CN109937799B

CN109937799B - Cordyceps sinensis mycelium with beautifying function

Info

Publication number: CN109937799B
Application number: CN201910189486.0A
Authority: CN
Inventors: 何寒; 黄秀英
Original assignee: Guangxi Zhuangrentang Biological Technology Co ltd
Current assignee: Guangxi Zhuangrentang Biological Technology Co ltd
Priority date: 2019-03-13
Filing date: 2019-03-13
Publication date: 2021-08-17
Anticipated expiration: 2039-03-13
Also published as: CN109937799A

Abstract

The invention belongs to the technical field of biology, and particularly relates to cordyceps mycelia with functions of beautifying and preparation method thereof. The preparation method of the cordyceps mycelia is simple in process and easy to implement, the prepared cordyceps mycelia contains abundant polysaccharides, proteins, cordycepin, adenosine and other substances, wherein the polysaccharides are up to 12.1%, the proteins are up to 44%, and the cordycepin is up to 5040 mg/kg, so that the cordyceps mycelia have good effects of beautifying and caring skin, can improve the immune function of a human body and the motor capacity of the human body, and have remarkable effects of beautifying and caring skin and health care under the combined action of the high polysaccharides, the high proteins and the high cordycepin.

Description

Cordyceps sinensis mycelium with beautifying function

Technical Field

The invention belongs to the technical field of biology, and particularly relates to a cordyceps sinensis mycelium with a function of beautifying and preparation method thereof.

Background

Cordyceps sinensis, also known as Chinese caterpillar fungus, abbreviated as Cordyceps sinensis. Is a traditional rare Chinese medicinal material, and is formed by parasitizing Cordyceps sinensis of Clavicipitaceae of Hypocreaceae in Hepialus larva in mountain meadow soil, making the larva become stiff, and extracting long rod-shaped stroma from the stiff worm head under proper conditions in summer (i.e. a complex formed by fruiting body of Cordyceps sinensis and stiff worm sclerotium (larva corpse)). It is mainly produced in alpine zone and snowy mountain grassland in China Qinghai, Tibet, Sichuan, Yunnan, Gansu, Guizhou province and autonomous region. Modern researches have shown that Cordyceps sinensis contains about 7% of cordycepic acid, 28.9% of saccharides, about 8.4% of fat, about 25% of protein, and 82.2% of fat is unsaturated fatty acid, and further contains vitamin B12, ergosterol, hexose alcohol, alkaloid, etc. The cordyceps sinensis has high medicinal value and mainly has various effects of regulating an immune system, regulating a central nervous system, resisting tumors, resisting fatigue and the like. Although the growth process of the cordyceps sinensis is simple, the physiology is very complex, the cordyceps sinensis is influenced by varieties, insects, weather, environment and the like and has special requirements, the natural growth is very little, the collection is very difficult, the cordyceps sinensis can only grow in a plateau altitude area due to the fact that the environmental requirements of the cordyceps sinensis growth are harsh and limited by regional conditions, and in addition, the demand of people for the cordyceps sinensis is continuously increased and unreasonably excavated, so that the natural resources of the cordyceps sinensis are gradually reduced. In order to solve the shortage of natural cordyceps sinensis resources, the market demand can be met only by artificially cultivating cordyceps sinensis. Therefore, the development of artificial research, test and cultivation in China makes great progress.

With the continuous and deep research of the technology, a brand new cordyceps product, namely cordyceps mycelia, is obtained by a biological submerged fermentation technology. The cordyceps mycelia are obtained by separating wild cordyceps mycelia, extracting cordyceps fungus monomers, and culturing cordyceps mycelia products by taking the cordyceps fungus monomers as a fungus source by using biological engineering methods such as submerged fermentation and the like. And (3) detecting by related departments: the Cordyceps sinensis mycelia are rich in functional components such as cordycepic acid, cordycepin, SOD enzyme, Cordyceps sinensis polysaccharide and the like, and have the same effective components of Cordyceps sinensis, wherein the contents of crude polysaccharide, Cordyceps sinensis polysaccharide and protein are far higher than those of natural Cordyceps sinensis. According to the analysis and inspection of scientists, the cordyceps mycelia have the same function as cordyceps sinensis: the product can enter lung, liver and kidney channels, has effects of invigorating lung, invigorating kidney, strengthening liver, invigorating qi, replenishing essence, has no adverse side effects, and has effects of improving immunity, relieving fatigue, decomposing fatigue substances in muscle, increasing fatigue tolerance, and improving motor ability. The method for producing the cordyceps sinensis mycelia by using the biological submerged fermentation technology has the characteristics of short production period, simple process, high production efficiency, stability and the like, so the cordyceps sinensis mycelia are effective substitutes of cordyceps sinensis and are the main development direction of artificially cultured cordyceps sinensis products.

At present, the traditional cultivation method of cordyceps mycelia is as follows: 1. preparing nutrient solution, inoculating cordyceps sinensis strain, deep fermenting to produce a large amount of mycelia (balls) in the nutrient solution, concentrating and drying to obtain the cordyceps sinensis mycelia. 2. Taking rice, wheat, corn and the like as culture media, inoculating cordyceps strains, culturing the cordyceps to enable the cordyceps to grow over the culture media, drying and crushing to obtain cordyceps mycelia. In the aspect of culturing cordyceps mycelia, relevant documents are retrieved, for example, Chinese patent application No. 201610480693.8 discloses a method for preparing cordyceps mycelia by taking pitaya peel as a culture medium, the method comprises the steps of taking the pitaya peel as the main raw material to prepare the cordyceps mycelia, inoculating cordyceps strains into the culture medium after sterilizing the culture medium, culturing until mycelia overgrow the culture medium, then drying the culture medium overgrowing with the cordyceps mycelia, and finally crushing to obtain the pitaya peel cordyceps mycelia. By implementing the invention, the dragon fruit peel and the cordyceps are combined into another characteristic combination, namely the dragon fruit peel cordyceps mycelium by using a biotechnology, and the nutrients and the effects of the dragon fruit peel and the cordyceps are integrated, so that the nutrition, the functions and the effects of the dragon fruit peel and the cordyceps are more prominent, and the effects of health care, disease prevention, disease treatment and the like are more obvious. For another example, chinese patent application No. 201611058387.1 discloses a method for preparing cordyceps sinensis mycelia with heat-clearing and detoxifying functions, comprising the following steps: sun-drying mung beans, black beans, red beans and soybeans which are free of plant diseases and insect pests and have normal colors, soaking the mung beans, the black beans, the red beans and the soybeans in 5-7% of cane sugar water for 7-10 hours, airing the mung beans, the black beans, the red beans and the soybeans for 1-2 days, mixing potato powder with 4-5% of glucose water to form potato wet powder, then uniformly mixing the mung beans, the black beans, the red beans, the soybeans and the potato wet powder to obtain a mixture, and sterilizing the mixture to obtain a culture medium; inoculating Cordyceps militaris strains according to aseptic operation, standing at 25-35 deg.C for 1-2d, culturing at relative humidity of 60-65% until the mycelia overgrow the culture medium, and culturing at room temperature in dark to overgrow the mycelia; and (4) airing the hyphae in the step (3) for 1-2 days at the temperature of 30-50 ℃, and crushing the aired culture medium to obtain a finished product.

The mycelium cultured by the above process has simple process and short production period, but the cultured mycelium has low content of nutrient components (cordycepin, cordyceps polysaccharide, protein, etc.) and low efficacy. And the product has poor shape, poor taste and even no taste, and cannot attract the attention of people, so the product has poor popularization effect.

Disclosure of Invention

The invention aims to provide a brand-new cordyceps sinensis mycelium with the function of beautifying and preparation method thereof, the cordyceps sinensis mycelium is prepared by taking oat, soybean, tremella, pigskin, fish scale, shrimp skin, corn and egg as culture media and inoculating cordyceps militaris strains for cultivation; the preparation method is novel, the production process is simple and easy to implement, and the prepared cordyceps mycelia contain abundant substances such as polysaccharide, protein, cordycepin, adenosine and the like, wherein the polysaccharide is up to 12.1%, the protein is up to 44%, and the cordycepin is up to 5040 mg/kg; under the combined action of high polysaccharide, high protein and high cordycepin, the effects of beautifying and caring skin and health care are more obvious.

In order to achieve the purpose, the invention adopts the following technical scheme:

a cordyceps mycelium with beautifying function is prepared by taking oat, soybean, tremella, pigskin, fish scale, shrimp bran, corn and egg as culture media and inoculating cordyceps militaris strain for cultivation; the preparation method comprises the following steps:

(1) respectively collecting oat, soybean, tremella, pigskin, fish scale, dried small shrimp, corn and egg for processing for later use;

(2) the raw materials comprise the following components in percentage by weight: 3-8 parts of oat, 2-6 parts of soybean, 2-6 parts of tremella, 1-3 parts of pigskin, 2-5 parts of fish scales, 1-3 parts of dried small shrimps, 0.5-2 parts of corn and 2-5 parts of eggs are taken according to parts by weight for later use;

(3) preparing a nutrient solution: dissolving sugar and peptone in water according to a weight ratio of clear water to sugar to peptone of 100:12:3 to obtain a nutrient solution;

(4) soaking: placing oat, soybean, corn and fish scales in the nutrient solution and soaking for 3-6 hours;

(5) mixing materials: the oat, the soybean, the corn and the fish scales which absorb the nutrient solution are fished up, then mixed with the tremella, the pigskin and the dried small shrimps, and then mixed with the eggs, and mixed and stirred evenly to obtain a mixed material;

(6) and (3) sterilization: putting the mixed materials into a culture box, and sterilizing according to a conventional method;

(7) inoculation: inoculating cordyceps militaris strains in a sterile environment;

(8) and (3) bacteria cultivation: transferring the inoculated culture material into a culture room, and culturing for 7-10 days at 19-23 ℃ in a dark environment until the hyphae eat the material completely;

(9) color conversion: after the hyphae eat the material, transferring the culture material to another culture room, and culturing for 2-4 days at 20-24 deg.C under visible light until the hyphae turn color;

(10) and (3) finished product: and opening the box cover after the color of the hypha is changed, removing the hypha, continuously culturing until the sporocarp germinates, taking out the culture material after the sporocarp germinates on the material surface, and drying at the temperature of below 80 ℃ to obtain the cordyceps sinensis mycelium finished product.

In the preparation method of the cordyceps sinensis mycelia with the function of beautifying, in the step (1), the collection and processing modes of the oat and the soybeans are as follows: respectively collecting oat and soybean without deterioration and insect damage, and respectively cleaning the oat and the soybean for later use.

In the preparation method of the cordyceps sinensis mycelia with the function of beautifying, the step (1) of the preparation method comprises the following steps of: collecting Tremella without deterioration, insect pest and sulfur, soaking in water to make Tremella absorb enough water, taking out and draining water, and pulverizing into 2-3 cm pieces.

In the preparation method of the cordyceps sinensis mycelium with the function of beautifying, in the step (1), the pigskin is collected and processed in the following mode: collecting non-deteriorated and non-diseased pigskin as a raw material, adding water, boiling for 10 minutes, cooling, scraping dirt and plucking hair, and washing with warm water, wherein a small amount of fat in the pigskin is unnecessary to be removed; then placing the pigskin into a high-pressure cooking pot, and cooking for 30 minutes under the condition that the temperature is 121 ℃; fishing out the pigskin, draining, and finally crushing into blocks of 2-3 cm for later use.

In the preparation method of the cordyceps sinensis mycelia with the function of beautifying, the collection and treatment modes of fish scales in the step (1) are as follows: collecting fish scales without deterioration and impurities as raw materials, washing the fish scales with clear water, then soaking the fish scales in a sodium hydroxide solution with the mass concentration of 1% for 5-6 hours, then rinsing the fish scales with clear water until the clear solution is neutral by using a pH test paper, fishing the fish scales after the fish scales are washed clean, and draining the water for later use.

In the preparation method of the cordyceps sinensis mycelia with the function of beautifying, the collection and treatment modes of the corns in the step (1) are as follows: collecting corn without deterioration and insect pest as raw material, and crushing the corn into 20-30 meshes for later use.

In the preparation method of the cordyceps sinensis mycelium with the function of beautifying, the collection and treatment modes of the dried small shrimps in the step (1) are as follows: collecting the shrimp bran without deterioration and impurities as a raw material, washing the shrimp bran with clear water, and draining the water for later use.

The cordyceps sinensis mycelia with the function of beautifying features are prepared by the method, wherein in the step (3), the sugar is brown sugar, white sugar, rock candy or glucose.

The tremella is fruiting body of fungus of Eumycota, Latin's nameTremellaTremella contains more colloid, can absorb a large amount of water, is dried and strongly shrinks into cutin shape, is hard and crisp, is white or beige, and can restore the original shape after absorbing water. Tremella contains abundant pectin, multiple vitamins, 17 amino acids and glycogen. Tremella contains an important organic phosphorus, and has muscle fatigue relieving effect. Tremella is rich in natural plant colloid, and has effects of nourishing yin, moistening skin, and eliminating facial chloasma and freckle after long-term administration. The active ingredient of acidic polysaccharide substance in the tremella can enhance the immunity of human body, mobilize lymphocytes, strengthen the phagocytic capacity of leucocytes and excite the hematopoietic function of marrow; the Tremella polysaccharide has anti-tumor effect. The dietary fiber in the tremella can help gastrointestinal peristalsis and reduce fat absorption, thereby achieving the effect of losing weight. Tremella is rich in carbohydrate and constitutes important substance of organism; storing and providing thermal energy; energy sources necessary to maintain brain function; regulating fat metabolism; providing dietary fiber; protein is saved; remove toxicity and enhance intestinal function. The tremella is rich in protein and has the function of maintaining potassium-sodium balance; eliminating edema, enhancing immunity, regulating hypertension, relieving anemia, and promoting growth. Tremella is rich in cellulose, has satiety effect, is beneficial for reducing weight, and can stimulate gastrointestinal tract after eating, and can be used for preventing and treating constipation, hemorrhoid, etc. Tremella is rich in carotene, and has effects of maintaining integrity of skin mucosa, forming light sensitive substance in visual cell, promoting growth and development, and preventing congenital deficiency. Maintaining reproductive function, and maintaining and promoting immunity. Tremella is rich in copper, and copper is an indispensable micronutrient for human health, and has important influence on the development and functions of blood, central nervous system and immune system, hair, skin and skeletal tissue, and viscera such as brain, liver and heart. Tremella fuciformis rich in potassiumThe Chinese medicinal composition has the effects of helping to maintain nerve health and normal heartbeat rule, preventing stroke and assisting normal muscle contraction. Has blood pressure lowering effect. The tremella is rich in phosphorus, and has effects of forming skeleton and tooth, promoting growth and repairing body tissue and organ, supplying energy and vigor, and participating in regulation of acid-base balance.

The tremella has the following effects and effects: (1) beautifying and skin care: can enhance the anti-injury capability of skin. Contains a large amount of carotene, and is helpful for maintaining normal functions of skin cells and tissues, stimulating skin metabolism and keeping skin moist and tender. Enhancing skin tension and eliminating wrinkle. Tremella also contains a substance similar to gum arabic, and has good nourishing and antiaging effects on skin cuticle. (2) Nourishing yin, tonifying deficiency, moistening lung and protecting liver (3) help chemotherapy patients: tremella can also enhance the tolerance of tumor patients to radiotherapy and chemotherapy. (4) Hair care: protein-containing foods, which are digested and absorbed by the stomach and intestines to form various amino acids, are essential components for synthesizing hair keratin. (5) Energy supplement: contains carbohydrate and saccharide, and can rapidly provide energy for body. (6) Brain strengthening: the tremella contains phosphorus, is an important component for synthesizing lecithin and cephalin, and can enhance memory, delay brain function decline, inhibit platelet aggregation, and prevent cerebral thrombosis. Calcium is an essential substance essential for brain metabolism. (7) Improve immunity (8) strengthen spleen and reduce blood pressure. (9) Nourishing the heart: protecting heart and myocardial cell, and preventing or relieving palpitation and arrhythmia. (10) Detoxification: is rich in vitamin c, and is helpful for liver to detoxify and clean long-term deposited toxin in human body, and promote health. Increasing activity of immunocyte, and eliminating harmful substances in vivo. (11) Loosening bowel to relieve constipation, reducing weight (12), strengthening bones, inhibiting cancer and resisting tumor; (13) to clear away lung-heat and nourish blood.

The oat (Latin's name: Avena sativaL.) is a gramineous plant, and is called brome and wild wheat in the compendium of materia Medica. Oats, which are not easy to exfoliate, are called oat flakes and are low-sugar, high-nutrient, high-energy foods. Oat is sweet and mild in nature and taste. It has effects in invigorating spleen, nourishing heart, and arresting sweating. Has high nutritive value. The main nutrient components are as follows: phosphorus, iron, calcium, B vitamins, vitamin E, nicotinic acid, folic acid, pantothenic acid and the like. The protein has comprehensive amino acid composition, and 8 kinds of amino acid essential for human body have the first amino acid content, especially contains much lysine. The oat porridge is rich in magnesium and vitamin B1, also contains phosphorus, potassium, iron, pantothenic acid, copper and fiber, can reduce cholesterol, and has auxiliary curative effects on fatty liver, diabetes, constipation and the like. Uncooked oat bran is rich in magnesium, vitamin B1, phosphorus, potassium, and also contains iron, zinc, folic acid, pantothenic acid, and copper. The oatmeal can improve blood circulation and promote wound healing. Oat has medical value and health care function of reducing blood pressure and cholesterol, preventing and treating colorectal cancer and heart diseases, and is generally recognized by medical circles in China and abroad at all times and in China.

The oat contains oat protein, oat peptide, oat beta glucan, oat oil and other ingredients; has antioxidant, skin activity increasing, skin aging delaying, skin whitening, moisture keeping, wrinkle and mottle reducing, and antiallergic effects. Oat contains a large amount of antioxidant substances, such as phenolic acid substances, flavonoid compounds and the like, which can effectively remove free radicals, reduce the damage of the free radicals to skin cells, reduce the appearance of wrinkles, lighten color spots and keep the skin elastic and glossy. The oat beta-glucan contains a large amount of hydrophilic groups, can absorb water or lock the water of a skin stratum corneum, has a very good moisturizing effect, can promote fibroblasts to synthesize collagen, promotes wound healing, and has a good skin repairing function. The oat extract contains a large amount of bioactive components capable of inhibiting tyrosinase activity, the inhibiting capability of the oat extract is close to that of arbutin which is a whitening agent commonly used in cosmetics, but the cost of the oat extract is much lower than that of arbutin. The protein is one of the main components of the oat, small molecular peptides and amino acids can be obtained by enzymolysis of the protein, and the molecules contain hydrophilic groups, can absorb water or lock the water of skin stratum corneum, and have a very good moisturizing effect.

The pigskin is a raw material of meat products with high protein content. The pigskin not only has good toughness and good color, fragrance, taste and mouthfeel, but also has important physiological health-care effect on skin, tendons, bones and hair of people. The protein content of the pigskin is 2.5 times that of pork, the carbohydrate content of the pigskin is 4 times that of the pork, and the fat content of the pigskin is only half of that of the pork. The high-quality fresh pigskin can be comparable with the bear paw, contains rich collagen and elastin, and has the reputation of beauty food. Therefore, the medical and nutritional values of the pigskin can be seen. From the literature, it is known that pigskin has the following efficacy effects: (1) harmonize blood vessels and moisten skin. (2) Wrinkle reduction and beauty treatment: the collagen rich in the pigskin can nourish the skin, can strengthen the absorption and water storage of skin cells, and ensures that the skin is not easy to be shriveled and wrinkled, and becomes smoother, more abundant and full. (3) Delaying senility. The pigskin contains a large amount of collagen which can be converted into gelatin in the cooking process, the gelatin has a reticular space structure, can combine a plurality of water, enhances the physiological metabolism of cells, effectively improves the physiological function of an organism and the water storage function of skin tissue cells, moistens the cells, maintains the moist state and has the function of delaying senility. (4) Improving immunity: the pigskin is rich in protein, carbohydrate and fat, and can supplement part of nutrition required by human body and enhance immunity. (5) And (3) anticancer: scientists find that the pigskin or pig trotter has the functions of delaying aging and resisting cancers when being eaten frequently, and the pigskin contains a large amount of collagen which can slow down the aging of body cells. (6) Nourishing yin, tonifying deficiency, clearing heat, relieving sore throat (7) enriching blood: for the people suffering from anemia, the pigskin can be eaten in a proper amount because of the blood replenishing effect. (8) Adjusting the psychology: the pigskin contains vitamin B12 which can regulate the mind, and the vitamin can improve the mood and improve the phenomenon of dull expression.

The fish scales refer to shells for protecting the fish surfaces and preventing water from losing, and are generally fan-shaped. The fish scale is rich in protein, fat, vitamins, Fe, Zn, Ca, trace elements and colloid. The fish scale also contains various unsaturated fatty acids, and has effects of reducing accumulation of cholesterol on blood vessel wall, and preventing arteriosclerosis, hypertension and heart disease. The research of nutriologists finds that the fish scales are special health-care food. It contains more egg scale fat, and has effects of improving brain memory and delaying cell aging. The fish phosphorus contains abundant protein, fat and multiple vitamins, as well as ferrum, zinc, calcium and multiple essential micronutrients, wherein the content of calcium and phosphorus is very high, and the fish phosphorus can prevent infantile rickets, osteoporosis and fracture of middle-aged and elderly people. Experts find that various unsaturated fatty acids contained in fish scales can help to transfer and emulsify fat in blood in a form of binding protein, reduce cholesterol deposition on blood vessel walls, and have various effects of preventing arteriosclerosis, hypertension, heart disease and the like. The phospholipid bilayer is the framework of the cell, and the lecithin is the unit in the framework, and the proper ingestion of the lecithin can delay the cell aging and promote the repair and regeneration. Fish scales are collagen-generated sclerotin of the dermal layer of fish, known as fish scale scleroprotein, and show remarkable effects in the medical field. The 6-sparse guanine extracted from fish scales is used for clinically treating acute leukemia, and the effective rate is 70-75%; it also has therapeutic effect on gastric cancer and lymphoma. The fish scales contain a large amount of collagen, and compared with other collagen, the fish scale collagen is easy to decompose and digest and absorb. The effect is obvious after people take the medicine, the amino acid in blood can be increased, and the collagen production can be promoted. The fish scale also contains high chitin. Chitin has the following benefits for human health: (1) the immunopotentiation effect of chitin helps to reduce the damage of tumor cells and promote the regeneration and normalization of damaged liver cells. (2) Has no toxic and anticancer effects. (3) Reducing cholesterol, and thus has excellent effects on preventing arteriosclerosis and cardiovascular diseases. (4) Improving digestive function, and preventing constipation. (5) Reducing weight and caring skin. The fish scale food therapy heat is raised from the country by comprehensively observing the fish scale and integrating various nutrients and health-care substances. Fish scales, especially the fish scales of big fish, are good things, and are boiled into jelly, so that the fish scales can supplement calcium, beautify the face, strengthen the body and strengthen the brain and prevent aging when being eaten. However, the fish scales directly collected from the fishes are hard, are not easy to break and are not easy to utilize, and iron, zinc, calcium, a plurality of trace elements necessary for human bodies, chitin and colloid in the fish scales are difficult to separate and dissolve.

The dried small shrimps are food materials and are mainly prepared by processing the prawns. The dried small shrimps have high nutritional value, and the content of protein for measuring the nutritional value is 39.3 g in each 100 g of dried small shrimps, which is greatly higher than that of aquatic products such as large yellow croakers, yellow meal, prawns, hairtails, pomfret and the like and meat products such as beef, pork, chicken and the like. The shrimp skin is rich in iron, calcium and phosphorus, the calcium and phosphorus contents of each 100 g of the shrimp skin are 991 mg and 582 mg, and each 100 g of the shrimp skin contains 39.3 g of protein. The dried small shrimps contain rich protein and mineral substances, particularly contain rich calcium, are called as calcium pool, and are a better way for supplementing calcium for people with calcium deficiency. The dried small shrimps contain rich magnesium which has an important regulating effect on the activity of the heart, can well protect the cardiovascular system, can reduce the content of cholesterol in blood, and has a certain effect on preventing arteriosclerosis, hypertension and myocardial infarction. The dried small shrimps also have a calming effect and are commonly used for treating neurasthenia, vegetative nerve functional disturbance and the like. The old people can eat the dried small shrimps frequently, so that the osteoporosis caused by calcium deficiency can be prevented; the dried small shrimps are put in the meal of the old, which is good for improving appetite and strengthening physique. The dried small shrimps are also characterized by abundant mineral quantity and variety, and besides iodine element which is lack in terrestrial and freshwater organisms, the contents of iron, calcium and phosphorus are also abundant, and the contents of calcium and phosphorus are 991 mg and 582 mg per 100 g of dried small shrimps. Therefore, the prairiin is called "calcium pool". The dried small shrimp actually has astaxanthin which is the strongest antioxidant discovered so far and is called super vitamin E, the content of astaxanthin in the dried small shrimp is higher than that in red, and is about 400-800 ppm. The shrimp bran also contains abundant collagen, so women who love beauty can eat more shrimp bran in daily life to supplement the collagen. The nutrient substances contained in the dried small shrimps are very high, and the dried small shrimps are rich in a large amount of calcium besides collagen, so that experts recommend that a patient with calcium deficiency can drink more dried small shrimp soup in daily life to effectively supplement calcium.

The soybean (with the scientific name of Glycine max (Linn.) Merr.) is generally called soybean, and the soybean is comprehensive in nutrition and rich in content, wherein the content of protein is 2 times higher than that of pork and is 2.5 times higher than that of eggs. The protein content is high and the quality is good. The amino acid composition of the soybean protein is similar to that of animal protein, wherein the amino acid ratio is relatively close to the ratio required by a human body, so that the soybean protein is easy to digest and absorb. Soybean fat also has high nutritive value, and contains many unsaturated fatty acids, and is easily digested and absorbed by human body. In addition, soybean fat can prevent the absorption of cholesterol, so that soybean is an ideal nutrient for patients with arteriosclerosis. Female aging is related to estrogen reduction, while soybean isoflavone, soybean protein, lecithin and the like in soybean milk are well-known natural estrogen supplements. The internal secretion of women can be adjusted by drinking more soybean milk, and the women who insist on drinking the soybean milk every day can obviously improve the mental state and the physical quality, delay aging, maintain beauty and keep young. The nutrients such as selenium, vitamin E, C and the like contained in the soybean milk have great antioxidant function, can make cells of a human body 'rejuvenate', and delay skin aging. In addition, soyasaponin in soybean can scavenge free radicals in vivo, and has antioxidant effect. It also has effects in inhibiting tumor cell growth and enhancing immunity.

The corn (Zea mays L.) is seed of Zea mays L.of Gramineae, and also called corn, pearl rice, etc., and the corn contains abundant cellulose, and not only can stimulate gastrointestinal motility and prevent constipation, but also can promote cholesterol metabolism and accelerate discharge of enterotoxin. The corn has the following effects: (1) weight loss: corn is satiety after eating it, and is not easy to be fat because of its low calorie. (2) Blood pressure and blood fat reduction: the rich calcium can play a role in reducing blood pressure, promoting cell division, reducing serum cholesterol and preventing the cell division from depositing on the blood vessel wall, so that the corn has certain prevention and treatment effects on coronary heart disease, atherosclerosis, hyperlipidemia, hypertension and the like. (3) Memory enhancement and anti-aging: stimulating brain cells, improving human brain, memory and metabolism, and regulating nervous system function. It has effects of making skin tender and smooth, inhibiting and delaying wrinkle generation, and resisting eye aging. (4) Improving eyesight: people who often use eyes should eat more yellow corn to relieve macular degeneration and vision deterioration, and the lutein and zeaxanthin can absorb harmful light entering the eyeball by virtue of strong antioxidation of the lutein and zeaxanthin.

The Cordyceps militaris strain is separated from Cordyceps militaris (Latin's name Cordyceps militaris), the Cordyceps militaris strain used in the invention is obtained by separating fresh and alive grass inoculated and cultured from insect pupae by adopting the prior art, and the strain is subjected to expanded culture before use. Cordyceps militaris is a precious medicinal fungus. Cordyceps militaris and Cordyceps sinensis belong to the same genus and different species. The modern medical research proves that the medicine effect and the pharmacology of the cordyceps sinensis have more medicinal value than those of wild cordyceps sinensis. Cordyceps militaris (scarlet caterpiller fungus) contains a large amount of effective components such as cordycepin, and the special medical and health care functions of cordycepin have attracted high attention of experts at home and abroad, and a plurality of health care products, health care foods, cosmetics and medicines mainly containing cordycepin are put on the market. New drugs synthesized from cordycepin for treating leukemia have also been tried in clinical trials at the entry stage in China. The health-care functional components of the cordyceps militaris (cordyceps militaris) not only contain cordycepin, but also contain cordyceps polysaccharide, which is a human body immunopotentiator recognized by international medicine. The traditional Chinese medicine considers that the cordyceps militaris (cordyceps militaris) has the effects of strengthening body resistance and consolidating constitution, has obvious curative effects on senile chronic bronchitis and pulmonary heart disease, and can improve the detoxifying capability of liver and the antiviral and anti-radiation capability of body. Modern medical research shows that cordycepic acid, namely D-mannitol, is a basic medicine for treating cardiovascular and cerebrovascular diseases and has the effects of scavenging free radicals, dilating blood vessels and reducing blood pressure. The nucleotide has effects of inhibiting blood platelet aggregation, preventing heart and brain thrombosis, eliminating chloasma, senile plaque and acne, resisting aging and wrinkle, caring skin, etc.

The invention has the following advantages:

1. the cordyceps sinensis mycelium with the function of beautifying and maintaining beauty and the preparation method thereof provided by the invention have the advantages of novel method, simple production process, short culture period, easiness in implementation and easiness in large-scale production.

2. The cordyceps mycelia with the beautifying and beauty maintaining functions are prepared by taking oat, soybean, tremella, pigskin, fish scale, dried small shrimp, corn and eggs as culture media and inoculating cordyceps militaris strains for cultivation, the oat, the soybean, the tremella, the pigskin, the fish scale, the dried small shrimp, the corn and the cordyceps mycelia form a unique organic combination through biotechnology, and the prepared cordyceps mycelia simultaneously integrate the nutrients and the action effects of the oat, the soybean, the tremella, the pigskin, the fish scale, the dried small shrimp and the corn, so that the nutrition, the function and the effect of the cordyceps mycelia are more prominent. Oat, soybean, white fungus, pigskin, fish scale and dried small shrimps all contain rich collagen, the invention cultures the cordyceps strain in the culture medium taking oat, soybean, white fungus, pigskin, fish scale, dried small shrimps, corn and eggs as raw materials, the cordyceps strain can absorb the nutrient substances in the substances, the cordyceps polysaccharide, cordyceps adenosine, protein, cordycepin and other substances are promoted to be produced with high yield, the produced cordyceps mycelium product has obvious beautifying function, other health care effects on human body are obvious, and the product has beautiful shape and good taste.

3. According to the cordyceps sinensis mycelium with the beautifying function and the preparation method thereof, provided by the invention, the cordyceps sinensis mycelium is used for fermenting the culture medium, so that the nutrition in the culture medium can be separated early in advance, and meanwhile, the nutrient substances in the culture medium are decomposed in advance, so that the prepared cordyceps sinensis mycelium is easily absorbed after people eat, the absorption rate is improved, and the diseases of the digestive tract are reduced.

4. According to the cordyceps sinensis mycelia with the beautifying function and the preparation method thereof, harmful substances such as residual heavy metals in oat, soybean, tremella, pigskin, fish scale, shrimp bran and corn can be decomposed by fermenting the culture medium with the cordyceps sinensis mycelia, so that the food is safer.

5. The cordyceps sinensis mycelium with the function of beautifying and maintaining beauty and the preparation method thereof provided by the invention have the characteristics of short cultivation period, simple production and preparation process, high production efficiency and stability of products and the like.

6. According to the cordyceps sinensis mycelium with the function of beautifying and maintaining the beauty and the preparation method thereof, the substrate consisting of the oat, the soybean, the tremella, the pigskin, the fish scale, the dried small shrimps and the corn is rich in nutrition, the cultured cordyceps sinensis mycelium contains rich polysaccharide, protein, cordycepin, adenosine and other substances, and the substrate is loose, so that the mycelium can eat materials quickly, and only 7-10 days are needed from inoculation to the completion of the eating of the mycelium.

7. According to the technical scheme, the pigskin contains rich protein, fat, collagen and elastin, after the pigskin is absorbed and converted by hyphae, the fat is completely absent, and the pigskin is taken as one of the raw materials of the culture medium, so that the pigskin can provide rich nutrition for the hyphae and promote the growth of the hyphae.

8. The fish scales are hard in texture, are difficult to eat directly and break, are difficult to absorb by a human body even if being eaten after being crushed, and are generally subjected to calcium removal processing to cause calcium loss; by adopting the technical scheme of the invention, after the fish scales are absorbed and transformed by hypha, the fish scales are effectively decomposed and easily absorbed by people after eating, and the contained calcium can be completely reserved, so that the fish scales are effectively utilized.

9. By adopting the cordyceps sinensis mycelia with the function of beautifying and maintaining beauty and the preparation method thereof, the postnatal appearance of oat, soybean, tremella, pigskin, fish scale, dried small shrimps and corn is effectively solved, the development of related industries of oat, soybean, tremella, pigskin, fish scale, dried small shrimps and corn is driven, a new economic growth point is formed, and great economic benefit and social benefit are achieved. In addition, the invention adopts the pigskin and the fish scales as one of the raw materials of the culture medium for culturing the cordyceps mycelia for the first time, so that the pigskin and the fish scales are fully utilized, the pigskin and the fish scales both contain rich nutrients such as collagen and the like, the growth of mycelia can be promoted, and the contents of polysaccharide, protein and cordycepin in the obtained cordyceps mycelia are high through absorption and conversion of the mycelia.

10. Although there is a method for culturing cordyceps mycelia by taking cereals such as rice, wheat and corn as culture media in the prior art, the cordyceps mycelia obtained by taking the cereals as the culture media are low in cordycepin, cordyceps polysaccharide, protein and the like, and the contents of the components are closely related to the pharmacological and medicinal effects of cordyceps mycelia products, and the cordyceps mycelia prepared by the method are prepared by taking oat, soybean, tremella, pigskin, fish scales, dried small shrimps, corn and eggs as culture media and inoculating cordyceps militaris strains for culturing; the prepared cordyceps mycelia contain abundant polysaccharides, proteins, cordycepin, adenosine and other substances, wherein the polysaccharides are 12.1 percent, the proteins are 44 percent, and the cordycepin is 5040 mg/kg, and the content of functional components of the cordyceps mycelia is higher than that of the cordyceps mycelia produced by the prior art, and the cordyceps militaris sporocarp cultivated by using oat, soybean, tremella, pigskin, fish scales, dried small shrimps, corn and eggs as a culture medium and adding cordyceps militaris strains is utilized, the polysaccharides are 3.3 percent, the cordycepin is 11200 mg/kg, and the adenosine is 1880 mg/kg, so that the cordyceps mycelia have good effects of beautifying and caring skin, the immune function of a human body can be improved, the athletic ability of the body is improved, and the effects of beautifying and caring skin and health are more obvious under the combined action of high polysaccharides, high proteins and high cordycepin.

Drawings

FIG. 1 is a diagram showing the condition that the inoculated culture material is transferred to a culture room for culture in step (8) of the preparation method of cordyceps sinensis mycelia with the function of beautifying;

FIG. 2 is a diagram showing the condition that mycelia are cultured until substantially all of the fruiting bodies germinate on the surface of the culture medium in step (10) of the preparation method of cordyceps mycelia with skin caring function of the present invention.

Detailed Description

The process of the present invention is further illustrated below with reference to examples.

Example 1

the oat and soybean are collected and processed in the following modes: respectively collecting oat and soybean without deterioration and insect damage, and respectively cleaning the oat and the soybean for later use; the tremella is collected and processed in the following modes: collecting Tremella without deterioration, insect pest and sulfur, soaking in water to make Tremella absorb enough water, taking out and draining water, and pulverizing into 2-3 cm pieces;

the pigskin collection and treatment method comprises the following steps: collecting non-deteriorated and non-diseased pigskin as raw material, boiling in water for 10 min, cooling, removing dirt and hair, cleaning with warm water, placing pigskin in high-pressure cooker, and high-pressure steaming at 121 deg.C

Steaming for 30 minutes; fishing out the pigskin, draining, and finally crushing into blocks of 2-3 cm for later use;

the fish scale collecting and processing method comprises the following steps: collecting fish scales without deterioration and impurities as raw materials, washing the fish scales with clear water, then soaking the fish scales in a sodium hydroxide solution with the mass concentration of 1% for 5 hours, then rinsing the fish scales with clear water until the clear solution is neutral as determined by a PH test paper, fishing the fish scales out after the clear solution is washed, and draining the water for later use;

the corn collection and treatment mode is as follows: collecting corn without deterioration and insect pest as raw material, and crushing the corn into 20 meshes for later use; the collection and treatment mode of the dried small shrimps is as follows: collecting the shrimp bran without deterioration and impurities as a raw material, washing the shrimp bran with clear water, and draining the water for later use;

(2) the raw materials comprise the following components in percentage by weight: taking 3 parts of oat, 2 parts of soybean, 2 parts of tremella, 1 part of pigskin, 2 parts of fish scale, 1 part of dried small shrimp, 0.5 part of corn and 2 parts of egg for later use;

(3) preparing a nutrient solution: dissolving brown sugar and peptone in water according to the weight ratio of clear water to brown sugar to peptone of 100:12:3 to obtain a nutrient solution;

(4) soaking: placing oat, soybean, corn and fish scales in the nutrient solution and soaking for 3 hours;

(8) and (3) bacteria cultivation: transferring the inoculated culture material into a culture room, and culturing for 10 days at 19 ℃ in a dark environment until the hyphae eat the material;

(9) color conversion: after the hyphae eat the material, the culture material is moved to another culture room and is cultured for 4 days under the light of 20 ℃ until the hyphae turn color;

Example 2

the pigskin collection and treatment method comprises the following steps: collecting non-deteriorated and non-diseased pigskin as a raw material, adding water, boiling for 10 minutes, cooling, scraping dirt and plucking hair, cleaning with warm water, putting the pigskin into a high-pressure cooking pot, and cooking for 30 minutes at the temperature of 121 ℃ under high pressure; fishing out the pigskin, draining, and finally crushing into blocks of 2-3 cm for later use;

the fish scale collecting and processing method comprises the following steps: collecting fish scales without deterioration and impurities as raw materials, firstly washing the fish scales with clear water, then placing the fish scales in a sodium hydroxide solution with the mass concentration of 1% to soak for 5.5 hours, then rinsing the fish scales with clear water until the clear solution is neutral by using a PH test paper, fishing the fish scales out after the clear solution is rinsed, and draining the water for later use;

the corn collection and treatment mode is as follows: collecting corn without deterioration and insect pest as raw material, and crushing the corn into 25 meshes for later use; the collection and treatment mode of the dried small shrimps is as follows: collecting the shrimp bran without deterioration and impurities as a raw material, washing the shrimp bran with clear water, and draining the water for later use;

(2) the raw materials comprise the following components in percentage by weight: taking 5 parts of oat, 4 parts of soybean, 4 parts of tremella, 2 parts of pigskin, 3 parts of fish scale, 2 parts of dried small shrimp, 1 part of corn and 3 parts of egg according to parts by weight for later use;

(3) preparing a nutrient solution: dissolving white sugar and peptone in water according to a weight ratio of clear water to white sugar to peptone of 100:12:3 to obtain a nutrient solution;

(4) soaking: placing oat, soybean, corn and fish scales in the nutrient solution and soaking for 5 hours;

(8) and (3) bacteria cultivation: transferring the inoculated culture material into a culture room, and culturing for 8 days at 22 ℃ in a dark environment until the hyphae eat the material;

(9) color conversion: after the hyphae eat the material, the culture material is moved to another culture room and is cultured for 3 days under the light of 22 ℃ until the hyphae turn color;

Example 3

the fish scale collecting and processing method comprises the following steps: collecting fish scales without deterioration and impurities as raw materials, washing the fish scales with clear water, then soaking the fish scales in a sodium hydroxide solution with the mass concentration of 1% for 6 hours, then rinsing the fish scales with clear water until the clear solution is neutral as determined by a PH test paper, fishing the fish scales out after the clear solution is washed, and draining the water for later use;

the corn collection and treatment mode is as follows: collecting corn without deterioration and insect pest as raw material, and crushing the corn into 30 meshes for later use. The collection and treatment mode of the dried small shrimps is as follows: collecting the shrimp bran without deterioration and impurities as a raw material, washing the shrimp bran with clear water, and draining the water for later use;

(2) the raw materials comprise the following components in percentage by weight: weighing 8 parts of oat, 6 parts of soybean, 6 parts of tremella, 3 parts of pigskin, 5 parts of fish scale, 3 parts of dried small shrimp, 2 parts of corn and 5 parts of egg according to parts by weight for later use;

(3) preparing a nutrient solution: dissolving crystal sugar/glucose and peptone in water according to the weight ratio of clear water to crystal sugar/glucose to peptone of 100:12:3 to obtain a nutrient solution;

(4) soaking: placing oat, soybean, corn and fish scales in the nutrient solution and soaking for 6 hours;

(8) and (3) bacteria cultivation: transferring the inoculated culture material into a culture room, and culturing for 7 days at 23 ℃ in a dark environment until the hyphae eat the material;

(9) color conversion: after the hyphae eat the material, the culture material is moved to another culture room and is cultured for 2 days under the light of 24 ℃ until the hyphae turn color;

Product detection

The cordyceps sinensis mycelium entrusted provincial detection mechanism with food qualification, which is prepared in the embodiment 2 of the invention and has the functions of beautifying and face-beautifying, is detected, and the detection report result is shown in the following table 1:

the detection results in the above table 1 show that the cordyceps mycelia prepared by the method of the present invention contains abundant polysaccharides, proteins, cordycepin, adenosine, etc., wherein the polysaccharides are up to 12.1%, the proteins are up to 44%, and the cordycepin is up to 5040 mg/kg. Under the combined action of high polysaccharide, high protein and high cordycepin, the effects of beautifying and caring skin and health care are more obvious.

The cordyceps sinensis fruiting body entrusted provincial detection mechanism with food qualification, which is prepared in the embodiment 2 of the invention and has the functions of beautifying and face nourishing, is detected, and the detection report result is shown in the following table 2:

the detection results in the above table 2 show that the cordyceps fruiting body prepared by the method of the invention contains abundant polysaccharides, cordycepin, adenosine and other substances, wherein the polysaccharide content is up to 3.3%, the cordycepin content is up to 11200 mg/kg, and the adenosine content is up to 1880 mg/kg. Under the combined action of high polysaccharide, high cordycepin and high adenosine, the beautifying and health-care effects are more obvious.

Application effects

Application example 1

Grandchild, female, age 27, symptoms: the cordyceps mycelium product produced by the invention is introduced to be taken, after the cordyceps mycelium product is taken for 3 weeks, intestines and stomach are unobstructed, no constipation phenomenon occurs, the conditions of yellow and dark face and roughness are improved, the face becomes glossy, the skin becomes finer and smoother than before, and a lot of acnes and comedo on the face obviously disappear.

Application example 2

Zhao somewhere, woman, age 38, symptoms: the insomnia is serious, the body is lassitude, the limbs are weak, the spirit is poor, the mood is easy to be depressed, and the dysphoria is easy to happen; the cordyceps mycelium product prepared by the invention is introduced to be drunk in soup, so that the metabolism is promoted, the fatigue is eliminated, the physical strength is recovered, the insomnia condition is obviously improved after the cordyceps mycelium product is adhered for 3 weeks, and the melanin is reduced; the skin turns white, the complexion is sallow and yellow, the complexion becomes ruddy, the complexion becomes good, and the mental state becomes good.

Application example 3

Wu-za, male, age 31, symptoms: the cordyceps mycelium product prepared by the invention is introduced to be boiled in soup for drinking, after the cordyceps mycelium product is adhered for 4 weeks, the facial wrinkles are reduced and lightened, the area of the color spot is reduced, the skin becomes body-building, ruddy and glossy, and the cordyceps mycelium product has elasticity compared with the prior cordyceps mycelium product, and the situation of large pores is improved.

Claims

1. A cordyceps sinensis mycelium with the function of beautifying features is characterized in that: the cordyceps mycelia is prepared by taking oat, soybean, tremella, pigskin, fish scales, dried small shrimps, corn and eggs as culture media and inoculating cordyceps militaris strains for cultivation; the preparation method comprises the following steps:

(10) and (3) finished product: opening the box cover after the color of the hypha is changed, removing the hypha, continuously culturing until the sporocarp germinates, taking out the culture material after the sporocarp germinates on the material surface, and drying at the temperature of below 80 ℃ to obtain a cordyceps mycelium finished product;

in the step (1), the tremella is collected and processed in the following way: collecting Tremella without deterioration, insect pest and sulfur, soaking in water to make Tremella absorb enough water, taking out and draining water, and pulverizing into 2-3 cm pieces;

in the step (1), the pigskin is collected and processed in the following mode: collecting non-deteriorated and non-diseased pigskin as a raw material, adding water, boiling for 10 minutes, cooling, scraping dirt and plucking hair, cleaning with warm water, putting the pigskin into a high-pressure cooking pot, and cooking for 30 minutes at the temperature of 121 ℃ under high pressure; fishing out the pigskin, draining, and finally crushing into blocks of 2-3 cm for later use;

in the step (1), the fish scales are collected and processed in the following modes: collecting fish scales without deterioration and impurities as raw materials, washing the fish scales with clear water, then placing the fish scales in a sodium hydroxide solution with the mass concentration of 1% to soak for 5-6 hours, then rinsing the fish scales with clear water until the clear solution is neutral by using a pH test paper, fishing the fish scales out after the clear solution is washed, and draining the water for later use;

in the step (1), the collection and treatment mode of the dried small shrimps is as follows: collecting the shrimp bran without deterioration and impurities as a raw material, washing the shrimp bran with clear water, and draining the water for later use;

in the step (3), the sugar is brown sugar, white sugar, crystal sugar or glucose;

in the step (1), the oat and the soybeans are collected and processed in the following modes: respectively collecting oat and soybean without deterioration and insect damage, and respectively cleaning the oat and the soybean for later use; the corn collection and treatment mode is as follows: collecting corn without deterioration and insect pest as raw material, and crushing the corn into 20-30 meshes for later use.