CN109864911A - A kind of application for avenging tea extraction - Google Patents
A kind of application for avenging tea extraction Download PDFInfo
- Publication number
- CN109864911A CN109864911A CN201711249852.4A CN201711249852A CN109864911A CN 109864911 A CN109864911 A CN 109864911A CN 201711249852 A CN201711249852 A CN 201711249852A CN 109864911 A CN109864911 A CN 109864911A
- Authority
- CN
- China
- Prior art keywords
- tea extraction
- related gene
- gene
- aging
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 47
- 241001122767 Theaceae Species 0.000 title claims abstract 12
- 210000003491 skin Anatomy 0.000 claims abstract description 40
- 230000014509 gene expression Effects 0.000 claims abstract description 36
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 29
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 230000003712 anti-aging effect Effects 0.000 claims abstract description 15
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 14
- 210000002615 epidermis Anatomy 0.000 claims abstract description 13
- 239000000470 constituent Substances 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 13
- 239000000284 extract Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 235000019441 ethanol Nutrition 0.000 claims description 5
- 101150017002 CD44 gene Proteins 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 4
- 101150008656 COL1A1 gene Proteins 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 abstract description 9
- 108010029483 alpha 1 Chain Collagen Type I Proteins 0.000 abstract description 5
- 230000036548 skin texture Effects 0.000 abstract description 4
- 239000000419 plant extract Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 244000269722 Thea sinensis Species 0.000 description 42
- 235000013616 tea Nutrition 0.000 description 42
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 9
- 229920002674 hyaluronan Polymers 0.000 description 9
- 229960003160 hyaluronic acid Drugs 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 102100032912 CD44 antigen Human genes 0.000 description 7
- 102000008186 Collagen Human genes 0.000 description 7
- 108010035532 Collagen Proteins 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 229920001436 collagen Polymers 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- 230000006870 function Effects 0.000 description 6
- 102100036597 Basement membrane-specific heparan sulfate proteoglycan core protein Human genes 0.000 description 5
- 108010049224 perlecan Proteins 0.000 description 5
- 108010022452 Collagen Type I Proteins 0.000 description 4
- 102000012422 Collagen Type I Human genes 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 230000003110 anti-inflammatory effect Effects 0.000 description 3
- 210000002469 basement membrane Anatomy 0.000 description 3
- 238000010195 expression analysis Methods 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000009759 skin aging Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 108091016585 CD44 antigen Proteins 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 102000008055 Heparan Sulfate Proteoglycans Human genes 0.000 description 2
- 229920002971 Heparan sulfate Polymers 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108090000054 Syndecan-2 Proteins 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 210000002919 epithelial cell Anatomy 0.000 description 2
- 229960004756 ethanol Drugs 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000004927 skin cell Anatomy 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- CUCUKLJLRRAKFN-UHFFFAOYSA-N 7-Hydroxy-(S)-usnate Chemical compound CC12C(=O)C(C(=O)C)C(=O)C=C1OC1=C2C(O)=C(C)C(O)=C1C(C)=O CUCUKLJLRRAKFN-UHFFFAOYSA-N 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 102000002734 Collagen Type VI Human genes 0.000 description 1
- 108010043741 Collagen Type VI Proteins 0.000 description 1
- 102100033601 Collagen alpha-1(I) chain Human genes 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 240000003173 Drymaria cordata Species 0.000 description 1
- 206010013954 Dysphoria Diseases 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 206010019345 Heat stroke Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 108010067787 Proteoglycans Proteins 0.000 description 1
- 102000016611 Proteoglycans Human genes 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241001646982 Thamnolia vermicularis Species 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 102100023935 Transmembrane glycoprotein NMB Human genes 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- YTJJRAWFHJBAMT-UHFFFAOYSA-N depside Natural products OC(=O)CC1=C(O)C=C(O)C=C1OC(=O)C1=CC=C(O)C(O)=C1 YTJJRAWFHJBAMT-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000013538 functional additive Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000001365 lymphatic vessel Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- -1 phenolic aldehyde acids Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000000225 synapse Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 108091007466 transmembrane glycoproteins Proteins 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 229940004858 usnic acid Drugs 0.000 description 1
- ICTZCAHDGHPRQR-UHFFFAOYSA-N usnic acid Natural products OC1=C(C)C(O)=C(C(C)=O)C2=C1C1(C)C(O)=C(C(=O)C)C(=O)C=C1O2 ICTZCAHDGHPRQR-UHFFFAOYSA-N 0.000 description 1
- WEYVVCKOOFYHRW-UHFFFAOYSA-N usninic acid Natural products CC12C(=O)C(C(=O)C)=C(O)C=C1OC1=C2C(O)=C(C)C(O)=C1C(C)=O WEYVVCKOOFYHRW-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 235000020334 white tea Nutrition 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to daily use chemicals and plant extracts fields, and in particular to avenges the application of tea extraction.Avenging tea extraction has the ability for improving basilar memebrane related gene and anti-aging related gene expression, in content 0.001%, the expression that basilar memebrane stablizes related gene hspg2, senile-resistant efficacy related gene COL1A1 and moisturizing related gene CD44 can be effectively improved；Snow tea extraction can be used as moisturizing, anti-aging, firm skin texture or the active constituent for stablizing true epidermis connection, be used to prepare skin preparations for extenal use.
Description
Technical field
The present invention relates to cosmetics and plant extracts fields, and in particular to avenges the application of tea extraction.
Background technique
Skin covering is placed on entire human body surface, directly contacts with external environment, in addition to protecting body from extraneous various
It outside the injury of factor, and is the mirror for reacting general health status.Skin is made of epidermis, corium, subcutaneous tissue, and is contained
There are cutaneous appendages, nerve, blood vessel, lymphatic vessel and muscle abundant.Basilar memebrane is also known as in the join domain of corium and epidermis
Band is not only a model of a layered structure, and has various functions, and basilar memebrane is the supporting pad of epithelial cell, thin in epithelium
Structure connection function is played between born of the same parents' tissue and connective tissue, meanwhile, also there is otherwise effect.For example, basilar memebrane conduct
The selectivity of cell usually can prevent phoirocyte from contacting with the epithelial cell on basilar memebrane by barrier, but allow
Lymphocyte, macrophage and neuronal synapse pass through；In addition, the form of cell, the polarity of cell, cell metabolism, matter
Many biological phenomenas such as the distribution of protein, cell survival, the Proliferation, Differentiation of cell, cell migration, have with basilar memebrane on film
Unusual close relationship.
Heparan sulfate proteoglycan hspg2 (heparan sulfate proteoglycan) gene coded base film is poly-
Glycoprotein (perlecan) is a main component of basilar memebrane, by the core protein of the glycosaminoglycan connection of three long-chains
Composition.Perlecan albumen is the proteoglycans of a Multidomain, in conjunction with and be crosslinked many extracellular matrix components and thin
Cellular surface molecule.It is existing research it has been shown that the protein and laminin, degreasing protein, IV Collagen Type VI etc. interaction,
It plays an important role in multiple biological activities.Perlecan can also promote growth factor activity, thus stimulate endothelial growth and
Regeneration.The expression that can promote perlecan albumen is raised in hspg2 gene expression, and then promotes the firm of basement membrane structure
Property, strengthen the close connection of true epidermis.
Type i collagen is the main ingredient of dermal layer of the skin.Increase with the age, the collagen of skin corium can gradually aging,
It is lost, content is gradually reduced.This is a principal element for leading to skin aging.The loss of collagen, after directly resulting in
Fruit is exactly skin histology atrophy, collapses, and the aging phenomenons such as dry, coarse, loose, wrinkle occurs in skin.Skin is improved by stimulation
The ability of skin cell synthesis type i collagen is an effective means of anti-aging.1 chain of COL1A1 genes encoding Type I collagen α, therefore
Increase COL1A1 gene expression can promote I-type collagen in corium generation, synthesis and assembly extracellular matrix, enhancing with
Support the stability of skin texture, delay skin aging.
CD44 is cell surface trans-membrane glycoprotein, and other than it can promote epidermal layer cells cell differentiation and proliferation, it is also
Can be in conjunction with hyaluronic acid, thus the receptor of also referred to as hyaluronic acid.Hyaluronic acid is bundled in carefully by cell by CD44
Extracellular space, for maintaining intercellular high-moisture.In addition, CD44 also plays important work in the synthesis process of hyaluronic acid
With the expression quantity of CD44 determines the content of hyaluronic acid in skin in Skin Cell.But as the age increases, internal CD44 meeting
Gradually decrease, vigor also and then declines, the problem of this is also dry skin water shortage root.It, can by activating the expression of CD44
The self task performance for updating, manufacturing hyaluronic acid is promoted, the moisture-retaining capacity of skin is promoted.
Therefore, how research improves the expression of skin key position and the relevant gene of function, for improving the true table of skin
The stability of skin structure, delay skin aging are of great significance.
Bai Xuecha is the drying thallus that ground Tea plant avenges tea, scientific name Thamnolia vermicularis also known as ground
Tea, too white tea.Bai Xuecha is mainly distributed on 4000-5000 meters of the height above sea level such as Sichuan Province China province, Yunnan Province, Shaanxi Province and Qinghai-Tibet Platean
Plateau.The thallus of Bai Xuecha is in small thorn-like bifurcated, small-sized plexi growth, surface milky or canescence, matt, light
Sliding, free bend when dry is 3-4 millimeters thick, hollow, about 3-7 centimetres high, and upright or prostrate gathers the grass of the high mountain near snow line
Pasture, dark and damp rock, tongue fur and flowstone beach on, only have be best period of its growth procreation in 1 year within 2-3 months, and grow ten
Divide slow.
Early in the Ming Dynasty, avenges tea and have become the treasure that Lijing Mu Shi toast is paid tribute.It is natural as tradition that ethnic group will avenge tea
Health tea or conventional medicament have clearing heat and detoxicating, relieving cough and reducing sputum, nourishing heart improving eyesight, refreshment and tranquilization, anti-inflammatory and other effects using so far.
" national Chinese herbal medicine compilation ", which records snow tea, can treat heatstroke, in the heart dysphoria with smothery sensation, deficiency of Yin hectic fever, cough with lung heat, neurasthenia, high blood
Press disease etc..
Research in recent years from snow tea in it is isolated include depside, steroid, polysaccharide, fatty acid, carrotene,
Hundreds of compound [2,7,10] including the ingredients such as polyalcohol, free amino acid.Avenge in tea has high potassium low in macroelement
The characteristics of sodium, and it is rich in Ca and Fe element, there is good supplementary function to human body.Avenge a large amount of contracting phenolic aldehyde acids chemical combination in tea
Object --- snow theine has the physiological functions such as anti-inflammatory, antibacterial, antipyretic, adjusting abdominal pain.Usnic acid has antibacterial activity；Peroxidating
Ergosterol then has stronger anti-tumor activity and anti-inflammatory efficacy.
Summary of the invention
The present invention is intended to provide snow tea extraction answering in terms of preparing with function of delaying senility, moisturizing skin preparations for extenal use
With.
Snow tea extraction can consolidate skin texture, stablize true epidermis connection, have the effect of anti-aging.Therefore, tea is avenged
Extract is used to prepare skin preparations for extenal use, can be used as anti-aging, firm skin texture or the active constituent for stablizing true epidermis connection.
Avenge tea extraction as improve I-type collagen synthesis, improve hyaluronic acid contents or
Avenging tea extraction has the energy for improving basilar memebrane related gene, anti-aging related gene and moisturizing related gene expression
Power.
Preferably, the senile-resistant efficacy related gene is COL1A1 gene, and snow tea extraction can be improved COL1A1 base
Because of expression, promote the synthesis of collagen, keep the collagen of skin corium, to delay senescence.
Preferably, it is hspg2 gene that the basilar memebrane, which stablizes related gene, and snow tea extraction can be improved hspg2 gene
Expression promotes the expression of perlecan albumen, and then promotes the stability of basement membrane structure, strengthens the close connection of true epidermis.
Preferably, the moisturizing related gene is CD44 gene, and snow tea extraction can be improved the expression of CD44 gene,
To promote the synthesis of hyaluronic acid, increase the content of hyaluronic acid in skin, improves skin moisture-keeping ability.
Above-mentioned snow tea extraction is water extract, extractive with organic solvent, water and the organic solvent mixed liquor for avenging tea
Extract or above-mentioned any mixture.
The preparation method of snow tea extraction includes the group of decoction, ultrasonic extraction, extraction, reflux, diacolation or the above method
It closes.
Preferably, snow tea extraction can be solvent with the mixed liquor of water, alcohol or water and alcohol, and by decocting, ultrasound mentions
It takes, extract, flowing back or the method for diacolation extracts.
More preferably, avenge the preparation method of tea extraction the following steps are included:
(1) Bai Xuecha thallus is extracted 1~3 time with 60%~80% EtOH Sonicate, merging filtrate；
(2) solvent is removed.
It is 10~500 mesh powders that the Bai Xuecha thallus, which is water content lower than 5%, partial size,.
Above-mentioned snow tea extraction has the ability for consolidating true epidermis concatenation ability and anti-aging, can be used as effect addition
Agent is added in cosmetics and uses.It, can be effective when content is 10 μ g/mL (about 0.001wt%, in terms of the dry matter of extract)
Improve senile-resistant efficacy related gene COL1A1, basilar memebrane stablizes related gene hspg2 and moisturizing related gene is CD44's
Expression.
Avenging content of the tea extraction in skin preparations for extenal use is 0.0005wt%~5wt%, preferably 0.001wt%~
3wt%.The skin preparations for extenal use, such as cosmetics, type include that washing cream, lotion, creams, Essence, toner are (such as soft
Skin water, toner), facial mask, makeup remover, Gel miles, it is spraying etc..
New use the present invention provides snow tea extraction as skin preparations for extenal use (cosmetics, skin care item etc.) functional additive
On the way, it can be used as active constituent, be used to prepare to have and stablize basal membrane structure, stablize true epidermis connection or anti-aging, moisturizing
The skin preparations for extenal use of effect；Snow tea extraction preparation method is simple, and process conditions are easily-controllable；Extract activity obtained is high,
Remarkable result can be generated in the case that content is extremely low, it is natural, safe and effective, do not stimulate skin.
Detailed description of the invention
Fig. 1 is the expression avenged tea extraction and basilar memebrane is promoted to stablize related gene hspg2
Fig. 2 is the expression avenged tea extraction and promote senile-resistant efficacy related gene COL1A1
Fig. 3 is the expression avenged tea extraction and promote skin moisture-keeping effect related gene CD44
Specific embodiment
The production of the snow tea extraction of embodiment 1
(1) Bai Xuecha thallus is dry lower than 5% and is crushed to 200 mesh to water content, takes 10g that 70% ethyl alcohol (body is added
Product concentration), 500W ultrasonic extraction 2 times at room temperature extract 30min with 100mL ethyl alcohol every time；
(2) merging filtrate, rotary evaporated to dryness；Obtain the white snow tea extraction of about 0.8g.
Embodiment 2 avenges the expression that tea extraction promotes basilar memebrane to stablize related gene hspg2
(1) preparation of skin model
Extract people's primary fibroblast (NHDF) and people's primary keratinocyte (NHEK) respectively from skin histology.By people
Primary fibroblast recovery mixes with collagen after culture digestion and carries out corium stage culture 3~4 days, then will use raising
People's primary keratinocyte of layer culture, the skin model surface of digestive inoculation to corium stage, successively by epidermis submergence culture
And liquid phase cultivation stage, culture obtained the 3D skin model that can be used for detecting to 18 days.
In incubation, the D3 after being inoculated with fibroblast on bracket uses ordinary culture medium to final D18 respectively
(control group, NT) or contain 10 μ g/mL embodiments 1 snow tea extraction (about 0.001wt%, in terms of the dry weight for avenging tea extraction)
Culture medium skin model is cultivated.
(2) extraction of RNA
Clip tissue about 2*3mm is put into 2mL EP pipe；1mL Trizol is added, 2 steel balls are added, use historrhexis
Instrument homogenate, 50Hz, 5min；Supernatant is transferred in new 1.5mL EP pipe by 9000rpm, 4 DEG C of 1min；200ul chlorine is added
It is imitative, it gently overturns and mixes for several times, be placed at room temperature for 5 minutes；9000rpm, 4 DEG C of 15min；Turn upper strata aqueous phase (about 400uL) Yu Xin
In 1.5mL EP pipe, isometric isopropanol is added, mixes, is stored at room temperature 10min；9000rpm 10min 4℃；Supernatant is abandoned,
70% dehydrated alcohol of precipitating pre-cooling is washed 1 time, and 4 DEG C of 8000rpm 5min；Supernatant is abandoned, is air-dried 5-10 minutes, is dissolved in
In 30ul DEPC water；Spectrophotometric determination RNA concentration.
(3) gene expression analysis
The purifying and gene quantification analysis that RNA is carried out according to QuantiGeneR 2.0Plex Assay kit, use
Luminex platform analyzes gene expression.
As a result as shown in Figure 1.As shown in Figure 1, compared with control group (NT), after addition snow tea extraction, in skin model
In stablize relevant hspg2 gene expression to basilar memebrane and raise 1.6 times, illustrate the extract can be improved basilar memebrane stablize it is related
The expression of gene plays the role of promoting basement membrane structure stable.
In the test of measurement gene expression, if the expression quantity of gene is bright relative to the experimental group for being not added with extract
Aobvious to increase, deducibility snow tea extraction can induce basilar memebrane to stablize relevant gene, the relevant gene of moisturizing and anti-aging
Relevant gene expression has effects that the stabilization, moisturizing and the anti-aging that improve the structure of skin.
The relevant gene expression of 3 anti-aging of embodiment
3D cell model cultural method, RNA tissue extraction method and gene expression analysis method are the same as embodiment 2.With right
It is compared according to group (NT), in the skin model of addition 10 μ g/mL snow tea extraction, gene C OL1A1 relevant to type i collagen synthesis
The obvious up-regulation (1.42 times) of expression, as a result as shown in Figure 2.Illustrate that avenging tea extraction can be improved the expression of anti-aging related gene,
With senile-resistant efficacy.
The relevant gene expression of 4 moisturizing of embodiment
3D cell model cultural method, RNA tissue extraction method and gene expression analysis method are the same as embodiment 2.With right
It is compared according to group (NT), in the skin model of addition 10 μ g/mL snow tea extraction, in CD44 gene expression relevant to skin moisture-keeping
1.51 times are adjusted, as a result as shown in Figure 3.Illustrate that avenging tea extraction can be improved the expression of anti-moisturizing related gene, the function with moisturizing
Effect.
Claims (10)
1. avenging application of the tea extraction in terms of preparing skin preparations for extenal use, which is characterized in that the snow tea extraction is used as steady
The active constituent determined basal membrane structure, stablize true epidermis connection, anti-aging or moisturizing.
2. application according to claim 1, which is characterized in that the snow tea extraction, which is used as, improves basilar memebrane stable phase
Correlation gene expression, and/or raising anti-aging related gene expression, and/or raising moisturizing related gene expression active constituent.
3. application according to claim 2, which is characterized in that it is hspg2 gene that the basilar memebrane, which stablizes related gene,；
The senile-resistant efficacy related gene is COL1A1 gene；The moisturizing related gene is CD44 gene.
4. described in any item applications according to claim 1~3, which is characterized in that the snow tea extraction is Bai Xuecha's
Extract or the above-mentioned any mixture of water extract, extractive with organic solvent, water and organic solvent mixed liquor.
5. described in any item applications according to claim 1~3, which is characterized in that the preparation method of the snow tea extraction
The following steps are included:
(1) Bai Xuecha thallus is extracted 1~3 time with 60%~80% EtOH Sonicate, merging filtrate；
(2) solvent is removed.
6. application described in claim 5, which is characterized in that in step (1), when each ultrasonic extraction, 60%~80% ethyl alcohol
Amount ratio with Bai Xuecha thallus is 5~20L:1kg.
7. application described in claim 5, which is characterized in that the Bai Xuecha thallus is water content is lower than 5%, partial size
10~500 mesh powders.
8. it is a kind of with the skin preparations for extenal use stablized basal membrane structure, stablize the connection of true epidermis, anti-aging or moisture-keeping function,
It is characterized in that, containing snowy tea extraction.
9. skin preparations for extenal use according to any one of claims 8, which is characterized in that the content for avenging tea extraction is 0.0005%~5%.
10. skin preparations for extenal use according to any one of claims 8, which is characterized in that the content for avenging tea extraction is 0.001%~3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711249852.4A CN109864911A (en) | 2017-12-01 | 2017-12-01 | A kind of application for avenging tea extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711249852.4A CN109864911A (en) | 2017-12-01 | 2017-12-01 | A kind of application for avenging tea extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109864911A true CN109864911A (en) | 2019-06-11 |
Family
ID=66914583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711249852.4A Pending CN109864911A (en) | 2017-12-01 | 2017-12-01 | A kind of application for avenging tea extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109864911A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484583A (en) * | 2019-07-10 | 2019-11-22 | 广西慧宝源医药科技有限公司 | Bird's nest active peptide and its antitumor application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006056854A (en) * | 2004-08-23 | 2006-03-02 | Maruzen Pharmaceut Co Ltd | Immunostimulating agent, antineoplastic agent, antiinflammatory agent, antiaging agent and skin cosmetic |
| CN102552337A (en) * | 2012-02-21 | 2012-07-11 | 中国人民解放军第三0二医院 | Extract of lethariella cladonioides active site for reducing fat and protecting liver and application for extract |
| CN103260618A (en) * | 2010-12-13 | 2013-08-21 | 花王株式会社 | Inhibitor of activity of endothelin, and skin whitening agent |
| CN106074263A (en) * | 2016-07-05 | 2016-11-09 | 上海相宜本草化妆品股份有限公司 | A kind of book on Chinese herbal medicine extract and the application in cosmetics thereof |
-
2017
- 2017-12-01 CN CN201711249852.4A patent/CN109864911A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006056854A (en) * | 2004-08-23 | 2006-03-02 | Maruzen Pharmaceut Co Ltd | Immunostimulating agent, antineoplastic agent, antiinflammatory agent, antiaging agent and skin cosmetic |
| CN103260618A (en) * | 2010-12-13 | 2013-08-21 | 花王株式会社 | Inhibitor of activity of endothelin, and skin whitening agent |
| CN102552337A (en) * | 2012-02-21 | 2012-07-11 | 中国人民解放军第三0二医院 | Extract of lethariella cladonioides active site for reducing fat and protecting liver and application for extract |
| CN106074263A (en) * | 2016-07-05 | 2016-11-09 | 上海相宜本草化妆品股份有限公司 | A kind of book on Chinese herbal medicine extract and the application in cosmetics thereof |
Non-Patent Citations (3)
| Title |
|---|
| 程挚: ""云南白雪茶研究现状及开发利用"", 《安徽农业科学》 * |
| 韩碧群等: ""雪茶的应用历史与研究现状"", 《中国现代中药》 * |
| 马瑛等: ""藏药雪茶提取物抗疲劳作用的实验研究"", 《中医药学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484583A (en) * | 2019-07-10 | 2019-11-22 | 广西慧宝源医药科技有限公司 | Bird's nest active peptide and its antitumor application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102258442B (en) | Compound traditional Chinese medicine extract and application thereof to whitening, moisturizing and anti-aging skin care product | |
| CN104666236B (en) | A kind of preserving moisture and protecting skin facial film | |
| EP2574332A1 (en) | MC-1 R, MC-2 R, and µ opioid receptors modulation | |
| CN104721129A (en) | Microemulsion biotechnology skin care product | |
| CN103054771A (en) | Ultra-elastic water-locking facial mask essence and preparation method thereof | |
| CN102274141B (en) | Herba selaginellae revival moisturizing cream and preparation method thereof | |
| CN106420531A (en) | Traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function and application | |
| CN108992372B (en) | Moisturizing composition containing snow lotus herb extract and skin care product thereof | |
| CN106798658A (en) | A kind of moisture-keeping composition and skin care item | |
| CN110772454A (en) | Compound essential oil for skin brightening, moisturizing, soothing and anti-aging, and preparation method and application thereof | |
| CN108670933B (en) | Skin care composition with moisturizing, whitening and anti-aging functions and application thereof | |
| CN109864911A (en) | A kind of application for avenging tea extraction | |
| CN106913495A (en) | Profit maintenance skin essence dew and preparation method thereof | |
| EP2629752B1 (en) | Cosmetic or dermatological composition including an angelica extract, and use thereof for moisturisation and radiance | |
| CN105560090A (en) | Skin-activating moisturizing anti-wrinkle face-moisturizing cream | |
| CN111419770A (en) | Moisturizing and repairing type temperature-sensitive awakening facial mask and preparation method thereof | |
| KR102071218B1 (en) | Anti-aging and anti-wrinkle of skin improving cosmetic composition comprising extracts of Moschus, Angelica gigas Nakai, Cornus officinalis, Cervi Parvum Cornu and Phellinus linteus, Ganoderma Lucidum, Inonotus obliquus, Grifola Frondosa, Sparassis crispa | |
| WO2023030041A1 (en) | Type i collagen production accelerant | |
| CN115040448A (en) | Skin care product with enhanced anti-aging effect and preparation method thereof | |
| CN114632045A (en) | Anti-aging composition, preparation method and application | |
| CN115137675A (en) | Compound plant extract with moisturizing effect and application thereof | |
| WO2013188774A2 (en) | Skin care formulations including octapeptide complexes and methods for their manufacture | |
| JP7289833B2 (en) | Cosmetic use of plant Elephant extract (Phytelephas sp.) | |
| CN109498529A (en) | The application of bletilla striata extract | |
| CN109875937A (en) | The application of Herba Dendrobii extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190611 |