CN1098139A - Utilize and extract Potenlini waste liquid breed chlorella technology and device - Google Patents
Utilize and extract Potenlini waste liquid breed chlorella technology and device Download PDFInfo
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- CN1098139A CN1098139A CN93108855A CN93108855A CN1098139A CN 1098139 A CN1098139 A CN 1098139A CN 93108855 A CN93108855 A CN 93108855A CN 93108855 A CN93108855 A CN 93108855A CN 1098139 A CN1098139 A CN 1098139A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
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- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
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Abstract
The present invention relates to a kind of utilization and extract Potenlini waste liquid breed chlorella technology and device, acid waste water pH2.5～3 of Potenlini will be extracted, carry out spontaneous fermentation, Radix Glycyrrhizae sour water after the fermentation transfers to pH5～7 for 10 times with clean clear water dilution, add 5 ‰ bone meal water mixings then after heat up naturally when making culture-liquid temp reach 20～40 ℃, add that the kind algae of cultivating through secondary inoculates, breeding process temperature controlling, the correction of pH value, CO
2Replenish and get rid of excess oxygen, inoculation is to carry out in gas exchanger, and this interchanger links to each other with pipeline reactor in being installed in the greenhouse, but whole process is the breed chlorella technology in a best habitat of totally-enclosed, round-the-clock simulating nature, the product grade height, with low cost.
Description
The present invention relates to a kind of utilization and extract Potenlini waste liquid breed chlorella technology and device.
Chlorella is a kind of aquatic algae that contains high protein, contain 50% crude protein and about 10% fat in the dried according to surveying and determination frond, will exceed 5～6 times than food crop, the protein effectiveness coefficient of chlorella is bordering on animal protein, can absorb for animal well.
Propagate artificially chlorella from 1940's at first by the European set about research, technology and technology are being updated in continuous exploratory experiment between decades, EP0239272 is about improvement one literary composition of biomass production, the production that discloses marine alga is to carry out in a solid translucent or transparent straight tube, the outside surface of pipe exposes in the sun, and has equipped the device that can make the light transmission tube core.Cultivation one literary composition of SU1565-884 chlorella, disclose wheat tooth extract solution, or 1: 1 mixture of 2% extract, with 0.5% cottonseed oil cake solution of extract nutritive medium as the cultivation chlorella with 2～4%, cultivation is to carry out in the sun, constantly stirs to raise the efficiency simultaneously.EP0442522 cultivates the method for chlorella, has disclosed with the whey waste liquid that abandons, and turns out the various chlorellas of high yield, high reproductive rate.JP60180578 chlorella nutrient solution and cultural method, disclosed germanic solution has been added to unpurified skimmed soy beans goods, or with the mixture of soybean of brewageing or vinegar in the germanic nutrient solution of preparation, the existence of germanium has suppressed the breeding of various bacteriums in the nutrient solution, and can only make the chlorella breeding.In sum, improve and development continuous the cultural technique of chlorella many countries and regions, has built up various types of cultivation apparatus, and formed little algae industry.
At brulee, on the new rock surface and the earth in some extremely harsh habitats, what intrusion was settled down the earliest is exactly algae, big and small lake in the desert, seasonal pool, the procreation of living year after year of the very indomitable biology of a large amount of vitality is all arranged on the soak, wherein serving as dominant role finishes the primary and produces that to make proteinic for other is biological be exactly various algae, since ancient times, the locals allays one's hunger with it and is referred to as whole food, China has the big area desert region, the fertility that arid and Desertification cause descends, troubling wide geographic area, develop Desertification area nature and ecological resources, become numerous scientific workers' research topic.
Endemic plant Radix Glycyrrhizae in the desert, it is a kind of wild plant resource of preciousness, effective constituent of many uses is worth very high, annual Potenlini outlet of being extracted by Radix Glycyrrhizae processing industry exchanges a large amount of foreign exchanges for, contain many beneficiating ingredients at the waste liquid that extracts the discharging of Potenlini process, xitix 11.0～31.2mg/l is wherein arranged, also have protein 6.2～10.2%, class fat 0.2～4.7%, asparagine 1.0～4.0%, resin 1.5～6.5%, resinoid 1.75～4.2%, bitter principle 1.8～4.0%, chromocor compound 3～4%, sterone compound 1.5～2.0%, glucose 0.6～15.2%, sucrose 0.3～11%, starch 34%, Mierocrystalline cellulose 9.7～23.2%, pH2.5～3, the discharging of this acid waste water is land occupation not only, and causes pollution in various degree.
The objective of the invention is to overcome the deficiency of prior art, make the no waste production of Radix Glycyrrhizae become possibility, and in conjunction with peculiar nature of desert area and Biological resources, provide a kind of the utilization to extract waste liquid breed chlorella technology and the device that Potenlini is discharged, make uncultivated sunlight desert barren land become fertile soil, change existing ecotope.Another object of the present invention is to utilize the reaction of modern technologies biological duct to create the chamber operation of artificial environment, provide a kind of totally-enclosed, round-the-clock cultural technique of imitating the best habitat of nature, the chlorella that production can be edible.
Implementing technical scheme of the present invention is:
1, breeding technique and device
The acid waste water that extracts the Potenlini discharging enters receiving tank (11) and puts into natural fermented pond (12) then, acid waste water after fermentation enters Preparation tank (13), be blended into clean clear water and become basic culture solution, put into bin stock narrow-necked earthen jar (3) then, add at this and to send into gas exchanger (4) after 5 ‰ bone meal water (10) mix, this interchanger (4) and pipeline reactor (2) and CO
2Producer (5) exit end links to each other, temperature controlling, the correction of pH value, the inoculation of kind algae, CO
2Gas make-up and eliminating excess oxygen are all carried out in gas exchanger (4), its bottom taperedly links to each other with water pump, send in the pipeline reactor (2) with the nutrient solution in the gas exchanger (4) or through postvaccinal nutrient solution, flow velocity is 1 meter per second, form the system flow circulation to finish photoresponse, dark reaction, promote that chlorella grows in moderate habitat.Biological duct reactor (2) is installed in the greenhouse (1), it is the two-layer U-shaped coiled pipe composition that is arranged in parallel up and down, every unit is by some long 2～3m, standard transparent glass pipe and the elbow connection of diameter 50～120mm form, pipeline connection is to be assembled into one with supporting packing ring and flange, and be fixed on the steelframe the slightly gradient, collector (7) is equipped with in pipeline reactor (2) outlet, make chlorella totally-enclosed, grow under all weather conditions, can avoid dust, assorted bacterium, insect and other pollutent are to the pollution of suspension, the product grade height can make CO in addition
2Be utilized effectively in order to avoid scatter and disappear, be convenient to clean the inwall of bio-reactor simultaneously, the sponge choker block of regularly packing in tubing system will clean up attached to the suspension liquid on the inwall to guarantee to be subjected to luminosity.
2, nutrient solution preparation
The acid waste water pH that extracts the Potenlini discharging is 2.5～3, put into fermentation vat (12) from receiving tank (11) and carry out spontaneous fermentation, acid waste water after fermentation enters nutrient solution Preparation tank (13), in waste water and the dilution in 1: 10 of clean clear water ratio, the pH value is transferred to 5～7 as basic culture solution, put into the bin stock narrow-necked earthen jar then and add 5 ‰ bone meal water, the slurry that this pipe powder water system is mixed with in the solid-liquid ratio by bone meal and water purification at 1: 20 is after boiling and get.
3, CO
2Producer
Algal culture process mass consumption CO
2, quite general green plants consumption can be how 20 times, needs constantly to replenish CO
2Gas, this CO
2Producer (5) is to utilize cheap bicarbonate of ammonia and strong sulfuric acid response, the CO of generation
2It is interior as the gas make-up source that pneumatic transmission is gone into gas exchanger (4), and the ammonium sulfate of generation can be used as fertilizer and uses.
4, algal species cultivation
Planting algae (cholorelia) is that one-level is cultivated in indoor elder generation with the agar-agar slant culture, enter after 30～40 days and carry out the secondary cultivation in the carboy, reached finite concentration 500～1,000 ten thousand cells/cc after 30～40 days, inoculation in gas exchanger (4) again is by being pumped in the system.Inoculation should be carried out to keep out of the direct sun the frustule decolouring taking place at night, inoculates in back 72 hours and will take the sunshade measure, prevents photoshock, and temperature is 20～40 ℃.
The present invention compared with prior art has following characteristics:
1, the present invention utilizes and extracts Potenlini waste water breed chlorella, be the more efficiently fixing more luminous energy of a kind of more high green plants of Desertification area developing, make human those uncultivated sunbelt areas that seldom utilize up to now carry out the suitability for industrialized production algae, the change barren land is fertile soil, for the mankind provide more food.
2, the present invention adopts the reaction of modern technologies biological duct to create the chamber operation of artificial environment, can imitate the best habitat of nature, finishes totally-enclosed, the round-the-clock continuous automatic production process of chlorella, has improved productivity, and the product grade height and the cost of production are low.
3, production technique of the present invention, make the concentration processing of Radix Glycyrrhizae and do not have the waste production technique become a kind of may, technology of the present invention and device equally also be applicable to spirulina,
The breed of microalgaes such as row algae and salt algae is for the development and utilization of saline and alkaline region resource provides foundation.
Description of drawings:
Fig. 1 utilizes Potenlini waste water to culture chlorella technology and device schema
Diagram: 1-greenhouse 2-pipeline reactor 3-bin stock narrow-necked earthen jar 4-gas exchanger 5-CO
2The natural fermented pond of producer 6-pump 7-collector 8-source of artificial light 9-bone meal 10-bone meal water pulp digester 11-receiving tank 12-13-Preparation tank 14-Potenlini extracts the workshop
Embodiment:
Pilot plant is the glasshouse of long 25m, a wide 4m, and bio-reactor is installed in indoor, and bio-reactor is by normal glass pipeline and elbow and ring flange gang, internal diameter of the pipeline 51mm, and external diameter 61mm, length overall is 380mm.
The nutrient solution of 350 liters of capacity preparing is injected in the reactor that passes through cleaning and sterilizing, heats up through nature that to make culture-liquid temp be about 25 ℃, 100 liters of inoculations of the kind algae that will cultivate then.The beginning vacuum primingpump stirs propelling with nutrient solution, forms turbulent flow in reactor, and flow velocity is 1 meter per second.Between reactor and gas exchanger, CO is installed
2Producer is used for CO
2Fertilising.In the reactor terminal, one of stainless steel collector is installed, at the filter cloth in the funnel support shop of collector, the filter cloth hole is about 60 orders,, with after the drip washing of gravity filtration gained algae mud process, concentrate with gravity filtration process more again through after the drip washing through gravity filtration gained algae mud, through dehydrate dry algae powder, output is 20g/1000cc.
Claims (8)
1, a kind of utilization is extracted the Potenlini waste liquid and is cultured chlorella technology and device, it is characterized in that and to extract acid waste water PH2.5～3 of Potenlini discharging, put into fermentation vat (12) from collector (11) and carry out spontaneous fermentation, Radix Glycyrrhizae sour water after the fermentation adds clean clear water dilution with 1: 10 ratio, transferring to PH5～7 sends in the bin stock narrow-necked earthen jar (3) as basic culture solution (13), the bone meal water (10) that adds 5 ‰ (V) then, send into gas exchanger (4) behind the mixing, send in the pipeline reactor (2) by the pump (6) that the bottom connects, when making culture-liquid temp reach 20～40 ℃ through heating up naturally, in gas exchanger (4), add the kind algae of cultivating through secondary and inoculate this interchanger (4) and pipeline reactor (2) CO
2The outlet of producer (5) links to each other, the correction of temperature controlling, pH value, CO
2Replenish and get rid of excess oxygen and all in gas exchanger (4), carry out, nutrient solution or through the nutrient solution of inoculation, the pump (6) by conical lower portion pumps in the pipeline reactor, forms turbulent dynamic circulation process.
2, technology as claimed in claim 1 and device is characterized in that described bone meal water system adds water with bone meal by solid-to-liquid ratio and is made into slurry at 1: 20, and then carry out boiling.
3, technology as claimed in claim 1 and device is characterized in that described carbonic acid gas ties up to CO
2In the producer (5), be raw material, through the CO of chemical reaction generation with the bicarbonate of ammonia and the vitriol oil
2, the ammonium sulfate of generation is made fertilizer.
4, technology as claimed in claim 1 and device, it is characterized in that described inoculation is through the agar-agar slant culture after 30～40 days with kind of algae, putting into the carboy secondary cultivates when reaching 500～1,000 ten thousand cells/cc after 30～40 days, in gas exchanger, inoculate, inoculation should be carried out at night, inoculated in back 72 hours and will take the sunshade measure.
5, a kind of utilization is extracted the Potenlini waste liquid and is cultured chlorella technology and device, it is characterized in that in the greenhouse that simple glass and steelframe build up, the upper and lower two-layer glass pipe bio-reactor that is arranged in parallel by the U-shaped coiled pipe is installed, every unit is formed by the normal glass pipeline and the elbow connection of 50～120 millimeters of some long 2～3 meters, diameters, pipe joint is that ring flange connects, reactor is fixed on the steelframe the slightly gradient, pipe outlet connects gas exchanger and collector, this interchanger bottom is tapered to link to each other CO with water pump
2Producer also links to each other with interchanger with nutrient solution bin stock narrow-necked earthen jar, and nutrient solution constantly pumps into reactor since then and forms the liquid-flow circulation.
6, technology as claimed in claim 5 and device is characterized in that described pump, bin stock narrow-necked earthen jar, gas exchanger, collector are Stainless Steel Products, and the stainless steel vacuum primingpump pumps into pipeline reactor with the nutrient solution in the gas exchanger, and linear velocity is 1 meter per second.
7, technology as claimed in claim 5 and device is characterized in that middle part, greenhouse and left and right sides wing-rooms on either side of a one-story house are equipped with 1000 watts of tungsten-iodine lamps, and the illuminance scope is 700～2000 luxs.
8, technology as claimed in claim 5 and device is characterized in that pipeline reactor cleans, and are the sponge choker blocks of regularly packing in tubing system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93108855A CN1098139A (en) | 1993-07-26 | 1993-07-26 | Utilize and extract Potenlini waste liquid breed chlorella technology and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93108855A CN1098139A (en) | 1993-07-26 | 1993-07-26 | Utilize and extract Potenlini waste liquid breed chlorella technology and device |
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| Publication Number | Publication Date |
|---|---|
| CN1098139A true CN1098139A (en) | 1995-02-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93108855A Pending CN1098139A (en) | 1993-07-26 | 1993-07-26 | Utilize and extract Potenlini waste liquid breed chlorella technology and device |
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| CN (1) | CN1098139A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559478A (en) * | 2012-01-18 | 2012-07-11 | 杭州鑫伟低碳技术研发有限公司 | Controllable slope type microalgae cultivation system and microalgae cultivation method thereof |
| CN103038332A (en) * | 2010-05-12 | 2013-04-10 | 美国科乐生物能源有限公司 | Cultivation of photosynthetic organisms |
| CN106922153A (en) * | 2014-08-25 | 2017-07-04 | 全谱实验室有限公司 | The apparatus and method produced while for cannabinoid compounds |
| CN108249574A (en) * | 2017-12-13 | 2018-07-06 | 华南理工大学 | It is a kind of to handle pig farm breeding wastewater using microalgae and produce the device and its technique of algae powder |
| CN111164197A (en) * | 2017-10-10 | 2020-05-15 | 智康工程顾问有限公司 | Methods and systems for heterotrophic and mixotrophic culture of microalgae |
-
1993
- 1993-07-26 CN CN93108855A patent/CN1098139A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103038332A (en) * | 2010-05-12 | 2013-04-10 | 美国科乐生物能源有限公司 | Cultivation of photosynthetic organisms |
| CN102559478A (en) * | 2012-01-18 | 2012-07-11 | 杭州鑫伟低碳技术研发有限公司 | Controllable slope type microalgae cultivation system and microalgae cultivation method thereof |
| CN102559478B (en) * | 2012-01-18 | 2013-08-28 | 杭州鑫伟低碳技术研发有限公司 | Controllable slope type microalgae cultivation system and microalgae cultivation method thereof |
| CN106922153A (en) * | 2014-08-25 | 2017-07-04 | 全谱实验室有限公司 | The apparatus and method produced while for cannabinoid compounds |
| CN111164197A (en) * | 2017-10-10 | 2020-05-15 | 智康工程顾问有限公司 | Methods and systems for heterotrophic and mixotrophic culture of microalgae |
| CN111164197B (en) * | 2017-10-10 | 2024-03-22 | 智康工程顾问有限公司 | Methods and systems for heterotrophic and mixotrophic culture of microalgae |
| CN108249574A (en) * | 2017-12-13 | 2018-07-06 | 华南理工大学 | It is a kind of to handle pig farm breeding wastewater using microalgae and produce the device and its technique of algae powder |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |