CN109846968A - Composition and the application for preventing the bacillary intestines problem of far-reaching extra large cultured fishes - Google Patents
Composition and the application for preventing the bacillary intestines problem of far-reaching extra large cultured fishes Download PDFInfo
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- CN109846968A CN109846968A CN201910042450.XA CN201910042450A CN109846968A CN 109846968 A CN109846968 A CN 109846968A CN 201910042450 A CN201910042450 A CN 201910042450A CN 109846968 A CN109846968 A CN 109846968A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The present invention relates to a kind of composition for preventing the bacillary intestines problem of far-reaching extra large cultured fishes and applications, belong to sea-farming disease prevention techniques field.The composition and mass fraction of the composition are as follows: 20~30 parts of berberine tannate, 8~10 parts of Verbena officinalis ethanol extract, 15~20 parts of the rheum officinale Ultramicro-powder of 200~300 mesh, 200~300 10~15 parts of purpose small ground cypress Ultramicro-powders, colored 10~15 parts of Ultramicro-powder of 200~300 purposes three, 10~15 parts of K-type carragheen, 1~2 part of calcium citrate.Using the pharmaceutical chemistry of the bacillary intestines problem of the composition far-reaching extra large cultured fishes of preparation prevention, the bacillary intestines problem that can effectively prevent far-reaching extra large cultured fishes with the pharmaceutical chemistry 5~7 days was fed every 15~20 days compared with Gao Shiying in summer and autumn water temperature.
Description
Technical field
The invention belongs to sea-farming disease prevention techniques field, to prevent far-reaching extra large cultured fishes thin more particularly to a kind of
It the composition of bacterium property intestines problem and answers.
Background technique
Bacillary intestines problem is most commonly seen one of the disease of fish, and almost all of sea, cultured freshwater fish all may be used
Intestines problem occurs.The fish of infection intestines problem are usually expressed as vigor decline, do not like to ingest, slow growth when symptoms are mild
Deng as the development of the state of an illness can cause the other symptoms such as ascites, tissue fester, leading to the functional failure of internal organs, finally make
At fish death.The death rate that serious intestines problem even can cause in a short time cultured fishes high, is to endanger fish
One of the disease of class aquaculture industry most serious.In breeding process, there are many reason of causing fish intestines disease, but in most cases
It is to be passed transorally into infection by pathogen and fall ill.For seawater fish, Vibrio anguillarum, Vibrio splindidus, Vibrio harveyi, big water chestnut
A variety of pathogenetic bacterias such as flounder vibrios, Mermaid luminous bacillus, Edwardsiella tarda can infect different fish and occur
Intestines problem.Therefore, the incidence more universal one of intestines problem caused by during marine fish culture because of bacterium infection
A bit.
Compared to research the bacillary intestines problem of cultured fishes occurrence cause for, how it is scientific and effective prevention and control
Treating bacillary intestines problem is one of the technology that breeding production more needs.In traditional cultivation experience, oral antibiotic quilt
It is considered to prevent and treat maximally efficient one of the mode of fish bacterial intestines problem.But as more and more antibiotic are supported in aquatic products
The continuous improvement that disabled and consumer requires aquaculture product Safety of Food Quality in growing is found safer, environmentally friendly
Substitutes For Antibiotic become vast aquatic products practitioner most for preventing and treating the bacillary intestines problem of fish during breeding production
For one of focus of attention problem.
With the fast development of China's mariculture industry, the problems during industry development also gradually show, the most
Outstanding is exactly land and seawater fishery space compression, and the development of traditional sea-farming mode is restricted.It is expanded to off-lying sea
Breeding spaces become the Main way of sea-farming industry development.Fish are suitable for that development is far-reaching because of its specific biological habit
The various modes cultivation such as extra large wind wave-resisting net casing, descending cylinder mould, purse seine, cultivation work ship.And these aquaculture models belong to high
Density culture.From the point of view of historical experience according to China's sea-farming industry development process, high density, intensive aquaculture model exist
The restriction of disease is not often got rid of during development, especially bacteroidal intestines problem is more universal.And far-reaching sea is supported
It grows because of its cultivation site from coastline farther out and the factors such as the cultivation depth of water, cannot achieve the real-time observation to cultured fishes, this
To find that disease causes certain difficulty in time.Therefore, developing a kind of can effectively prevent far-reaching extra large cultured fishes intestines
The technical method that tract disease occurs is with regard to very necessary.
Summary of the invention
Technical problem to be solved by the invention is to provide one kind can effectively prevent the bacillary intestines of far-reaching extra large cultured fishes
The composition of tract disease and its specific application method, by regularly using being killed in far-reaching extra large cultured fishes enteron aisle in time
Harmful bacteria venereal disease is former, and effective bacillary intestines problem of prevention and control occurs, and avoids using antibiotic, for far-reaching extra large cultured fishes
Food safety provides technical guarantee.
The present invention is realised by adopting the following technical scheme:
A kind of composition preventing the bacillary intestines problem of far-reaching extra large cultured fishes, composition and mass fraction are as follows:
20~30 parts of berberine tannate, 8~10 parts of Verbena officinalis ethanol extract, 15~20 parts of the rheum officinale Ultramicro-powder of 200~300 mesh, 200
~300 10~15 parts of purpose small ground cypress Ultramicro-powders, colored 10~15 parts of Ultramicro-powder of 200~300 purposes three, K-type carragheen 10~
15 parts, 1~2 part of calcium citrate.
Further, the composition of the bacillary intestines problem of the far-reaching extra large cultured fishes of prevention, utilizes the composition
Prepare the method for feed pharmaceutical bait the following steps are included:
(1) its dosage is every 1kg feed addition 15~20g composition;
(2) according to the ratio of every 100g composition addition 800~1000mL fresh water, phase is weighed according to forage volume to be fed
Weighed composition is proportionally added into freshwater soaking 10min or more by the composition that should be measured, then with being boiled with soft fire 3~5min;
(3) solution high pressure watering can or spray gun that step (2) has been boiled are sprayed to rapidly before cooling to be fed
In feed;It needs to stir solution at any time during sprinkling, avoids generating a large amount of precipitatings, while should spray on one side, agitation is raised on one side
Material guarantees that composition solution can be uniformly sprayed on all feeds;
(4) sprayed feed should continue uniformly to stir 10min or more, up to composition solution cooled and solidified and adhere to
In feed surface;
(5) feed with composition made is fed immediately, or is stored in freezer and is fed in batches.
Further, the volume of solution is not less than the fresh water volume being initially added after the completion of step (2) is boiled.
For the bacillary intestines problem for effectively preventing far-reaching extra large cultured fishes, summer and autumn water temperature compared with Gao Shiying every 15~
The feed fed with composition for 20 days 5~7 days.
The beneficial effect of the present invention compared with the prior art:
It is easy 1. composition composition of the present invention obtains, is cheap, being suitable for that aquaculture uses.
2. composition of the present invention is free of antibiotic, medicament residue problem will not be generated, can effectively ensure that far-reaching sea is supported
The Safety of Food Quality of breeding fish.
3. the method for the invention is effectively combined drug ingedient with gel composition, make the method letter for making pharmaceutical chemistry
It is single, efficient, and manufactured pharmaceutical chemistry drug ingedient and bait close adhesion, it is not easy to lose.
4. using the method for the invention, pharmaceutical chemistry can also produce once more batches and be stored for a long time, effectively improve existing
There is off-lying sea cage culture pharmaceutical chemistry production process cumbersome, and need now to do used technology drawback, reduces man power and material's investment,
It is very suitable to the breeding way in far-reaching sea.
Specific embodiment
Technical solution of the present invention is described in detail below by embodiment:
Embodiment 1
First in the Shui nationality experimental system in this laboratory, pharmaceutical chemistry production is carried out using the method for the invention and has been fed
The experiment of the flat Rockfish of Xu Shi.Experiment uses the far-reaching extra large aquaculture net cage of Glass fibre reinforced plastic tub simulation of 6 diameters 90cm, high 110cm.Wherein
3 buckets are set as A group, the seawater of 500L and the flat Rockfish juvenile fish of Xu Shi of 50 tail average weight 40g or so are respectively provided in bucket, and adopt
With open flowing water culture mode.Other 3 buckets are set as B group, the seawater of 500L are only housed in bucket, and use pipeline mode.
Composite formula used by testing and mass fraction are as follows: 30 parts of berberine tannate, Verbena officinalis ethanol extract 10
Part, 20 parts of the rheum officinale Ultramicro-powder of 200~300 mesh, 200~300 10 parts of purpose small ground cypress Ultramicro-powders, 200~300 flowers of purpose three
15 parts of Ultramicro-powder, 13 parts of K-type carragheen, 2 parts of calcium citrate.
Specific experiment method are as follows: (1) pellet of 5kg is put into plastic barrel first.(2) above-mentioned composition is weighed
100g is put into another large beaker, be added 1L freshwater soaking 20min after, then be boiled with soft fire and keep boiling 5min.It boils
After boiling, supplement fresh water makes final liquor capacity not less than 1L.(3) the 1L composition solution boiled is poured into rapidly 1
In a high pressure watering can, the pellet into plastic barrel is sprayed, and is stirred continuously with stirring rod.It is shaken at any time during sprinkling
Dynamic watering can, avoids the formation of precipitating.(4) feed after sprayed continues after stirring 10min, then spreads out and dry 30min or more, directly
It is solidified completely to gel, composition components is closely adhered to diet surface.
First by 3 buckets of the pharmaceutical chemistry made investment B group, each barrel of investment 50g observes 1 hour.Pass through observation
It was found that 50g pharmaceutical chemistry can be stabilized 1h or more in 3 buckets, bait has no decomposition, and composition composition also has no dissolution.With
Afterwards, then take 150g to make 3 buckets of bait feeding A group in Xu Shi flat Rockfish, each barrel of investment 50g, and the interior throwing in 5min
It feeds and finishes.Feed in experimentation, the flat Rockfish of Xu Shi in 3 buckets robs dyspepsia pole, in 10min will all pellets ingest
It finishes.
Pass through this test, it was demonstrated that the method for the invention can effectively by drug ingedient be adhered to pellet show and
It can guarantee that drug ingedient is ingested by the flat Rockfish of Xu Shi completely, do not lose or dissolve out, substantially increase pharmaceutical chemistry feeds efficiency.
Embodiment 2
Shandong Province, current -resistance submerged cage cultivation base, Changdao County, the large-scale circular using perimeter 40m, underwater depth 7m are anti-
Stormy waves cage culture Xu Shi flat more than ten years of Rockfish.But from 2010, cultivated in annual July~October higher period net cage of water temperature
The flat Rockfish of Xu Shi will large area generation enteritis disease, wherein 2014~2016 years discovery infection enteritis disease aquaculture net cage be more than
60%, every mouthful of net cage day the death rate 0.03%~0.5%, the death time can continue 3 months or more, cause to raiser
Serious economic loss.Pass through epidemiological survey and Study on etiology in 2 years, it was demonstrated that the culture zone flat Rockfish's of Xu Shi is thin
Bacterium property enteritis disease is mainly caused by Mermaid luminous bacillus and wheel animalcule vibrio infection.
2017,5 mouthfuls of net cages are had chosen in the cage culture area, the flat Rockfish bacterium of Xu Shi is carried out using the method for the invention
The prevention and treatment of property enteritis disease.Used composite formula and mass fraction are as follows: 30 parts of berberine tannate, Verbena officinalis ethanol extract
10 parts, 20 parts of the rheum officinale Ultramicro-powder of 200~300 mesh, 200~300 10 parts of purpose small ground cypress Ultramicro-powders, 200~300 purposes three
15 parts of Ultramicro-powder of flower, 13 parts of K-type carragheen, 2 parts of calcium citrate.
Specific application method are as follows: (1) first the pellet of 50kg Xu Shi flat Rockfish is put into a big stainless steel barrel,
Stirring motor and stirring rod are set up on bucket.(2) above-mentioned composition 1kg is weighed, after 10L water immersion 20min is added, boils and keeps
Boil 5min.During boiling, fresh water is supplemented at any time, makes final liquor capacity not less than 10L.(3) it will boil
10L composition solution pours into rapidly in 2~3 high pressure watering cans, respectively the pellet from 2~3 people while into stainless steel barrel
It is sprayed, opens simultaneously stirring motor and start to stir.High pressure watering can is shaken during sprinkling at any time, avoids the formation of precipitating, together
When sprinkling should be completed as early as possible before solution is cooling, prevent solution from solidifying.(4) after 10L composition solution is all sprayed,
Continue to turn off after being kept stirring motor operating 30min, gel solidifies completely, and composition components are closely adhered to bait table
Face.(5) pellet containing the present composition made is dispensed according to daily feeding volume, is put into freezer and protects
It deposits stand-by.(6) since in mid-June, 2017, according to pharmaceutical chemistry 7 days made are fed every time, every 20 days are fed 7 days again
The scheme of pharmaceutical chemistry prevents experiment in the intestines problem that 5 mouthfuls of selected net cages carry out the flat Rockfish of Xu Shi, until stopping mid-October feeding
Pharmaceutical chemistry.
By the above measure, the flat Rockfish of Xu Shi of 5 mouthfuls of selected cage cultures of this experiment is in mesosome in July, 2017~October
Matter health is ingested actively, and there is no large-scale intestines problems, and extremely a other fragmentary death only has occurred.And periphery net cage
Large-scale intestines problem has occurred again between 8~September, wherein the aquaculture net cage cumulative mortality of disease hair most serious is more than
70%, cause serious economic loss.Illustrate composition and its application method of the present invention at this by comparative experiments
Deep-water net cage culture sea area has reached the preventive effect of very good fish bacterial intestines problem.
Above-described embodiment is the optimal embodiment of technical solution of the present invention, other in technical solution of the present invention composition
Mass parts ratio can achieve the effect that the bacillary intestines problem of the far-reaching extra large cultured fishes of prevention.
Claims (3)
1. a kind of composition for preventing the bacillary intestines problem of far-reaching extra large cultured fishes, it is characterised in that the composition of the composition
Composition and mass fraction are as follows: 20~30 parts of berberine tannate, 8~10 parts of Verbena officinalis ethanol extract, the rheum officinale of 200~300 mesh
15~20 parts of Ultramicro-powder, 200~300 10~15 parts of purpose small ground cypress Ultramicro-powders, the colored Ultramicro-powder of 200~300 purposes three 10~
15 parts, 10~15 parts of K-type carragheen, 1~2 part of calcium citrate.
2. preventing the feed pharmaceutical bait of the bacillary intestines problem of far-reaching extra large cultured fishes using pre-composition preparation described in claim 1
Method, it is characterised in that the described method comprises the following steps:
(1) dosage of composition is every 1kg feed addition 15~20g composition;
(2) according to the ratio of every 100g composition addition 800~1000mL fresh water, corresponding amount is weighed according to forage volume to be fed
Composition, weighed composition is proportionally added into freshwater soaking 10min or more, then with being boiled with soft fire 3~5min;
(3) solution that step (2) has been boiled is sprayed to rapidly feed to be fed with high pressure watering can or spray gun before cooling
In;It needs to stir solution at any time during sprinkling, avoids generating a large amount of precipitatings, while should spray on one side, stir feed on one side, protect
Card composition solution can be uniformly sprayed on all feeds;
(4) sprayed feed should continue uniformly to stir 10min or more, up to composition solution cooled and solidified and be adhered to feeding
Expect surface;
(5) feed with composition made is fed immediately, or is stored in freezer and is fed in batches.
3. according to the method described in claim 2, it is characterized in that the volume of solution is not less than after the completion of the step (2) is boiled
The fresh water volume being initially added.
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