CN109827939A - A kind of optical fiber sensor device for microorganism detection - Google Patents
A kind of optical fiber sensor device for microorganism detection Download PDFInfo
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- CN109827939A CN109827939A CN201910234100.3A CN201910234100A CN109827939A CN 109827939 A CN109827939 A CN 109827939A CN 201910234100 A CN201910234100 A CN 201910234100A CN 109827939 A CN109827939 A CN 109827939A
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Abstract
The invention discloses a kind of optical fiber sensor devices for microorganism detection, including probe source, light detecting module, data processing module, database matching identification module and detection result display module, the probe source is connect with light detecting module, and the light detecting module is connect with data processing module.Structure of the invention is simple, it is easy to operate, testing result is accurate, detection accuracy is high, can the multiple-microorganism in sample be used for quickly detecting and be identified simultaneously, and it will test by display and come out as the result is shown, the detection of testing staff's real-time on-site and result is facilitated to read, without being limited by the field, and then improve the efficiency of microorganism detection work, in addition, the microorganism fluorescence signal of identification of failing is stored in unidentified database by the mode with digital signal, so that testing staff conducts further research analysis, help to find unknown new microbe.
Description
Technical field
The present invention relates to technical field of microbial detection more particularly to a kind of fibre optical sensor dresses for microorganism detection
It sets.
Background technique
Microorganism includes: one big including bacterium, virus, fungi and some small-sized protists, microalga etc.
Class biocenose, because body volume is very small, therefore, it is difficult to detect.But the detection of microorganism, facilitate in Epidemic outbreak of disease
When quickly determine pathogen, it is therefore significant to timely take counter-measure, control epidemic situation.
The existing detection for microorganism, mainly by micro- sem observation and both modes of spectrum analysis come into
Row, but micro- sem observation needs testing staff to have experience abundant, it is accurate to carry out to microorganism numerous in sample
Identification, and longer the time required to detecting, detection accuracy is low, also has a stringent limitation for detection place, and spectrum point
Analysis, although detection accuracy is relatively high, is also required to just can be carried out in specific space enrironment, cannot achieve real-time on-site inspection
It surveys, cannot obtain rapidly testing result at the first time, and it is slow to detect speed, cannot detect multiple-microorganism simultaneously.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, such as: the existing detection for microorganism,
It is mainly carried out by micro- sem observation and both modes of spectrum analysis, but micro- sem observation needs testing staff to have
Experience abundant could accurately identify microorganism numerous in sample, and longer the time required to detection, detection essence
Degree is low, also has stringent limitation for detection place, and spectrum analysis is also required to although detection accuracy is relatively high
It just can be carried out in specific space enrironment, cannot achieve real-time on-site detection, cannot obtain rapidly testing result at the first time, and
And detection speed is slow, cannot detect multiple-microorganism simultaneously.
To achieve the goals above, present invention employs following technical solutions:
A kind of optical fiber sensor device for microorganism detection, including probe source, light detecting module, data processing
Module, database matching identification module and detection result display module, the probe source is connect with light detecting module, described
Light detecting module is connect with data processing module, and the data processing module is connect with database matching identification module, described
Database matching identification module is connect with detection result display module.
Preferably, the probe source is for the irradiation sample to be detected that shines, the light source front installation of the probe source
There are colour filter and dichroic mirror.
Preferably, the light detecting module includes wavelength division multiplexer, fiber coupler and transmission fiber, and the wavelength-division is multiple
It is connected with the output end of device with the input terminal of fiber coupler, the output end of the fiber coupler and the input terminal of transmission fiber
Connection, the different fluorescence signals synthesis that the wavelength division multiplexer is used to reflect different microorganisms are a branch of along simple optical fiber
Transmission, the fiber coupler are used to that fluorescence signal to be coupled in optical fiber and be allocated or combine between different optical fiber,
The transmission fiber is used for transmission optical signal.
Preferably, the data processing module include demultiplexer, photoelectric detector, preamplifier, difference amplifier,
Filter and data acquisition module, the input terminal of the demultiplexer are connected with the output end of transmission fiber, the Photoelectric Detection
The input terminal of device is connected with the output end of demultiplexer, and the input terminal of the preamplifier and the output end of photoelectric detector connect
It connects, the input terminal of the difference amplifier is connected with the output end of preamplifier, and the input terminal and difference of the filter are put
The output end connection of big device, the input terminal of the data acquisition module are connected with the output end of filter, and the demultiplexer is used
It is separated in the optical signal for each different wave length for synthesizing wavelength division multiplexer, the photoelectric detector is for converting optical signal
At electric signal, the preamplifier is for amplifying ultra-weak electronic signal, reducing high-frequency noise and improving system signal noise ratio, the difference
Divide amplifier for amplifying to the difference between different frequency electric signal, the filter is used to carry out high frequency electrical signal
Filtering, the data acquisition module for acquire by demultiplexer, photoelectric detector, preamplifier, difference amplifier and
Electric signal after filter process.
Preferably, the database matching identification module includes data processor and data storage device, at the data
The input terminal of reason device is connected with the output end of data acquisition module, and the data processor is equipped with transmitted in both directions end, the number
It is connected according to processor with data storage device, the data processor is used for the collected electrical signal information of data acquisition module
It is converted to digital signal and is transmitted and handled.
Preferably, the data storage device includes microbiological data library and unidentified database, the data processor
Transmitted in both directions end connect with microbiological data library, the output end in the microbiological data library is connect with unidentified database, institute
Microbiological data library is stated for carrying out match cognization to digital signal and identifying that successful digital signal is transmitted back to data processing
Device, the unidentified database is for storing the digital signal identified not successfully.
Preferably, the detection result display module includes display, the input terminal and data processor of the display
Output end connection, the display is used to show successfully the microbiological data of match cognization.
The beneficial effects of the present invention are: structure of the invention is simple, easy to operate, testing result is accurate, and detection accuracy is high,
Can the multiple-microorganism in sample be used for quickly detecting and be identified simultaneously, and will test to go out as the result is shown by display
Come, facilitates the detection of testing staff's real-time on-site and result to read, without being limited by the field, and then improve microorganism detection work
The efficiency of work, in addition, the microorganism fluorescence signal for the identification that fails is stored in unidentified data by the mode with digital signal
In library, so that testing staff conducts further research analysis, help to find unknown new microbe.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the optical fiber sensor device for microorganism detection proposed by the present invention;
Fig. 2 is a kind of overall structure connection signal of the optical fiber sensor device for microorganism detection proposed by the present invention
Figure;
Fig. 3 is a kind of testing process schematic diagram of the optical fiber sensor device for microorganism detection proposed by the present invention.
In figure: 1 probe source, 2 optical fiber probe modules, 3 data processing modules, 4 database matching identification modules, 5 detections
It is result display module, 6 wavelength division multiplexers, 7 fiber couplers, 8 transmission fibers, 9 demultiplexers, 10 photoelectric detectors, 11 preposition
It is amplifier, 12 difference amplifiers, 13 filters, 14 data acquisition modules, 15 data processors, 16 data storage devices, 17 micro-
Biometric database, 18 unidentified databases, 19 displays.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
In the description of the present invention, it is to be understood that, term " on ", "lower", "front", "rear", "left", "right", "top",
The orientation or positional relationship of the instructions such as "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, merely to just
In description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with
Specific orientation construction and operation, therefore be not considered as limiting the invention.
Referring to Fig.1-3, a kind of optical fiber sensor device for microorganism detection, including probe source 1, light detect mould
Block 2, data processing module 3, database matching identification module 4 and detection result display module 5, which is characterized in that probe source 1
It is connect with light detecting module 2, light detecting module 2 is connect with data processing module 3, data processing module 3 and database
It is connected with identification module 4, database matching identification module 4 is connect with detection result display module 5, and probe source 1 is for shining
Sample to be detected is irradiated, provides light source illumination for the detection of microorganism in sample, semiconductor laser can be selected in probe source 1,
Under the irradiation of probe source 1, the different microorganisms in sample show different fluorescence.
Colour filter and dichroic mirror are installed in front of the light source of probe source 1, colour filter is set in the front of probe source 1,
To ensure that the light wave for providing correct wavelength filters out nonspecific wavelength using dichroic mirror, exciting light is only allowed to penetrate and obtain just
True detection, light detecting module 2 include wavelength division multiplexer 6, fiber coupler 7 and transmission fiber 8, wavelength division multiplexer 6 it is defeated
Outlet is connected with the input terminal of fiber coupler 7, and the output end of fiber coupler 7 is connected with the input terminal of transmission fiber 8, wavelength-division
The different fluorescence signals synthesis that multiplexer 6 is used to reflect different microorganisms is a branch of to transmit along simple optical fiber, sufficiently benefit
With the low-loss band of optical fiber, increase the transmission capacity of optical fiber, is doubled to the physical limits of an optical fiber transmission information
Several times.
Due to substantially reducing the usage amount of optical fiber, construction cost is greatly reduced, when a failure occurs, restores to get up
Convenient rapidly, fiber coupler 7 is used to that fluorescence signal to be coupled in optical fiber and be allocated or combine between different optical fiber,
Transmission fiber 8 is used for transmission optical signal, is coupled the fluorescence signal after the integration of wavelength division multiplexer 6 using fiber coupler 7
Into transmission fiber 8, to facilitate transmission fluorescence signal, data processing module 3 includes demultiplexer 9, photoelectric detector 10, preposition
Amplifier 11, difference amplifier 12, filter 13 and data acquisition module 14, the input terminal of demultiplexer 9 and transmission fiber 8
Output end connection, the input terminal of photoelectric detector 10 are connected with the output end of demultiplexer 9.
The input terminal of preamplifier 11 is connected with the output end of photoelectric detector 10, the input terminal of difference amplifier 12 and
The output end of preamplifier 11 connects, and the input terminal of filter 13 is connected with the output end of difference amplifier 12, data acquisition
The input terminal of module 14 is connected with the output end of filter 13, and demultiplexer 9 is used for each difference for synthesizing wavelength division multiplexer 6
The optical signal of wavelength separates, and the optical signal come to the transmission of transmission fiber 8 carries out parsing separation, is split into different wave length
Fluorescence signal makes it be restored to the state before wavelength division multiplexer 6 synthesizes, so as to subsequent detection.
Photoelectric detector 10 converts optical signals into electric signal, so that its is defeated for converting optical signals into electric signal
It is sent in circuit and carries out calculation process and analysis, to obtain the microbial information in sample, preamplifier 11 is for amplifying
Ultra-weak electronic signal reduces high-frequency noise and improves system signal noise ratio, and the electric signal that photoelectric detector 10 is converted out is amplified,
And eliminate the high-frequency noise generated in amplification process, influence of the HF noise signal to subsequent detection is dispelled, to improve inspection
The accuracy of result is surveyed, difference amplifier 12 makes it easy to point for amplifying to the difference between different frequency electric signal
Identification not, filter 13 for being filtered to high frequency electrical signal, data acquisition module 14 for acquire by demultiplexer 9,
Electric signal after photoelectric detector 10, preamplifier 11, difference amplifier 12 and the processing of filter 13.
Database matching identification module 4 include data processor 15 and data storage device 16, data processor 15 it is defeated
Enter end to connect with the output end of data acquisition module 14, data processor 15 is equipped with transmitted in both directions end, 15 He of data processor
Data storage device 16 connects, and A/D analog-digital converter is equipped in data processor 15, to convert the electrical signal to digital letter
Number, facilitate operation and processing, data processor 15 is for being converted to number to the collected electrical signal information of data acquisition module 14
Word signal is simultaneously transmitted and is handled, and data storage device 16 includes microbiological data library 17 and unidentified database 18, data
The transmitted in both directions end of processor 15 is connect with microbiological data library 17, the output end in microbiological data library 17 and unidentified database
18 connections.
Microbiological data library 17 is used to carry out match cognization to digital signal and will identify that successful digital signal is transmitted back to
Data processor 15, unidentified database 18 for storing to the digital signal identified not successfully, do not known successfully by collection
Other digital signal facilitates the new microorganism for finding unknown type so as to further progress research processing, and detection result is aobvious
Show that module 5 includes display 19, the digital signal for being transported to the successful match identification of data processor 15 is transferred to display
The input terminal of device 19, display 19 is connect with the output end of data processor 15, and display 19 is for showing successfully match cognization
Microbiological data.
In the present invention, when user uses the device, probe source 1, which shines, irradiates sample to be detected, by filtering accordingly
Color chips filters out the light wave of wrong wavelength, using dichroic mirror, filters out nonspecific wavelength, only exciting light is allowed to penetrate, and is detected
The microorganism that light source is irradiated to issues fluorescence, and different microorganisms issues different fluorescence signals, and fluorescence signal is through wavelength-division multiplex
Device 6 synthesizes the optical signal of certain wavelength, and is coupled in transmission fiber 8 through fiber coupler 7 to be transmitted, transmission fiber 8
The demultiplexer 9 of output connection carries out analysis dissociation to the optical signal received, is separated into the fluorescence of different wave length
Signal makes it be restored to the state between the synthesis of wavelength division multiplexer 6, is then communicated at photoelectric detector 10;
The fluorescence signal of different wave length is converted into the electric signal of different frequency by photoelectric detector 10, then by preceding
It sets amplifier 11 to amplify electric signal, and eliminates the high-frequency noise in power-off signal, then put by difference amplifier 12
The very much not difference between the electric signal of same frequency, becomes apparent it, facilitates subsequent detection, is filtered using filter 13
High frequency electrical signal is eliminated, the electric signal after 14 pairs of Usage data collection module filtering processings is acquired, is then transmitted
To data processor 15;
Data processor 15 converts the electrical signal to digital signal by A/D analog-digital converter, transmits in the form of data
In to microbiological data library 17 and existing known microorganisms information data carries out match cognization, the number of successful match identification
Signal is communicated back at data processor 15 together with recognition result, is then delivered at display 19 and is carried out testing result
It has been shown that, facilitates testing staff to read, the digital signal identified not successfully is stored in unidentified database 18, to detect people
Member conducts further research analysis, helps to find unknown new microbe, and structure of the invention is simple, easy to operate, inspection
It is accurate to survey result, detection accuracy is high, can the multiple-microorganism in sample is used for quickly detecting and be identified simultaneously, and by aobvious
Show that device 19 will test to come out as the result is shown, the detection of testing staff's real-time on-site and result is facilitated to read, without being limited by the field,
And then the efficiency of microorganism detection work is improved, in addition, the microorganism fluorescence signal for the identification that fails is by with digital signal
Mode be stored in unidentified database 18.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of optical fiber sensor device for microorganism detection, including probe source (1), light detecting module (2), data
Processing module (3), database matching identification module (4) and detection result display module (5), which is characterized in that the detection light
Source (1) is connect with light detecting module (2), and the light detecting module (2) connect with data processing module (3), the data
Processing module (3) is connect with database matching identification module (4), and the database matching identification module (4) and detection result are aobvious
Show that module (5) connect.
2. a kind of optical fiber sensor device for microorganism detection according to claim 1, which is characterized in that the spy
Light source (1) is surveyed for the irradiation sample to be detected that shines, the light source front of the probe source (1) is equipped with colour filter and two colors
Mirror.
3. a kind of optical fiber sensor device for microorganism detection according to claim 1, which is characterized in that the light
Line detecting module (2) includes wavelength division multiplexer (6), fiber coupler (7) and transmission fiber (8), the wavelength division multiplexer (6)
Output end is connected with the input terminal of fiber coupler (7), the output end of the fiber coupler (7) and transmission fiber (8) it is defeated
Enter end connection, the different fluorescence signals synthesis that the wavelength division multiplexer (6) is used to reflect different microorganism it is a branch of with along
Simple optical fiber transmission, fluorescence signal for being coupled in optical fiber and carrying out between different optical fiber by the fiber coupler (7)
Distribution or combination, the transmission fiber (8) are used for transmission optical signal.
4. a kind of optical fiber sensor device for microorganism detection according to claim 3, which is characterized in that the number
It include demultiplexer (9), photoelectric detector (10), preamplifier (11), difference amplifier (12), filter according to processing module (3)
The input terminal of wave device (13) and data acquisition module (14), the demultiplexer (9) is connected with the output end of transmission fiber (8),
The input terminal of the photoelectric detector (10) is connected with the output end of demultiplexer (9), the input of the preamplifier (11)
End is connected with the output end of photoelectric detector (10), the input terminal of the difference amplifier (12) and preamplifier (11) it is defeated
Outlet connection, the input terminal of the filter (13) are connected with the output end of difference amplifier (12), the data acquisition module
(14) input terminal is connected with the output end of filter (13), what the demultiplexer (9) was used to synthesize wavelength division multiplexer (6)
The optical signal of each different wave length separates, and the photoelectric detector (10) is for converting optical signals into electric signal, before described
It sets amplifier (11) to be used to amplify ultra-weak electronic signal, reduce high-frequency noise and improve system signal noise ratio, the difference amplifier
(12) for amplifying to the difference between different frequency electric signal, the filter (13) is used to carry out high frequency electrical signal
Filtering, the data acquisition module (14) pass through demultiplexer (9), photoelectric detector (10), preamplifier for acquiring
(11), the electric signal after difference amplifier (12) and filter (13) are handled.
5. a kind of optical fiber sensor device for microorganism detection according to claim 4, which is characterized in that the number
It include data processor (15) and data storage device (16) according to storehouse matching identification module (4), the data processor (15)
Input terminal is connected with the output end of data acquisition module (14), and the data processor (15) is equipped with transmitted in both directions end, described
Data processor (15) and data storage device (16) connection, the data processor (15) are used for data acquisition module (14)
Collected electrical signal information is converted to digital signal and is transmitted and handled.
6. a kind of optical fiber sensor device for microorganism detection according to claim 5, which is characterized in that the number
Include microbiological data library (17) and unidentified database (18) according to storage device (16), the data processor (15) it is two-way
Transmission end is connect with microbiological data library (17), and the output end of the microbiological data library (17) and unidentified database (18) are even
It connects, the microbiological data library (17) is used to carry out match cognization to digital signal and will identify that successful digital signal is transmitted back to
Data processor (15), the unidentified database (18) is for storing the digital signal identified not successfully.
7. a kind of optical fiber sensor device for microorganism detection according to claim 5, which is characterized in that the spy
Surveying result display module (5) includes display (19), the input terminal of the display (19) and the output of data processor (15)
End connection, the display (19) are used to show successfully the microbiological data of match cognization.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111548902A (en) * | 2020-04-07 | 2020-08-18 | 广东环凯生物科技有限公司 | Microorganism detection system and method |
CN111896230A (en) * | 2020-08-10 | 2020-11-06 | 西安工业大学 | Dynamic MTF transfer function detection system of photoelectric imaging system |
CN112268886A (en) * | 2020-09-04 | 2021-01-26 | 武汉光谷航天三江激光产业技术研究院有限公司 | Laser rapid detection and disinfection integrated device and method for virus and bacteria |
CN114724142A (en) * | 2022-04-02 | 2022-07-08 | 四川大学 | Sewage treatment indicative microorganism image identification method based on convolutional neural network |
-
2019
- 2019-03-26 CN CN201910234100.3A patent/CN109827939A/en not_active Withdrawn
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111548902A (en) * | 2020-04-07 | 2020-08-18 | 广东环凯生物科技有限公司 | Microorganism detection system and method |
CN111896230A (en) * | 2020-08-10 | 2020-11-06 | 西安工业大学 | Dynamic MTF transfer function detection system of photoelectric imaging system |
CN112268886A (en) * | 2020-09-04 | 2021-01-26 | 武汉光谷航天三江激光产业技术研究院有限公司 | Laser rapid detection and disinfection integrated device and method for virus and bacteria |
CN114724142A (en) * | 2022-04-02 | 2022-07-08 | 四川大学 | Sewage treatment indicative microorganism image identification method based on convolutional neural network |
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Application publication date: 20190531 |