CN110050680A - A kind of Indian beech seedling quick efficient breeding method - Google Patents
A kind of Indian beech seedling quick efficient breeding method Download PDFInfo
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- CN110050680A CN110050680A CN201910361093.3A CN201910361093A CN110050680A CN 110050680 A CN110050680 A CN 110050680A CN 201910361093 A CN201910361093 A CN 201910361093A CN 110050680 A CN110050680 A CN 110050680A
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- indian beech
- seed
- indian
- seedling
- beech
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of Indian beech seedling quick efficient breeding method, comprising the following specific steps collecting mature Indian beech fruit, the Indian beech fruit is put in Indoor Natural to air-dry, then the exocarp of the Indian beech fruit is removed, obtain Indian beech seed, choose gibberellin aqueous solution, then the Indian beech seed is placed in the gibberellin aqueous solution and is impregnated, prepare a sand bed, the Indian beech seed after immersion is seeded in the sand bed, the final Indian beech seed is sprouted, then grow up to Indian beech seedling, rapidly and efficiently nursery is carried out by material of Indian beech seed, realize that Indian beech seed starts sprout time and shifts to an earlier date 20d or more, seed germination rate reaches 95% or more, seedling transplanting survival rate reaches 90%, and produce the high-quality Indian beech seedling of neat and consistent, it is quickly high to establish Indian beech seedling Breeding of method is imitated, provides effective solution with resources development and utilization to meet the urgent need of Indian beech seedling and restoring protection.
Description
Technical field
The present invention relates to plant cover cultivation methods fields, more particularly to a kind of Indian beech seedling quick efficient breeding method.
Background technique
Indian beech (Pongamia pinnata) belongs to pulse family (Leguminosae) Pongamia (Pongamia).Arbor, it is high
8-15 meters.Spray is usually hairless, sometimes slightly by micro- pubescence, the dense canescence small skin hole of old branch.Pod is 4-5 centimetres long, wide 1.5-
2.5 centimetres, surface has not very apparent small wart convex, and there is the short beak of microbend on top, does not crack, side along suture without protuberance or
Wing has 1, seed；Seed kidney shape.The month at florescence 5-6, the fruiting period 8-10 month.Produce Fujian, Guangdong (southeast coastal area), Hainan.
It is born in the place that small stream side, the side of a pond and seashore tide energy reach.India, Sri Lanka, Malaysia, Australia, Pohle Ni Xi
Asia is also distributed.The Indian beech grain of wood is fine and close beautiful, can make various utensils；Seed oil can make fuel；Complete stool is used as medicine, and can be made
Emetic and insecticide, oil treatment scabies leprosy, running sore and the rheumatism squeezed out with seed.In India, seed and seed oil are used to
Leucoplakia, leprosy, lumbar rheumatism pain, rheumarthritis are treated, leaf is for treating the illnesss such as hemorrhoid, tumour, diminishing inflammation of wound.
Indian beech tree crown is open, and branches and leaves are dense, and tree performance is graceful, and the performances such as wind resistance, drought resisting, anti-air pollution and resistance to water-wet are strong, to soil
Salinity has certain tolerance, and the coastal bank Plantation Growth that can be suitble in salinity, is excellent Seaboard afforestation tree species, on edge
Haiti Qu Kezuo embankment is protected a forest and shade tree；
The mankind to conservation of nature consciousness not enough, habitat destroyed etc. due to make the natural money of this kind of Indian beech plant
Source is extremely limited.The development and utilization of mangrove associates Indian beech is the urgent need in production, since production needs to educate with existing
Seedling technical restriction, Indian beech seed seedling-raising germination rate is low at present, and sprout time is long, and existing Indian beech is using seed seedling-raising
Method is at high cost, reproductive efficiency is low, therefore is badly in need of a kind of efficient, scale Indian beech mating system.
Summary of the invention
To solve the above problems, the present invention adopts the following technical scheme: a kind of Indian beech seedling quick efficient breeding method,
Comprising the following specific steps
S1 collects mature Indian beech fruit；
The Indian beech fruit is put in Indoor Natural and air-dried by S2, is then removed the exocarp of the Indian beech fruit, is obtained
To Indian beech seed；
S3 chooses gibberellin aqueous solution, and then the Indian beech seed is placed in the gibberellin aqueous solution and is impregnated；
S4 prepares a sand bed, and the Indian beech seed after immersion is seeded in the sand bed, the final Indian beech
Seed is sprouted, and Indian beech seedling is then grown up to.
Further, in the step S3, the concentration of the gibberellin aqueous solution is preferably 0~200mg/L.
Further, in the step S3,0~48h of soaking time of the Indian beech seed.
Further, in the step S3, the soaking time of the Indian beech seed is 6~48h.
Further, in the step S3, the soaking time of the Indian beech seed is 24~48h.
Further, in the step S4, the relative humidity of the sand bed is controlled 60%~80%.
Further, in the step S4, the Germination Temperature of the Indian beech seed is 23 DEG C~28 DEG C.
Further, in the step S2, the mode for removing the exocarp of the Indian beech fruit is manual removal.
Further, in the step S2, the mode of the exocarp of the Indian beech fruit is removed by the Indian beech
The exocarp of fruit manual removal after clear water impregnates 20min.
Further, in the step S4, the time that the Indian beech seed is sprouted is 8~12d.
The invention has the benefit that (1) carries out rapidly and efficiently nursery by material of Indian beech seed, Indian beech kind is realized
Son starts sprout time and shifts to an earlier date 20d or more, and seed germination rate reaches 95% or more, and seedling transplanting survival rate reaches 90%, and raw
Produce the high-quality Indian beech seedling of neat and consistent.
(2) Indian beech seedling rapidly and efficiently breeding of method is established, to meet the urgent need of Indian beech seedling and restoring to protect
Shield provides effective solution with resources development and utilization.
Detailed description of the invention
Fig. 1 is flow chart of the present invention.
Specific embodiment
Below with reference to the attached drawing of the embodiment of the present invention, technical solution of the present invention is described further, the present invention is not
It is only limitted to following specific embodiments.It should be noted that in the absence of conflict, embodiment and embodiment in the present invention
In feature can be combined with each other.
Embodiment 1
As shown in Figure 1, a kind of Indian beech seedling quick efficient breeding method, comprising the following specific steps
S1 collects mature Indian beech fruit.
Specifically, it by collecting mature Indian beech fruit, is then put in spare in laboratory.
The Indian beech fruit is put in Indoor Natural and air-dried by S2, is then removed the exocarp of the Indian beech fruit, is obtained
To Indian beech seed.
Specifically, spare Indian beech fruit in laboratory will be put in and carry out natural air drying, then by Indian beech fruit with
Machine is uniformly divided into test group 1, test group 2, test group 3, test group 4, test group 5, test group 6 and test group 7, and 60 every part.
The test group 1 is the whole fruit for not removing exocarp, test group 2, test group 3, test group 4, test group 5,6 and of test group
Test group 7 is the Indian beech seed for removing exocarp.
S3 chooses gibberellin aqueous solution, and then the Indian beech seed is placed in the gibberellin aqueous solution and is impregnated.
Specifically, to be contrasted, test group 1 and test group 2 do not use gibberellin aqueous solution to be soaked in the present embodiment
Bubble, by the seed of test group 3~7 be respectively placed in clear water impregnate 0h, 6h, 12h, for 24 hours, 48h.
S4 prepares a sand bed, and the Indian beech seed after immersion is seeded in the sand bed, the final Indian beech
Seed is sprouted, and Indian beech seedling is then grown up to.
Specifically, the Indian beech seed of the whole fruit of test group 1 and test group 2~7 is seeded in wet sand respectively
Seed Germination Test is carried out in bed.Germination Temperature is 23 DEG C~28 DEG C.Sprinkling proper amount of clear water daily guarantees that sand bed humid control exists
60%~80%, continue 60d and observes and records seed sprouting situation.
Seed Germination Test is carried out using the above method, test result is shown in Table 1:
1. Indian beech seed of table sows the sprouting situation after 60d
As shown in Table 1:
(1) the whole fruit seed of test group 1 starts to sprout after sowing 28d, germination rate 35.6%；Test group 2
Removal exocarp seed starts to sprout after sowing 16d, germination rate 68.2%.The seed sprout time of test group 2 is than test
Group 1 is advanced by 12d and seed germination rate is significantly higher than test group 1.By test group 1 and test group 2 it is found that outside removal Indian beech
Pericarp has positive facilitation to seed sprout time and germination rate, but under the processing sprout time of seed it is longer and
Germination rate is lower, does not reach seedling breeding requirement rapidly and efficiently yet.
(2) the seed germination rate ratio into sand bed is directly sowed by impregnating (6 without the Indian beech seed of immersion treatment
~48h) seed germination rate want low.The seed of test group 4 starts to sprout in after planting 16d, and the seed of test group 5~7 is being broadcast
Start to sprout within 14 days after kind, is advanced by 2d than test group 3.The seed germination rate of test group 4~7 is respectively 72.8%, 79.6%,
83.6%, 83.5%.Therefore Indian beech seed can be improved seed germination rate by immersion treatment, and when soaking time reaches
Seed can be shortened when 12h or more and start sprout time.By immersion treatment can be improved seed germination rate reach 83.6% with
On, although immersion treatment has reached higher seed germination rate, seed starts sprout time and still takes a long time.
That is, removal Indian beech exocarp has positive facilitation to seed sprout time and germination rate, and
It can be improved seed germination rate by immersion treatment and reach 83.6% or more.
Embodiment 2
For determining that various concentration gibberellin aqueous solution soaking handles the influence sprouted to seed.
A kind of Indian beech seedling quick efficient breeding method, comprising the following specific steps
S1 collects mature Indian beech fruit.
Specifically, it by collecting mature Indian beech fruit, is then put in spare in laboratory.
The Indian beech fruit is put in Indoor Natural and air-dried by S2, is then removed the exocarp of the Indian beech fruit, is obtained
To Indian beech seed.
Specifically, by Indian beech fruit removal exocarp, simultaneously stochastic averagina is divided into 4 parts, 60 every part, is respectively labeled as trying
Test group 8~11.
S3 chooses gibberellin aqueous solution, and then the Indian beech seed is placed in the gibberellin aqueous solution and is impregnated.
Specifically, by test group 5~7 it is found that Indian beech seed starts to sprout required time most when soaking time is for 24 hours
It is short, and germination rate highest, therefore, by the seed of test group 8~11 be soaked in respectively concentration be 0,50mg/L, 100mg/L and
It is impregnated for 24 hours in the gibberellin aqueous solution of 200mg/L.
S4 prepares a sand bed, and the Indian beech seed after immersion is seeded in the sand bed, the final Indian beech
Seed is sprouted, and Indian beech seedling is then grown up to.
By treated, seed is seeded in respectively in wet sand bed carries out Seed Germination Test.Germination Temperature be 23 DEG C~
28℃.Sprinkling proper amount of clear water daily guarantees that sand bed humid control 60%~80%, continues 60d and observes and records seed sprouting
Situation.
Seed Germination Test is carried out using the above method, test result is shown in Table 2:
2. various concentration Gibberellins solution of table impregnates the influence for handling sprout to Indian beech seed for 24 hours
As shown in Table 2, start to sprout in 8d by the Indian beech seed that 100~200mg/L Gibberellins solution is impregnated, try
The seed for testing group 8 and test group 9 sprouts required time respectively 14d and 12d, can make Indian beech by Gibberellins solution immersion
Seed shifts to an earlier date 6d sprouting.The seed germination rate highest of test group 10 reaches 95.5%, therefore the gibberellin of 100mg/L impregnates for 24 hours
The optimal immersion treatment sprouted for Indian beech seed.
That is, when using the gibberellin aqueous solution of 100mg/L to impregnate Indian beech seed, and soaking time is
When for 24 hours, rapidly and efficiently nursery can be carried out, realize that Indian beech seed starts sprout time and shifts to an earlier date 20d or more, seed germination rate reaches
95% or more.
In above-described embodiment, Indian beech seedling replanting is tested, the specific method is as follows:
The Indian beech seedling for the robust growth that the processing of test group 10 obtains is transferred to hardening in ventilated cool canopy
10d is mixed later by the mixed-matrix of the seedling replanting after domestication to perlite, peat soil and the sand disinfected in advance
Composition and division in a proportion example is 2:2:1.The mixed-matrix using it is preceding first use 0.1% disinfecting solution of potassium permanganate, when the shifting of Indian beech seedling
It is sprinkled profoundly water immediately after cultivation.
Indian beech seedling replanting is carried out using the above method, as a result as follows:
New tender shoots is grown after Indian beech seedling replanting 17d, survival rate is 90% after transplanting 30d.
That is, Indian beech seedling rapidly and efficiently breeding of method can be established by the method, meet Indian beech well
The urgent need of seedling and the demand for restoring protection with resources development and utilization.
Specifically, in embodiment 1 and embodiment 2, in the step S2, the exocarp of the Indian beech fruit is removed
Mode be manual removal.Further, in embodiment 1 and embodiment 2, in the step S2, the Indian beech is removed
The mode of the exocarp of fruit manual removal after clear water impregnates 20min by the exocarp of the Indian beech fruit.That is,
Two kinds of removing modes can well remove the exocarp of Indian beech fruit.
In conclusion it is restricted embodiment of the invention, all those skilled in the art that above embodiment, which is not,
The modification carried out on the basis of substantive content of the invention or equivalent deformation, in technology scope of the invention.
Claims (10)
1. a kind of Indian beech seedling quick efficient breeding method, which is characterized in that comprising the following specific steps
S1 collects mature Indian beech fruit；
The Indian beech fruit is put in Indoor Natural and air-dried by S2, is then removed the exocarp of the Indian beech fruit, is obtained water
Radical Injury in Wampee Seeds；
S3 chooses gibberellin aqueous solution, and then the Indian beech seed is placed in the gibberellin aqueous solution and is impregnated；
S4 prepares a sand bed, and the Indian beech seed after immersion is seeded in the sand bed, the final Indian beech seed
It sprouts, then grows up to Indian beech seedling.
2. Indian beech seedling quick efficient breeding method according to claim 1, it is characterised in that: in the step S3
In, the concentration of the gibberellin aqueous solution is preferably 0~200mg/L.
3. Indian beech seedling quick efficient breeding method according to claim 1, it is characterised in that: in the step S3
In, 0~48h of soaking time of the Indian beech seed.
4. Indian beech seedling quick efficient breeding method according to claim 3, it is characterised in that: in the step S3
In, the soaking time of the Indian beech seed is 6~48h.
5. Indian beech seedling quick efficient breeding method according to claim 3, it is characterised in that: in the step S3
In, the soaking time of the Indian beech seed is 24~48h.
6. Indian beech seedling quick efficient breeding method according to claim 1, it is characterised in that: in the step S4
In, the relative humidity of the sand bed is controlled 60%~80%.
7. Indian beech seedling quick efficient breeding method according to claim 1, it is characterised in that: in the step S4
In, the Germination Temperature of the Indian beech seed is 23 DEG C~28 DEG C.
8. Indian beech seedling quick efficient breeding method according to claim 1, it is characterised in that: in the step S2
In, the mode for removing the exocarp of the Indian beech fruit is manual removal.
9. Indian beech seedling quick efficient breeding method according to claim 8, it is characterised in that: in the step S2
In, the mode of the exocarp of the Indian beech fruit is removed by the exocarp of the Indian beech fruit after clear water impregnates 20min
Manual removal.
10. Indian beech seedling quick efficient breeding method according to claim 1, it is characterised in that: in the step S4
In, the time that the Indian beech seed is sprouted is 8~12d.
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| CN201910361093.3A CN110050680A (en) | 2019-04-30 | 2019-04-30 | A kind of Indian beech seedling quick efficient breeding method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777539A (en) * | 2022-12-14 | 2023-03-14 | 江门市新会区林业科学研究所（江门市新会区林业科技推广站） | Buffalo peel tissue culture method |
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2019
- 2019-04-30 CN CN201910361093.3A patent/CN110050680A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777539A (en) * | 2022-12-14 | 2023-03-14 | 江门市新会区林业科学研究所（江门市新会区林业科技推广站） | Buffalo peel tissue culture method |
| CN115777539B (en) * | 2022-12-14 | 2023-09-29 | 江门市新会区林业科学研究所（江门市新会区林业科技推广站） | Tissue culture method for wampee |
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Application publication date: 20190726 |
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