CN109966335A - A kind of antineoplastic combinations - Google Patents

A kind of antineoplastic combinations Download PDF

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CN109966335A
CN109966335A CN201910301589.1A CN201910301589A CN109966335A CN 109966335 A CN109966335 A CN 109966335A CN 201910301589 A CN201910301589 A CN 201910301589A CN 109966335 A CN109966335 A CN 109966335A
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herba epimedii
semen momordicae
chinese medicine
medicine composition
composition according
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CN109966335B (en
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朱晓新
王娅杰
屠鹏飞
杨庆
李琦
李玉洁
翁小刚
陈颖
蔡维艳
阚月一
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Institute of Materia Medica of CAMS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
    • A61K36/296—Epimedium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/42—Cucurbitaceae (Cucumber family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH

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Abstract

The present invention relates to a kind of antineoplastic combinations, the composition is by semen momordicae and Herba Epimedii as Chinese medicine material medicine, it is prepared by processing, wherein semen momordicae and Herba Epimedii are fed intake using crude drug, the two weight ratio is 1-10:1-10, and preferably 1-5:1-5 more selects 3:25,1:1,2:1 and 1:2, most preferably 1:1, the present invention apply Chinese medicine semen momordicae with Herba Epimedii jointly, in rationally control dosage, while retaining effective component, focuses on compatibility attenuation, mitigate its side effect, enhance body's immunity simultaneously, reaches better antitumous effect.

Description

A kind of antineoplastic combinations
Technical field:
The present invention relates to a kind of Chinese medicine composition, in particular to the combination of a kind of semen momordicae and Herba Epimedii use in conjunction.
Background technique:
Chinese medicine is widely used in anti-tumor aspect, more clearly with the Chinese traditional medicine composition of antitumor action.This hair It is bright on the basis of existing technology, the new Chinese traditional medicine composition with antitumor action of one kind of invention.
Semen momordicae (scientific name: Momordica cochinchinensis (Lour.) Spreng.), also known as: vomiting nut, glutinous meal Fruit, mouse draw wax gourd etc..Perennial herbaceous stem rattan wood, there is the effect of dispersing swelling and dissipating binds, toxic removing.Nature and flavor: bitter, micro-sweet, it is cool；It is toxic.Return Through: return liver, spleen, stomach meridian.Major function: mass dissipating and swelling eliminating, attacks poison and treats sore.For sore swollen toxin, acute mastitis, scrofula, anal fistula, chronic eczema, The favus of the scalp.Usage and dosage: 0.9-1.2g；Suitable amount used externally is ground, and applies affected part with oil or vinegar tune.Contain momordic acid in semen momordicae (momordic acid), gypsogenin (gypsogenin), oleanolic acid (oleanolic acid), alpha-eleostearic acid (α- Elaeostearic acid), gas base acid, sterol.Modern pharmacological studies have shown that semen momordicae, which is used as, attacks poison hypoglycemic, anti- Bacterium, anticancer, analgesia etc. have certain effect.But because its toxicity is big, clinical application is restricted, and it is used as in few commonly use Medicine, since ancient times, more external applications are rarely used in for oral administration.
Herba Epimedii (scientific name: Epimedium brevicornu Maxim.) herbaceos perennial, Herba Epimedii herb is for medicine With.Cure mainly impotence and premature ejaculation, pain in low back and legs, numb limb, hemiplegia, neurasthenia, amnesia, tinnitus, dizzy equal diseases.Herba Epimedii Acrid-sweet flavor is warm-natured, walks liver kidney two warp.For mend the gate of vitality, strengthening the essence gas, strengthening the bones and muscles, tonifying kidney and strengthening yang key medicine, clinic be usually used in treatment male The diseases such as sub- impotence do not lift, spermatorrhoea premature ejaculation, urinary incontinence and female sterility.Herba Epimedii contains icariine, volatile oil, ceryl alcohol, plant The ingredients such as object sterol, tannin, vitamin E.The excited sexual performance of energy, has animal and promotes gonopoiesis effect.There are also decompressions (to cause Peripheral vasodilation), hypoglycemic, diuresis, kobadrin and vitamin E sample effect.Pharmacological experiment study shows Herba Epimedii energy Increase cardiovascular and cerebrovascular blood flow, promote hematopoiesis function, immune function and bone metabolism, there is anti-aging, antitumor and other effects.
Herba Epimedii is as traditional tonic medicine, and multi-pass crosses enhancing immunity of organism function to reach antitumor effect in modern clinic practice Fruit.
When the present invention is with malicious class Chinese medicine treatment malignant tumour is attacked, Chang Peiwu qi-restoratives medicine, drug for invigorating blood circulation and eliminating stasis and antipyretic etc. To improve the principle of therapeutic effect, gas consumption easy for malignant tumour hurts saliva, can cause gas with treatment means such as chemotherapy, radiotherapies in addition Yin it is more empty, using attack malicious drug matching qi-restoratives medicine can building up healthful vital energy, help is precise and tiny, drive heresy anticancer.Attack poison compatibility qi-restoratives medicine Meet the attacking pathogenic factor with poisonous drug that " Synopsis Golden Chamber " is proposed, preferably protect in the combination principle of Gu Zhengqi.
For this purpose, the present invention by existing Chinese medicine semen momordicae and Herba Epimedii combine, invent a kind of combination treatment, by two medicines close With mutually collaboration, eliminating pathogen to support vital qi are then antitumor.
The present invention applies Chinese medicine semen momordicae with Herba Epimedii jointly, in rationally control dosage, retains effective component Meanwhile focusing on compatibility attenuation, mitigate its side effect, while enhancing body's immunity, reaches better antitumous effect.
Summary of the invention:
The present invention provides a kind of Chinese medicine composition, and the composition is by semen momordicae and Herba Epimedii as Chinese medicine material medicine, warp Processing is crossed to be prepared.
Composition of the present invention, wherein semen momordicae and Herba Epimedii are fed intake using crude drug, and the two weight ratio is 1-10: 1-10, preferably 1-5:1-5 more select 3:25,1:1,2:1 and 1:2, most preferably 1:1.
It is living to obtain Chinese medicine by the way that semen momordicae and Herba Epimedii are simultaneously or separately extracted processing for composition of the present invention Property substance, then the active material is prepared into pharmaceutical preparations composition by conventional pharmaceutics routine techniques.
It is of the present invention that semen momordicae and Herba Epimedii are simultaneously or separately extracted into processing, the method is as follows:
Semen momordicae crushes, and is extracted respectively with 95% second alcohol and water, extracting solution heating and refluxing extraction 2 times, each 2h, closes And extracting solution, filter paper filtering rotate extracting solution.
Herba Epimedii is respectively adopted 95% second alcohol and water and directly extracts, extracting solution filtering after rotate, wherein water extract into Row freeze-drying process, alcohol extract are dry by vacuum oven.
The present invention respectively obtains semen momordicae alcohol extracting by Different Extraction Method, semen momordicae water mentions, Herba Epimedii alcohol extracting, Herba Epimedii Water mentions four kinds of extracts
Four kinds of extracts can have following different combination:
Combine 1 semen momordicae alcohol extracting, Herba Epimedii alcohol extracting
Combine that 2 semen momordicae water mention, Herba Epimedii water mentions
Combine 3 semen momordicae alcohol extractings, Herba Epimedii water mentions
Combine 4 semen momordicae water mention, Herba Epimedii alcohol extracting
The present invention is most preferred, is combination 1 semen momordicae alcohol extracting, Herba Epimedii alcohol extracting, naturally it is also possible to semen momordicae and Herba Epimedii It is extracted jointly after mixing with ethyl alcohol and obtains mixed extract.
Drug extracting method of the invention can be with the following method:
Semen momordicae health product pulverizer crushes, and crosses No. 10 sieves, is extracted respectively with 95% second alcohol and water, extracting solution heats back Stream extracts 2 times, each 2h, combined extract, and filter paper filtering rotates extracting solution.
Herba Epimedii health product is respectively adopted 95% second alcohol and water and directly extracts, the same semen momordicae of extracting method.After revolving, water Extract carries out freeze-drying process, and alcohol extract is dry for 24 hours by vacuum oven.Momordica cochinchiensis is respectively obtained by Different Extraction Method Sub- alcohol extracting, semen momordicae water mention, Herba Epimedii alcohol extracting, Herba Epimedii water mention four kinds of extracts,
The present invention is prepared into Chinese materia medica preparation combination using the extract of said extracted as active ingredient of Chinese herbs after processing Object.
Chinese materia medica preparation composition of the invention, can be added pharmaceutically acceptable carrier, wherein active Chinese drug component as needed Shared weight percent can be 0.1-99.9% to ingredient in the formulation, remaining is pharmaceutically acceptable carrier.In of the invention Medicine preparation compositions can exist in a unit, and the unit dosage form refers to the unit of preparation, every such as tablet Piece, every capsule of capsule, every bottle of oral solution, every bag of granule etc..
Chinese materia medica preparation composition of the invention can be any pharmaceutical dosage form, these dosage forms include: tablet, sugar coated tablet Agent, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral solution, mouth containing agent, granule, electuary, Pill, powder, paste, sublimed preparation, suspension, pulvis, solution, injection, suppository, ointment, emplastrum, creme, spray, Drops, patch.Preparation of the invention, preferably peroral dosage form, such as: capsule, oral solution, granule, pill, dissipates tablet Agent, sublimed preparation, paste etc..Most preferably tablet.
Chinese materia medica preparation composition of the invention, oral administration preparation can contain common excipient, such as adhesive, Filler, diluent, tablet agent, lubricant, disintegrating agent, colorant, flavoring agent and wetting agent when necessary can wrap tablet Clothing.
Applicable filler includes cellulose, mannitol, lactose and other similar fillers.Suitable disintegrating agent packet Include starch, polyvinylpyrrolidone and starch derivatives, such as sodium starch glycollate.Suitable lubricant includes, such as firmly Fatty acid magnesium.Suitable pharmaceutically acceptable wetting agent includes lauryl sodium sulfate.
Can be by mixing, filling, commonly method prepares solid oral composition for tabletting etc..Carrying out mixing repeatedly can make to live Property substance be distributed in entirely using in those of a large amount of fillers composition.The form of oral liquid for example can be aqueous Or oily suspensions, solution, emulsion, syrup or elixir, or can be and a kind of can use water or other suitable before use The dry products of carrier compounding.This liquid preparation is containing conventional additive, such as suspending agent, for example, sorbierite, syrup, Methylcellulose, gelatin, hydroxyethyl cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifier, Such as lecithin, anhydro sorbitol monooleate or Arabic gum；Non-aqueous carrier (they may include edible oil), such as apricot Benevolence oil, fractionated coconut oil, the oily ester of the ester of such as glycerol, propylene glycol or ethyl alcohol；Preservative, such as para hydroxybenzene methyl esters or right Nipasol or sorbic acid, and if desired, contain conventional flavouring agent or colorant.
For injection, the fluid unit dosage form of preparation contains active material and sterile carrier of the invention.According to carrier And concentration, this compound can be suspended or be dissolved.The preparation of solution is usually by the way that active material is dissolved in a kind of load In body, disinfection is filtered before being loaded into a kind of suitable bottle or ampoule, is then sealed.For example a kind of local anaesthesia of auxiliary material Agent, preservative and buffer are also soluble in this carrier.It, can be after being packed into bottle by this in order to improve its stability Kind composition frost, and under vacuum remove water.
Chinese materia medica preparation composition of the invention is optionally added suitable pharmaceutically acceptable when being prepared into medicament Carrier, the pharmaceutically acceptable carrier are selected from: mannitol, sorbierite, sodium pyrosulfite, sodium hydrogensulfite, sodium thiosulfate, Cysteine hydrochloride, thioacetic acid, methionine, injection Vitamin B_6 DTA disodium, Ethylenediaminetetraacetic Acid Calcium Salt, carbonate, the vinegar of monovalence alkali metal Hydrochlorate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, Maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and Its derivative, alginates, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surface-active Agent, polyethylene glycol, cyclodextrin, beta-cyclodextrin, phospholipid material, kaolin, talcum powder, calcium stearate, magnesium stearate etc..
Preparation of the invention determines usage and dosage according to the patient's condition when in use, can often taken three times per day, each 1-20 Agent, such as: 1-20 bags or grain or piece.
Beneficial effects of the present invention are further illustrated below by way of experimental data:
Effect experiment of the present invention uses the semen momordicae of embodiment 1, Herba Epimedii with 1:1 ratio alcohol extracting mixture.
The present invention is used alone when semen momordicae, Herba Epimedii are shared with 1:1 ratio compared with two medicines by optimal screening, discovery, There is more significant inhibitory effect to tumour cell, and after two medicines share, the influence to normal cell LO2 is smaller, thus pushes away Surveying two medicines and sharing has the function of attenuation synergistic.
Experimental data is as follows:
Experiment 1. carries out the research of semen momordicae, Herba Epimedii anti-tumor activity by MTT colorimetric method, to filter out effective conjunction It is composed with ratio and tumor suppression.And by the drug effect of different proportion in normal liver cell LO2.Different proportion is observed by mtt assay The influence that drug is proliferated LO2.
Related data are as follows:
1 semen momordicae of table, Herba Epimedii list are decocted and are shared to A549 cell 72h inhibiting rate
* p < 0.05 compared with blank control has statistical difference；■ alcohol extracting p < 0.05 compared with water mentions has statistics Learn difference；★ is mono- to decoct p < 0.05 compared with mixed decoct, and has statistical difference；◇ is mono- to decoct p < 0.05 compared with compatibility, has statistics Learn difference.
2 semen momordicae of table, Herba Epimedii list are decocted and are shared to Bel7402 cell 72h inhibiting rate
* p < 0.05 compared with blank control has statistical difference；■ alcohol extracting p < 0.05 compared with water mentions has statistics Learn difference；★ is mono- to decoct p < 0.05 compared with mixed decoct, and has statistical difference；◇ is mono- to decoct p < 0.05 compared with compatibility, has statistics Learn difference.
3 semen momordicae of table, Herba Epimedii list are decocted and are shared to SK-OV-3 cell 72h inhibiting rate
* p < 0.05 compared with blank control has statistical difference；■ alcohol extracting p < 0.05 compared with water mentions has statistics Learn difference；★ is mono- to decoct p < 0.05 compared with mixed decoct, and has statistical difference；◇ is mono- to decoct p < 0.05 compared with compatibility, has statistics Learn difference.
4 semen momordicae of table, Herba Epimedii list are decocted and are shared to HT-29 cell 72h inhibiting rate
* p < 0.05 compared with blank control has statistical difference；■ alcohol extracting p < 0.05 compared with water mentions has statistics Learn difference；★ is mono- to decoct p < 0.05 compared with mixed decoct, and has statistical difference；◇ is mono- to decoct p < 0.05 compared with compatibility, has statistics Learn difference.
5 semen momordicae of table, Herba Epimedii list are decocted and are shared to MGC803 cell 72h inhibiting rate
* p < 0.05 compared with blank control has statistical difference；■ alcohol extracting p < 0.05 compared with water mentions has statistics Learn difference；★ is mono- to decoct p < 0.05 compared with mixed decoct, and has statistical difference；◇ is mono- to decoct p < 0.05 compared with compatibility, has statistics Learn difference.
Two medicines have certain Inhibit proliferaton to act on different human tumor cells as seen from the above table, by comparing different groups Other IC50, it is possible to find when semen momordicae and Herba Epimedii are applied in combination with the ratio of 3:25, the proliferation inhibition rate of cell is higher than medicine Proliferation inhibition rate when object is applied alone.For the optimal proportion that two medicine of further screening shares, we expand two medicines and share ratio sieve Range is selected, the drug that different proportion combines is respectively applied to the obvious A549 cell of function and effect and MGC803 cell.Knot Fruit is as shown in table 6.
6 Herba Epimedii alcohol of table refers to that two medicine differences share ratio to A549 and MGC803 cell 72h inhibiting rate
* p < 0.05 compared with blank control has statistical difference
As shown in Table 6 when two medicines share ratio be 3:25 and 1:1 when it is more significant to the proliferation inhibiting effect of tumour cell.For Further relatively influence of two different proportions to cell, the semen momordicae that 3:25 and 1:1 is matched, that Herba Epimedii acts on normal hepatocytes is thin Born of the same parents 72h, the semen momordicae for observing different ratio, Herba Epimedii are to the toxicity of normal cell, and the results are shown in Table 7.
7 Herba Epimedii alcohol of table refers to that two medicine differences share ratio to the inhibiting rate of LO2 cell
When table 7 shows Herba Epimedii alcohol extracting or semen momordicae, Herba Epimedii compatibility shares in varing proportions, normal liver cell is produced Raw slight inhibiting effect.And compare, weaker on normal liver cell influence when drug is with 1:1 ratio compatibility, cell is even There is negative growth trend.Thus initial guess has the function of attenuation synergistic when two medicines are shared with 1:1 ratio.
Experiment 2. further verifies two medicine collective effects to the synergy of A549 with Chou-Talalay association index method Attenuation.Association index (combination indices, CIs) and dosage by calculating semen momordicae and Herba Epimedii reduce Index (dose-reduction index, DRI), finally defining two medicines and sharing ratio is 1:1.
Related the result is shown in Figure 1-Fig. 4.
Experiment 3. is the influence of more intuitive observation drug cell proliferation ability, detects two medicines and is shared with 1:1 ratio Afterwards, the plate clone Forming ability after A549 cell, and, knot for statistical analysis with factor analysis method are acted on various concentration Fruit shows, relative to blank control group cell, the plate clone Forming ability of dosing group cell is substantially reduced, with blank control group (P < 0.001) statistically significant compared to difference.
Related result is shown in Fig. 5, Fig. 6.
Test and construct Lewis lung cancer model in 4. bodies, the antitumous effect excessive to wood is verified, as a result with experiment in vitro Consistent, two medicines can significantly inhibit Lewis subcutaneous transplantation tumor proliferation after sharing.
Related result is shown in Fig. 7
8 different dosing group tumor quality of table and tumor control rate compare (n=10)
Influence (n=10) of 9 drug of table to mouse immune internal organs
Experiment 5. detects IFN-γ and IL-4 expression quantity in administration each group mice serum by ELISA, it is believed that wood is excessive total Same-action can enhance the autoimmune function of Lewis lung cancer in mice.Related result is shown in Fig. 8:
Detailed description of the invention:
Fig. 1 difference shares proportional action concentration and inhibiting rate relationship when A549
Fig. 2 difference shares proportional action in the CI coefficient and inhibiting rate relationship of A549
Two medicine 3:25 proportional action of Fig. 3 inhibiting rate and DRI value relationship when A549
Two medicine 1:1 proportional action of Fig. 4 inhibiting rate and DRI value relationship when A549
A549 cell plates cloning capacity when two medicine of Fig. 5 shares
Fig. 6 various concentration drug effect formed after A549 clone number compare * represent two medicines share it is poor compared with the control group Different significant (P < 0.01)
Fig. 7 difference group tumor volume change (P > 0.05)
Fig. 8 administration middle dose group and blank control group IFN-γ, IL-4 level compare
Fig. 9 semen momordicae Herba Epimedii extraction process figure
Specific embodiment:
The present invention is further illustrated by the following examples, but not as limitation of the present invention.
Embodiment 1
Semen momordicae health product pulverizer crushes, and crosses No. 10 sieves, is extracted respectively with 95% ethyl alcohol, heating and refluxing extraction 2 times, Each 2h, combined extract, filter paper filtering are evaporated off ethyl alcohol and obtain extract.
Herba Epimedii health product is extracted using 95% ethyl alcohol, and heating and refluxing extraction 2 times, each 2h, combined extract, filter paper Filtering, is evaporated off ethyl alcohol and obtains extract.
Two kinds of extracts mix up to pharmaceutically active substance of the present invention.
Embodiment 2
Semen momordicae health product, Herba Epimedii health product are extracted using 95% ethyl alcohol, heating and refluxing extraction 2 times, each 2h, are merged Extracting solution, filter paper filtering, is evaporated off ethyl alcohol and obtains the mixed extract of two kinds of drugs.
Embodiment 3
Semen momordicae health product pulverizer crushes, and crosses No. 10 sieves, is extracted with water, extracting solution heating and refluxing extraction 2 times, every time 2h, combined extract, filter paper filtering rotate extracting solution.
Herba Epimedii health product is directly extracted using water, the same semen momordicae of extracting method.After revolving, it is dry that water extract carries out freezing Dry processing, alcohol extract are dry for 24 hours by vacuum oven.
Two kinds of extracts mix up to pharmaceutically active substance of the present invention.
Embodiment 4
Semen momordicae health product, Herba Epimedii health product are directly extracted using water, and extracting solution heating and refluxing extraction 2 times, each 2h, Combined extract, filter paper filtering, revolving extracting solution obtain the mixed extract of two kinds of drugs.
Embodiment 5
The mixed extract for taking 5g embodiment 1 is uniformly mixed with 15g starch, and povidone is added as adhesive, granulation pressure 100, tablet is made in piece.
6 injection of embodiment
2 extract of 5g embodiment and glucose 4.5g, sodium thiosulfate 0.9g and distilled water 1ml are taken, said components are mixed Close uniformly after, filtering, sterilizing packing 100 to get.

Claims (9)

1. a kind of Chinese medicine composition, the composition by semen momordicae and Herba Epimedii as Chinese medicine material medicine, by processing preparation At.
2. Chinese medicine composition according to claim 1, wherein semen momordicae and Herba Epimedii are fed intake using crude drug, the two weight ratio Example is 1-10:1-10.
3. Chinese medicine composition according to claim 1, wherein semen momordicae and Herba Epimedii are fed intake using crude drug, the two weight ratio Example is 1-5:1-5.
4. Chinese medicine composition according to claim 1, wherein semen momordicae and Herba Epimedii are fed intake using crude drug, the two weight ratio Example is 3:25,1:1,2:1 and 1:2.
5. Chinese medicine composition according to claim 1, wherein semen momordicae and Herba Epimedii are fed intake using crude drug, the two weight ratio Example is 1:1.
6. Chinese medicine composition according to claim 1, be by the way that semen momordicae and Herba Epimedii are simultaneously or separately extracted processing, Active Chinese drug component substance is obtained, then the active material is prepared into pharmaceutical preparation group by conventional pharmaceutics routine techniques Close object.
7. Chinese medicine composition according to claim 1 respectively obtains semen momordicae alcohol extracting, semen momordicae by Different Extraction Method Water mentions, Herba Epimedii alcohol extracting, Herba Epimedii water mentions four kinds of extracts, four kinds of extracts following different combination:
Combine 1 semen momordicae alcohol extracting, Herba Epimedii alcohol extracting
Combine that 2 semen momordicae water mention, Herba Epimedii water mentions
Combine 3 semen momordicae alcohol extractings, Herba Epimedii water mentions
Combine 4 semen momordicae water mention, Herba Epimedii alcohol extracting.
8. Chinese medicine composition according to claim 1 is most preferably combination 1 semen momordicae alcohol extracting, Herba Epimedii alcohol extracting, or It is extracted jointly after semen momordicae and Herba Epimedii mixing with ethyl alcohol and obtains mixed extract.
9. pharmaceutically acceptable carrier can be added, wherein Chinese medicine in Chinese medicine composition according to claim 1 as needed Shared weight percent can be 0.1-99.9% to active constituent in the formulation, remaining is pharmaceutically acceptable carrier.
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* Cited by examiner, † Cited by third party
Title
尹剑云等: "淫羊藿及其有效成分抗肿瘤机制的研究进展", 《中西医结合学报》 *

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