CN109890985A - 检测寄生虫感染的方法和试剂盒 - Google Patents
检测寄生虫感染的方法和试剂盒 Download PDFInfo
- Publication number
- CN109890985A CN109890985A CN201880004078.7A CN201880004078A CN109890985A CN 109890985 A CN109890985 A CN 109890985A CN 201880004078 A CN201880004078 A CN 201880004078A CN 109890985 A CN109890985 A CN 109890985A
- Authority
- CN
- China
- Prior art keywords
- dna
- sample
- sequence
- group
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 100
- 208000030852 Parasitic disease Diseases 0.000 title claims abstract description 60
- 244000045947 parasite Species 0.000 claims abstract description 57
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 8
- 230000001225 therapeutic effect Effects 0.000 claims abstract description 8
- 108020004414 DNA Proteins 0.000 claims description 166
- 239000000523 sample Substances 0.000 claims description 157
- 208000009366 Echinococcosis Diseases 0.000 claims description 69
- 206010014096 Echinococciasis Diseases 0.000 claims description 61
- 241000244160 Echinococcus Species 0.000 claims description 54
- 230000003321 amplification Effects 0.000 claims description 35
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 35
- 238000012163 sequencing technique Methods 0.000 claims description 23
- 210000002381 plasma Anatomy 0.000 claims description 22
- 108010034145 Helminth Proteins Proteins 0.000 claims description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims description 19
- 244000000013 helminth Species 0.000 claims description 19
- 201000003808 Cystic echinococcosis Diseases 0.000 claims description 16
- 230000035772 mutation Effects 0.000 claims description 16
- 238000000265 homogenisation Methods 0.000 claims description 14
- 210000004369 blood Anatomy 0.000 claims description 13
- 239000008280 blood Substances 0.000 claims description 13
- 230000003252 repetitive effect Effects 0.000 claims description 13
- 244000078703 ectoparasite Species 0.000 claims description 12
- 238000004458 analytical method Methods 0.000 claims description 10
- 230000003071 parasitic effect Effects 0.000 claims description 10
- 241000242722 Cestoda Species 0.000 claims description 9
- 238000003745 diagnosis Methods 0.000 claims description 9
- 241000244170 Echinococcus granulosus Species 0.000 claims description 8
- 241000244163 Echinococcus multilocularis Species 0.000 claims description 8
- 239000012634 fragment Substances 0.000 claims description 8
- 210000002700 urine Anatomy 0.000 claims description 8
- 238000009396 hybridization Methods 0.000 claims description 7
- 210000003296 saliva Anatomy 0.000 claims description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
- 238000005516 engineering process Methods 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 claims description 6
- 108091093088 Amplicon Proteins 0.000 claims description 5
- 238000007403 mPCR Methods 0.000 claims description 5
- 241000244206 Nematoda Species 0.000 claims description 4
- 241001442514 Schistosomatidae Species 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 241001515965 unidentified phage Species 0.000 claims description 4
- 241000224489 Amoeba Species 0.000 claims description 3
- 241001465677 Ancylostomatoidea Species 0.000 claims description 3
- 241000244203 Caenorhabditis elegans Species 0.000 claims description 3
- 241000498255 Enterobius vermicularis Species 0.000 claims description 3
- 241000242711 Fasciola hepatica Species 0.000 claims description 3
- 239000012807 PCR reagent Substances 0.000 claims description 3
- 241000223996 Toxoplasma Species 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 206010014881 enterobiasis Diseases 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000011901 isothermal amplification Methods 0.000 claims description 2
- 238000002864 sequence alignment Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000007790 solid phase Substances 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 14
- 239000000047 product Substances 0.000 description 14
- 208000015181 infectious disease Diseases 0.000 description 13
- 238000013480 data collection Methods 0.000 description 12
- 239000011324 bead Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- 241000701959 Escherichia virus Lambda Species 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 238000012408 PCR amplification Methods 0.000 description 7
- 210000001124 body fluid Anatomy 0.000 description 7
- 239000010839 body fluid Substances 0.000 description 7
- 210000002726 cyst fluid Anatomy 0.000 description 7
- 235000019441 ethanol Nutrition 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 241000124008 Mammalia Species 0.000 description 6
- 239000013068 control sample Substances 0.000 description 6
- 229960004756 ethanol Drugs 0.000 description 6
- 150000007523 nucleic acids Chemical group 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 5
- 230000000875 corresponding effect Effects 0.000 description 5
- 206010019659 Hepatic echinococciasis Diseases 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 201000003894 alveolar echinococcosis Diseases 0.000 description 4
- 238000001574 biopsy Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 238000007405 data analysis Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 210000005259 peripheral blood Anatomy 0.000 description 4
- 239000011886 peripheral blood Substances 0.000 description 4
- 229920001223 polyethylene glycol Polymers 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 108091033319 polynucleotide Proteins 0.000 description 3
- 102000040430 polynucleotide Human genes 0.000 description 3
- 239000002157 polynucleotide Substances 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000208340 Araliaceae Species 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 241001269238 Data Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000009514 concussion Effects 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 235000008434 ginseng Nutrition 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000222722 Leishmania <genus> Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 241000224016 Plasmodium Species 0.000 description 1
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000012642 Sexual Trauma Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000010828 animal waste Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- -1 chip) hybrid capture Substances 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 208000019503 echinococcus granulosus infectious disease Diseases 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 231100000255 pathogenic effect Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明提供一种检测样品中寄生虫来源的DNA的方法，以及利用该检测方法诊断宿主对象是否正经受寄生虫感染的方法，确定寄生虫感染治疗功效的方法，以及筛选寄生虫感染候选治疗的方法，还提供用于上述方法的试剂盒。
Description
PCT国内申请，说明书已公开。
Claims (30)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710554315 | 2017-07-02 | ||
| CN2017105543154 | 2017-07-02 | ||
| PCT/CN2018/093254 WO2019007251A1 (zh) | 2017-07-02 | 2018-06-28 | 检测寄生虫感染的方法和试剂盒 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109890985A true CN109890985A (zh) | 2019-06-14 |
Family
ID=64949710
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880004078.7A Pending CN109890985A (zh) | 2017-07-02 | 2018-06-28 | 检测寄生虫感染的方法和试剂盒 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US11702705B2 (zh) |
| CN (1) | CN109890985A (zh) |
| WO (1) | WO2019007251A1 (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518877A (zh) * | 2020-05-12 | 2020-08-11 | 青海大学 | 可用于微量样本分型检测多房棘球蚴和细粒棘球蚴的一管法巢式实时定量pcr检测试剂盒 |
| CN112501280A (zh) * | 2020-01-22 | 2021-03-16 | 微岩医学科技(北京)有限公司 | 一种基于高通量测序的泡型包虫高灵敏度检测方法 |
| CN113355431A (zh) * | 2020-08-24 | 2021-09-07 | 四川大学华西医院 | 肝包虫基因片段筛选方法、扩增引物及试剂盒 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154905A (zh) * | 2019-06-11 | 2020-05-15 | 青海大学附属医院 | 检测泡型包虫感染的方法和试剂盒 |
| CN111381021A (zh) * | 2020-03-04 | 2020-07-07 | 南京亿科人群健康研究院有限公司 | 一种基于检测包虫特异性抗原及其编码基因的早期诊断方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103374615A (zh) * | 2012-04-20 | 2013-10-30 | 广州世优生物科技有限公司 | 犬囊型包虫病pcr检测试剂盒 |
| CN104619858A (zh) * | 2012-07-31 | 2015-05-13 | 深圳华大基因科技有限公司 | 胎儿健康状态的无创性检测 |
| CN105018613A (zh) * | 2015-07-17 | 2015-11-04 | 上海师范大学 | 一种从狐狸粪中检测多房棘球绦虫的方法 |
-
2018
- 2018-06-28 CN CN201880004078.7A patent/CN109890985A/zh active Pending
- 2018-06-28 US US16/628,259 patent/US11702705B2/en active Active
- 2018-06-28 WO PCT/CN2018/093254 patent/WO2019007251A1/zh active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103374615A (zh) * | 2012-04-20 | 2013-10-30 | 广州世优生物科技有限公司 | 犬囊型包虫病pcr检测试剂盒 |
| CN104619858A (zh) * | 2012-07-31 | 2015-05-13 | 深圳华大基因科技有限公司 | 胎儿健康状态的无创性检测 |
| CN105018613A (zh) * | 2015-07-17 | 2015-11-04 | 上海师范大学 | 一种从狐狸粪中检测多房棘球绦虫的方法 |
Non-Patent Citations (2)
| Title |
|---|
| WANG等: ""Diagnosis of Pneumocystis jirovecii pneumonia with serum cell-free DNA in non-HIV-infected immunocompromised patients"", 《ONCOTARGET》 * |
| WICHMANN等: ""Diagnosing Schistosomiasis by Detection of Cell -Free Parasite DNA in Human Plasma"", 《PLOS NEGLECTED TROPICAL DISEASES》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112501280A (zh) * | 2020-01-22 | 2021-03-16 | 微岩医学科技(北京)有限公司 | 一种基于高通量测序的泡型包虫高灵敏度检测方法 |
| CN111518877A (zh) * | 2020-05-12 | 2020-08-11 | 青海大学 | 可用于微量样本分型检测多房棘球蚴和细粒棘球蚴的一管法巢式实时定量pcr检测试剂盒 |
| CN113355431A (zh) * | 2020-08-24 | 2021-09-07 | 四川大学华西医院 | 肝包虫基因片段筛选方法、扩增引物及试剂盒 |
Also Published As
| Publication number | Publication date |
|---|---|
| US11702705B2 (en) | 2023-07-18 |
| US20200216915A1 (en) | 2020-07-09 |
| WO2019007251A1 (zh) | 2019-01-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109890985A (zh) | 检测寄生虫感染的方法和试剂盒 | |
| CN103298955A (zh) | 用于构建血浆dna测序文库的方法和试剂盒 | |
| CN106755464A (zh) | 用于筛选肠癌和/或胃癌的基因标志物的方法、用该方法筛选的基因标志物及其用途 | |
| CN110029158A (zh) | 一种马凡综合征检测panel及其应用 | |
| CN111778332A (zh) | 一种用于腺瘤及结直肠癌早期诊断的标志物组合及试剂盒 | |
| CN106755350A (zh) | cfDNA文库qPCR定量标准品的制备方法 | |
| CN112852957B (zh) | 先天性巨结肠早期诊断标志物及其应用 | |
| CN102732633B (zh) | 人idh基因突变的检测引物和试剂盒 | |
| US20210317516A1 (en) | Method for constructing library of cell-free dnas in body fluids and application thereof | |
| CN108060227A (zh) | 一种检测pah基因突变的扩增引物、试剂盒及其检测方法 | |
| CN113999901B (zh) | 心肌特异性甲基化标记物 | |
| CN108192965A (zh) | 一种检测线粒体基因组a3243g位点异质性的方法 | |
| CN108048589A (zh) | 牛双芽巴贝斯虫的Real-time PCR特异性引物和探针及检测试剂盒与检测方法 | |
| CN107164508A (zh) | 用于检测肝癌的基因标志物及其用途 | |
| US20240200151A1 (en) | Metagenomic next-generation sequencing of microbial cell-free nucleic acids in subjects with lyme disease | |
| CN108103196A (zh) | 一种用于检测食管癌的基因标志物及其用途和检测方法 | |
| CA3232274A1 (en) | Drain fluid for diagnostics | |
| CN111690736A (zh) | 一种华法林用药基因检测试剂盒及使用方法 | |
| CN105316350B (zh) | 结核分枝杆菌EmbB突变基因及其用途 | |
| RU2762759C1 (ru) | Способ пробоподготовки образцов изолятов коронавируса SARS-CoV-2 и олигонуклеотидные праймеры для его реализации | |
| CN107419009A (zh) | 一种检测胃肠道间质瘤相关基因突变的试剂盒及其应用 | |
| US11718880B2 (en) | Marker and diagnosis method for noninvasive diagnosis of myocardial infarction | |
| Callado Prat | Comparison of manual and semi-automatic RNA extraction methods using two-tailed RT-qPCR for absolute quantification as part of the sepsis diagnosis research | |
| CN114395623B (zh) | 一种基因甲基化检测引物组合物及其试剂盒和应用 | |
| CN109022566B (zh) | 发作性运动诱发性运动障碍相关的基因突变靶点及其应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190614 |