CN109890985A - 检测寄生虫感染的方法和试剂盒 - Google Patents

检测寄生虫感染的方法和试剂盒 Download PDF

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Publication number
CN109890985A
CN109890985A CN201880004078.7A CN201880004078A CN109890985A CN 109890985 A CN109890985 A CN 109890985A CN 201880004078 A CN201880004078 A CN 201880004078A CN 109890985 A CN109890985 A CN 109890985A
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dna
sample
sequence
group
kit
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张灼华
万正卿
凌捷
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Xiangya Hospital of Central South University
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Xiangya Hospital of Central South University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明提供一种检测样品中寄生虫来源的DNA的方法,以及利用该检测方法诊断宿主对象是否正经受寄生虫感染的方法,确定寄生虫感染治疗功效的方法,以及筛选寄生虫感染候选治疗的方法,还提供用于上述方法的试剂盒。

Description

PCT国内申请,说明书已公开。

Claims (30)

  1. PCT国内申请,权利要求书已公开。
CN201880004078.7A 2017-07-02 2018-06-28 检测寄生虫感染的方法和试剂盒 Pending CN109890985A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201710554315 2017-07-02
CN2017105543154 2017-07-02
PCT/CN2018/093254 WO2019007251A1 (zh) 2017-07-02 2018-06-28 检测寄生虫感染的方法和试剂盒

Publications (1)

Publication Number Publication Date
CN109890985A true CN109890985A (zh) 2019-06-14

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CN201880004078.7A Pending CN109890985A (zh) 2017-07-02 2018-06-28 检测寄生虫感染的方法和试剂盒

Country Status (3)

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US (1) US11702705B2 (zh)
CN (1) CN109890985A (zh)
WO (1) WO2019007251A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111518877A (zh) * 2020-05-12 2020-08-11 青海大学 可用于微量样本分型检测多房棘球蚴和细粒棘球蚴的一管法巢式实时定量pcr检测试剂盒
CN112501280A (zh) * 2020-01-22 2021-03-16 微岩医学科技(北京)有限公司 一种基于高通量测序的泡型包虫高灵敏度检测方法
CN113355431A (zh) * 2020-08-24 2021-09-07 四川大学华西医院 肝包虫基因片段筛选方法、扩增引物及试剂盒

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111154905A (zh) * 2019-06-11 2020-05-15 青海大学附属医院 检测泡型包虫感染的方法和试剂盒
CN111381021A (zh) * 2020-03-04 2020-07-07 南京亿科人群健康研究院有限公司 一种基于检测包虫特异性抗原及其编码基因的早期诊断方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103374615A (zh) * 2012-04-20 2013-10-30 广州世优生物科技有限公司 犬囊型包虫病pcr检测试剂盒
CN104619858A (zh) * 2012-07-31 2015-05-13 深圳华大基因科技有限公司 胎儿健康状态的无创性检测
CN105018613A (zh) * 2015-07-17 2015-11-04 上海师范大学 一种从狐狸粪中检测多房棘球绦虫的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103374615A (zh) * 2012-04-20 2013-10-30 广州世优生物科技有限公司 犬囊型包虫病pcr检测试剂盒
CN104619858A (zh) * 2012-07-31 2015-05-13 深圳华大基因科技有限公司 胎儿健康状态的无创性检测
CN105018613A (zh) * 2015-07-17 2015-11-04 上海师范大学 一种从狐狸粪中检测多房棘球绦虫的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WANG等: ""Diagnosis of Pneumocystis jirovecii pneumonia with serum cell-free DNA in non-HIV-infected immunocompromised patients"", 《ONCOTARGET》 *
WICHMANN等: ""Diagnosing Schistosomiasis by Detection of Cell -Free Parasite DNA in Human Plasma"", 《PLOS NEGLECTED TROPICAL DISEASES》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112501280A (zh) * 2020-01-22 2021-03-16 微岩医学科技(北京)有限公司 一种基于高通量测序的泡型包虫高灵敏度检测方法
CN111518877A (zh) * 2020-05-12 2020-08-11 青海大学 可用于微量样本分型检测多房棘球蚴和细粒棘球蚴的一管法巢式实时定量pcr检测试剂盒
CN113355431A (zh) * 2020-08-24 2021-09-07 四川大学华西医院 肝包虫基因片段筛选方法、扩增引物及试剂盒

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US11702705B2 (en) 2023-07-18
US20200216915A1 (en) 2020-07-09

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Application publication date: 20190614