CN110073930A - A kind of implantation methods improving radix cynanchi bungei Flavonoid Content - Google Patents
A kind of implantation methods improving radix cynanchi bungei Flavonoid Content Download PDFInfo
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- CN110073930A CN110073930A CN201910398143.5A CN201910398143A CN110073930A CN 110073930 A CN110073930 A CN 110073930A CN 201910398143 A CN201910398143 A CN 201910398143A CN 110073930 A CN110073930 A CN 110073930A
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- radix cynanchi
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- 235000017173 flavonoids Nutrition 0.000 title claims abstract description 28
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- 238000002513 implantation Methods 0.000 title claims abstract description 12
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of implantation methods for improving radix cynanchi bungei Flavonoid Content comprising following steps: step S1, disinfection seed soaking；Step S2, nursery；Step S3, field planting；Step S4 applies organic fertilizer after field planting, after being colonized for 2 ~ March, carry out trickle irrigation to plant root with salt water, and trickle irrigation is primary weekly, and mesohalobic concentration is 3.75 ~ 15g/L.Using technical solution of the present invention, after radix cynanchi bungei field planting, trickle irrigation is carried out using the salt water dialogue tuber of multiflower knotweed root of appropriate concentration, the stimulation coerced using low concentration of salt effectively increases the content of radix cynanchi bungei flavonoids, and radix cynanchi bungei is made to have higher medical value.
Description
Technical field
The present invention relates to radix cynanchi bungei planting technology field more particularly to a kind of plantation sides for improving radix cynanchi bungei Flavonoid Content
Method.
Background technique
Radix cynanchi bungei (scientific name: Cynanchum bungei Decne) belongs to Mo Luoke, and Cynanchum plant is plant cynanchum auriculatum Royle ex Wight
Root tuber, common kind has cynanchum auriculatum Royle, halberd leaf radix cynanchi bungei and three kinds of mountain on the other way round radix cynanchi bungei.Radix cynanchi bungei category Gentianales；Also known as it is white
Ginseng.Ancient books is recorded, and radix cynanchi bungei is used for the late Tang Dynasty, prevailing bright in morning, is used till today.Because of its kidney-supplementing liver-boosting, blackening and generating hair, blood-nourishing benefit
The effect of essence, anti-aging, regimen is considered as by successive dynasties famous expert and prevents old treasure.
The root tuber of radix cynanchi bungei is rich in the substances such as vitamin A, F, G, B, phosphatide, trace selenium, ketone and its hydroxy compounds, these
Ingredient is all effective antioxidant, they can reduce the content of lipid peroxide in the tissue such as serum, liver, brain, cardiopulmonary, is protected
Body is protected from the infringement of free radical, there is splendid immunological regulation and protective effect.Wherein (also known as biological species are yellow for flavonoids
Ketone) it is typical composition in antioxidant, it is the important component in radix cynanchi bungei.The benefit of flavonoids is largely originated from its antioxygen
Change characteristic.Flavonoids can be used as antioxidant and help body counteracting and stabilized radical, inhibit them to healthy cell and tissue
Damage.In this course, flavonoids has the effect of a variety of diseases such as pre- anti-cancer, heart disease, diabetes and tumour.It is known
Flavonoids has anti-inflammatory, antiallergy, ntiviral characteristic.They can reduce arthritis, osteoporosis, allergy, viral disease
Herpes simplex virus caused by disease, influenza virus and adenovirus risk.Another important health benefit of flavonoids be inhibit with
Atherosclerosis and the related platelet aggregation of thrombus.
Therefore, if radix cynanchi bungei Flavonoid Content can be improved, the effective component of radix cynanchi bungei is effectively improved, then radix cynanchi bungei will
With higher medical value,
Summary of the invention
Against the above technical problems, the invention discloses a kind of implantation methods for improving radix cynanchi bungei Flavonoid Content, effectively
Improve the content of radix cynanchi bungei flavonoids.
In this regard, the technical solution adopted by the present invention are as follows:
A kind of implantation methods improving radix cynanchi bungei Flavonoid Content comprising following steps:
Step S1, disinfection seed soaking；
Step S2, nursery；
Step S3, field planting；
Step S4 applies organic fertilizer after field planting, after being colonized for 2~March, carry out trickle irrigation to plant root with salt water, drip weekly
Fill primary, drip-irrigation water amount is 2~3 liters of every cave, and mesohalobic concentration is 3.75~15g/L.
Wherein, salt water is configured with NaCl or sea salt.Further, the concentration of salt water is 3.75~8g/L.
By the study found that utilizing the thorn of low concentration of salt stress using suitable salt water dialogue tuber of multiflower knotweed root progress trickle irrigation
Swash effect, the content of radix cynanchi bungei flavonoids can be effectively improved.But brine strength be higher than 15g/L when, the life of radix cynanchi bungei will be inhibited
It is long, cause its leaf to turn to be yellow, more serious person is even dead.
As a further improvement of the present invention, in step S4, the concentration of the salt water is 7.5g/L.Using the salt of the concentration
Water carries out trickle irrigation, and the effect that the content of radix cynanchi bungei flavonoids improves is most significant.
As a further improvement of the present invention, in step S4, apply organic fertilizer using per acre apply 15~25kg of urea or
Decomposed 450~550kg of muck.
As a further improvement of the present invention, disinfection seed soaking uses following steps in step S1: seed first being immersed time chlorine
5~15 minutes in acid sodium solution, cleaning removal sodium hypochlorite；Then gibberellin-acetone soln 8~16h of seed soaking is used, then is set
In the container that bottom is lined with gauze, water immersion seed is added, centre carries out changing water；After 2~3 days, after seed reveals bud, take out
Nursery.
Further, the mass percent concentration of the sodium hypochlorite is 4%；Seed is immersed in liquor natrii hypochloritis
Afterwards, with tap water to its surface clean 3~4 times, to remove sodium hypochlorite.Further, gibberellin-acetone soln concentration is
20mg/kg, soak seed 12h.Further, changing water frequency is to change a water every 12h.
As a further improvement of the present invention, step S2 nursery uses following steps: by the radix cynanchi bungei kind after disinfection seed soaking
Son is seeded in the seedling medium of seedling rearing room, rearing condition are as follows: 25 DEG C, 3000lux, 12h illumination/12h are dark.
Further, when nursery, by treated, radix cynanchi bungei seed is seeded in the seedling medium of seedling rearing room, seedling medium
For commercially available universal organic substrate Nutrition Soil, pH is 8.0~8.5, and main component is high grade activated carbon, perlite, vermiculite, corruption
The various trace elements such as ripe straw, mushroom leftover bits and pieces, schlempe, chicken manure and nitrogen, phosphorus, potassium, magnesium, manganese.Further, nursery stage
It should not water more, once irrigate, to prevent seedling rotten.
As a further improvement of the present invention, step S3 definite value uses following steps: when seedling it is long to 7~8cm when, transplanting
Radix cynanchi bungei seedling；Radix cynanchi bungei seedling 40~50cm of line-spacing, 20~30cm of spacing in the rows, using 1~2 plant of 1 cave.
As a further improvement of the present invention, step S3 definite value builds grid beam, by rattan after plant length to 20~30cm
In climing traction to rack.
Compared with prior art, the invention has the benefit that
First, the salt water dialogue Polygonum Multiflorum root of appropriate concentration is utilized after radix cynanchi bungei field planting using technical solution of the present invention
Portion carries out trickle irrigation, and the stimulation coerced using low concentration of salt effectively increases the content of radix cynanchi bungei flavonoids.
Second, it using technical solution of the present invention, before sowing, carries out disinfection to seed, then uses gibberellin-acetone soln
Seed soaking, is finally placed in water germination, had not only carried out sterilizing to seed, but also have stimulated seed cell enzyme activity using gibberellin
Property, effectively increase germination and emergence rate.Nursery stage carries out breeding, suitable temperature and illumination condition using Standard culture conditions
It ensure that the stability of breeding environment, so that radix cynanchi bungei seedling has good physiological status, be conducive to transplant planting.To hoary head
After black seedling slightly grows up, in transplanting to ground, and takes rack and stretched for radix cynanchi bungei tendril, grid beam structure ensure that radix cynanchi bungei can fill
Point ground receives photosynthesis, and three-dimensional is conducive to the circulation of the gas between radix cynanchi bungei plant, and bottom will not occur for when hot weather
Portion's putrefactive phenomenon.
Specific embodiment
Preferably embodiment of the invention is described in further detail below.
The embodiment of the present invention is with cynanchum auriculatum Royle (i.e. radix cynanchi bungei) for experimental subjects, and seed is by Littoral Jiangsu Province agricultural
Science Institute provides, and specific experiment carries out in the plant room of our company.
The plantation of radix cynanchi bungei is carried out using following steps:
(1) disinfection seed soaking: seed is immersed in 4% liquor natrii hypochloritis 10 minutes, then with tap water to its surface
Cleaning 3~4 times, to remove sodium hypochlorite.It is soaked seed 12h with the gibberellin of 20mg/kg-acetone soln, then places it in bottom pad
Have in the container of gauze, appropriate water immersion seed is added, a water was changed every 12 hours.After 2~3 days, after seed reveals bud, take
Nursery out.
(2) nursery: by treated, radix cynanchi bungei seed is seeded in the seedling medium of seedling rearing room, rearing condition are as follows: and 25 DEG C,
3000lux, 12h illumination/12h are dark.
(3) be colonized: when seedling it is long to 7~8cm when, transplant radix cynanchi bungei seedling.Radix cynanchi bungei seedling 40~50cm of line-spacing, spacing in the rows
20~30cm, using 1~2 plant of 1 cave.After plant length to 20~30cm, grid beam is built with thin bamboo or other materials, by rattan
In climing traction to rack.
(4) it applies fertilizer: applying organic fertilizer after field planting, apply urea 20kg or decomposed muck 500kg or so per acre.Field planting 2 months
Afterwards, trickle irrigation is carried out to plant root with salt water, trickle irrigation is primary weekly, and drip-irrigation water amount is 2~3 liters of every cave.
Salt water is configured with the sea salt dried, is provided with 5 groups of experiments altogether, brine strength is respectively 0,3.75,7.5,15,30g/
L, every group of three samples.After salt water trickle irrigation 2 months, there is the phenomenon that leaf jaundice, 30g/L in the radix cynanchi bungei plant of 15g/L
Radix cynanchi bungei plant it is dead.The root tuber for then taking out four groups of cynanchum auriculatum Royles of 0,3.75,7.5,15g/L, after cleaning up
Its Flavonoid Content is determined, measuring method is the plant flavonoids detection reagent that Bioengineering Research Institute is built up in Nanjing
Box, specific data are as shown in table 1.
The cynanchum auriculatum Royle Flavonoid Content of the different brine strength trickle irrigations of table 1
By the data comparison of upper table as it can be seen that technical solution of the present invention is used, so that the class of cynanchum auriculatum Royle root tuber is yellow
Ketone content has biggish promotion, substantially increases the medical value of radix cynanchi bungei.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
Specific implementation of the invention is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to of the invention
Protection scope.
Claims (7)
1. it is a kind of improve radix cynanchi bungei Flavonoid Content implantation methods, which is characterized in that itself the following steps are included:
Step S1, disinfection seed soaking；
Step S2, nursery；
Step S3, field planting；
Step S4 applies organic fertilizer after field planting, after being colonized for 2 ~ March, carry out trickle irrigation to plant root with salt water, weekly trickle irrigation one
Secondary, drip-irrigation water amount is 2 ~ 3 liters of every cave, and mesohalobic concentration is 3.75 ~ 15g/L.
2. the implantation methods according to claim 1 for improving radix cynanchi bungei Flavonoid Content, which is characterized in that in step S4,
The concentration of the salt water is 7.5g/L.
3. the implantation methods according to claim 1 for improving radix cynanchi bungei Flavonoid Content, which is characterized in that in step S4,
Apply organic fertilizer using application 15 ~ 25kg of urea or decomposed 450 ~ 550kg of muck per acre.
4. the implantation methods according to claim 1 for improving radix cynanchi bungei Flavonoid Content, which is characterized in that disappear in step S1
Poison seed soaking uses following steps: first immersing seed in liquor natrii hypochloritis 5 ~ 15 minutes, cleaning removal sodium hypochlorite；Then it uses
Gibberellin-acetone soln is soaked seed 8 ~ 16h, then is placed it in bottom and be lined in the container of gauze, is added water immersion seed, it is intermediate into
Row changes water；After 2 ~ 3 days, after seed reveals bud, nursery is taken out.
5. the implantation methods according to claim 1 for improving radix cynanchi bungei Flavonoid Content, which is characterized in that step S2 nursery
Using following steps: the radix cynanchi bungei seed after disinfection seed soaking being seeded in the seedling medium of seedling rearing room, rearing condition are as follows: 25
DEG C, 3000 lux, 12 h illumination/12 h are dark.
6. the implantation methods according to claim 1 for improving radix cynanchi bungei Flavonoid Content, which is characterized in that step S3 definite value
Using following steps: when seedling it is long to 7 ~ 8cm when, transplant radix cynanchi bungei seedling；Radix cynanchi bungei seedling 40 ~ 50cm of line-spacing, spacing in the rows 20 ~
30cm, using 1 ~ 2 plant of 1 cave.
7. the implantation methods according to claim 6 for improving radix cynanchi bungei Flavonoid Content, which is characterized in that step S3 definite value
After plant length to 20 ~ 30cm, grid beam is built, tendril is drawn to rack.
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| CN115226519A (en) * | 2022-08-31 | 2022-10-25 | 青岛农业大学 | Method for promoting increase of total flavone content in scutellaria baicalensis |
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