CN109845703A - A kind of separation method of artemia nauplii - Google Patents
A kind of separation method of artemia nauplii Download PDFInfo
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- CN109845703A CN109845703A CN201910029816.XA CN201910029816A CN109845703A CN 109845703 A CN109845703 A CN 109845703A CN 201910029816 A CN201910029816 A CN 201910029816A CN 109845703 A CN109845703 A CN 109845703A
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- artemia nauplii
- artemia
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Abstract
The invention belongs to biological products manufacture field, especially a kind of separation method of artemia nauplii, the separation method is the following steps are included: staticly settle 10 minutes；What collection artemia nauplii and part were mingled with does not hatch dead ovum and ghost；It is impregnated 30 seconds with liquor potassic permanganate；Fresh water dilution is added, hydrogen peroxide is added immediately after；It stirs evenly water body and adds hydrogen peroxide, upper layer ghost and dead ovum are removed after 3 minutes, according to separation degree repetitive operation one to twice；The artemia nauplii that centralized collection separation is completed；The present invention is on the basis of dissolved air flotation method and insufficient magnetic powder partition method, in conjunction with the advantage of two kinds of separation methods, provide it is a kind of it is economical and practical, be simple and efficient, applicability is wide, the fast separating process of lesser artemia nauplii is damaged to polypide.
Description
Technical field
The invention belongs to biological products manufacture field, especially a kind of separation method of artemia nauplii.
Background technique
Artemia nauplii is important biological feed during contemporary aquatic fry growing, because its nutritive value is higher, especially
It is that unsaturated fatty acid content is abundant, and palatability is good, easily absorbs, so becoming the excellent of the aquatic livestocks young such as shrimp, crab, fish
Bait is widely used in nursery production.Artemia eggs is under suitable temperature, salinity, illumination and dissolved oxygen conditions
Nauplius can be hatched, different egg hatch rates are different, and it is also different to correspond to unhatched dead ovum content.Artemia eggs and artemia
Nauplius separation is not thorough when just feeding aquatic seedling, and a large amount of artemia eggs are brought into nursery pond, can be pathogen and Zoothamnium
Class helminth provides the attachment base of Reproduction, also has part artemia eggs that can be taken in vivo by seedling, cannot digest and assimilate, even
Can be deposited in enteron aisle leads to death of seedling.
The separation method of artemia nauplii common at present has following four: (1) mechanical filter method: using specific mesh
Dredger be filtered；The shortcomings that being separated using mechanical filter is: artemia eggs ovum diameter is usually 220-300 microns, and artemia without
The length of the young is saved at 500-700 microns, width is at 180-250 microns, since the artemia eggs of different lines and nauplius are big
Small difference causes the strainer for being difficult to select suitable aperture；Aperture of filter screen is too big, and unhatched artemia eggs can also pass through, strainer
Aperture is too small, and part artemia nauplii can not pass through strainer；And artemia nauplii is not smooth circle, when filtering
It squeezes and shower can all damage nauplius, be broken a large amount of artemia nauplii appendages, nutriment is lost.(2) photo-induction method:
I.e. after the completion of artemia is hatched, the young is trapped with the method for photo-induction, then siphon obtains the young；This method is time-consuming and separation is imitated
Fruit is poor, cannot meet the needs of production, and the purity of artemia nauplii obtained generally can only achieve 75% left side after separation
The right side, it is this be mingled with the artemia larvae investment nursery ponds for not hatching artemia eggs and chorion largely after, do not hatch artemia eggs and chorion
It will largely be accumulated in nursery pond, very big harm is caused to the seedling of aquatic livestock, intestines stalk may occur after eating such as seedling
It blocks and dies or pathogenic organisms, such as trichobacteria and Zoothamnium are easily adhered in its surface, infect seedling, and then fry quality occur
Problem.(3) dissolved air flotation method: it is main by the addition chemical examples such as hydrogen peroxide or percarbonate, generation is decomposed in light using it
Bubble be attached to unhatched worm's ovum or ghost surface, draw high arrival liquid level by buoyancy, nauplius then passes through movement pendulum
De-bubbled sinks to bottom, thus achieve the purpose that nauplius separates, but hydrogen peroxide and carbon salt decomposition rate excessively are slower, separation
Required time is more long, and the movement of nauplius of the bubble that unhatched worm's ovum surface is formed vulnerable to surrounding influences, and causes a large amount of
Unhatched dead ovum continues to be mixed in cannot be completely separated among nauplius.(4) magnetic powder partition method: surface is wrapped when worm's ovum is processed
One layer of special magnetic powder is wrapped, the ghost that unhatched dead ovum and hatching are completed is concentrated using polarity after the completion of hatching and is received
Collection, to achieve the purpose that separate nauplius；It is using the shortcomings that Magnetic Isolation method: needs advanced technology by magnetic material
Matter is evenly coated in worm's ovum surface, and the enterprise that the current country possesses the technology is considerably less, and worm's ovum is expensive, the production circulated in the market
The product overwhelming majority does not use this technology.In addition, Chinese Patent Application No. 201610276290.1 discloses a kind of artemia without section children
The method of body hatching and quick separating, the separation method are more by being repeated in glass reinforced plastic sink with the bolting silk mesh bag of 120 mesh
Secondary whipping is thrown to the nauplius in mesh bag in glass reinforced plastic sink, and then achievees the effect that separate nauplius；The separation
Young separating rate can be improved in method, but this method is identical as mechanical filter method essence, in whipping and flushing process all
Nauplius can be damaged, a large amount of artemia nauplii appendages are broken, nutriment is lost.
Summary of the invention
In view of the deficiencies of the prior art, the present invention is on the basis of dissolved air flotation method and insufficient magnetic powder partition method, in conjunction with two
The advantage of kind of separation method, provide it is a kind of it is economical and practical, be simple and efficient, applicability is wide, lesser artemia is damaged to polypide without section
The fast separating process of the young.
The technical solution of the present invention is as follows:
A kind of separation method of artemia nauplii, the separation method the following steps are included:
Step A: after the completion of Artemia cysts hatching, stop air agitation, hatching barrel covers black lid, staticly settles 10 points
Clock；
Step B: opening the collection switch of hatching barrel lower end, collects artemia nauplii with dredger and what is be partially mingled with does not incubate
Change dead ovum and ghost；
Step C: by the artemia nauplii being collected into and be mingled with do not hatch dead ovum and ghost pours into separating barrel, use Gao Meng
Sour potassium solution impregnates 30 seconds, makes not hatch dead ovum and ghost surface attachment one layer of potassium permanganate of package；
Step D: fresh water dilution is added, hydrogen peroxide is added immediately after, reacts water body sufficiently, removes upper layer after 3 minutes
Ghost and dead ovum；
Step E: stirring evenly water body and add hydrogen peroxide, and upper layer ghost and dead ovum are removed after 3 minutes, repeats to grasp according to separation degree
Make one to twice；
Step F: the artemia nauplii that centralized collection separation is completed.
Further, the collection switch of hatching barrel lower end is opened to one third in step B.
Further, the mesh number of dredger is 200 mesh in step B.
Further, step B collects artemia nauplii and what is be partially mingled with does not hatch dead ovum and ghost until hatching barrel
Bottom cone is divided into only.
Further, the concentration of the potassium permanganate is 200ppm.
Further, after fresh water dilution is added in step D, the mass ratio of artemia nauplii and fresh water is 1:4.
Further, it is 0.3% that effective concentration after hydrogen peroxide mixes, which is added, in step D.
Further, it is 0.15% that step E, which adds effective concentration after hydrogen peroxide mixes,.
The principle of artemia nauplii separation method of the present invention is:
The present invention utilizes the advantage of magnetic powder partition method, in conjunction with the simple and fast of dissolved air flotation method, do not hatching dead ovum and is incubating
Change the ghost surface oxidation completed and superscribe one layer of potassium permanganate, recycles hydrogen peroxide and potassium permanganate generation redox anti-
It answers, generates bubble on the ghost surface for not hatching dead ovum and hatching completion, the water surface is drawn high under the action of buoyancy, artemia is without section
The young, which then passes through movement and gets rid of the bubble of surface generation, to continue to sink, to reach artemia without the separation for saving young nauplius.
The invention has the benefit that
(1) separation method separation degree of the present invention is high, and dead ovum ghost separation degree is up to 99%；
(2) quickly, separation process only needs 15 minutes for separation；
(3) easy to operate, at low cost, worm's ovum is without especially processing or manufacturing special separator；
(4) polypide damage is smaller, and individual is complete, and worm vigor is normal after separation；
(5) securely and reliably, separation process potassium permanganate and hydrogen peroxide can carry out disinfection to polypide surface sterilization, be allowed to conduct
Biological feed is more safe and reliable.
Specific embodiment
Specific embodiments of the present invention will be further explained below:
A kind of separation method of artemia nauplii, the separation method the following steps are included:
(1) after the completion of Artemia cysts hatching, stop air agitation, hatching barrel covers black lid, staticly settles 10 minutes；
(2) the collection switch for opening hatching barrel lower end, collects artemia nauplii with dredger and what is be partially mingled with does not hatch
Dead ovum and ghost；
(3) by the artemia nauplii being collected into and be mingled with do not hatch dead ovum and ghost pours into separating barrel, use permanganic acid
Potassium solution impregnates 30 seconds, makes not hatch dead ovum and ghost surface attachment one layer of potassium permanganate of package；
(4) fresh water dilution is added, hydrogen peroxide is added immediately after, reacts water body sufficiently, upper layer ghost is removed after 3 minutes
With dead ovum；
(5) it stirs evenly water body and adds hydrogen peroxide, upper layer ghost and dead ovum are removed after 3 minutes, according to separation degree repetitive operation
One to twice；
(6) artemia nauplii that centralized collection separation is completed.
Wherein, the collection switch of hatching barrel lower end is opened to one third in step B.
Wherein, the mesh number of dredger is 200 mesh in step B.
Wherein, step B collects artemia nauplii and what is be partially mingled with does not hatch dead ovum and ghost until hatching barrel bottom
Until conical section.
Wherein, the concentration of the potassium permanganate is 200ppm.
Wherein, after fresh water dilution is added in step D, the mass ratio of artemia nauplii and fresh water is 1:4.
Wherein, it is 0.3% that effective concentration after hydrogen peroxide mixes, which is added, in step D.
Wherein, it is 0.15% that step E, which adds effective concentration after hydrogen peroxide mixes,.
The above embodiments and description only illustrate the principle of the present invention and most preferred embodiment, is not departing from this
Under the premise of spirit and range, various changes and improvements may be made to the invention, these changes and improvements both fall within requirement and protect
In the scope of the invention of shield.
Claims (8)
1. a kind of separation method of artemia nauplii, it is characterised in that: the separation method the following steps are included:
Step A: after the completion of Artemia cysts hatching, stop air agitation, hatching barrel covers black lid, staticly settles 10 minutes；
Step B: opening the collection switch of hatching barrel lower end, collects artemia nauplii with dredger and what is be partially mingled with does not hatch extremely
Ovum and ghost；
Step C: by the artemia nauplii being collected into and be mingled with do not hatch dead ovum and ghost pours into separating barrel, use potassium permanganate
Solution impregnates 30 seconds, makes not hatch dead ovum and ghost surface attachment one layer of potassium permanganate of package；
Step D: fresh water dilution is added, hydrogen peroxide is added immediately after, reacts water body sufficiently, upper layer ghost is removed after 3 minutes
With dead ovum；
Step E: stirring evenly water body and add hydrogen peroxide, upper layer ghost and dead ovum is removed after 3 minutes, according to separation degree repetitive operation one
To twice；
Step F: the artemia nauplii that centralized collection separation is completed.
2. a kind of separation method of artemia nauplii according to claim 1, it is characterised in that: hatching barrel in step B
The collection switch of lower end is opened to one third.
3. a kind of separation method of artemia nauplii according to claim 1, it is characterised in that: dredger in step B
Mesh number is 200 mesh.
4. a kind of separation method of artemia nauplii according to claim 1, it is characterised in that: step B collects artemia
What nauplius and part were mingled with does not hatch dead ovum and ghost until hatching barrel bottom cone is divided into only.
5. a kind of separation method of artemia nauplii according to claim 1, it is characterised in that: the potassium permanganate
Concentration is 200ppm.
6. a kind of separation method of artemia nauplii according to claim 1, it is characterised in that: fresh water is added in step D
After dilution, the mass ratio of artemia nauplii and fresh water is 1:4.
7. a kind of separation method of artemia nauplii according to claim 1, it is characterised in that: dioxygen is added in step D
Effective concentration is 0.3% after water mixes.
8. a kind of separation method of artemia nauplii according to claim 1, it is characterised in that: step E adds dioxygen
Effective concentration is 0.15% after water mixes.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741990A (en) * | 2019-11-21 | 2020-02-04 | 中盐工程技术研究院有限公司 | Continuous separation device for artemia nauplii |
| CN113647347A (en) * | 2021-09-17 | 2021-11-16 | 广东海洋大学 | Early cultivation method of marine fish seedlings |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101786745A (en) * | 2010-03-05 | 2010-07-28 | 泉州师范学院 | Emergent large and medium-sized natural water body algae pollution remediation technique capable of resisting light stormy waves |
| CN103350026A (en) * | 2013-07-19 | 2013-10-16 | 中盐制盐工程技术研究院 | Method for separating artemia nauplii and unhatched artemia cysts |
| CN103651261A (en) * | 2012-09-21 | 2014-03-26 | 上海海洋大学 | Method for incubating brine shrimps through biological flocculating constituent suspension liquid |
-
2019
- 2019-01-14 CN CN201910029816.XA patent/CN109845703A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101786745A (en) * | 2010-03-05 | 2010-07-28 | 泉州师范学院 | Emergent large and medium-sized natural water body algae pollution remediation technique capable of resisting light stormy waves |
| CN103651261A (en) * | 2012-09-21 | 2014-03-26 | 上海海洋大学 | Method for incubating brine shrimps through biological flocculating constituent suspension liquid |
| CN103350026A (en) * | 2013-07-19 | 2013-10-16 | 中盐制盐工程技术研究院 | Method for separating artemia nauplii and unhatched artemia cysts |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741990A (en) * | 2019-11-21 | 2020-02-04 | 中盐工程技术研究院有限公司 | Continuous separation device for artemia nauplii |
| CN113647347A (en) * | 2021-09-17 | 2021-11-16 | 广东海洋大学 | Early cultivation method of marine fish seedlings |
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