CN110029097A - 一种糖苷水解酶CmNAGase及其克隆表达与应用 - Google Patents
一种糖苷水解酶CmNAGase及其克隆表达与应用 Download PDFInfo
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- CN110029097A CN110029097A CN201910243768.4A CN201910243768A CN110029097A CN 110029097 A CN110029097 A CN 110029097A CN 201910243768 A CN201910243768 A CN 201910243768A CN 110029097 A CN110029097 A CN 110029097A
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- Prior art keywords
- glycoside hydrolase
- ala
- nagase
- cmnagase
- chitin
- Prior art date
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Abstract
本发明公开了一种糖苷水解酶CmNAGase及其克隆表达与应用。将从土壤中筛到的菌株Chitinolyticbacter meiyuanensis SYBC‑H1进行基因组测序分析、克隆CmNAGase、构建重组菌株、表达CmNAGase蛋白、纯化HIS‑TAG，并鉴定重组蛋白具有糖苷水解酶的性质，在高温环境下仍保证良好的酶活性，属于内切酶，且能分别向几丁二糖、几丁三糖、几丁四糖、几丁五糖和几丁六糖的糖链转移一个乙酰氨基基葡萄糖使其糖链长度增加，可通过酶工程、合成生物学对其进行分子改造使该酶的酶促反应的方向朝增加糖链的方向进行，为人工合成几丁寡糖奠定基础。
Description
技术领域
本发明属于基因工程领域，具体涉及一种糖苷水解酶CmNAGase及其克隆表达与应用。
背景技术
几丁质又称甲壳素、壳多糖，是由N-乙酰氨基葡萄糖通过β-1,4-糖苷键连接的聚合物，是地球上除纤维素外含量最高的多聚糖。几丁质广泛存在于虾蟹壳、昆虫的外骨骼和真菌细胞壁中，其最终水解产物N-乙酰氨基葡萄糖可以保护软骨组织及骨关节。几丁质酶可以防治植物的真菌类病害，在食品、医药、农业以及化妆品等行业具有广泛应用。
以几丁质为底物制备N-乙酰氨基葡萄糖(GlcNAc)的方法主要可分为是化学法和酶法，目前工业上多采用化学法，即用强碱脱除乙酰基后，再用强酸水解，使其变成可溶性的氨基葡萄糖(GlcN)。然后再利用化学进行接乙酰基，从而合成GlcNAc。化学法存在反应过程控制较难，伴有副产物产生，且产物的生物活性差等缺陷。此外，在生产过程中涉及到大量强酸强碱，对环境污染严重，三废处理成本高。因此，寻找绿色高效的GlcNAc的制备方法具有重要意义。
几丁质酶是能专一水解几丁质生产GlcNAc及几丁寡糖的糖苷水解酶，因其反应条件温和、控制性强、产物生物活性好且绿色环保，这种生物酶法处理几丁质将成为现代几丁质产业发展方向，具有巨大的发展前景。几丁质酶包括内切几丁质酶(EC 3.2.1.14)，外切几丁质酶(EC 3.2.1.20)和EC 3.2.1.201)和β-N-乙酰氨基葡萄糖苷酶(NAGases，EC3.2.1.52)。几丁质酶将几丁质水解为几丁寡糖，β-N-乙酰氨基葡萄糖苷酶将几丁寡糖降解为 GlcNAc。
GlcNAc已广泛用于生产唾液酸、生物乙醇和单细胞蛋白质；在医药领域可以作为肠胃炎、骨关节炎的药物，还可应用于癌症诊断。NAGases除了水解N-乙酰基几丁寡糖外，其在细胞O-连接的GlcNAc调节数千种细胞内蛋白质的功能，包括在信号转导，基因表达，细胞周期和蛋白酶体降解中起重要作用。
氨基酸序列同源性的比较结果显示NAGases属于糖苷水解酶(GH)3,20,73,84和85个家族(http://www.cazy.org/)。本发明的β-N-乙酰氨基葡萄糖苷酶属于糖苷水解酶GH20家族。
发明内容
针对现有技术的不足，本发明的目的在于提供一种糖苷水解酶CmNAGase及其克隆表达与应用，该β-N-乙酰氨基葡萄糖酶水解几丁寡糖作用，并具有转糖基的作用。
一种糖苷水解酶CmNAGase，其氨基酸序列如SEN ID NO：2所示。
作为改进的是，编码所述氨基酸序列的核苷酸序列如SEN ID NO：1所示。
一种重组表达载体，含有所述编码糖苷水解酶CmNAGase基因的核苷酸序列的重组表达载体。
一种重组菌，含有上述糖苷水解酶CmNAGase基因的表达载体的重组菌。
一种糖苷水解酶CmNAGas基因的克隆表达，包括以下步骤：
步骤1，PCR扩增SEQ ID No.1所示的核苷酸序列；
步骤2，构建重组质粒pET-28a(+)-Cmnagase
将PCR扩增的DNA序列和pET-28a(+)载体用同样的限制性内切酶NdeI和XhoI进行双酶切，酶切产物经回收纯化，用T4DNA连接酶连接纯化的酶切产物，得到质粒载体pET- 28a(+)-Cmnagase；
步骤3，将重组质粒pET-28a(+)-Cmnagase导入大肠杆菌BL21(DE3)，得到含有重组质粒pET-28a(+)-Cmnagase的大肠杆菌BL21(DE3)，命名为重组菌；
步骤4，挑选重组菌的单克隆，摇床过夜培养后，加入诱导剂诱导培养，收集菌液，离心后，收集沉淀；
步骤5，向沉淀中加入缓冲液重悬菌株，再超声裂解后，离心，收集上清后冷冻备备用。
作为改进的是，步骤1中PCR扩增所用的为CmF-5’-CATATGATGAGCCGTCCCGCCGGATC-3’和CmR-5’- CTCGAGTCAGGCGCCCACCTGCACCG-3’。
上述糖苷水解酶CmNAGase在水解几丁寡糖上的应用。
作为改进的是，上述应用中糖苷水解酶CmNAGase的反应温度25～55℃、 pH5.0～7.5。
作为改进的是，所述几丁寡糖为几丁二糖、几丁三糖、几丁四糖、几丁五糖或几丁六糖。
有益效果：
与现有技术相比，本发明糖苷水解酶CmNAGase即β-N-乙酰氨基葡萄糖苷酶高效水解几丁寡糖，对对硝基苯酚-N-乙酰氨葡萄糖的比活力为487U/mg，最适温度40℃、最适pH5.4，在pH5.4～7.5时96h后相对活力保持在70％以上。本发明的酶属于内切糖苷酶，并且具有一定转糖基的作用，它能分别向几丁二糖、几丁三糖、几丁四糖、几丁五糖和几丁六糖的糖链转移一个乙酰氨基葡萄糖使其糖链长度增加，在本发明的基础上，可以通过酶工程、合成生物学对其进行分子改造促使该酶促反应朝增加糖链长度的方向进行，最终可以实现人工合成几丁寡糖。
附图说明
图1为糖苷水解酶CmNAGase的保守结构域示意图；
图2为糖苷水解酶CmNAGase的SDS-PAGE图，其中，M为蛋白maker从上至下分别是180kDa，140kDa，100kDa，75kDa，60kDa，45kDa，35kDa，25kDa，1为重组菌株诱导表达后的粗酶条带，2为粗酶浓缩后条带，3为纯化后的条带；
图3为本发明中温度对糖苷水解酶CmNAGase活性的影响，其中，(A)为最适温度，(B) 为温度稳定性；
图4为本发明中pH对糖苷水解酶CmNAGase活性的影响，其中，(A)为最适pH，(B)为 pH稳定性；
图5为酶CmNAGase的水解模式示意图。
具体实施方式
下面通过实施例对本发明做进一步描述，但不用于限制本发明的保护范围。实施例中的实验方法，如无特殊说明，均为常规方法。
下述实例中所用到的材料、试剂等，如无特殊说明，均可从商业途径获得。
实施例1
菌株来源
(1)本发明所用用菌株C.meiyuanensis SYBC-H1由本实验室筛选并保藏于中国普通微生物菌种保藏管理中心(CGMCC 3438)和美国典型微生物菌种保藏中心(ATCC BAA- 2140)。
实施例2克隆糖苷水解酶基因CmNAGase
(1)糖苷水解酶CmNAGase的基因，其核苷酸序列如SEN ID NO：1所示。
(2)设计PCR扩增引物CmF-5’-CATATGATGAGCCGTCCCGCCGGATC-3’和 CmR-5’-CTCGAGTCAGGCGCCCACCTGCACCG-3’扩增出该基因的全长。
(3)上述PCR扩增的反应条件为：95℃预变性5min；94℃ 30sec、65℃ 30sec、72℃40sec，30个循环；最后在72℃下延伸10min。
(4)用限制性内切酶NdeI和XhoI双酶切PCR扩增产物，回收酶切产物。
(5)用限制性内切酶NdeI和XhoI双酶切载体pET-28(+)a，回收载体骨架(约5400bp)。
(6)向上述步骤的酶切产物和步骤4的载体骨架连接，得到重组质粒pET-28a(+)-Cmnagase。
实施例3糖苷水解酶CmNAGase基因的克隆表达，包括以下步骤：
(1)将重组质粒pET-28a(+)-Cmnagase导入大肠杆菌BL21(DE3)，得到含有重组质粒pET- 28a(+)-Cmnagase的大肠杆菌BL21(DE3)，命名为重组菌。
(2)挑选重组菌(表达含有His标签pET-28a(+)-Cmnagase)的单克隆，接至含100 μg/mL的卡那霉素5mL LB培养基在37℃、200rmp的振荡摇床中过夜培养。
(3)将上述菌液以1:100的体积比接种至100mL LB含100μg/mL卡那霉素的液体培养基，37℃、200rmp的振荡培养至OD600达到0.6-0.8；
(4)然后加入IPTG(诱导剂)至浓度为1mmol/L，20℃、200rmp振荡培养20h。
(5)收集上述菌液到离心管中，4℃，6000rmp/min离心10min，弃上清，收集沉淀。
(6)向细菌沉淀中加入10mLPBS缓冲液重悬菌体。
(7)冰上超声裂解细菌。超声功率为300W，每次超声处理2s，间隔3s，共超声处理10min。
(8)4℃，6000rmp/min离心10min，收集细菌裂解液上清，并置于冰上。
(9)收集的上清液用AKTA蛋白纯化系统和该公司的HIS-TAG柱重组蛋白进行蛋白纯化。纯化后的重组蛋白溶液进行SDS-PAGE，获得85kDa的蛋白条带(结果如图2所示), 且酶活力为487U/mg。
实施例4糖苷水解酶CmNAGase的即N-乙酰氨基葡萄糖苷酶活性的鉴定
利用4-甲基伞形酮-N-乙酰-D葡萄糖胺可以定性的检测糖苷酶的活性，其在紫外下具有荧光。将所纯化得到的的纯酶与4-甲基伞形酮-N-乙酰-D-葡萄糖胺，以体积比1:10的比例反应，在波长450nm处4-甲基伞形酮具有荧光。根据是否在450nm处是否具有荧光可判定其是否具有活性。
实施例5重组酶糖苷水解酶CmNAGase的的最适反应温度的测定
(1)以对硝基苯酚-N-乙酰氨基葡萄糖(pNP-(GlcNAc))为底物时，酶活力通过测量释放的对硝基苯酚的量来的量来计算。1μL的CmNAGase酶加到1mL的0.25mmol/L p-NP-(GlcNAc)溶液在pH为5.4的底物在40℃反应10min，加入1mL NaOH(0.5mol/L)以终止反应。酶活力定义为40℃每分钟酶生成1μmol pNP-(GlcNAc)的酶量。
(2)蛋白含量测定参考考马斯亮蓝法，测得蛋白浓度为1.21g/L。
(3)在反应温度分别为：25℃、30℃、35℃、40℃、45℃、50℃、55 ℃、60℃时，测定CmNAGase的酶反应速率。结果为3次重复取平均值。
(4)结果如图3(A)所示，反应温度为40℃时酶活力最高41.51U，以最高酶反应速率计为100％，计算其余酶活力作图。
实施例6重组酶糖苷水解酶CmNAGase的温度稳定性的测定
(1)将CmNAGase的蛋白液分别置于25℃、30℃、35℃、40℃下孵育12h。
(2)利用对硝基苯酚-N-乙酰氨基葡萄糖(pNP-(GlcNAc))为底物，在40℃时，酶的添加量、反应时间都相同时加入1mL NaOH(0.5mol/L)以终止反应，测定酶 CmNAGase在1～12h的剩余活力。酶活力定义为40℃每分钟酶生成1μmol pNP-(GlcNAc) 的酶量。
(3)蛋白含量测定参考考马斯亮蓝法，测得蛋白浓度为1.21g/L。
(4)将不进行孵育的CmNAGase蛋白液的酶活力计为100％，计算各个个温度预处理的相对酶活(％)，结果如图3(B)所示。
(5)结果表明：CmNAGase在处理2小时后，25℃、30℃、35℃、40℃分别为未预处理的酶反应速率的91％、89％、88％、20％。温度大于等于最适温度即40℃时稳定性急剧下降。
实施例7重组酶糖苷水解酶CmNAGase的最适pH的测定
(1)以对硝基苯酚-N-乙酰氨基葡萄糖(pNP-(GlcNAc))为底物，1μL的CmNAGase酶加到1mL的0.25mmol/L p-NP-(GlcNAc)溶液,在pH＝3.4-9.0的缓冲液中在，40℃下反应 10min，加入1mL NaOH(0.5mol/L)以终止反应测定酶反应。所用缓冲液分别为：pH＝3.0-6.0 时，缓冲液为柠檬酸-柠檬酸钠，pH＝6.0-8.0时，缓冲液为磷酸氢二钠-磷酸二氢钠，pH＝8.0-9.0时，缓冲液为Tris—HCl，pH＝9.0-10，缓冲液甘氨酸-氢氧化钠。
(2)酶活力定义为40℃每分钟酶生成1μmol pNP-(GlcNAc)的酶量。
(3)蛋白含量测定参考考马斯亮蓝法，测得蛋白浓度为1.21g/L。
(4)pH为5.4时酶活力45.38U，将最高酶反应速率计为100％，其他pH下的反应速率除以最高反应速率得到相对应的相对反应速率，结果如图4(A)所示。
(5)结果表明CmNAGase作为糖苷水解酶的最适pH为5.4，在pH＝5.0～7.0范围内活性较高。
实施例8重组酶糖苷水解酶CmNAGase的pH稳定性测试
(1)将CmNAGase蛋白液与不同pH的缓冲液按照等体积混合，25℃孵育12小时；当所用缓冲液分别为pH＝3.0-6.0时，缓冲液为柠檬酸-柠檬酸钠，pH＝6.0-8.0时，缓冲液为磷酸氢二钠-磷酸二氢钠，pH＝8.0-9.0时，缓冲液为Tris—HCl，pH＝9.0-10，缓冲液甘氨酸-氢氧化钠；
(2)将上述预处理后的蛋白液，以0.25mmol/L对硝基苯酚-N-乙酰氨基葡萄糖(pNP-(GlcNAc))为底物，在40℃，pH5.4，以相同的加酶量1μL反应时间10min加入1mL NaOH(0.5mol/L)以终止反应，测定各个时间的剩余活力；
(3)酶活力定义为40℃每分钟酶生成1μmol pNP-(GlcNAc)的酶量；
(4)蛋白含量测定参考考马斯亮蓝法，测得蛋白浓度为1.21g/L；
(5)结果如图4(B)所示，CmNAGase蛋白液保存于pH为5.4、6.0、6.5、7.0、7.5时相对活力(计算方法同实施例6)依次为：76.4％、87.3％、81.9％、70.9％、80.1％；
(6)结果表明CmNAGase蛋白液在pH5.4-7.5范围内较稳定。说明其在中性条件下具有较好的pH稳定性。
实施例9重组酶糖苷水解酶CmNAGase的水解模式的测定
(1)将购买的的几丁寡糖(GlcNAc)2-6标准品配制成10g/L的水溶液；
(2)使用上述(1)中的几丁寡糖为底物，通过高效液相色谱(HPLC)，采用 ALLTECH的Prevail Carbohydrate ES 5u色谱柱分析结果；
(3)流动相成分为乙腈和甲醇，梯度洗脱条件为：0min，75％乙腈，7min，75％乙腈；8min，65％乙腈；15min，65％乙腈；16min，75％乙腈；22min，75％乙腈。柱温箱温度为 40℃，流速为1mL/min。
(4)结果如图5所示，酶CmNAGase从糖链的非还原性末端逐一切割糖链，可作用的底物(GlcNAc)2-6，最终产物为N-乙酰氨基葡萄糖，其中对几丁二糖和几丁三糖的切割效率比较高。并能分别向几丁二糖、几丁三糖、几丁四糖、几丁五糖和几丁六糖的糖链转移一个乙酰氨基葡萄糖使其糖链长度增加。
因此，本发明的酶催化的酶促反应，它能分别向几丁二糖、几丁三糖、几丁四糖、几丁五糖和几丁六糖的糖链转移一个乙酰氨基葡萄糖使其糖链长度增加，在本发明的基础上，可以通过酶工程、合成生物学对其进行分子改造使该酶的酶促反应的方向朝增加糖链的方向进行，最终可以人工合成几丁寡糖。
以上所述的具体实施方式，对本发明的目的、技术方案和有益效果进行了进一步详细说明，所应理解的是，以上所述仅为本发明的具体实施方式而已，并不用于限制本发明，凡在本发明的精神和原则之内，所做的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
序列表
<110> 南京工业大学
<120> 一种糖苷水解酶CmNAGase及其克隆表达与应用
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2511
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
atgagccgtc ccgccggatc gcagctgaag ctcagctggg aatgcgtttc caaccactac 60
gacggcgaca ccttcctcgc ccgcctgacg ctgaccaacc actcggatgc gccgttgacg 120
ggcactgact gggcgctgta tttcaacacc tgccgcaaga tcaagccgga gaccgtgaca 180
ggcggcgtgg cgaccagtca tgtgaatggc gatctgtcca agctcacgcc gaccgccgaa 240
ttcggcacgc tggcgccggg tgaaacgcgg gtgatcgagt acatcggcat cttctgggtg 300
atccaggaaa ccgacgcgcc actgggcttc tatatcgttt atggcgacgg cagcaccacg 360
gcccgcgccg aggccatcgg tgatccgacc atcgtgccgt tcgtgcgccc agagcagcgt 420
aatcgctcgc tggccgacaa ggtgccactc gccacgcccg aatcgcgcta tgccgacaac 480
gagcagctca cgctgctgcc ggccggcgaa gtcggcaaga tcaccccgac gccgctcgaa 540
gcgagctacg gcaacggcac gttcgtgatc gatcgtgaca cgctggtggt gttcccggcc 600
gagctggccg ccgaagccgc cttcctgcgc cagagcctga aggacctgac cggcgccgat 660
ttccatggcg catccgccgg caaaggcatt gtgctgaagc tgggcaagat cgatgtcgcc 720
gagaccggtg cgcttgccga ggcctatacg ttggcggtga atgctggcgg cgtcgagatc 780
gtcggcaatt cgccggccgg cgtgttcaac ggcatccaga gcctgcgcca gctgctgccc 840
gttgccgcct ggaccaatcc gcaggccgtg ctcgccgtgc cgcacgtgca agtgaaggat 900
gcgccgcgct ttgcctatcg cggcatgcac ctggacgtgg gccgcaattt ctcgtccaag 960
gaaaccgtgc tgcgcctgct ggattgcatg gcgctgtaca agctcaacca gttccacttc 1020
cacctcaccg acgacgaagg ctggcgcctg gaaatcccga cgctgcccga gctcaccgaa 1080
atcggcagca agcgcggctt caccatcgac gagcgtgaca acctggtgcc ctgcttcggc 1140
tccggcgccg aggtggaagg ctcgcatggc accggctact acagccgtgc cgatttcatc 1200
gagatcctca agttcgccac cgcgcgccat atcgaagtgg tgcctgaatt cgacgtgccg 1260
ggccacgccc gctccgccat caaggcaatg aatgtgcgct acgagcgcct ggtgaaagcc 1320
ggcaagcagg ccgaagccga gcagtatctg ctcgccgatt tcgatgacgc ctcgaagtac 1380
gaatcggtgc agctgtggca cgacaacgtg atctgcatcg cgatggaagg cggctacaac 1440
ttcatcgaga cggtgatccg cgacgtgaag gtgatgtacg acgaagccgg cgcaccgtgg 1500
accacgctgc ataccggcgg cgacgaagtg ccggccggcg cctgggaagg ctcgccgaag 1560
tgccaggcct tcatgcaggc caacaacctg aagaacaccc gcgagctgct cgactatttc 1620
ctcggccgct accgcgacat cctgaagaag tacaacctga ccttcggcgg ctgggaagaa 1680
attgccctca cccacgagca cgtgaacggc gccaacgtcc acaagcccaa ccccaagttc 1740
gtcaatgcca acttccagcc ctatgtctgg aacaacgtct ggggctgggg ccaggaagac 1800
ttcgcctacc agctggccaa cgccggctac aaggtggtgt tgtgcaacgt caccaacctc 1860
tacttcgatc tggcctacga aaaggacccg aaggagcccg gctactactg gggtggcttc 1920
atcaacaccc gcaaggccta cgaattctgc ccgctcgaca tctacaccac ggccacgctc 1980
aacctgttcg gccatgacct tggcgattcg ctgaaggaca aggcccgcct caccgccgag 2040
ggcaccaaga acgtgatcgg cttgcagggc gaattgtggg cggagaatgc tcgtagtagc 2100
gcccgcgtcg agcaccttgc catgccgcgc atcatcgcgc tgggcgagcg cgcatgggcc 2160
aaggatccgg gctggacctt catcgccgac aaggccgcgc gcgatgcgaa gatggacgcc 2220
gactggaacc agttcgccaa ccgccttggt cagcgcgaac tggcacggct ggacggtttc 2280
ctcggcggct atggctaccg cgtaccggtg ccgggcgcaa agctggaagg cggcaagctc 2340
tacgccaacc tcgaaagccc aggcctgacg ctgcgctaca ccaccgatgg cagcgaaccg 2400
actgctgcct cgtccgccta caccggtccg gtggcggtca gcggcacggt gaccatcgcg 2460
gctttcacca gcaccaaccg tcgcggtcgc gcggtgcagg tgggcgcctg a 2511
<210> 2
<211> 836
<212> PRT
<213> 氨基酸(Abies alba)
<400> 2
Met Ser Arg Pro Ala Gly Ser Gln Leu Lys Leu Ser Trp Glu Cys Val
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Ser Asn His Tyr Asp Gly Asp Thr Phe Leu Ala Arg Leu Thr Leu Thr
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Asn His Ser Asp Ala Pro Leu Thr Gly Thr Asp Trp Ala Leu Tyr Phe
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Thr Ser His Val Asn Gly Asp Leu Ser Lys Leu Thr Pro Thr Ala Glu
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Phe Gly Thr Leu Ala Pro Gly Glu Thr Arg Val Ile Glu Tyr Ile Gly
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Ile Phe Trp Val Ile Gln Glu Thr Asp Ala Pro Leu Gly Phe Tyr Ile
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Val Tyr Gly Asp Gly Ser Thr Thr Ala Arg Ala Glu Ala Ile Gly Asp
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Pro Thr Ile Val Pro Phe Val Arg Pro Glu Gln Arg Asn Arg Ser Leu
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Ala Asp Lys Val Pro Leu Ala Thr Pro Glu Ser Arg Tyr Ala Asp Asn
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Glu Gln Leu Thr Leu Leu Pro Ala Gly Glu Val Gly Lys Ile Thr Pro
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Thr Pro Leu Glu Ala Ser Tyr Gly Asn Gly Thr Phe Val Ile Asp Arg
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Asp Thr Leu Val Val Phe Pro Ala Glu Leu Ala Ala Glu Ala Ala Phe
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Leu Arg Gln Ser Leu Lys Asp Leu Thr Gly Ala Asp Phe His Gly Ala
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Ser Ala Gly Lys Gly Ile Val Leu Lys Leu Gly Lys Ile Asp Val Ala
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Glu Thr Gly Ala Leu Ala Glu Ala Tyr Thr Leu Ala Val Asn Ala Gly
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Gly Val Glu Ile Val Gly Asn Ser Pro Ala Gly Val Phe Asn Gly Ile
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Gln Ser Leu Arg Gln Leu Leu Pro Val Ala Ala Trp Thr Asn Pro Gln
275 280 285
Ala Val Leu Ala Val Pro His Val Gln Val Lys Asp Ala Pro Arg Phe
290 295 300
Ala Tyr Arg Gly Met His Leu Asp Val Gly Arg Asn Phe Ser Ser Lys
305 310 315 320
Glu Thr Val Leu Arg Leu Leu Asp Cys Met Ala Leu Tyr Lys Leu Asn
325 330 335
Gln Phe His Phe His Leu Thr Asp Asp Glu Gly Trp Arg Leu Glu Ile
340 345 350
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Ile Asp Glu Arg Asp Asn Leu Val Pro Cys Phe Gly Ser Gly Ala Glu
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Tyr Leu Leu Ala Asp Phe Asp Asp Ala Ser Lys Tyr Glu Ser Val Gln
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740 745 750
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<210> 3
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
catatgatga gccgtcccgc cggatc 26
<210> 4
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
ctcgagtcag gcgcccacct gcaccg 26
Claims (9)
1.一种糖苷水解酶CmNAGase，其特征在于，其氨基酸序列如SEN ID NO：2所示。
2.根据权利要求1所述的糖苷水解酶CmNAGase，其特征在于，编码所述氨基酸序列的核苷酸序列如SEN ID NO：1所示。
3.一种重组表达载体，其特征在于，含有所述编码糖苷水解酶CmNAGase基因的核苷酸序列的重组表达载体。
4.一种重组菌，含有上述糖苷水解酶CmNAGase基因的表达载体的重组菌。
5.基于权利要求1所述的糖苷水解酶CmNAGase基因的克隆表达，其特征在于，包括以下步骤：
步骤1，PCR扩增SEQ ID No.1所示的核苷酸序列；
步骤2，构建重组质粒pET-28a(+)-Cmnagase
将PCR扩增的DNA序列和pET-28a(+)载体用同样的限制性内切酶进行双酶切，酶切产物经回收纯化，用T4DNA连接酶连接纯化的酶切产物，得到重组质粒；pET-28a(+)-Cmnagase；
步骤3，将重组质粒pET-28a(+)-Cmnagase导入大肠杆菌BL21(DE3)，得到含有重组质粒pET-28a(+)-Cmnagase的大肠杆菌BL21(DE3)，命名为重组菌；
步骤4，挑选重组菌的单克隆，摇床过夜培养后，加入诱导剂诱导培养，收集菌液，离心后，收集沉淀；
步骤5，向沉淀中加入缓冲液重悬菌株，再超声裂解后，离心，收集上清后冷冻备备用。
6.根据权利要求5中所述的糖苷水解酶CmNAGase基因的克隆表达，其特征在于，步骤1中PCR扩增所用的为CmF-5’- CATATGATGAGCCGTCCCGCCGGATC-3’和CmR-5’-CTCGAGTCAGGCGCCCACCTGCACCG-3’。
7.基于权利要求5所得糖苷水解酶CmNAGase在水解几丁寡糖上的应用。
8.根据权利要求7所述的应用，其特征在于，糖苷水解酶CmNAGase的反应温度25~55°C、pH5.0~7.5。
9.根据权利要求7所述的应用，其特征在于，所述几丁寡糖为几丁二糖、几丁三糖、几丁四糖、几丁五糖或几丁六糖。
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| CN113528553A (zh) * | 2021-07-07 | 2021-10-22 | 扬州日兴生物科技股份有限公司 | 一种密码子优化的n-乙酰氨基葡萄糖转移酶基因及其应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101225401A (zh) * | 2008-01-16 | 2008-07-23 | 浙江工商大学 | 一种含有内切几丁质酶基因的重组载体 |
| CN104762307A (zh) * | 2015-04-30 | 2015-07-08 | 大连大学 | 几丁质酶基因chiC及其编码蛋白和应用 |
| CN109182303A (zh) * | 2018-09-21 | 2019-01-11 | 中国农业大学 | 一种巴伦葛兹类芽孢杆菌β-N-乙酰氨基葡萄糖苷酶及其编码基因与应用 |
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| CN101225401A (zh) * | 2008-01-16 | 2008-07-23 | 浙江工商大学 | 一种含有内切几丁质酶基因的重组载体 |
| CN104762307A (zh) * | 2015-04-30 | 2015-07-08 | 大连大学 | 几丁质酶基因chiC及其编码蛋白和应用 |
| CN109182303A (zh) * | 2018-09-21 | 2019-01-11 | 中国农业大学 | 一种巴伦葛兹类芽孢杆菌β-N-乙酰氨基葡萄糖苷酶及其编码基因与应用 |
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| CN112795556A (zh) * | 2021-01-11 | 2021-05-14 | 南京工业大学 | 一种β-N-乙酰氨基葡萄糖苷酶159及其克隆表达与应用 |
| CN113528553A (zh) * | 2021-07-07 | 2021-10-22 | 扬州日兴生物科技股份有限公司 | 一种密码子优化的n-乙酰氨基葡萄糖转移酶基因及其应用 |
| CN113528553B (zh) * | 2021-07-07 | 2023-09-12 | 扬州日兴生物科技股份有限公司 | 一种密码子优化的n-乙酰氨基葡萄糖转移酶基因及其应用 |
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