CN109923971A - A method of promote tsaoko seed to sprout - Google Patents
A method of promote tsaoko seed to sprout Download PDFInfo
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- CN109923971A CN109923971A CN201810413423.4A CN201810413423A CN109923971A CN 109923971 A CN109923971 A CN 109923971A CN 201810413423 A CN201810413423 A CN 201810413423A CN 109923971 A CN109923971 A CN 109923971A
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- tsaoko
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Abstract
The invention discloses a kind of methods that promotion tsaoko seed is sprouted, comprising the following steps: tsaoko fruit is placed in room temperature in ventilative Polywoven Bag and stacked 2-3 days, stripped outside rind, obtain seed group by the tsaoko fruit of acquisition fresh mature no disease and pests harm；Seed group is placed in clean container and is stacked 12-18 hours, seed group is then put into clear water and is washed by rubbing with the hands, remove pulp and aril, reject impurity and empty grain, obtains complete full tsaoko seed；By tsaoko seed 5 DEG C, be protected from light under conditions of with sterile water impregnate 2-4 days；Processed tsaoko seed disinfection processing is placed at room ventilation, tsaoko the surface of the seed moisture is dried, tsaoko seed is being subjected to Stratificated treatment.The method provided by the invention for promoting tsaoko seed to sprout; germination rate has effectively facilitated the sprouting rate of tsaoko seed 80% or more, is of great significance to the cultivation and popularization and application of tsaoko; it is easy for workers to operate, be of very high actual application value to tsaoko seed seedling-raising, large-scale planting.
Description
Technical field
The present invention relates to field of plant growing technology, specially a kind of method for promoting tsaoko seed to sprout.
Background technique
Tsaoko (Amomum tsaoko Crevost et Lemarie) is under the jurisdiction of Zingiber (Zingiberaceae) Amomum
(Amomum), the provinces and regions such as Yunnan, Guangxi, Guizhou are originated in.Be used as medicine with dry mature fruit, have eliminating dampness middle benefit gas, preventing malaria eliminating phlegm it
Effect is used for internal retention of cold wetness, abdominal distention, feeling of fullness vomiting, malaria fever and chills and pestilence fever.In addition, tsaoko can also make flavoring,
Complete stool can extract aromatic oil.In production, tsaoko is unable to dry storage after tsaoko seed collection based on seminal propagation, shines
It is easy to lose vigor after dry, and the tsaoko seed of freshly harvested is in a dormant state, directly slowly, germination rate is low, out for sowing germination
Seedling is irregular.Existing research shows that the processing such as GA3, H2SO4, NaOH or wet sand hiding can promote the sprouting of tsaoko seed, but imitate
Fruit is bad, highest germination rate only 50% or so.
Summary of the invention
The present invention provides a kind of method that promotion tsaoko seed is sprouted, and can effectively solve mentioned above in the background art
Problem.
To achieve the above object, the invention provides the following technical scheme: it is a kind of promote tsaoko seed sprout method, including
Following steps:
S1, the tsaoko fruit for acquiring fresh mature no disease and pests harm, are placed in ventilative Polywoven Bag for the tsaoko fruit
Middle room temperature is stacked 2-3 days, is stripped outside rind, is obtained seed group；
S2, the seed group is placed in clean container and is stacked 12-18 hours, seed group is then put into clear water and is rubbed with the hands
It washes, removes pulp and aril, reject impurity and empty grain, obtain complete full tsaoko seed；
S3, by tsaoko seed 5 DEG C, be protected from light under conditions of with sterile water impregnate 2-4 days；
S4,30s, aseptic water washing will be impregnated for 75% ethyl alcohol through the processed tsaoko seed volumetric concentration of step S3
3 times, the NaClO solution disinfection 8min for being 10% with volumetric concentration, then with aseptic water washing 5-6 times, it is subsequently placed in room ventilation
Place, dries tsaoko the surface of the seed moisture；
S5, tsaoko seed is being subjected to Stratificated treatment, is by volume being 1: 4 sufficiently mixed with wet perlite when Stratificated treatment
Close, sealing be placed at 12-18 DEG C lamination 60-85 days, the wet perlite by 1 parts by weight perlite and 1.4-2 parts by weight
Water be uniformly mixed obtain.
S6, the tsaoko seed after Stratificated treatment is sown into and is sprouted in matrix, under two conditions alternate culture 28 days, first
Kind condition is that 12h is handled under 30 DEG C of illumination, and second of condition is to handle 12h under 20 DEG C of dark, and the sprouting matrix is by 1 parts by weight
Perlite and 2.5-3 parts by weight water be uniformly mixed be made.
Further, the perlite and water are by 121 DEG C, the processing of 20min high-temperature sterilization.
Further, it waters 3 times into polybag daily in tsaoko fruit stacking process.
Further, it breathes freely 1 time in the Stratificated treatment every 7 days depressurizations and adds 0.05-0.5 parts by weight water.
Compared with prior art, beneficial effects of the present invention:
(1), tsaoko seed is mixed into wet perlite and carries out Stratificated treatment, because of the water conservation of perlite, good permeability, can prevented
Only seed rots in lamination process, releases the suspend mode of seed, and the vigor of tsaoko seed has been effectively ensured, and promotes seed
Sprouting.
(2), using the light dark alternating vernalization that relents, the vigor of enzyme in tsaoko seed can be effectively improved, nutrition is promoted
Substance conversion, improves germination percentage.
(3), after stripping tsaoko fruit outside rind, seed group is placed in clean container and is stacked 12-18 hours, stacked
In the process due to the effect of enzyme, bacterium and temperature etc., seed rolls into a ball pulp softening, seed group is put into clear water again after stacking and washes by rubbing with the hands
Outer layer aril and pulp can be removed, washed by rubbing with the hands with existing using sand and plant ash, the seed impurity that this method obtains is few, does
Only, it chooses seeds convenient for the later period.
(4), tsaoko seed is carried out disinfection, perlite and water carry out high-temperature sterilization processing, can effectively reduce seed in layer
Mildew in long-pending or pregermination procedure.
(5), the method provided by the invention for promoting tsaoko seed to sprout, germination rate have effectively facilitated grass 80% or more
The sprouting rate of fruit seed is of great significance to the cultivation and popularization and application of tsaoko, and easy to operate, at low cost, is easy to people
Work operation, is of very high actual application value to tsaoko seed seedling-raising, large-scale planting.
Specific embodiment
Hereinafter, preferred embodiments of the present invention will be described, it should be understood that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1
A method of promote tsaoko seed to sprout, comprising the following steps:
S1, the tsaoko fruit for acquiring fresh mature no disease and pests harm, are placed in ventilative Polywoven Bag for the tsaoko fruit
Middle room temperature is stacked 2 days, is stripped outside rind, is obtained seed group；
S2, the seed group is placed in clean container and is stacked 12 hours, seed group is then put into clear water and is washed by rubbing with the hands, is gone
Except pulp and aril, impurity and empty grain are rejected, obtains complete full tsaoko seed；
S3, by tsaoko seed 5 DEG C, be protected from light under conditions of with sterile water impregnate 2 days；
S4,30s, aseptic water washing will be impregnated for 75% ethyl alcohol through the processed tsaoko seed volumetric concentration of step S3
3 times, the NaClO solution disinfection 8min for being 10% with volumetric concentration, then with aseptic water washing 5 times, be subsequently placed at room ventilation,
Dry tsaoko the surface of the seed moisture；
S5, tsaoko seed is being subjected to Stratificated treatment, is by volume being 1: 4 sufficiently mixed with wet perlite when Stratificated treatment
It closes, sealing is placed on lamination 60 days at 12 DEG C, and the wet perlite is mixed by the perlite of 1 parts by weight and the water of 1.4 parts by weight
Uniformly obtain.
S6, the tsaoko seed after Stratificated treatment is sown into and is sprouted in matrix, under two conditions alternate culture 28 days, first
Kind condition is that 12h is handled under 30 DEG C of illumination, and second of condition is to handle 12h under 20 DEG C of dark, and the sprouting matrix is by 1 parts by weight
Perlite and 2.5 parts by weight water be uniformly mixed be made.
In the present embodiment, the perlite and water are by 121 DEG C, the processing of 20min high-temperature sterilization.
In the present embodiment, water 3 times into polybag daily in tsaoko fruit stacking process.
In the present embodiment, breathes freely 1 time in the Stratificated treatment every 7 days depressurizations and add 0.05 parts by weight water.
Embodiment 2
A method of promote tsaoko seed to sprout, comprising the following steps:
S1, the tsaoko fruit for acquiring fresh mature no disease and pests harm, are placed in ventilative Polywoven Bag for the tsaoko fruit
Middle room temperature is stacked 2.5 days, is stripped outside rind, is obtained seed group；
S2, the seed group is placed in clean container and is stacked 15 hours, seed group is then put into clear water and is washed by rubbing with the hands, is gone
Except pulp and aril, impurity and empty grain are rejected, obtains complete full tsaoko seed；
S3, by tsaoko seed 5 DEG C, be protected from light under conditions of with sterile water impregnate 3 days；
S4,30s, aseptic water washing will be impregnated for 75% ethyl alcohol through the processed tsaoko seed volumetric concentration of step S3
3 times, the NaClO solution disinfection 8min for being 10% with volumetric concentration, then with aseptic water washing 5 times, be subsequently placed at room ventilation,
Dry tsaoko the surface of the seed moisture；
S5, tsaoko seed is being subjected to Stratificated treatment, is by volume being 1: 4 sufficiently mixed with wet perlite when Stratificated treatment
It closes, sealing is placed on lamination 73 days at 15 DEG C, and the wet perlite is mixed by the perlite of 1 parts by weight and the water of 1.7 parts by weight
Uniformly obtain.
S6, the tsaoko seed after Stratificated treatment is sown into and is sprouted in matrix, under two conditions alternate culture 28 days, first
Kind condition is that 12h is handled under 30 DEG C of illumination, and second of condition is to handle 12h under 20 DEG C of dark, and the sprouting matrix is by 1 parts by weight
Perlite and 2.8 parts by weight water be uniformly mixed be made.
In the present embodiment, the perlite and water are by 121 DEG C, the processing of 20min high-temperature sterilization.
In the present embodiment, water 3 times into polybag daily in tsaoko fruit stacking process.
In the present embodiment, breathes freely 1 time in the Stratificated treatment every 7 days depressurizations and add 0.2 parts by weight water.
Embodiment 3
A method of promote tsaoko seed to sprout, comprising the following steps:
S1, the tsaoko fruit for acquiring fresh mature no disease and pests harm, are placed in ventilative Polywoven Bag for the tsaoko fruit
Middle room temperature is stacked 3 days, is stripped outside rind, is obtained seed group；
S2, the seed group is placed in clean container and is stacked 18 hours, seed group is then put into clear water and is washed by rubbing with the hands, is gone
Except pulp and aril, impurity and empty grain are rejected, obtains complete full tsaoko seed；
S3, by tsaoko seed 5 DEG C, be protected from light under conditions of with sterile water impregnate 4 days；
S4,30s, aseptic water washing will be impregnated for 75% ethyl alcohol through the processed tsaoko seed volumetric concentration of step S3
3 times, the NaClO solution disinfection 8min for being 10% with volumetric concentration, then with aseptic water washing 6 times, be subsequently placed at room ventilation,
Dry tsaoko the surface of the seed moisture；
S5, tsaoko seed is being subjected to Stratificated treatment, is by volume being 1: 4 sufficiently mixed with wet perlite when Stratificated treatment
It closes, sealing is placed on lamination 85 days at 12-18 DEG C, and the wet perlite is mixed by the perlite of 1 parts by weight and the water of 2 parts by weight
Uniformly obtain.
S6, the tsaoko seed after Stratificated treatment is sown into and is sprouted in matrix, under two conditions alternate culture 28 days, first
Kind condition is that 12h is handled under 30 DEG C of illumination, and second of condition is to handle 12h under 20 DEG C of dark, and the sprouting matrix is by 1 parts by weight
Perlite and 3 parts by weight water be uniformly mixed be made.
In the present embodiment, the perlite and water are by 121 DEG C, the processing of 20min high-temperature sterilization.
In the present embodiment, water 3 times into polybag daily in tsaoko fruit stacking process.
In the present embodiment, breathes freely 1 time in the Stratificated treatment every 7 days depressurizations and add 0.5 parts by weight water.
The method provided by the invention for promoting tsaoko seed to sprout, germination rate have effectively facilitated tsaoko kind 80% or more
The sprouting rate of son, is of great significance to the cultivation and popularization and application of tsaoko, and easy to operate, at low cost, is easy for workers to grasp
Make, is of very high actual application value to tsaoko seed seedling-raising, large-scale planting.
Finally, it should be noted that being not intended to restrict the invention the foregoing is merely preferred embodiment of the invention, to the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, for those skilled in the art, still can be with
It modifies the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in guarantor of the invention
Within the scope of shield.
Claims (4)
1. a kind of method for promoting tsaoko seed to sprout, which comprises the following steps:
S1, the tsaoko fruit for acquiring fresh mature no disease and pests harm, are placed in room in ventilative Polywoven Bag for the tsaoko fruit
Temperature is stacked 2-3 days, is stripped outside rind, is obtained seed group；
S2, the seed group is placed in clean container and is stacked 12-18 hours, seed group is then put into clear water and is washed by rubbing with the hands, is gone
Except pulp and aril, impurity and empty grain are rejected, obtains complete full tsaoko seed；
S3, by tsaoko seed 5 DEG C, be protected from light under conditions of with sterile water impregnate 2-4 days；
S4, by through the processed tsaoko seed volumetric concentration of step S3 be 75% ethyl alcohol impregnate 30s, aseptic water washing 3 times,
The NaClO solution disinfection 8min for being 10% with volumetric concentration, then with aseptic water washing 5-6 times, be subsequently placed at room ventilation, dry in the air
Hay fruit the surface of the seed moisture；
S5, tsaoko seed is being subjected to Stratificated treatment, is by volume being to be sufficiently mixed at 1: 4 with wet perlite when Stratificated treatment, it is close
It is honored as a queen and is placed at 12-18 DEG C lamination 60-85 days, the wet perlite is mixed by the perlite of 1 parts by weight and the water of 1.4-2 parts by weight
Conjunction uniformly obtains,
S6, the tsaoko seed after Stratificated treatment is sown into and is sprouted in matrix, under two conditions alternate culture 28 days, the first
Part is that 12h is handled under 30 DEG C of illumination, and second of condition is that 12h is handled under 20 DEG C of dark, and the matrix of sprouting is by the treasure of 1 parts by weight
The water of Zhu Yan and 2.5-3 parts by weight, which is uniformly mixed, to be made.
2. a kind of method for promoting tsaoko seed to sprout according to claim 1, which is characterized in that the perlite and water
By 121 DEG C, the processing of 20min high-temperature sterilization.
3. a kind of method for promoting tsaoko seed to sprout according to claim 1, which is characterized in that stacked in tsaoko fruit
It waters 3 times into polybag daily in the process.
4. a kind of method for promoting tsaoko seed to sprout according to claim 1, which is characterized in that in the Stratificated treatment
It breathes freely 1 time every 7 days depressurizations and adds 0.05-0.5 parts by weight water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810413423.4A CN109923971A (en) | 2018-05-03 | 2018-05-03 | A method of promote tsaoko seed to sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810413423.4A CN109923971A (en) | 2018-05-03 | 2018-05-03 | A method of promote tsaoko seed to sprout |
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| Publication Number | Publication Date |
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| CN109923971A true CN109923971A (en) | 2019-06-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810413423.4A Withdrawn CN109923971A (en) | 2018-05-03 | 2018-05-03 | A method of promote tsaoko seed to sprout |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998183A (en) * | 2021-04-07 | 2021-06-22 | 西南大学 | Fresh-keeping storage method of fresh tsaoko fruits |
| CN114258753A (en) * | 2021-12-17 | 2022-04-01 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting germination of elaeagnus pungens seeds |
-
2018
- 2018-05-03 CN CN201810413423.4A patent/CN109923971A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998183A (en) * | 2021-04-07 | 2021-06-22 | 西南大学 | Fresh-keeping storage method of fresh tsaoko fruits |
| CN114258753A (en) * | 2021-12-17 | 2022-04-01 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting germination of elaeagnus pungens seeds |
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Application publication date: 20190625 |
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