CN109846927A - A kind of Chinese medicine composition for treating the reaction of essential hypertension complicated vessel wall inflammation - Google Patents
A kind of Chinese medicine composition for treating the reaction of essential hypertension complicated vessel wall inflammation Download PDFInfo
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Abstract
The present invention provides a kind of Chinese medicine compositions for treating essential hypertension complicated vascular wall inflammation, it is prepared by the bulk pharmaceutical chemicals of following weight proportion: 10-60 parts of Prunella vulgaris, 5-30 parts of radix scutellariae, 10-60 parts of wild chrysanthemum, 5-30 parts of Cortex Eucommiae, 10-60 parts of Eclipta prostrata, 5-30 parts of pheretima, 10-60 parts of pueraria lobata, 5-30 parts of Radix Salviae Miltiorrhizae, 5-30 parts of radish seed, 5-30 parts of radix achyranthis bidentatae.The present invention also provides the preparation method of the Chinese medicine composition and purposes.Chinese medicine composition provided by the invention has the benefits of tonifying the liver and kidney, clearing liver-fire, demonstrate,proves and sets for high blood pressure kidney deficiency liver-yang hyperactivity, meets hypertension with the characteristics of incidence of vascular wall chronic inflammation, has good therapeutic effect；The composition can be obviously improved patient clinical symptom, such as headache, dizziness, insomnia, tinnitus, dysphoria while decompression, improve the life quality of patient, provide a kind of new medicament selection for clinic.
Description
Technical field
The invention belongs to drug fields, and in particular to a kind of Chinese medicine for treating the reaction of essential hypertension complicated vessel wall inflammation
Composition.
Background technique
In recent years epidemiological study confirms that essential hypertension (Essential hypertension, EH) patient exists
Vessel wall inflammation reaction, inflammatory reaction participate in the pathophysiological process of essential hypertension, are the important rings that high blood pressure is formed
Section.EH is considered as a kind of systemic inflammatory response state disease of minuent in the early stage, characterized by low lasting inflammation,
It is emerged from blood vessel endothelium dysfunction, and the later period of EH progress, target organ damage take place, inflammatory factor is in target
Expression increased significantly in organ-tissue.Tumor necrosis factor α (TNF-α), interleukins (IL-6), c reactive protein (CRP) are
There are higher several inflammatory factors in Hypertensive Population body, and reflect the main indicator of its chronic inflammation.Inflammation is mutual with EH
It mutually influences, reciprocal causation, the relationship of one vicious circle of shape.How while decompression, mitigate inflammation, protect blood vessel endothelium,
To prevent or delay target organ damage to have become the hot spot of EH research.
With spectrum of disease and living-pattern preservation, clinical prevention disease and people's health care concepts are also occurring
Variation, it has been found that taking doctor trained in Western medicine depressor for a long time will cause the damage of the target organs such as patient's heart, brain, kidney, cause " drug induccd disease
Disease ".And in terms of Chinese medicine, relevant rudimentary and the own confirmation traditional Chinese medicine of clinical research not only have specific antihypertensive effect, can more drop
Low CRP, IL-6, the expression of the inflammatory factors such as TNF- ɑ, thus inhibit hypertension with chronic inflammatory reaction, traditional Chinese medicine institute
The multiple target points advantages such as the decompression, anti-inflammatory, the tune rouge that have are to treat hypertension with traditional Chinese medicine to reach the overall cardiovascular risk of reduction
Target provide possibility.
But the research in terms of not having the Chinese medicine composition for the treatment of essential hypertension complicated vessel wall inflammation reaction also at present
Report.
Summary of the invention
To solve the above-mentioned problems, it is treated in the reaction of essential hypertension complicated vessel wall inflammation the present invention provides a kind of
Drug composition, it is characterised in that: it is prepared by the bulk pharmaceutical chemicals of following weight proportion:
10-60 parts of Prunella vulgaris, 5-30 parts of radix scutellariae, 10-60 parts of wild chrysanthemum, 5-30 parts of Cortex Eucommiae, 10-60 parts of Eclipta prostrata, pheretima
5-30 parts, 10-60 parts of pueraria lobata, 5-30 parts of Radix Salviae Miltiorrhizae, 5-30 parts of radish seed, 5-30 parts of radix achyranthis bidentatae.
Further, it is prepared by the bulk pharmaceutical chemicals of following weight proportion:
30-40 parts of Prunella vulgaris, 10-20 parts of radix scutellariae, 15-40 parts of wild chrysanthemum, 15-20 parts of Cortex Eucommiae, 15-40 parts of Eclipta prostrata,
15-20 parts imperial, 30-40 parts of pueraria lobata, 15-30 parts of Radix Salviae Miltiorrhizae, 10-20 parts of radish seed, 10-20 parts of radix achyranthis bidentatae.
Further, it is prepared by the bulk pharmaceutical chemicals of following weight proportion:
30 parts of Prunella vulgaris, 10 parts of radix scutellariae, 15 parts of wild chrysanthemum, 15 parts of Cortex Eucommiae, 15 parts of Eclipta prostrata, 15 parts of pheretima, 30 parts of pueraria lobata,
15 parts of Radix Salviae Miltiorrhizae, 12 parts of radish seed, 15 parts of radix achyranthis bidentatae.
Further, it be with Prunella vulgaris, radix scutellariae, wild chrysanthemum, Cortex Eucommiae, Eclipta prostrata, pheretima, pueraria lobata, Radix Salviae Miltiorrhizae, radish seed,
The primary medicinal powder or water extract of radix achyranthis bidentatae are active constituent, and pharmaceutically acceptable auxiliary material or the preparation of complementary ingredient is added
Made of preparation.
Further, the preparation is oral preparation.
Further, the oral preparation is paste, pill, oral solution, powder, tablet, granule or capsule.
The present invention also provides a kind of methods for preparing aforementioned Chinese medicine composition, it is characterised in that: it includes the following steps:
A, the raw material of aforementioned weight proportion is weighed；
B, bulk pharmaceutical chemicals beat powder, or bulk pharmaceutical chemicals are added water to cook, water cooking liquid concentration, add pharmaceutically acceptable auxiliary material or
Complementary ingredient is prepared into pharmaceutically common preparation.
The present invention also provides a kind of purposes of aforementioned Chinese medicine composition in the drug of preparation treatment vascular wall inflammation.
The present invention finally provides a kind of use of aforementioned Chinese medicine composition in the drug of preparation treatment essential hypertension
On the way.
Further, the drug is the drug for treating the reaction of essential hypertension complicated vessel wall inflammation.
Chinese medicine composition of the invention is not but to match in Chinese prescription simply by the Chinese medicinal composition with antihypertensive effect
Forefathers' practical experience and doctor trained in Western medicine pharmacological research, compatibility is combined to form under 5 theoretical guidances.Three complements each other, to promote this
Pharmaceutical composition more preferably plays the effect of anti-inflammatory decompression.
Chinese medicine composition of the present invention, each drug pharmacological effect are respectively as follows:
Prunella vulgaris: acrid flavour, hardship are trembled with fear；Return liver, gallbladder channel.With clearing liver-fire, the effect of improving eyesight, mass dissipating and swelling eliminating.It is mainly used for
Red eye, swell pain, eyeball night pain, headache and dizziness, scrofula, goiter, acute mastitis, breast illness, the illnesss such as swollen breasts.Modern pharmacology research
Show, Prunella vulgaris has the pharmacological actions such as antitumor, anti-inflammatory, antibacterial, antiviral, blood pressure lowering, hypoglycemic, the immune, liver protection of adjusting；
There are very strong resistant function, extract and active constituent sieve danshinolic acid to mitigation vascular inflammation and periodontal disease Earlier period of inflammation
Deng all having certain effect, great inhibiting effect is also shown to specific immunity.
Radix scutellariae: taste bitter, it is cold in nature；Return lung, gallbladder, spleen, large intestine, small intestinl channel.With heat-clearing and damp-drying drug, purging fire for removing toxin is stopped blooding, peace
The effect of tire, cure mainly damp-warm syndrome, summer-heat and damp, chest tightness and emesis, damp and hot feeling of fullness, dysentery, jaundice, cough with lung heat, hyperpyrexia polydipsia, blood-head spits
Nosebleed, carbuncle sore tumefacting virus, the illnesss such as threatened abortion.It is antipyretic, anti-inflammatory, antimicrobial, anti-swollen that modern pharmacology research shows that radix scutellariae has
The pharmacological actions such as tumor, anti-oxidant have certain therapeutic effect to diseases such as digestive system, cardiovascular system, nervous systems.
Wild chrysanthemum: it is bitter, pungent, it is slightly cold；Return liver, the heart channel of Hang-Shaoyin.With clearing heat and detoxicating, the effect of purging intense heat calming the liver.Cure mainly furunculosis carbuncle
It is swollen, red eye, swell pain, the illnesss such as headache and dizziness.Modern pharmacology is it is demonstrated experimentally that wild chrysanthemum water extract and volatile oil not only have significantly
Anti-inflammatory, immunosuppressive action, there are also apparent antihypertensive effects, can inhibit blood by antiadrenergic drug and expansion peripheral blood vessel
Pipe motor center is depressured.
Cortex Eucommiae: sweet in flavor micro- acrid in flavour and warm in nature；Enter liver and kidney channel.With nourishing liver and kidney, the effect of strengthening the bones and muscles, miscarriage prevention.Spinal column ache is cured mainly,
Foot and knee flaccidity, dribbling urination, uncontrolled urination, vaginal bleeding during pregnancy are intended to fall, threatened abortion, the illnesss such as hypertension.Modern research shows that Cortex Eucommiae contains
There are many active constituents, have the effects that decompression, diuresis, antibacterial, anti-inflammatory, calm, hemostasis, analgesia；Its antihypertensive effect is steady, no
Good reaction is small.
Eclipta prostrata: acid sweet in flavor, it is cool in nature；Enter liver kidney two warp.All herbal medicine has cool blood, hemostasis, detumescence, strong function
Effect.Cure mainly various haematemesis, nosebleed, hemoptysis, enterorrhagia, hematuria, hemorrhoid hemorrhage, the diseases such as metrorrhagia.Modern pharmacology research shows,
Eclipta have the bioactivity such as hemostasis, liver protection, immunological regulation, antitumor, anti-inflammatory, be clinically mainly used for treatment coronary heart disease,
Hemorrhagic disease etc..
Pheretima: taste is salty, trembles with fear；Return liver, spleen, bladder meridian.Have effects that heat-clearing arresting convulsion, dredging collateral, relieving asthma, diuresis.Normal frying
Afterwards for illnesss such as high fever, coma, twitch, arthralgia, dyspnea and cough due to lung-heat, oliguria oedema, hypertension.Modern studies have found that
Pheretima has the effects of antithrombotic, antitumor, adjusting is immune, decompression anti-arrhythmia, easing pain and diminishing inflammation；Ground dragon protein can be different degrees of
The diastolic pressure and systolic pressure of ground reduction spontaneous hypertensive rat.
Pueraria lobata: it is sweet in flavor, pungent, it is cool；There is expelling pathogenic factors from muscles and skin to bring down a fever, promoting eruption promotes the production of body fluid to quench thirst, the function of Shengyang Zhixie.It is usually used in exterior syndrome fever,
Stiff nape and back, measles without adequate eruption, pyreticosis is thirsty, and the deficiency of Yin is quenched one's thirst, and heat is purged heat dysentery, the illnesss such as splenasthenic diarrhea.Modern pharmacology research table
Bright, Puerarin has decompression, adjusts rouge, improve blood flow variation, alleviate platelet aggregation and anti-inflammatory pharmacological action；Puerarin is to height
The cardiovascular diseases such as blood pressure, angina pectoris, myocardial infarction can also play anti-inflammatory effect.
Radix Salviae Miltiorrhizae: bitter, cold nature；Converge to heart and liver channels.With blood circulation, stasis-dispelling and pain-killing, relieving restlessness and restlessness, cool blood to disappear carbuncle it
Effect is known as activating microcirculation and removing stasis medicinal key medicine.Modern pharmacological studies have shown that Radix Salviae Miltiorrhizae has hypoglycemic, protection vascular endothelial cell, anti-fibre
The effects of dimensionization, antiatherosclerosis, immune enhancing.
Radish seed: acrid flavour, sweet and neutral；Return lung, spleen, stomach meridian.With help digestion and eliminate flatulence, the function of lowering the adverse-rising QI to resolve phlegm.Diet is cured mainly to stop
It is stagnant, abdominal distention, constipation, stagnation and diarrhea, the illnesss such as phlegm choking, dyspnea and cough.Modern research shows that radish seed it is certain digestion, exhale
It inhales and the treatment of circulation system disease aspect has significant effect, and have the pharmacological activity such as anticancer, antihypertensive effect.
Radix achyranthis bidentatae: it is bitter, sweet, sour, it is mild-natured；Return liver and kidney channel.With liver-kidney tonifying, strengthening muscles and bones, degrading the channel, dispelling malignant blood
Function.Clinic is usually used in treating cold-dampness, waist and knee bone pain, soreness and weakness of waist and knees, spasm of limbs, irregular menstruation, postpartum blood stasis abdomen pain, blood
The diseases such as leaching, traumatic injury.Modern pharmacological studies have shown that radix achyranthis bidentatae has significant immunological regulation, anti-aging, anti-inflammatory, analgesia and resists
The effects of tumour, antiatherosclerosis.Especially in terms of cardiovascular system, radix achyranthis bidentatae has temporary expansion to make blood vessel
With the blood vessel of lower limb can be expanded；Also has the function of antiatherosclerosis.
The present invention has anti-inflammatory decompression, adjusts rouge, improves blood circulation, the effect of antithrombotic and antiatherosclerosis.?
It can be by inhibiting the expression of inflammatory factor to be gradually depressured, after there is good effect, blood pressure to decline not in terms for the treatment of essential hypertension
It rebounds, have no toxic side effect, the technical advantage without pharmacological dependence effect.Formula of the invention is carried out in strict accordance with drug matching principle
Compatibility, the incompatibility of no eighteen incompatibilities, nineteen counteraction.In addition, present invention production is simple, cheap, it is suitable for a wide range of promote
It uses, there is preferable medical prospect.
Obviously, above content according to the present invention is not being departed from according to the ordinary technical knowledge and customary means of this field
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific embodiment of form by the following examples remakes further specifically above content of the invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on above content of the present invention
The technology realized all belongs to the scope of the present invention.
Specific embodiment
Raw material, equipment used in the specific embodiment of the invention are known product, are obtained by purchase commercial product.
The preparation of the pharmaceutical composition of the present invention of embodiment 1
Weigh Prunella vulgaris 30g, radix scutellariae 15g, wild chrysanthemum 30g, Cortex Eucommiae 15g, Eclipta prostrata 30g, pheretima 15g, pueraria lobata 30g, pellet
Join 15g, radish seed 15g, radix achyranthis bidentatae 15g
Medicinal material: being first put into casserole by preparation method, and the water of 2000ml is added, and impregnates half an hour.Then (big with intense fire
Fire) boil after, then surplus 200-250ml boiled with mild fire (small fire), well-done medical fluid poured out in bowl, the dregs of a decoction decoct again with method
Twice, merge medical fluid.
The preparation of the pharmaceutical composition of the present invention of embodiment 2
Weigh Prunella vulgaris 40g, radix scutellariae 20g, wild chrysanthemum 40g, Cortex Eucommiae 20g, Eclipta prostrata 40g, pheretima 20g, pueraria lobata 40g, pellet
Join 20g, radish seed 20g, radix achyranthis bidentatae 20g
Preparation method: with the preparation method of embodiment 1.
The preparation of the pharmaceutical composition of the present invention of embodiment 3
Weigh Prunella vulgaris 30g, radix scutellariae 10g, wild chrysanthemum 15g, Cortex Eucommiae 15g, Eclipta prostrata 15g, pheretima 15g, pueraria lobata 30g, pellet
Join 15g, radish seed 12g, radix achyranthis bidentatae 15g.
Preparation method: with the preparation method of embodiment 1.
The preparation of 4 present composition of embodiment
Formula: with embodiment 3.
Preparation process: weighing each bulk pharmaceutical chemicals by formula, crush, be sieved, be uniformly mixed, packing to get.
Beneficial effects of the present invention are illustrated below by way of test example:
1 typical case of test example
Lee, male, 56 years old.Patient's readme: having hypertension history 6 years, the drop such as informal dress " compound Herba Apocyni veneti (Folium Apocyni Veneti) ", " captopril "
Pressing, blood pressure are unstable always；Recently feel and have a dizzy spell, very dizzy, dynamic then dizziness is vomitted, with tinnitus, bitter taste dry, waist and knee acid
Soft, urine is yellow, and stool does not solve for three days.It is sturdy partially fat to look into its body, lustrous eyes, appearance is red red, and tongue nature is slightly red, and yellow tongue fur is thin
It is greasy, stringy,thready and rapid pulse, blood pressure: 165/105mmHg.
Take 1 Chinese medicine composition decoction of embodiment, instructions of taking: three times a day, about 200mL, warm are taken every time, and 7 days
One course for the treatment of.After taking a course for the treatment of, patients' blood falls to 140/100mmHg, and stool 2-3 urine of day is increased, and all diseases are big
Subtract, dizziness is not vomitted, and soreness and weakness of waist and knees mitigates.Three courses for the treatment of are taken again, and patient consciously withdraws Chinese medicine without discomfort.
Chen, male, 70 years old, suffers from more than 10 years of hypertension history, the headache of private prosecution alliteration, being irritable and getting angry easily, amnesia tinnitus, waist and knee
Aching and limp, drugs, the state of an illness such as clothes " enalapril maleate " are good or bad, and start to take 2 Chinese medicine group of embodiment in August, 2017
Two courses for the treatment of of object are closed, instructions of taking: three times a day, about 200mL, warm are taken every time, 7 days courses for the treatment of.After symptom mitigation again
Continuously take two courses for the treatment of.Follow-up 1 year, stable disease was without repeatedly.
Mr. Lin: male, 68 years old, retired cadre, in March, 2016 was medical, suffered from hypertension combination of syndromes hyperlipidemia for many years, and suffer from rouge
Many complication such as fat liver, 3 Chinese medicine composition of the embodiment of the present invention by oral administration, one dose per day, three times a day, about 200mL every time,
Warm is taken, 7 days courses for the treatment of, and after taking four courses for the treatment of, blood pressure decline continues blood lipids index after taking four courses for the treatment of and restores just
Often；Interruption takes four courses for the treatment of again afterwards, and after 3 months, blood fat and blood pressure restores normal, and dizziness, fatty liver etc. are eliminated.
Huang, male, 55 years old, hypertensive patient was closely often accompanied by headache, irritable, soreness and weakness of waist and knees, dry, face over the past half year
Red, reddish tongue tongue fur is thin slightly yellow, and deep wiry pulse, chi pulse is weak, takes the decoction of 3 Chinese medicine composition of the embodiment of the present invention, one dose per day,
Three times a day, about 200mL, warm are taken every time, 7 days courses for the treatment of, after taking three courses for the treatment of, dizzy, headache, irritable and
Soreness and weakness of waist and knees is substantially reduced, measuring blood pressure 140/93mmHg, and after continuing taking the two courses, blood pressure restores normal, without any bad
Symptom.
Zhao, female, 63 years old, hypertensive patient, frequent dizziness, palpitaition, insomnia, measuring blood pressure 165/112mmHg, tongue nature
Slightly red, tongue fur is thin slightly yellow, deep wiry pulse, takes the traditional Chinese herbal decoction of the embodiment of the present invention 3, one dose per day, three times a day, every time about
200mL, warm are taken, 7 days courses for the treatment of, and after taking two courses for the treatment of, dizzy, palpitaition is clearly better, and blood pressure is down to 140/
105mmHg continues to take three courses for the treatment of, and all diseases are lost entirely, and blood pressure restores normal.
2 animal effect experiment of test example
1. materials and methods
1.1 test animal
Healthy spontaneous hypertensive rat (SHR) 20,15 week old, SPF grades, weight range 200-280g, male,
It tests Company of Animals Ltd. up to rich fruit by Chengdu to provide, quality certification number: SCXK (river) 2015-030.
Healthy Wistar-Kyoto rat (WKY) 4,15 week old, SPF grades, weight range 180-200g, SPF grade,
Male is tested Company of Animals Ltd. up to rich fruit by Chengdu and is provided, quality certification number: SCXK (river) 2015-030.
Rat is fed in Chengdu University of Traditional Chinese Medicine's laboratory animal room, divides cage to feed, 4, every cage, totally 6 cage, animal housing are
Cleaning grade, humidity (55 scholar 5%), environment temperature (22 10 DEG C of scholar), light and shade period 12h/12h, common special feed are fed, often
Rat drinking water and addition feed are replaced in its timing clean animal room.It adapts to environment 1 week, observes the spirit of rat, moving type
Condition, and its abnormal conditions activity is recorded, activity, excrement etc. are tested after being showed no exception.
1.2 experimental drugs and preparation
The traditional chinese medicine composition of the invention kidney tonifying clearing liver soup: it prepares, then is concentrated as every milliliter containing about life according to the method for embodiment 1
The soup of medicine 2.1g, plastic soft bag seal bag are placed in stored refrigerated in 3-5 DEG C of refrigerator, nursing taking-up in first 30 minutes, sufficiently
It shakes up, to prevent precipitating.Composition: Prunella vulgaris 30g, radix scutellariae 15g, wild chrysanthemum 30g, Cortex Eucommiae 15g, Eclipta prostrata 30g, pheretima 15g, pueraria lobata
30g, Radix Salviae Miltiorrhizae 15g, radish seed 15g, radix achyranthis bidentatae 15g.
The preparation of enalapril suspension: enalapril maleate 10mg/ piece, the limited public affairs of Hunan a thousand pieces of gold Xiang River medicine company share
It takes charge of (national drug standard number: H20066383).Tablet is ground and adds distilled water for smalls, the suspension of the concentration of 10ml/kg containing crude drug is made
Liquid.
10% chloraldurate: it is purchased from Shanghai Ji Taiyikesai Biotechnology Co., Ltd.
1.3 instruments and reagent
FA1104 medical electric balance, big rat-tail pressure heart rate measurement instrument (Chengdu University of Traditional Chinese Medicine) of MRBP type, JZ-HS high
Fast refrigerated centrifuge (Chengdu University of Traditional Chinese Medicine), rat oral gavage device is several, each 6, scissors for surgery, knife, takes blood vessel several, tweezer
Subnumber, syringe 8, sterile gloves number pair, weighing paper number, filter paper number, gauze, volumetric flask etc..
Rat hs-CRP kit, tumor necrosis factor kit, interleukin-6 kit, be purchased from
Shanghai Ji Taiyikesai Biotechnology Co., Ltd.
1.4 experimental group
1. SHR rat is divided into 5 groups, every group 4 according to random digits table.Respectively kidney tonifying clearing liver soup high dose group,
Middle dosage, low dose group, enalapril group and model control group.
2. blank control group: 4 WKY rats.
1.5 administration
After adaptive feeding one week without exception, start gastric infusion, the high, medium and low dosage group in kidney tonifying clearing liver side is given respectively
Concentration is 43.75g/kg/d, 21.87g/kg/d, 10.93g/kg/d, awards medical fluid 1ml with liquid by the every 100g weight of rat, gives
Medicine approach is stomach-filling.Successive administration 42 days, once a day, administration time was 9 points of every morning.
Kidney tonifying clearing liver high dose group: 43.75g/kg/d stomach-filling, once a day, continuous 42 days are pressed with kidney tonifying clearing liver side.
Kidney tonifying clearing liver middle dose group: 21.87g/kg/d stomach-filling, once a day, continuous 42 days are pressed with kidney tonifying clearing liver side.
Kidney tonifying clearing liver low dose group: 10.93g/kg/d stomach-filling, once a day, continuous 42 days are pressed with kidney tonifying clearing liver side.
Enalapril maleate group: giving enalapril suspension, is made into 10ml/kg stomach-filling (containing according to that by weight standard
Puli 3.5mg/ml)
Model control group: physiological saline, stomach-filling 10ml/kg are given.
Blank control group: physiological saline, stomach-filling 10ml/kg are given.
1.6 Indexs measure
1.6.1 arteria caudalis blood pressure detecting:
After experiment starts, heart rate measurement instrument, the tail systolic arterial pressure of indirect determination rat are pressed with the big rat-tail of MRBP type
(SBP), guarantee that rat under quiet waking state, surveys its systolic pressure, measurement three times, takes its average value as the blood of the rat
Pressure.Before administration, administration latter weekend, two weekends, four weekends, six weekends respectively measure once.
1.6.2 Serum hs-CRP, TNF-a, IL-6 content detection:
Experimental rat is not giving food in 12 hours after last 1 time administration, with 10% chloraldurate (0.2mL/kg),
Carry out anesthetized rat processing.Rat hindlimb is straightened patient outward, and exposure groin cuts off dorsal body setae, finds femoral pulse portion
Position, people's left hand tight rat neck of mouse clamp, the right hand further the tail of rat, tighten rat as far as possible, another people cuts off abdomen
Groin skin finds femoral artery, and heparin tube is close, takes blood about 5ml after cutting off femoral artery, is centrifuged 15min in 3500r/min,
Take supernatant therein spare, by its freezen protective, temperature (- 21 1 DEG C of scholar).It is required in strict accordance with kit, measures hs-CRP,
TNF-a, IL-6 content.
1.7 statistical method
All data carry out data processing and statistical analysis using SPSS17.0 statistics software, meet the meter of normal distribution
Amount data testing result indicates with mean ± standard deviation (± s), enters a SHR balance between the two groups variance analysis test before group, two groups
Data comparison among groups are examined with T, and more comparison among groups are with one-way analysis of variance (one-way ANOVA), if P < 0.05 is to have system
Meter learns difference, and P < 0.01 is to have significant statistical difference.
2 results
2.1 rat ordinary circumstances
After 6 weeks, there is the withered not gloss of hair color in SHR rat, and fluffy hair down, eyes are congested compared with other rats and color adds
Deep, the restless irritability of disposition, happiness is dynamic, good to fight with rat in cage, and disposition is classified many places, and (disposition changes point three-level: I grade refers in III grade
It catches to scream when holding neck and startle；II grade refers to catch and stings people when holding neck；III grade refers to scream when mentioning tail and startles or even sting people or big with cage
Mouse frequently fights).Gloss before each treatment group rat hair color is relatively administered, fluffy hair down is obviously improved, without obvious conjunctival congestion, property
The restless phenomenon of feelings is clearly better.
The situation of change of 2.2 each group rat medication anteroposterior contraction pressures
Before treatment, compared with blank control group, SHR each group systolic pressure rises obviously, be statistically significant (P <
0.01)；After treatment, each treatment group systolic pressure compared with model control group declines obvious (P < 0.O5)；Wherein, according to that
Puli organizes decline the most obviously (P < 0.01), is on close level with the decompression of kidney tonifying clearing liver high dose group, the two compares no significant difference
(P>0.O5)；Compared with low dose group, Amplitude of Hypotensive is bigger (P < 0.05) for kidney tonifying clearing liver height, middle dose group, in kidney tonifying clearing liver soup
For dosage group compared with low dose group, (P > 0.05) is on close level in decompression.
From the point of view of dose-effect relationship, in three dosage of drug groups, blood pressure is without obvious before low dose group is relatively administered for 1 week upon administration
Difference (P>0.05), after 2 weeks relatively be administered before be remarkably decreased (P<0.05), after 4 weeks, 6 weeks relatively be administered before it is extremely significant decline (P<
0.01)；Middle and high dosage group 1 week upon administration, 2 weeks, 4 weeks, 6 weeks before be administered compared with blood pressure, all extremely significant decline (P <
0.01).Experimental result is shown in Table 1.
1 each group rat medication anteroposterior contraction of table presses situation of change (± s, mmHg)
Note: comparison among groups: compared with blank control group before treatingaP < 0.01, after treatment compared with model control groupbP
< 0.05；Kidney tonifying clearing liver high dose group is in, compared with low dose groupcP<0.O5；In group relatively: compared with before administrationdP < 0.01
,eP<0.O5
2.3 couples of hs-CRP, the influence of TNF-a, IL-6
1. each group rat blood serum hs-CRP: compared with blank control group, model control group rises obviously, and difference has significant system
Meter learn meaning (P < 0.01), after treating, each treatment group blood pressure with model group compared with all decline significantly (P < 0.01), Chinese medicine group and
Blank control group compares no significant difference (P > 0.05), there were significant differences compared with blank control group for enalapril group (P <
0.01)；Wherein the decline of kidney tonifying clearing liver soup high dose group is the most obvious, and there were significant differences compared with enalapril group (P < 0.01),
Compared with the decline of kidney tonifying clearing liver middle dose group is horizontal not statistically significant (P > 0.05).
2. each group rat TNF-a: compared with blank control group, model control group blood pressure rises obvious (P < 0.01), passes through
After treatment, each treatment group blood pressure descending water head up display compared with model group writes (P < 0.01), but compared with blank control group, still gap
Significantly (P < 0.01)；Wherein the decline of kidney tonifying clearing liver soup high dose group is the most obvious, there were significant differences compared with enalapril group (P
< 0.01), compared with the decline of kidney tonifying clearing liver middle dose group is horizontal not statistically significant (P > 0.05).
3. each group rat IL-6: compared with blank control group, model group blood pressure increases significant (P < 0.01), after treating,
Each treatment group's blood pressure declines significant (P < 0.O1) compared with model control group, but compared with blank control group, still gap is significant
(P<0.01)；Wherein the decline of kidney tonifying clearing liver soup high dose group is horizontal maximum, there were significant differences compared with enalapril group (P <
0.01), compared with the decline of kidney tonifying clearing liver middle dose group is horizontal not statistically significant (P > 0.05).Experimental result is shown in Table 2.
2 each group rat blood serum inflammatory factor expression situation of table compare (N=4)
Note: compared with blank control groupaP < 0.01；Compared with model control groupbP < 0.01；With enalapril group ratio
Compared withcP < 0.01；
3 conclusions
The analysis of 3.1 animal models
It uses 12 week old SHR for model, is the animal model of genetic hypertension, the life with human essential hypertension disease
It is very much like to manage pathological development, is the current internationally recognized animal model closest to human essential hypertension.The disadvantage is that
SHR is mono- only costly, and SHR is selected to can yet be regarded as a kind of optimal model selection.
The influence of 3.2 pairs of blood pressures
Result of study shows: after Kidney-supplementing Qinggan decoction is treated 6 weeks, antihypertensive effect is obvious, but the WKY with Normal group
Group is compared, and systolic pressure still has certain gap, it may be said that the effect of decompression still has certain limitation；The decompression of Western medicine enalapril
Rapidly, amplitude is big, has been approached normal WKY control group blood pressure level by the 6th week.
Chinese medicine group reflects preferable antihypertensive effect, 6 Zhou Houming after giving kidney tonifying clearing liver soup therapeutic intervention 2 weeks
It is aobvious to reduce blood pressure；Wherein middle and high dosage group Amplitude of Hypotensive is larger, and stabilization is gradually depressured, and the two decompression is horizontal close, not shown bright
Aobvious dose-effect relationship, but the low dose group of ratio, antihypertensive effect is more significant, and apparent dose-effect relationship is presented；Low dosage is preceding two
All antihypertensive effects are not very obvious, and six Zhou Houcai gradually embody apparent antihypertensive effect.
There is diarrhea in Some Animals after observing high dose group administration in experiment, consider that this pharmaceutical composition is mostly the drop that clears liver
The product of fire, bitter cold injuring spleen-stomach, and traditional Chinese medicine liquid are concentrate, and concentration is bigger, matter is more sticky, then are not easy to absorb, influence digestion,
Diarrhea may be side effect caused by impairing the spleen and stomach.It is depressured, then drug should be adjusted to low dose of slowly steady to Long-term taking medicine
Decompression.
The influence of 3.3 pairs of inflammatory factors
Result of study shows: Chinese medicine group kidney tonifying clearing liver soup can be effectively reduced Serum hs-CRP, TNF-a, IL-6 inflammation because
It is sub horizontal；For Western medicine enalapril in terms of reducing inflammatory factor level, effect is clearly inferior to Chinese medicine group.The middle and high dosage group of Chinese medicine
Reducing inflammatory factor hs-CRP, it is curative for effect close in terms of TNF-a, IL-6, the two without apparent dose-effect relationship, ratio it is low
Then effect is more significant for dosage group, and there are apparent dose-effect relationships.
To sum up: on the one hand, the traditional chinese medicine composition of the invention (i.e. kidney tonifying clearing liver soup) can be effectively reduced blood while decompression
Clear inflammatory factor hs-CRP, TNF-a, IL-6 are horizontal, especially in use, high dose when, function and effect are more prominent, than
Western medicine go even farther；On the other hand, kidney tonifying clearing liver soup is initially fast fast not as good as Western medicine enalapril in terms of decompression
Speed, but can gradually slow steady blood pressure, it is the most prominent with middle and high dosage, can prevent from declining because of blood pressure it is too fast caused by it is dizzy,
The damage of the malaise symptoms and heart and brain organ of the postural hypotensions such as out of strength.
Obtained by results of animal: spontaneous hypertensive rat hs- can be effectively reduced in the traditional chinese medicine composition of the invention
The expression of CRP, TNF-a, IL-6 so that the inflammatory reaction access and EH that block it to participate in occur, the related mechanism of development, and are sent out
Waving it reduces the effect of SHR blood pressure.
From zoopery and typical case: the traditional chinese medicine composition of the invention can pass through in terms for the treatment of essential hypertension
Inhibit the expression of inflammatory factor to be gradually depressured, there is good effect, blood pressure not to rebound, have no toxic side effect, without pharmacological dependence after declining
The technical advantage of effect, while it can be obviously improved patient clinical symptom, such as headache, dizziness, insomnia, tinnitus, dysphoria, it mentions
The life quality of high patient provides a kind of new medicament selection for clinic.
Claims (10)
1. a kind of Chinese medicine composition for treating the reaction of essential hypertension complicated vessel wall inflammation, it is characterised in that: it is by following
The bulk pharmaceutical chemicals of weight proportion are prepared:
10-60 parts of Prunella vulgaris, 5-30 parts of radix scutellariae, 10-60 parts of wild chrysanthemum, 5-30 parts of Cortex Eucommiae, 10-60 parts of Eclipta prostrata, pheretima 5-30
Part, 10-60 parts of pueraria lobata, 5-30 parts of Radix Salviae Miltiorrhizae, 5-30 parts of radish seed, 5-30 parts of radix achyranthis bidentatae.
2. Chinese medicine composition according to claim 1, it is characterised in that: it is the bulk pharmaceutical chemicals preparation by following weight proportion
It forms:
30-40 parts of Prunella vulgaris, 10-20 parts of radix scutellariae, 15-40 parts of wild chrysanthemum, 15-20 parts of Cortex Eucommiae, 15-40 parts of Eclipta prostrata, pheretima 15-
20 parts, 30-40 parts of pueraria lobata, 15-30 parts of Radix Salviae Miltiorrhizae, 10-20 parts of radish seed, 10-20 parts of radix achyranthis bidentatae.
3. Chinese medicine composition according to claim 2, it is characterised in that: it is the bulk pharmaceutical chemicals preparation by following weight proportion
It forms:
30 parts of Prunella vulgaris, 10 parts of radix scutellariae, 15 parts of wild chrysanthemum, 15 parts of Cortex Eucommiae, 15 parts of Eclipta prostrata, 15 parts of pheretima, 30 parts of pueraria lobata, Radix Salviae Miltiorrhizae
15 parts, 12 parts of radish seed, 15 parts of radix achyranthis bidentatae.
4. Chinese medicine composition according to any one of claims 1 to 3, it is characterised in that: it be with Prunella vulgaris, radix scutellariae,
Wild chrysanthemum, Cortex Eucommiae, Eclipta prostrata, pheretima, pueraria lobata, Radix Salviae Miltiorrhizae, radish seed, the primary medicinal powder of radix achyranthis bidentatae or water extract be activity at
Point, the preparation that pharmaceutically acceptable auxiliary material or complementary ingredient are prepared is added.
5. Chinese medicine composition according to claim 4, it is characterised in that: the preparation is oral preparation.
6. Chinese medicine composition according to claim 5, it is characterised in that: the oral preparation is paste, pill, takes orally
Liquid, powder, tablet, granule or capsule.
7. the method for preparing Chinese medicine composition described in claim 1-6 any one, it is characterised in that: it includes the following steps:
A, the raw material of weight proportion described in claim 1 is weighed；
B, bulk pharmaceutical chemicals beat powder, or bulk pharmaceutical chemicals are added water to cook, and water cooking liquid concentration adds pharmaceutically acceptable auxiliary material or auxiliary
Property ingredient is prepared into pharmaceutically common preparation.
8. purposes of the Chinese medicine composition as claimed in any one of claims 1 to 6 in the drug of preparation treatment vascular wall inflammation.
9. use of the Chinese medicine composition as claimed in any one of claims 1 to 6 in the drug of preparation treatment essential hypertension
On the way.
10. purposes according to claim 8 or claim 9, it is characterised in that: the drug is treatment essential hypertension complicated blood
The drug of tube wall inflammatory reaction.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050026636A (en) * | 2003-09-09 | 2005-03-15 | 문조웅 | Mediicinal herbs composition for preventing and treating essential hypertension |
| CN105267612A (en) * | 2014-05-27 | 2016-01-27 | 李任 | Traditional Chinese medicinal decoction for treating hypertension |
| CN105833049A (en) * | 2016-04-05 | 2016-08-10 | 上海育生堂科技有限公司 | Medicinal composition for treating primary hypertension |
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2018
- 2018-12-21 CN CN201811572517.2A patent/CN109846927A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050026636A (en) * | 2003-09-09 | 2005-03-15 | 문조웅 | Mediicinal herbs composition for preventing and treating essential hypertension |
| CN105267612A (en) * | 2014-05-27 | 2016-01-27 | 李任 | Traditional Chinese medicinal decoction for treating hypertension |
| CN105833049A (en) * | 2016-04-05 | 2016-08-10 | 上海育生堂科技有限公司 | Medicinal composition for treating primary hypertension |
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| 章文春 等: "《中医学》", 30 June 2013, 人民军医出版社 * |
| 贾连群 等: "《常见心血管病的分子免疫基础与临床》", 31 July 2013, 中国医药科技出版社 * |
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