CN214344599U - Flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells - Google Patents
Flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells Download PDFInfo
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- CN214344599U CN214344599U CN202023018902.8U CN202023018902U CN214344599U CN 214344599 U CN214344599 U CN 214344599U CN 202023018902 U CN202023018902 U CN 202023018902U CN 214344599 U CN214344599 U CN 214344599U
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Abstract
The utility model discloses a flat-pushing formula blood divides thick liquid to press from both sides for separation of umbilical cord blood hemopoietic stem cell, divide thick liquid to press from both sides one end and be equipped with fixed stripper plate, the opposite side is equipped with one and can follows the activity stripper plate that the slide removed, and mobile stripper plate is parallel with fixed stripper plate to the realization produces even pressure to umbilical cord blood separation bag. In addition, the lower part of the fixed extrusion plate is provided with a through elongated slot, so that a connecting small tube at the lower end of the cord blood bag can penetrate through the elongated slot, the upper layer of plasma and the lower layer of red blood cells can be discharged while the cord blood is centrifuged, the aim of centrifuging once and removing the plasma and the red blood cells simultaneously is fulfilled, and the cell recovery rate and the cell survival rate are improved.
Description
Technical Field
The utility model relates to a cord blood hematopoietic stem cell, in particular to a flat-pushing type blood plasma separating clamp for separating cord blood hematopoietic stem cell.
Background
The method for separating the umbilical cord blood hematopoietic stem cells directly influences the recovery rate and the survival rate of the hematopoietic stem cells. In the process of separating the umbilical cord blood, the survival rate of hematopoietic stem cells can be more effectively improved by reducing the centrifugation times; reduce the residue of red blood cells and partial plasma, and improve the recovery rate of hematopoietic stem cells, thereby obtaining more hematopoietic stem cells.
Currently, the cord blood separation technology is commonly used in a two-step centrifugation method: the method comprises the steps of firstly carrying out first centrifugation through density gradient centrifugation, harvesting the upper plasma layer and the middle leukocyte layer rich in nucleated cells, and removing the lower erythrocyte. Then, the second centrifugation is carried out to remove the redundant blood plasma on the upper layer, and finally, the tunica albuginea layer with the middle layer rich in nucleated cells is collected. The method uses two times of centrifugation, causes the loss of cells and the reduction of cell activity, and has certain influence on the quality of hematopoietic stem cells.
In addition, the separation of cord blood hematopoietic stem cells is usually carried out by a blood plasma separating clip with a V-shaped opening, namely, a fixed extrusion plate and a movable extrusion plate of the blood plasma separating clip are V-shaped. When the cord blood bag filled with the layering is placed into the V-shaped plasma separating clamp, because the extrusion force of the V-shaped clamp on the upper layer and the lower layer of the cord blood bag is uneven, the upper plasma in the cord blood bag is not completely removed and more residues are remained; (2) after the upper plasma in the cord blood bag is removed, the extrusion force at the bottom of the V-shaped clamp is large, so that the quantity of the lower red blood cells mixed into the tunica albuginea layer (namely the middle layer containing hematopoietic stem cells) is large, and the freezing storage and clinical application effects are influenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a flat-pushing formula blood plasma separating clamp for separation of umbilical cord blood hematopoietic stem cells is provided, has not only reduced the number of times of separation, improves hematopoietic stem cell activity, has improved the nucleated cell rate of recovery moreover, has reduced remaining of plasma and erythrocyte.
In order to solve the technical problem, the utility model discloses a technical scheme is: a flat-push type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells comprises a base, a fixed extrusion plate, a movable extrusion plate, a spring hinge, a sliding block, a sliding rail and a handle, wherein one end of the base is fixedly connected with the vertical fixed extrusion plate, one side edge of the lower part of the fixed extrusion plate is provided with a horizontally through elongated slot extending to the middle part of the fixed extrusion plate, the through elongated slot can enable a connecting tubule at the lower end of an umbilical cord blood bag to pass through, the base is provided with a groove perpendicular to the fixed extrusion plate, the sliding rail is installed in the groove, the sliding rail is sleeved with the sliding block capable of moving along the sliding rail, the sliding block is fixedly connected with the movable extrusion plate capable of contacting and being parallel with the fixed extrusion plate, the middle parts of two side edges of the movable extrusion plate are respectively provided with a lug seat with holes, the base below the fixed extrusion plate is provided with the spring hinge, end rods at two sides of the spring hinge are connected with two connecting rods forming acute angles with the two connecting rods and inserted into the rotating shafts at the lug seat holes at two sides of the back of the movable extrusion plate to enable the movable extrusion plate to pass through the rotating shafts Move the stripper plate and hug closely on fixed stripper plate surface, be connected with the control rod between two connecting rod spring end bar hinges department, be provided with the handle on the control rod, be provided with the couple at the base other end relative with fixed stripper plate, through pressing down the handle, make the slider of activity stripper plate below keep away from fixed stripper plate along the slide rail, the couple is impressed to the handle, activity stripper plate and fixed stripper plate separate, when loosening the handle, under the spring action of folding paper, the activity stripper plate moves to fixed stripper plate and pastes tightly with fixed stripper plate.
The top end of the through long groove is a round hole communicated with the through long groove.
The fixed extrusion plate is fixedly connected to the upper surface of the sliding block through a T-shaped support at the lower part of the back.
The base, the movable extrusion plate and the fixed extrusion plate are all rectangular, and the movable extrusion plate and the fixed extrusion plate are the same in size and shape.
The utility model has the advantages that: the movable extrusion plate is parallel to the fixed extrusion plate, so that the umbilical cord blood separation bag can generate uniform pressure. In addition, the lower part of the fixed extrusion plate is provided with a through elongated slot, so that a connecting small tube at the lower end of the cord blood bag can penetrate through the elongated slot, the upper layer of plasma and the lower layer of red blood cells can be discharged while the cord blood is centrifuged, the aim of centrifuging once and removing the plasma and the red blood cells simultaneously is fulfilled, and the cell recovery rate and the cell survival rate are improved.
Drawings
FIG. 1 is a schematic view of the closure of the horizontal push type blood plasma separating clamp for cord blood hematopoietic stem cell separation of the present invention.
Fig. 2 is a schematic diagram of the open horizontal push type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells according to the present invention.
Detailed Description
The technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiment of the present invention; obviously, the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention based on the embodiments of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "provided", "sleeved/connected", "connected", and the like are to be understood in a broad sense, such as "connected", which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in figures 1 and 2, the flat push type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells of the utility model comprises a base 8, a fixed extrusion plate 1, a movable extrusion plate 2, a spring hinge 12, a slide block 10, a slide rail 11 and a handle 7, wherein one end of the base 8 is fixedly connected with the vertical fixed extrusion plate 1, one side edge of the lower part of the fixed extrusion plate 1 is provided with a through elongated slot 13 extending to the horizontal direction of the middle part of the fixed extrusion plate 1, the through elongated slot 13 can enable a connecting tubule at the lower end of an umbilical cord blood bag to pass through, the base 8 is provided with a groove 15 vertical to the fixed extrusion plate 1, the slide rail 11 is arranged in the groove 15, the slide rail 11 is sleeved with the slide block 10 capable of moving along the slide rail, the slide block 10 is fixedly connected with the movable extrusion plate 2 capable of contacting and parallel with the fixed extrusion plate 1, the middle parts of two side edges of the movable extrusion plate 2 are respectively provided with a perforated ear seat 3, the spring hinge 12 is arranged on the base below the fixed extrusion plate 1, the two side end rods of the spring hinge 12 are connected with the rotating shafts 4 inserted into the two side ear seats 3 on the back of the movable extrusion plate 2 through the two connecting rods 5 forming acute angles with the two side end rods to tightly attach the movable extrusion plate 2 to the surface of the fixed extrusion plate 1, the control rod 6 is connected between the two connecting rods 5 and the hinge joint of the spring hinge end rods, the control rod 6 is provided with a handle 7, the other end of the base 8 opposite to the fixed extrusion plate 1 is provided with a hook 9, the slide block 10 below the movable extrusion plate 2 is far away from the fixed extrusion plate 1 along the slide rail 11 by pressing the handle 7 downwards, the handle 7 is pressed into the hook 9, the movable extrusion plate 2 is separated from the fixed extrusion plate 1, and when the handle 7 is loosened, the movable extrusion plate 2 moves towards the fixed extrusion plate 1 and is tightly attached to the fixed extrusion plate 1 under the elastic force action of the spring hinge 12.
The top end of the through long groove 13 is provided with a round hole 14 communicated with the through long groove 13.
The fixed extrusion plate 1 is fixedly connected to the upper surface of the sliding block 10 through a T-shaped support 16 at the lower part of the back.
In this embodiment, the base 8, the movable compression plate 2 and the fixed compression plate 1 are all rectangular, and the size and shape of the movable compression plate 2 and the fixed compression plate 1 are the same.
The cord blood was centrifuged by density gradient centrifugation, and when the blood plasma separator was used, the centrifugation parameters were 747g in centrifugal force, 15 minutes in time, and 10 ℃. After density gradient centrifugation, the umbilical cord blood in the blood collection bag is divided into three layers, the upper layer is plasma, the middle layer is a leucocyte layer rich in hematopoietic stem cells, and the lower layer is red blood cells.
The centrifuged umbilical cord blood is vertically placed in the middle of the opened blood plasma separating clamp, the connecting tubule at the lower end of the umbilical cord blood bag penetrates through the blood plasma separating clamp from the side surface to fix the through long groove of the extrusion plate, the umbilical cord blood bag is clamped in the round hole 14, the handle 7 is released, the movable extrusion plate 2 moves to the fixed extrusion plate 1 and is extruded, the plasma is discharged from the upper part of the umbilical cord blood bag, and the red blood cells are discharged from the connecting tubule at the lower end of the umbilical cord blood bag.
Use the utility model discloses cord blood plasma separator data comparison as follows:
with former blood plasma separation clamp with the utility model discloses an umbilical cord blood plasma separation clamp respectively separates 3 parts umbilical cord blood specimens, records umbilical cord blood volume around the separation, takes a sample respectively around the separation, carries out cell count, flow cytometer CD34+ cell detection, stem/progenitor cell culture, then carries out the analysis to the acquisition data.
First, comparison of recovery of nucleated cells (WBC) and Red Blood Cells (RBC)
Primary plasma clamp-WBC and RBC data records
The utility model discloses umbilical cord blood plasma separating clamp-WBC and RBC data record
Carry out cell count to the umbilical cord blood sample, can know by above data result, use the utility model discloses a sample that umbilical cord blood plasma separating clamp separated, under the condition that Red Blood Cell (RBC) clearance does not have obvious decline, nucleated cell (WBC) rate of recovery average can reach 92.1%, is obviously higher than the nucleated cell (WBC) rate of recovery 79.9% of former blood plasma separating clamp separation sample. The utility model discloses an improvement nucleated cell (WBC) rate of recovery that the umbilical cord blood plasma separator can be fine has better umbilical cord blood nucleated cell separation effect.
Two, CD34+Percentage comparison of cells
Primary blood plasma separating clip-CD 34+Cell data recording
The utility model discloses blood plasma separating clip-CD 34+Cell data recording
Performing flow cytometry on the umbilical cord blood sample, therebyAs a result, the cord blood treated by the cord blood plasma separator of the present invention has a high recovery rate of nucleated cells, so that CD34 is observed+The cell recovery rate is correspondingly improved, which shows that the cord blood plasma separating clamp of the utility model can well ensure the storage quality of the cord blood sample and better accord with the industry standard of stem cell transplantation.
Three, stem/progenitor cell culture results
To using the utility model discloses an umbilical cord blood sample after the umbilical cord blood plasma separation clamp is handled carries out stem/ancestor cell culture, and can know by above data result, uses the utility model discloses an umbilical cord blood plasma separation clamp carries out the umbilical cord blood after handling, and the equal normal growth of stem progenitor cell colony proves the utility model discloses an umbilical cord blood plasma separation clamp does not have the damage to stem progenitor cell, can fine assurance stem/ancestor cell's biological activity, guarantees the quality that umbilical cord blood stored.
In conclusion, the result analysis of the data shows that the umbilical cord blood plasma clamp of the utility model can obviously improve the recovery rate of nucleated cells (WBC) while ensuring the removal rate of Red Blood Cells (RBC); on the premise of ensuring the activity of the stem/progenitor cells, the recovery rate of CD34+ is also well improved. Prove the utility model discloses a production technology requirement when umbilical cord blood plasma separation presss from both sides can be better satisfies the separation of umbilical cord blood has created the advantage for the storage quality and the follow-up blood transplantation of umbilical cord blood sample.
In summary, the present invention is not limited to the above embodiments, and other embodiments can be easily proposed by those skilled in the art within the technical teaching of the present invention, but all such embodiments are included in the scope of the present invention.
Claims (4)
1. A flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells is characterized by comprising a base (8), a fixed extrusion plate (1), a movable extrusion plate (2), a spring hinge (12), a sliding block (10), a sliding rail (11) and a handle (7), wherein one end of the base (8) is fixedly connected with the vertical fixed extrusion plate (1), one side edge of the lower part of the fixed extrusion plate (1) is provided with a through long groove (13) which extends to the middle part of the fixed extrusion plate (1) in the horizontal direction, a connecting small tube at the lower end of an umbilical cord blood bag can pass through the through long groove (13), the base (8) is provided with a groove (15) which is vertical to the fixed extrusion plate (1), the sliding rail (11) is arranged in the groove (15), the sliding rail (11) is sleeved with a sliding block (10) which can move along the sliding rail, the sliding block (10) is fixedly connected with the movable extrusion plate (2) which can be contacted and parallel with the fixed extrusion plate (1), the middle parts of two side edges of the movable extrusion plate (2) are respectively provided with a lug seat (3) with a hole, a base below the fixed extrusion plate (1) is provided with a spring hinge (12), two side end rods of the spring hinge (12) are connected with two connecting rods (5) forming acute angles with the two side end rods and inserted into rotating shafts (4) in the holes of the lug seats (3) at two sides of the back of the movable extrusion plate (2) to tightly attach the movable extrusion plate (2) to the surface of the fixed extrusion plate (1), an operating lever (6) is connected between the two connecting rods (5) and the hinge joint of the spring hinge end rods, the operating lever (6) is provided with a handle (7), the other end of the base (8) opposite to the fixed extrusion plate (1) is provided with a hook (9), and a sliding block (10) below the movable extrusion plate (2) is far away from the fixed extrusion plate (1) along a sliding rail (11) by pressing the handle (7) downwards, the handle (7) is pressed into the hook (9), the movable extrusion plate (2) is separated from the fixed extrusion plate (1), and when the handle (7) is released, the movable extrusion plate (2) moves to the fixed extrusion plate (1) and is tightly attached to the fixed extrusion plate (1) under the action of the elastic force of the spring hinge (12).
2. The flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells according to claim 1, wherein the top end of the through long groove (13) is a round hole (14) communicated with the through long groove (13).
3. The flat-pushing type blood plasma separating clamp for cord blood hematopoietic stem cell separation according to claim 1, wherein the fixed pressing plate (1) is fixedly connected to the upper surface of the sliding block (10) through a T-shaped support (16) at the lower part of the back.
4. The flat-pushing type blood plasma separating clamp for cord blood hematopoietic stem cell separation according to any one of claims 1 to 3, wherein the base (8), the movable pressing plate (2) and the fixed pressing plate (1) are all rectangular, and the size and shape of the movable pressing plate (2) and the fixed pressing plate (1) are the same.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023018902.8U CN214344599U (en) | 2020-12-15 | 2020-12-15 | Flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202023018902.8U CN214344599U (en) | 2020-12-15 | 2020-12-15 | Flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells |
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| CN214344599U true CN214344599U (en) | 2021-10-08 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112675578A (en) * | 2020-12-15 | 2021-04-20 | 协和干细胞基因工程有限公司 | Flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells |
| CN114410424A (en) * | 2021-12-27 | 2022-04-29 | 四川新生命干细胞科技股份有限公司 | Device and method for separating nucleated cells of umbilical cord blood |
| CN114907943A (en) * | 2022-05-30 | 2022-08-16 | 五心生物科技(福建)有限公司 | Pulp separation device for umbilical cord blood hematopoietic stem cell inversion separation process |
-
2020
- 2020-12-15 CN CN202023018902.8U patent/CN214344599U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112675578A (en) * | 2020-12-15 | 2021-04-20 | 协和干细胞基因工程有限公司 | Flat-pushing type blood plasma separating clamp for separating umbilical cord blood hematopoietic stem cells |
| CN114410424A (en) * | 2021-12-27 | 2022-04-29 | 四川新生命干细胞科技股份有限公司 | Device and method for separating nucleated cells of umbilical cord blood |
| CN114907943A (en) * | 2022-05-30 | 2022-08-16 | 五心生物科技(福建)有限公司 | Pulp separation device for umbilical cord blood hematopoietic stem cell inversion separation process |
| CN114907943B (en) * | 2022-05-30 | 2023-09-26 | 五心生物科技(福建)有限公司 | Slurry separating device for umbilical cord blood hematopoietic stem cell inversion separation process |
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