CN110073901A - A kind of edible fungus culturing sterilizing methods - Google Patents

A kind of edible fungus culturing sterilizing methods Download PDF

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Publication number
CN110073901A
CN110073901A CN201910420048.0A CN201910420048A CN110073901A CN 110073901 A CN110073901 A CN 110073901A CN 201910420048 A CN201910420048 A CN 201910420048A CN 110073901 A CN110073901 A CN 110073901A
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China
Prior art keywords
sterilization
ultraviolet
edible fungus
culture medium
sterilizing
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CN201910420048.0A
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Chinese (zh)
Inventor
陈远德
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Chizhou Defeng Industry LLC
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Chizhou Defeng Industry LLC
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Priority to CN201910420048.0A priority Critical patent/CN110073901A/en
Publication of CN110073901A publication Critical patent/CN110073901A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/30Accessories for use before inoculation of spawn, e.g. sterilisers
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/04Heat
    • A61L2/06Hot gas
    • A61L2/07Steam
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/08Radiation
    • A61L2/10Ultraviolet radiation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

A kind of edible fungus culturing sterilizing methods, comprising the following steps: S1 preheating;Culture medium in sterilization bin is preheated, preheating time t1;S2 steam sterilizing: S3 is dry: it is dry to carry out forced ventilation to sterilization bin;S4 ultraviolet-sterilization;Ultraviolet light is irradiated to the culture medium after drying, the ultraviolet-sterilization time is t3.Culture medium using above method sterilizing front and back is compared, discovery sterilization rate is high.

Description

A kind of edible fungus culturing sterilizing methods
Technical field
The present invention relates to technical field of edible fungi cultivation more particularly to a kind of edible fungus culturing sterilizing methods.
Background technique
Edible mushroom refers to that fructification is very large, gill fungus bacterium (macro fungi) that can be edible, is commonly referred to as mushroom.Chinese known food Have more than 350 kinds with bacterium, wherein belong to Basidiomycotina, common are morely: mushroom, straw mushroom, mushroom, agaric, tremella, hedgehog hydnum, dictyophora phalloidea, Trichotoma matsutake (matsutake), dried mushroom, russule, ganoderma lucidum, cordyceps sinensis, truffle, lark and bolete etc.;Minority belongs to Ascomycotina, wherein Have: hickory chick, saddle fungus, ferfas etc..
Edible mushroom is not only delicious, but also full of nutrition, is often referred to as healthy food by people.The protein content of mushroom class is general For fresh mushroom 1.5-6%, dry mushroom 15-35%, it is higher than general vegetables, and its amino acid composition is more comprehensive, most mushroom class contains Have eight kinds of amino acid necessary to human body, wherein mushroom, straw mushroom, lysine content is abundant in needle mushroom.And there are many mushroom class contains Vitamin and a variety of mineral elements with physiological activity, such as VB1, VB12, Vc, Vk, VD and phosphorus, sodium, potassium, calcium, iron and many Microelement can supplement the deficiency in other food.Edible mushroom also has good medical value simultaneously.Contain in edible mushroom Bioactive substance is such as: macromolecule polysaccharide, β-glucose and RNA complex, natural organic??, nucleic acid degradation product, cAMP and three Terpenoid etc. has important utility value to maintenance human health, often produces preparation health care using it as raw material by businessman now Food, health drink, wine and drug are largely used to medicinal and investment health-product market.
The mode of edible fungus culturing is varied.By compost point, there is segment wood cultivated and substituting stuff cultivation;By planting type Point, there are ridge cultivation, bed cultivation and case to plant;By culture place point, there are indoor growing, outdoor planting etc..It is segment wood cultivated fewer and fewer, reason It is because destroying forest tree resource.The substituting stuff cultivation of edible mushroom includes cultivation in raw material, clinker cultivation and fermentation material cultivation.Cultivation in raw material Refer to the method that edible fungus culturing is directly used in after adding water to mix thoroughly compost according to formula;Clinker cultivation refers to will according to formula Compost adds water to mix thoroughly, packs, sterilize after inoculate the method for culturing edible fungus;Fermentation material cultivation, which refers to, adds water to mix compost It is fermented after even to kill harmful miscellaneous bacteria and worm's ovum, and keeps compost decomposed, the method for being subsequently used for edible fungus culturing.For giving birth to Expect that the materials such as the cotton seed hulls of cultivation must be fresh, does not agglomerate, do not go mouldy, temperature is at 15 DEG C or less.It is planted for clinker and fermentation material The material of training need not be fresh, high temperature, low temperature season.Oyster mushroom can use cultivation in raw material;Agaricus bisporus, coprinus comatus, Brazilian mushroom, Gold oyster mushroom etc. can be cultivated with fermentation material;Ganoderma lucidum, mushroom, needle mushroom etc. are cultivated with clinker.Agaricus bisporus, Brazilian mushroom etc. are multi-purpose Bed is planted, other edible mushrooms are mostly used bag and plant.
Cultivation matrix is the place for the various nutriments that edible mushroom depends on for existence.Using kind and growing environment condition In identical situation, formula difference different using matrix, the processing mode of matrix are different, the yield of edible mushroom, quality, flavor Deng all difference.
Existing medium sterilization effect is not in place, for example, the independent sterilization rate of general ultraviolet light is 93%-95%, goes out Bacterium rate is not high, will affect subsequent cultivating rate.
Summary of the invention
The object of the present invention is to provide a kind of edible fungus culturing sterilizing methods, and sterilization rate is high, and sterilization effect is good.
To realize the above-mentioned technical purpose, the technical solution of the embodiment of the present invention is specific as follows.
A kind of edible fungus culturing sterilizing methods, comprising the following steps:
S1 preheating;Culture medium in sterilization bin is preheated, preheating time t1;
S2 steam sterilizing: being passed through sterilization bin for superheated steam and sterilize, steam sterilizing time t2, in the sterilization bin Pressure be greater than 1 atmospheric pressure;
S3 is dry: it is dry to carry out forced ventilation to sterilization bin;
S4 ultraviolet-sterilization;Ultraviolet light is irradiated to the culture medium after drying, the ultraviolet-sterilization time is t3.
Further, the preheating time t1 in the step S1 in sterilization bin is 1-3 hours, and preheating temperature is 70 degree -100 Degree.
Further, the pressure in the step S2 in sterilization bin be 1.1-1.5 atmospheric pressure, vapor (steam) temperature 150-170 degree, Sterilization time t2 is 35-60 seconds.
Further, in the step S3, the forced ventilation time is 0.5-1 hours.
Further, it is 1-3 minutes that the ultraviolet-sterilization time, which is t3,.
Further, the ultraviolet-sterilization uses LED ultraviolet sterilization lamp, and the LED ultraviolet sterilization lamp setting is being gone out In bacterium storehouse.
Further, ultraviolet sterilization is carried out simultaneously in the step S2.
Compared with prior art, this have the advantage that:
A kind of edible fungus culturing sterilizing methods, comprising the following steps:
S1 preheating;Culture medium in sterilization bin is preheated, preheating time t1;By preheating so that subsequent overheat is steamed Vapour the case where superheated steam condensation will not occur after entering sterilization bin again, this can condense enterprising in culture medium to avoid superheated steam Caused by culture medium make moist the problem of.
S2 steam sterilizing: being passed through sterilization bin for superheated steam and sterilize, steam sterilizing time t2, in the sterilization bin Pressure be greater than 1 atmospheric pressure;In the environment of high pressure, the miscellaneous bacteria in culture medium can be preferably killed, avoids influencing subsequent Edible fungi inoculation.
S3 is dry: it is dry to carry out forced ventilation to sterilization bin;After steam sterilizing, it will lead to steam and penetrate into culture medium, shadow Ring culture medium quality, it is therefore desirable to be dried, use forced ventilation drying that can reduce the infection of other miscellaneous bacterias with rapid draing.
S4 ultraviolet-sterilization;Ultraviolet light is irradiated to the culture medium after drying, the ultraviolet-sterilization time is t3.Forced ventilation is dried Cheng Zhong may introduce miscellaneous bacteria, therefore carry out re-pasteurization using ultraviolet light, improve bactericidal effect.
Culture medium using above method sterilizing front and back is compared, discovery sterilization rate is high.
Specific embodiment
Technical solution of the present invention is described in detail below, it is clear that described embodiment is a part of the invention Embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making wound Every other embodiment obtained under the premise of the property made labour, shall fall within the protection scope of the present invention.
Embodiment 1
A kind of edible fungus culturing sterilizing methods, comprising the following steps:
S1 preheating;Culture medium in sterilization bin is preheated, preheating time t1;Further, pre- in sterilization bin Hot time t1 is 1 hour, and preheating temperature is 70 degree.Will not occur again after preheating so that subsequent superheated steam enters sterilization bin Superheated steam condense the case where, this can to avoid superheated steam condense caused by culture medium is enterprising culture medium make moist ask Topic.
S2 steam sterilizing: being passed through sterilization bin for superheated steam and sterilize, steam sterilizing time t2, in the sterilization bin Pressure be greater than 1 atmospheric pressure;Further, the pressure in the step S2 in sterilization bin is 1.1 atmospheric pressure, vapor (steam) temperature 150 degree, sterilization time t2 is 35 seconds.In the environment of high pressure, the miscellaneous bacteria in culture medium can be preferably killed, after avoiding influence Continuous edible fungi inoculation.
S3 is dry: it is dry to carry out forced ventilation to sterilization bin;In the step S3, the forced ventilation time is 0.5 small When.After steam sterilizing, it will lead to steam and penetrate into culture medium, influence culture medium quality, it is therefore desirable to be dried, using pressure Aeration-drying can reduce the infection of other miscellaneous bacterias with rapid draing.
S4 ultraviolet-sterilization;Ultraviolet light is irradiated to the culture medium after drying, the ultraviolet-sterilization time is t3.When the ultraviolet-sterilization Between be t3 be 1 minute.The ultraviolet-sterilization uses LED ultraviolet sterilization lamp, and the LED ultraviolet sterilization lamp is arranged in sterilization bin In.In forced ventilation drying process, miscellaneous bacteria may be introduced, therefore re-pasteurization is carried out using ultraviolet light, improve bactericidal effect.
Culture medium using above method sterilizing front and back is compared, discovery sterilization rate reaches 98.7%.
Preferably, ultraviolet sterilization is carried out simultaneously in abovementioned steps S2, steam sterilizing and ultraviolet sterilization carry out simultaneously It more efficiently can thoroughly sterilize.
Embodiment 2
A kind of edible fungus culturing sterilizing methods, comprising the following steps:
S1 preheating;Culture medium in sterilization bin is preheated, preheating time t1;Further, pre- in sterilization bin Hot time t1 is 1-3 hours, and preheating temperature is 70 degree of -100 degree.After preheating so that subsequent superheated steam enters sterilization bin not Can occur the case where superheated steam condensation again, this can to avoid superheated steam condense caused by culture medium is enterprising culture medium by The problem of tide.
S2 steam sterilizing: being passed through sterilization bin for superheated steam and sterilize, steam sterilizing time t2, in the sterilization bin Pressure be greater than 1 atmospheric pressure;Further, the pressure in the step S2 in sterilization bin is 1.5 atmospheric pressure, vapor (steam) temperature 170 degree, sterilization time t2 is 60 seconds.In the environment of high pressure, the miscellaneous bacteria in culture medium can be preferably killed, after avoiding influence Continuous edible fungi inoculation.
S3 is dry: it is dry to carry out forced ventilation to sterilization bin;In the step S3, the forced ventilation time is 1 hour. After steam sterilizing, it will lead to steam and penetrate into culture medium, influence culture medium quality, it is therefore desirable to it is dried, it is logical using forcing Air-dry it is dry can with rapid draing, reduce other miscellaneous bacterias infection.
S4 ultraviolet-sterilization;Ultraviolet light is irradiated to the culture medium after drying, the ultraviolet-sterilization time is t3.When the ultraviolet-sterilization Between be t3 be 3 minutes.The ultraviolet-sterilization uses LED ultraviolet sterilization lamp, and the LED ultraviolet sterilization lamp is arranged in sterilization bin In.In forced ventilation drying process, miscellaneous bacteria may be introduced, therefore re-pasteurization is carried out using ultraviolet light, improve bactericidal effect.
Culture medium using above method sterilizing front and back is compared, discovery sterilization rate reaches 99.5%.
Embodiment 3
A kind of edible fungus culturing sterilizing methods, comprising the following steps:
S1 preheating;Culture medium in sterilization bin is preheated, preheating time t1;Further, pre- in sterilization bin Hot time t1 is 2 hours, and preheating temperature is 80 degree.Will not occur again after preheating so that subsequent superheated steam enters sterilization bin Superheated steam condense the case where, this can to avoid superheated steam condense caused by culture medium is enterprising culture medium make moist ask Topic.
S2 steam sterilizing: being passed through sterilization bin for superheated steam and sterilize, steam sterilizing time t2, in the sterilization bin Pressure be greater than 1 atmospheric pressure;Further, the pressure in the step S2 in sterilization bin is 1.3 atmospheric pressure, vapor (steam) temperature 160 degree, sterilization time t2 is 45 seconds.In the environment of high pressure, the miscellaneous bacteria in culture medium can be preferably killed, after avoiding influence Continuous edible fungi inoculation.
S3 is dry: it is dry to carry out forced ventilation to sterilization bin;In the step S3, the forced ventilation time is 0.8 small When.After steam sterilizing, it will lead to steam and penetrate into culture medium, influence culture medium quality, it is therefore desirable to be dried, using pressure Aeration-drying can reduce the infection of other miscellaneous bacterias with rapid draing.
S4 ultraviolet-sterilization;Ultraviolet light is irradiated to the culture medium after drying, the ultraviolet-sterilization time is t3.When the ultraviolet-sterilization Between be t3 be 2 minutes.The ultraviolet-sterilization uses LED ultraviolet sterilization lamp, and the LED ultraviolet sterilization lamp is arranged in sterilization bin In.In forced ventilation drying process, miscellaneous bacteria may be introduced, therefore re-pasteurization is carried out using ultraviolet light, improve bactericidal effect.
Culture medium using above method sterilizing front and back is compared, discovery sterilization rate reaches 99.2%.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements It both falls in the scope of protection of present invention.The claimed scope of the invention is by appended claims and its equivalent circle It is fixed.

Claims (7)

1. a kind of edible fungus culturing sterilizing methods, which comprises the following steps:
S1 preheating;Culture medium in sterilization bin is preheated, preheating time t1;
S2 steam sterilizing: being passed through sterilization bin for superheated steam and sterilize, steam sterilizing time t2, the pressure in the sterilization bin Power is greater than 1 atmospheric pressure;
S3 is dry: it is dry to carry out forced ventilation to sterilization bin;
S4 ultraviolet-sterilization;Ultraviolet light is irradiated to the culture medium after drying, the ultraviolet-sterilization time is t3.
2. edible fungus culturing sterilizing methods as described in claim 1, which is characterized in that pre- in sterilization bin in the step S1 Hot time t1 is 1-3 hours, and preheating temperature is 70 degree of -100 degree.
3. edible fungus culturing sterilizing methods as described in claim 1, which is characterized in that the pressure in the step S2 in sterilization bin Power is 1.1-1.5 atmospheric pressure, and vapor (steam) temperature 150-170 degree, sterilization time t2 is 35-60 seconds.
4. edible fungus culturing sterilizing methods as described in claim 1, which is characterized in that described to force to lead in the step S3 The wind time is 0.5-1 hours.
5. such as edible fungus culturing sterilizing methods of any of claims 1-4, which is characterized in that when the ultraviolet-sterilization Between be t3 be 1-3 minutes.
6. edible fungus culturing sterilizing methods as claimed in claim 5, which is characterized in that the ultraviolet-sterilization is ultraviolet using LED Line bactericidal lamp, the LED ultraviolet sterilization lamp are arranged in sterilization bin.
7. edible fungus culturing sterilizing methods as claimed in claim 5, which is characterized in that carry out purple simultaneously in the step S2 Outside line sterilization.
CN201910420048.0A 2019-05-20 2019-05-20 A kind of edible fungus culturing sterilizing methods Pending CN110073901A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1069634A (en) * 1963-06-19 1967-05-24 Amsco Internat S A Improvements in or relating to the sterilization of goods
CN1482870A (en) * 2000-12-22 2004-03-17 ���ṫ˾ Pasteurizing or sterilizing
CN101732740A (en) * 2010-01-07 2010-06-16 兰州奇正粉体装备技术有限公司 Steam sterilizing method
CN103004472A (en) * 2012-12-29 2013-04-03 沈阳恒生农业发展有限公司 Sterilization process method for bottle-planted needle mushroom factory production
CN104303821A (en) * 2014-09-15 2015-01-28 管兴 Method for cultivating pleurotus eryngii industrially
CN107050476A (en) * 2017-05-03 2017-08-18 山东东阿东盛阿胶产品科技开发有限公司 A kind of donkey-hide gelatin block sterilization process

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1069634A (en) * 1963-06-19 1967-05-24 Amsco Internat S A Improvements in or relating to the sterilization of goods
CN1482870A (en) * 2000-12-22 2004-03-17 ���ṫ˾ Pasteurizing or sterilizing
CN101732740A (en) * 2010-01-07 2010-06-16 兰州奇正粉体装备技术有限公司 Steam sterilizing method
CN103004472A (en) * 2012-12-29 2013-04-03 沈阳恒生农业发展有限公司 Sterilization process method for bottle-planted needle mushroom factory production
CN104303821A (en) * 2014-09-15 2015-01-28 管兴 Method for cultivating pleurotus eryngii industrially
CN107050476A (en) * 2017-05-03 2017-08-18 山东东阿东盛阿胶产品科技开发有限公司 A kind of donkey-hide gelatin block sterilization process

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