CN109984148B - Composition containing chitosan and S-abscisic acid and application thereof - Google Patents
Composition containing chitosan and S-abscisic acid and application thereof Download PDFInfo
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- 239000000203 mixture Substances 0.000 title claims abstract description 69
- 239000005987 S-Abscisic acid Substances 0.000 title claims abstract description 62
- 229920001661 Chitosan Polymers 0.000 title claims abstract description 57
- 230000002595 cold damage Effects 0.000 claims abstract description 20
- 239000004115 Sodium Silicate Substances 0.000 claims abstract description 16
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052911 sodium silicate Inorganic materials 0.000 claims abstract description 16
- 239000004530 micro-emulsion Substances 0.000 claims abstract description 15
- 239000000839 emulsion Substances 0.000 claims abstract description 3
- 239000004562 water dispersible granule Substances 0.000 claims abstract description 3
- 239000004563 wettable powder Substances 0.000 claims abstract description 3
- 238000005507 spraying Methods 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 208000009084 Cold Injury Diseases 0.000 abstract description 5
- 230000008635 plant growth Effects 0.000 abstract description 3
- 230000033228 biological regulation Effects 0.000 abstract description 2
- 239000000375 suspending agent Substances 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 23
- 230000000694 effects Effects 0.000 description 13
- 238000012360 testing method Methods 0.000 description 12
- 230000012010 growth Effects 0.000 description 9
- 238000012216 screening Methods 0.000 description 7
- 238000011282 treatment Methods 0.000 description 6
- 229920002101 Chitin Polymers 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000011835 investigation Methods 0.000 description 4
- 244000105624 Arachis hypogaea Species 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 229930002875 chlorophyll Natural products 0.000 description 3
- 235000019804 chlorophyll Nutrition 0.000 description 3
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 235000020232 peanut Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 230000002528 anti-freeze Effects 0.000 description 2
- 230000006196 deacetylation Effects 0.000 description 2
- 238000003381 deacetylation reaction Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
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- 229920002498 Beta-glucan Polymers 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000000105 evaporative light scattering detection Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000013383 initial experiment Methods 0.000 description 1
- 235000021332 kidney beans Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000001724 microfibril Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
- A01N25/06—Aerosols
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of plant growth regulation, and relates to an agricultural composition containing chitosan and S-abscisic acid and application thereof, wherein the composition contains 5-40% of sodium silicate, the mass part ratio of the chitosan to the S-abscisic acid is 1:1-100:1, the mass sum of the chitosan and the S-abscisic acid accounts for 5-80% of the total mass of the composition, and the composition is prepared into microemulsion, water aqua, suspending agent, aqueous emulsion, wettable powder and water dispersible granules. The composition is diluted by 250 times and sprayed by 1000 times before cold injury comes.
Description
Technical Field
The invention belongs to the field of plant growth regulation, and relates to a composition containing chitosan and S-abscisic acid and application thereof in cold resistance.
Background
In recent years, in the face of the severe fact that the growth of crops is not facilitated due to climate change and low temperature in winter, a countermeasure mainly aiming at defense is made in production, plant antifreezes widely used on crops appear on the market, hormone antifreezes are single in action mechanism and unstable in antifreezing effect, and the search for novel cold-resistant agents with stable effects is urgent.
Chitin (chitin ) produced in nature is estimated to be the next largest biopolymer to cellulose, and it has functional properties richer than the fiber cord, and it is found widely in fungal cell walls, nematode microfibril sheaths, arthropod exoskeleton and many insect gut inner walls. In 1811, scientists in France extracted a cellulose-like substance from mushrooms and named it as chitin, and later heated it in NaOH to obtain a new substance, chitosan. Chitosan (chitosan), chemical name (1, 4) -2-amino-2-deoxy- β -D-glucan, is a water-soluble product produced by deacetylation of chitin with concentrated aqueous alkali, and has a relative molecular mass of usually more than 5000 daltons, and some up to more than 100 kilodaltons. Chitin is limited in its application due to its relatively poor solubility, and chitosan has not only enhanced solubility, but also non-toxicity, good biocompatibility, easy degradation, and a wider application range.
The S-abscisic acid, also called growth balance factor of plant, is a key factor for balancing endogenous hormone of plant and metabolism of related growth active substances, has the capability of promoting plant to absorb water and fertilizer in balance and coordinating metabolism in vivo, can effectively regulate and control root and crown of plant and vegetative growth and reproductive growth, and has important effect on improving quality and yield of crops. Also can be used as stress-resistant induction factor of plants, and can effectively activate the stress-resistant immune system in plants. Has the capability of cultivating sources and consolidating the foundation and enhancing the comprehensive resistance of plants, and plays an important role in reducing disasters and protecting the yield and restoring the ecological environment in agricultural production.
The present invention prepares water soluble chitosan with specific molecular weight with chitosan of deacetylation degree higher than 80% as material and through specific enzyme hydrolysis, reaction end point control, hydrolysate concentration, freeze drying and other steps. The inventors have conducted extensive studies on a composition of chitosan and S-abscisic acid, and have found a composition containing chitosan and S-abscisic acid and having a remarkable cold-resistant effect.
Disclosure of Invention
The invention provides a composition containing chitosan and S-abscisic acid and application thereof in cold resistance.
The invention provides an agricultural composition containing chitosan and S-abscisic acid, which is characterized in that the composition contains 5-40% of sodium silicate, the relative molecular mass of the chitosan is 5000-20000 daltons, and the mass part ratio of the chitosan to the S-abscisic acid is 1:1-100: 1.
Preferably, the content of the sodium silicate is 10%, and the mass part ratio of the chitosan to the S-abscisic acid is 10:1-50: 1.
In the composition, the mass part ratio of the chitosan to the S-abscisic acid is 39:1
In the composition of the invention, the total mass of the chitosan and the S-abscisic acid accounts for 5-80% of the total mass of the composition, and preferably, the total mass of the chitosan and the S-abscisic acid accounts for 20-50% of the total mass of the composition.
The composition can be prepared into microemulsion, aqueous solution, suspending agent, aqueous emulsion, wettable powder, water dispersible granules and the like. Preferably, the composition is formulated as a microemulsion.
The invention provides the application of the composition in the cold resistance of plants.
The application of the composition disclosed by the invention is to dilute the composition by 250-fold and 1000-fold spray application before cold injury comes. Preferably, the spray is applied 1-3 days before cold injury.
Compared with the prior art, the composition of the invention has the following beneficial effects:
(1) the composition of the invention has stable quality.
(2) The composition has obvious effect on cold resistance of plants.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The present invention will be further illustrated with reference to the following examples, but the present invention is by no means limited to the following examples. Initial experiments show that the cold-resistant effect of the chitosan and S-abscisic acid composition is better when the relative molecular mass of the chitosan is 5000-20000 daltons and 30000-100000 daltons in different ranges of relative molecular mass. In the following examples, the relative molecular mass of chitosan is 5000 to 20000 daltons.
Example 1 Cold resistance test of different ratios of Chitosan to S-abscisic acid on crops
Preparing the composition microemulsion containing chitosan and S-abscisic acid with different proportions (the varieties and the contents of the auxiliary agents in the compositions with different proportions are the same). The cold resistance test is carried out on potted plants by using compositions with different proportions, corresponding single agents and blank controls are arranged, the treatments are diluted by 500 times, seedlings of peanuts, beans and cucumbers are sprayed once (the seedlings are all in 4-6 leaf stage), more than 20 plants are sprayed on each crop, the crops are sprayed for 24 hours and then put into a constant temperature box with the temperature of 4 ℃ for low-temperature growth, and the crops are taken out after 34 hours and put under the room temperature (about 25 ℃) for cold damage result investigation. The grading standard and the calculation formula of the cold damage of the crop leaves are as follows, and the results are shown in Table 1.
Level 0: no cold damage symptom
Level 1: slight shrinkage of the leaves, slight dehydration of one third of the leaves, normal heart leaves
And 2, stage: a shrunken leaf, one third of the leaf with water loss and normal heart leaves
And 3, level: a shriveled leaf, two thirds of the leaf with water loss and a normal heart
4, level: the whole leaf loses water and wilts, the heart leaves lose water slightly, and the plant can recover at normal temperature
And 5, stage: the whole leaf is severely withered due to water loss and can not be recovered at normal temperature
TABLE 1 Cold resistance test of compositions of chitosan and S-abscisic acid in different proportions on crops
The results of the cold resistance test of the compositions of chitosan and S-abscisic acid in different proportions on crops are shown in Table 1. The results show that in the effect of the composition containing 4% of chitosan and different contents of S-abscisic acid on 3 crops, the indexes of the compositions of the chitosan and the S-abscisic acid in different proportions on the cold damage of the crops are lower than that of a single-dose control, and the cold-resistant effect is obvious compared with that of other 2 proportions when the proportion of the chitosan and the S-abscisic acid is 40: 1. EXAMPLE 2 synergistic adjuvant screening of compositions containing Chitosan and S-abscisic acid
1. Preparation of compositions containing different synergists
4.0% chitosan S-abscisic acid microemulsion containing 10% sodium silicate is prepared. Accurately weighing 3.9 g of chitosan, fully dissolving with 6 g of lactic acid, adding 0.1 g of S-abscisic acid, fully mixing, adding 10 g of sodium silicate and 80 g of Tween, stirring the mixture in a stirrer, and supplementing water to 100g to obtain the stable-quality 4.0% chitosan-S-abscisic acid microemulsion containing 10% of sodium silicate by mass detection.
Prepared by the same method, 4.0 percent chitosan-S-abscisic acid microemulsion (3.9 percent chitosan and 0.1 percent S-abscisic acid) containing 0.5 percent salicylic acid and 4.0 percent chitosan-S-abscisic acid microemulsion (3.9 percent chitosan and 0.1 percent S-abscisic acid) containing 10 percent chelated calcium.
2. Synergistic adjuvant screening of 4.0% Chitosan-S-abscisic acid (3.9% Chitosan + 0.1% S-abscisic acid) microemulsion
A builder screening test was conducted on a microemulsion of the composition 4.0% Chitosan.S-abscisic acid (3.9% Chitosan + 0.1% S-abscisic acid). Setting corresponding single agent and blank control, spraying more than 20 plants on seedlings of corn, peanut, bean and cucumber once by diluting the composition containing 4.0% chitosan.S-abscisic acid of different synergistic additives by 500 times (the seedlings are all in 4-6 leaf stage), putting the seedlings into a constant temperature box at 4 ℃ for low-temperature growth 24 hours after spraying treatment, taking out the seedlings after 24 hours, putting the seedlings at room temperature (about 25 ℃) for cold damage result investigation, and carrying out grading standard and calculation formula of the cold damage of the leaves of the crops as example 1, wherein the results are shown in table 2.
TABLE 24.0% potentiator screening test for Chitosan S-abscisic acid
The results of the 4.0% screening test for the potentiator of chitosan-S-abscisic acid are shown in Table 2. As a result, it was found that the cold injury index of 4.0% chitosan-S-abscisic acid containing different synergists was lower than that of 4.0% chitosan-S-abscisic acid and that of the single-agent control in all of the 4 crops tested, and the cold-resistant effect of 4.0% chitosan-S-abscisic acid containing 10% sodium silicate was significant compared with that of the other treatments, and the cold-resistant effect of the 4.0% chitosan-S-abscisic acid containing 10% chelated calcium was inferior to that of the composition containing 4.0% chitosan-S-abscisic acid containing 10% chelated calcium.
Example 3 microemulsion compositions containing 10% sodium silicate were further tested for cold resistance in plants
The cold damage test of the compositions containing 10 percent of sodium silicate, chitosan and S-abscisic acid in different proportions on plants is further tested: the cold resistance test is carried out on potted plants by using the composition, corresponding single agents and blank controls are set, crop seedlings are sprayed once by 500 times (4-6 leaf period for each seedling), more than 20 plants are sprayed on each crop, the crop is sprayed before low-temperature treatment, the crop seedlings are taken out after the low-temperature treatment is carried out for a period of time and placed at room temperature (about 25 ℃) for cold damage result investigation, the plant height increment (cm) and the average relative chlorophyll content (SPAD) of corn are tested, the grading standard and the calculation formula of the cold damage of the crop leaves are as in example 1, and the results are shown in Table 3.
Table 3 further tests of the Cold resistance of compositions of chitosan and S-abscisic acid in different ratios to crops
The composition of chitosan and S-abscisic acid with different mixture ratio of 10% sodium silicate was tested for further cold resistance test on crops, and the mixture ratio of chitosan and S-abscisic acid was 29:1, 39:1 and 49:1, and the results are shown in Table 3. The results show that in several tested crop seedlings, the plant height increment of the composition containing chitosan and S-abscisic acid in different proportions on corn is lower than that of a control medicament and a clear water control, but the relative chlorophyll content is higher than that of a single-dose control, so that the composition containing the chitosan and the S-abscisic acid cannot cause the excessive growth of crops to promote the cold resistance of the crops during the cold damage period, but promotes the chlorophyll content of the crops to be increased; the cold damage indexes of peanuts, kidney beans and cucumbers are lower than those of single-dose control, and the cold-resistant effect is obvious compared with that of other ratios of 29:1 to 49:1 when the ratio of chitosan to S-abscisic acid is 39: 1.
EXAMPLE 4 screening of application concentrations of 4.0% Chitosan-S-abscisic acid microemulsion containing potentiator
Screening of the application concentration of 4.0% chitosan-S-abscisic acid (3.9% chitosan + 0.1% S-abscisic acid) microemulsion containing 10% sodium silicate was developed. Diluting chitosan, S-abscisic acid 4.0% and sodium silicate 10% in different concentrations, spraying onto eggplant, tomato, hot pepper and melon seedlings once (4-6 leaf period for each seedling), spraying over 10 plants for each crop, putting into a 4 ℃ constant temperature box for low-temperature growth 48 hours after spraying treatment, taking out after 36 hours, putting into room temperature (about 25 ℃) for cold damage result investigation, wherein the grading standard and calculation formula of the cold damage of the crop leaves are as in example 1, and the results are shown in Table 4.
TABLE 4 compositions of 4.0% Chitosan-S-abscisic acid + 10% sodium silicate with varying concentrations of synergist for crop cold resistance test
Compositions of 4.0% chitosan-S-abscisic acid + 10% sodium silicate at various concentrations were tested for cold-resistance in crops and the results are shown in table 4. As a result, the composition has obvious cold-resistant effect on 4 crops after being diluted by 250-2000 times in 4 crops to be tested; the effect is better when the plant is diluted by 250 times and 1000 times, and the plants can recover normal growth after being placed at room temperature for 72 hours. In practical application, the composition is recommended to be diluted by 250-fold and sprayed by 1000-fold for 1-3 days before cold injury comes, and if the temperature is lower than 4 ℃ and the temperature is lower than 2 days, the composition is recommended to be diluted by 250-fold and sprayed by 500-fold for application.
Claims (10)
1. An agricultural composition containing chitosan and S-abscisic acid is characterized in that the composition contains 5-40% of sodium silicate, the relative molecular mass of the chitosan is 5000-20000 daltons, and the mass part ratio of the chitosan to the S-abscisic acid is 10:1-50: 1.
2. Composition according to claim 1, characterized in that the sodium silicate content of the composition is 10%.
3. The composition of claim 2, wherein the mass portion ratio of chitosan to S-abscisic acid is 39: 1.
4. A composition according to claim 3, characterized in that the sum of the masses of chitosan and S-abscisic acid is between 5 and 80% of the total mass of the composition.
5. A composition according to claim 4, characterized in that the sum of the masses of chitosan and S-abscisic acid is between 20 and 50% of the total mass of the composition.
6. Composition according to claim 4 or 5, characterized in that the composition is formulated as a microemulsion, aqueous solution, suspension, aqueous emulsion, wettable powder, water dispersible granule.
7. The composition of claim 6, wherein the composition is in the form of a microemulsion.
8. Use of the composition of claim 7 for cold resistance in plants.
9. Use according to claim 8, characterized in that the composition is applied by spray diluted 250-fold and 1000-fold before cold damage comes.
10. Use according to claim 9, characterized in that the composition is applied by spraying diluted 250-fold to 500-fold 1-3 days before the onset of cold damage.
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| CN114982761B (en) * | 2022-06-13 | 2024-03-08 | 西北农林科技大学 | Application of streptavidin-free glucan in preparation of plant immunity elicitor |
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| CN102701861A (en) * | 2012-06-21 | 2012-10-03 | 魏玉芳 | Plant nutrient liquid fertilizer |
| CN106665205A (en) * | 2016-12-09 | 2017-05-17 | 河池市农业科学研究所 | Method for improving drought resistance of sugarcane |
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| CN102701861A (en) * | 2012-06-21 | 2012-10-03 | 魏玉芳 | Plant nutrient liquid fertilizer |
| CN106665205A (en) * | 2016-12-09 | 2017-05-17 | 河池市农业科学研究所 | Method for improving drought resistance of sugarcane |
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Denomination of invention: A composition containing chitosan and s-inducer and its application Effective date of registration: 20220304 Granted publication date: 20210611 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2019460000007 |
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Address after: Zhengye Square, No. 7 Jinlian Road, Longhua District, Hainan Province, 571923 Patentee after: Hainan Zhengye Biotechnology Co.,Ltd. Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |