CN109966348A - Snippings Fructus Piperis extract and its pharmaceutical composition and its application - Google Patents
Snippings Fructus Piperis extract and its pharmaceutical composition and its application Download PDFInfo
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- CN109966348A CN109966348A CN201910282006.5A CN201910282006A CN109966348A CN 109966348 A CN109966348 A CN 109966348A CN 201910282006 A CN201910282006 A CN 201910282006A CN 109966348 A CN109966348 A CN 109966348A
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- extract
- snippings
- pepper
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- pxt6
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- 239000000284 extract Substances 0.000 title claims abstract description 150
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 7
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 72
- 239000006002 Pepper Substances 0.000 claims abstract description 72
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 72
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 72
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 72
- 239000003814 drug Substances 0.000 claims abstract description 17
- 239000002537 cosmetic Substances 0.000 claims abstract description 15
- 230000003110 anti-inflammatory effect Effects 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 235000013361 beverage Nutrition 0.000 claims abstract description 9
- 235000013402 health food Nutrition 0.000 claims abstract description 9
- 241000722363 Piper Species 0.000 claims description 73
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 239000000203 mixture Substances 0.000 claims description 25
- 241000628997 Flos Species 0.000 claims description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 21
- 241000220317 Rosa Species 0.000 claims description 19
- 239000002038 ethyl acetate fraction Substances 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 16
- 244000197580 Poria cocos Species 0.000 claims description 15
- 235000008599 Poria cocos Nutrition 0.000 claims description 15
- 239000000401 methanolic extract Substances 0.000 claims description 14
- 241001092040 Crataegus Species 0.000 claims description 13
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims description 13
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims description 13
- 235000009685 Crataegus X maligna Nutrition 0.000 claims description 13
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims description 13
- 235000009486 Crataegus bullatus Nutrition 0.000 claims description 13
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims description 13
- 235000009682 Crataegus limnophila Nutrition 0.000 claims description 13
- 235000004423 Crataegus monogyna Nutrition 0.000 claims description 13
- 235000002313 Crataegus paludosa Nutrition 0.000 claims description 13
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims description 13
- 240000002853 Nelumbo nucifera Species 0.000 claims description 13
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 13
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 235000019441 ethanol Nutrition 0.000 claims description 11
- 238000004064 recycling Methods 0.000 claims description 11
- 235000001188 Peltandra virginica Nutrition 0.000 claims description 10
- 238000013461 design Methods 0.000 claims description 10
- 238000002137 ultrasound extraction Methods 0.000 claims description 9
- 239000000341 volatile oil Substances 0.000 claims description 9
- 230000003064 anti-oxidating effect Effects 0.000 claims description 5
- 239000003937 drug carrier Substances 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 238000003810 ethyl acetate extraction Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 235000020717 hawthorn extract Nutrition 0.000 claims description 2
- 210000000582 semen Anatomy 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 1
- 239000005864 Sulphur Substances 0.000 claims 1
- 230000005611 electricity Effects 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 229940079593 drug Drugs 0.000 abstract description 9
- 230000003078 antioxidant effect Effects 0.000 abstract description 8
- 239000003963 antioxidant agent Substances 0.000 abstract description 4
- 235000006708 antioxidants Nutrition 0.000 abstract description 4
- 244000203593 Piper nigrum Species 0.000 abstract 1
- 238000000638 solvent extraction Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 description 10
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 7
- 239000000546 pharmaceutical excipient Substances 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000002000 scavenging effect Effects 0.000 description 3
- 241000722824 Ardisia crenata Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- NTNWOCRCBQPEKQ-YFKPBYRVSA-N N(omega)-methyl-L-arginine Chemical compound CN=C(N)NCCC[C@H](N)C(O)=O NTNWOCRCBQPEKQ-YFKPBYRVSA-N 0.000 description 2
- NTNWOCRCBQPEKQ-UHFFFAOYSA-N NG-mono-methyl-L-arginine Natural products CN=C(N)NCCCC(N)C(O)=O NTNWOCRCBQPEKQ-UHFFFAOYSA-N 0.000 description 2
- 235000014676 Phragmites communis Nutrition 0.000 description 2
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- 150000001875 compounds Chemical class 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
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- 239000000243 solution Substances 0.000 description 2
- WCBPJVKVIMMEQC-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 WCBPJVKVIMMEQC-UHFFFAOYSA-N 0.000 description 1
- LCPVQAHEFVXVKT-UHFFFAOYSA-N 2-(2,4-difluorophenoxy)pyridin-3-amine Chemical compound NC1=CC=CN=C1OC1=CC=C(F)C=C1F LCPVQAHEFVXVKT-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940124599 anti-inflammatory drug Drugs 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000003978 infusion fluid Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000012669 liquid formulation Substances 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000005453 pelletization Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- CHQMHPLRPQMAMX-UHFFFAOYSA-L sodium persulfate Substances [Na+].[Na+].[O-]S(=O)(=O)OOS([O-])(=O)=O CHQMHPLRPQMAMX-UHFFFAOYSA-L 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 230000004083 survival effect Effects 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/67—Piperaceae (Pepper family), e.g. Jamaican pepper or kava
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A—HUMAN NECESSITIES
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- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Abstract
Snippings Fructus Piperis extract is provided and its pharmaceutical composition is applied with it.Extract PDH and PXT5 is prepared with solvent extraction and extraction in dry snippings pepper stem or leaf.PXT5 has stronger anti-inflammatory activity.PDH has anti-oxidant and anti-inflammatory double action.PDH and PXT5 and its different combinations, can be used to prepare beverage, health food, cosmetics and drug.
Description
Technical field:
The invention belongs to plant resources utilization field more particularly to snippings Fructus Piperis extract and its pharmaceutical composition and its
Beverage, health food, cosmetics, the application in drug field.
Background technique:
Snippings pepper (Piper magen) is Piperaceae pepper platymiscium, is that In Xishuangbanna of Yunnan ethnic group is edible
A kind of spice berry [a.Yang J, Wang Y-H, Luo J-F, Long C-L.Piper magen (Piperaceae), a new
Species from Yunnan, China.Phytotaxa, 2017,316 (2): 189-194.b. journey must the strong Xishuangbanna minority
National flavorant plant fragrance flavor and cosmetic, 1991, (3), 27-31；C. the sub- heat in the Yunnan Cheng Biqiang, the Yu Xuejian torrid zone
The band Kunming spice berry: publishing house, Yunnan University .1995；D. the Xishuangbanna Li Yanhui, Pei Shengji, Xu Zaifu higher plant name
Record the Kunming: Yunnan's Ethnic publishing house .1996].Patent " cuttage breeding method of snippings " (CN107484535A) and " national perfume
The rapid propagation method of tannin plant snippings " (CN107410033A) reports the propagation method to snippings pepper；Patent " snippings essence
Oil and its extraction and chemical composition analysis method " (CN201710059926.1) reports the application of snippings pepper essential oil.But fiber crops
Root pepper is upper in anti-oxidant and anti-inflammatory activity and its application, and there is not been reported.
Summary of the invention:
The present invention in view of the above-mentioned problems existing in the prior art, provides snippings snippings Fructus Piperis extract and its pharmaceutical composition
With its application in beverage, health food, cosmetics, drug field.
In order to realize above-mentioned purpose of the invention, the present invention provides the following technical solutions:
Snippings Fructus Piperis extract is snippings pepper stem methanolic extract Ethyl acetate fraction PDH or/and snippings pepper
Leaf extract PXT5 or/and snippings pepper stem water extract PXT14 or/and snippings pepper essential oil PXT15, they are by following methods
It is prepared:
Snippings pepper stem methanolic extract Ethyl acetate fraction PDH is to use dry snippings pepper stem, after crushing, is added
Enter and impregnated under methanol 10L room temperature, extracted 4 times, obtains extract after filtrate recycling design, be suspended in 2L water, ethyl acetate extraction
It takes 3 times；
Snippings pepper leaf extract PXT5 is to use dry snippings pepper leaf, and 90% ethyl alcohol of 200mL is added, 60 DEG C, ultrasonic
It extracts 30 minutes, recycling design obtains extract；
Snippings pepper stem water extract PXT14 is crushed with dry snippings pepper stem, is weighed 200 grams, is transferred to 2 liters
In round-bottomed flask, 1.5 liters of water are added into round-bottomed flask, is shaken up, with water in electric jacket heating round-bottomed flask to boiling, it is small to extract 6
When, filtering, filtrate recycling design obtains snippings pepper stem extraction；
Snippings pepper essential oil PXT15 is to use snippings stem drying and crushing, weighs 200 grams of smashed snippings sample, is transferred to 2
In the round-bottomed flasks risen, 1.5 liters of water are added into round-bottomed flask, shakes up, glass extraction of essential oil device and condenser pipe is set on round-bottomed flask,
With water in electric jacket heating round-bottomed flask to boiling, keep slightly boiled, vapor air-distillation 6 hours, the liquid nothing distilled out
Aqueous sodium persulfate is dry, obtains snippings pepper essential oil.
Pharmaceutical composition, wherein containing the snippings Fructus Piperis extract and pharmaceutically acceptable carrier.
The application of the snippings Fructus Piperis extract in medicine preparation.
The snippings Fructus Piperis extract is preparing application anti-inflammatory, in anti-oxidation medicine.
The snippings Fructus Piperis extract is preparing cosmetics, beverage, the application in health food.
Composition PXT11, PXT13, PXT16, PXT17, PXT18, PXT19 containing the snippings Fructus Piperis extract,
PXT20, PXT21, PXT22, PXT23, PXT24, PXT25, PXT26, PXT27, PXT28, PXT29, PXT30, they are by following
Formula is formed:
PXT11 snippings pepper leaf, lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, is formulated as PXT5:
PXT7:PXT8:PXT9=1:1:1:1；
PXT13 snippings pepper leaf, rose, lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, formula
For PXT5:PXT6:PXT7:PXT8:PXT9=1:1:1:1:1；
PXT16 rose, the unification of snippings pepper stem water extract group: extract presses quality proportioning, is formulated as PXT6:
PXT14=1:9；
PXT17 rose, snippings pepper stem water extract combination two: extract presses quality proportioning, is formulated as PXT6:
PXT14=2:8；
PXT18 rose, snippings pepper stem water extract combination three: extract presses quality proportioning, is formulated as PXT6:
PXT14=5:5；
PXT19 rose, snippings pepper stem water extract combination four: extract presses quality proportioning, is formulated as PXT6:
PXT14=8:2；
PXT20 rose, snippings pepper stem water extract combination five: extract presses quality proportioning, is formulated as PXT6:
PXT14=9:1；
PXT21 snippings pepper leaf, the unification of Flos Rosae Rugosae extract group: extract presses quality proportioning, is formulated as PXT5:PXT6=
9:1；
PXT22 snippings pepper leaf, Flos Rosae Rugosae extract combination two: extract presses quality proportioning, is formulated as PXT5:PXT6=
8:2；
PXT23 snippings pepper leaf, Flos Rosae Rugosae extract combination three: extract presses quality proportioning, is formulated as PXT5:PXT6=
5:5；
PXT24 snippings pepper leaf, Flos Rosae Rugosae extract combination four: extract presses quality proportioning, is formulated as PXT5:PXT6=
2:8；
PXT25 snippings pepper leaf, Flos Rosae Rugosae extract combination five: extract presses quality proportioning, is formulated as PXT5:PXT6=
1:9；
PXT26 snippings pepper stem methanolic extract Ethyl acetate fraction, the unification of Flos Rosae Rugosae extract group: extract is pressed
Quality proportioning is formulated as PDH:PXT6=9:1；
PXT27 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination two: extract is pressed
Quality proportioning is formulated as PDH:PXT6=8:2；
PXT28 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination three: extract is pressed
Quality proportioning is formulated as PDH:PXT6=5:5；
PXT29 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination four: extract is pressed
Quality proportioning is formulated as PDH:PXT6=2:8；
PXT30 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination five: extract is pressed
Quality proportioning is formulated as PDH:PXT6=1:9.
The composition is preparing application anti-inflammatory, in anti-oxidation medicine.
The composition is preparing cosmetics, beverage, the application in health food.
A kind of the composition PXT10 or PXT12 of extract are made of following extraction raw materials and proportion:
PXT10 lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, is formulated as PXT7:PXT8:PXT9=
1:1:1；
PXT12 rose, lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, is formulated and is
PXT6:PXT7:PXT8:PXT9=1:1:1:1,
Wherein extract raw material the preparation method is as follows:
Flos Rosae Rugosae extract PXT6: dry rose, addition 90% ethyl alcohol of 200ML, 60 DEG C, ultrasonic extraction 30 minutes,
Extract is obtained after recycling design；
Semen Nelumbinis extract PXT7: dry lotus seeds are added 90% ethyl alcohol of 200ML, 60 DEG C, ultrasonic extraction 30 minutes, recycle
Extract is obtained after solvent；
Haw thorn extract PXT8: dry hawthorn, addition 90% ethyl alcohol of 200ML, 60 DEG C, ultrasonic extraction 30 minutes.Recycling
Extract is obtained after solvent；
Tuckahoe extracts PXT9: dry Poria cocos is added 90% ethyl alcohol of 200ML, 60 DEG C, ultrasonic extraction 30 minutes, recycles
Extract is obtained after solvent.
Composition application in preparing anti-inflammatory drugs is preparing cosmetics, beverage, answering in health food
With.
The present invention provides above-mentioned extract and composition for the first time, finds it with anti-inflammatory activity and antioxidant activity.Its
Pharmacological activity is high, and effectiveness is good, and extract of the invention and composition generate the IC inhibited to NO50Value activity is high, and activity is significant
Higher than positive control, realize that it is preparing the application in drug and cosmetics.
Pharmaceutically acceptable carrier of the present invention refers to the pharmaceutical carrier of pharmaceutical field routine, for example, water, grape
Sugar, lactose, Arabic gum etc. and it is suitble to used in the preparation for preparing solid, semisolid, liquid or aerosol form other
Carrier.In addition composition can contain stabilizer, thickener and/or colorant and fragrance.
The composition that extract and its pharmaceutically acceptable carrier of the invention is prepared can be orally or without mouth
Administration, dosage are had nothing in common with each other because of drug difference, and for adult, daily 1-100mg is appropriate.
When oral administration, make extract or composition and conventional medicinal adjuvant such as excipient, solution agent, bonding first
Agent, lubricant, antioxidant, coating agent, colorant, aromatic, surfactant etc. mixing, be made into granule, capsule,
The administration of the forms such as tablet: it can be administered in the form of injection, infusion solution or suppository etc. when non-oral administration.When preparing above-mentioned preparation,
Conventional preparation technique can be used.
Specific embodiment:
Essentiality content of the invention is further illustrated with the embodiment of the present invention below, but this is not limited with this
Invention.
Embodiment 1:
The various extracts of the present invention, formula of various compositions and preparation method thereof are shown in Table 1.
The formula of the 1. various extracts of the present invention of table and its preparation
Embodiment 2:
Anti-inflammatory (NO, which is generated, to be inhibited) activity test method: by RAW264.7 cell inoculation to 96 orifice plates, with 1 μ g/mL LPS
Induction stimulation is carried out, while untested compound (final concentration 2 times of dilutions since 50 μ g/mL set 5 gradients altogether) processing is added, if
It sets and is compareed without medicine group and L-NMMA positive drug group.Culture medium detection NO is taken to generate after cell pellet overnight culture, in 570nm
Place's measurement light absorption value.MTS is added in remaining culture medium and carries out cell survival rate detection, excludes the toxicity shadow of compound on intracellular
It rings.
NO generates inhibiting rate (%)=(non-drug processing group OD570Sample sets OD570)/non-drug processing group OD570×
100%.
IC50(50%concentration of inhibition) is calculated by Reed&Muench method.
Extract generates inhibiting effect to NO and is shown in Table 2 and table 3.Snippings pepper stem extraction Ethyl acetate fraction
(PDH) and snippings pepper leaf extract (PXT5), there is significant anti-inflammatory activity, the IC inhibited to NO production50Value is respectively
24.63 ± 1.17 μ g/mL and 30.74 ± 0.70 μ g/mL.Combination PXT21 and PXT22 also has stronger inhibition to make NO generation
With.
2 extract of table generates inhibiting rate to NO when concentration is 50 μ g/mL or 25 μ g/mL
3 extract of table generates the IC inhibited to NO50Value
| Extract number | IC50Value |
| L-NMMA (positive control) | 46.99±1.70μM |
| PDH | 24.63±1.17μg/mL |
| PXT5 | 30.74±0.70μg/mL |
Embodiment 3:
Anti-oxidant (DPPH radicals scavenging) activity determination method: it utilizes DPPH (1,1- diphenyl -2- trinitrophenyl-hydrazine)
Method evaluates extract antioxidant activity in vitro.DPPH is a kind of very stable free radical centered on nitrogen, is had at 515nm strong
Absorption peak, in the presence of having free radical scavenger, the proton that DPPH can be provided with scavenger, which combines, forms DPPH-H, to make molten
Liquid color becomes yellow from purple, and the absorbance at 515nm becomes smaller.The extraction with antioxidant activity in vitro is screened with this
Object.By sample to be tested and DPPH (final concentration of 100 μM) hybrid reaction, 3 repeating holes are set, while the sky of not drug containing is set
White control wells and Trolox Positive control wells, 30 DEG C, 1h, microplate reader measures OD value, and Detection wavelength 515nm is calculated anti-
Oxygenation efficiency.
Antioxygen rate (%)=(1- experimental port OD515/ blank well OD515) × 100%
IC50(50%inhibitory concentration) is calculated by Reed&Muench method.
Extract is shown in Table 4 and table 5 to DPPH free radical scavenging activity.Snippings pepper stem extraction Ethyl acetate fraction
(PDH) have compared with strong antioxidant action.
4 extract of table is in 100 μ g/mL of concentration to DPPH scavenging capacity
| Extract number | Concentration (μ g/mL) | Antioxygen rate (%) |
| Trolox (positive control) | 25 | 94.9 |
| PDH | 100 | 60.9 |
| PXT5 | 100 | 4.1 |
| PXT6 | 100 | 92.9 |
| PXT7 | 100 | 0.8 |
| PXT8 | 100 | 30.1 |
| PXT9 | 100 | 2.6 |
| PXT10 | 100 | 16.6 |
| PXT11 | 100 | 14.8 |
| PXT12 | 100 | 93.6 |
| PXT13 | 100 | 92.7 |
| PXT14 | 100 | 21.5 |
| PXT15 | 100 | 4.8 |
| PXT16 | 100 | 86.7 |
| PXT17 | 100 | 88.4 |
| PXT18 | 100 | 88.6 |
| PXT19 | 100 | 91.6 |
| PXT20 | 100 | 92.1 |
| PXT21 | 100 | 62.5 |
| PXT22 | 100 | 87.4 |
| PXT23 | 100 | 90.2 |
| PXT24 | 100 | 91.5 |
| PXT25 | 100 | 92.8 |
| PXT26 | 100 | 92.8 |
| PXT27 | 100 | 93.0 |
| PXT28 | 100 | 93.1 |
| PXT29 | 100 | 92.7 |
| PXT30 | 100 | 93.5 |
IC of 5 extract of table to DPPH scavenging capacity50Value
| Extract number | IC50It is worth (μ g/mL) |
| Trolox (positive control) | 4.08±0.05 |
| PXT6 | 5.45±0.44 |
| PXT12 | 17.01±0.46 |
| PXT13 | 25.96±2.01 |
| PXT16 | 33.96±1.94 |
| PXT17 | 27.95±1.39 |
| PXT18 | 9.38±0.05 |
| PXT19 | 5.49±0.25 |
| PXT20 | 5.04±0.07 |
| PXT21 | 80.94±1.56 |
| PXT22 | 32.36±1.54 |
| PXT23 | 9.59±0.12 |
| PXT24 | 6.94±0.12 |
| PXT25 | 5.15±0.42 |
| PXT26 | 48.03±2.56 |
| PXT27 | 26.02±3.45 |
| PXT28 | 9.34±0.72 |
| PXT29 | 6.31±0.03 |
| PXT30 | 5.51±0.51 |
Embodiment 4:
Dry snippings pepper leaf or 1~2 gram of stem crush, and boiled water is taken after mixing it with water or pan-fried water is for oral administration.
Embodiment 5:
Dry snippings pepper leaf or 1~2 gram of stem, crush by 3~6 grams of rose, and boiled water is taken after mixing it with water or pan-fried water is for oral administration.
Embodiment 6:
Dry snippings pepper leaf or 1~2 gram of stem, 9~12 grams of hawthorn, 10~15 grams of Poria cocos, crush by 6~15 grams of lotus seeds,
Boiled water is taken after mixing it with water or pan-fried water is for oral administration.
Embodiment 7:
Dry snippings pepper leaf or 1~2 gram of stem, 3~6 grams of rose, 6~15 grams of lotus seeds, 9~12 grams of hawthorn, Poria cocos
It 10~15 grams, crushes, boiled water is taken after mixing it with water or pan-fried water is for oral administration.
Embodiment 8:
3~6 grams of dry rose, 9~12 grams of hawthorn, 10~15 grams of Poria cocos, crushes, boiled water punching by 6~15 grams of lotus seeds
Clothes or pan-fried water are for oral administration.
Embodiment 9:
The preparation of tablet:
Various extracts or various compositions of the invention are first made by the formula and preparation method of embodiment 1, it is each by it
From independent or any mixing, with excipient weight than excipient, pelletizing press sheet is added for the ratio of 1:5-1:10.
Embodiment 10:
The preparation of oral liquid formulations:
Various extracts or various compositions of the invention are first made by the formula and preparation method of embodiment 1, it is each by it
From independent or any mixing, routinely oral solution is made in oral solution preparation method.
Embodiment 11:
The preparation of capsule, granule or electuary:
Various extracts or various compositions of the invention are first made by the formula and preparation method of embodiment 1, it is each by it
From independent or any mixing, in its with excipient weight than excipient is added for the ratio of 5:1, capsule or granule or punching is made
Agent.
Embodiment 12:
Skin whitener formula (W%) containing various extracts or various compositions of the invention shown in table 1:
One of various extracts or various compositions shown in table 1 or any mixing 0.05
The cosmetics of the above-mentioned formula of the present invention are made in the method for routinely making cosmetics.
Embodiment 13:
Emulsion formulations (W%) containing various extracts or various compositions of the invention shown in table 1:
One of various extracts or various compositions shown in table 1 or any mixing 0.05
The cosmetics of the above-mentioned formula of the present invention are made in the method for routinely making cosmetics.
Explanation above-mentioned should be understood as being only to illustrate the present invention.Various selections and amendment can be by one skilled in the art
It is designed without departing substantially from the present invention.Thus, it is intended that including all this selections fallen within the scope of the appended claims
With amendment and variation.
Claims (10)
1. snippings Fructus Piperis extract is snippings pepper stem methanolic extract Ethyl acetate fraction PDH or/and snippings pepper leaf
Extract PXT5 or/and snippings pepper stem water extract PXT14 or/and snippings pepper essential oil PXT15, it is characterised in that they by
Following methods are prepared:
Snippings pepper stem methanolic extract Ethyl acetate fraction PDH is with dry snippings pepper stem, after crushing, addition first
It impregnates, extracts 4 times under alcohol 10L room temperature, obtain extract after filtrate recycling design, be suspended in 2L water, ethyl acetate extraction 3
It is secondary；
Snippings pepper leaf extract PXT5 is to use dry snippings pepper leaf, addition 200mL90% ethyl alcohol, 60 DEG C, ultrasonic extraction
30 minutes, recycling design obtained extract；
Snippings pepper stem water extract PXT14 is crushed with dry snippings pepper stem, is weighed 200 grams, is transferred to 2 liters of round bottom
In flask, 1.5 liters of water are added into round-bottomed flask, is shaken up, with water in electric jacket heating round-bottomed flask to boiling, extracted 6 hours, mistake
Filter, filtrate recycling design obtain snippings pepper stem extraction；
Snippings pepper essential oil PXT15 is to use snippings stem drying and crushing, weighs 200 grams of smashed snippings sample, is transferred to 2 liters
In round-bottomed flask, 1.5 liters of water are added into round-bottomed flask, shakes up, glass extraction of essential oil device and condenser pipe, electricity consumption is set on round-bottomed flask
Hot jacket heats water in round-bottomed flask and keeps slightly boiled to boiling, and vapor air-distillation 6 hours, the anhydrous sulphur of the liquid distilled out
Sour sodium is dry, obtains snippings pepper essential oil.
2. pharmaceutical composition, wherein containing snippings Fructus Piperis extract described in claim 1 and pharmaceutically acceptable carrier.
3. the application of snippings Fructus Piperis extract described in claim 1 in medicine preparation.
4. snippings Fructus Piperis extract described in claim 1 is preparing application anti-inflammatory, in anti-oxidation medicine.
5. snippings Fructus Piperis extract described in claim 1 is preparing cosmetics, beverage, the application in health food.
6. composition PXT11, PXT13, PXT16, PXT17, PXT18 containing snippings Fructus Piperis extract described in claim 1,
PXT19, PXT20, PXT21, PXT22, PXT23, PXT24, PXT25, PXT26, PXT27, PXT28, PXT29, PXT30, it is special
Sign is that it is made of following formulas:
PXT11 snippings pepper leaf, lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, is formulated as PXT5:
PXT7:PXT8:PXT9=1:1:1:1；
PXT13 snippings pepper leaf, rose, lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, is formulated and is
PXT5:PXT6:PXT7:PXT8:PXT9=1:1:1:1:1；
PXT16 rose, the unification of snippings pepper stem water extract group: extract presses quality proportioning, is formulated as PXT6:PXT14=
1:9；
PXT17 rose, snippings pepper stem water extract combination two: extract presses quality proportioning, is formulated as PXT6:PXT14=
2:8；
PXT18 rose, snippings pepper stem water extract combination three: extract presses quality proportioning, is formulated as PXT6:PXT14=
5:5；
PXT19 rose, snippings pepper stem water extract combination four: extract presses quality proportioning, is formulated as PXT6:PXT14=
8:2；
PXT20 rose, snippings pepper stem water extract combination five: extract presses quality proportioning, is formulated as PXT6:PXT14=
9:1；
PXT21 snippings pepper leaf, the unification of Flos Rosae Rugosae extract group: extract presses quality proportioning, is formulated as PXT5:PXT6=9:1；
PXT22 snippings pepper leaf, Flos Rosae Rugosae extract combination two: extract presses quality proportioning, is formulated as PXT5:PXT6=8:2；
PXT23 snippings pepper leaf, Flos Rosae Rugosae extract combination three: extract presses quality proportioning, is formulated as PXT5:PXT6=5:5；
PXT24 snippings pepper leaf, Flos Rosae Rugosae extract combination four: extract presses quality proportioning, is formulated as PXT5:PXT6=2:8；
PXT25 snippings pepper leaf, Flos Rosae Rugosae extract combination five: extract presses quality proportioning, is formulated as PXT5:PXT6=1:9；
PXT26 snippings pepper stem methanolic extract Ethyl acetate fraction, the unification of Flos Rosae Rugosae extract group: extract presses quality
Proportion, is formulated as PDH:PXT6=9:1；
PXT27 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination two: extract presses quality
Proportion, is formulated as PDH:PXT6=8:2；
PXT28 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination three: extract presses quality
Proportion, is formulated as PDH:PXT6=5:5；
PXT29 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination four: extract presses quality
Proportion, is formulated as PDH:PXT6=2:8；
PXT30 snippings pepper stem methanolic extract Ethyl acetate fraction, Flos Rosae Rugosae extract combination five: extract presses quality
Proportion, is formulated as PDH:PXT6=1:9.
7. composition as claimed in claim 6 is preparing application anti-inflammatory, in anti-oxidation medicine.
8. composition as claimed in claim 6 is preparing cosmetics, beverage, the application in health food.
9. the composition PXT10 or PXT12 of a kind of extract, it is characterised in that it is by following extraction raw materials and proportion institute's group
At:
PXT10 lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, is formulated as PXT7:PXT8:PXT9=1:1:
1；
PXT12 rose, lotus seeds, hawthorn, tuckahoe extracts combination: extract presses quality proportioning, is formulated as PXT6:PXT7:
PXT8:PXT9=1:1:1:1,
Wherein extract raw material the preparation method is as follows:
Flos Rosae Rugosae extract PXT6: dry rose is added 200ML90% ethyl alcohol, 60 DEG C, ultrasonic extraction 30 minutes, recycles
Extract is obtained after solvent；
Semen Nelumbinis extract PXT7: dry lotus seeds, addition 200ML90% ethyl alcohol, 60 DEG C, ultrasonic extraction 30 minutes, recycling design
Extract is obtained afterwards；
Haw thorn extract PXT8: dry hawthorn, addition 200ML90% ethyl alcohol, 60 DEG C, ultrasonic extraction 30 minutes.Recycling design
Extract is obtained afterwards；
Tuckahoe extracts PXT9: dry Poria cocos, addition 200ML90% ethyl alcohol, 60 DEG C, ultrasonic extraction 30 minutes, recycling design
Extract is obtained afterwards.
10. composition as claimed in claim 9 is preparing application anti-inflammatory, in anti-oxidation medicine, prepare cosmetics, beverage,
Application in health food.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111718799A (en) * | 2020-06-30 | 2020-09-29 | 三沙南海美源岛生物科技有限公司 | Method for extracting pepper leaf essential oil by eutectic solvent method and ultrasonic-assisted distillation |
| CN111718798A (en) * | 2020-06-30 | 2020-09-29 | 三沙南海美源岛生物科技有限公司 | Method for extracting pepper leaf essential oil by microwave-assisted distillation through eutectic solvent method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701321A (en) * | 2017-01-24 | 2017-05-24 | 中国科学院昆明植物研究所 | Piper magen essential oil and extraction and chemical component analysis method thereof |
-
2019
- 2019-04-09 CN CN201910282006.5A patent/CN109966348A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701321A (en) * | 2017-01-24 | 2017-05-24 | 中国科学院昆明植物研究所 | Piper magen essential oil and extraction and chemical component analysis method thereof |
Non-Patent Citations (8)
| Title |
|---|
| 孙协军等: "乙醇体积分数对山楂提取物黄酮组成及抗氧化活性的影响", 《中国食品学报》 * |
| 幸宏伟: "玫瑰花醇提取物对油脂的抗氧化作用", 《重庆工商大学学报(自然科学版)》 * |
| 张水平等: "胡椒叶抗氧化物质提取条件初探", 《热带作物学报》 * |
| 张水平等: "胡椒叶抗氧化能力分析及其活性成分分离鉴定", 《现代食品科技》 * |
| 张焱珍等: "77种植物提取物对植物病原菌的生长抑制作用", 《天然产物研究与开发》 * |
| 新华网: "中科院研究人员在云南发现胡椒新种", 《食品工业》 * |
| 王瑞婷: "黄芩茎叶总黄酮对Aβ致大鼠学习记忆损伤的影响及机制研究", 《中国博士学位论文全文数据库-医药卫生科技辑》 * |
| 邢旭等: "胡椒油的研究进展", 《农业工程技术.农产品加工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111718799A (en) * | 2020-06-30 | 2020-09-29 | 三沙南海美源岛生物科技有限公司 | Method for extracting pepper leaf essential oil by eutectic solvent method and ultrasonic-assisted distillation |
| CN111718798A (en) * | 2020-06-30 | 2020-09-29 | 三沙南海美源岛生物科技有限公司 | Method for extracting pepper leaf essential oil by microwave-assisted distillation through eutectic solvent method |
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