put into baking oven, 50 ℃ are dried 12h, Folium Mori water content are down to below 5%.Dried Folium Mori are put into high speed disintegrator and pulverize, then cross 160 orders and obtain powder of Folium Mori.Get 0.2g powder of Folium Mori and put into microwave extracting pipe, according to the ratio of 1:25g/mL, add pure water, then after sealing, put into Microwave Extraction Apparatus and extract, power is 500W, and extracting temperature is 100 ℃, and extraction time is 6min.After extraction completes, by extracting liquid filtering, to 10mL volumetric flask, standardize solution, adopts " sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry " to measure extracting solution light absorption value at 510nm, calculates the extraction rate reached to 2.141% of Folium Mori total flavonoids.
Embodiment 6
The new fresh mulberry leaf of plucking is rinsed well, after naturally drying, put into baking oven, 50 ℃ are dried 12h, Folium Mori water content are down to below 5%.Dried Folium Mori are put into high speed disintegrator and pulverize, then cross 160 orders and obtain powder of Folium Mori.Get 0.2g powder of Folium Mori and put into microwave extracting pipe, according to the ratio of 1:29g/mL, add pure water, then after sealing, put into Microwave Extraction Apparatus and extract, power is 500W, and extracting temperature is 100 ℃, and extraction time is 2min.After extraction completes, by extracting liquid filtering, to 10mL volumetric flask, standardize solution, adopts " sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry " to measure extracting solution light absorption value at 510nm, calculates the extraction rate reached to 2.189% of Folium Mori total flavonoids.
In order to verify the extraction effect of extracting method of the present invention, below several extracting method carried out contrast test:
1) 1g powder of Folium Mori is got in hot reflux, according to the ratio of 1:29g/mL, adds pure water, under 100 ℃ of conditions, extracts 80min, extracting liquid filtering, and standardize solution, in 50mL volumetric flask, is measured light absorption value, calculates total flavones extraction ratio.
2) ultrasonic assisted extraction is got 0.2g powder of Folium Mori, according to the ratio of 1:29g/mL, adds pure water, under 60 ℃ of conditions, extracts 80min, and extracting liquid filtering, standardize solution, in 10mL volumetric flask, are measured light absorption value, calculate total flavones extraction ratio.
3 ﹚ soak and get 0.2g powder of Folium Mori, according to the ratio of 1:29g/mL, add pure water, under room temperature condition, soak 24h, and extracting liquid filtering, standardize solution, in 10mL volumetric flask, are measured light absorption value, calculate total flavones extraction ratio.
As shown in Figure 1, above-mentioned three kinds of method total flavones extraction ratios are compared with total flavones extraction ratio of the present invention, and total flavones extraction ratio of the present invention is apparently higher than other three kinds of methods of the prior art.
Shown in the above and figure is only the preferred embodiment of the present invention.It should be pointed out that for the person of ordinary skill of the art, not departing under the prerequisite of principle of the present invention, can also make some modification and improvement, these also should be considered as belonging to protection scope of the present invention.
Claims (2)
1. from Folium Mori, extract a method for total flavones, comprise the steps:
(1) new fresh mulberry leaf is cleaned, is dried, is pulverized rear 160 orders of crossing and obtain powder of Folium Mori;
(2) in powder of Folium Mori, add a certain amount of pure water, after mix homogeneously, sealing, put into Microwave Extraction Apparatus and carry out microwave-assisted extraction, extract once; Microwave power is 500W, extraction time 2～6min, and extracting temperature is 90～100 ℃;
(3), after having extracted, by extracting liquid filtering, measure total flavones extraction ratio;
In step (2), the ratio of powder of Folium Mori and pure water is at 1:25～1:30g/mL.
2. the method for extracting total flavones from Folium Mori according to claim 1, is characterized in that: after the middle Folium Mori of step (1) are clean, in 50 ℃ of oven dryings, make dried Folium Mori water content below 5%.
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| CN105920415A (en) * | 2016-06-20 | 2016-09-07 | 沈丹虹 | Response surface methodology chequer-shaped indocalamus leaf general flavones ultrasonic extraction method |
| CN107048396A (en) * | 2017-04-14 | 2017-08-18 | 华南农业大学 | A kind of nanometer mulberry leaf powder and preparation method thereof |
| CN109091537B (en) * | 2018-08-22 | 2021-04-20 | 丹阳市田园圣树生态园有限公司 | Supersonic airflow crushing method for mulberry leaves beneficial to improving yield of mulberry leaf extract |
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| CN101712726A (en) * | 2009-10-30 | 2010-05-26 | 浙江工业大学 | Method for preparing high-purity mulberry leaf polysaccharide |
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| CN101712726A (en) * | 2009-10-30 | 2010-05-26 | 浙江工业大学 | Method for preparing high-purity mulberry leaf polysaccharide |
Non-Patent Citations (6)
| Title |
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| "微波法提取桑叶黄酮工艺条件的研究";胡庆国;《淮北煤师院学报》;20100630;第24卷(第2期);32-34 * |
| "微波萃取法提取桑叶和桑白皮的黄酮类成分";陈菁菁等;《中药材》;20061031;第29卷(第10期);1090-1092 * |
| "桑叶黄酮提取分离方法研究";李敏等;《应用化工》;20100630;第39卷(第6期);790-794 * |
| 李敏等."桑叶黄酮提取分离方法研究".《应用化工》.2010,第39卷(第6期),790-794. * |
| 胡庆国."微波法提取桑叶黄酮工艺条件的研究".《淮北煤师院学报》.2010,第24卷(第2期),32-34. * |
| 陈菁菁等."微波萃取法提取桑叶和桑白皮的黄酮类成分".《中药材》.2006,第29卷(第10期),1090-1092. * |
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