CN110002919A - A kind of fulvic acid chelated zinc bio-fertilizer and its application in walnut plantation - Google Patents
A kind of fulvic acid chelated zinc bio-fertilizer and its application in walnut plantation Download PDFInfo
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- CN110002919A CN110002919A CN201910364536.4A CN201910364536A CN110002919A CN 110002919 A CN110002919 A CN 110002919A CN 201910364536 A CN201910364536 A CN 201910364536A CN 110002919 A CN110002919 A CN 110002919A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The present invention discloses a kind of fulvic acid chelated zinc bio-fertilizer and its application in walnut plantation.The present invention prepares fulvic acid chelated zinc bio-fertilizer by blending using mix bacterium agent and fulvic acid chelated zinc solution, rotten acid fertilizer of chelated zinc rapid mass propagation of microorganism after walnut foliage applying, it being capable of degrading pesticide fertilizer residue, eliminate heavy metal pollution, the strong complexing power and Zinc Ions Chelated of fulvic acid is utilized in fulvic acid chelated zinc bio-fertilizer, it is more advantageous to the absorption of Walnut, improve the utilization rate of zinc fertilizer, improve walnut product quality, reduce pesticide residue, Zn content is higher in walnut kernel, realizes that walnut zinc-rich promotes the purpose of health-care efficacy.
Description
Technical field
The present invention relates to chemical fertilizer fields, specifically, the present invention relates to fulvic acid chelated zinc bio-fertilizer and its in walnut kind
The technical field of application in plant.
Background technique
Walnut (Juglans regiaL.) is important woody oil tree species, economic, ecology and society's effect with higher
Benefit vigorously supports woody oleiferous plants industry recently as country, and the market demand gradually increases, and the cultivated area of walnut is continuous
Expand.Water and fertilizer management is the key factor for realizing Walnut, and applying fertilizers scientifically is that raising walnut yield is relatively easy effective
Approach, the nutritive element content in blade are able to reflect tree body to the demand utilization situation of various nutritional ingredients, to seeking in blade
It supports element and carries out dynamic analysis, can be used as a kind of foundation of fertilising.Mineral needed for blade not only provides fruit development
Nutrition, while the mineral nutrient that soil can be provided is in store.With the research application of foliar fertilizer, fertilizer type is constantly rich
Richness updates, and foliar fertilizer and its application effect achieve huge progress, currently, domestic scholars are in relation to foliage dressing to the shadow of walnut
It is very few to ring research report, and is concentrated mainly on that Juglans regia trees spray, different times respective concentration sprays comparison growth result etc. side
Face.
Zinc is one of the essential trace elements of the human body, zinc be growth in humans's development, reproduction heredity, immune and endocrine system, nerve,
Essential substance in the important physiology course such as body fluid, zinc-deficiency can have an adverse effect to each system of human body.Grain, water
The zinc such as fruit, crop source is mainly chemical fertilizer, and zinc supplementation is an indirect raising human body Zn content important channel in chemical fertilizer.It is previous to solve zinc
3 kinds of main policies of malnutritive problem are, first is that medicament supplements, such as sell Zinc Gluuconate Oral Solution, medicament in the market
It is very useful for supplementing to the rapid nutrition condition for improving nutritional deficiency individual, but cost is larger, and people are acceptable poor to it；
Second is that Zn-ef ficiency, is such as added into cooked rice by condensed food in the form of additive, condensed food will generally also increase throwing
Enter, and the perception of food and flavor can be made to change, is not easy to be received, meanwhile, the safety of nourishing reinforced food is not allowed to neglect yet
Depending on；Third is that varying the diet, increase the intake of the foods such as meat products, marine product.
By Cultural practices, the Zn content of crop edible portion is improved, it, can be in the case where not changing diet
Micronutrient needed for providing people by zinc-rich food steadily in the long term.Currently, for the research report of zinc-rich walnut cultivation
Road is very few.
Summary of the invention
It is mentioned for having no related in the prior art in walnut planting process by applying fulvic acid chelated zinc bio-fertilizer
The state of the art of high walnut edible part Zn content, the present invention is intended to provide a kind of fulvic acid chelated zinc bio-fertilizer and its in walnut
Application in plantation.The present invention prepares fulvic acid chelated zinc life by blending using mix bacterium agent and fulvic acid chelated zinc solution
Object fertilizer, fulvic acid chelated zinc bio-fertilizer rapid mass propagation of microorganism after walnut foliage applying, can degrading pesticide chemical fertilizer it is residual
It stays, eliminates heavy metal pollution, fulvic acid chelated zinc bio-fertilizer is utilized the strong complexing power and Zinc Ions Chelated of fulvic acid, more has
Conducive to the absorption of Walnut, the utilization rate of zinc fertilizer is improved, improves walnut product quality, reduces pesticide residue, in walnut kernel
Zn content is higher, realizes that walnut zinc-rich promotes the purpose of health-care efficacy.
The present invention uses main technical solution:
The present invention specifically provides a kind of preparation method of fulvic acid chelated zinc bio-fertilizer, and steps are as follows for specific preparation method:
(1) fulvic acid chelated zinc solution preparation: according to parts by weight than meter, by 1-2 parts of biochemical fulvic acid, zinc sulfate 4-6
Part, 9-11 parts of potassium dihydrogen phosphate, 45-55 parts of water are uniformly mixed, and put into first order reaction kettle, adjust pH6.0-7.0, reaction temperature
Degree is 30-40 DEG C, reaction time 40-60min, is transferred in second order reaction kettle after first order reaction, reaction temperature 50-70
DEG C, reaction time 20-30min is transferred in third-order reaction kettle after second order reaction, and reaction temperature is 75-85 DEG C, when reaction
Between for 15-25min by the chelatropic reaction of third-order reaction kettle obtain fulvic acid chelated zinc solution；
(2) prepared by mix bacterium agent: selecting bacillus pumilus, Methylotrophic bacillus, red pseudomonas, AVM hereinafter chain
Yeast and mold is mixed, and mix bacterium agent is prepared, and detection viable count reaches 8.6 × 1010cfu/ml；
(3) preparation of fulvic acid chelated zinc bio-fertilizer: according to parts by weight than meter, fulvic acid chela prepared by step (1)
Fertilizer of chelated zinc mixed liquor is made after mixing in 1-2 parts of mix bacterium agent for closing zinc solution 6-8 parts and step (2) preparation, by weight
Percentages are measured, 1-3% xanthan gum, 1-2% chitin are added into fertilizer of chelated zinc mixed liquor, thallus is played and suspends and be retained and make
With being mixed evenly, prepare fulvic acid chelated zinc bio-fertilizer.
Preferably, of the invention, the fulvic acid chelated zinc solution preparation, according to parts by weight than meter, by biochemical fulvic acid 1
Part, 5 parts of zinc sulfate, 10 parts of potassium dihydrogen phosphate, 60 parts of water are uniformly mixed, and put into first order reaction kettle, adjust pH 6.0, reaction
Temperature is 35 DEG C, reaction time 50min, is transferred in second order reaction kettle after first order reaction, and reaction temperature is 60 DEG C, reaction
Time is 25min, is transferred in third-order reaction kettle after second order reaction, and reaction temperature is 80 DEG C, reaction time 20min, warp
The chelatropic reaction for crossing third-order reaction kettle obtains fulvic acid chelated zinc solution.
Preferably, of the invention, the mix bacterium agent preparation selects bacillus pumilus according to volume basis: red vacation unit cell
Bacterium: Methylotrophic bacillus: Avid kyowamycin: saccharomycete=4:5:5:2:4 is mixed, prepares mix bacterium agent,
Detection viable count reaches 8.6 × 1010cfu/ml。
Preferably, of the invention, the fulvic acid chelated zinc bio-fertilizer, according to parts by weight than meter, by fulvic acid chelated zinc
7 parts and 1 part of mix bacterium agent of solution, after mixing, fertilizer of chelated zinc mixed liquor is made, by weight percentage, to fertilizer of chelated zinc
2% xanthan gum, 1.5% chitin are added in mixed liquor, plays thallus suspension and water retention, is mixed evenly, preparation obtains
Obtain fulvic acid chelated zinc bio-fertilizer.
The present invention, the biochemical fulvic acid, steps are as follows for specific preparation method:
(1) feedstock processing: according to parts by weight than meter, 11-13 parts of stalk, 4-6 parts of wheat bran, 2-4 parts of sawdust, walnut is green
2-4 parts of skin, 1-2 parts of thin skin walnut shell, 1-2 parts of urea are uniformly mixed, Mechanical Crushing, rear that clear water 30-40 parts of mixing is added, and are obtained
Obtain mixed raw material；
(2) first stage ferments: by weight percentage, in the mixed raw material obtained to step (1), the first stage is added
Ferment mix bacterium agent 5%, when material temperature rises to 65 DEG C or more, maintains 48h, during which turns 2 times, obtains first stage fermentation
Object；
(3) second stage is fermented: by weight percentage, in the first stage fermentation material obtained to step (2), step is added
Suddenly the addition mixed raw material 20% of (1) preparation and second stage fermentation mix bacterium agent 5%, are uniformly mixed fermentation 96h, during which turn over
It mixes 4 times, obtains second stage fermentation material；
(4) the second stage fermentation material of step (3) preparation the preparation of biochemical fulvic acid: is squeezed into the suspension being obtained by filtration
The biochemical fulvic acid that concentration is 65% can be obtained in liquid, concentrated purification.
The present invention, the first stage fermentation mix bacterium agent, according to volume basis, chain acidophil: bacillus pumilus: white
Rotten bacterium: Clostridium thermocellum=1:2:1:1.
The present invention, the second stage fermentation mix bacterium agent, according to volume basis, bacillus subtilis: aspergillus niger: trichoderma
Bacterium=2:2:1.
Preferably, of the invention, the biochemical fulvic acid raw material, according to parts by weight than meter, by 12 parts of stalk, 5 parts of wheat bran,
3 parts of sawdust, 3 parts of green peel of walnut, 1 part of thin skin walnut shell, 1 part of urea are uniformly mixed, Mechanical Crushing, and 35 parts of clear water of rear addition is mixed
It closes, obtains mixed raw material.
The present invention, the bacillus pumilus, Methylotrophic bacillus, red pseudomonas, Avid kyowamycin, yeast
Bacterium, whiterot fungi, Clostridium thermocellum, bacillus subtilis, aspergillus niger and Trichoderma, fermentation liquid detection viable count are not less than 1
×109cfu/ml。
Further, the present invention provides the application for applying a kind of above-mentioned fulvic acid chelated zinc bio-fertilizer in walnut plantation.
By utilizing bacillus pumilus, Methylotrophic bacillus, red pseudomonas, Avid kyowamycin, saccharomycete
The compatible mix bacterium agent of five kinds of bacterium and fulvic acid chelated zinc solution mix the fulvic acid chelated zinc bio-fertilizer of preparation, Yi Jili
Matched with 7 kinds of chain acidophil, bacillus pumilus, whiterot fungi, Clostridium thermocellum, bacillus subtilis, aspergillus niger, Trichoderma bacterium
In the biochemical fulvic acid of 5 Fermentation Fulvic Acid's mixed raw materials preparation, the specificity and complexity of microorganism fungus kind, by various differences
Strain can compound compatible use, consider the safety of composite bacteria, be especially applied to prepare biochemical fulvic acid and preparation
In fulvic acid chelated zinc bio-fertilizer require a large amount of infrastest verifying, the present invention is based on basic research early period accumulation, pass through
Test is compounded using a large amount of different strains, it was demonstrated that the present invention is mixed with using mixed bacteria and fulvic acid chelated zinc solution
Fulvic acid chelated zinc bio-fertilizer is applied in walnut plantation, and the rapid mass propagation of microorganism after walnut foliage applying can drop
Agriculture chemical residual is solved, heavy metal pollution is eliminated, the strong complexing power and zinc of fulvic acid is utilized in fulvic acid chelated zinc bio-fertilizer
Ion chelating is more advantageous to the absorption of Walnut, improves the utilization rate of zinc fertilizer, improves walnut product quality, and it is residual to reduce pesticide
It stays, Zn content is higher in walnut kernel, realizes that walnut zinc-rich promotes the purpose of health-care efficacy.
In the present invention, bacillus pumilus (Bacillus pumilus), the Methylotrophic bacillus of selection
(Bacillus methylotrophicus), saccharomycete (saccharomyce), red pseudomonas
(Rhodopseudomonas), Avid kyowamycin (Streptomyces avermit ilis), chain acidophil
(Streptacidiphilus), Clostridium thermocellum (Clostridium the rmocellum), whiterot fungi
(Phanerochaetc chrysosporium), bacillus subtilis (Bacillus subtilis), aspergillus niger
(Aspergillus), a kind of bacterium of Trichoderma (Trichodermaspp.) ten is all the known strain of the common public, and this field is general
Logical technical staff can be bought by public's channel and be obtained, and it is normal that this field all can be used in the condition of culture and culture medium of a kind of ten bacterium
Appear in the newspapers acquisition.
Emphasis of the present invention is to be experimentally confirmed to be answered using a kind of this ten bacterium according to technical solution provided by the invention
Match, prepare biochemical fulvic acid, or blend with fulvic acid chelated zinc solution, then mix with thallus suspending agent and water-retaining agent, uses
Specific compatibility and preparation process, prepare fulvic acid chelated zinc bio-fertilizer, although a kind of this ten bacterium is ripe for the common public
Know strain, but a kind of this ten strain is not provided in the prior art and is compounded, prepares fulvic acid chelated zinc bio-fertilizer, solve
It is used in walnut plantation, improves walnut edible part Se content, improve walnut product quality, realize walnut zinc-rich high yield mesh
's；And the present invention utilize microorganism fungus kind specificity and complexity, by a variety of different strains can compound compatible use, lead to
The blending of crossing various strains, compatibility in conjunction with each strain attribute, the safety of the composite bacteria of consideration and other fertilizer
Compatibility demand, it is creative in the preparation of fulvic acid chelated zinc solution and mix bacterium agent experiments prove that technique, system
Standby fulvic acid chelated zinc bio-fertilizer has effects that dramatically increase walnut edible part Zn content, be shown in walnut plantation application
Write technical effect outstanding.
By implementing the specific summary of the invention of the present invention, can achieve it is specific following the utility model has the advantages that
(1) a kind of fulvic acid chelated zinc bio-fertilizer prepared by the present invention, passes through fulvic acid chelated zinc solution and mix bacterium agent
The fulvic acid chelated zinc bio-fertilizer being mixed with, the phase of bearing fruit are sprayed on Walnut Leaves blade positive and negative, fulvic acid chelated zinc biology
It is 2.96mg/kg that fertile 1.5kg/ mus, which sprays Zn content in rear Walnut Fruit, and compared with the control, Zn content improves in Walnut Fruit
44.6%, compared with commercially available soil fertilization material, in Walnut Fruit Zn content improve 37.5%, with it is commercially available spray foliar fertilizer compared with, core
Zn content improves 28.7% in Peach fruits, and strong complexing power and the zinc ion chela of fulvic acid is utilized in fulvic acid chelated zinc bio-fertilizer
It closes, is more advantageous to the absorption of Walnut, improve the utilization rate of zinc fertilizer, improve walnut product quality, reduce pesticide residue, walnut
Zn content is higher in kernel, realizes that walnut zinc-rich promotes the purpose of health-care efficacy, obtains good technical effect, with extensive
Actual value.
(2) a kind of fulvic acid chelated zinc bio-fertilizer prepared by the present invention, mix bacterium agent are sought by bacillus pumilus, methyl
It is compatible to support type bacillus, red pseudomonas, Avid kyowamycin and five kinds of bacterium of saccharomycete, detection viable count reaches 8.6 ×
1010Cfu/ml, the rapid mass propagation of microorganism after rotten sour fertilizer of chelated zinc is in walnut foliage applying in fertilizer, can degrade agriculture
Medicine fertilizer residue, eliminates heavy metal pollution, improves Walnut Leaves indirectly and faces the positive effect that Zn-ef ficiency absorbs.
(3) a kind of fulvic acid chelated zinc bio-fertilizer prepared by the present invention passes through addition xanthan gum and crust in preparation process
Element, play thallus suspend and water retention, after application blade can casting film-forming, on the one hand be conducive to fulvic acid chelated zinc bio-fertilizer
It is kept for a long time on blade face, the ability for increasing blade and absorbing and adsorbing dust is on the other hand flowed by air, for planting
The protection of object growth complete period blade.
(4) a kind of fulvic acid chelated zinc bio-fertilizer prepared by the present invention, biochemical fulvic acid is by chain acidophil, short and small gemma
It ferments in the investment mixed raw material of bacillus, whiterot fungi, Clostridium thermocellum, bacillus subtilis, aspergillus niger, Trichoderma in batches,
The fulvic acid content of the biochemical fulvic acid of preparation is 65%, and adjusting pH is 6.0, is easy to carry out chela with zinc sulfate, potassium dihydrogen phosphate
Close, the biochemical fulvic acid of preparation directly can provide organic fertilizer abundant for walnut crop, for microorganism provide effective carrier with
Nutriment makes the nutrient in the dedicated fulvic acid chelated zinc bio-fertilizer of peach stablize release, improves walnut edible part Zn content, change
Kind walnut quality.
Detailed description of the invention
Nothing
Specific embodiment
In the following, illustrating the present invention for embodiment, still, the present invention is not limited to following embodiments.
All raw and auxiliary materials selected in the present invention, and the Spawn incubation method selected all are well known in the art selection
, the % that is related to is weight percentage in the present invention, unless otherwise indicated except.
Embodiment one: the preparation of fulvic acid chelated zinc bio-fertilizer
The present invention specifically provides a kind of preparation method of fulvic acid chelated zinc bio-fertilizer, and steps are as follows for specific preparation method:
(1) fulvic acid chelated zinc solution preparation: according to parts by weight than meter, by 1-2 parts of biochemical fulvic acid, zinc sulfate 4-6
Part, 9-11 parts of potassium dihydrogen phosphate, 45-55 parts of water are uniformly mixed, and put into first order reaction kettle, adjust pH6.0-7.0, reaction temperature
Degree is 30-40 DEG C, reaction time 40-60min, is transferred in second order reaction kettle after first order reaction, reaction temperature 50-70
DEG C, reaction time 20-30min is transferred in third-order reaction kettle after second order reaction, and reaction temperature is 75-85 DEG C, when reaction
Between for 15-25min by the chelatropic reaction of third-order reaction kettle obtain fulvic acid chelated zinc solution；
(2) prepared by mix bacterium agent: selecting bacillus pumilus, Methylotrophic bacillus, red pseudomonas, AVM hereinafter chain
Yeast and mold is mixed, and mix bacterium agent is prepared, and detection viable count reaches 8.6 × 1010cfu/ml；
(3) preparation of fulvic acid chelated zinc bio-fertilizer: according to parts by weight than meter, fulvic acid chela prepared by step (1)
Fertilizer of chelated zinc mixed liquor is made after mixing in 1-2 parts of mix bacterium agent for closing zinc solution 6-8 parts and step (2) preparation, by weight
Percentages are measured, 1-3% xanthan gum, 1-2% chitin are added into fertilizer of chelated zinc mixed liquor, thallus is played and suspends and be retained and make
With being mixed evenly, prepare fulvic acid chelated zinc bio-fertilizer.
Preferably, of the invention, the fulvic acid chelated zinc solution preparation, according to parts by weight than meter, by biochemical fulvic acid 1
Part, 5 parts of zinc sulfate, 10 parts of potassium dihydrogen phosphate, 60 parts of water are uniformly mixed, and put into first order reaction kettle, adjust pH 6.0, reaction
Temperature is 35 DEG C, reaction time 50min, is transferred in second order reaction kettle after first order reaction, and reaction temperature is 60 DEG C, reaction
Time is 25min, is transferred in third-order reaction kettle after second order reaction, and reaction temperature is 80 DEG C, reaction time 20min, warp
The chelatropic reaction for crossing third-order reaction kettle obtains fulvic acid chelated zinc solution.
Preferably, of the invention, the mix bacterium agent preparation selects bacillus pumilus according to volume basis: red vacation unit cell
Bacterium: Methylotrophic bacillus: Avid kyowamycin: saccharomycete=4:5:5:2:4 is mixed, prepares mix bacterium agent,
Detection viable count reaches 8.6 × 1010cfu/ml。
Preferably, of the invention, the fulvic acid chelated zinc bio-fertilizer, according to parts by weight than meter, by fulvic acid chelated zinc
7 parts and 1 part of mix bacterium agent of solution, after mixing, fertilizer of chelated zinc mixed liquor is made, by weight percentage, to fertilizer of chelated zinc
2% xanthan gum, 1.5% chitin are added in mixed liquor, plays thallus suspension and water retention, is mixed evenly, preparation obtains
Obtain fulvic acid chelated zinc bio-fertilizer.
Embodiment two: the preparation of biochemical fulvic acid
Biochemical fulvic acid, steps are as follows for specific preparation method:
(1) feedstock processing: according to parts by weight than meter, 11-13 parts of stalk, 4-6 parts of wheat bran, 2-4 parts of sawdust, walnut is green
2-4 parts of skin, 1-2 parts of thin skin walnut shell, 1-2 parts of urea are uniformly mixed, Mechanical Crushing, rear that clear water 30-40 parts of mixing is added, and are obtained
Obtain mixed raw material；
(2) first stage ferments: by weight percentage, in the mixed raw material obtained to step (1), the first stage is added
Ferment mix bacterium agent 5%, when material temperature rises to 65 DEG C or more, maintains 48h, during which turns 2 times, obtains first stage fermentation
Object；
(3) second stage is fermented: by weight percentage, in the first stage fermentation material obtained to step (2), step is added
Suddenly the addition mixed raw material 20% of (1) preparation and second stage fermentation mix bacterium agent 5%, are uniformly mixed fermentation 96h, during which turn over
It mixes 4 times, obtains second stage fermentation material；
(4) the second stage fermentation material of step (3) preparation the preparation of biochemical fulvic acid: is squeezed into the suspension being obtained by filtration
The biochemical fulvic acid that concentration is 65% can be obtained in liquid, concentrated purification.
The present invention, the first stage fermentation mix bacterium agent, according to volume basis, chain acidophil: bacillus pumilus: white
Rotten bacterium: Clostridium thermocellum=1:2:1:1.
The present invention, the second stage fermentation mix bacterium agent, according to volume basis, bacillus subtilis: aspergillus niger: trichoderma
Bacterium=2:2:1.
Preferably, of the invention, the biochemical fulvic acid raw material, according to parts by weight than meter, by 12 parts of stalk, 5 parts of wheat bran,
3 parts of sawdust, 3 parts of green peel of walnut, 1 part of thin skin walnut shell, 1 part of urea are uniformly mixed, Mechanical Crushing, and 35 parts of clear water of rear addition is mixed
It closes, obtains mixed raw material.
The present invention, the bacillus pumilus, Methylotrophic bacillus, red pseudomonas, Avid kyowamycin, yeast
Bacterium, whiterot fungi, Clostridium thermocellum, bacillus subtilis, aspergillus niger and Trichoderma, fermentation liquid detection viable count are not less than 1
×109cfu/ml。
Embodiment three: biochemical fulvic acid preparation condition optimization experiment
(1) biochemical fulvic acid feedstock optimization
It by stalk, wheat bran, sawdust, green peel of walnut, thin skin walnut shell, urea, is mixed according to different ratio, machinery is broken
Broken, rear that 35kg clear water is added, being added the first stage into mixed raw material ferments mix bacterium agent 3%, when material temperature rise to 65 DEG C with
When upper, 48h was maintained, is during which turned 2 times, mixed raw material 20% and second stage is added to feed liquid again after fermentation in the first stage
Ferment mix bacterium agent 3%, is uniformly mixed fermentation 96h, during which turns 4 times, obtains second stage fermentation material；First stage fermentation is mixed
Combined bacteria agent ratio is chain acidophil: bacillus pumilus: whiterot fungi: Clostridium thermocellum=1:1:1:1, second stage fermentation mixing
Microbial inoculum is bacillus subtilis: aspergillus niger: Trichoderma=1:1:1.Detect fulvic acid content in second stage fermentation material.
The measurement of fulvic acid content: fulvic acid is extracted from second stage fermentation material, dichromic acid is used in strongly acidic solution
Oxidation of coal in fulvic acid at carbon dioxide, is used water as Extraction solvent by potassium, according to potassium bichromate consumption and fulvic acid
Carbon ratio calculates content.
Table 1: influence of the different ratios of raw materials to fulvic acid content in second stage fermentation material
In the optimization experiment of fulvic acid fermentation raw material, as a result ginseng is shown in Table 1, by stalk, wheat bran, sawdust, green peel of walnut, thin
Skin walnut shell, urea, are mixed according to different ratio, and the Huang wherein obtained after the fermenting raw materials of F group material combination proportion is rotten
Acid content highest is 20.67%, therefore selects more excellent combination: stalk 12kg, wheat bran 5kg, sawdust 3kg, green peel of walnut 3kg, thin
Skin walnut shell 1kg, urea 1kg carry out fulvic acid fermentation as raw material.
(2) fermentation mix bacterium agent optimization
By stalk 12kg, wheat bran 5kg, sawdust 3kg, green peel of walnut 3kg, thin skin walnut shell 1kg, urea 1kg, mixed
It closes, Mechanical Crushing, it is rear that 35kg clear water is added, first stage fermentation mix bacterium agent 3% is added into mixed raw material, when material temperature rises
When to 65 DEG C or more, 48h is maintained, is during which turned 2 times, 20% He of mixed raw material is added to feed liquid again after fermentation in the first stage
Second stage fermentation mix bacterium agent 3%, is uniformly mixed fermentation 96h, during which turns 4 times, obtains second stage fermentation material, and detection is given birth to
Change fulvic acid content；First stage and second stage fermentation mix bacterium agent are matched according to ratio shown in table 2.
Table 2: fermentation mix bacterium agent different ratio fermentation obtains biochemical fulvic acid content
By mixing between first stage mix bacterium agent and second stage mix bacterium agent according to different proportion, fermentation is obtained
The fulvic acid obtained, experimental result ginseng is shown in Table 2, wherein the first stage mix bacterium agent and second being mixed in C group ratio
Fulvic acid content highest in the fulvic acid that the fermentation of stage mix bacterium agent obtains is 22.34%, hence it is evident that is matched higher than other combination microbial inoculums
Than by the experiment, selecting optimal one stage fermentation mix bacterium agent of microbial inoculum is chain acidophil: bacillus pumilus: whiterot fungi: heat
Fiber clostridium=1:2:1:1, second stage ferment mix bacterium agent for bacillus subtilis: aspergillus niger: Trichoderma=2:2:1 is carried out
Fulvic acid fermentation.
(4) orthogonal experiment optimizes
First stage fermenting agent additive amount, first stage fermentation time, second stage zymophyte are probed into this experiment respectively
The influence of agent additive amount, second stage fermentation time to fulvic acid content in biochemical fulvic acid.On the basis of experiment of single factor
The experiment of four factors, three horizontal quadrature is carried out, orthogonal test factor and water-glass are shown in Table 3.
Table 3: orthogonal test factor and water-glass
Orthogonal optimization different fermentations condition is to fulvic acid content test result in biochemical fulvic acid and analysis referring to 4 institute of table
Show.
Table 4: orthogonal experiments and analysis
From table 4, it can be seen that in the range of experimental design, first stage fermenting agent additive amount, first stage fermentation
Influence of the time to fulvic acid content in biochemical fulvic acid is maximum.Obtaining optimum optimizing condition in conjunction with table 3 and table 4 is A2B2C2D2,
That is first stage fermenting agent additive amount 5%, first stage fermentation time 48h, second stage fermenting agent additive amount 5%,
When two-stage fermentation time when 96h, fulvic acid content highest in biochemical fulvic acid.
By the above optimum experimental obtain biochemical fulvic acid the preparation method comprises the following steps: by stalk 12kg, wheat bran 5kg, sawdust 3kg,
Green peel of walnut 3kg, thin skin walnut shell 1kg, urea 1kg, the rear clear water 35kg that is added are mixed, acquisition mixed raw material, in mixed raw material,
First stage fermentation mix bacterium agent 5% is added, the first stage ferments mix bacterium agent for chain acidophil: bacillus pumilus: white rot
Bacterium: Clostridium thermocellum=1:2:1:1 maintains 48h, during which turns 2 times when material temperature rises to 65 DEG C or more, obtains the first rank
Section fermentation material, then mixed raw material 20% and second stage fermentation mix bacterium agent 5%, two-stage hair is added to first stage fermentation material
Ferment mix bacterium agent is bacillus subtilis: aspergillus niger: Trichoderma=2:2:1, is uniformly mixed fermentation 96h, during which turns 4 times, obtain
Obtain second stage fermentation material；Second stage fermentation material is squeezed to the suspension being obtained by filtration, adjusts pH to 2.5-3.0, it is concentrated
The biochemical fulvic acid that concentration is 65% can be obtained in purification.
Example IV: the Optimization of preparation of fulvic acid chelated zinc bio-fertilizer
(1) condition optimizing is chelated
By biochemical yellow corruption 1kg, zinc sulfate 4kg, potassium dihydrogen phosphate 9kg are uniformly mixed with 55kg water, adjustment pH7.0 investment
Into first order reaction kettle, three-level is divided to carry out segmented chelatropic reaction, fulvic acid chelated zinc solution is obtained, when different experiments group chelates
Between chela temperature and chelating time parameter ginseng be shown in Table 5；Detect chelation percent in the fulvic acid chelated zinc solution of preparation.
Table 5: influence of the different chelation stage chelating conditions to fulvic acid chelated zinc
| First stage | Second stage | Phase III | Chelation percent (%) | |
| A group | 30 DEG C, 60min | 50 DEG C, 30min | 75 DEG C, 25min | 92.5 |
| B group | 30 DEG C, 50min | 50 DEG C, 25min | 75 DEG C, 20min | 89.5 |
| C group | 30 DEG C, 40min | 50 DEG C, 20min | 75 DEG C, 15min | 82.7 |
| D group | 35 DEG C, 60min | 60 DEG C, 30min | 80 DEG C, 25min | 93.2 |
| E group | 35 DEG C, 50min | 60 DEG C, 25min | 80 DEG C, 20min | 93.7 |
| F group | 35 DEG C, 40min | 60 DEG C, 20min | 80 DEG C, 15min | 87.4 |
| G group | 40 DEG C, 60min | 70 DEG C, 30min | 85 DEG C, 25min | 92.1 |
| H group | 40 DEG C, 50min | 70 DEG C, 25min | 85 DEG C, 20min | 90.3 |
| I group | 40 DEG C, 40min | 70 DEG C, 20min | 85 DEG C, 15min | 90.6 |
Chelates temperature and reaction time are an important factor for influencing chelation percent.It can be seen from result in table 5 when chelating
Between it is consistent when, as the temperature rises, chelation percent gradually increases, when the timing of chelates temperature one, as chelation percent is not with chela
It closes the growth of time and increases, it is evident that chelation percent is up in fulvic acid chelated zinc solution under the experiment condition of E group
93.7%, it is 93.2% although chelation percent is also higher under the conditions of D group, the chelating time is long, increases time cost.It is comprehensive
It closes and considers selection E group, i.e. 35 DEG C of first stage chelates temperature, chelate time 50min, 60 DEG C of second stage chelates temperature, chelate
Time 25min, chelates time 20min by 80 DEG C of phase III chelates temperature, is optimal chelating condition.
(2) pH condition optimizing is chelated
By biochemical yellow corruption 1kg, zinc sulfate 4kg, potassium dihydrogen phosphate 9kg are uniformly mixed with 55kg water, adjust pH, put into
In first order reaction kettle, divide three-level carry out segmented chelatropic reaction, 35 DEG C of first stage chelates temperature, chelate time 50min, second
60 DEG C of stage chelates temperature, time 25min is chelated, 80 DEG C of phase III chelates temperature, time 20min is chelated, obtains fulvic acid
Zinc solution is chelated, experiment each group is arranged different pH to being chelated, detects chelation percent in the fulvic acid chelated zinc solution of preparation.
Table 6: influence of the different chelation stage chelating conditions to fulvic acid chelated zinc
| PH value | Chelation percent (%) | |
| A group | 4.0 | 81.4 |
| B group | 5.0 | 90.7 |
| C group | 6.0 | 94.2 |
| D group | 7.0 | 93.9 |
| E group | 8.0 | 94.2 |
| F group | 9.0 | 94.4 |
The chelation percent that pH significantly affects fulvic acid chelated zinc solution is chelated it can be seen from result in table 6, when pH is lower than
When 6.0, chelation percent is lower, when pH noble quality 6.0, chelation percent increasing degree simultaneously obvious, kept stable, it is evident that
Under the experiment condition of C group, when pH value is 6.0, chelation percent relatively up to 94.2% in fulvic acid chelated zinc solution.Comprehensively consider choosing
C group condition is selected, i.e., is optimal chelating pH condition when chelating pH is 6.0.
(3) orthogonal optimization
On the basis of single factor experiment result, according to orthogonal experiment respectively according to biochemical fulvic acid: zinc sulfate: biphosphate
Potassium ratio (A), mix bacterium agent ratio (B), fulvic acid chelated zinc solution: mix bacterium agent (C), xanthan gum additive amount (D) and crust
Plain additive amount (E) obtains fulvic acid chelated zinc bio-fertilizer after preparation, after 1 times of dilution, the foliage-spray fertilizer is measured to walnut
The influence of Zn content in fruit carries out the horizontal experiment of five factor three according to orthogonal experiment Combination Design.The factor and level of test
Encoded radio ginseng is shown in Table 7.
Table 7: the factor and level code value of test
Orthogonal optimization difference preparation condition is to the influence test result of Zn content in Walnut Fruit and analysis referring to 8 institute of table
Show.
Table 8: orthogonal experiments and analysis
As can be seen from Table 8, in the range of experimental design, biochemical fulvic acid: zinc sulfate: potassium dihydrogen phosphate ratio is to core
The influence of Zn content is maximum in Peach fruits.Obtaining optimum optimizing condition in conjunction with table 7 and table 8 is A3B2C3D2E2, i.e., fulvic acid chelated
Raw material is according to biochemical fulvic acid in zinc solution preparation: zinc sulfate: potassium dihydrogen phosphate=1:5:10 mixing, mix bacterium agent is according to short and small
Bacillus: red pseudomonas: Methylotrophic bacillus: Avid kyowamycin: saccharomycete=4:5:5:2:4 is mixed with,
Fulvic acid chelated zinc solution: mix bacterium agent=7:1 ratio mixing, xanthan gum additive amount is 2% and chitin additive amount is
1.5%, the fulvic acid chelated zinc bio-fertilizer prepared, dilution 1 times after application with walnut blade face on and blade face under, the core of acquisition
Zn content highest in Peach fruits.
It is obtained using single factor test and orthogonal test, item is most preferably prepared using fulvic acid chelated zinc bio-fertilizer provided by the invention
Part are as follows: according to parts by weight than meter, by 1 part of biochemical fulvic acid, 5 parts of zinc sulfate, 10 parts of potassium dihydrogen phosphate, 50 parts of water mixing are equal
It is even, it puts into first order reaction kettle, adjusts pH 6.0, reaction temperature is 35 DEG C, and reaction time 50min, first order reaction terminates
After be transferred in second order reaction kettle, reaction temperature is 60 DEG C, reaction time 25min, is transferred to third-order reaction after second order reaction
In kettle, reaction temperature is 80 DEG C, reaction time 20min, by the chelatropic reaction of third-order reaction kettle, obtains fulvic acid chelated zinc
Solution；Mix bacterium agent is according to bacillus pumilus: red pseudomonas: Methylotrophic bacillus: Avid kyowamycin: saccharomycete
=4:5:5:2:4, is mixed, and mix bacterium agent is prepared, and detection viable count reaches 8.6 × 1010cfu/ml；By fulvic acid
7 parts and 1 part of mix bacterium agent of zinc solution are chelated, after mixing, fertilizer of chelated zinc mixed liquor is made, by weight percentage, to chela
It closes in zinc fertilizer mixed liquor and 2% xanthan gum, 1.5% chitin is added, play thallus suspension and water retention, be mixed evenly,
Fulvic acid chelated zinc bio-fertilizer is prepared, after 1 times of dilution, application is with behind walnut blade face, and zinc contains in the Walnut Fruit of acquisition
Amount is up to 2.95mg/kg.
Example IV: fulvic acid chelated zinc bio-fertilizer is applied in walnut plantation
Fulvic acid chelated zinc bio-fertilizer is prepared by using the method for embodiment one, embodiment two, and in Wensu core
Peach forest farm is tested, and by the way that 7 test groups are arranged, it is five kinds that the application, which is prepared fulvic acid chelated zinc bio-fertilizer and is diluted with water,
The fertilizer of various concentration, walnut bears fruit-and fruit expanding period carries out zinc fertilizer spraying test, and it is positive and negative in blade through the uniform spraying of mist
On face, it is diluted liquid for 15 kilograms of dosage per acre.Phase spray bear fruit for the first time, sprays primary, even spray 3 times again after 15 days.One
As selection sprayed before calm 10 points of the morning of fine day and after 7 points of afternoon, filter out economical, efficient, energy-efficient yellow corruption
Sour chelated zinc bio-fertilizer method of administration.
Table 9: the influence after different dilution chelating fertilizer applications to Zn content in Walnut Fruit
By carrying out fulvic acid chelated zinc bio-fertilizer spraying test to walnut phase of bearing fruit, as a result ginseng is shown in Table 9, pass through by
Fulvic acid chelated zinc bio-fertilizer, which is diluted, to be sprayed, it can be seen that fulvic acid chelated zinc bio-fertilizer dilutes 8 times and 10 times of sprays of dilution
Shi Hou, Zn content difference is little in Walnut Fruit, but when extension rate is greater than 10 times, Zn content is substantially reduced in Walnut Fruit,
Fulvic acid chelated zinc bio-fertilizer dilutes 10 times, and being equivalent to 1.5kg/ mus and spraying Zn content in rear Walnut Fruit is 2.96mg/kg, with
Control is compared, and Zn content improves 44.6% in Walnut Fruit, and compared with commercially available soil fertilization material, Zn content is improved in Walnut Fruit
37.5%, with it is commercially available spray foliar fertilizer compared with, in Walnut Fruit Zn content improve 28.7%.
It carries out spraying examination by fulvic acid chelated zinc bio-fertilizer prepared by the optimum ratio in embodiment one, embodiment two
It tests, preparation condition are as follows: biochemical fulvic acid, according to parts by weight than meter, by 12 parts of stalk, 5 parts of wheat bran, 3 parts of sawdust, green peel of walnut
3 parts, 1 part of thin skin walnut shell, 1 part of urea are uniformly mixed, Mechanical Crushing, rear that 35 parts of clear water mixing are added, and obtain mixed raw material,
5% first stage fermentation mix bacterium agent is accessed, the first stage ferments mix bacterium agent according to volume basis, chain acidophil: short and small bud
Spore bacillus: whiterot fungi: Clostridium thermocellum=1:2:1:1, after being fermented, access 5% second stage fermentation mix bacterium agent, second
Stage fermentation mix bacterium agent is according to volume basis, and bacillus subtilis: aspergillus niger: Trichoderma=2:2:1, fermentation obtain biochemical yellow
Rotten acid；According to parts by weight than meter, by 1 part of biochemical fulvic acid, 5 parts of zinc sulfate, 10 parts of potassium dihydrogen phosphate, 60 parts of water mixing are equal
It is even, it puts into first order reaction kettle, adjusts pH 6.0, reaction temperature is 35 DEG C, and reaction time 50min, first order reaction terminates
After be transferred in second order reaction kettle, reaction temperature is 60 DEG C, reaction time 25min, is transferred to third-order reaction after second order reaction
In kettle, reaction temperature is 80 DEG C, reaction time 20min, by the chelatropic reaction of third-order reaction kettle, obtains fulvic acid chelated zinc
Solution；According to volume basis, bacillus pumilus: red pseudomonas: Methylotrophic bacillus: Avid kyowamycin is selected:
Saccharomycete=4:5:5:2:4, is mixed, and mix bacterium agent is prepared, and detection viable count reaches 8.6 × 1010cfu/ml；According to
By 7 parts and 1 part of mix bacterium agent of fulvic acid chelated zinc solution after mixing, fertilizer of chelated zinc mixing is made in weight fraction ratio meter
2% xanthan gum, 1.5% chitin are added into fertilizer of chelated zinc mixed liquor by weight percentage for liquid, play thallus suspend and
Water retention is mixed evenly, and prepares fulvic acid chelated zinc bio-fertilizer, carries out walnut leaf surface spraying test, according to
After 1.5kg/ mus spray, Zn content is higher than 2.96mg/kg in Walnut Fruit, and Zn content is higher in walnut kernel, fulvic acid chela
The fertilization effect for closing zinc bio-fertilizer is more preferable, realizes that walnut zinc-rich promotes the purpose of health-care efficacy.
By it is a series of screening and compliance test result test show the present invention by using mix bacterium agent with it is fulvic acid chelated
Zinc solution, which blends, prepares fulvic acid chelated zinc bio-fertilizer, and fulvic acid chelated zinc bio-fertilizer microorganism after walnut foliage applying is fast
Fast mass propagation, can degrading pesticide fertilizer residue, eliminate heavy metal pollution, fulvic acid is utilized in fulvic acid chelated zinc bio-fertilizer
Strong complexing power and Zinc Ions Chelated, be more advantageous to the absorption of Walnut, improve the utilization rate of zinc fertilizer, improve walnut product
Quality reduces pesticide residue, and Zn content is higher in walnut kernel, realizes that walnut zinc-rich promotes the purpose of health-care efficacy.
The above embodiment is merely an example for clearly illustrating the present invention, and does not limit the embodiments.
For those of ordinary skill in the art, other various forms of variations can also be made on the basis of the above description
Or it changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation thus extended
Or it changes and is still in the protection scope of this invention.
Claims (10)
1. a kind of preparation method of fulvic acid chelated zinc bio-fertilizer, which is characterized in that method acquisition is prepared by the following procedure:
(1) fulvic acid chelated zinc solution preparation: according to parts by weight than meter, by 1-2 parts of biochemical fulvic acid, 4-6 parts of zinc sulfate, phosphorus
9-11 parts of acid dihydride potassium, 45-55 parts of water are uniformly mixed, and put into first order reaction kettle, adjust pH6.0-7.0, and reaction temperature is
It 30-40 DEG C, reaction time 40-60min, being transferred in second order reaction kettle after first order reaction, reaction temperature is 50-70 DEG C,
Reaction time is 20-30min, is transferred in third-order reaction kettle after second order reaction, and reaction temperature is 75-85 DEG C, the reaction time
Fulvic acid chelated zinc solution is obtained by the chelatropic reaction of third-order reaction kettle for 15-25min；
(2) prepared by mix bacterium agent: selecting bacillus pumilus, Methylotrophic bacillus, red pseudomonas, Avid kyowamycin
It is mixed with saccharomycete, prepares mix bacterium agent, detection viable bacteria reaches 8.6 × 1010cfu/ml；
(3) preparation of fulvic acid chelated zinc bio-fertilizer: according to parts by weight than meter, fulvic acid chelated zinc prepared by step (1)
6-8 parts of 1-2 parts of mix bacterium agent prepared with step (2) of solution, after mixing, obtained fertilizer of chelated zinc mixed liquor, by weight hundred
Divide than meter, 1-3% xanthan gum, 1-2% chitin are added into fertilizer of chelated zinc mixed liquor, plays thallus suspension and water retention, it is mixed
Conjunction stirs evenly, and prepares fulvic acid chelated zinc bio-fertilizer.
2. a kind of preparation method of fulvic acid chelated zinc bio-fertilizer as described in claim 1, which is characterized in that the fulvic acid chela
Zinc solution preparation is closed, according to parts by weight than meter, by 1 part of biochemical fulvic acid, 5 parts of zinc sulfate, 10 parts of potassium dihydrogen phosphate, 60 parts of water
It is uniformly mixed, puts into first order reaction kettle, adjust pH 6.0, reaction temperature is 35 DEG C, reaction time 50min, and level-one is anti-
It is transferred to after answering in second order reaction kettle, reaction temperature is 60 DEG C, reaction time 25min, is transferred to three after second order reaction
In order reaction kettle, reaction temperature is 80 DEG C, reaction time 20min, by the chelatropic reaction of third-order reaction kettle, obtains fulvic acid
Chelate zinc solution.
3. a kind of preparation method of fulvic acid chelated zinc bio-fertilizer as described in claim 1, which is characterized in that the mix bacterium agent
Preparation selects bacillus pumilus: red pseudomonas: Methylotrophic bacillus: Avid kyowamycin according to volume basis:
Saccharomycete=4:5:5:2:4, is mixed, and mix bacterium agent is prepared, and detection viable bacteria reaches 8.6 × 1010cfu/g。
4. a kind of preparation method of fulvic acid chelated zinc bio-fertilizer as described in claim 1, which is characterized in that according to parts by weight
Than meter, by 7 parts and 1 part of mix bacterium agent of fulvic acid chelated zinc solution, after mixing, fertilizer of chelated zinc mixed liquor is made, by weight
2% xanthan gum, 1.5% chitin are added into fertilizer of chelated zinc mixed liquor for percentages, play thallus suspension and water retention, mix
Conjunction stirs evenly, and prepares fulvic acid chelated zinc bio-fertilizer.
5. a kind of preparation method of the biochemical fulvic acid in fulvic acid chelated zinc bio-fertilizer, which is characterized in that be prepared by the following procedure
Method obtains:
(1) feedstock processing: according to parts by weight than meter, by 11-13 parts of stalk, 4-6 parts of wheat bran, 2-4 parts of sawdust, green peel of walnut 2-
4 parts, 1-2 parts of thin skin walnut shell, 1-2 parts of urea are uniformly mixed, Mechanical Crushing, rear that clear water 30-40 parts of mixing is added, and are mixed
Close raw material；
(2) first stage ferments: by weight percentage, in the mixed raw material obtained to step (1), first stage fermentation is added
Mix bacterium agent 5% maintains 48h, during which turns 2 times when material temperature rises to 65 DEG C or more, obtains first stage fermentation material；
(3) second stage is fermented: by weight percentage, in the first stage fermentation material obtained to step (2), step is added
(1) addition mixed raw material 20% and second stage the fermentation mix bacterium agent 5% prepared, is uniformly mixed fermentation 96h, during which turns 4
It is secondary, obtain second stage fermentation material；
(4) the second stage fermentation material of step (3) preparation the preparation of biochemical fulvic acid: is squeezed into the suspension being obtained by filtration, warp
The biochemical fulvic acid that concentration is 65% can be obtained in concentration purification.
6. a kind of preparation method of the biochemical fulvic acid in fulvic acid chelated zinc bio-fertilizer, feature exist as claimed in claim 5
In, the biochemical fulvic acid, first stage fermentation mix bacterium agent, according to volume basis, chain acidophil: bacillus pumilus: white
Rotten bacterium: Clostridium thermocellum=1:2:1:1.
7. a kind of preparation method of the biochemical fulvic acid in fulvic acid chelated zinc bio-fertilizer, feature exist as claimed in claim 5
In, the biochemical fulvic acid, second stage fermentation mix bacterium agent, according to volume basis, bacillus subtilis: aspergillus niger: trichoderma
Bacterium=2:2:1.
8. a kind of preparation method of the biochemical fulvic acid in fulvic acid chelated zinc bio-fertilizer, feature exist as claimed in claim 5
In the biochemical fulvic acid raw material, according to parts by weight than meter, by 12 parts of stalk, 5 parts of wheat bran, 3 parts of sawdust, green peel of walnut 3
Part, 1 part of thin skin walnut shell, 1 part of urea are uniformly mixed, Mechanical Crushing, rear that 35 parts of clear water mixing are added, and obtain mixed raw material.
9. a kind of fulvic acid chelated zinc bio-fertilizer obtained such as claim 1 or 5 any one preparation methods.
10. application of the fulvic acid chelated zinc bio-fertilizer as claimed in claim 9 in walnut plantation.
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