CN110007038A - A kind of micro-example automatic classification collection device - Google Patents
A kind of micro-example automatic classification collection device Download PDFInfo
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- CN110007038A CN110007038A CN201910377278.3A CN201910377278A CN110007038A CN 110007038 A CN110007038 A CN 110007038A CN 201910377278 A CN201910377278 A CN 201910377278A CN 110007038 A CN110007038 A CN 110007038A
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- automatic classification
- collection device
- example automatic
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- 239000007788 liquid Substances 0.000 claims abstract description 61
- 239000000463 material Substances 0.000 claims abstract description 28
- 239000000725 suspension Substances 0.000 claims abstract description 20
- 239000011159 matrix material Substances 0.000 claims abstract description 18
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 16
- 210000004262 dental pulp cavity Anatomy 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000005057 refrigeration Methods 0.000 claims description 9
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000005485 electric heating Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 238000013519 translation Methods 0.000 claims description 3
- 238000010186 staining Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002789 length control Methods 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/80—Fraction collectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/80—Fraction collectors
- G01N30/82—Automatic means therefor
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of micro-example automatic classification collection devices, including workbench, the workbench is provided with overhead mechanical arm, and the top for being located at the overhead mechanical arm is provided with horizontal suspension, and horizontal suspension is located at the top that pendency liquid blots component, the bottom of the workbench is provided with dot matrix bottle stand, and the inner cavity of dot matrix bottle stand can place several chromatography column feed materials bottles, it is provided with liquid cyclone above the horizontal suspension, delivery pipe, sewer pipe and connecting tube are respectively arranged on the liquid cyclone.The present invention may be implemented increasingly automated; it is collected when operation by the program being pre-designed; tens different sample components can once be collected; it can be with Precise collection in a small amount and micro sample component; the liquid volume of collection can be more much smaller than a common drop; the sample of collection is stored in closed chromatography column feed materials bottle, and closed environment not only can protect sample, can also reduce the chance of sample cross staining.
Description
Technical field
The present invention relates to micro-example collection technique field, specially a kind of micro-example automatic classification collection device.
Background technique
Based on substance in the liquid chromatography technology class experimental analysis that liquid is different with solid interface distribution degree to grow up
Equipment is widely used, many kinds of, such as liquid chromatogram, high pressure liquid chromatography, fast protein liquid chromatography etc., in addition, capillary
Electrophoresis tube enhances separating effect by extra electric field, and flow cytometer then utilizes the principle of similitude to separate different biological cell groups
Point, when using these equipment, it is often necessary to by the particle of component or suspension in the liquid phase of the dissolution after separation in the liquid phase
Again it collects, further to analyze verifying or to be used；
Common practice is to be collected respectively to liquid phase outflow component manually at present, this is interested to one or two of ingredient
When may be implemented, the amount if necessary to the complicated component or component collected is smaller, then be difficult to obtain satisfied effect,
In order to improve working efficiency, more and more experiments use chemical experiment device fraction collector now, collect different liquid
Component, and the original fraction collector for chemical distillation experiment is correspondingly improved, such as Patent No.
A kind of efficient fraction collector of CN201721043125.8, for another example, the design of Patent No. CN108303484A, in fraction
Spray-drying installation is added on the basis of collector and the preparation and enrichment of component may be implemented in vacuum capturing device.Fraction collector
Certain automatic capability is provided, but chemical distillation experiment is very low to separation accuracy requirement, and requires collected sample
Measure it is relatively large, so fraction collector be difficult meet chemistry and Bioexperiment in the growing requirement to the degree of automation
With the requirement accurately recycled to separation component.
Summary of the invention
The purpose of the present invention is to provide a kind of micro-example automatic classification collection devices, to solve in above-mentioned background technique
The problem of proposition.
To achieve the above object, the invention provides the following technical scheme: a kind of micro-example automatic classification collection device, packet
Workbench is included, the workbench is provided with overhead mechanical arm, and the top for being located at the overhead mechanical arm is provided with horizontal suspension,
And horizontal suspension is located at the top that pendency liquid blots component, the bottom of the workbench is provided with dot matrix bottle stand, and dot matrix bottle stand
Inner cavity can place several chromatography column feed materials bottles, be provided with liquid cyclone, the liquid cyclone above the horizontal suspension
On be respectively arranged with delivery pipe, sewer pipe and connecting tube, the other end of the connecting tube is provided with liquid pump, the delivery pipe
The other end extends to more root canal syringe needles that horizontal suspension is connected thereto installation, and more root canal syringe needles are consolidated by needle fixture
It is fixed, and one end of needle fixture is connected with horizontal suspension.
Preferably, the overhead mechanical arm can be moved axially by motor control to X, Y, Z tri-.
Preferably, the needle fixture can take spring, circlip, screw, lock etc..
Preferably, the pendency liquid, which blots, is equipped with the material that strength absorbs liquid on component, the material for absorbing liquid
It can also be infiltrated in advance on material or smear other materials to realize additional function.
Preferably, the pendency liquid blots component as static state, or takes the modes such as rotation, spool, translation, overturning automatic
Replace liquid absorption material.
Preferably, the pendency liquid blots component to fix, and overhead mechanical arm drives syringe needle to press specified time to same position
Point wiping pipe syringe needle；The pendency liquid blots component and is also possible to movement, and Mechanical Driven to a certain site wipes syringe needle.
Preferably, the valve being arranged on the liquid cyclone can take mechanical multiple-way valve, electromagnetism multiple-way valve, electronic more
Port valve etc. can also increase the components such as buffer pool or sample cell on the liquid cyclone.
Preferably, heating element and refrigeration part are provided on the dot matrix bottle stand, the heating element is heating rod, heat
Blower or electric heating etc.；The refrigeration part is refrigeration machine or cooling piece etc..
Preferably, the overhead mechanical arm is completed by itself X-axis and Y-axis movement along the horizontal plane to chromatography column feed materials bottle
Positioning；It can also be completed by moving horizontally dot matrix bottle stand；The mobile dot matrix bottle stand of rotating circular disk can also be passed through.
A kind of micro-example automatic classification collection device proposed by the present invention, beneficial effect are:
1, the present invention may be implemented increasingly automated, and when operation is collected by the program being pre-designed, and can once collect several
Ten different sample components, can be with Precise collection in a small amount with micro sample component, and the liquid volume of collection can be than common
One drop is much smaller, and the sample of collection is stored in closed chromatography column feed materials bottle, and closed environment not only can protect sample,
The chance of sample cross staining can also be reduced；
2, the present invention can be filled with nitrogen or other inert gases for oxidizable ingredient or anaerobe in advance or in time
Into chromatography column feed materials bottle, for needing the ingredient of colour developing or other chemical reactions in real time, developer or reaction solution can be shifted to an earlier date
Or injected in chromatography column feed materials bottle simultaneously, when collection, is more convenient flexibly, practical.
Detailed description of the invention
Fig. 1 is a kind of micro-example automatic classification collection device front view of the present invention.
In figure: 1, overhead mechanical arm, 2, chromatography column feed materials bottle, 3, dot matrix bottle stand, 4, more root canal syringe needles, 5, the fixed dress of syringe needle
Set, 6, pendency liquid blot component, 7, liquid cyclone, 8, workbench, 9, delivery pipe, 10, sewer pipe, 11, connecting tube, 12, liquid
Body pump, 13, horizontal suspension.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of technical solution: a kind of micro-example automatic classification collection device, including work
Make platform 8, overhead mechanical arm 1 is provided on the workbench 8, and is located at overhead mechanical arm top and is provided with horizontal suspension
13, and horizontal suspension 13 is located at the top that pendency liquid blots component 6, pendency liquid, which blots component 6, can be set in any appropriate position
It sets, the bottom of the workbench 8 is provided with dot matrix bottle stand 3, and the inner cavity of dot matrix bottle stand 3 can dispose several chromatography column feed materials bottles 2,
It is provided with liquid cyclone 7 above the horizontal suspension 13, is respectively arranged with delivery pipe 9, sewer pipe on the liquid cyclone 7
10 and connecting tube 11, since the received liquid of micro-example mechanized classification collection device is constant flow, so collecting
After preceding and collection, has a large amount of liquid and need to be discharged at waste liquid, in order to ensure only acquiring useful liquid portion, in more root canals
A liquid cyclone 7 is equipped with before syringe needle 4, liquid cyclone 7 can be set in any appropriate location, and pass through sewer pipe
10 are discharged, and the other end of the connecting tube 11 is provided with liquid pump 12, provide the power of liquid stream by additional liquid pump 12,
Liquid pump 12 may be mounted at some position of the present apparatus, can also be placed on distant location, and the other end of the delivery pipe 9 extends
The more root canal syringe needles 4 installed on to horizontal suspension 13, more root canal syringe needles 4 are the prior art, Patent No. 201920393130.4
Patent sufficiently disclose, sample via Patent No. 201920393130.4 the more root canal syringe needles of confined space, puncture
Chromatography column feed materials bottle cap injects in chromatography column feed materials bottle, and more root canal syringe needles 4 are fixed by needle fixture 5, and the fixed dress of syringe needle
5 settings are set in horizontal 13 end of suspension.
The power supply that electrical parts all in this case are adapted to it is attached by conducting wire, and should be according to practical feelings
Condition selects suitable controller, designs suitable control circuit board, to meet demand for control, specific connection and control sequence,
It should be with reference to following, successive job order completion electric connection, detailed connection means, for skill known in this field between each electrical part
Art.
Preferably, further, the pendency liquid, which blots component 6, to be static state, or takes rotation, volume
The modes such as axis, translation, overturning replace liquid absorption material automatically.
Preferably, further, it can be fixation that the pendency liquid, which blots component 6, and overhead mechanical arm 1 drives
Syringe needle wipes more root canal syringe needles 4 by specified time to same site；The pendency liquid blots component 6 and is also possible to movement, by
Mechanical Driven wipes syringe needle to a certain site, avoids during overhead mechanical arm 1 is mobile, liquid is slowly in more root canal syringe needles
4 tops form pendant drop and may drip at random if this drop is not handled, thus contaminated equipment；It may also be below
The chromatography column feed materials bottle bottleneck of sample is collected in pollution in sampling process, it is even more important that if in waste liquid containing noxious material or
Communicable disease ingredient may then jeopardize experiment operator.This case design not only avoids the cross contamination in sampling, Er Qiebao
The cleaning that more root canal syringe needles 4 are kept in 1 moving process of overhead mechanical arm is demonstrate,proved.
Preferably, further, the valve being arranged on the liquid cyclone 7 can take mechanical multi-pass
Valve, electromagnetism multiple-way valve, electric multi-way valve etc. can also increase the portions such as buffer pool or sample cell on the liquid cyclone 7
Part, what it is due to the processing of micro-example automatic classification collection device is a small amount of even micro-example, it is desirable that all with liquid contact
Component use Precision Machining, reduce dead volume to the greatest extent, thus as far as possible use hydrophobic material, if the amount of each sample collection by
Receiving time length controls, then the sample size of each continuous sampling can be made to differ；Using the big of buffer pool or sample cell
Small to control, being more suitable for different samples has the case where repeated volume, or using other optics, ultrasound, electromagnetism or electricity
The liquid measure technology of son.
Preferably, further, it is provided with heating element and refrigeration part on the dot matrix bottle stand 3, it is described
Heating element is heating rod, air-heater or electric heating etc.；The refrigeration part is refrigeration machine or cooling piece etc., and low temperature can be effective
Saving biological sample and unstable chemical component, high temperature and low temperature can be also used for after collection or simultaneously that catalytic chemistry reacts,
Temperature appropriate can also be used to cultivate certain biomaterials, and batch experiment is directly carried out in chromatography column feed materials bottle.
Preferably, further, the overhead mechanical arm 1 passes through itself X-axis and Y-axis movement along the horizontal plane
Complete the positioning to chromatography column feed materials bottle 2；It can also be completed by moving horizontally dot matrix bottle stand 3；It can also be mobile by rotating circular disk
Dot matrix bottle stand 3 can be used cooperatively by the positioning of battle array bottle stand 3 with overhead mechanical arm 1.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Be interpreted as without departing from the principles and spirit of the present invention to these embodiments carry out it is a variety of variation, modification, replacement and
The possibility of modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of micro-example automatic classification collection device, it is characterised in that: including workbench (8), workbench (8) setting
Have overhead mechanical arm (1), and the top for being located at the overhead mechanical arm (1) is provided with horizontal suspension (13), and horizontal suspension
(13) it is located at the top that pendency liquid blots component (6), the bottom of the workbench (8) is provided with dot matrix bottle stand (3), and dot matrix bottle
The inner cavity of frame (3) can place several chromatography column feed materials bottles (2), be provided with liquid cyclone (7) above the horizontal suspension (13),
Delivery pipe (9), sewer pipe (10) and connecting tube (11), the connecting tube (11) are respectively arranged on the liquid cyclone (7)
The other end be provided with liquid pump (12), the other end of the delivery pipe (9) extends to horizontal suspension (13) and is connected thereto installation
More root canal syringe needles (4), more root canal syringe needles (4) are fixed by needle fixture (5), and the top of needle fixture (5)
End is connected with horizontal suspension (13).
2. a kind of micro-example automatic classification collection device according to claim 1, it is characterised in that: the overhead mechanical
Arm (1) can be moved axially by motor control to X, Y, Z tri-.
3. a kind of micro-example automatic classification collection device according to claim 1, it is characterised in that: the syringe needle is fixed
Device (5) can take spring, circlip, screw, lock etc..
4. a kind of micro-example automatic classification collection device according to claim 1, it is characterised in that: the pendency liquid is inhaled
The material that strength absorbs liquid is installed on dry part (6), can also infiltrate or smear in advance on the material for absorbing liquid
Other materials is to realize additional function.
5. a kind of micro-example automatic classification collection device according to claim 4, it is characterised in that: the pendency liquid is inhaled
Dry part (6) can be static state, or the modes such as rotation, spool, translation, overturning is taken to replace liquid absorption material automatically.
6. a kind of micro-example automatic classification collection device according to claim 4, it is characterised in that: the pendency liquid is inhaled
Dry part (6) can be to fix, and overhead mechanical arm (1) drives syringe needle to wipe more root canal syringe needles by specified time to same site
(4)；The pendency liquid blots component (6) and is also possible to movement, and Mechanical Driven to a certain site wipes syringe needle.
7. a kind of micro-example automatic classification collection device according to claim 1, it is characterised in that: the separating liquid
The valve being arranged on device (7) can take mechanical multiple-way valve, electromagnetism multiple-way valve, electric multi-way valve etc., can also be in the liquid
Increase the components such as buffer pool or sample cell on current divider (7).
8. a kind of micro-example automatic classification collection device according to claim 1, it is characterised in that: the dot matrix bottle stand
(3) heating element and refrigeration part are provided on, the heating element is heating rod, air-heater or electric heating etc.；The refrigeration
Component is refrigeration machine or cooling piece etc..
9. a kind of micro-example automatic classification collection device according to claim 1, it is characterised in that: the overhead mechanical
Arm (1) completes the positioning to chromatography column feed materials bottle (2) by itself X-axis and Y-axis movement along the horizontal plane；It can also be by moving horizontally
Dot matrix bottle stand (3) is completed；It can also be mobile dot matrix bottle stand (3) by rotating circular disk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910377278.3A CN110007038A (en) | 2019-05-07 | 2019-05-07 | A kind of micro-example automatic classification collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910377278.3A CN110007038A (en) | 2019-05-07 | 2019-05-07 | A kind of micro-example automatic classification collection device |
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| CN110007038A true CN110007038A (en) | 2019-07-12 |
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| CN201910377278.3A Pending CN110007038A (en) | 2019-05-07 | 2019-05-07 | A kind of micro-example automatic classification collection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113189212A (en) * | 2021-03-24 | 2021-07-30 | 上海星猫仪器设备有限公司 | Chromatograph all-in-one machine |
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|---|---|---|---|---|
| DE19852947A1 (en) * | 1998-11-12 | 2000-05-18 | Univ Schiller Jena | Micro-liter automatic dispensing apparatus comprises needle moving vertically to penetrate cover over micro-titration plate, to dispense liquid into cells |
| CN102124329A (en) * | 2008-08-19 | 2011-07-13 | 株式会社岛津制作所 | Split liquid chromatographic apparatus |
| CN104634912A (en) * | 2015-01-20 | 2015-05-20 | 大连依利特分析仪器有限公司 | Liquid phase chromatographic sample manager |
| CN104897827A (en) * | 2015-06-16 | 2015-09-09 | 常州三泰科技有限公司 | Preparative type fraction collector avoiding sample loss |
| CN205120676U (en) * | 2015-10-16 | 2016-03-30 | 广东省肇庆市质量计量监督检测所 | Automatic priming device suitable for gas liquid chromatogram - mass spectrography |
| US20160288016A1 (en) * | 2015-04-03 | 2016-10-06 | Shimadzu Corporation | Fraction collector |
| CN108303484A (en) * | 2018-01-23 | 2018-07-20 | 苏州大学 | A kind of liquid chromatogram fraction collector and application method |
-
2019
- 2019-05-07 CN CN201910377278.3A patent/CN110007038A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19852947A1 (en) * | 1998-11-12 | 2000-05-18 | Univ Schiller Jena | Micro-liter automatic dispensing apparatus comprises needle moving vertically to penetrate cover over micro-titration plate, to dispense liquid into cells |
| CN102124329A (en) * | 2008-08-19 | 2011-07-13 | 株式会社岛津制作所 | Split liquid chromatographic apparatus |
| US20110184658A1 (en) * | 2008-08-19 | 2011-07-28 | Shimadzu Corporation | Preparative Liquid Chromatograph System |
| CN104634912A (en) * | 2015-01-20 | 2015-05-20 | 大连依利特分析仪器有限公司 | Liquid phase chromatographic sample manager |
| US20160288016A1 (en) * | 2015-04-03 | 2016-10-06 | Shimadzu Corporation | Fraction collector |
| CN104897827A (en) * | 2015-06-16 | 2015-09-09 | 常州三泰科技有限公司 | Preparative type fraction collector avoiding sample loss |
| CN205120676U (en) * | 2015-10-16 | 2016-03-30 | 广东省肇庆市质量计量监督检测所 | Automatic priming device suitable for gas liquid chromatogram - mass spectrography |
| CN108303484A (en) * | 2018-01-23 | 2018-07-20 | 苏州大学 | A kind of liquid chromatogram fraction collector and application method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113189212A (en) * | 2021-03-24 | 2021-07-30 | 上海星猫仪器设备有限公司 | Chromatograph all-in-one machine |
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Application publication date: 20190712 |
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