CN117413823A - Stem cell collection module - Google Patents
Stem cell collection module Download PDFInfo
- Publication number
- CN117413823A CN117413823A CN202311366713.5A CN202311366713A CN117413823A CN 117413823 A CN117413823 A CN 117413823A CN 202311366713 A CN202311366713 A CN 202311366713A CN 117413823 A CN117413823 A CN 117413823A
- Authority
- CN
- China
- Prior art keywords
- stem cell
- parameters
- unit
- parameter
- module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 353
- 239000003761 preservation solution Substances 0.000 claims abstract description 62
- 238000012545 processing Methods 0.000 claims abstract description 55
- 239000011550 stock solution Substances 0.000 claims abstract description 55
- 238000007689 inspection Methods 0.000 claims abstract description 49
- 238000000034 method Methods 0.000 claims abstract description 48
- 230000008569 process Effects 0.000 claims abstract description 48
- 238000004321 preservation Methods 0.000 claims abstract description 40
- 238000007710 freezing Methods 0.000 claims description 100
- 230000008014 freezing Effects 0.000 claims description 100
- 238000003860 storage Methods 0.000 claims description 92
- 238000001816 cooling Methods 0.000 claims description 84
- 239000007788 liquid Substances 0.000 claims description 29
- 239000002577 cryoprotective agent Substances 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 18
- 238000007781 pre-processing Methods 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims 3
- 238000000605 extraction Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 24
- 239000002609 medium Substances 0.000 description 18
- 238000011282 treatment Methods 0.000 description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 12
- 238000005138 cryopreservation Methods 0.000 description 11
- 238000012544 monitoring process Methods 0.000 description 10
- 238000001514 detection method Methods 0.000 description 7
- 238000004891 communication Methods 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 239000007790 solid phase Substances 0.000 description 6
- 239000013078 crystal Substances 0.000 description 5
- 230000006378 damage Effects 0.000 description 5
- 230000003834 intracellular effect Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 238000000926 separation method Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000005779 cell damage Effects 0.000 description 3
- 208000037887 cell injury Diseases 0.000 description 3
- 210000004700 fetal blood Anatomy 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 238000005192 partition Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241001155433 Centrarchus macropterus Species 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 229940041984 dextran 1 Drugs 0.000 description 1
- 229940119744 dextran 40 Drugs 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000009256 replacement therapy Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002660 stem cell treatment Methods 0.000 description 1
- 238000013190 sterility testing Methods 0.000 description 1
- ODLHGICHYURWBS-LKONHMLTSA-N trappsol cyclo Chemical compound CC(O)COC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)COCC(O)C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1COCC(C)O ODLHGICHYURWBS-LKONHMLTSA-N 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P90/00—Enabling technologies with a potential contribution to greenhouse gas [GHG] emissions mitigation
- Y02P90/02—Total factory control, e.g. smart factories, flexible manufacturing systems [FMS] or integrated manufacturing systems [IMS]
Abstract
The invention relates to a stem cell acquisition module. The stem cell acquisition module comprises an acquisition unit for acquiring stem cell stock solution, a pretreatment unit for processing the stem cell stock solution into stem cell preservation solution and a quality inspection unit for acquiring stem cell state, wherein the quality inspection unit can acquire the stem cell state of the stem cell stock solution acquired by the acquisition unit before entering the pretreatment unit, so that references are provided for processing parameter setting and processing parameter adjustment of the pretreatment unit. The present application provides for the first time an advanced classification of stem cells in connection with preservation and extraction. The characteristics of stem cells in stem cell stock solution and stem cell preservation solution and the characteristics of media in which the stem cells are positioned can be accurately grasped based on the first characteristics and the second characteristics of the stem cell states, so that accurate information reference is provided for the pretreatment process.
Description
The invention is a divisional application with the application number of CN202310821434.7, the application date of 2023, month 07 and 06, the application type of the invention and the application name of a stem cell collecting system.
Technical Field
The invention relates to the technical field of biological medical treatment, in particular to the technical field of biological tissue treatment and preservation, and specifically relates to a stem cell acquisition module.
Background
Stem cells are a class of cells from the embryo, fetus or adult body that have the ability to self-renew and proliferate and differentiate without restriction under certain conditions, and are capable of producing daughter cells of the exact same phenotype and genotype as themselves, and also of producing specialized cells that make up tissues and organs of the body, while also differentiating into progenitor cells. The multi-directional differentiation potential and self-renewal are the basic characteristics of stem cells, can be used as seed cells for cell therapy and tissue organ replacement therapy, can be used for discussing the regulation mechanism of embryo development or as a carrier for disease gene therapy, and can also support the establishment of a drug screening platform, pharmacological research and new drug development, so that the stem cells have extremely wide application prospects in the fields of life-science cell repair, developmental biology, pharmacology and the like.
In the current collection scheme for stem cells, the scheme is mainly based on collection structure setting and collection process control to achieve improvement of collection efficiency and collection quality. For example, patent publication No. CN114600871a discloses a stem cell cryopreservation apparatus comprising: one or more storage devices for freezing and storing the stem cells, a storage container for storing the storage devices, and a first transport device for transporting the storage devices into and out of the storage container; the storage device is provided with: a receiving portion for receiving one or more of the stem cell freezing vials, and a freezing tank for receiving a cold-keeping medium for freezing the stem cell freezing vials. The patent with the bulletin number of CN109943470B discloses a cord blood stem cell collector, which comprises a blood taking needle, a main bag, an auxiliary bag, a first communication pipe and a second communication pipe, wherein the main bag and the auxiliary bag are communicated through the first communication pipe, a first one-way valve is further arranged on the first communication pipe, the blood taking needle is connected with the main bag through the second communication pipe, a second one-way valve is arranged on the first communication pipe, a movable partition structure is arranged in the main bag, the movable partition structure can partition the main bag, the main bag is divided into a left part and a right part, at least one scale pipe is arranged on the upper side of the main bag, and a one-way guide pipe is arranged on the lower side of the main bag. According to the scheme, the separation structure or the multiple groups of structures are arranged in the stem cell collecting process and the storage process to realize optimal collection and backup storage, but the stem cell state and the stem cell processing parameters are not associated in the collecting process and the storage process to set, and the optimal freezing scheme cannot be set according to the stem cell state and the stem cell processing process.
Based on the above, the existing stem cell collection and storage scheme is less related to setting the collection device and the storage device in association with stem cell states, namely, the collection device is used for realizing targeted freezing pretreatment and the storage device with corresponding environmental parameters is arranged according to the stem cell states and storage requirements, and especially in the collection and storage processes of stem cells of different types, the stem cell parameters, the stem cell medium environment and the stem cell transportation states in the stem cell states are directly associated with the pretreatment process and the freezing process of the stem cells, so that the relevant parameters of the stem cell pretreatment process and the freezing process are required to be targeted according to the stem cell states, the applicability of the collection and storage device to different stem cells is ensured, and the operation efficiency and the operation quality of the collection and storage of the stem cells are improved based on the collection and storage parameters in the different stem cell states are adjusted.
Furthermore, there are differences in one aspect due to understanding to those skilled in the art; on the other hand, as the inventors studied numerous documents and patents while the present invention was made, the text is not limited to details and contents of all that are listed, but it is by no means the present invention does not have these prior art features, the present invention has all the prior art features, and the applicant remains in the background art to which the rights of the related prior art are added.
Disclosure of Invention
In view of at least some of the shortcomings set forth in the prior art, the present application provides a stem cell collection system comprising: the collecting module is used for obtaining stem cell stock solution and processing the stem cell stock solution into stem cell preservation solution; the storage module is used for freezing and preserving the stem cell preservation solution; the freezing module is used for cooling the acquisition module and/or the storage module; the intelligent module is used for controlling the processing process of the stem cell stock solution and the freezing preservation process of the stem cell preservation solution; the intelligent module sets processing parameters for processing the stem cell stock solution into the stem cell preservation solution based on the stem cell state of the stem cell stock solution, and sets a program-controlled cooling scheme executed by the freezing module on the storage module based on at least the stem cell state of the stem cell preservation solution, wherein the stem cell state at least comprises a first characteristic determined by the characteristics of the stem cells and a second characteristic determined by the environment in which the stem cells are positioned.
In the present application, the stem cell stock solution is a stem cell and a medium solution thereof which are extracted from a tissue and are subjected to examination without being subjected to processing before freezing, and the stem cell preservation solution is a stem cell and a medium solution thereof which are subjected to processing and can be directly used for freezing preservation.
The system of the application is based on the problem that the freezing treatment process and the freezing preservation process of the stem cells are not set pertinently based on the state of the stem cells, the freezing pretreatment process is carried out by the system based on the acquisition module to realize the freezing pretreatment of the stem cells, the freezing preservation process is carried out by the storage module to realize the freezing reduction treatment, and especially for the freezing treatment preservation of different types of stem cells, the first characteristics determined by the characteristics of the stem cells and the second characteristics determined by the environment where the stem cells are located in the state of the stem cells are directly associated with the freezing treatment process and the freezing preservation process.
The inventor creatively proposes a characteristic classification mode of stem cells related to preservation and extraction by means of a scientific classification mode for the first time, specifically in the application, the stem cell state comprises a first characteristic and a second characteristic, the first characteristic is an endogenous characteristic determined by the characteristics of the stem cells, and at least comprises category parameters, structural parameters and activity parameters of the stem cells, for example, the category parameters can be stem cell types, the structural parameters can be stem cell sizes, stem cell shapes and the like, and the activity parameters can be stem cell survival rate, growth state and the like; the second characteristic is an exogenous characteristic determined by the environment in which the stem cells are located, and includes at least a medium parameter, a temperature parameter, and a flow parameter of the stem cells, for example, the medium parameter may be a concentration of the stem cells and other components, the temperature parameter may be a temperature of the stem cells and a temperature of the medium of the stem cells, and the flow parameter may be a flow rate of the stem cells and the medium. The characteristics of stem cells in stem cell stock solution and stem cell preservation solution and the characteristics of media in which the stem cells are positioned can be accurately grasped based on the first characteristics and the second characteristics of the stem cell states, so that accurate information reference is provided for the freezing treatment process and the freezing preservation process.
Therefore, in order to realize the refinement operation of the stem cell freezing treatment so as to improve the cryopreservation quality of the stem cells, in the application, the processing parameters of the freezing treatment process and the program-controlled cooling scheme of the cryopreservation process are selected or adjusted based on the stem cell state, the processing parameters refer to relevant parameters of stem cell separation and purification and cryoprotection liquid configuration in the process of processing the stem cell stock solution into the stem cell preservation liquid, so that the stem cell stock solution can be processed into the stem cell preservation liquid meeting the cryopreservation condition, the program-controlled cooling scheme refers to the cooling interval and the cooling rate of the stem cell preservation liquid from normal temperature to the cryopreservation temperature, so that the stem cell preservation liquid can be matched with the stem cell state of the stem cell preservation liquid based on the setting adjustment of the cooling interval and the cooling rate in the program-controlled scheme, and the refinement operation process of the stem cell treatment freezing can be realized based on the parameter adjustment of different stem cell states.
Preferably, in the case where the collection module is configured with a pretreatment unit for processing the stem cell stock solution into the stem cell preservation solution, the intelligent module sets processing parameters for processing the stem cell stock solution into the stem cell preservation solution based at least on a stem cell state of the stem cell stock solution, including: the intelligent module collects the stem cell state of the stem cell stock solution before entering the pretreatment unit and selects and/or adjusts the processing parameters of the pretreatment unit through the comparison of the stem cell state and a preset rule.
Specifically, the intelligent module collects the stem cell state of the stem cell stock solution before entering the pretreatment unit and selects and/or adjusts the processing parameters of the pretreatment unit by comparing the stem cell state with a preset rule, including: the intelligent module acquires a plurality of parameters in a first characteristic and a second characteristic of the stem cell state in the stem cell stock solution based on monitoring acquisition or an external information source, compares and judges the plurality of parameters in the first characteristic and the second characteristic with preset freezing conditions in preset rules to obtain a first quality inspection result, and selects and/or adjusts processing parameters of the pretreatment unit based on the first quality inspection result. The preset freezing condition is a set threshold or set range set for a plurality of other parameters in the stem cell state based on the category parameters in the stem cell state, namely, the preset rule determines the proper state and the parameter range of the stem cell for freezing preservation, and the proper state and the stem cell state determined by the preset rule are compared, so that a setting or adjusting basis can be provided for the processing parameters of the pretreatment unit, and the stem cell state is processed into a state suitable for freezing preservation.
Preferably, in the case that the storage module is configured with a plurality of storage units that can be independently cooled by the freezing module, the intelligent module sets a programmed cooling scheme executed by the freezing module for the storage module based at least on a stem cell state of the stem cell preservation solution, including: the intelligent module collects the stem cell state of the stem cell preservation solution processed by the pretreatment unit, and selects a storage unit for freeze-drying the cell preservation solution and adjusts a program-controlled cooling scheme of the storage unit through comparison of the stem cell state and a preset rule.
Specifically, the intelligent module gathers the stem cell state of stem cell preservation solution after preprocessing unit processing and through the comparison of stem cell state and preset rule come the storage unit that is used for freeze-drying cell preservation solution and adjust the program control cooling scheme of storage unit, include: the intelligent module acquires a plurality of parameters of a first characteristic and a second characteristic of the stem cell state in the stem cell preservation liquid based on monitoring acquisition or an external information source, compares the plurality of parameters of the first characteristic and the second characteristic with preset freezing conditions in preset rules to obtain a second quality inspection result, selects a storage unit based on the second quality inspection result, and adjusts a program-controlled cooling scheme of the storage unit. The storage unit carries out freezing treatment based on a program-controlled cooling mode, and specific parameters of program-controlled cooling can be set differently based on stem cell states, so that the storage unit can carry out targeted freezing treatment on different types of stem cells of different biological targets, and the applicability of the system to stem cell preservation and long-term preservation quality are effectively improved.
Preferably, in the case that the preset freezing condition in the preset rule includes a threshold condition and a judging condition, the intelligent module compares and judges a plurality of parameters in the first feature and the second feature with the preset freezing condition in the preset rule to obtain a first quality inspection result or a second quality inspection result, including: the intelligent module obtains a first quality inspection result or a second quality inspection result based on the comparison result of the parameter range and/or the parameter value of a plurality of parameters in the stem cell state and the set value and/or the set range of the threshold condition and the judgment result of the comparison result by the judgment logic set by the judgment condition.
Preferably, the intelligent module selects and/or adjusts the processing parameters of the preprocessing unit based on the first quality inspection result at least includes: the intelligent module selects or adjusts one or more of centrifugal rotation speed and centrifugal time in centrifugal operation parameters of the pretreatment unit and substance category and substance proportioning concentration in cryoprotectant liquid configuration parameters based on the first quality inspection result.
Preferably, the intelligent module selects the storage unit based on the second quality inspection result and adjusts the program-controlled cooling scheme of the storage unit, and at least comprises: the intelligent module selects or adjusts a temperature reduction interval from a first temperature to a second temperature and a temperature reduction rate in the temperature reduction interval based on a second quality inspection result, wherein the first temperature is a temperature at which the stem cell preservation solution enters the storage unit, and the second temperature is a freezing preservation temperature of the stem cell preservation solution.
In addition, the intelligent module selects the storage unit based on the second quality inspection result and adjusts the program-controlled cooling scheme of the storage unit at least comprises: the intelligent module adjusts a cooling interval and a cooling rate in the cooling interval and a heating rate in the heating interval in the program-controlled cooling scheme based on the second quality inspection result, wherein the heating interval is set in a mode of changing the conversion temperature of the solid-liquid containing the cryoprotectant liquid.
The common cooling process is gradual gradient cooling, but due to the limitation of the dosage of the freezing protection liquid, a small amount of ice crystals still cause cell damage in cells in the gradient cooling process, and the cooling process is not single gradient cooling through changing the cooling gradient, but proper temperature rise is adopted when the temperature is lowered to a certain temperature node, so that the damage degree of the cells in freezing can be greatly reduced, and the temperature node can be set according to the liquid-solid phase transformation temperature determined by the ratio of the stem cell freezing protection liquid. The formula of the stem cell cryoprotectant liquid is different, the corresponding liquid-solid phase transition temperature is different when the program is cooled, and when the liquid-solid phase transition temperature of the stem cell cryoprotectant liquid is located in a temperature rising interval, the more the latent heat of the stem cell cryoprotectant liquid is absorbed, the ice crystal generation can be reduced to reduce the cell damage, and the survival rate of the stem cell before and after cryopreservation can be obviously improved to improve the cryopreservation quality.
Preferably, in the case that the preprocessing unit of the collecting module needs to perform batch processing on the stem cell stock solution and distribute the stem cell preservation solution to the plurality of storage units, the collecting module transmits the stem cell preservation solution based on a transport path respectively connected with the plurality of storage units and executes a corresponding program-controlled cooling scheme after the storage units of the storage module reach the storage total amount.
Drawings
FIG. 1 is a schematic diagram of a system functional connection of an embodiment of the present application;
fig. 2 is a schematic diagram of a system functional flow according to an embodiment of the present application.
List of reference numerals
100: an acquisition module; 101: an acquisition unit; 102: a preprocessing unit; 103: a transport unit; 104: a quality inspection unit; 200: a storage module; 201: a storage unit; 300: a freezing module; 301: a first cooling unit; 302: a second cooling unit; 400: an intelligent module; 401: a monitoring unit; 402: a transport control unit; 403: and a freezing control unit.
Detailed Description
Any direction set forth in this application is provided for reader convenience only and is not a corresponding limitation of this application. In the description of the present invention, it should be understood that the terms "center," "longitudinal," "transverse," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," and the like indicate an orientation or a positional relationship based on that shown in the drawings, merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements in question must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the invention.
The present invention will be described in detail with reference to the accompanying drawings.
The application provides a stem cell collecting system, especially relates to a stem cell collection and storage system, especially relates to a stem cell intelligent collecting system, is called the system for short below, belongs to biomedical technical field, can be used to carry out targeted collection processing and cryopreservation to the stem cells of different grade and under the different state.
As shown in fig. 1, the system of the present application includes an acquisition module 100 for acquiring stem cell stock solution and processing the stem cell stock solution into stem cell preservation solution, a storage module 200 connected to the acquisition module 100 and used for cryopreserving the stem cell preservation solution, a freezing module 300 acting on the acquisition module 100 and the storage module 200 to realize a low temperature environment, and an intelligent module 400 for controlling the acquisition process and the storage process.
In the application, the stem cell stock solution is stem cells and a medium solution thereof which are extracted from tissues and are subjected to detection without pre-freezing processing treatment, the stem cell preservation solution is stem cells and the medium solution thereof which can be directly used for freezing preservation after processing treatment, and the stem cell stock solution is processed into the stem cell preservation solution which comprises the steps of detection, separation and purification, freezing protection solution addition, transportation control and the like.
Specifically, as shown in fig. 1 and 2, in order to effectively obtain a stem cell stock solution and process it into a stem cell preservation solution, the collection module 100 is configured with a collection unit 101 for obtaining the stem cell stock solution, a preprocessing unit 102 for processing the stem cell stock solution into the stem cell preservation solution, and a transport unit 103 for transporting the stem cell preservation solution to the storage module 200. The acquisition of the stem cell stock solution by the acquisition unit 101 can be implemented based on an acquisition device in the prior art, and the acquisition device can be a corresponding manual device or an automatic machine; or the collection unit 101 is directly connected to an existing stem cell collection device or storage container to obtain a stem cell stock solution for cryopreservation under control. For example, the stem cell stock solution source may include peripheral blood hematopoietic stem cells, bone marrow hematopoietic stem cells, cord blood hematopoietic stem cells, and the like. Furthermore, the collected stem cell stock solution needs to be subjected to admission inspection and quality inspection before being subjected to freezing pretreatment and freezing preservation (namely, preparation, production and preservation stages). Admission testing is the process of rapid detection of biosafety, pathogens, etc. on samples (e.g., fat, blood, umbilical cord, placenta, etc.) that may be exposed to collection after the sample is retrieved from the collection site, prior to entering the preparation, production, and storage phase. And (3) admitting the sample which is detected to be qualified into the preparation production preservation stage, carrying out subsequent preparation production, and timely destroying the unqualified sample according to a program after recording, so that the sample cannot enter the preparation production preservation stage. Quality inspection is a more comprehensive quality inspection performed to ensure the safety, effectiveness and quality controllability of stem cells after specific in vitro treatments. Quality test items for stem cells include, but are not limited to: cell identification, viability, growth activity, purity and homogeneity, sterility testing and mycoplasma detection, detection of intracellular exogenous pathogenic agents, endotoxin detection, abnormal immunological reactions, tumorigenicity, biological efficacy testing, detection of residual amounts of culture media and other additional components, and the like. The stem cell stock solution obtained based on the acquisition module 100 is stem cells passing admission inspection and quality inspection, so that the stem cell stock solution can be prepared, produced and stored by the acquisition module 100 and the freezing module 300. In addition, the stem cell stock solution cannot be directly frozen for preservation, and the stem cell stock solution needs to be treated and added with a corresponding freezing protection solution, because a large amount of other unneeded component substances exist in the stem cell stock solution, the main components inside and outside the cell are water, and if the stem cell stock solution is directly frozen without the protection solution, the water inside and outside the cell can be condensed into ice crystals, and endogenous mechanical damage of the cell is caused. Cell dehydration due to the condensation of intracellular moisture also causes changes in intracellular pH, protein denaturation, loss of intracellular organ function, and even nuclear DNA damage, which is highly likely to lead to cell death. Most of the cryoprotectant is a small molecular substance which is easy to dissolve, has cell permeability, can penetrate through cell membranes, enter cells, lower the freezing point, and can improve the permeability of the cell membranes to water, so that the water in the cells seeps out of the cells, and the formation of ice crystals in the cells is reduced.
Therefore, the stem cell stock solution obtained by the collection unit 101 needs to be processed into a stem cell preservation solution usable for direct cryopreservation by the pretreatment unit 102 and transferred to the storage module 200 by the transportation unit 103 for cryopreservation. In order to effectively control the pretreatment process of the stem cell stock solution and obtain the stem cell preservation solution meeting the preset freezing conditions, the acquisition module 100 is further configured with a quality inspection unit 104 for acquiring the stem cell state, and the quality inspection unit 104 can acquire the stem cell state of the stem cell stock solution before entering the pretreatment unit 102, so that references are provided for the processing parameter setting and the processing parameter adjustment of the pretreatment unit 102; the quality inspection unit 104 can also collect the stem cell state of the stem cell preservation solution, and the comparison result of the stem cell state of the stem cell preservation solution and the preset freezing condition can be used as the basis for feedback adjustment of the processing parameters of the preprocessing unit 102, so that the collection module 100 can realize the accuracy and effectiveness of the preprocessing process through the action of the quality inspection unit 104. The comparison result of the stem cell state of the stem cell preservation solution and the preset freezing condition can also be used for configuring a program-controlled cooling scheme matched with the stem cell state of the stem cell preservation solution in the corresponding storage units 201, so that the plurality of storage units 201 of the storage module 200 can respectively store different types of stem cells and can be set in a targeted manner according to the stem cell state of the stem cell preservation solution.
The application creatively provides an advanced classification mode of stem cells related to preservation and extraction for the first time, which comprises the following specific steps: the stem cell state includes a first feature and a second feature, the first feature is an endogenous feature determined by the characteristics of the stem cell, and at least includes a category parameter, a structural parameter and an activity parameter of the stem cell, for example, the category parameter may be a stem cell type, the structural parameter may be a stem cell size, a stem cell shape and the like, and the activity parameter may be a stem cell survival rate, a growth state and the like; the second characteristic is an exogenous characteristic determined by the environment in which the stem cells are located, and includes at least a medium parameter, a temperature parameter, and a flow parameter of the stem cells, for example, the medium parameter may be a concentration of the stem cells and other components, the temperature parameter may be a temperature of the stem cells and a temperature of the medium of the stem cells, and the flow parameter may be a flow rate of the stem cells and the medium. The characteristics of stem cells in stem cell stock solution and stem cell preservation solution and the characteristics of media in which the stem cells are positioned can be accurately grasped based on the first characteristics and the second characteristics of the stem cell states, so that accurate information reference is provided for a pretreatment process and a freezing process. The preset freezing condition is a set threshold or a set range set for a plurality of other parameters in the stem cell state based on the category parameters in the stem cell state, so that the stem cells meeting the set threshold or the set range can meet the parameter requirements of freezing preservation and achieve good freezing preservation effect. The processing parameters comprise centrifugal operation parameters and cryoprotectant solution configuration parameters, wherein the centrifugal operation parameters can be centrifugal rotation speed and centrifugal time for separating stem cells and medium solution in stem cell stock solution; the cryoprotectant solution configuration parameter may be a species type and a species proportioning concentration of the cryoprotectant solution.
Preferably, the storage module 200 is configured with a plurality of storage units 201 capable of independently performing cooling control, and the plurality of storage units 201 are respectively connected with the transportation unit 103 of the collection module 100, so that the plurality of storage units 201 can be used for storing different types of stem cells of different biological targets. The storage unit 201 performs freezing treatment based on a program-controlled cooling mode, and specific parameters of program-controlled cooling can be set differently based on stem cell states, so that the storage unit 201 can perform targeted freezing treatment on different types of stem cells of different biological targets, and the applicability of the system to stem cell preservation and long-term preservation quality are effectively improved.
Under normal conditions, stem cells can be stored for 5 hours at normal temperature, can be stored for 48 hours at about minus 8 ℃, can be stored for one month at minus 80 ℃ in a cryogenic refrigerator, and can be stored permanently in theory at minus 196 ℃ in liquid nitrogen. Therefore, the freezing module 300 is configured with a first cooling unit 301 for cooling the collection module 100 and a second cooling unit 302 for cooling the storage module 200, where the first cooling unit 301 is used to keep the collection module 100 at a low temperature above zero, so that adverse effects of normal temperature on the activity of stem cells in the collection module 100 can be avoided, and the quality of stem cells transferred from the collection module 100 to the storage module 200 is improved. The second cooling unit 302 is configured to perform programmed cooling on the plurality of storage units 201 of the storage module 200 and finally achieve a liquid nitrogen temperature, so that the stem cells contained in the storage units 201 can be stored for a long time or even permanently.
To achieve the above-mentioned efficient control of the pretreatment process and the freezing process, the intelligent module 400 of the system is configured with at least a monitoring unit 401 for acquiring the state of stem cells, a transport control unit 402 for controlling the transfer of stem cells from the collection module 100 to the storage module 200, and a freezing control unit 403 for controlling the freezing module 300 to perform the cooling process, the monitoring unit 401 is in data connection with the quality inspection unit 104 of the collection module 100, so that the monitoring unit 401 can share the data related to the state of stem cells acquired by the quality inspection unit 104.
Preferably, to effectively control the pretreatment process of the stem cell stock solution to obtain a stem cell preservation solution meeting a preset freezing condition, the intelligent module 400 collects the stem cell state of the stem cell stock solution before entering the pretreatment unit 102 based on the quality inspection unit 104 and selects and/or adjusts the processing parameters of the pretreatment unit 102 by comparing the stem cell state with a preset rule, including: and acquiring a plurality of parameters in the first characteristic and the second characteristic of the stem cell state in the stem cell stock solution based on monitoring acquisition or an external information source, comparing and judging the plurality of parameters in the first characteristic and the second characteristic with preset freezing conditions in preset rules to obtain a first quality inspection result, and selecting and/or adjusting processing parameters of the pretreatment unit 102 based on the first quality inspection result. Specifically, the monitoring and collecting of stem cells can be implemented by a sensor and an existing measuring device, and is mainly used for obtaining stem cell state related parameters which can be obtained by real-time measurement, such as a structural parameter in a first feature and a temperature parameter and a flow parameter in a second feature, and an external information source can be used for inputting stem cell state related parameters which are inconvenient to obtain by real-time measurement, such as a category parameter in the first feature, an activity parameter, a medium parameter in the second feature and the like.
For example, in an external source of information, the class parameters for obtaining stem cell status can be used to distinguish and identify various stem cells by virtue of their surface markers, which are specific protein receptors coated on the cell surface that selectively bind or adhere to other signaling molecules; the STEM cell distribution concentration may be obtained based on a STEM cell counter, such as a LUNA-STEM fluorescent cell counter; stem cell viability to obtain stem cell status can be obtained based on a flow cytometer, such as the sammer flier Attune NXT flow cytometer.
Preferably, the preset freezing conditions in the preset rules define threshold conditions and judgment conditions suitable for performing freezing processing, and the comparison result is judged based on the comparison result of the parameter value or parameter range in the stem cell state and the set value or set range of the threshold conditions and judgment logic set by the judgment conditions to obtain a first quality inspection result, wherein the first quality inspection result comprises a selection adjustment instruction for the processing parameters of the pretreatment unit 102, and the selection adjustment instruction is used for selecting or adjusting the processing parameter value or the processing parameter range of the pretreatment unit 102, such as the centrifugal rotation speed and the centrifugal time in the centrifugal operation parameters and the substance category and the substance proportioning concentration in the cryoprotectant liquid configuration parameters.
Specifically, the preset freezing conditions in the preset rule may be set based on the stem cell type, for example, the preset freezing conditions include: stem cell size setting range, normal shape and density of stem cells which are based on the types of the stem cells are about 80%, and activity is 90%; setting the stem cell concentration count to be 10 6 -10 7 The stem cell temperature and stem cell flow rate are set to a range. The intelligent module 400 obtains relevant parameters in the stem cell state based on monitoring collection or an external information source, and compares the relevant parameters with the preset freezing conditions to determine that a first quality inspection result is obtained, for example, when the stem cell concentration count is smaller than a value of a set range, the processing parameters of the pretreatment unit 102 need to be set or adjusted, that is, the centrifugal rotation speed and the centrifugal time in the centrifugal operation in the processing parameters are set or adjusted according to the deviation between the stem cell concentration count and the set range, the stem cell stock solution is separated and purified, and after the freezing protection solution is added, the stem cell concentration count can meet the set range of the preset freezing conditions. The preset freezing conditions of the preset rules are also set on the basis of the stem cell state with respect to the disposition scheme of the cryoprotectant, for example, the substance class of the cryoprotectant used by the stem cell is determined on the basis of the type of the stem cell, the proportioning concentration of the cryoprotectant is set on the basis of several parameters in the stem cell state, for example, the cryoprotectant of the first type is generally an osmotic type, such as glycerol or 10% dimethyl sulfoxide (DMSO) and 10% -90% fetal bovine serum, and for example, the cryoprotectant of the stem cell is composed of the following substances: the concentration ratio of each component is as follows: 8 to 10 percent of dimethyl sulfoxide (DMSO), 10 to 20 percent of human serum, 2 to 6 (w/v) percent of trehalose, 3 to 7 (w/v) percent of dextran and 0.85 to 0.9 percent of physiological saline, wherein the weight proportions of the components are as follows: dimethyl sulfoxide 1, human serum 2, trehalose 1, dextran 1 and physiological saline 5; for example, the second type of cryoprotectant may comprise 5% human serum albumin5 percent of dimethyl sulfoxide, 40 percent to 60 percent of compound electrolyte, 1 percent to 10 percent of sodium chloride solution with the mass concentration of 0.9 percent, 1 percent to 10 percent of glucose solution with the mass concentration of 10 percent, 1 percent to 10 percent of dextran 40 glucose solution and 0.5 percent to 5 percent of hydroxypropyl-beta-cyclodextrin.
Therefore, the first quality inspection result based on the quality inspection unit 104 can be used to determine the processing parameters of the pretreatment unit 102, that is, to explicitly select an appropriate processing scheme for different types of stem cells and different stem cell states, including separation and purification of stem cells and impurity removal based on centrifugal operation parameters, and adding and mixing of the cryoprotectant solution based on cryoprotectant configuration parameters, so that the pretreatment unit 102 can purposefully process the stem cell stock solution into the stem cell preservation solution meeting the freezing requirement.
Preferably, the quality inspection unit 104 can also collect the stem cell state of the stem cell preservation solution, and the comparison result of the stem cell state of the stem cell preservation solution and the preset freezing condition can be used as the basis for feedback adjustment of the processing parameters of the preprocessing unit 102, so that the collection module 100 can realize the accuracy and effectiveness of the preprocessing process through the action of the quality inspection unit 104. Generally, the pretreatment unit 102 may process a stem cell stock solution with a certain volume according to the device structure and pretreatment capacity of the pretreatment unit 102 at the same time or in batches, and then in the case of batch treatment, the comparison result of the stem cell state of the stem cell preservation solution obtained by pretreatment and the preset freezing condition may be used to perform feedback adjustment on the processing parameters of the pretreatment unit 102.
Preferably, to obtain the stem cell state of the stem cell preservation solution processed by the preprocessing unit 102, the intelligent module 400 collects the stem cell state of the stem cell preservation solution processed by the preprocessing unit 102 based on the quality inspection unit 104 and selects the storage unit 201 for freeze-drying the cell preservation solution and adjusts the programmed cooling scheme of the storage unit 201 by comparing the stem cell state with a preset rule, including: and acquiring a plurality of parameters of the first characteristic and the second characteristic of the stem cell state in the stem cell preservation solution based on monitoring acquisition or an external information source, comparing and judging the plurality of parameters of the first characteristic and the second characteristic with preset freezing conditions in preset rules to obtain a second quality inspection result, selecting the storage unit 201 based on the second quality inspection result, and adjusting a program-controlled cooling scheme of the storage unit 201. Specifically, the preset freezing conditions in the preset rules are used for adjusting a program-controlled cooling scheme by means of the comparison result of the stem cell state, the threshold condition and the judgment condition, and the program-controlled cooling scheme at least comprises a cooling interval and a cooling rate. The program-controlled cooling is a cooling mode of controlling the release of liquid nitrogen according to a computer set program to control the release of liquid nitrogen so as to achieve the aim of accurate cooling, and the program-controlled cooling can select the optimal cooling rate based on parameter setting and can be used for balancing 'intracellular ice damage' and 'solute damage' so as to improve the freezing quality.
Further, the threshold condition and the judgment condition of the preset freezing condition specify stem cell states suitable for different program-controlled cooling schemes, and the different program-controlled cooling schemes can be set and implemented based on the storage unit 201 of the independent cooling control, so that a second quality inspection result obtained by comparing a plurality of parameters of the first characteristic and the second characteristic of the stem cells with the preset freezing condition in the preset rule can be used as a setting basis of the program-controlled cooling scheme. Meanwhile, the transportation control unit 402 of the intelligent module 400 adjusts transportation parameters of the transportation unit 103 of the collection module 100, such as the flow rate of the stem cell preservation solution and the total amount of the stem cell preservation solution, based on the second quality inspection result and the set transportation parameters determined by the program-controlled cooling scheme of the storage unit 201, so that the transportation parameters of the transportation unit 103 are matched with the program-controlled cooling scheme of the storage unit 201 to ensure the freezing quality.
For example, medium parameters of the second characteristic in the stem cell state of the stem cell preservation solution, such as the substance category and the proportioning concentration of the cryoprotectant, are directly related to the program-controlled cooling scheme of the storage unit 201, and based on the freezing experiment and the empirical data, a plurality of groups are set for different cryoprotectant substance categories and proportioning concentrations in the stem cell preservation solution, and corresponding program-controlled cooling schemes are set for each group, so that the cooling scheme corresponding to the program-controlled cooling scheme matches the medium parameters of the stem cell preservation solution to ensure the program-controlled cooling freezing quality. The key point of the program-controlled cooling scheme is that the liquid-solid phase transformation process of the stem cell preservation liquid is characterized in that the liquid-solid phase transformation temperature of the stem cell preservation liquid is changed due to the difference of the proportions of the freezing protection liquid, and the program-controlled cooling scheme can be set in a targeted mode at least according to the proportions of the freezing protection liquid. For example, for a stem cell preservation fluid to which a first type of cryoprotectant is added, the programmed cooling regimen comprises: cooling to-7deg.C or-11deg.C at a speed of 1deg.C/min; reducing the temperature to the box body to 50 ℃ below zero at the speed of 50 ℃/min; raising the temperature to-25 ℃ at a speed of 15 ℃/min, and keeping the temperature at-25 ℃ for 3min; cooling to specimen-50deg.C or box-60deg.C at a speed of 1deg.C/min; cooling to-90deg.C or-120deg.C at 10deg.C/min, and storing at liquid nitrogen temperature for a long period, wherein the container is the shell of the storage unit 201 contacting with stem cell preservation solution. For example, the program-controlled cooling scheme of the stem cell preservation solution added with the second type of cryoprotectant comprises the following steps: waiting for 3-4min at 4deg.C; cooling to the sample temperature of-5 ℃ at a speed of 1 ℃/min; cooling to-54 ℃ at a speed of 21 ℃/min; cooling to-17 ℃ at a speed of 17 ℃/min; cooling to-40 ℃ at a speed of 2 ℃/min; cooling at a speed of 10deg.C/min to sample temperature of-80deg.C, cooling to liquid nitrogen temperature, and storing for a long time. Generally, the cooling process is gradual gradient cooling, but due to the limitation of the use amount of the cryoprotectant, a small amount of ice crystals still cause cell damage in cells in the gradient cooling process; the temperature reduction process is not single gradient temperature reduction by changing the temperature reduction gradient, but proper temperature rise is adopted when the temperature is reduced to a certain temperature node, so that the damage degree of cells in freezing storage can be greatly reduced, and the temperature node can be set according to the ratio of the freezing protection liquid and the liquid-solid phase conversion temperature of the stem cell preservation liquid.
Preferably, after determining the storage unit 201 and the programmed cooling scheme, the intelligent module 400 determines a set transport parameter suitable for the current programmed cooling scheme, and the transport control unit 402 of the intelligent module 400 controls the transport unit 103 of the collection module 100 based on the set transport parameter, so that the transport unit 103 can transfer the stem cell preservation solution processed by the pretreatment unit 102 to the corresponding storage unit 201 of the storage module 200 according to the set transport parameter. The transportation parameters may include a transportation path, a transportation flow rate and a total transportation amount, especially, in the case that the pretreatment unit 102 needs to perform batch processing on the stem cell stock solution and distribute the stem cell preservation solution to the storage units 201, the storage units 201 are respectively provided with the transportation paths connected with the transportation units 103, and then the transportation units 103 can respectively transmit the stem cell preservation solution corresponding to the total transportation amount at the transportation flow rates specified by the set transportation parameters based on different directions of the transportation paths, so that the total storage amount of the corresponding stem cell preservation solution in the storage units 201 can conform to the expected program-controlled cooling scheme set in a matching manner, and unexpected deviation of the program-controlled cooling scheme on the freezing processing of the stem cell preservation solution caused by excessive or insufficient total storage amount of the stem cell preservation solution is avoided, thereby ensuring that the storage module 200 of the application can cooperate with the acquisition module 100 and the freezing module 300 to realize the freezing preservation of the refined stem cell.
It should be noted that the above-described embodiments are exemplary, and that a person skilled in the art, in light of the present disclosure, may devise various solutions that fall within the scope of the present disclosure and fall within the scope of the present disclosure. It should be understood by those skilled in the art that the present description and drawings are illustrative and not limiting to the claims. The scope of the invention is defined by the claims and their equivalents.
Claims (10)
1. A stem cell harvesting module, characterized in that the harvesting module (100) comprises
A collection unit (101) for obtaining stem cell stock solution, a pretreatment unit (102) for processing the stem cell stock solution into stem cell preservation solution and a quality inspection unit (104) for obtaining stem cell state, wherein,
the quality inspection unit (104) can collect stem cell states of stem cell stock solution collected by the collection unit (101) before entering the pretreatment unit (102), so as to provide references for processing parameter setting and processing parameter adjustment of the pretreatment unit (102).
2. The stem cell harvesting module of claim 1, wherein the stem cell status comprises a first characteristic and a second characteristic, wherein the first characteristic is an endogenous characteristic determined by a characteristic of the stem cell itself and the second characteristic is an exogenous characteristic determined by an environment in which the stem cell is located.
3. The stem cell collection module of claim 2, wherein the first characteristic comprises a class parameter, a structural parameter, and an activity parameter of the stem cell; the second characteristic includes a medium parameter, a temperature parameter, and a flow parameter of the stem cells.
4. The stem cell collection module according to claim 2, wherein the quality inspection unit (104) is further configured to collect stem cell status of a stem cell preservation solution, wherein the stem cell status of the stem cell preservation solution can be used for comparison with a preset freezing condition of the stem cell preservation solution, and the comparison result can be used as a basis for feedback adjustment of processing parameters of the preprocessing unit (102).
5. The stem cell collection module of claim 4, wherein the preset freezing condition is a set threshold or set range for a number of other parameters in the stem cell state based on the category parameters in the stem cell state such that stem cells meeting the set threshold or set range can meet cryopreserved parameter requirements.
6. The stem cell collection module of claim 5, wherein the comparison result is used as a basis for feedback adjustment of the processing parameters of the preprocessing unit (102):
and judging the comparison result based on the comparison result of the parameter value or the parameter range in the stem cell state and the set value or the set range of the threshold condition and the judgment logic of the judgment condition setting so as to obtain a selection adjustment instruction for the processing parameter of the preprocessing unit (102), wherein the selection adjustment instruction is used for selecting or adjusting the processing parameter value or the processing parameter range of the preprocessing unit (102).
7. The stem cell collection module of claim 6, wherein the collection module (100) further comprises a transport unit (103) for transporting the stem cell preservation fluid to a storage module (200) capable of cryopreserving the stem cell preservation fluid, wherein transport parameters of the transport unit (103) are matched to a programmed cooling profile of the storage module (200).
8. The stem cell collection module according to claim 7, wherein the storage module (200) comprises a plurality of storage units (201), wherein the plurality of storage units (201) are respectively provided with a transportation path connected with the transportation unit (103), and the transportation unit (103) can transport the total amount of stem cell preservation liquid based on the storage units (201) corresponding to different directions of the transportation path.
9. A smart module for controlling a stem cell stock solution pretreatment process, characterized in that the smart module (400) is configured to:
and comparing and judging the type parameters, the structure parameters and the activity parameters of the stem cells determined by the characteristics of the stem cells, the medium parameters, the temperature parameters and the flow parameters of the stem cells determined by the environment where the stem cells are positioned with preset freezing conditions in preset rules respectively to obtain a first quality inspection result, and selecting and/or adjusting one or more of the centrifugal rotating speed, the centrifugal time in the centrifugal operation parameters, the substance type and the substance proportioning concentration in the cryoprotectant solution configuration parameters based on the first quality inspection result when the stem cell stock solution is treated as the stem cell preservation solution.
10. The intelligent module for controlling a stem cell stock solution pretreatment process according to claim 9, wherein the preset freezing condition is a set threshold or a set range set for several other parameters in a stem cell state based on a category parameter in the stem cell state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311366713.5A CN117413823A (en) | 2023-07-06 | 2023-07-06 | Stem cell collection module |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310821434.7A CN116548430B (en) | 2023-07-06 | 2023-07-06 | Stem cell collection system |
| CN202311366713.5A CN117413823A (en) | 2023-07-06 | 2023-07-06 | Stem cell collection module |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310821434.7A Division CN116548430B (en) | 2023-07-06 | 2023-07-06 | Stem cell collection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117413823A true CN117413823A (en) | 2024-01-19 |
Family
ID=87496794
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311366713.5A Pending CN117413823A (en) | 2023-07-06 | 2023-07-06 | Stem cell collection module |
| CN202310821434.7A Active CN116548430B (en) | 2023-07-06 | 2023-07-06 | Stem cell collection system |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310821434.7A Active CN116548430B (en) | 2023-07-06 | 2023-07-06 | Stem cell collection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN117413823A (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4327799A (en) * | 1979-06-18 | 1982-05-04 | Helmholtz-Institut Fur Biomedizinische Technik | Process and apparatus for freezing living cells |
| US11259520B2 (en) * | 2016-08-04 | 2022-03-01 | Fanuc Corporation | Stem cell manufacturing system, stem cell information management system, cell transport apparatus, and stem cell frozen storage apparatus |
| CN109943470B (en) * | 2019-03-26 | 2022-06-17 | 深圳市第二人民医院 | Cord blood stem cell collector |
| CN114506882A (en) * | 2020-11-14 | 2022-05-17 | 广东伟创科技开发有限公司 | Automatic add medicine water quality sampler and online water quality monitoring system |
| CN215341164U (en) * | 2021-08-27 | 2021-12-28 | 成都清科生物科技有限公司 | Quality management system of placenta source mesenchymal stem cell preparation |
| CN114159473B (en) * | 2022-02-11 | 2022-05-27 | 美亚宜彬生物科技(北京)有限公司 | Compound preparation for improving male reproductive system stem cells and preparation method thereof |
-
2023
- 2023-07-06 CN CN202311366713.5A patent/CN117413823A/en active Pending
- 2023-07-06 CN CN202310821434.7A patent/CN116548430B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN116548430B (en) | 2023-10-20 |
| CN116548430A (en) | 2023-08-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hafez | Preservation and cryopreservation of gametes and embryos | |
| CN101554152B (en) | Frozen semen diluent and preparation method thereof | |
| CN107751186B (en) | Method for rapidly freezing and reviving cells | |
| WO2019142047A2 (en) | Device and method for freeze drying biological samples | |
| EP3219320B1 (en) | Method and solution for cryopreservation of umbilical cord blood and peripheral blood | |
| CN112674078A (en) | Ultrasonic hepatocyte ice-planting cryopreservation device and cryopreservation method thereof | |
| CN108641999A (en) | A kind of ovary tissue freeze and method for resuscitation | |
| CN116548430B (en) | Stem cell collection system | |
| Onofre et al. | What is the best protocol to cryopreserve immature mouse testicular cell suspensions? | |
| Baert et al. | Cryopreservation of human testicular tissue by isopropyl-controlled slow freezing | |
| CN104818241B (en) | Epinephelus lanceolatus fish-skin skin tissue cell line and its construction method | |
| CN114073249A (en) | Slow quick freezing storage method for human T lymphocytes | |
| CN109329272A (en) | A kind of spermatozoa cryopreservation liquid and its preparation method and application | |
| CN112450206A (en) | Non-programmed cell cryopreservation liquid for direct intravenous use | |
| Gvakharia et al. | A method of successful cryopreservation of small numbers of human spermatozoa. | |
| CN112438252B (en) | Tissue freezing and thawing method suitable for mouse heart tissue single cell sequencing | |
| Yang et al. | Optimization of ethylene glycol concentrations, freezing rates and holding times in liquid nitrogen vapor for cryopreservation of rhesus macaque (Macaca mulatta) Sperm | |
| CN115968862A (en) | Cell cryopreservation liquid and application thereof | |
| Mardenli et al. | The effect of follicle size and cryoprotectants on nuclear maturation and early embryonic development of vitrified-thawed Awassi sheep oocytes (Ovis aries). | |
| CN108812642A (en) | A kind of systems approach and application for preparing placenta tissue according to layer of structure and freezing | |
| CN113068684A (en) | Cryopreservation and recovery method of human umbilical cord mesenchymal stem cells | |
| CN100463963C (en) | Freeze preservating method for domestic pig skin tissue | |
| Kuleshova | Fundamentals and current practice of vitrification | |
| CN111374123B (en) | Use method of neutral amino acid as cell cryoprotectant and application thereof | |
| CN112056305B (en) | Balanced vitrification freezing reagent and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |