CN109943470B - Cord blood stem cell collector - Google Patents
Cord blood stem cell collector Download PDFInfo
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- CN109943470B CN109943470B CN201910232638.0A CN201910232638A CN109943470B CN 109943470 B CN109943470 B CN 109943470B CN 201910232638 A CN201910232638 A CN 201910232638A CN 109943470 B CN109943470 B CN 109943470B
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 114
- 210000004700 fetal blood Anatomy 0.000 title claims abstract description 57
- 238000005192 partition Methods 0.000 claims abstract description 114
- 210000004369 blood Anatomy 0.000 claims abstract description 10
- 239000008280 blood Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 230000008569 process Effects 0.000 claims abstract description 8
- 238000005070 sampling Methods 0.000 claims description 31
- 238000012545 processing Methods 0.000 claims description 16
- 238000000926 separation method Methods 0.000 claims description 15
- 238000001514 detection method Methods 0.000 claims description 10
- 230000002159 abnormal effect Effects 0.000 claims description 9
- 238000012795 verification Methods 0.000 claims description 9
- 239000000853 adhesive Substances 0.000 claims description 8
- 230000001070 adhesive effect Effects 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 239000000741 silica gel Substances 0.000 claims description 6
- 229910002027 silica gel Inorganic materials 0.000 claims description 6
- 230000006835 compression Effects 0.000 claims description 5
- 238000007906 compression Methods 0.000 claims description 5
- 238000004891 communication Methods 0.000 claims description 4
- 239000003146 anticoagulant agent Substances 0.000 claims description 3
- 229940127219 anticoagulant drug Drugs 0.000 claims description 3
- 230000006378 damage Effects 0.000 claims description 3
- 210000003743 erythrocyte Anatomy 0.000 claims description 3
- 210000004180 plasmocyte Anatomy 0.000 claims description 3
- 238000011835 investigation Methods 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 230000001276 controlling effect Effects 0.000 description 8
- 239000007788 liquid Substances 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
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- 230000006872 improvement Effects 0.000 description 3
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Abstract
The invention discloses an cord blood stem cell collector which comprises a blood taking needle, a main bag, an auxiliary bag, a first communicating pipe and a second communicating pipe, wherein the main bag is communicated with the auxiliary bag through the first communicating pipe, the first communicating pipe is also provided with a first one-way valve, the blood taking needle is connected with the main bag through the second communicating pipe, the first communicating pipe is provided with a second one-way valve, the main bag is internally provided with a movable partition structure, the movable partition structure can partition the main bag to divide the main bag into a left part and a right part, the upper side of the main bag is provided with at least one graduated tube, and the lower side of the main bag is provided with a one-way communicating pipe. The invention can rapidly collect and store the stem cells, can analyze the state of the stem cells in the storage process in time, performs backup storage analysis, and reduces the risk of deviation of the collected stem cells.
Description
Technical Field
The invention belongs to the technical field of medical treatment, and particularly relates to a cord blood stem cell collector.
Background
Cord blood stem cells are important personal biological resources which are reserved only once for a life of a child, cord blood stored in the child is equal to one life backup of the child, and the cord blood can effectively treat dozens of intractable diseases and various incurable diseases at present, and the types of diseases which can be treated by the cord blood stem cells are continuously increased. At present, with the increasing of the effect of cord blood stem cells, especially for the improvement of the importance of saving lives, the economic level of people is continuously improved, and the consciousness of storing cord blood stem cells is also continuously improved, however, most of the current storage aiming at the cord blood stem cells is stored through a single storage bag, and because the number of the cord blood stem cells is less, the cord blood stem cells cannot be separately extracted and stored in a plurality of positions, and once the pollution of the stem cells occurs, the loss is heavy and the cord blood stem cells cannot be recovered; moreover, the stem cells need to be collected periodically due to long storage time, and when the collected indexes have deviation, the existing technology cannot well verify the indexes.
In consideration of the importance and uniqueness of the cord blood stem cells, important preservation needs to be performed, how to verify the collected information, how to perform important backup under a limited number, and reducing risks caused by stem cell damage, which is a difficult point that the cord blood stem cell collection needs to be focused in the future.
Disclosure of Invention
The invention can rapidly collect and separate stem cells, improve the stability of the stem cells in later storage, rapidly back up and store the stem cells by different temperature control, and reduce the risk of stem cell pollution.
The application discloses cord blood stem cell collector, including blood taking needle, main bag, vice bag, first communicating pipe, second communicating pipe, communicate through first communicating pipe between main bag and the vice bag, still be provided with first check valve on the first communicating pipe, the blood taking needle pass through second communicating pipe with the main bag is connected, be provided with the second check valve on the first communicating pipe, be provided with the activity in the main bag and cut off the structure, the activity cuts off the structure and can cut off the main bag, will the main bag is cut apart into left part and right part, main bag upside is provided with at least one scale mark pipe, main bag downside one-way conduction pipe.
The cord blood stem cell collector, the graduated flask is the cuboid cylinder shape, and its surface is provided with the scale, and its inboard sets up a plurality of check pressure valves along length direction, a plurality of check pressure valves switch on according to pressure from bottom to top, the graduated flask passes through a plurality of spaces are cut apart into to a plurality of check valves, and every space outside is provided with the sampling and detects the mouth.
The cord blood stem cell collector is characterized in that the movable partition structure is arranged in the middle of the main bag, the main bag can be arranged into a left part and a right part, the movable partition result comprises a first partition structure and a second partition structure, and when the partition is performed, the first partition structure is firstly subjected to partition operation, and then the second partition structure is subjected to partition operation; the first partition structure comprises an upper part and a lower part, the upper part and the lower part are respectively arranged on the inner surfaces of the front surface and the rear surface of the main bag, and the lower part comprises an arc with an opening, a first hook outside the arc with the opening and a first vertical baffle outside the first hook; the upper part comprises a round bar matched with the arc with the opening, a second hook coupled with the first hook and a second vertical baffle outside the second hook;
the second partition structure includes a pressure-bondable portion including a first width portion and a second width portion, the second width portion being larger than the first width portion, the second width portion being outside the first width portion, and a thermal adhesive seal member being provided between the first width portion and the second width portion.
When the umbilical cord blood stem cell collector is used for separating, firstly, the first separation structure is subjected to separation operation, and then, the second separation structure is subjected to separation operation, so that the method specifically comprises the following steps: applying a first external pressure to the first partition structure, pressing the first partition structure to divide the main bag into left and right two partitioned portions, applying a second external pressure to the first width portion of the second partition structure, applying a third external pressure to the second width portion, and applying a predetermined amount of heat to the thermal adhesive seal to completely partition the main bag.
In the cord blood stem cell collector and the sealing process of the second partition structure, the internal space of the main bag is compressed, and stem cells in the main bag are compressed into the at least one graduated tube.
The cord blood stem cell collector, the unidirectional flux tube outside is provided with the silica gel cap, the silica gel cap can puncture repeatedly.
The cord blood stem cell collector, still be provided with the rotating head on the first connecting pipe, the rotating head is in when the main bag carries out the centrifugation, can control the rotating head rotates.
The umbilical cord blood stem cell collector is characterized in that a temperature control box is further arranged on the outer side of the main bag, and a press machine, a rotating motor, a puncture needle, temperature control equipment, a controller, a sampling interface and an output interface are arranged in the temperature control box; the press is used for applying pressure to the movable partition structure so as to partition the main bag; the rotating motor is used for being connected with the rotating head and controlling the main bag to rotate and centrifuge; the puncture needle is used for puncture sampling of the one-way conduction tube and the graduated tube; the temperature control equipment is used for controlling the temperature of the temperature control box, and the temperature control equipment at least comprises two temperature control equipment, is arranged on the left side and the right side of the temperature control box respectively and is used for controlling the temperature of the left part and the right part of the main bag respectively; the sampling interface is used for connecting the sampling needle through the second communicating pipe; the output interface is used for being connected with the auxiliary bag through the first communication pipe; the controller is used for controlling the press machine, the rotating motor, the puncture needle and the temperature control equipment.
The umbilical cord blood stem cell collector, the temperature control box also comprises a first temperature sensor and a second temperature sensor, and the controller comprises a first comparison unit and a processing unit; the temperature control device comprises a first temperature sensor, a second temperature sensor, a first comparison unit, a processing unit, a standby temperature control unit and a standby temperature control unit, wherein the first temperature sensor is used for collecting a first temperature on the left side in a temperature control box, the second temperature sensor is used for collecting a second temperature on the right side in the temperature control box, the first temperature and the second temperature are both sent to the first comparison unit in a controller, the first comparison unit compares the first temperature with the second temperature and then obtains a first temperature difference value, if the first temperature difference value exceeds a first temperature threshold value, a first early warning temperature signal is sent to the processing unit, the processing unit checks a temperature to start the standby temperature control unit and checks abnormal reasons, records the abnormal reasons, and after the abnormal reasons are checked, the standby temperature control unit is closed.
The cord blood stem cell collector collects cord blood samples through the sampling needle and conveys the cord blood samples to the main bag; the controller controls the rotating motor in the temperature control box to rotate, so that cord blood is fully and uniformly mixed with the anticoagulant in the main bag, after the preset time is waited, the rotating motor is controlled again to rotate and centrifuge, the first one-way valve is opened, and centrifuged plasma and red blood cells after stem cell separation are conveyed into the auxiliary bag through the first communicating pipe; the controller controls the movable partition structure to set the main bag into a left part and a right part, when the partition is executed, the press is controlled to apply pressure to the first partition structure to conduct partition operation, then the second partition structure is controlled to conduct partition operation, the second partition structure is controlled to conduct partition, meanwhile, the plurality of one-way pressure valves in the graduated tube are controlled to be opened, one part of stem cells are compressed into the graduated tube, and meanwhile, the temperature control box is divided into two independent temperature control spaces according to the fact that the movable partition structure is a dividing point;
during the storage period of the stem cells, the stem cells in at least one of the spaces in the graduated tube in the space on the left side of the temperature control box are collected at regular time from the sampling detection port through a puncture needle, and a first parameter is obtained; the controller judges the index state of the stem cells according to the first parameter, if the index state has deviation, the puncture needle punctures the one-way conduction pipe in the left space of the temperature control box to collect the stem cells, a second parameter is obtained, and the index state of the second parameter and the index state of the first parameter are compared;
if the second parameter is the same as the first parameter index, puncturing the one-way conduction pipe in the right space of the temperature control box to collect stem cells to obtain a third parameter, and comparing the third parameter with the first parameter and the second parameter; if the third parameter is different from the first parameter and the second parameter, the stem cells in the space on the left side of the temperature control box are independently extracted for manual detection to judge the reason of the deviation; if the third parameter is the same as the first parameter and the second parameter, extracting a sample to manually analyze the state of the stem cells;
if the second parameter is different from the first parameter index, whether the second parameter accords with the index state is judged, if so, stem cells in at least one of the spaces in the graduated tube in the space on the left side of the temperature control box are detected once again to obtain a first verification parameter, if the first verification parameter is the same as the second parameter index, the index is normal, if the first verification parameter is different from the second parameter index, the damage of the stem cells in the graduated tube is recorded, and the next sampling is directly carried out from the one-way conduction tube in the space on the left side of the temperature control box.
The beneficial effects of the invention include: 1. the cord blood stem cells are collected, separated and stored integrally, so that cord blood is conveniently and quickly collected; 2. the temperature can be regulated and controlled independently, and the periodic sampling can be carried out, so that the regular and accurate analysis and comparison in the preservation process are facilitated; 3. the processed blood plasma is quickly separated and integrally separated, so that the cost is saved, the separate separation processing in the backup process is reduced, and the separated stem cells are respectively stored through the movable partition structure after the integral separation processing, thereby being beneficial to reducing the stem cell pollution caused by improper storage, improving the stability of the stored stem cells and reducing the risk; 4. one of the main improvement points of the invention is that the first partition part and the second partition part are used for different partitions, different isolation modes such as physical and thermal compression are used for structural partition, the sealing performance of the partitions is improved, the second partition part is combined with the space in the compression main bag, stem cells are pressed into the graduated tube in a matched manner, double functions are realized through one part, and the practicability of the part is improved. 5. The invention has the advantages that the controller can be used for carrying out sampling comparison on a plurality of regular divided spaces, the graduated tube, the left main bag and the right main bag are respectively used for carrying out sampling comparison to judge the storage state of the stem cells, accurate storage results are obtained through analysis and judgment, the deviation of detection results caused by inaccurate sampling or inaccurate samples in the graduated tube is avoided, meanwhile, the pollution of certain part of stem cells caused by the deviation of the temperature control devices can be prevented through different temperature control devices in the left temperature control space and the right temperature control space, and the storage stability of the stem cells is improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the cord blood stem cell collector of the present invention.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
The invention can rapidly collect and separate stem cells, improve the stability of the stem cells in later storage, rapidly back up and store the stem cells by different temperature control, and reduce the risk of stem cell pollution.
Fig. 1 is a schematic diagram showing the overall structure of the cord blood stem cell collector of the present invention. The blood taking needle comprises a blood taking needle 1, a second communicating pipe 2, a right-side graduated tube 3, a second communicating pipe input port 4, a first communicating pipe output port 5, a rotating head 6, a first communicating pipe 7, a one-way valve 8, a controller box 9, a temperature control box 10, a main bag 11, a left-side one-way conduction pipe 12, a second width boundary line 13, a first partition structure 14, a temperature control box space partition part 15, a right-side one-way conduction pipe 16, a thermal bonding sealing part 17, an auxiliary bag 18 and a left graduated tube 19. The controller box 9 and the temperature control box 10 are designed integrally, the controller box 9 and the temperature control box 10 are separated through a heat insulation material, and the controller box 9 is shielded in a shielding mode except for necessary connection control lines.
Preferably, still be provided with at least one deck inner bag in the temperature control box 10, the inner bag parcel main bag and graduated tube etc. keep there being the part of stem cell, through the inner bag will main bag and rotating head, temperature controller part divide apart, the inner bag is provided with temperature regulation's opening, be provided with compressive material on the inner bag, prevent giving exerting pressure damage during the main bag, the inner bag can pass through opening input liquid nitrogen keeps the stem cell to be in the save temperature of regulation through liquid nitrogen, the inner bag still reserves the bulge of one-way conduction pipe, and when needs draw the stem cell through one-way conduction pipe, the breach that the pjncture needle can penetrate is opened to the bulge.
The thermal adhesive sealant 17 is defined as a boundary between the first width and the second width, a width between the thermal adhesive sealant 17 and the second width boundary 13 is defined as the second width, and a width between the thermal adhesive sealant 17 and the boundary of the first partition structure 14 is defined as the first width.
The blood taking needle is connected with the main bag through a second communicating pipe, the first communicating pipe is provided with a second one-way valve, the main bag is internally provided with an activity partition structure, the activity partition structure can partition the main bag, the main bag is divided into a left part and a right part, the upper side of the main bag is provided with at least one scale pipe, and the one-way communicating pipe is arranged on the lower side of the main bag.
The cord blood stem cell collector, the graduated flask is the cuboid cylinder shape, and its surface is provided with the scale, and its inboard sets up a plurality of check pressure valves along length direction, a plurality of check pressure valves switch on according to pressure from bottom to top, the graduated flask passes through a plurality of spaces are cut apart into to a plurality of check valves, and every space outside is provided with the sampling and detects the mouth.
The cord blood stem cell collector is characterized in that the movable partition structure is arranged in the middle of the main bag, the main bag can be arranged into a left part and a right part, the movable partition result comprises a first partition structure and a second partition structure, and when the partition is performed, the first partition structure is firstly subjected to partition operation, and then the second partition structure is subjected to partition operation; the first partition structure comprises an upper part and a lower part, the upper part and the lower part are respectively arranged on the inner surfaces of the front surface and the rear surface of the main bag, and the lower part comprises an arc with an opening, a first hook outside the arc with the opening and a first vertical baffle outside the first hook; the upper part comprises a round bar matched with the arc with the opening, a second hook coupled with the first hook and a second vertical baffle outside the second hook;
the second partition structure includes a pressure-bondable portion including a first width portion and a second width portion, the second width portion being larger than the first width portion, the second width portion being outside the first width portion, and a thermal adhesive seal member being provided between the first width portion and the second width portion.
When the umbilical cord blood stem cell collector is used for separating, firstly, the first separation structure is subjected to separation operation, and then, the second separation structure is subjected to separation operation, so that the method specifically comprises the following steps: applying a first external pressure to the first partition structure, pressing the first partition structure to divide the main bag into left and right two partitioned portions, applying a second external pressure to the first width portion of the second partition structure, applying a third external pressure to the second width portion, and applying a predetermined amount of heat to the thermal adhesive seal to completely partition the main bag.
In the cord blood stem cell collector and the sealing process of the second partition structure, the internal space of the main bag is compressed, and stem cells in the main bag are compressed into the at least one graduated tube.
The cord blood stem cell collector, the unidirectional flux tube outside is provided with the silica gel cap, the silica gel cap can puncture repeatedly.
The cord blood stem cell collector, still be provided with the rotating head on the first connecting pipe, the rotating head is in when the main bag carries out the centrifugation, can control the rotating head rotates.
The umbilical cord blood stem cell collector is characterized in that a temperature control box is further arranged on the outer side of the main bag, and a press machine, a rotating motor, a puncture needle, temperature control equipment, a controller, a sampling interface and an output interface are arranged in the temperature control box; the press is used for applying pressure to the movable partition structure so as to partition the main bag; the rotating motor is used for being connected with the rotating head and controlling the main bag to rotate and centrifuge; the puncture needle is used for puncture sampling of the one-way conduction tube and the graduated tube; the temperature control equipment is used for controlling the temperature of the temperature control box, and the temperature control equipment at least comprises two temperature control equipment, is arranged on the left side and the right side of the temperature control box respectively and is used for controlling the temperature of the left part and the right part of the main bag respectively; the sampling interface is used for connecting the sampling needle through the second communicating pipe; the output interface is used for being connected with the auxiliary bag through the first communication pipe; the controller is used for controlling the press machine, the rotating motor, the puncture needle and the temperature control equipment.
The umbilical cord blood stem cell collector, the temperature control box also comprises a first temperature sensor and a second temperature sensor, and the controller comprises a first comparison unit and a processing unit; the temperature control device comprises a first temperature sensor, a second temperature sensor, a first comparison unit, a processing unit, a standby temperature control unit, a standby reason investigation unit, a standby temperature control unit and a standby temperature control unit, wherein the first temperature sensor is used for acquiring a first temperature on the left side in a temperature control box, the second temperature sensor is used for acquiring a second temperature on the right side in the temperature control box, the first temperature and the second temperature are both sent to the first comparison unit in the controller, the first comparison unit is used for comparing the first temperature with the second temperature and then obtaining a first temperature difference value, if the first temperature difference value exceeds a first temperature threshold value, a first early warning temperature signal is sent to the processing unit, the processing unit examines the temperature and starts the standby temperature control unit, examines the abnormal reason, records the abnormal reason, and closes the standby temperature control unit after the abnormal reason is examined.
The cord blood stem cell collector collects cord blood samples through the sampling needle and conveys the cord blood samples to the main bag; the controller controls the rotating motor in the temperature control box to rotate, so that cord blood is fully mixed with anticoagulant in the main bag, after the preset time is waited, the rotating motor is controlled again to rotate and centrifuge, the first one-way valve is opened, and centrifuged plasma and red blood cells after stem cell separation are conveyed into the auxiliary bag through the first communicating pipe; the controller controls the movable partition structure to set the main bag into a left part and a right part, when the partition is executed, the press is controlled to apply pressure to the first partition structure to conduct partition operation, then the second partition structure is controlled to conduct partition operation, the second partition structure is controlled to conduct partition, meanwhile, the plurality of one-way pressure valves in the graduated tube are controlled to be opened, one part of stem cells are compressed into the graduated tube, and meanwhile, the temperature control box is divided into two independent temperature control spaces according to the fact that the movable partition structure is a dividing point;
during the storage period of the stem cells, the stem cells in at least one of the spaces in the graduated tube in the space on the left side of the temperature control box are collected at regular time from the sampling detection port through a puncture needle, and a first parameter is obtained; the controller judges the index state of the stem cells according to the first parameter, if the index state has deviation, the puncture needle punctures the one-way conduction pipe in the left space of the temperature control box to collect the stem cells, a second parameter is obtained, and the index state of the second parameter and the index state of the first parameter are compared;
if the second parameter is the same as the first parameter index, puncturing the unidirectional conduction pipe in the right space of the temperature control box to collect stem cells to obtain a third parameter, and comparing the third parameter with the first parameter and the second parameter; if the third parameter is different from the first parameter and the second parameter, the stem cells in the space on the left side of the temperature control box are independently extracted for manual detection to judge the reason of the deviation; if the third parameter is the same as the first parameter and the second parameter, extracting a sample to manually analyze the state of the stem cells;
if the second parameter is different from the first parameter index, whether the second parameter accords with the index state is judged, if so, stem cells in at least one of the spaces in the graduated tube in the space on the left side of the temperature control box are detected once again to obtain a first verification parameter, if the first verification parameter is the same as the second parameter index, the index is normal, if the first verification parameter is different from the second parameter index, the stem cells in the graduated tube are recorded to be damaged, and the next sampling is directly carried out from the one-way conduction tube in the space on the left side of the temperature control box.
Preferably, the graduated tube comprises one graduated tube, the graduated tube is arranged on the left side of the main bag, and the first parameter acquisition is carried out through the graduated tube;
preferably, the graduated tube can include two, set up respectively in main bag left side and main bag right side, gather two first parameters respectively through left side and right side graduated tube, through comparing two first parameters, carry out the index parameter comparison of main bag left and right sides to this index change of judging stem cell preservation. Preferably, when the first parameters of the left and right sides are different, the first parameters of the left and right sides can be cross-compared with the second parameters of the left and right sides.
The controller performs multiple sampling analysis through different comparison units and analysis units, can perform refined temperature control, and realizes long-time stable collection of high-precision umbilical cord blood stem cells from collection to storage detection.
Preferably, when the temperature is finely controlled, the quantity of the liquid nitrogen is adjusted in real time, full coverage control of the liquid nitrogen is realized, and the liquid nitrogen is kept in a temperature range and a liquid nitrogen state.
The beneficial effects of the invention include: 1. the cord blood stem cells are collected, separated and stored integrally, so that cord blood is conveniently and quickly collected; 2. the temperature can be regulated and controlled independently, and the periodic sampling can be carried out, so that the regular and accurate analysis and comparison in the preservation process are facilitated; 3. the processed blood plasma is quickly separated and integrally separated, so that the cost is saved, the separate separation processing in the backup process is reduced, and the separated stem cells are respectively stored through the movable partition structure after the integral separation processing, thereby being beneficial to reducing the stem cell pollution caused by improper storage, improving the stability of the stored stem cells and reducing the risk; 4. one of the main improvement points of the invention is that different partition is carried out by the first partition part and the second partition part, different isolation modes such as physical and thermal compression are structurally partitioned, the sealing performance of the partition is improved, the second partition part is combined with the space in the compression main bag, stem cells are pressed into the graduated tube in a matching manner, double functions are realized by one part, and the practicability of the part is improved. 5. The invention has the advantages that the controller can be used for carrying out sampling comparison on a plurality of regular divided spaces, the graduated tube, the left main bag and the right main bag are respectively used for carrying out sampling comparison to judge the storage state of the stem cells, accurate storage results are obtained through analysis and judgment, the deviation of detection results caused by inaccurate sampling or inaccurate samples in the graduated tube is avoided, meanwhile, the pollution of certain part of stem cells caused by the deviation of the temperature control devices can be prevented through different temperature control devices in the left temperature control space and the right temperature control space, and the storage stability of the stem cells is improved.
Claims (7)
1. A cord blood stem cell collector comprises a blood taking needle, a main bag, an auxiliary bag, a first communicating pipe and a second communicating pipe, wherein the main bag is communicated with the auxiliary bag through the first communicating pipe; the scale tube is in a cuboid cylindrical shape, scales are arranged on the outer surface of the scale tube, a plurality of one-way pressure valves are arranged on the inner side of the scale tube along the length direction, the one-way pressure valves are communicated from bottom to top according to pressure, the scale tube is divided into a plurality of spaces through the one-way pressure valves, and a sampling detection port is arranged on the outer side of each space; the movable partition structure is arranged in the middle of the main bag, the main bag can be arranged into a left part and a right part, the movable partition structure comprises a first partition structure and a second partition structure, when the partition is performed, the first partition structure is firstly subjected to partition operation, and then the second partition structure is subjected to partition operation; the first partition structure comprises an upper part and a lower part, the upper part and the lower part are respectively arranged on the inner surfaces of the front surface and the rear surface of the main bag, and the lower part comprises an arc with an opening, a first hook outside the arc with the opening and a first vertical baffle outside the first hook; the upper part comprises a round bar matched with the arc with the opening, a second hook coupled with the first hook and a second vertical baffle outside the second hook;
the second partition structure includes a pressure-bondable portion including a first width portion and a second width portion, the second width portion being larger than the first width portion, the second width portion being outside the first width portion, a thermal adhesive seal being provided between the first width portion and the second width portion; when the partition is performed, the first partition structure is firstly subjected to partition operation, and then the second partition structure is subjected to partition operation, which specifically comprises the following steps: applying a first external pressure to a first partition structure, pressing the first partition structure so as to divide the main bag into a left partition portion and a right partition portion, applying a second external pressure to a first width portion of a second partition structure, applying a third external pressure to the second width portion, and applying a predetermined amount of heat to the thermal bonding seal member so as to completely partition the main bag; different partitions are carried out through the first partition part and the second partition part, the second partition part is combined with the space in the compression main bag, stem cells are pressed into the graduated tube in a matched mode, and double functions are achieved through one part.
2. The cord blood stem cell collector of claim 1, wherein the second partition structure compresses the interior space of the main bag during the sealing process to compress the stem cells in the main bag into the at least one graduated tube.
3. The cord blood stem cell collector of claim 1, wherein a silica gel cap is arranged outside the one-way conduction tube, and the silica gel cap can be punctured repeatedly.
4. The cord blood stem cell collector of claim 1, wherein said first communication tube further comprises a rotating head, said rotating head can control said rotating head to rotate when said main bag is centrifuged.
5. The cord blood stem cell collector of claim 4, wherein a temperature control box is further arranged outside the main bag, and a press, a rotating motor, a puncture needle, a temperature control device, a controller, a sampling interface and an output interface are arranged in the temperature control box; the press is used for applying pressure to the movable partition structure so as to partition the main bag; the rotating motor is used for being connected with the rotating head and controlling the main bag to rotate and centrifuge; the puncture needle is used for puncture sampling of the one-way conduction tube and the graduated tube; the temperature control equipment is used for controlling the temperature of the temperature control box, and the temperature control equipment at least comprises two temperature control equipment, is arranged on the left side and the right side of the temperature control box respectively and is used for controlling the temperature of the left part and the right part of the main bag respectively; the sampling interface is used for connecting a blood taking needle through the second communicating pipe; the output interface is used for being connected with the auxiliary bag through the first communication pipe; the controller is used for controlling the press machine, the rotating motor, the puncture needle and the temperature control equipment.
6. The cord blood stem cell collector of claim 5, wherein the temperature control box further comprises a first temperature sensor and a second temperature sensor, and the controller comprises a first comparison unit and a processing unit; the temperature control device comprises a first temperature sensor, a second temperature sensor, a first comparison unit, a processing unit, a standby temperature control unit, a standby reason investigation unit, a standby temperature control unit and a standby temperature control unit, wherein the first temperature sensor is used for acquiring a first temperature on the left side in a temperature control box, the second temperature sensor is used for acquiring a second temperature on the right side in the temperature control box, the first temperature and the second temperature are both sent to the first comparison unit in the controller, the first comparison unit is used for comparing the first temperature with the second temperature and then obtaining a first temperature difference value, if the first temperature difference value exceeds a first temperature threshold value, a first early warning temperature signal is sent to the processing unit, the processing unit examines the temperature and starts the standby temperature control unit, examines the abnormal reason, records the abnormal reason, and closes the standby temperature control unit after the abnormal reason is examined.
7. The cord blood stem cell collector of claim 6, wherein said collector collects a cord blood sample through said blood collection needle and transfers it into said main bag; the controller controls the rotating motor in the temperature control box to rotate, so that cord blood is fully mixed with anticoagulant in the main bag, after the preset time is waited, the rotating motor is controlled again to rotate and centrifuge, the first one-way valve is opened, and centrifuged plasma and red blood cells after stem cell separation are conveyed into the auxiliary bag through the first communicating pipe; the controller controls the movable partition structure to set the main bag into a left part and a right part, when the partition is executed, the press is controlled to apply pressure to the first partition structure to conduct partition operation, then the second partition structure is controlled to conduct partition operation, the second partition structure is controlled to conduct partition, meanwhile, the plurality of one-way pressure valves in the graduated tube are controlled to be opened, one part of stem cells are compressed into the graduated tube, and meanwhile, the temperature control box is divided into two independent temperature control spaces according to the fact that the movable partition structure is a dividing point;
during the storage period of the stem cells, the stem cells in at least one of the spaces in the graduated tube in the space on the left side of the temperature control box are collected at regular time from the sampling detection port through a puncture needle, and a first parameter is obtained; the controller judges the index state of the stem cells according to the first parameter, if the index state has deviation, the puncture needle punctures the one-way conduction pipe in the left space of the temperature control box to collect the stem cells, a second parameter is obtained, and the index state of the second parameter and the index state of the first parameter are compared;
if the second parameter is the same as the first parameter index, puncturing the one-way conduction pipe in the right space of the temperature control box to collect stem cells to obtain a third parameter, and comparing the third parameter with the first parameter and the second parameter; if the third parameter is different from the first parameter and the second parameter, the stem cells in the space on the left side of the temperature control box are independently extracted for manual detection to judge the reason of the deviation; if the third parameter is the same as the first parameter and the second parameter, extracting a sample to manually analyze the state of the stem cells;
if the second parameter is different from the first parameter index, whether the second parameter accords with the index state is judged, if so, stem cells in at least one of the spaces in the graduated tube in the space on the left side of the temperature control box are detected once again to obtain a first verification parameter, if the first verification parameter is the same as the second parameter index, the index is normal, if the first verification parameter is different from the second parameter index, the damage of the stem cells in the graduated tube is recorded, and the next sampling is directly carried out from the one-way conduction tube in the space on the left side of the temperature control box.
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