CN109843435A - 用于细菌群落关系确定和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法 - Google Patents
用于细菌群落关系确定和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法 Download PDFInfo
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- CN109843435A CN109843435A CN201780059848.3A CN201780059848A CN109843435A CN 109843435 A CN109843435 A CN 109843435A CN 201780059848 A CN201780059848 A CN 201780059848A CN 109843435 A CN109843435 A CN 109843435A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0244—Drop counters; Drop formers using pins
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Rigid containers without fluid transport within
- B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/54—Labware with identification means
- B01L3/545—Labware with identification means for laboratory containers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
- B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
- B01J2219/00315—Microtiter plates
- B01J2219/00317—Microwell devices, i.e. having large numbers of wells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/0074—Biological products
- B01J2219/00743—Cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/021—Identification, e.g. bar codes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2300/0681—Filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/02—Identifying library members by their fixed physical location on a support or substrate
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662400841P | 2016-09-28 | 2016-09-28 | |
| US62/400,841 | 2016-09-28 | ||
| PCT/US2017/054108 WO2018064385A1 (en) | 2016-09-28 | 2017-09-28 | High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109843435A true CN109843435A (zh) | 2019-06-04 |
Family
ID=61760120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780059848.3A Pending CN109843435A (zh) | 2016-09-28 | 2017-09-28 | 用于细菌群落关系确定和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法 |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP3519096A4 (https=) |
| JP (1) | JP2019531730A (https=) |
| CN (1) | CN109843435A (https=) |
| AU (1) | AU2017335897A1 (https=) |
| CA (1) | CA3037631A1 (https=) |
| IL (1) | IL265540A (https=) |
| WO (1) | WO2018064385A1 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111483096A (zh) * | 2019-12-10 | 2020-08-04 | 中国科学院亚热带农业生态研究所 | 根际通道微流控芯片及其胶体块和一种制造胶体块的模具 |
| CN114207448A (zh) * | 2019-06-07 | 2022-03-18 | 蜂巢生物技术公司 | 用于收集、储存和分析生物样品的细胞盒 |
| CN115197840A (zh) * | 2022-08-17 | 2022-10-18 | 厦门大学 | 一种基于数字微流控技术的单细菌基因组测序方法 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3185619A1 (en) * | 2020-05-29 | 2021-12-02 | Riken | Method for treating cell population and method for analyzing genes included in cell population |
| EP3939699A1 (en) * | 2020-07-17 | 2022-01-19 | Biomillenia SAS | Growth modulation |
| WO2024135694A1 (ja) * | 2022-12-23 | 2024-06-27 | ペプチドリーム株式会社 | Pcrを用いたdnaの増幅方法 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1444043A (zh) * | 2002-12-25 | 2003-09-24 | 帕弗瑞生物技术(北京)有限公司 | 一种个体化特异性免疫细胞功能测定方法 |
| CN1472339A (zh) * | 2002-08-02 | 2004-02-04 | � 赵 | 高通量细胞生物芯片检测技术及试剂盒 |
| CN1920559A (zh) * | 2005-08-24 | 2007-02-28 | 赵翀 | 一种细胞生物技术、试剂盒及制备装置 |
| CN101421421A (zh) * | 2006-03-31 | 2009-04-29 | 利琴蒂亚有限公司 | 检测疱疹病毒的方法和微阵列 |
| CN105705659A (zh) * | 2013-08-28 | 2016-06-22 | 赛卢拉研究公司 | 大规模平行单细胞分析 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998045406A1 (en) * | 1997-04-09 | 1998-10-15 | Minnesota Mining And Manufacturing Company | Method and devices for partitioning biological sample liquids into microvolumes |
| US20050070005A1 (en) * | 1997-06-16 | 2005-03-31 | Martin Keller | High throughput or capillary-based screening for a bioactivity or biomolecule |
| US20050014155A1 (en) * | 2001-11-16 | 2005-01-20 | Xu Wilson C | High-density cell microarrays for parallel functional determinations |
| WO2005090549A1 (ja) * | 2004-03-19 | 2005-09-29 | Genomidea Inc. | 細胞培養用マイクロウェル |
| US9850482B2 (en) * | 2013-03-07 | 2017-12-26 | Seoul National University R&Db Foundation | Heterologous DNA barcoding method |
| EP2805769A1 (en) * | 2013-05-24 | 2014-11-26 | European Molecular Biology Laboratory | Methods for nano-scale single cell analysis |
| BR112015032512A8 (pt) * | 2013-06-27 | 2020-01-07 | 10X Genomics Inc | método de implementação de código de barras de materiais de amostra |
| JP6637885B2 (ja) * | 2013-07-21 | 2020-01-29 | ペンデュラム セラピューティクス, インコーポレイテッド | マイクロバイオームの特性解明、モニタリング、および処置のための方法およびシステム |
| AU2015209718B2 (en) * | 2014-01-25 | 2021-03-25 | Macrogen Inc. | Method and system for microbiome analysis |
| JP2017533723A (ja) * | 2014-11-17 | 2017-11-16 | イェダ リサーチ アンド ディベロップメント カンパニー リミテッドYeda Research And Development Co.Ltd. | マイクロバイオーム解析方法 |
| WO2016138496A1 (en) * | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| SG11201708579WA (en) * | 2015-04-21 | 2017-11-29 | Gen Automation Lab Tech Inc | High resolution systems, kits, apparatus, and methods for high throughput microbiology applications |
-
2017
- 2017-09-28 CA CA3037631A patent/CA3037631A1/en not_active Abandoned
- 2017-09-28 EP EP17857449.7A patent/EP3519096A4/en not_active Withdrawn
- 2017-09-28 AU AU2017335897A patent/AU2017335897A1/en not_active Abandoned
- 2017-09-28 CN CN201780059848.3A patent/CN109843435A/zh active Pending
- 2017-09-28 WO PCT/US2017/054108 patent/WO2018064385A1/en not_active Ceased
- 2017-09-28 JP JP2019516965A patent/JP2019531730A/ja active Pending
-
2019
- 2019-03-21 IL IL265540A patent/IL265540A/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1472339A (zh) * | 2002-08-02 | 2004-02-04 | � 赵 | 高通量细胞生物芯片检测技术及试剂盒 |
| CN1444043A (zh) * | 2002-12-25 | 2003-09-24 | 帕弗瑞生物技术(北京)有限公司 | 一种个体化特异性免疫细胞功能测定方法 |
| CN1920559A (zh) * | 2005-08-24 | 2007-02-28 | 赵翀 | 一种细胞生物技术、试剂盒及制备装置 |
| CN101421421A (zh) * | 2006-03-31 | 2009-04-29 | 利琴蒂亚有限公司 | 检测疱疹病毒的方法和微阵列 |
| CN105705659A (zh) * | 2013-08-28 | 2016-06-22 | 赛卢拉研究公司 | 大规模平行单细胞分析 |
Non-Patent Citations (2)
| Title |
|---|
| J DAISY VANITHA: ""Evaluation of microplate Alamar blue assay for drug susceptibility testing of Mycobacterium avium complex isolates"", 《DIAGN MICROBIOL INFECT DIS.》 * |
| 罗云波: "《食品生物技术导论》", 31 August 2016, 中国农业大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114207448A (zh) * | 2019-06-07 | 2022-03-18 | 蜂巢生物技术公司 | 用于收集、储存和分析生物样品的细胞盒 |
| CN111483096A (zh) * | 2019-12-10 | 2020-08-04 | 中国科学院亚热带农业生态研究所 | 根际通道微流控芯片及其胶体块和一种制造胶体块的模具 |
| CN111483096B (zh) * | 2019-12-10 | 2022-04-08 | 中国科学院亚热带农业生态研究所 | 根际通道微流控芯片及其胶体块和一种制造胶体块的模具 |
| CN115197840A (zh) * | 2022-08-17 | 2022-10-18 | 厦门大学 | 一种基于数字微流控技术的单细菌基因组测序方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2019531730A (ja) | 2019-11-07 |
| IL265540A (en) | 2019-05-30 |
| WO2018064385A1 (en) | 2018-04-05 |
| AU2017335897A1 (en) | 2019-04-04 |
| CA3037631A1 (en) | 2018-04-05 |
| EP3519096A1 (en) | 2019-08-07 |
| EP3519096A4 (en) | 2020-06-17 |
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Application publication date: 20190604 |