CN109984148A - A kind of composition and application containing chitosan and S- abscisic acid - Google Patents
A kind of composition and application containing chitosan and S- abscisic acid Download PDFInfo
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- CN109984148A CN109984148A CN201711494996.6A CN201711494996A CN109984148A CN 109984148 A CN109984148 A CN 109984148A CN 201711494996 A CN201711494996 A CN 201711494996A CN 109984148 A CN109984148 A CN 109984148A
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- chitosan
- abscisic acid
- cold
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 69
- 239000000203 mixture Substances 0.000 title claims abstract description 65
- 239000005987 S-Abscisic acid Substances 0.000 title claims abstract description 59
- 230000002595 cold damage Effects 0.000 claims abstract description 20
- 238000005507 spraying Methods 0.000 claims abstract description 18
- 239000004530 micro-emulsion Substances 0.000 claims abstract description 15
- -1 aqua Substances 0.000 claims abstract description 3
- 239000000375 suspending agent Substances 0.000 claims abstract description 3
- 239000004562 water dispersible granule Substances 0.000 claims abstract description 3
- 239000004563 wettable powder Substances 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- ORVGYTXFUWTWDM-UHFFFAOYSA-N silicic acid;sodium Chemical compound [Na].O[Si](O)(O)O ORVGYTXFUWTWDM-UHFFFAOYSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000008267 milk Substances 0.000 claims 1
- 210000004080 milk Anatomy 0.000 claims 1
- 235000013336 milk Nutrition 0.000 claims 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 claims 1
- 235000012239 silicon dioxide Nutrition 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 239000004115 Sodium Silicate Substances 0.000 abstract description 13
- 235000019795 sodium metasilicate Nutrition 0.000 abstract description 13
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 abstract description 13
- 229910052911 sodium silicate Inorganic materials 0.000 abstract description 13
- 230000008635 plant growth Effects 0.000 abstract description 3
- 239000000839 emulsion Substances 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 20
- 238000012360 testing method Methods 0.000 description 20
- 230000000694 effects Effects 0.000 description 16
- 229920002101 Chitin Polymers 0.000 description 11
- 238000012216 screening Methods 0.000 description 7
- 230000012010 growth Effects 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
- 241001582888 Lobus Species 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000017060 Arachis glabrata Nutrition 0.000 description 3
- 244000105624 Arachis hypogaea Species 0.000 description 3
- 235000010777 Arachis hypogaea Nutrition 0.000 description 3
- 235000018262 Arachis monticola Nutrition 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 229930002875 chlorophyll Natural products 0.000 description 3
- 235000019804 chlorophyll Nutrition 0.000 description 3
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 238000000407 epitaxy Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000020232 peanut Nutrition 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 229920001410 Microfiber Polymers 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000003658 microfiber Substances 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000012372 quality testing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
- A01N25/06—Aerosols
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Dispersion Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to plant growth regulating fields, it is related to a kind of agriculturally useful compositions and purposes containing chitosan Yu S- abscisic acid, contain 5-40% sodium metasilicate in composition, the mass parts ratio of chitosan and S- abscisic acid is 1:1-100:1, the gross mass that the quality summation of chitosan and S- abscisic acid accounts for composition is 5-80%, and microemulsion, aqua, suspending agent, aqueous emulsion, wettable powder, water dispersible granules are made in composition.Composition 250-1000 times is diluted before cold damage arrives to apply by spraying.
Description
Technical field
The invention belongs to plant growth regulating field, be related to a kind of composition containing chitosan and S- abscisic acid and its
Application in terms of cold-resistant.
Background technique
In recent years, extended this harsh fact for being unfavorable for plant growth in face of weather mutation, winter low temperature, in life
The counter-measure based on defending should be carried out in production, occurs the Plant antifreezer being widely used on crops on the market, swashed
The plain class antifreezing agent mechanism of action is relatively simple, and antifreezing effect is unstable, and the cold-resistance agent for exploring novel effect stability is extremely urgent.
It is estimated that the chitin (chitin, chitin, chitin) generated in nature is the reserves for being only second to cellulose
Maximum biopolymer, and it has functional character more abundant than fiber rope, it be widely present in fungal cell wall,
In the outer skeleton of nematode microfibre sheath, arthropod and many insect gut inner walls.1811, French scientist mentioned from mushroom
A kind of substance of like fibrous element has been got, and has been named as chitin, chitin has been put in later after being heated in NaOH, is obtained
A kind of novel substance chitosan is arrived.Chitosan (chitosan), the Portugal chemical name (Isosorbide-5-Nitrae) -2- amino -2- deoxidation-β-D-
Glycan is the water-soluble products that chitin generates after strong lye deacetylate, and relative molecular mass is usually in 5000 dongles
Or more, some is up to 1,000,000 dalton or more.Due to the poor dissolubility of chitin, its application is limited, and chitin
Not only dissolubility enhances glycan, but also nontoxic and have good biocompatibility, degradable, and application range is wider.
S- abscisic acid is also the growth balance factor of plant, is balance plant endogenous hormones and related growth activity substance
The key factor of metabolism has and promotes plant Balance Absorption water, fertilizer and coordinate the ability that is metabolized in vivo, can Effective Regulation plant
Root, hat and nutrient growth and reproductive growth play a significant role the quality, the yield that improve crops.Also as the anti-of plant
Inverse inducible factor, can degeneration-resistant immune system effectively in activated plant body.It is consolidated with training source, enhances the energy of comprehensive plant resistance
Power, the recovery for protecting production and ecological environment to mitigation in agricultural production play a significant role.
Chitosan of the present invention using deacetylation higher than 80% is anti-through specificity enzyme hydrolysis, control after swelling as raw material
The processes such as terminal, hydrolyzate concentration, freeze-drying are answered to prepare the water-soluble chitosan of specified molecular weight.Inventor carries out
The research of the composition of a large amount of chitosan and S- abscisic acid, it was found that it is a kind of containing chitosan and S- abscisic acid have it is bright
The composition of aobvious cold-resistant effect.
Summary of the invention
The present invention provides a kind of composition containing chitosan and S- abscisic acid and its in the purposes of cold-resistant.
The present invention provides a kind of agriculturally useful compositions containing chitosan Yu S- abscisic acid, which is characterized in that in composition
Containing 5-40% sodium metasilicate, the relative molecular mass of chitosan is 5000 dalton~20000 dalton, chitosan and S-
The mass parts ratio of abscisic acid is 1:1-100:1.
Preferably, silicic acid sodium content is 10%, and the mass parts ratio of chitosan and S- abscisic acid is 10:1-50:1.
In combinations of the above object, the mass parts ratio of chitosan and S- abscisic acid is 39:1
The gross mass that the quality summation of composition of the invention, chitosan and S- abscisic acid accounts for composition is 5-80%,
Preferably, it is 20-50% that the quality summation of chitosan and S- abscisic acid, which accounts for the gross mass of composition,.
Above-mentioned composition of the present invention can be made into microemulsion, aqua, suspending agent, aqueous emulsion, wettable powder, water dispersible granules
Deng.Preferably, microemulsion is made in composition.
The present invention provides above-mentioned compositions to the purposes in terms of plant cold resistance.
Composition is diluted 250-1000 times before cold damage arrives and applied by spraying by the purposes of above-mentioned composition of the present invention.It is excellent
Choosing, the spraying application in 1-3 days before cold damage arrives.
The composition of invention compared with prior art, generate it is following the utility model has the advantages that
(1) composition quality of the invention is stablized.
(2) composition has positive effect to the cold-resistant of plant.
Specific embodiment
Below with reference to embodiment, the invention will be further described, but the present invention is never limited to following embodiments.Select phase
To the chitosan in molecular mass different range section (when 5000 dalton~20000 dalton, 30000 dalton~
When 100000 dalton), preliminary test discovery, the relative molecular mass of chitosan is 5000 dalton~20000 dalton
When, the cold-resistant effect of chitosan and S- abscisic acid composition is preferable.In following embodiment, the relative molecular mass of chitosan
For 5000 dalton~20000 dalton.
The composition of the different ratio of 1 chitosan of embodiment and S- abscisic acid tests the cold-resistant of crop
The composite microemulsion containing chitosan and S- abscisic acid of different ratio is prepared (in the composition of different ratio
The kind and content of auxiliary agent are all the same).Cold-resistant test is carried out to potted plant crop with the composition of different ratio, and sets corresponding list
Agent and blank control, each processing dilute 500 times to peanut, long bean and the seedling of cucumber spraying primary (seedling equal 4-6 leaf phase),
Every kind of crop is 20 plants or more spraying, and spraying treatment is put into 4 DEG C of insulating boxs and carries out low-temperature epitaxy after 24 hours, takes out and puts after 34 hours
The investigation of cold damage result is carried out under room temperature (25 DEG C or so).Crop leaf cold damage grade scale and calculation formula are as follows, as a result see
Table 1.
0 grade: without cold damage symptom
1 grade: the slight shrinkage of blade, the slightly dehydration of one third blade, lobus cardiacus are normal
2 grades: leaf-shrinkage, one third leaves water loss, lobus cardiacus are normal
3 grades: leaf-shrinkage, 2/3rds leaves water loss, lobus cardiacus are normal
4 grades: whole strain leaves water loss is wilted, and lobus cardiacus has slight dehydration, can restore under room temperature
5 grades: the whole serious dehydration of strain blade is wilted, and cannot be restored under room temperature
The composition of 1 chitosan of table and S- abscisic acid different ratio tests crop cold resistance
The composition of chitosan and S- abscisic acid different ratio is shown in Table 1 to crop cold resistance test result.As a result, it has been found that
To in the effect of 3 kinds of crops, chitosan lures anti-the composition of S- abscisic acid containing 4% chitosan and different content with S-
The composition of plain different ratio is below single dose to crop cold damage index and compares, and the proportion of chitosan and S- abscisic acid is 40:1
The cold-resistant effect of relatively other 2 proportions of Shi Kanghan effect is obvious.Composition of the embodiment 2 containing chitosan and S- abscisic acid
Builder screening
1, the preparation of the composition containing different builders
The preparation of the 4.0% chitosan S- abscisic acid microemulsion containing 10% sodium metasilicate.3.9 grams of chitins of precise are poly-
Sugar is sufficiently dissolved with 6 grams of lactic acid, and 0.1 gram of S- abscisic acid is added, is sufficiently mixed, and sodium metasilicate and each 10 grams of Tween 80 are added, will mix
Object is closed stir in blender, water complements to 100g, both quality Jing Guo quality testing it is stable contain 10% sodium metasilicate
4.0% chitosan S- abscisic acid microemulsion.
Same method is prepared, containing 0.5% salicylic 4.0% chitosan S- abscisic acid (3.9% chitosan+
0.1%S- abscisic acid) microemulsion and 10% chelated calcium 4.0% chitosan S- abscisic acid (3.9% chitosan+0.1%
S- abscisic acid) microemulsion.
2, the synergy of 4.0% chitosan S- abscisic acid (3.9% chitosan+0.1%S- abscisic acid) microemulsion helps
Agent screening
4.0% chitosan S- abscisic acid of composition (3.9% chitosan+0.1%S- abscisic acid) micro emulsion is carried out
The builder screening test of agent.If corresponding single dose and blank control, with 4.0% chitosan containing different builders
500 times of composition dilution of S- abscisic acid are spraying to corn, peanut, long bean and the seedling of cucumber once (seedling equal 4-6 leaf phase),
Every kind of crop is 20 plants or more spraying, is put within 24 hours after spraying treatment 4 DEG C of insulating boxs and carries out low-temperature epitaxy, takes out and put after 24 hours
The investigation of cold damage result, crop leaf cold damage grade scale and calculation formula such as embodiment 1, knot are carried out under room temperature (25 DEG C or so)
Fruit is shown in Table 2.
The builder screening test of 2 4.0% chitosan S- abscisic acid of table
The builder screening test of 4.0% chitosan S- abscisic acid the results are shown in Table 2.As a result, it has been found that 4 to test
In kind crop, the composition of the 4.0% chitosan S- abscisic acid containing different synergist is below the cold damage index of crop
4.0% chitosan S- abscisic acid and single dose control, and with the 4.0% chitosan S- abscisic acid containing 10% sodium metasilicate
The cold-resistant effect of the relatively other processing of cold-resistant effect is obvious, the group containing 10% chelated calcium 4.0% chitosan S- abscisic acid
The cold-resistant effect for closing object is taken second place.
Micro-emulsion composition of the embodiment 3 containing 10% sodium metasilicate further tests plant cold resistance
The composition of the different ratio containing 10% sodium metasilicate and chitosan and S- abscisic acid is further tested to plant
Cold damage test: carrying out cold-resistant test to potted plant crop with composition, and set corresponding single dose and blank control, right with 500 times
Crop seedling is spraying primary (seedling equal 4-6 leaf phase), and every kind of crop is 20 plants or more spraying, spraying treatment before low-temperature treatment, low temperature
It is taken out after processing a period of time and is put in progress cold damage result investigation under room temperature (25 DEG C or so), test the plant height incrementss of corn
(cm) and average relative chlorophyll content (SPAD), as a result crop leaf cold damage grade scale and calculation formula such as embodiment 1 are shown in
Table 3.
Further the composition of test chitosan and S- abscisic acid different ratio tests crop cold resistance to table 3
The composition for testing the chitosan and S- abscisic acid of the different ratio containing 10% sodium metasilicate is further to crop
Cold-resistant test, chitosan and 3 different proportions of S- abscisic acid are 29:1,39:1,49:1, the results are shown in Table 3.As a result it sends out
Existing, in several crop seedlings of test, the composition containing chitosan and S- abscisic acid different ratio increases the plant height of corn
Dosage is compareed lower than comparison medicament and clear water, but relative chlorophyll content is above single dose control, during illustrating cold damage, contains chitin
The excessive growth that the composition of glycan and S- abscisic acid not will cause crop promotes crop to keep out the cold, but promote the content of crop chlorophyll
It improves；Single dose control is below to peanut, long bean and the cold damage of cucumber index, and the proportion of chitosan and S- abscisic acid is
The cold-resistant effect of the relatively other proportion 29:1 and 49:1 of cold-resistant effect is obvious when 39:1.
The screening using concentration of 4.0% chitosan S- abscisic acid microemulsion of the embodiment 4 containing synergist
Having carried out the 4.0% chitosan S- abscisic acid containing 10% sodium metasilicate, (3.9% chitosan+0.1%S- lures anti-
Element) microemulsion the screening using concentration.+ 10% sodium metasilicate of 4.0% chitosan S- abscisic acid is diluted into different concentration,
Primary (seedling equal 4-6 leaf phase) spraying to eggplant, tomato, capsicum and muskmelon seedling, every kind of crop is 10 plants or more spraying, spraying to locate
It is put within 48 hours after reason 4 DEG C of insulating boxs and carries out low-temperature epitaxy, taking-up, which is put under room temperature (25 DEG C or so), after 36 hours carries out cold damage
As a result it investigates, crop leaf cold damage grade scale and calculation formula such as embodiment 1 the results are shown in Table 4.
The composition of+10% sodium metasilicate of 4.0% chitosan S- abscisic acid of various concentration of the table 4 containing synergist is to work
The test of object cold-resistant
The composition of+10% sodium metasilicate of 4.0% chitosan S- abscisic acid of various concentration is tested to crop cold resistance
Effect the results are shown in Table 4.As a result, it has been found that after composition dilutes 250-2000 times, composition is to 4 kinds in 4 kinds of crops of test
Crop has apparent cold-resistant effect；It is preferable to dilute 250-1000 times of effect, restore being placed at room temperature for 72 hours ensuing crops
Normal growth.In practical application, recommend before cold damage arrives 1-3 days, composition dilution is used for 250-1000 times by spraying, if
Temperature is lower than 4 DEG C and low temperature is more than 2 days, recommends composition to dilute 250-500 times and applies.
Claims (10)
1. a kind of agriculturally useful compositions containing chitosan Yu S- abscisic acid, which is characterized in that contain 5-40% silicic acid in composition
Sodium, the relative molecular mass of chitosan are 5000 dalton~20000 dalton, the mass parts of chitosan and S- abscisic acid
Number is than being 1:1-100:1.
2. composition according to claim 1, which is characterized in that silicic acid sodium content is 10%, chitosan and S- abscisic acid
Mass parts ratio is 10:1-50:1.
3. composition according to claim 2, which is characterized in that the mass parts ratio of chitosan and S- abscisic acid is 39:1.
4. composition according to claim 3, which is characterized in that the quality summation of chitosan and S- abscisic acid accounts for composition
Gross mass is 5-80%.
5. composition according to claim 4, which is characterized in that the quality summation of chitosan and S- abscisic acid accounts for composition
Gross mass is 20-50%.
6. according to the composition of claim 4 or 5, which is characterized in that microemulsion, aqua, suspending agent, water and milk is made in composition
Agent, wettable powder, water dispersible granules.
7. composition according to claim 6, which is characterized in that microemulsion is made in composition.
8. purposes of the composition of claim 7 in terms of plant cold resistance.
9. purposes according to claim 8, which is characterized in that composition is diluted 250-1000 times before cold damage arrives and is answered by spraying
With.
10. purposes according to claim 9, which is characterized in that 250-500 times of dilution in 1-3 days is applied by spraying before cold damage arrives.
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Cited By (1)
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| CN114982761A (en) * | 2022-06-13 | 2022-09-02 | 西北农林科技大学 | Application of streptavadin dextran in preparation of plant immunity inducer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114982761A (en) * | 2022-06-13 | 2022-09-02 | 西北农林科技大学 | Application of streptavadin dextran in preparation of plant immunity inducer |
| CN114982761B (en) * | 2022-06-13 | 2024-03-08 | 西北农林科技大学 | Application of streptavidin-free glucan in preparation of plant immunity elicitor |
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| CN109984148B (en) | 2021-06-11 |
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Denomination of invention: A composition containing chitosan and s-inducer and its application Effective date of registration: 20220304 Granted publication date: 20210611 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2019460000007 |
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Address after: Zhengye Square, No. 7 Jinlian Road, Longhua District, Hainan Province, 571923 Patentee after: Hainan Zhengye Biotechnology Co.,Ltd. Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |