CN109925335A - The composition of prevention carcinoma of mouth for oral-cavity article - Google Patents
The composition of prevention carcinoma of mouth for oral-cavity article Download PDFInfo
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Abstract
The present invention provides a kind of compositions of prevention carcinoma of mouth for oral-cavity article, the composition includes the component of following parts by weight: 2-15 parts of birch young pilose antlers, 0.01-2.0 parts of borneols, 0.1-5 parts of menthols, 0.01-2.5 parts of boraxs, 0.01-1.5 parts of basil oils, 0.01-25 parts of potassium nitrate, 0.01-15 parts of strontium chlorides and 0.01-10 parts of zinc citrates；Prevention carcinoma of mouth composition is also disclosed in the application in tooth powder, toothpaste and mouthwash.Oral cavity can be effectively nursed provided by the present invention for the composition of the prevention carcinoma of mouth of oral-cavity article; tooth mobility is prevented, protects cell membrane DNA not injured, repair mucous membrane of mouth and the generation of the various symptoms of oral inflammation initiation and prevention mouth cancers, is used for a long time and can achieve the generation for preventing and treating mouth disease effect especially prevention mouth cancers.
Description
Technical field
The invention belongs to oral cavity class product technical fields, and in particular to a kind of group of the prevention carcinoma of mouth for oral-cavity article
Close object.
Background technique
With the promotion of people's living standard, the development of especially modern Preventive dentistry, oral health care aspect
The demand of functional product in terms of product especially prevents carcinoma of mouth has been raised to the column of people's articles for daily use.
Carcinoma of mouth is the malignant tumour general name occurred in oral cavity, including tongue cancer, buccal cancer, carcinoma of floor of mouth, gingival carcinoma, hard palate
Cancer, lip cancer etc. are the more typical malignant tumours of incidence, are the 6th of whole body malignant tumour.Currently, the side for the treatment of carcinoma of mouth
Method has operation excision, radiation cure, chemotherapy etc., although the effect of radiotherapy in the treatment carcinoma of mouth is to be worth affirmative,
Side effect is larger, hypoimmunity, and oral cancer patient is rear poor after treatment, it is difficult to eradicate.
There are two aspects for the main inducing of carcinoma of mouth, first is that oral inflammation, ulcer induce: long-term smoking is drunk, and oral cavity is defended
Raw habit is poor, is bacterium or mould breeds in oral cavity, breeds and create condition, to be conducive to the shape of nitrosamine and its precursor
At.In addition long-term oral inflammation causes some cells to be in proliferative state, and such various reasons may promote induction mucous membrane white
Spot and erythema, canker sore more do not induce canceration for a long time.Second is that mechanical long term oral mucosal irritation induction: root of the tooth is sharp keen
Cusp, inappropriate artificial tooth long-time stimulus mucous membrane of mouth generate chronic stomatocace or even canceration, separately long-term betel quid chewing, and
Arecaline (betel nut is confirmed as a kind of carcinogenic medicine by International Cancer Research Center in 2004) is that the essence of betel nut takes object, it can stimulate glue
The synthesis of former albumen, increase collagen amount, the patient of long-term betel quid chewing, (i.e. so as to cause oral submucosa fibrosis disease
OSF), canceration is further induced.
It is directed to the prevention of oral inflammation, ulcer and carcinoma of mouth at present, is concentrated mainly on improvement oral sanitary habit, changes and inhales
Cigarette, the bad habit drunk and chew betel nut reduce the carcinogenic substances such as tobacco and wine to oral mucosa chemical stimulation, avoid betel quid chewing counterpart
The long term physical of chamber mucous membrane stimulates, and induces oral inflammation, causes the generation of canceration.Good oral sanitary habit is formed as located early
Cacodontia, especially residual crown and root, malposed tooth and sharp keen point of grinding one's teeth in sleep are managed, ill fitting prosthesis is removed, still not from stomatitis
Effective efficiency product development is carried out in terms of disease, ulcer, the prevention of carcinoma of mouth and oral health.
In recent years, since consumer healthcare realizes constantly enhancing, to oral-cavity articles such as functional toothpaste, mouthwash and tooth powder
More requirements are proposed, wherein toothpaste, mouthwash, tooth powder etc. are indispensable oral cleaning health in people's daily life
Articles are that cleaning tooth, fresh breath achieve the purpose that keep oral cleaning, gradually to function from its basic function
It can be expanded in terms of product development, be the market mainstream of domestic and international oral cavity cancerous precaution and adjuvant treatment.Natural plant active at
It many advantages, such as point with its active good, Small side effects, is gradually paid close attention to by vast demander, therefore add natural plant active
The functional oral cavity nursing health products of ingredient are the emphasis of current research and product development concern.
Birch young pilose antler (Inonotus obliquus (Fr) Pilat) is also known as Inonotus obliquus, inonotus obliquus, birch cancer fibre pore fungi etc.,
The ground such as Russia, Poland are a kind of using very extensive medicinal fungi, and principle active component is Fuscoporia obliqua polysaccharide, black
Element, inonotus obliquus, lignin, Inonotus obliquus alcohol etc., pharmacological research be concentrated mainly on antitumor, strengthen immunity,
It is anti AIDS virus, anti-mutation, anti-inflammatory etc., anticancer activity report mainly for breast cancer, liver cancer, gastric cancer, colon cancer
Deng, but have no that it is used for the prevention and treatment of oral inflammation, ulcer and tumour.
Summary of the invention
Aiming at the above shortcomings existing in the prior art, an object of the present invention is to provide a kind of for oral-cavity article
Prevent the composition of carcinoma of mouth, the second object of the present invention is to a kind of tooth powder for preventing mouth cancers is provided, the three of the object of the invention
It is to provide a kind of toothpaste for preventing mouth cancers, the four of the object of the invention are to provide a kind of mouthwash for preventing mouth cancers.
To achieve the above object, the invention provides the following technical scheme: a kind of prevention carcinoma of mouth for oral-cavity article
Composition, the composition include the component of following parts by weight: 2-15 parts of birch young pilose antlers, 0.01-2.0 parts of borneols, 0.1-5 portions of peppermints
Brain, 0.01-2.5 part borax, 0.01-1.5 parts of basil oils, 0.01-25 parts of potassium nitrate, 0.01-15 parts of strontium chlorides and 0.01-
10 parts of zinc citrates.
The birch fine and soft (Inonotus obliquus (Fr) Pilat) is also known as Inonotus obliquus, inonotus obliquus, birch cancer fibre hole
Bacterium etc. is a kind of using very extensive medicinal fungi on Russia, Poland and other places, and principle active component is that Inonotus obliquus is more
Sugar, melanin, inonotus obliquus, lignin, Inonotus obliquus alcohol etc., pharmacological research are concentrated mainly on antitumor, enhancing
It is immunity, anti AIDS virus, anti-mutation, anti-inflammatory etc., anticancer activity report mainly for breast cancer, liver cancer, gastric cancer,
Colon cancer etc. is this time applied to the prevention and treatment of oral inflammation, ulcer and tumour.
The birch fine and soft (Inonotus obliquus (Fr) Pilat) is also known as Inonotus obliquus, inonotus obliquus, birch cancer fibre hole
Bacterium etc. is a kind of using very extensive medicinal fungi on Russia, Poland and other places, and principle active component is that Inonotus obliquus is more
Sugar, melanin, inonotus obliquus, lignin, Inonotus obliquus alcohol etc., pharmacological research are concentrated mainly on antitumor, enhancing
It is immunity, anti AIDS virus, anti-mutation, anti-inflammatory etc., anticancer activity report mainly for breast cancer, liver cancer, gastric cancer,
Colon cancer etc. is this time applied to the prevention and treatment of oral inflammation, ulcer and tumour.
The borneol Borneolum Syntheticum, acrid flavour, hardship are slightly cold；Return heart, liver, lung channel；Delicate fragrance a surname dissipate, tool
There is inducing resuscitation of having one's ideas straightened out, heat-clearing dissipates poison cures mainly aphtha tooth swells, and oral cavity anabrosis, ulcer, aphthae, abscess of throat, especially treatment are burst
Ulcer stomatitis effect is rather good, has good therapeutic effect to certain exedens or stomatocace.Analgesic effect is obvious, accelerates inflammation
It eliminates, oral mucosa is promoted to strip off, rotten to the corn and ulcer healing.Especially mucous membrane caused by physical factor (betel quid chewing) is damaged
Evil works well, and to mucosal injury caused by bacterium or other complicated factors, it can also play auxiliary therapeutic action.It loses to Portugal
Bacterium, streptococcus, Diplococcus pneumopniae, the pathogenic dermatophyte of Escherichia coli and part etc. have inhibiting effect.
The menthol: Menthol has the effect of dispelling wind, heat-clearing.Outer pharyngalgia is controlled, dentalgia has extremely strong sterilization antibacterial
Effect, it is common to prevent mouth disease, it is pre- anti-halitosis, make breath freshening.Menthol can be by adjusting mammalian nervous system
The γ-aminobutyric acid (GABA) and Glycine Receptors of system, are the stereoselectivity regulators of this inhibition ion channel, have
Comparable anti-inflammatory analgesic action.
The borax: borax, it is sweet in flavor, salty, it is cool in nature, it attaches to the lung and stomach meridians.Function is clearing heat and detoxicating, detumescence anti-corrosion, clearing lung-heat
Resolving sputum, primary treatment abscess of throat, aphthae, red eye, swell pain, ocular, phlegm-heat cough.Disappear for oral cavities such as stomatitis, pharyngitis
Malicious anti-corrosion.
The basil oil: Oclmum gratissimum oil, Pharmacopoeia of the People's Republic of China version in 2010,
It is mainly used for topical pain relief, antiseptic, dental drugs, spices and essence is used.
The potassium nitrate is a kind of desensitizer, and desensitization effect is good and lasting, does not stimulate gum, does not make tooth discoloration, even
You swallow also non-toxic.The strontium chloride is that strontium ion has to antianaphylactic effect, and slight hypersensitive dentin can play one
Fixed relaxation effect.The zinc citrate has positive effect to removal tartar and calculus dentalis.
The birch young pilose antler is one or both of birch young pilose antler powder or birch young pilose antler extract；Wherein, birch young pilose antler powder can pass through
Powder acquisition is directly played after birch young pilose antler is dry, or birch young pilose antler is pulverized using air-flow disintegrating machine, impalpable powder is made；The birch
It sets fine and soft extract to extract by water and/or alcohol extracting or water extract-alcohol precipitation or alcohol extracting-water precipitating, then purified, be concentrated, powder processed obtains birch
Fine and soft extract.
A kind of composition answering in tooth powder, toothpaste and mouthwash for the prevention carcinoma of mouth being previously described for oral-cavity article
With.
The present invention also provides a kind of tooth powder for preventing mouth cancers, and in parts by weight, the auxiliary material of the tooth powder includes 35-
70 parts of rubbing agents, 1.5-2.5 parts of foaming agents, 0.15-0.5 parts of stabilizers, 0.1-1.5 portions of preservatives, 0.25-0.35 parts of sweeteners
It further include the composition of 2.5-68 parts of above-mentioned prevention carcinoma of mouth with 0.8-1.5 parts of edible essences.
Further, the rubbing agent is that hydrated silica or calcium carbonate or hydrated silica and calcium carbonate are living with biology respectively
Property glass or hydroxyapatite or calcium monohydrogen phosphate combination of two；Rubbing agent wipes dental surface tartar with toothbrush collective effect
The solid material ingredient of equal foreign substances；The foaming agent is laruyl alcohol sodium sulfovinate or sodium N-lauroyl sarcosinate or lauroyl
One or both of sodium glutamate；The addition of foaming agent is that have good so that toothpaste can reduce surface tension of liquid
Wetting, foaming, emulsification, detergent action；The stabilizer is sodium pyrophosphate or DETA disodium；When magnesium and doped calcium are on tooth
It will form winestone, and stabilizer can eliminate winestone, to achieve the effect that clean tooth；The preservative is o- cymene -5-
Alcohol or western pyrrole chloramines or one or both of antibacterial peptide or grape fruit extract；The sweetener be saccharin sodium or xylitol or
Sucralose or stevia.
The present invention also provides a kind of toothpaste for preventing mouth cancers, and in parts by weight, the auxiliary material of the toothpaste includes power
Benefit requires the auxiliary material of tooth powder and 20-50 parts of wetting agents, 0.8-1.5 parts of adhesives and 20-80 parts of deionized waters in 5, further includes
The composition of 3.5-68 parts of above-mentioned prevention carcinoma of mouth.
Further, the wetting agent is two or three in glycerol or propylene glycol or polyethylene glycol or sorbierite, wetting
The addition of agent can stablize the moisture in toothpaste, keep the plasticity of toothpaste and make its softness lubrication.Described adhesive is carboxylic first
Base sodium cellulosate or hydroxyethyl cellulose or xanthan gum or one or both of melon Lovell glue or carragheen；Adhesive can divide
It dissipates, swell in toothpaste liquid phase, stable colloid is formed, to be suspended in toothpaste solid phase surface, to prevent solid phase and liquid phase in toothpaste
Component separation.
The present invention also provides a kind of mouthwashes for preventing mouth cancers, with parts by weight agent, the accessory package of the mouthwash
Include 1.5-2.5 parts of foaming agents, 0.15-0.5 parts of stabilizers, 0.1-1.5 portions of preservatives, 0.25-0.35 parts of sweeteners, 0.8-1.5
Part edible essence, 20-50 parts of wetting agents and 20-80 parts of deionized waters further include the groups of 2.5-67 parts of above-mentioned prevention carcinoma of mouth
Close object.
Further, the foaming agent is in laruyl alcohol sodium sulfovinate or sodium N-lauroyl sarcosinate or sodium lauroyl glutamate
One or two；The stabilizer is sodium pyrophosphate or DETA disodium；The preservative is o- cymene -5- alcohol or western pyrrole chlorine
One or both of amine or antibacterial peptide or grape fruit extract；The sweetener be saccharin sodium or xylitol or Sucralose or
Stevia；The wetting agent is two or three in glycerol or propylene glycol or polyethylene glycol or sorbierite.
It is compared with the prior art, the invention has the following beneficial effects:
Provided by the present invention for oral-cavity article prevention carcinoma of mouth composition, due in ingredient birch young pilose antler, borneol,
The collaboration compatibility effect of menthol, borax and basil oil, so that there is composition mitigation swelling and aching of gum and toothache, heat-clearing to go
Fire removes the effect of oral inflammation and repairing ulcer face；Meanwhile oral cavity can be effectively nursed, prevent tooth mobility, protection cell membrane
DNA is not injured, and various symptoms and the generation for preventing mouth cancers that mucous membrane of mouth and oral inflammation cause are repaired；In ingredient
The collaboration compatibility of potassium nitrate, strontium chloride and zinc citrate can play eliminate bad breath, fresh breath, the formation for preventing plaque, together
When play the role of preferable desensitization effect, long-time service, which can achieve, to be prevented and treated mouth disease and especially prevents mouth cancers
Generation, can apply in other oral-cavity articles such as tooth powder, toothpaste, mouthwash.
Specific embodiment
Combined with specific embodiments below, the method for the present invention is described in detail.
One, a kind of composition of the prevention carcinoma of mouth for oral-cavity article
Embodiment 1
A kind of composition of the prevention carcinoma of mouth for oral-cavity article, the ingredient are as shown in table 1 below:
Composition components formula of the table 1 for the prevention carcinoma of mouth of oral-cavity article
Embodiment 2
A kind of composition of the prevention carcinoma of mouth for oral-cavity article, the ingredient are as shown in table 2 below:
Composition components formula of the table 2 for the prevention carcinoma of mouth of oral-cavity article
Embodiment 3
A kind of composition of the prevention carcinoma of mouth for oral-cavity article, the ingredient are as shown in table 3 below:
Composition components formula of the table 3 for the prevention carcinoma of mouth of oral-cavity article
Embodiment 4
A kind of composition of the prevention carcinoma of mouth for oral-cavity article, the ingredient are as shown in table 4 below:
Composition components formula of the table 4 for the prevention carcinoma of mouth of oral-cavity article
Embodiment 5
A kind of composition of the prevention carcinoma of mouth for oral-cavity article, the ingredient are as shown in table 5 below:
Composition components formula of the table 5 for the prevention carcinoma of mouth of oral-cavity article
Embodiment 6
A kind of composition of the prevention carcinoma of mouth for oral-cavity article, the ingredient are as shown in table 6 below:
Composition components formula of the table 6 for the prevention carcinoma of mouth of oral-cavity article
Two, a kind of tooth powder for preventing mouth cancers
Embodiment 7
A kind of tooth powder preventing mouth cancers, the ingredient are as shown in table 7 below:
The tooth powder component prescription of the prevention mouth cancers of table 7
The tooth powder of above-mentioned prevention mouth cancers is using following preparation method:
(1) borneol, menthol are added in basil oil and edible essence, and are heated to 30-55 DEG C of stirring, formed
Mixture A；
(2) the birch young pilose antler powder in carcinoma of mouth composition, birch young pilose antler extract, borax, potassium nitrate, strontium chloride, lemon will be prevented
Lemon acid zinc is mixed with rubbing agent, forms mixture B；
(3) A is uniformly sprinkled into B, is stirred evenly, staticaccelerator adsorption 12-24h forms mixture C；
(4) the remaining other compositions in formula are added at one time in mixing C, are stirred evenly, it is canned, tooth powder is made.
Embodiment 8
A kind of tooth powder preventing mouth cancers, the ingredient are as shown in table 8 below:
The tooth powder component prescription of the prevention mouth cancers of table 8
Embodiment 9
A kind of tooth powder preventing mouth cancers, the ingredient are as shown in table 9 below:
The tooth powder component prescription of the prevention mouth cancers of table 9
Embodiment 10
A kind of tooth powder preventing mouth cancers, the ingredient are as shown in the following table 10:
The tooth powder component prescription of the prevention mouth cancers of table 10
Embodiment 11
A kind of tooth powder preventing mouth cancers, the ingredient are as shown in table 11 below:
The tooth powder component prescription of the prevention mouth cancers of table 11
Embodiment 12
A kind of tooth powder preventing mouth cancers, the ingredient are as shown in table 12 below:
The tooth powder component prescription of the prevention mouth cancers of table 12
The preparation method and embodiment 1 of the tooth powder of the prevention mouth cancers of embodiment 8-12 are consistent, are existed on ingredient
Difference.Three, a kind of toothpaste for preventing mouth cancers
Embodiment 13
A kind of toothpaste preventing mouth cancers, the ingredient are as shown in table 13 below:
The toothpaste component formula of the prevention mouth cancers of table 13
The toothpaste of above-mentioned prevention mouth cancers uses following steps:
(1) above-mentioned borneol, menthol are added in basil oil and edible essence, are heated to stirring at 30-55 DEG C,
And closed placement 6-24h, form mixture A；
(2) above-mentioned foaming agent, wetting agent and adhesive are dissolved in formation mixture B in deionized water；
(3) will prevent carcinoma of mouth composition in borax, potassium nitrate, strontium chloride, zinc citrate, stabilizer, sweetener and
Preservative is added in mixture B and stirs evenly, and forms mixture C；
(4) it disperses birch young pilose antler in rubbing agent, forms mixture D；
(5) said mixture C and mixture D are added in emulsion pot, emulsify 20-40min, it is evacuated to -0.096~-
5-20min is stirred after 0.090MPa, is eventually adding mixture A, continues to emulsify 20-60min, it is evacuated to -0.096~-
10-25min is stirred after 0.090MPa, and lotion is made；
(6) above-mentioned lotion is squeezed into the lotion storage pot that temperature is 30-55 DEG C to be aged, digestion time 120-
140min, subsequent cooled to room temperature to get arrive toothpaste.
Wherein, it when preservative contains o- cymene -5- alcohol, needs first to be dissolved o- cymene -5- alcohol with wetting agent, and
Other preservatives can be added directly into mixture B.
Embodiment 14
A kind of toothpaste preventing mouth cancers, the ingredient are as shown in table 14 below:
The toothpaste component formula of the prevention mouth cancers of table 14
Embodiment 15
A kind of toothpaste preventing mouth cancers, the ingredient are as shown in table 15 below:
The toothpaste component formula of the prevention mouth cancers of table 15
Embodiment 16
A kind of toothpaste preventing mouth cancers, the ingredient are as shown in table 16 below:
The toothpaste component formula of the prevention mouth cancers of table 16
Embodiment 17
A kind of toothpaste preventing mouth cancers, the ingredient are as shown in table 17 below:
The toothpaste component formula of the prevention mouth cancers of table 17
Embodiment 18
A kind of toothpaste preventing mouth cancers, the ingredient are as shown in table 18 below:
The toothpaste component formula of the prevention mouth cancers of table 18
The preparation method that embodiment 14-18 prevents the toothpaste of mouth cancers is consistent with the preparation method of embodiment 12, only
It is had differences on ingredient.
Four, a kind of mouthwash for preventing mouth cancers
Embodiment 19
A kind of mouthwash preventing mouth cancers, the ingredient are as shown in table 19 below:
The mouthwash component prescription of the prevention mouth cancers of table 19
The mouthwash of above-mentioned prevention mouth cancers is using following preparation method:
(1) borneol, menthol are mixed with basil oil and essence, is heated to 30-55 DEG C of stirring and dissolving, form mixing
Object A；
(3) wetting agent is added while stirring into mixture A, forms mixture B；
(4) it will be added in mixture B after a small amount of deionized water dissolving of foaming agent, form mixture C；
(5) the birch young pilose antler in carcinoma of mouth composition will be prevented, borax, potassium nitrate, strontium chloride, zinc citrate, stabilizer, prevented
Rotten agent, sweetener utilize the dissolution of remaining water, mixing；Mixture C is added in the mixture, is added mixed to 30-55 DEG C of stirring
It is even, it is static, it is cooled to room temperature, tank bottle is to get mouthwash.
Wherein, it when preservative contains o- cymene -5- alcohol, needs first to be dissolved o- cymene -5- alcohol with wetting agent, and
Other preservatives can directly adopt deionized water dissolving.
Embodiment 20
A kind of mouthwash preventing mouth cancers, the ingredient are as shown in table 20 below:
The mouthwash component prescription of the prevention mouth cancers of table 20
Embodiment 21
A kind of mouthwash preventing mouth cancers, the ingredient are as shown in table 21 below:
The mouthwash component prescription of the prevention mouth cancers of table 21
Embodiment 22
A kind of mouthwash preventing mouth cancers, the ingredient are as shown in table 22 below:
The mouthwash component prescription of the prevention mouth cancers of table 22
Embodiment 23
A kind of mouthwash preventing mouth cancers, the ingredient are as shown in table 23 below:
The mouthwash component prescription of the prevention mouth cancers of table 23
Embodiment 24
A kind of mouthwash preventing mouth cancers, the ingredient are as shown in table 24 below:
The mouthwash component prescription of the prevention mouth cancers of table 24
Embodiment 25
A kind of mouthwash preventing mouth cancers, the ingredient are as shown in table 25 below:
The mouthwash component prescription of the prevention mouth cancers of table 25
The preparation method and embodiment 19 of the tooth powder of the prevention mouth cancers of embodiment 20-25 are consistent, are deposited on ingredient
In difference.
Five, pharmacodynamic test
Carcinoma of mouth is the general name occurred in the malignant tumour in oral cavity, largely belongs to dermoid cancer, i.e., so-called
Mucous membrane morphs.Oral squamous cell carcinoma (oral squamos cell carcinoma, OSCC) endangers human health
One of common cancer, squamous cell carcinoma abbreviation squamous carcinoma also known as epidermal carcinoma are the one kind for betiding epidermis or appendicle cell
Malignant tumour, cancer cell have different degrees of angling, the position of scaly epithelium covering are more common in, such as skin, oral cavity, lip, food
Pipe, cervix, vagina etc..Therefore, pharmacodynamic test of the invention, selection identical tumour cell palace with oral squamous cell carcinoma
Carcinoma of neck cell and Wistar rat close with Human Oral Cavity cancer and physiology of oral cavity environment are as experimental vehicle.
1. utilizing human cervical cancer 1 squamous cell carcinoma SiHa, investigate what present invention prevention carcinoma of mouth composition was proliferated squamous cell carcinoma
Inhibiting effect
1, materials and methods
1.1 material
(1) tumor strain: human cervical cancer 1 squamous cell carcinoma SiHa
(2) experiment reagent: fetal calf serum, RPMI-1640 culture solution, MTT solution etc.；
(3) laboratory sample: according to example 1 group close object formula, using RPMI-1640 complete culture solution by weight into
Row dilution.
1.2 method
(1) cell culture
SiHa cell culture is in the RPMI-1640 culture solution containing 10% fetal calf serum, in 37 DEG C of constant temperature, the training of 5%CO2
Culture in case is supported, 2~3d had digestive transfer culture 1 time, logarithmic growth phase cell is for testing.
(2) MTT experiment
Cell is made 2.0 × 10 with RPMI-1640 complete culture solution496 well culture plates are added in the cell suspension of a/ml
Interior, 180ul is added in every hole, is subsequently placed in cell incubator after culture 14h, is separately added into the laboratory sample of various concentration
20ul makes laboratory sample ultimate density 26.1mg/ml, 5.22mg/ml, 2.61mg/ml, 1.305mg/ml, 0.625mg/ml5
A concentration, while negative control group (20ulRPMI-1640 complete culture solution) and blank control group (180ulRPMI-1640 are set
Complete culture solution+20ul physiological saline), every group is all provided with 5 parallel holes, after cultivating 48h, observes drug effect under inverted microscope
To the influence of its form after cell, 20ul 5mg/ml MTT solution is added in every hole, is put into incubator and is continued to cultivate 4h, every hole
150ulDMSO is added, is protected from light on isothermal vibration device (30 DEG C) and shakes up 10min, microplate reader 492 measures each hole OD value (OD),
Cell inhibitory rate is calculated by formula: inhibiting rate (%)=(negative control group OD value-experimental group OD value)/(negative control group OD value-
Blank control group OD value) × 100%.
2, result
The prevention carcinoma of mouth composition of various concentration is detailed in down the growth inhibition effect of human cervical cancer 1 squamous cell carcinoma SiHa
Table 26.
26 present invention prevention carcinoma of mouth composition of table is to human cervical carcinoma cell SiHa growth inhibition ratio (%) (x ± s)
Using SPSS17.0 statistical software, calculates average value and add and subtract standard deviation.Prevention oral cavity of the invention as shown in Table 26
Cancer composition has certain inhibiting effect to human cervical cancer 1 squamous cell carcinoma SiHa growth, and concentration dependent is presented.
2. being tested using 4-NQO induced rat palatine mucosa canceration model, observes tooth powder, toothpaste and mouthwash of the present invention and inhibit
4-NQO carcinogenic effect.
1, materials and methods
1.1 material
(1) experimental animal: Wistar rat 192,200~220g of weight, age of mouse 10 weeks, 18~25 DEG C of room temperature, humidity
40-50%, standard solid pellet are raised under the conditions of 24 hours natural Dark-light cycles.
(2) experiment reagent: carcinogen: 0.02% strength solution of volume fraction is made with distilled water in 4-NQO (Sigma), and 4
It DEG C is kept in dark place spare；
(3) laboratory sample: the mass ratio of tooth powder (sample of the formula preparation of embodiment 12) and water is 3:1 as laboratory sample
1；Toothpaste (sample of the formula preparation of embodiment 17) is laboratory sample 2；Mouthwash (sample of the formula preparation of embodiment 23) is real
Test sample 3.It certainly, can also be with as long as in the scope of the present invention according to sample prepared by other embodiments of the invention
It is feasible, then this does not just repeat one by one.
1.2 method
(1) experimental group:
192 three groups of Wistar rats point, blank control group: 8；Laboratory sample control group: totally 3 groups, every group each 8；
Canceration model group: 40；Laboratory sample group: totally 3 groups, every group each 40.
Canceration model group smears the viscous of Wistar rat palatine using carcinogen (volume fraction 0.02%4-NQO aqueous solution)
Film is smeared once for two days, and the time is the morning, smears no less than 1min for every every time；Blank control group is smeared with distilled water daily
The mucous membrane of Wistar rat palatine is primary, smears no less than 1min for every every time；Laboratory sample control group, it is real with 1,2,3 daily
It tests sample and smears the mucous membrane of each corresponding laboratory sample control group Wistar rat palatine respectively three times, every minor tick is many
2h smears no less than 1min for every every time；3 groups are smeared a carcinogen for laboratory sample group two days in the viscous of Wistar rat palatine
At film, no less than 1min is smeared for every every time, meanwhile, daily afternoon smears each corresponding reality using laboratory sample 1,2,3 respectively
It is primary to test group mucous membrane of Wistar rat palatine, smears no less than 1min for every every time, applies the time of carcinogen and laboratory sample
It is spaced many 2h.With the extension of coating time, mobility reduction, diet, drinking-water and weight also reducing model group rats,
Part occurs apathetic at 26 weeks, and hair color is dry.
It is random at 14,18,22,26,30 weeks respectively to put to death canceration model group and 3 groups of experimental groups every group of rat each 8 respectively,
Solution takes palate mucous film preparation Histological section, micro- sem observation, blank control group and laboratory sample control group are in 30 Zhou Shiyi
Secondary property is put to death, and solution takes palate mucous film preparation Histological section, micro- sem observation.
(2) dietary amount and weight for recording rat in Germicidal efficacy experimentation weekly, observe and record oral cavity every two weeks
Mucous membrane metamorphosis.Lesion number of cases, pathology are recorded to the rat palatine mucosal tissue pathological section pathology qualification result of execution
It learns to change and is divided into epithelium Simple hyperplasia, slight, moderate, severe abnormality hyperplasia, carcinoma in situ and infiltrating carcinoma, epithelial dysplasia
Diagnosis is referring to WHO2005 standard.
2, result
2.1 gross examination of skeletal muscle
Control group and 3 groups of experimental group each group rat palatine mucosas are smooth, ruddy, flexible；3 groups of experimental groups, 11 rat experiments
First 3 weeks when there is anorexia, weight loss phenomenon, but the phenomenon disappears after 7 weeks, restores normal；Model group is smearing medicine 10
6 rat palate rear portion mucous membranes of Zhou Shiyou bleach, and the position range that bleaches for 14 weeks increases, and the position to bleach starts to thicken, 22 weeks time-varying
There is irregular protrusion in white position, has within 24 weeks 8 positions thickened patch and slight ulcer occur, 9 rat palates at 26 weeks
There is grain granular solid matter not of uniform size and with erosion in rear portion, whole rat palatine fold level disorder at 30 weeks, mucosal hyperplasia,
There is wellability lump in ulcer, partial rat.
2.2 histological observation
All diagnosis are all made of double-blind study confirmation, control group and experimental group do not occur epithelium Simple hyperplasia, it is slight, in
The pathological changes such as degree, severe abnormality hyperplasia, carcinoma in situ and infiltrating carcinoma, see Table 2 for details 7. for the pathological change of model group
The pathological change of 27 model group different time sections rat palatine of table
Show that the pathological change distribution number of cases difference of each period all has statistics through Kruskal-Wallis rank sum test
Learn difference P < 0.01.
3, conclusion
From gross examination of skeletal muscle and histological observation, laboratory sample control group is closely same with blank control group observation result, explanation
Tooth powder, toothpaste and mouthwash are configured to by the composition of prevention mouth cancers and do not show any toxicity reaction, experimental group
In addition to anorexia, weight loss occur in all 11 rats of gross examination of skeletal muscle 3 to 7, remaining time is normal；And model group is seen from histology
It examines the results show that the model is set up.Thus illustrate, the composition that the present invention prevents mouth cancers is configured to tooth powder, toothpaste and gargles
Saliva, which all has, blocks having an effect for mouth cancers, and has no any toxicity reaction.
3. test tooth powder, toothpaste and mouthwash of the present invention to gingivitis, periodontal disease, dental hypersensitivity improvement rate
First group of blank control group (physiological saline)；Second group of tooth powder group (sample of the formula preparation of embodiment 12)；Third
Group toothpaste group (sample of the formula preparation of embodiment 17)；4th group of mouthwash group (sample of the formula preparation of embodiment 23), test
It the results are shown in Table 28.
28 test specimen of table test gingivitis, periodontal disease, dental hypersensitivity patient test result
From the test result of table 28 it is found that blank control group, gingivitis, periodontal disease, dental hypersensitivity patient it is good
Rate of rotation is all very low, and other three groups 80% or more, thus illustrates, the composition that the present invention prevents mouth cancers is configured to
Tooth powder, toothpaste and mouthwash also have effects that prevent and treat gingivitis, periodontal disease, dental hypersensitivity.Prevention and treatment can be thus achieved
Inducement gingivitis, the periodontal disease of mouth cancers generation, and the dental hypersensitivity of later period initiation can be prevented and treated.
To sum up pharmacodynamic test as it can be seen that the present invention prevent carcinoma of mouth composition in oral-cavity article application be safely, have
Effect, it safe can be applied in oral-cavity article.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
Claims (10)
1. a kind of composition of the prevention carcinoma of mouth for oral-cavity article, which is characterized in that the composition includes following parts by weight
Several component: 2-15 parts of birch young pilose antlers, 0.01-2.0 parts of borneols, 0.1-5 parts of menthols, 0.01-2.5 parts of boraxs, 0.01-1.5 parts
Basil oil, 0.01-25 part potassium nitrate, 0.01-15 parts of strontium chlorides and 0.01-10 parts of zinc citrates.
2. the composition of the prevention carcinoma of mouth according to claim 1 for oral-cavity article, which is characterized in that the composition
Component including following parts by weight: 5-10 parts of birch young pilose antlers, 0.5-1.5 parts of borneols, 0.2-3 parts of menthols, 0.1-1.5 parts of boron
Sand, 0.7-1.2 part basil oil, 2-15 parts of potassium nitrate, 2.5-8 parts of strontium chlorides and 1-7 parts of zinc citrates.
3. the composition of the prevention carcinoma of mouth according to claim 2 for oral-cavity article, which is characterized in that the birch
Young pilose antler is one or both of birch young pilose antler powder or birch young pilose antler extract；The birch young pilose antler extract is extracted by water and/or alcohol extracting
Or water extract-alcohol precipitation or alcohol extracting-water precipitating, then it is purified, concentration, powder processed obtains birch young pilose antler extract.
4. it is according to claim 1 to 3 for oral-cavity article prevention carcinoma of mouth composition tooth powder, toothpaste and
Application in mouthwash.
5. a kind of tooth powder for preventing mouth cancers, in parts by weight, the auxiliary material of the tooth powder include 35-70 parts of rubbing agents, 1.5-
2.5 parts of foaming agents, 0.15-0.5 parts of stabilizers, 0.1-1.5 portions of preservatives, 0.25-0.35 parts of sweeteners and 0.8-1.5 parts are edible
Essence, which is characterized in that further include the composition of any prevention carcinoma of mouth of 2.5-68 parts of claim 1-3.
6. it is according to claim 5 prevention mouth cancers tooth powder, which is characterized in that the rubbing agent be hydrated silica or
Calcium carbonate or hydrated silica and calcium carbonate respectively with bioactivity glass or hydroxyapatite or calcium monohydrogen phosphate combination of two；
The foaming agent is laruyl alcohol sodium sulfovinate or one or both of sodium N-lauroyl sarcosinate or sodium lauroyl glutamate；Institute
Stating stabilizer is sodium pyrophosphate or DETA disodium；The preservative is o- cymene -5- alcohol or western pyrrole chloramines or antibacterial peptide or Portugal
One or both of grape shaddock extract；The sweetener is saccharin sodium or xylitol or Sucralose or stevia.
7. a kind of toothpaste for preventing mouth cancers, in parts by weight, the auxiliary material of the toothpaste include the auxiliary of tooth powder in claim 5
Material and 20-50 parts of wetting agents, 0.8-1.5 parts of adhesives and 20-80 parts of deionized waters, which is characterized in that further include 3.5-68
The composition of any prevention carcinoma of mouth of part claim 1-3.
8. the toothpaste of prevention mouth cancers according to claim 6, which is characterized in that the wetting agent is glycerol or the third two
Two or three in alcohol or polyethylene glycol or sorbierite；Described adhesive is sodium carboxymethylcellulose or hydroxyethyl cellulose
Or xanthan gum or one or both of melon Lovell glue or carragheen.
9. a kind of mouthwash for preventing mouth cancers, with parts by weight agent, the auxiliary material of the mouthwash includes 1.5-2.5 parts of foaming
Agent, 0.15-0.5 parts of stabilizers, 0.1-1.5 portions of preservatives, 0.25-0.35 parts of sweeteners, 0.8-1.5 parts of edible essences, 20-50
Part wetting agent and 20-80 parts of deionized waters, which is characterized in that further include any prevention of 2.5-67 parts of claim 1-3
The composition of carcinoma of mouth.
10. the mouthwash of prevention mouth cancers according to claim 9, which is characterized in that the foaming agent is laruyl alcohol
Sodium sulfovinate or one or both of sodium N-lauroyl sarcosinate or sodium lauroyl glutamate；The stabilizer is sodium pyrophosphate
Or DETA disodium；The preservative is o- cymene -5- alcohol or western pyrrole chloramines or one of antibacterial peptide or grape fruit extract
Or two kinds；The sweetener is saccharin sodium or xylitol or Sucralose or stevia；The wetting agent is glycerol or the third two
Two or three in alcohol or polyethylene glycol or sorbierite.
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| CN113564224A (en) * | 2020-04-13 | 2021-10-29 | 台达电子工业股份有限公司 | Biomarkers for malignant transformation of submucosal fibrosis in oral cavity |
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