CN205549007U - Be fit for biological sample storage bag that cell feedback was used - Google Patents
Be fit for biological sample storage bag that cell feedback was used Download PDFInfo
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- CN205549007U CN205549007U CN201620147007.0U CN201620147007U CN205549007U CN 205549007 U CN205549007 U CN 205549007U CN 201620147007 U CN201620147007 U CN 201620147007U CN 205549007 U CN205549007 U CN 205549007U
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Abstract
The utility model belongs to the field of medical equipment, a be fit for biological sample storage bag that cell feedback was used is related to, include: the first storage bag body and the second storage bag body arranged side by side, set up connecting line between the bag body between the first storage bag body and the second storage bag body for the biological sample can be followed a bag body and flowed in another bag of body, the first storage bag body is gone up along setting up an input port and being connected with the one end of connecting pipe through this input port, and the biological sample gets into the first storage bag body via the connecting pipe and through the input port, the first storage bag body is gone up along setting up first sample connection, and the second storage bag body is gone up along setting up the second sample connection, and first sample connection and second sample connection are used for taking out the storage at the internal biological sample of bag. This storage bag can conveniently be used for to the biological sample for example cord blood, haematopoietic stem cells, plasma etc. Are dealt with, the storage, freezing, come back to life or consciousness and the direct especially haematopoietic stem cells of patient's feedback biological sample that gives.
Description
Technical field
This utility model belongs to a kind of medical apparatus and instruments, specifically, this utility model relate to a kind of for biological sample such as Cord blood, make
Hemocytoblast, blood plasma particularly hematopoietic stem cell etc. are disposed, storage, freezing, recover and be directly used in the biological sample that treatment patient feeds back
Product storage bag.
Background technology
Along with the development of biology techniques, cell therapy is just fast-developing in worldwide and is day by day accepted by people.Cell therapy
It is acquisition and the storage of living cells at all.Additionally, the In vitro culture of cell has been widely applied to various biological study field.At body
In outer cell training, in order to preserve cell, and making it have biological activity for a long time, the frozen and recovery of cell becomes vital ring
Joint.And for the preparation of cell and storage, the especially low temperature storage of the biological sample such as Cord blood, stem cell, the condition of recovery and prevent behaviour
Make the pollution of process, be the important topic of current cell therapy clinical practice.Many countries and regions establish various cell bank, and optimization is thin
Born of the same parents' cryopreservation resuscitation program has become as, with preventing from polluting, the premise that cell produces and ensures cell quality.
Stem cell is the pluripotent cell that a class has the of self-replication capacity, and under certain condition, it can be divided into several functions cell.With
Deepening continuously of stem-cell research, the scope of its clinical practice the most constantly expands, and hematopoietic stem cell is to apply stem cell the earliest clinically,
Being the exclusive source of internal various hemocyte, it is primarily present in bone marrow, peripheral blood, Cord blood.The transplanting of hematopoietic stem cell is treatment blood
Liquid systemic disease, disease of immune system, ancestor genetic diseases and the multiple and effective ways of transitivity malignancy disease.
Along with deepening continuously of stem-cell research and stem cell clinical practice, hematopoietic stem cell transplantation has become radical cure malignant hematologic disease (white blood
Disease, malignant lymphoma, multiple myeloma), aplastic anemia, the effective means of some hereditary etc..Stem cell moves in recent years
Plant and be the most progressively applied to middle and advanced stage malignant solid tumor (small cell lung cancer, breast carcinoma, ovarian cancer, carcinoma of testis, neuroblastoma etc.), heart
The treatment of disease, nervous system injury, histoorgan reparation, diabetes, angiopathy etc..In addition stem cell also strengthen body immunity,
Repair pathological changes or the tissue of aging, increase man's lifespan, change the potential such as human survival state, there is immeasurable medical value.
But, the extensive clinical practice of stem cell must have the healthy source of human stem cell of abundance, and therefore, umbilical cord blood hematopoietic stem cell stores oneself to be become
Meet patient's needs, healthy stem cell can be provided whenever and wherever possible for the effective means of Clinical practice.Umbilical cord is set up in the approval of calendar year 2001 health ministry
Blood hemopoietic stem cell bank.And umbilical cord blood hematopoietic cell storage and application it is critical only that cell can be by the frozen of safety and recovery.
Classical hematopoietic stem cell transplantation process is, gathers the biological sample comprising hematopoietic cell > separate hemopoietic and be enriched with hematopoietic cell
Cell cryopreservation > cell recovery, if desired amplification > hematopoietic stem cell feeds back to patient.In the process, frozen to finally feeding back and can make
Cell is saved in the most frozen bag of same container, to avoid polluting, it is provided that a kind of frozen bag that can meet this long process is valuable
's.
Additionally, biological sample, such as Cord blood and goods thereof such as umbilical cord blood hematopoietic stem cell, need to carry out frozen, frozen after treatment
Before or frozen after be likely to require this biological sample detected, or for other purposes, or in these biological sample amounts not
Need in the case of foot to be completely used for therapeutic purposes.Therefore biological sample storage bag is particularly available for the frozen use of biological sample and can be straight
Connect for treating the biological sample storage bag that patient feeds back, to meet frozen, cell feedback, sterility barrier, the multi-functional purpose such as reserved.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of biological sample storage bag and is particularly available for the storage of the frozen use of biological sample
Bag, and expect that this storage bag disclosure satisfy that frozen, cell feedbacks, sterility barrier, the multi-functional purpose such as reserve.The most unexpectedly
Finding that the design using present patent application can meet one or more demands above-mentioned completely, therefore present patent application designs and is accomplished.
This utility model be employed technical scheme comprise that according to the realization of following one or more embodiments.
A kind of biological sample storage bag, it is characterised in that comprising:
The the first storage bag bag being arranged side by side and the second storage bag bag；
Between described first storage bag bag and the second storage bag bag, connecting line between bag is set so that biological sample can be from a bag stream
Enter another bag；
Along arranging an input port and being connected with one end of connecting tube by this input port on described first storage bag bag, biological sample is via connection
Manage and enter described first storage bag bag by input port；
Along arranging the first sample tap on described first storage bag bag, along arranging the second sample tap on described second storage bag bag, described first
Sample tap and the second sample tap take out for will be stored in the biological sample in bag.
According to biological sample storage bag of the present utility model, it is characterised in that described biological sample is selected from Cord blood, hematopoietic stem cell, blood plasma.
According to biological sample storage bag of the present utility model, it is characterised in that this biological sample storage bag is totally in flat.
According to biological sample storage bag of the present utility model, it is characterised in that between described bag, connecting line is provided with two, one is arranged on
Top between the first storage bag bag and the second storage bag bag that are arranged side by side, another is arranged on the first storage bag bag being arranged side by side
And the bottom that second between storage bag bag.
According to biological sample storage bag of the present utility model, it is characterised in that between described upper and lower two bags, connecting line can be melted by heat-sealing machine
Disconnected.Thus, when needed, after connecting line between upper and lower two bags being fused with heat-sealing machine, the first storage bag bag and the second storage bag bag
The memory space of both bodies is spaced, thus the biological sample being stored in them can not mutually circulate, and the biological sample in two storage bags can
For different purposes, such as the biological sample in a bag is used for treating, detect or the purpose such as amplification, the biological sample in another bag is used for treating,
The purposes such as detection or amplification.
According to biological sample storage bag of the present utility model, it is characterised in that the first storage bag bag being arranged side by side and the second storage bag bag,
And connecting line area defined is cut open between upper and lower two bags, form otch.Institute's shape after connecting line fusing between two bags
The fuse area become is easy to separate the first storage bag bag and the second storage bag bag together with above-mentioned otch.
According to biological sample storage bag of the present utility model, it is characterised in that the other end of described connecting tube is connected with sampling probe.This sampling probe
For biological sample is imported in storage bag.
According to biological sample storage bag of the present utility model, it is characterised in that described connecting tube can be fused by heat-sealing machine.Thus, at needs
Time, with heat-sealing machine, any one or more positions of connecting tube are fused.Particularly close to the first storage bag bag time at the one of the connecting tube that fuses
During the position of body, connecting tube can be separated with the storage bag being filled with biological sample.When fuse connecting tube two at time particularly one at close
The position of the first storage bag bag, at another close to the position of sampling probe one end time, can be by connecting tube and the storage bag being filled with biological sample
Separate, and remain in biological sample in connecting tube and seal and preserve for relevant purpose, such as, be used for treating, detect, the purpose such as amplification.
According to biological sample storage bag of the present utility model, it is characterised in that the tip of described sampling probe is three cavernous structures.This three poroid
Structure is possible to prevent the biological sample when sampling to block syringe needle.
According to biological sample storage bag of the present utility model, it is characterised in that be also configured with the cap bag sealed by this sampling probe on described sampling probe.
This cap bag enables to sampling probe particularly its syringe needle position and is under aseptic condition.
According to biological sample storage bag of the present utility model, it is characterised in that described connecting tube is also configured with this connecting tube to be closed or opening
Clip.
According to biological sample storage bag of the present utility model, it is characterised in that described first sample tap and the second sample tap are double-layer sealing structures.
Thus, in aseptic sealing state between inside and outside two-layer, when biological sample is sampled in storage bag by needs, outer layer is cut off, sterile needle
It is inserted in storage bag to extract biological sample from internal layer.
According to biological sample storage bag of the present utility model, it is characterised in that the volume of described first storage bag bag is the second storage bag bag
3～10 times of volume.
According to biological sample storage bag of the present utility model, it is characterised in that the volume of described first storage bag bag is the second storage bag bag
3～8 times of volume.
According to biological sample storage bag of the present utility model, it is characterised in that the volume of described first storage bag bag is the second storage bag bag
3～6 times of volume.
According to biological sample storage bag of the present utility model, it is characterised in that the volume of described first storage bag bag is the second storage bag bag
3 times of volume, 4 times, 5 times or 6 times.
According to biological sample storage bag of the present utility model, it is characterised in that described first storage bag bag and the second storage bag bag are by second
Alkene-vinylacetate (EVA), ethylene-methyl acrylate or straight-chain Low Density Polyethylene are made；Preferably, described frozen bag is by ethylene
-vinylacetate (EVA) is made.
According to biological sample storage bag of the present utility model, it is characterised in that described connecting tube be by ethylene-vinyl acetate (EVA), ethylene-
Methacrylate or straight-chain Low Density Polyethylene are made；Preferably, described frozen bag is made up of ethylene-vinyl acetate (EVA).
According to biological sample storage bag of the present utility model, it is characterised in that described first storage bag bag and the thickness of the second storage bag bag
For 100-500 μm；Preferably, the thickness of described frozen bag is 150-350 μm.
Arbitrary technical characteristic other arbitrary enforcement equally applicable that any embodiment of this utility model either side or this either side is had
Scheme or any embodiment of other either side, as long as they will not be conflicting, certainly at where applicable each other, if necessary may be used
Make individual features suitably to modify.Below various aspects of the present utility model and feature are further described.
All documents recited in this utility model, their full content is incorporated herein by, and if expressed by these documents
When implication is inconsistent with this utility model, it is as the criterion with statement of the present utility model.Additionally, the various terms of this utility model use and phrase tool
General sense known to capable field technique personnel, nonetheless, this utility model remains desirable to make more detailed at this to these terms and phrase
Description and interpretation, the term mentioned and phrase, if any inconsistent with common art-recognized meanings, are as the criterion with the implication that this utility model is stated.
Below various aspects of the present utility model are further described.
Biological sample storage bag of the present utility model, it can be directly placed into deep-bed drying under the liquid nitrogen environment of subzero 196 degrees Celsius.
Biological sample storage bag of the present utility model, its have excellence biocompatibility, can as biological sample such as Cord blood etc. return
Transport bag, is directly loaded up cell preparation, feeds back for therapeutic purposes to patient.
Biological sample storage bag of the present utility model, it has the sealability of excellence, can lead to according to the demand of different sample storage-uses
Cross heat-sealing machine to carry out fusing, closing.
For example, it is desired to during storage or when the cell concentration of feedback is big, two storage bag bags can be used together；Need the cell concentration fed back
Hour, it is possible to use allow heat-sealing machine by after two storage bag bag fusing, separately use respectively；Heat-sealing machine can be used two storage bag bags
After body fusing, being used as feeding back bag by one of them storage bag, another storage bag uses as the bag that keeps sample, and wherein biological sample is the thinnest
Born of the same parents keep sample detection.
Accompanying drawing explanation
Fig. 1 is the structural representation of biological sample storage bag of the present utility model.In figure, the labelling of each typical component is as follows: the first storage bag
Connecting line 3 between bag the 1, second storage bag bag the 2, first sample tap the 11, second sample tap 21, bag, input port 4, connecting tube 5,
Otch 6, sampling probe 7, cap bag 71, clip 8.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, this utility model is described in further detail.They are only more of the present utility model the most real
Executing mode, protection domain of the present utility model is not limited merely to these embodiments, and all technical schemes belonged under this utility model thinking all belong to
In protection domain of the present utility model.It should be pointed out that, for those skilled in the art, former without departing from this utility model
Some improvements and modifications under reason premise, should be regarded as protection domain of the present utility model.
In this utility model, during as used the space concept such as forward and backward, upper and lower, left and right, with this utility model biological sample storage bag
Input port, sample tap upward as top, direction the most as shown in Figure 1 is front view.
This utility model Fig. 1 schematically depict the structure of various aspects of the present utility model and spatial relationship thereof and working method, below
It is described further in conjunction with accompanying drawing.Some combine Fig. 1 the structure can being explicitly described with word and spatial relationship thereof and working method not
Illustrate in the drawings, but this has no effect on this utility model and clearly and completely describes various technical scheme of the present utility model.
Fig. 1 shows the structure of this utility model biological sample storage bag and spatial relationship thereof and working method.
Specifically, biological sample storage bag of the present utility model, including: the first storage bag bag 1 and the second storage bag bag being arranged side by side
Body 2；Between described first storage bag bag 1 and the second storage bag bag 2, connecting line 3 between bag are set so that biological sample can be from one
Bag flows into another bag；Along arranging an input port 4 one end by this input port 4 with connecting tube 5 on described first storage bag bag 1
Connecting, biological sample enters described first storage bag bag 1 via connecting tube 5 and by input port 4；On described first storage bag bag 1
Along arranging the first sample tap 11, along arranging the second sample tap 21 on described second storage bag bag 2, described first sample tap 11 and second takes
Sample mouth 21 takes out for will be stored in the biological sample in bag.
An embodiment according to this utility model biological sample storage bag, described biological sample is selected from Cord blood, hematopoietic stem cell, blood plasma.
An embodiment according to this utility model biological sample storage bag, this biological sample storage bag is totally in flat.This biological sample
Total storage volume of storage bag can be 10～500ml, and such as total storage volume can be 10～250ml, and such as total storage volume can be
10～100ml, such as total storage volume can be 20～50ml.
An embodiment according to this utility model biological sample storage bag, between described bag, connecting line 3 is provided with two, a setting
Top between the first storage bag bag 1 and the second storage bag bag 2 being arranged side by side, another is arranged on the first storage being arranged side by side
Bottom between bag bag 1 and the second storage bag bag 2.
An embodiment according to this utility model biological sample storage bag, between described upper and lower two bags, connecting line 3 can be by heat-sealing machine
Fusing.Thus, when needed, after being fused by connecting line 3 between upper and lower two bags with heat-sealing machine, the first storage bag bag 1 and second is deposited
The memory space of both bag bags 2 is spaced, thus the biological sample being stored in them can not mutually circulate, the life in two storage bags
Thing sample is available for different purposes, such as the biological sample in a bag is used for treating, detect or the purpose such as amplification, the biological sample in another bag
For treating, detect or the purpose such as amplification.
An embodiment according to this utility model biological sample storage bag, the first storage bag bag 1 and the second storage bag bag being arranged side by side
Between body 2, and upper and lower two bags, connecting line 3 four area defined is cut open, and forms otch 6.Connecting line 3 between two bags
The fuse area formed after fusing is easy to separate the first storage bag bag 1 and the second storage bag bag 2 together with above-mentioned otch 6.
An embodiment according to this utility model biological sample storage bag, the other end of described connecting tube 5 is connected with sampling probe 7.This takes
Sample pin 7 is for importing biological sample in storage bag.
An embodiment according to this utility model biological sample storage bag, described connecting tube 5 can be fused by heat-sealing machine.Thus, needing
When wanting, with heat-sealing machine, any one or more positions of connecting tube 5 are fused.Particularly deposit close to first time at the one of the connecting tube 5 that fuses
During the position of bag bag 1, connecting tube 5 can be separated with the storage bag being filled with biological sample.Spy time at the two of the connecting tube 5 that fuses
Be not at one close to the position of the first storage bag bag 1, at another close to the position of sampling probe 7 one end time, can by connecting tube 5 with fill
The storage bag having biological sample separates, and the biological sample sealing remained in connecting tube 5 preserves for relevant purpose, such as, be used for controlling
Treat, detect, the purpose such as amplification.
An embodiment according to this utility model biological sample storage bag, the tip of described sampling probe 7 is three cavernous structures.This three holes
Shape structure is possible to prevent the biological sample when sampling to block syringe needle.
An embodiment according to this utility model biological sample storage bag, described sampling probe 7 is also configured with sealed by this sampling probe 7
Cap bag 71.This cap bag 71 enables to sampling probe 7 particularly its syringe needle position and is under aseptic condition.
An embodiment according to this utility model biological sample storage bag, described connecting tube 5 be also configured with this connecting tube 5 being closed or
The clip 8 opened.
An embodiment according to this utility model biological sample storage bag, described first sample tap 11 and the second sample tap 21 are double-deck close
Seal structure.Thus, in aseptic sealing state between inside and outside two-layer, when biological sample is sampled in storage bag by needs, outer layer is cut off,
Sterile needle is inserted in storage bag to extract biological sample from internal layer.
An embodiment according to this utility model biological sample storage bag, is provided with at the first storage bag bag 1 being arranged side by side and
One-way valve (not shown) it is provided with in connecting line 3, so that aqueous biological sample can only between the bag of the bottom between two storage bag bags 2
Can not reverse flow to the second storage bag bag 2 direction one-way flow by the first storage bag bag 1.Have been found that this being designed with beneficially exists
Uniformly mixing the biological sample in two storage bags if desired and avoid cell aggregation, this is the most beat all.
An embodiment according to this utility model biological sample storage bag, the volume of described first storage bag bag 1 is the second storage bag bag
3～10 times of body 2 volume.
An embodiment according to this utility model biological sample storage bag, the volume of described first storage bag bag 1 is the second storage bag bag
3～8 times of body 2 volume.
An embodiment according to this utility model biological sample storage bag, the volume of described first storage bag bag 1 is the second storage bag bag
3～6 times of body 2 volume.
An embodiment according to this utility model biological sample storage bag, the volume of described first storage bag bag 1 is the second storage bag bag
3 times of body 2 volume, 4 times, 5 times or 6 times.
An embodiment according to this utility model biological sample storage bag, described first storage bag bag 1 and the second storage bag bag 2 are
It is made up of ethylene-vinyl acetate (EVA), ethylene-methyl acrylate or straight-chain Low Density Polyethylene；Preferably, described frozen bag by
Ethylene-vinyl acetate (EVA) is made.
An embodiment according to this utility model biological sample storage bag, described connecting tube 5 is by ethylene-vinyl acetate (EVA), second
Alkene-methacrylate or straight-chain Low Density Polyethylene are made；Preferably, described frozen bag is made up of ethylene-vinyl acetate (EVA).
An embodiment according to this utility model biological sample storage bag, described first storage bag bag 1 and the second storage bag bag 2
Thickness is 100-500 μm；Preferably, the thickness of described frozen bag is 150-350 μm.
Industrial applicability
The biological sample storage bag that this utility model relates to, be particularly suitable for biological sample in the case of different application demand, include that ultralow temperature is store
Biological sample is stored in the case of the demand of Tibetan.
The above, preferred embodiment the most of the present utility model, it is impossible to be construed to limit scope of the present utility model with this, all in this practicality
The deformation of the equivalent made in the scope that novel claims are claimed and the embodiment of change are all being protected required by this utility model
In the range of protecting.
Claims (19)
1. a biological sample storage bag, it is characterised in that comprising:
The the first storage bag bag being arranged side by side and the second storage bag bag；
Between described first storage bag bag and the second storage bag bag, connecting line between bag is set so that biological sample can flow into another bag from a bag；
Along arranging an input port and being connected with one end of connecting tube by this input port on described first storage bag bag, biological sample enters described first storage bag bag via connecting tube and by input port；
Along arranging the first sample tap on described first storage bag bag, along arranging the second sample tap, described first sample tap and the second sample tap for the biological sample taking-up that will be stored in bag on described second storage bag bag.
Biological sample storage bag the most according to claim 1, it is characterised in that described biological sample is selected from Cord blood, hematopoietic stem cell, blood plasma.
Biological sample storage bag the most according to claim 1, it is characterised in that this biological sample storage bag is totally in flat.
Biological sample storage bag the most according to claim 1, it is characterized in that, between described bag, connecting line is provided with two, one top being arranged between the first storage bag bag and the second storage bag bag being arranged side by side, another is arranged on the bottom between the first storage bag bag and the second storage bag bag being arranged side by side.
Biological sample storage bag the most according to claim 4, it is characterised in that between described upper and lower two bags, connecting line can be fused by heat-sealing machine.
Biological sample storage bag the most according to claim 5, it is characterised in that between the first storage bag bag being arranged side by side and the second storage bag bag, and upper and lower two bags, connecting line area defined is cut open, forms otch.
Biological sample storage bag the most according to claim 1, it is characterised in that the other end of described connecting tube is connected with sampling probe.
Biological sample storage bag the most according to claim 1, it is characterised in that described connecting tube can be fused by heat-sealing machine.
Biological sample storage bag the most according to claim 1, it is characterised in that described connecting tube is also configured with the clip this connecting tube can closed or open.
Biological sample storage bag the most according to claim 7, it is characterised in that the tip of described sampling probe is three cavernous structures.
11. biological sample storage bags according to claim 7, it is characterised in that be also configured with the cap bag sealed by this sampling probe on described sampling probe.
12. biological sample storage bags according to claim 1, it is characterised in that described first sample tap and the second sample tap are double-layer sealing structures.
13. biological sample storage bags according to claim 1, it is characterised in that the volume of described first storage bag bag is 3 ~ 10 times of the second storage bag bag volume.
14. biological sample storage bags according to claim 1, it is characterised in that the volume of described first storage bag bag is 3 ~ 8 times of the second storage bag bag volume.
15. biological sample storage bags according to claim 1, it is characterised in that the volume of described first storage bag bag is 3 ~ 6 times of the second storage bag bag volume.
16. biological sample storage bags according to claim 1, it is characterised in that the volume of described first storage bag bag is 3 times, 4 times, 5 times or 6 times of the second storage bag bag volume.
17. biological sample storage bags according to claim 1, it is characterised in that described first storage bag bag and the second storage bag bag are made up of ethylene-vinyl acetate, ethylene-methyl acrylate or straight-chain Low Density Polyethylene.
18. biological sample storage bags according to claim 1, it is characterised in that described connecting tube is made up of ethylene-vinyl acetate, ethylene-methyl acrylate or straight-chain Low Density Polyethylene.
19. biological sample storage bags according to claim 1, it is characterised in that the thickness of described first storage bag bag and the second storage bag bag is 100-500 μm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620147007.0U CN205549007U (en) | 2016-02-26 | 2016-02-26 | Be fit for biological sample storage bag that cell feedback was used |
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| CN201620147007.0U CN205549007U (en) | 2016-02-26 | 2016-02-26 | Be fit for biological sample storage bag that cell feedback was used |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943470A (en) * | 2019-03-26 | 2019-06-28 | 深圳市第二人民医院 | A kind of cord blood stem cell collection device |
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2016
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943470A (en) * | 2019-03-26 | 2019-06-28 | 深圳市第二人民医院 | A kind of cord blood stem cell collection device |
| CN109943470B (en) * | 2019-03-26 | 2022-06-17 | 深圳市第二人民医院 | Cord blood stem cell collector |
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